##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211209_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11251321 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52675396960055 31.0 31.0 34.0 30.0 34.0 2 31.547550372085198 31.0 31.0 34.0 29.0 34.0 3 31.60891134472121 33.0 31.0 34.0 28.0 34.0 4 35.21499128857847 37.0 35.0 37.0 32.0 37.0 5 35.28056847724814 37.0 35.0 37.0 33.0 37.0 6 35.34120722357846 37.0 35.0 37.0 32.0 37.0 7 35.25818070606998 37.0 35.0 37.0 32.0 37.0 8 35.27418460463443 37.0 35.0 37.0 32.0 37.0 9 36.83599188042009 39.0 37.0 39.0 32.0 39.0 10-11 36.79813099279632 39.0 37.0 39.0 32.0 39.0 12-13 36.6686604621804 39.0 37.0 39.0 32.0 39.0 14-15 37.843558725237685 40.0 37.0 41.0 32.0 41.0 16-17 37.83078515847161 40.0 37.0 41.0 32.0 41.0 18-19 37.85560108897435 40.0 37.0 41.0 32.0 41.0 20-21 37.8281203158278 40.0 37.0 41.0 32.0 41.0 22-23 37.70507147560717 40.0 37.0 41.0 32.0 41.0 24-25 37.59580679459772 40.0 37.0 41.0 32.0 41.0 26-27 37.430858163232564 40.0 36.5 41.0 31.5 41.0 28-29 37.329139085090546 40.0 36.0 41.0 31.0 41.0 30-31 37.18074393220138 39.5 36.0 41.0 31.0 41.0 32-33 37.00238443112591 39.0 36.0 41.0 30.5 41.0 34-35 36.857086914505416 39.0 35.0 41.0 30.0 41.0 36-37 36.75122454510009 39.0 35.0 41.0 30.0 41.0 38-39 36.593055339901866 39.0 35.0 41.0 30.0 41.0 40-41 36.4116661056955 39.0 35.0 40.0 29.5 41.0 42-43 36.187493361890574 39.0 35.0 40.0 29.0 41.0 44-45 35.90962536754573 38.0 35.0 40.0 28.0 41.0 46-47 35.622650175921564 38.0 34.0 40.0 27.0 41.0 48-49 35.362361184077855 38.0 34.0 40.0 26.0 41.0 50-51 34.62477632626427 37.5 33.5 39.5 25.5 40.5 52-53 34.689144279147314 37.5 33.5 39.5 26.0 40.5 54-55 35.127399707109944 38.0 34.0 40.0 26.0 41.0 56-57 35.02070165805419 37.0 34.0 40.0 26.0 41.0 58-59 34.75056208955375 37.0 34.0 40.0 26.0 41.0 60-61 34.51416571440811 36.0 33.5 40.0 26.0 41.0 62-63 34.19601756096017 36.0 33.0 39.0 26.0 41.0 64-65 33.93494283915639 35.0 33.0 39.0 25.5 41.0 66-67 33.770437977905 35.0 33.0 39.0 25.0 41.0 68-69 33.49851168587226 35.0 33.0 38.0 25.0 41.0 70-71 33.26983582638874 35.0 33.0 37.5 25.0 40.0 72-73 33.002589740351375 35.0 32.5 37.0 24.0 40.0 74-75 32.7113242080641 35.0 32.0 36.5 24.0 39.5 76-77 32.44325772947017 35.0 32.0 36.0 23.5 39.0 78-79 32.149540129554566 35.0 31.5 36.0 23.0 39.0 80-81 31.905729513894414 35.0 31.0 35.0 22.5 37.5 82-83 31.64507460946141 35.0 31.0 35.0 21.5 37.0 84-85 31.43450720142106 35.0 31.0 35.0 20.0 37.0 86-87 31.23812301684398 34.0 31.0 35.0 20.0 36.0 88-89 31.065644469658274 34.0 31.0 35.0 20.0 36.0 90-91 30.844676816171187 34.0 30.5 35.0 19.5 36.0 92-93 30.64467341212645 34.0 30.0 35.0 19.0 35.5 94-95 30.503510210045558 34.0 30.0 35.0 18.5 35.0 96-97 30.322874220724838 34.0 29.5 35.0 18.0 35.0 98-99 30.100825449740523 34.0 29.0 35.0 18.0 35.0 100 28.90987031656105 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4418745509310824 1101 2 -0.41520655717925337 1101 3 -0.4203008213319812 1101 4 -0.33515524453813583 1101 5 -0.3019371062135363 1101 6 -0.32037116641844676 1101 7 -0.3392575232240418 1101 8 -0.32392320680503417 1101 9 -0.551443570375092 1101 10-11 -0.5004323509299269 1101 12-13 -0.5509261154779992 1101 14-15 -0.7491885351031158 1101 16-17 -0.7758698346846984 1101 18-19 -0.6967823667945723 1101 20-21 -0.7468788984395545 1101 22-23 -0.8013636529934303 1101 24-25 -0.8272736140199797 1101 26-27 -0.8313801155439577 1101 28-29 -0.7769305450405781 1101 30-31 -0.9176853635075233 1101 32-33 -0.9299578492225251 1101 34-35 -0.9447141976939264 1101 36-37 -0.9492535896443073 1101 38-39 -0.9589927414602997 1101 40-41 -0.9643512363788602 1101 42-43 -1.0429140342050687 1101 44-45 -1.1006931048828932 1101 46-47 -1.2296881825067913 1101 48-49 -1.0851458344818 1101 50-51 -1.2115109963196815 1101 52-53 -1.0778089048001789 1101 54-55 -1.0219112229011955 1101 56-57 -0.8338449561915766 1101 58-59 -0.8015629085717038 1101 60-61 -0.7814836756556431 1101 62-63 -0.8287643385566739 1101 64-65 -0.8392860829436586 1101 66-67 -0.8814383204544711 1101 68-69 -0.9441309550299977 1101 70-71 -0.8924198594737547 1101 72-73 -0.9558256764509316 1101 74-75 -1.0099968331515257 1101 76-77 -1.0675536581126934 1101 78-79 -1.033863364591376 1101 80-81 -1.124723684712162 1101 82-83 -1.1245973625461154 1101 84-85 -1.2023639361399319 1101 86-87 -1.1813075035950114 1101 88-89 -1.2704225538923062 1101 90-91 -1.3789749814093888 1101 92-93 -1.5139793267476058 1101 94-95 -1.601333889924046 1101 96-97 -1.6008829355621614 1101 98-99 -1.7312572214584456 1101 100 -1.900481330736877 1102 1 -0.480442648596231 1102 2 -0.4161016014603902 1102 3 -0.483763558842341 1102 4 -0.3359681104153225 1102 5 -0.37671108277507415 1102 6 -0.3835428326178629 1102 7 -0.40337265440200554 1102 8 -0.4575852318691176 1102 9 -0.5286480908657509 1102 10-11 -0.5813911551477062 1102 12-13 -0.6069869970914183 1102 14-15 -0.7616355541061353 1102 16-17 -0.7860592455104083 1102 18-19 -0.7362037082744024 1102 20-21 -0.8075063715882251 1102 22-23 -0.8428057242218614 1102 24-25 -0.819328652978669 1102 26-27 -0.8648533710146253 1102 28-29 -0.9404549546527647 1102 30-31 -0.921374582948367 1102 32-33 -0.9517303389130163 1102 34-35 -0.9544768224743407 1102 36-37 -0.9939236800099067 1102 38-39 -1.0712245865438277 1102 40-41 -1.0507598584800988 1102 42-43 -1.0428559840808447 1102 44-45 -1.1063230368181678 1102 46-47 -1.187914196972379 1102 48-49 -1.2240087075044528 1102 50-51 -1.2090282182212917 1102 52-53 -1.042008145738734 1102 54-55 -0.9373566758092551 1102 56-57 -0.879460727398147 1102 58-59 -0.9306964494629497 1102 60-61 -0.8591062391097282 1102 62-63 -0.9143916092685274 1102 64-65 -0.9474679880688583 1102 66-67 -0.9308938256087345 1102 68-69 -1.0005608461277475 1102 70-71 -1.0081275993663041 1102 72-73 -1.0348530693252016 1102 74-75 -1.0806858536571724 1102 76-77 -1.0911394071737703 1102 78-79 -1.1000135875892099 1102 80-81 -1.2101729478093297 1102 82-83 -1.2226043830548257 1102 84-85 -1.1988639677638453 1102 86-87 -1.2349766489636842 1102 88-89 -1.3697982387579302 1102 90-91 -1.39555223118818 1102 92-93 -1.3504466008122549 1102 94-95 -1.4541728309759776 1102 96-97 -1.4134360205131848 1102 98-99 -1.514535946093389 1102 100 -1.6085819985157421 1103 1 -0.5176005553738818 1103 2 -0.45228805317794496 1103 3 -0.492385019391012 1103 4 -0.4446980130602469 1103 5 -0.34940039413821466 1103 6 -0.3466255000053877 1103 7 -0.42196006915479956 1103 8 -0.4179799828876014 1103 9 -0.5727670089891959 1103 10-11 -0.5338613518049442 1103 12-13 -0.5692020492347538 1103 14-15 -0.7226253471992408 1103 16-17 -0.7690505514012287 1103 18-19 -0.7245093185233955 1103 20-21 -0.7597157431235289 1103 22-23 -0.76269171138145 1103 24-25 -0.7274699899775854 1103 26-27 -0.8003237873072706 1103 28-29 -0.8559370442117498 1103 30-31 -0.8295605440637672 1103 32-33 -0.9113208172058123 1103 34-35 -0.9749898922581082 1103 36-37 -0.8644344740422198 1103 38-39 -0.9012580202410376 1103 40-41 -0.9205394761852475 1103 42-43 -1.038457607370205 1103 44-45 -1.0212847963461087 1103 46-47 -1.0545676615108661 1103 48-49 -1.1062046959593346 1103 50-51 -1.191516010638047 1103 52-53 -1.0229722879276792 1103 54-55 -0.87065007962903 1103 56-57 -0.8511907656649313 1103 58-59 -0.8126341147376692 1103 60-61 -0.900210760543203 1103 62-63 -0.8157867003110582 1103 64-65 -0.7698875374848413 1103 66-67 -0.8611373162440401 1103 68-69 -0.9145694997073193 1103 70-71 -0.8203242423882386 1103 72-73 -0.8373928448908714 1103 74-75 -0.8508350940224112 1103 76-77 -0.8776312619684532 1103 78-79 -0.9536127416189899 1103 80-81 -0.965625020529604 1103 82-83 -0.9167894087578929 1103 84-85 -0.9876850241796795 1103 86-87 -1.0481681277377533 1103 88-89 -1.0786127273457993 1103 90-91 -1.2142845508381122 1103 92-93 -1.3040040388866565 1103 94-95 -1.3137693425490262 1103 96-97 -1.3737309754445377 1103 98-99 -1.4454485722856525 1103 100 -1.4021332389757042 1104 1 -0.48066426993433 1104 2 -0.4647990128706567 1104 3 -0.5213312018376612 1104 4 -0.37865704669130196 1104 5 -0.37285961841602955 1104 6 -0.3965682363220395 1104 7 -0.3965454420555474 1104 8 -0.37600990785808364 1104 9 -0.5077475619051981 1104 10-11 -0.5521796518831792 1104 12-13 -0.517522711558918 1104 14-15 -0.7058828931633059 1104 16-17 -0.7948107434568783 1104 18-19 -0.7925255580702668 1104 20-21 -0.7009116915397371 1104 22-23 -0.7545391395494647 1104 24-25 -0.736572893301819 1104 26-27 -0.7503288233908378 1104 28-29 -0.8065973005407443 1104 30-31 -0.8069698201442819 1104 32-33 -0.9029691842446894 1104 34-35 -0.9710400238812227 1104 36-37 -0.9878466530801191 