##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211209_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11251321 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13752082977634 33.0 31.0 34.0 30.0 34.0 2 32.285830881547156 34.0 31.0 34.0 30.0 34.0 3 32.36221409023883 34.0 31.0 34.0 30.0 34.0 4 35.97697061527264 37.0 35.0 37.0 35.0 37.0 5 35.86651398533559 37.0 35.0 37.0 35.0 37.0 6 35.8928392497201 37.0 35.0 37.0 35.0 37.0 7 35.830825998120574 37.0 35.0 37.0 35.0 37.0 8 35.82701115717879 37.0 35.0 37.0 35.0 37.0 9 37.41735748184591 39.0 37.0 39.0 34.0 39.0 10-11 37.39547471803533 39.0 37.0 39.0 34.0 39.0 12-13 37.27319520970026 39.0 37.0 39.0 33.5 39.0 14-15 38.462770371585705 40.0 38.0 41.0 34.0 41.0 16-17 38.4272522755328 40.0 38.0 41.0 34.0 41.0 18-19 38.457066152498896 40.0 38.0 41.0 34.0 41.0 20-21 38.41734401676034 40.0 38.0 41.0 34.0 41.0 22-23 38.271891629436226 40.0 38.0 41.0 34.0 41.0 24-25 38.19549233374463 40.0 37.5 41.0 33.5 41.0 26-27 38.03982990086231 40.0 37.0 41.0 33.0 41.0 28-29 38.028061949347986 40.0 37.0 41.0 33.0 41.0 30-31 38.03835709602455 40.0 37.0 41.0 33.0 41.0 32-33 37.90288260374048 40.0 37.0 41.0 33.0 41.0 34-35 37.760236686874364 40.0 37.0 41.0 33.0 41.0 36-37 37.624145600325505 40.0 36.0 41.0 33.0 41.0 38-39 37.514809994310895 40.0 36.0 41.0 32.0 41.0 40-41 37.39807507936179 39.5 36.0 41.0 32.0 41.0 42-43 37.233171242736745 39.0 36.0 41.0 32.0 41.0 44-45 37.028852300987595 39.0 35.0 41.0 31.0 41.0 46-47 36.73755508353197 39.0 35.0 41.0 31.0 41.0 48-49 36.42729209307956 39.0 35.0 40.5 30.0 41.0 50-51 36.17568963679909 38.5 35.0 40.0 29.5 41.0 52-53 35.9303623103456 38.0 35.0 40.0 29.0 41.0 54-55 35.65549476368153 38.0 34.5 40.0 29.0 41.0 56-57 35.338575177083655 37.0 34.0 40.0 28.0 41.0 58-59 35.00492546608527 37.0 34.0 40.0 28.0 41.0 60-61 34.724428535991464 36.0 34.0 39.0 27.0 41.0 62-63 34.393297284825486 35.5 33.5 39.0 26.5 41.0 64-65 34.009522304092116 35.0 33.0 39.0 26.0 40.0 66-67 33.717949563433486 35.0 33.0 38.0 26.0 40.0 68-69 33.42227948167153 35.0 33.0 37.5 26.0 40.0 70-71 33.17081607573013 35.0 32.0 37.0 25.5 40.0 72-73 32.89060133472327 35.0 32.0 36.5 25.0 39.0 74-75 32.59934264607685 35.0 32.0 36.0 25.0 39.0 76-77 31.662637613841078 34.0 30.5 35.0 23.5 38.0 78-79 32.199634736223416 34.5 31.5 35.0 24.5 38.0 80-81 32.1763427601079 35.0 32.0 35.0 25.0 37.0 82-83 31.969370707670684 34.5 31.5 35.0 24.0 37.0 84-85 31.756890635330734 34.0 31.0 35.0 24.0 36.0 86-87 31.556691209858826 34.0 31.0 35.0 24.0 36.0 88-89 31.3701601794136 34.0 31.0 35.0 23.5 36.0 90-91 31.171763475595444 34.0 31.0 35.0 23.0 35.0 92-93 30.983879937298028 34.0 31.0 35.0 22.0 35.0 94-95 30.812173699426047 34.0 30.0 35.0 20.0 35.0 96-97 30.656041721678726 34.0 30.0 35.0 20.0 35.0 98-99 30.493601062488572 34.0 30.0 35.0 20.0 35.0 100 28.610781613998924 32.0 25.