FastQCFastQC Report
Mon 6 Jun 2016
ERR1211209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251321
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355460.315927347553234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297920.26478668593670024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183840.16339414722946755No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC168350.14962687492428667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC134700.042.82306394
GTACTAG139200.037.4916651
GGTATCA319400.034.224651
TAGGCAT169250.033.6743555
ACCTGGG40350.033.6647873
TACCCCG6950.033.126725
TATTCTC55950.032.7515345
GTATCAA490650.032.5709461
CTGTGCG198450.032.3180279
TACCTGG44700.031.9632552
ACTGTGC210300.031.1448888
TAGTACT205900.030.9545824
CTAGGCA193950.030.3172024
TACTGTG229400.029.8014037
GTACTGT221000.029.1269826
AAGCTAA72450.029.0537979
CAACGCA526600.028.355695
CTAACGC71200.028.2543543
GCATCGT176700.027.973228
ATCAACG537900.027.8135663
Produced by FastQC (version 0.11.5)