1104 38-39 -1.0309443792714887 1104 40-41 -0.8956154922563186 1104 42-43 -0.9131320648511974 1104 44-45 -1.0037528079280378 1104 46-47 -0.9957016862991637 1104 48-49 -1.0237948976956943 1104 50-51 -1.071361720268655 1104 52-53 -0.9259485227677118 1104 54-55 -0.7470313352563096 1104 56-57 -0.8247917688137747 1104 58-59 -0.8183673139426091 1104 60-61 -0.8763583874008489 1104 62-63 -0.8642852160246761 1104 64-65 -0.8192786218105184 1104 66-67 -0.8986875813036406 1104 68-69 -0.90023486479366 1104 70-71 -0.820498575859439 1104 72-73 -0.8060044057048188 1104 74-75 -0.8573324776968931 1104 76-77 -0.8660715532189371 1104 78-79 -0.9332957548696257 1104 80-81 -0.9433674443351023 1104 82-83 -0.9905700298990148 1104 84-85 -0.9185309501598837 1104 86-87 -0.956758892243105 1104 88-89 -1.086688297483171 1104 90-91 -1.0834160425855082 1104 92-93 -1.1462071343622107 1104 94-95 -1.1748949056024394 1104 96-97 -1.261006415224191 1104 98-99 -1.2516839310161743 1104 100 -1.1441942142688966 1105 1 -0.5726148442434962 1105 2 -0.5407640104480649 1105 3 -0.6415105441038449 1105 4 -0.42548267611549306 1105 5 -0.3801228096882312 1105 6 -0.41432995826938424 1105 7 -0.4146873271743985 1105 8 -0.4746667698707725 1105 9 -0.6648585516641035 1105 10-11 -0.668348152632781 1105 12-13 -0.6834718812796865 1105 14-15 -0.8517016661878216 1105 16-17 -0.8363189447266066 1105 18-19 -0.7853761513538657 1105 20-21 -0.8524391982782475 1105 22-23 -0.8532249906564573 1105 24-25 -0.9123193112196617 1105 26-27 -0.9108132183414952 1105 28-29 -0.9153563290788256 1105 30-31 -0.9463583561054492 1105 32-33 -0.9513893217719698 1105 34-35 -0.9322733896515913 1105 36-37 -0.9499782086386901 1105 38-39 -0.9484677890105857 1105 40-41 -0.9950000493327664 1105 42-43 -1.0185339566236422 1105 44-45 -1.0274506303573787 1105 46-47 -1.0975701460647471 1105 48-49 -1.0504610334127307 1105 50-51 -1.038003676733858 1105 52-53 -0.9256009369171636 1105 54-55 -0.8734531397172134 1105 56-57 -0.7629284615258385 1105 58-59 -0.7457005036929232 1105 60-61 -0.7159223525725977 1105 62-63 -0.7782044139626763 1105 64-65 -0.7825966933097348 1105 66-67 -0.7721876047620029 1105 68-69 -0.8172711795089853 1105 70-71 -0.8210137696656332 1105 72-73 -0.8003948608276588 1105 74-75 -0.8727550535725754 1105 76-77 -0.742286515298531 1105 78-79 -0.8543297722565555 1105 80-81 -0.9140342444945908 1105 82-83 -0.9768669565181334 1105 84-85 -1.0065630730440738 1105 86-87 -0.9505551181463048 1105 88-89 -0.9574304584961801 1105 90-91 -1.0391426000734967 1105 92-93 -0.9991425317406417 1105 94-95 -1.1073452438035964 1105 96-97 -1.0439845991028918 1105 98-99 -1.0557330856205276 1105 100 -1.163645559304868 1106 1 -0.5547175473101369 1106 2 -0.5102070392928368 1106 3 -0.5534931266423619 1106 4 -0.42555991309988883 1106 5 -0.4012273353445934 1106 6 -0.464822195828944 1106 7 -0.418236687741917 1106 8 -0.4058209223481626 1106 9 -0.5977958519482414 1106 10-11 -0.5777314251934556 1106 12-13 -0.6086975737041698 1106 14-15 -0.784830027825322 1106 16-17 -0.8100285621556864 1106 18-19 -0.8237940991210806 1106 20-21 -0.769248968828137 1106 22-23 -0.7313256376932316 1106 24-25 -0.735644581330213 1106 26-27 -0.8700545562781983 1106 28-29 -0.8846679809421474 1106 30-31 -0.8658587129628259 1106 32-33 -0.8912053660015928 1106 34-35 -0.9306462940391427 1106 36-37 -0.9660133641472939 1106 38-39 -0.9480389919996668 1106 40-41 -0.9765341795025577 1106 42-43 -0.9200222452433806 1106 44-45 -0.9195536235430453 1106 46-47 -0.929688328092773 1106 48-49 -0.8961327691476271 1106 50-51 -0.908705629997911 1106 52-53 -0.8441870620422236 1106 54-55 -0.7036788097720716 1106 56-57 -0.7286428022133506 1106 58-59 -0.7149990082515103 1106 60-61 -0.7305160804079733 1106 62-63 -0.7099043854420799 1106 64-65 -0.7216638626700487 1106 66-67 -0.7525831755969818 1106 68-69 -0.720424413706148 1106 70-71 -0.7032431918899036 1106 72-73 -0.784908570982509 1106 74-75 -0.7210946903386173 1106 76-77 -0.763857907509994 1106 78-79 -0.7691517609068868 1106 80-81 -0.7669486904264105 1106 82-83 -0.7518514535402687 1106 84-85 -0.7408294889842288 1106 86-87 -0.7967720415930089 1106 88-89 -0.8884214499025873 1106 90-91 -0.9639514625535952 1106 92-93 -0.938444362649669 1106 94-95 -0.9817034789829684 1106 96-97 -0.9576225755235441 1106 98-99 -0.9624264443350228 1106 100 -1.1840135065272506 1107 1 -0.5493436833578329 1107 2 -0.5509502044139296 1107 3 -0.48251219736950546 1107 4 -0.39791945481957214 1107 5 -0.3385994022887715 1107 6 -0.42906239346524444 1107 7 -0.40756787080541557 1107 8 -0.39644670470176635 1107 9 -0.5746284294658395 1107 10-11 -0.5802114320727583 1107 12-13 -0.6194973639307122 1107 14-15 -0.8191235860311394 1107 16-17 -0.7897845478228618 1107 18-19 -0.7788911098871338 1107 20-21 -0.806716342385748 1107 22-23 -0.8043088652210102 1107 24-25 -0.7806856917826579 1107 26-27 -0.86918399534445 1107 28-29 -0.8809087380896514 1107 30-31 -0.8985172602313583 1107 32-33 -0.9288989464970498 1107 34-35 -0.9834101533994826 1107 36-37 -1.0262638880944976 1107 38-39 -0.9542851869776499 1107 40-41 -0.987015758547777 1107 42-43 -1.026562618688999 1107 44-45 -0.9817792728073584 1107 46-47 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1.3088942906266254 2311 54-55 1.2038971570699175 2311 56-57 1.1497789272073717 2311 58-59 1.1320003579459765 2311 60-61 1.119999856237719 2311 62-63 1.0919363810204956 2311 64-65 1.0784281431953886 2311 66-67 1.0949867216951574 2311 68-69 1.1482563335282947 2311 70-71 1.1508745520889079 2311 72-73 1.1482218301238092 2311 74-75 1.1675410758247082 2311 76-77 1.1505067213907978 2311 78-79 1.1321689919634679 2311 80-81 1.0929276035423463 2311 82-83 1.2042814562504738 2311 84-85 1.2181712359757348 2311 86-87 1.1703865541412135 2311 88-89 1.2212550223359173 2311 90-91 1.2853241503830724 2311 92-93 1.3261115110270225 2311 94-95 1.3626951907928024 2311 96-97 1.3792008562712468 2311 98-99 1.4402476984376271 2311 100 1.553229032612684 2312 1 0.6147409918961984 2312 2 0.652859798445423 2312 3 0.6597168380981167 2312 4 0.34981409982315625 2312 5 0.4639696871578849 2312 6 0.5354789939494111 2312 7 0.4448077763269751 2312 8 0.5691458732970105 2312 9 0.7455079652366834 2312 10-11 0.6498927048089627 2312 12-13 0.7965294785513919 2312 14-15 1.030449849983249 2312 16-17 1.0498655183142418 2312 18-19 1.0042337578967775 2312 20-21 1.0122190797875064 2312 22-23 1.0737098025289527 2312 24-25 1.0671509914124755 2312 26-27 1.1124057128386582 2312 28-29 1.1394280148458407 2312 30-31 1.1344048374655884 2312 32-33 1.1304665731732229 2312 34-35 1.1274200735821367 2312 36-37 1.2356202173808128 2312 38-39 1.2287154703252199 2312 40-41 1.2860594221378605 2312 42-43 1.3001307062289982 2312 44-45 1.281215883724876 2312 46-47 1.325871858652384 2312 48-49 1.3265060327033922 2312 50-51 1.3748733000938174 2312 52-53 1.235502710986495 2312 54-55 1.1280454413510697 2312 56-57 1.0580794972120273 2312 58-59 1.07376137949678 2312 60-61 1.0351315570930026 2312 62-63 1.0176117001315035 2312 64-65 1.081484339046277 2312 66-67 1.0127261651250663 2312 68-69 0.9813227381872238 2312 70-71 0.9900056358542955 2312 72-73 0.9988539671573236 2312 74-75 1.0454633958009083 2312 76-77 1.004218633982589 2312 78-79 1.0245241572420696 2312 80-81 1.0428057832952469 2312 82-83 1.0899986712169287 2312 84-85 1.1193590444202464 2312 86-87 1.1380113439480475 2312 88-89 1.085460646408258 2312 90-91 1.1064631386350001 2312 92-93 1.171366266432834 2312 94-95 1.2128857444962513 2312 96-97 1.286628677334459 2312 98-99 0.9535659343714222 2312 100 1.2032574951428288 2313 1 0.631652985239981 2313 2 0.592852245512919 2313 3 0.6441484120475529 2313 4 0.5004558157625354 2313 5 0.358968130059111 2313 6 0.49452682391564196 2313 7 0.5997578157679655 2313 8 0.5705025797178962 2313 9 0.7370484211183737 2313 10-11 0.7387402947057424 2313 12-13 0.8276974195048794 2313 14-15 1.0025204029669936 2313 16-17 1.0090408438588554 2313 18-19 0.9688083323035457 2313 20-21 1.0452115828113975 2313 22-23 1.054770452411688 2313 24-25 1.0378552758069475 2313 26-27 1.0487484986312055 2313 28-29 1.150970679470369 2313 30-31 1.1697576496048612 2313 32-33 1.1659351760884746 2313 34-35 1.2128830631504002 2313 36-37 1.2122406979872054 2313 38-39 1.2420578826029427 2313 40-41 1.2549653282760858 2313 42-43 1.3144215734610043 2313 44-45 1.2620440430594044 2313 46-47 1.2786456548167067 2313 48-49 1.2622055737901405 2313 50-51 1.3186553348335295 2313 52-53 1.228192887759107 2313 54-55 1.051376506522324 2313 56-57 0.979980203655586 2313 58-59 1.024112499958612 2313 60-61 0.9905690310183886 