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34801266694896427 1101 2 -0.3144522760805337 1101 3 -0.3734937519157775 1101 4 -0.17948250617038042 1101 5 -0.23424012780468217 1101 6 -0.32451998542058647 1101 7 -0.3419731043663674 1101 8 -0.308595864438864 1101 9 -0.40255953399339717 1101 10-11 -0.4505371540067955 1101 12-13 -0.45819684446704656 1101 14-15 -0.6822111921657878 1101 16-17 -0.6348231560605129 1101 18-19 -0.692104697514047 1101 20-21 -0.6740783947240345 1101 22-23 -0.7114665617933866 1101 24-25 -0.6753610262727889 1101 26-27 -0.7950807650916545 1101 28-29 -0.8310177904817166 1101 30-31 -0.6239262974715132 1101 32-33 -0.6167251508526661 1101 34-35 -0.6625707184166103 1101 36-37 -0.5976002274196262 1101 38-39 -0.6436442346049276 1101 40-41 -0.6669427886792292 1101 42-43 -0.6185317343701868 1101 44-45 -0.6987018046987004 1101 46-47 -0.6491575109373713 1101 48-49 -0.642375547295778 1101 50-51 -0.7443157217582197 1101 52-53 -0.7700751399351802 1101 54-55 -0.6764657746995724 1101 56-57 -0.6967111164563065 1101 58-59 -0.6602837602153784 1101 60-61 -0.6635396781860265 1101 62-63 -0.8131713484167733 1101 64-65 -0.8250711941390918 1101 66-67 -0.8142185870294654 1101 68-69 -0.9249765894369588 1101 70-71 -1.0254792016243854 1101 72-73 -1.028139875719262 1101 74-75 -1.1050577428476487 1101 76-77 -0.9879749548527883 1101 78-79 -0.8805380786192138 1101 80-81 -0.8383855515409451 1101 82-83 -0.8399319327884527 1101 84-85 -0.7367164116854461 1101 86-87 -0.8744479381223158 1101 88-89 -0.876961166450922 1101 90-91 -0.9037228795804673 1101 92-93 -1.1049211308202693 1101 94-95 -1.0235668569410485 1101 96-97 -1.042064650881759 1101 98-99 -1.1713093494268882 1101 100 -0.9902088201981698 1102 1 -0.4375239888569844 1102 2 -0.3861101482354492 1102 3 -0.42099932549367836 1102 4 -0.2958070217937987 1102 5 -0.3253371865181478 1102 6 -0.32827475061905176 1102 7 -0.3053978056407587 1102 8 -0.36408560067354045 1102 9 -0.5028045481935166 1102 10-11 -0.5334340445116794 1102 12-13 -0.5180507080666601 1102 14-15 -0.612997799252561 1102 16-17 -0.6824338873563889 1102 18-19 -0.6534278037395396 1102 20-21 -0.7624490672541455 1102 22-23 -0.719701746099517 1102 24-25 -0.7967284891398592 1102 26-27 -0.8721078633929977 1102 28-29 -0.8300216919315275 1102 30-31 -0.8058867918725454 1102 32-33 -0.6833662010554633 1102 34-35 -0.7571323464200361 1102 36-37 -0.78071031671724 1102 38-39 -0.8371584271763979 1102 40-41 -0.7857808856243551 1102 42-43 -0.7809266286292385 1102 44-45 -0.8202824007033129 1102 46-47 -0.8476863095985294 1102 48-49 -0.7787070847410149 1102 50-51 -0.8004589459135403 1102 52-53 -0.9231430315524776 1102 54-55 -0.8427495348681902 1102 56-57 -0.8701145520169433 1102 58-59 -0.9101992291673753 1102 60-61 -0.857935674576737 1102 62-63 -0.9541683431601982 1102 64-65 -0.9067481292763588 1102 66-67 -1.0277866628095538 1102 68-69 -0.9552214154255481 1102 70-71 -0.9603551417792531 1102 72-73 -1.0092404568332896 1102 74-75 -1.121790650122616 1102 76-77 -0.8892405843426445 1102 78-79 -0.7588046975326783 1102 80-81 -0.7282166470173657 1102 82-83 -0.7811706808579189 1102 84-85 -0.8526916417357349 1102 86-87 -0.9127088516003852 1102 88-89 -0.9814591319062309 1102 90-91 -0.9358531582845302 1102 92-93 -0.8936204841386051 1102 94-95 -1.0121705123590168 1102 96-97 -1.203049271811711 1102 