2313 62-63 1.0639685552972367 2313 64-65 1.0725045303321465 2313 66-67 1.053483538442272 2313 68-69 0.9979024107644179 2313 70-71 1.0076092691005059 2313 72-73 1.0616223601062558 2313 74-75 1.0425369413891943 2313 76-77 0.8479930189875944 2313 78-79 1.0187683280379787 2313 80-81 1.088794884548225 2313 82-83 1.1374189457526889 2313 84-85 1.165416816208353 2313 86-87 1.2087510024629502 2313 88-89 1.2536097541399016 2313 90-91 1.2782777147322726 2313 92-93 1.3393261081588754 2313 94-95 1.3630071604243668 2313 96-97 1.4084275112693447 2313 98-99 1.4482718398485623 2313 100 1.5234721079777138 2314 1 0.6182164565418375 2314 2 0.5663470208704346 2314 3 0.6279373596037807 2314 4 0.4974351245087263 2314 5 0.5043510566998748 2314 6 0.48279012993311454 2314 7 0.5392533356172606 2314 8 0.548837812557899 2314 9 0.7090726310049362 2314 10-11 0.6568717599309792 2314 12-13 0.7637464225551795 2314 14-15 0.9615957836887503 2314 16-17 0.940949817382787 2314 18-19 0.9020804812014589 2314 20-21 0.9302067522834747 2314 22-23 0.9229558688050048 2314 24-25 0.9532729626598311 2314 26-27 0.9947666074678452 2314 28-29 1.000517102946148 2314 30-31 1.0614104787777734 2314 32-33 1.0060451493343905 2314 34-35 1.024673660454475 2314 36-37 1.0449409965888634 2314 38-39 1.0418971577135991 2314 40-41 1.1585510334034126 2314 42-43 1.2039288466661304 2314 44-45 1.1802631518431852 2314 46-47 1.2605215269953547 2314 48-49 1.1700065006503237 2314 50-51 1.2060188589804568 2314 52-53 1.158189098315077 2314 54-55 1.055777164462576 2314 56-57 1.0143833738193493 2314 58-59 1.0115152976513286 2314 60-61 0.9904381848707686 2314 62-63 0.9668781075388537 2314 64-65 1.0049734009669251 2314 66-67 1.0062026446648602 2314 68-69 1.009507718215147 2314 70-71 0.9568498801646896 2314 72-73 0.9680894837240288 2314 74-75 0.9920683298010715 2314 76-77 1.0098468130952654 2314 78-79 1.0361407308094996 2314 80-81 1.0615555057851331 2314 82-83 1.0628500076768255 2314 84-85 1.1315912435517 2314 86-87 1.171176210619219 2314 88-89 1.2185415680705347 2314 90-91 1.2680548732447967 2314 92-93 1.288577025925406 2314 94-95 1.2728626729537105 2314 96-97 1.332028885849077 2314 98-99 1.423488695736939 2314 100 1.5111594263825978 2315 1 0.46176193661722564 2315 2 0.4703210562329758 2315 3 0.5050469521161709 2315 4 0.21153384914907747 2315 5 0.3344404832597405 2315 6 0.34652501067942154 2315 7 0.41933143576360266 2315 8 0.4315832968018469 2315 9 0.5986478329058045 2315 10-11 0.47763847859493325 2315 12-13 0.5275081795369729 2315 14-15 0.7379308469740522 2315 16-17 0.7028636686266694 2315 18-19 0.5840430031138126 2315 20-21 0.7452438826255374 2315 22-23 0.5559299107494837 2315 24-25 0.7262911282525479 2315 26-27 0.8124480439971435 2315 28-29 0.8482113276270908 2315 30-31 0.8415099153641208 2315 32-33 0.8942552578705047 2315 34-35 0.9216605053599523 2315 36-37 1.0356762371476194 2315 38-39 1.003048409487981 2315 40-41 1.079576888341954 2315 42-43 1.156745064773915 2315 44-45 1.113334592716562 2315 46-47 1.1540378957197603 2315 48-49 1.179478933860139 2315 50-51 1.101253606442576 2315 52-53 1.097963198070886 2315 54-55 0.9863084719859501 2315 56-57 0.987225074986334 2315 58-59 0.9356135862384676 2315 60-61 0.8747743994462454 2315 62-63 0.8361872566482091 2315 64-65 0.8928748715906565 2315 66-67 0.9337607823501628 2315 68-69 0.9242359501098889 2315 70-71 0.8875423765391375 2315 72-73 0.6989463365058839 2315 74-75 0.9260679606642057 2315 76-77 0.9024235282927293 2315 78-79 0.9623275497163277 2315 80-81 0.6779598549085257 2315 82-83 0.7141781599301531 2315 84-85 0.9517090793894631 2315 86-87 1.0253238445232036 2315 88-89 1.0803365056847625 2315 90-91 1.132668771439807 2315 92-93 1.1584328321795638 2315 94-95 1.1702630416517792 2315 96-97 0.9665066531688886 2315 98-99 0.48412120874174747 2315 100 1.0175738239270977 2316 1 0.38702934173251435 2316 2 0.3713811851962703 2316 3 0.3688878261815063 2316 4 0.2926001486670273 2316 5 0.1995487857907463 2316 6 0.27186897477864136 2316 7 0.2570416324430056 2316 8 0.26565217437492805 2316 9 0.318884484703176 2316 10-11 0.33766046254716287 2316 12-13 0.1583620986823817 2316 14-15 0.17032852905946783 2316 16-17 0.3830813547248866 2316 18-19 0.44982242373030346 2316 20-21 0.4179120983450062 2316 22-23 0.4266744982174231 2316 24-25 0.5028457988478507 2316 26-27 0.5311648021338939 2316 28-29 0.4680471852520256 2316 30-31 0.3780147134351992 2316 32-33 0.5774633495562185 2316 34-35 0.5965306080497399 2316 36-37 0.5550786965482288 2316 38-39 0.7554410669171219 2316 40-41 0.8029076170987182 2316 42-43 0.7367575485799875 2316 44-45 0.6699383726245145 2316 46-47 0.5369568210314029 2316 48-49 0.4797298315525964 2316 50-51 0.5298343334696867 2316 52-53 0.6398014504072194 2316 54-55 0.4019747513533787 2316 56-57 0.2419487059188441 2316 58-59 0.24452144346904703 2316 60-61 0.3373144085961357 2316 62-63 0.4549619583254483 2316 64-65 0.3794746927440187 2316 66-67 0.3560160206191867 2316 68-69 0.33909600348713553 2316 70-71 0.11053415720467541 2316 72-73 -0.054922281272581586 2316 74-75 0.25241373182354465 2316 76-77 0.43191074741790203 2316 78-79 0.4028111123446507 2316 80-81 0.5946986411037862 2316 82-83 0.6450385220513404 2316 84-85 0.7254091225444235 2316 86-87 0.7948642009019231 2316 88-89 0.7548259539065896 2316 90-91 0.6221376410564616 2316 92-93 0.662699357763799 2316 94-95 0.8385018844933292 2316 96-97 0.7753625882306139 2316 98-99 0.6313081795745212 2316 100 0.48221176760597473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 34.0 1 711.0 2 266.0 3 236.0 4 198.0 5 158.0 6 166.0 7 768.0 8 2350.0 9 4715.0 10 7463.0 11 10995.0 12 15071.0 13 19323.0 14 23325.0 15 27900.0 16 34247.0 17 43132.0 18 54260.0 19 66909.0 20 85403.0 21 108104.0 22 118694.0 23 116192.0 24 111038.0 25 113224.0 26 125456.0 27 144998.0 28 173179.0 29 210069.0 30 257685.0 31 324276.0 32 413350.0 33 551569.0 34 816658.0 35 1130503.0 36 1718341.0 37 2246927.0 38 1647811.0 39 525286.0 40 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92637681365633 21.43401961512313 12.889164371921657 22.75043919929888 2 15.502119145805757 19.419203733733507 41.7874567464733 23.29122037398743 3 19.24415759547035 26.819595828590504 28.137395344743982 25.79885123119517 4 12.369886034580311 16.15753282489434 39.845245973919646 31.627335166605704 5 12.993705460944666 38.640307380597726 34.305068949417894 14.060918209039716 6 29.407953457024767 38.4846481865525 17.761725314172107 14.345673042250622 7 27.273750774022854 31.94052053998538 23.152707694909918 17.633020991081853 8 26.653082166809238 35.175569953208615 19.280958165194395 18.89038971478775 9 26.41679697909314 15.134214167387396 22.149798931934544 36.299189921584926 10-11 26.63790795127518 25.970126507613035 26.176616472952936 21.215349068158847 12-13 26.360167145829188 23.656313235181926 27.370769716928674 22.612749902060212 14-15 23.690691909059684 24.631716818866796 25.377759390336614 26.299831881736907 16-17 21.69237199686062 27.43703571218718 26.724919202019887 24.145673088932316 18-19 20.968835975682364 27.749529517909256 29.453243385149147 21.828391121259237 20-21 22.900794744192254 26.720821572437035 28.633090749544927 21.745292933825784 22-23 23.46399423174808 26.442955323206892 28.275289209371035 21.817761235673995 24-25 22.686221102391997 26.263981770774414 29.405698815503058 21.644098311330534 26-27 22.558419339679105 26.611180259720722 28.12983662768688 22.70056377291329 28-29 21.49631836126618 27.25306054290365 28.79242258420224 22.458198511627934 30-31 22.705188893930107 26.760035656733887 28.689323460765504 21.845451988570503 32-33 23.09452675637211 26.998928146714373 27.590394338353065 22.316150758560447 34-35 23.04697857047577 26.728087677958 28.678134334226186 21.54679941734004 36-37 22.435039278555085 26.291486909800447 28.279724012222147 22.993749799422318 38-39 22.85194555590423 26.527726538518635 28.805126158217153 21.81520174735998 40-41 22.427681261126935 25.339962125084654 29.49333010685946 22.739026506928948 42-43 22.35877550575878 25.67477141696919 28.676728906093196 23.289724171178836 44-45 22.565759113982466 26.765348683572913 28.428418749272012 22.24047345317261 46-47 22.54425794062472 26.584703746493993 27.704109020927415 23.166929291953867 48-49 23.457862303192073 26.459023295194502 27.617441242979424 22.465673158634008 50-51 22.943728120115193 26.167998842481722 27.77775826848779 23.110514768915298 52-53 22.32535011555486 26.94981182462869 27.33915734866448 23.38568071115197 54-55 22.202598970672113 26.797809577452767 27.35703149554843 23.642559956326696 56-57 