98-99 -1.2315264503259904 1102 100 -1.0959708394189427 1103 1 -0.31278404077735544 1103 2 -0.3338836370141465 1103 3 -0.3789915199892775 1103 4 -0.2566968156901126 1103 5 -0.2486921482610569 1103 6 -0.3127671800294394 1103 7 -0.3867011086562826 1103 8 -0.38877386657585333 1103 9 -0.5052497485136556 1103 10-11 -0.5089957789094157 1103 12-13 -0.5639066754332092 1103 14-15 -0.6638415398789306 1103 16-17 -0.6631180336407709 1103 18-19 -0.6662583427916573 1103 20-21 -0.7295190866335801 1103 22-23 -0.7962281175352075 1103 24-25 -0.8605636039757556 1103 26-27 -0.8181751202040317 1103 28-29 -0.8752652282665068 1103 30-31 -0.826556685397847 1103 32-33 -0.748577785470566 1103 34-35 -0.8689345874028191 1103 36-37 -0.8599780869895 1103 38-39 -0.8554167719600372 1103 40-41 -0.8850959536748704 1103 42-43 -0.7787496623176509 1103 44-45 -0.8031254919326969 1103 46-47 -0.8967166086673544 1103 48-49 -0.9930290257455994 1103 50-51 -1.0679544154140004 1103 52-53 -0.9884503452452975 1103 54-55 -0.9711781252492742 1103 56-57 -0.9455932468748642 1103 58-59 -0.9457445606132637 1103 60-61 -1.0343978910552636 1103 62-63 -0.8873316136947906 1103 64-65 -0.9365166294970564 1103 66-67 -1.0373324939052182 1103 68-69 -1.0867943049753492 1103 70-71 -1.1221250190834766 1103 72-73 -1.0649053330882623 1103 74-75 -0.9934886709204918 1103 76-77 -0.9762161032578334 1103 78-79 -0.8822760041842344 1103 80-81 -0.8767146141204343 1103 82-83 -0.8950182223123662 1103 84-85 -0.9665324667228781 1103 86-87 -0.9298613011906411 1103 88-89 -0.9362256910447933 1103 90-91 -0.951620270712283 1103 92-93 -1.1299805069127409 1103 94-95 -1.0797194597108657 1103 96-97 -1.1837047427043892 1103 98-99 -1.1851322469650256 1103 100 -1.2482634259069592 1104 1 -0.36343254392282986 1104 2 -0.33878101351876566 1104 3 -0.44313156332699677 1104 4 -0.29852718061322747 1104 5 -0.32768594944353424 1104 6 -0.3876563571677494 1104 7 -0.3192492866086738 1104 8 -0.39567913819210787 1104 9 -0.5625877680748346 1104 10-11 -0.5326240939305862 1104 12-13 -0.5601117484712859 1104 14-15 -0.7035830097772973 1104 16-17 -0.7014593144201413 1104 18-19 -0.7740116175113911 1104 20-21 -0.7508191707633358 1104 22-23 -0.7638456868681942 1104 24-25 -0.7370297602171192 1104 26-27 -0.8257880393442179 1104 28-29 -0.8549029933858989 1104 30-31 -0.8305342162457734 1104 32-33 -0.7931977366922993 1104 34-35 -0.8672820090000073 1104 36-37 -0.8396189587385905 1104 38-39 -0.8130524521547571 1104 40-41 -0.8901019043335126 1104 42-43 -0.7686542767122333 1104 44-45 -0.8962335709298799 1104 46-47 -0.9746585533274583 1104 48-49 -0.9365028302863863 1104 50-51 -0.8595841194562084 1104 52-53 -0.8711282516889369 1104 54-55 -0.9315745126243513 1104 56-57 -0.8611699624948699 1104 58-59 -0.9020189426064036 1104 60-61 -0.8662090779963165 1104 62-63 -0.9357377013702006 1104 64-65 -0.9486256683917347 1104 66-67 -0.9998324782327543 1104 68-69 -1.0285357524911163 1104 70-71 -1.057390007399249 1104 72-73 -0.9672817887172549 1104 74-75 -0.9635808077992465 1104 76-77 -0.8556964147250881 1104 78-79 -0.711114587652105 1104 80-81 -0.7406578067262259 