23.406633832630884 26.2446566152165 26.8562170202768 23.492492531875815 58-59 22.30748458306384 26.829123422450458 27.796713223202662 23.066678771283037 60-61 22.326117001484747 27.614096198763228 27.03943084015644 23.02035595959558 62-63 22.87687346525947 26.617183066399292 26.547404504651112 23.95853896369012 64-65 21.92106094729761 26.918965781567493 27.119210018283752 24.040763252851143 66-67 22.649095117213676 26.382378129271 27.02823748174909 23.94028927176623 68-69 22.08473517529206 26.49524313617766 27.338048771141104 24.081972917389177 70-71 22.13097229937402 26.10692805732847 27.479944793026085 24.28215485027142 72-73 22.974562387498906 26.238953068879354 27.700445289484403 23.086039254137336 74-75 23.233842069129068 26.61890244376765 27.116452853018107 23.030802634085173 76-77 22.87164190117048 26.527614522779437 26.62778771568273 23.972955860367364 78-79 22.72134458257683 26.64312080811356 26.70545906201307 23.930075547296532 80-81 22.207138341651866 27.08855491880352 27.47522153687146 23.229085202673154 82-83 23.34308644195521 25.952726018158135 28.765022473427344 21.939165066459314 84-85 23.522144789123733 26.520902158782995 27.147057360774756 22.809895691318516 86-87 22.013746305017175 27.46703605087205 27.568140843402773 22.951076800708 88-89 23.2845769020533 25.93962922509241 27.395779505675534 23.380014367178756 90-91 22.937330373660213 26.11678915173718 27.47357762785815 23.472302846744462 92-93 23.394977346030903 26.378274058021805 27.31992000272021 22.906828593227075 94-95 22.014388297340364 26.438320787467024 28.175611013692663 23.37167990149995 96-97 22.43804750804524 26.367041561427445 27.60086407358654 23.594046856940775 98-99 21.79291399988478 27.009247827042465 27.327283570158027 23.870554602914726 100 22.858675051604244 26.339536629544565 26.196899282421416 24.60488903642978 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1394.0 1 989.5 2 851.0 3 1152.0 4 968.5 5 857.0 6 1278.5 7 2389.5 8 3843.0 9 4621.0 10 4383.5 11 4238.0 12 5023.5 13 6312.5 14 7748.0 15 9440.0 16 11153.0 17 12611.5 18 13938.0 19 15008.0 20 15885.5 21 16619.0 22 17627.5 23 19260.5 24 20963.0 25 22880.0 26 26330.5 27 30186.5 28 37625.0 29 56608.5 30 84719.5 31 111348.0 32 150919.0 33 195401.5 34 226283.0 35 258564.0 36 286281.5 37 339749.0 38 363130.5 39 333626.5 40 303690.0 41 272056.5 42 263207.0 43 281575.0 44 315409.5 45 384525.0 46 455887.0 47 511338.0 48 555351.0 49 551067.5 50 522176.5 51 539909.5 52 545765.5 53 513934.5 54 481218.5 55 405300.0 56 348807.0 57 283277.5 58 216655.0 59 210325.5 60 174206.5 61 115186.0 62 83177.0 63 72187.0 64 53802.5 65 23696.5 66 11628.0 67 7463.5 68 5530.5 69 4323.0 70 3435.5 71 2754.5 72 2245.0 73 2032.0 74 1714.5 75 1281.0 76 969.5 77 695.0 78 451.5 79 332.0 80 262.5 81 208.5 82 165.0 83 125.0 84 93.5 85 73.5 86 59.0 87 50.0 88 41.5 89 36.0 90 28.5 91 17.0 92 12.5 93 13.5 94 9.5 95 7.5 96 7.0 97 5.5 98 4.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057113293630143516 2 0.1329088379933343 3 0.016762476157244112 4 0.016495840799493677 5 0.01959769879465709 6 0.05307821188285358 7 0.05775321848874457 8 0.05031409200750739 9 0.041168499236667405 10-11 0.040239719407170055 12-13 0.041017405867275494 14-15 0.01863781150675552 16-17 0.01749127946842864 18-19 0.017380181402699292 20-21 0.020935319506038448 22-23 0.025352578599437348 24-25 0.024801532193419774 26-27 0.019548815645736174 28-29 0.018224526702242342 30-31 0.016291420358551675 32-33 0.022930640766537547 34-35 0.022792879165033156 36-37 0.022770659551887285 38-39 0.02089532420237588 40-41 0.02413049987641451 42-43 0.03104968741003834 44-45 0.040804097581075144 46-47 0.046750066058909884 48-49 0.03975977576321927 50-51 0.027716745438157886 52-53 0.03998197189467796 54-55 0.037115641798860774 56-57 0.038693234332217524 58-59 0.04375486220684664 60-61 0.023112841594333678 62-63 0.025601438266671085 64-65 0.02495262556281169 66-67 0.03193847193587313 68-69 0.038773224939542654 70-71 0.040470803383887095 72-73 0.044808071869960865 74-75 0.026783521686031358 76-77 0.020473151552604357 78-79 0.01963325017569048 80-81 0.024370471698389903 82-83 0.020068754593349527 84-85 0.012394100212766128 86-87 0.014282767330165053 88-89 0.018397839684780124 90-91 0.020717567297208923 92-93 