1104 82-83 -0.7738400722374479 1104 84-85 -0.8281125682441903 1104 86-87 -0.9421337655248863 1104 88-89 -1.026307021124385 1104 90-91 -0.9542885684291988 1104 92-93 -0.9786551021256216 1104 94-95 -0.9553181697119051 1104 96-97 -1.0171304669264103 1104 98-99 -1.125535261816804 1104 100 -1.1290619885543762 1105 1 -0.43973586001351705 1105 2 -0.3802532515844952 1105 3 -0.46490848392328843 1105 4 -0.2338309144220645 1105 5 -0.3173331061664584 1105 6 -0.35239099580081046 1105 7 -0.40837438872812015 1105 8 -0.4575732151980958 1105 9 -0.6187020482915386 1105 10-11 -0.5726437611897381 1105 12-13 -0.6008730185448812 1105 14-15 -0.8237755178675954 1105 16-17 -0.7620042451826166 1105 18-19 -0.6963612865298572 1105 20-21 -0.8464898592925039 1105 22-23 -0.8230235032747188 1105 24-25 -0.8044750801618861 1105 26-27 -0.8469247042947359 1105 28-29 -0.8555462302274961 1105 30-31 -0.8129710203574447 1105 32-33 -0.8040689573150033 1105 34-35 -0.8652149206546582 1105 36-37 -0.842795483599339 1105 38-39 -0.8904319664507696 1105 40-41 -0.9563142637347184 1105 42-43 -0.8839198663227634 1105 44-45 -0.8728220377273033 1105 46-47 -0.882975207477152 1105 48-49 -0.9685284411597195 1105 50-51 -1.0083793804501866 1105 52-53 -1.0987370900901254 1105 54-55 -1.0200877141823597 1105 56-57 -0.9992048736739818 1105 58-59 -1.065530424918208 1105 60-61 -0.9419877305999336 1105 62-63 -0.9807519508117934 1105 64-65 -1.0537294001123954 1105 66-67 -1.0885830018642935 1105 68-69 -0.982774356020208 1105 70-71 -1.0346748869385678 1105 72-73 -1.036968994905905 1105 74-75 -1.0843117117628438 1105 76-77 -1.0598264204395456 1105 78-79 -0.9861418952710181 1105 80-81 -0.8538211676514038 1105 82-83 -0.844199018737342 1105 84-85 -0.9127730395812357 1105 86-87 -0.9395233441221968 1105 88-89 -0.953365077525536 1105 90-91 -0.9262891367179691 1105 92-93 -0.9714403114445531 1105 94-95 -0.9638365388558761 1105 96-97 -1.0402786431778388 1105 98-99 -1.171010517446252 1105 100 -1.1587111277050788 1106 1 -0.4098513776126147 1106 2 -0.37861697481665857 1106 3 -0.4180235209458516 1106 4 -0.29664604660201377 1106 5 -0.32145422352925834 1106 6 -0.3779249280199508 1106 7 -0.42930056117744897 1106 8 -0.42918845874862654 1106 9 -0.5770343969493865 1106 10-11 -0.5291537534295401 1106 12-13 -0.628836594637022 1106 14-15 -0.7560497575794116 1106 16-17 -0.6922746050946103 1106 18-19 -0.6842562830965946 1106 20-21 -0.731206866851764 1106 22-23 -0.699109895394372 1106 24-25 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-0.759550955766727 1106 90-91 -0.827127237596109 1106 92-93 -0.837294559765521 1106 94-95 -0.8341270006948918 1106 96-97 -0.9173695415293253 1106 98-99 -0.9151366296869625 1106 100 -0.9196399620032842 1107 1 -0.38162216784218117 1107 2 -0.37972664679348966 1107 3 -0.4242401378229417 1107 4 -0.2783368643488089 1107 5 -0.30777608818030444 1107 6 -0.3261079597404333 1107 7 -0.36556930685625844 1107 8 -0.3840856788341256 1107 9 -0.46454478509190267 1107 10-11 -0.5039051352362591 1107 12-13 -0.5328734662733083 1107 14-15 -0.7121043340264137 1107 16-17 -0.7222404642102092 1107 18-19 -0.7314387183366406 1107 20-21 -0.6772148474530653 1107 22-23 -0.665367889724024 1107 24-25 -0.7794760986935287 1107 26-27 -0.7328969493873814 1107 28-29 -0.7615796393417114 1107 30-31 -0.7716198990471881 1107 32-33 -0.7462956089531403 1107 34-35 -0.8283021240890918 1107 36-37 -0.7809178383423685 1107 38-39 -0.777373319133126 1107 40-41 -0.8277557056648988 1107 42-43 -0.7198994538849917 1107 44-45 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1.009770428104531 2311 36-37 1.0422473004372605 2311 38-39 1.0727043992250103 2311 40-41 1.0786901440629393 2311 42-43 1.0441760446572559 2311 44-45 1.0322543520190166 2311 46-47 0.6902919852472138 2311 48-49 0.9598644654895025 2311 50-51 1.0577456430138596 2311 52-53 0.9931590483243511 2311 54-55 1.0420198053695344 2311 56-57 1.1265453469723283 2311 58-59 1.1509806171189112 2311 60-61 1.1713169279708495 2311 62-63 1.1403062337689747 2311 64-65 1.1994024697131067 2311 66-67 1.1938709247960801 2311 68-69 1.255595771659273 2311 70-71 1.237319285010706 2311 72-73 1.2713779364305964 2311 74-75 1.2741287254310123 2311 76-77 1.2461770741071803 2311 78-79 0.9131466915343793 2311 80-81 0.864819907052528 2311 82-83 1.0115854532876796 2311 84-85 1.1148007936032194 2311 86-87 1.1168056453543969 2311 88-89 1.148338996122387 2311 90-91 1.1841105921206605 2311 92-93 1.257451869066518 2311 94-95 1.3071771114584756 2311 96-97 1.313043458788254 2311 98-99 1.3878602666910211 2311 100 1.5189834989781374 2312 1 0.3230496805329821 2312 2 0.3907698696756938 2312 3 0.4727288881599918 2312 4 0.1861242635387299 2312 5 0.3569641754847339 2312 6 0.43385261952297327 2312 7 0.34320757514537803 2312 8 0.40711747598714254 2312 9 0.651064472475511 2312 10-11 0.6393635545981624 2312 12-13 0.7164545127441428 2312 14-15 0.9069646699883052 2312 16-17 0.8852158114920101 2312 18-19 0.85144885788376 2312 20-21 0.8691636520599104 2312 22-23 0.9424826659903829 2312 24-25 0.9589531189126532 2312 26-27 0.9976227383153002 2312 28-29 1.0109269685570794 2312 30-31 1.0340783675562548 2312 32-33 0.9371371043884196 2312 34-35 1.0150814775121475 2312 36-37 1.0671157887140978 2312 38-39 0.8852286325507563 2312 40-41 0.9873672291487878 2312 42-43 1.0601227380776592 2312 44-45 0.8654489802217213 2312 46-47 0.9263454938149565 2312 48-49 1.0551661763440734 2312 50-51 0.9503878039262261 2312 52-53 1.1139312747790413 2312 54-55 0.9721743046805642 2312 56-57 1.0210623202205156 2312 58-59 1.0796620151588385 2312 60-61 1.1061696577098274 2312 62-63 1.1425188354568547 2312 64-65 1.1235530501571205 2312 66-67 1.1664139161962552 2312 68-69 1.2387495232185302 2312 70-71 1.1740300462452495 2312 72-73 1.1568263829079939 2312 74-75 1.1319307252374315 2312 76-77 1.1576397354087575 2312 78-79 1.047740671842142 2312 80-81 1.0052116707565375 2312 82-83 1.0594308706596252 2312 84-85 1.0945679490919566 2312 86-87 1.1041827666472983 2312 88-89 1.139128784050893 2312 90-91 1.224702495930785 2312 92-93 1.2785656613349197 2312 94-95 1.2539333101401589 2312 96-97 0.8796293538832174 2312 98-99 0.6871230284427696 2312 100 0.9253540869305681 