0.01945104934789435 94-95 0.023072846290671113 96-97 0.024370471698389903 98-99 0.02717014295476949 100 0.028574422505588455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1251321E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.49752909428045 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94218004075277 50.395226757965695 2 14.148331914374804 18.53361562394601 3 4.5566528145387455 8.953485008783591 4 1.9270492051731265 5.048678455277475 5 0.8923817225916252 2.9224398919324543 6 0.503329665611651 1.978010965044814 7 0.2899672343440975 1.3294496157488396 8 0.17641578278302592 0.92438382924172 9 0.12335200386034992 0.7271326315512957 >10 0.4114380927255252 4.369929229661315 >50 0.01807451520149562 0.8204311126401943 >100 0.009266674993671674 1.1287231257080608 >500 7.804827113846076E-4 0.36447553004811584 >1k 6.139248688216464E-4 0.8331624209601962 >5k 8.296273445829673E-5 0.35326572365438513 >10k+ 8.296273445829673E-5 1.317590077835907 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39846 0.3541450821641299 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31225 0.27752296819191274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19378 0.17222866541626536 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 16651 0.14799151139675065 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 14139 0.12566524410778077 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.887845258347886E-6 0.0 10-11 0.0 0.0 0.0 8.887845258347886E-6 0.0 12-13 0.0 0.0 0.0 8.887845258347886E-6 0.0 14-15 0.0 0.0 0.0 1.7775690516695772E-5 0.0 16-17 0.0 0.0 0.0 4.4439226291739435E-5 0.0 18-19 0.0 0.0 0.0 7.11027620667831E-5 0.0 20-21 0.0 0.0 0.0 1.0665414310017464E-4 0.0 22-23 0.0 0.0 0.0 2.444157446045669E-4 0.0 24-25 0.0 0.0 0.0 5.954856323093084E-4 0.0 26-27 0.0 0.0 0.0 0.0014264991639648358 0.0 28-29 0.0 0.0 0.0 0.0040661892056941575 0.0 30-31 0.0 0.0 0.0 0.015691490803613192 0.0 32-33 0.0 0.0 0.0 0.044439226291739437 0.0 34-35 0.0 0.0 0.0 0.08683869209668801 0.0 36-37 0.0 4.443922629173943E-6 0.0 0.15907909835653966 0.0 38-39 0.0 8.887845258347886E-6 0.0 0.26356460721367736 0.0 40-41 0.0 8.887845258347886E-6 0.0 0.3732495055469487 0.0 42-43 0.0 8.887845258347886E-6 0.0 0.4826322171414361 0.0 44-45 0.0 8.887845258347886E-6 0.0 0.6074886673307072 0.0 46-47 0.0 8.887845258347886E-6 0.0 0.758470938656892 0.0 48-49 0.0 8.887845258347886E-6 0.0 0.9281399046387531 0.0 50-51 0.0 8.887845258347886E-6 0.0 1.1039414838488744 0.0 52-53 0.0 8.887845258347886E-6 0.0 1.27084188603276 0.0 54-55 0.0 8.887845258347886E-6 0.0 1.4489765246231976 0.0 56-57 0.0 8.887845258347886E-6 0.0 1.6606494472960107 0.0 58-59 0.0 8.887845258347886E-6 0.0 1.9083892460271998 0.0 60-61 0.0 8.887845258347886E-6 0.0 2.1545914475286945 0.0 62-63 0.0 8.887845258347886E-6 0.0 2.4068151641927202 0.0 64-65 0.0 8.887845258347886E-6 0.0 2.661749673660542 0.0 66-67 0.0 8.887845258347886E-6 0.0 2.9458985304925527 0.0 68-69 0.0 8.887845258347886E-6 0.0 3.2544134151003243 0.0 70-71 0.0 8.887845258347886E-6 0.0 3.5923115161321943 0.0 72-73 0.0 8.887845258347886E-6 0.0 3.9346313201800927 0.0 74-75 0.0 8.887845258347886E-6 0.0 4.256006916876694 0.0 76-77 0.0 8.887845258347886E-6 0.0 4.623674855601401 0.0 78-79 0.0 8.887845258347886E-6 0.0 5.017024223200103 0.0 80-81 0.0 1.7775690516695772E-5 0.0 5.434139689019627 0.0 82-83 0.0 2.2219613145869714E-5 0.0 5.85696559541764 8.887845258347886E-6 84-85 0.0 2.666353577504366E-5 0.0 6.257505229830347 8.887845258347886E-6 86-87 0.0 2.666353577504366E-5 0.0 6.6797889776676005 8.887845258347886E-6 88 0.0 2.666353577504366E-5 0.0 7.0230686690034 8.887845258347886E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 13125 0.0 39.13824 94 GTACTAG 13990 0.0 38.681686 1 GGTATCA 29450 0.0 35.824123 1 GCATCGT 14335 0.0 35.315556 8 GTATCAA 46880 0.0 34.57011 1 ACCTGGG 4290 0.0 34.516167 3 TACCTGG 4495 0.0 34.23302 2 TAGGCAT 17180 0.0 33.928894 5 TATTCTC 5435 0.0 31.136812 5 CTAGGCA 19220 0.0 31.012243 4 CTGTGCG 18690 0.0 30.95929 9 CAACGCA 50345 0.0 30.280413 5 ACTGTGC 20040 0.0 29.859238 8 TAGTACT 19490 0.0 29.786703 4 ATCAACG 51420 0.0 29.711226 3 TATCAAC 56685 0.0 28.4494 2 TACTGTG 22300 0.0 28.204638 7 GTACTGT 20990 0.0 28.01664 6 CATCGTC 18460 0.0 27.729269 9 AAGCTAA 7355 0.0 27.67244 9 >>END_MODULE