2313 1 0.06922762280629513 2313 2 0.24700747279659652 2313 3 0.2941082793497145 2313 4 0.18542827934251704 2313 5 0.33617912618290546 2313 6 0.3185421477208621 2313 7 0.2921648029759609 2313 8 0.3967168707136892 2313 9 0.5951520421052123 2313 10-11 0.3825184704616973 2313 12-13 0.4795482850337365 2313 14-15 0.5394100989294728 2313 16-17 0.8020309908322574 2313 18-19 0.694177070837263 2313 20-21 0.7008950172235373 2313 22-23 0.6794931095309735 2313 24-25 0.5447008781188387 2313 26-27 0.7648461337067118 2313 28-29 0.627420197951615 2313 30-31 0.8713854626143629 2313 32-33 0.9526578376485446 2313 34-35 1.0022452758037517 2313 36-37 1.0248186229644105 2313 38-39 1.0234600292341227 2313 40-41 1.0482616791009818 2313 42-43 1.0850857938727998 2313 44-45 0.925690475735415 2313 46-47 0.9038154550303332 2313 48-49 1.013967703741102 2313 50-51 0.9240616315158476 2313 52-53 0.9507116360212322 2313 54-55 0.88015993710939 2313 56-57 0.5931501854510799 2313 58-59 0.6930402914705596 2313 60-61 0.5881055411278737 2313 62-63 0.8903324220467681 2313 64-65 1.0585584545530224 2313 66-67 0.9813075128724975 2313 68-69 1.0723747879784398 2313 70-71 1.0996036996145193 2313 72-73 1.0601679947968208 2313 74-75 1.1272521510346536 2313 76-77 1.1007736499173006 2313 78-79 0.9535946962518764 2313 80-81 0.8535807012778065 2313 82-83 0.9386582462741089 2313 84-85 0.9213268804523516 2313 86-87 0.8920284774009133 2313 88-89 0.9423700344897448 2313 90-91 0.8780193465255657 2313 92-93 1.02260236849245 2313 94-95 1.1271236815796364 2313 96-97 1.2323127875563564 2313 98-99 1.2132458926262153 2313 100 1.2552795421547138 2314 1 -0.25562546165310707 2314 2 0.11765663574432494 2314 3 0.34043931002727135 2314 4 0.2564301330291059 2314 5 0.33708554183211703 2314 6 0.4259682759374783 2314 7 0.47176078587258274 2314 8 0.49614586476771905 2314 9 0.5021481314687719 2314 10-11 0.607260838084045 2314 12-13 0.47902801328128675 2314 14-15 0.8398587444480938 2314 16-17 0.9019370287191535 2314 18-19 0.8687212750790678 2314 20-21 0.8952388920448939 2314 22-23 0.9717894067904496 2314 24-25 0.8578439866529166 2314 26-27 0.763785691680333 2314 28-29 0.8084995551128458 2314 30-31 0.8613205484023112 2314 32-33 0.914611211613142 2314 34-35 0.958528649100181 2314 36-37 0.9085251422322855 2314 38-39 0.6686613361307394 2314 40-41 0.708603889201342 2314 42-43 0.9105782006589251 2314 44-45 0.9720645907930034 2314 46-47 0.9262078776283218 2314 48-49 0.9501030078330075 2314 50-51 0.930550919328347 2314 52-53 0.9753978487212862 2314 54-55 0.8580646667036831 2314 56-57 0.8461424305441199 2314 58-59 0.9591428449040009 2314 60-61 1.049314336060462 2314 62-63 0.9430583872080973 2314 64-65 0.9711757326953503 2314 66-67 0.7329493097647131 2314 68-69 0.6959319608212837 2314 70-71 0.8651540761131216 2314 72-73 1.0215007431642817 2314 74-75 1.0792180602234254 2314 76-77 0.957039131431408 2314 78-79 0.8640154365568051 2314 80-81 0.9081154645577243 2314 82-83 0.911322192219032 2314 84-85 0.9796089715747449 2314 86-87 1.0112962664117333 2314 88-89 0.9490735789990232 2314 90-91 1.038386282016475 2314 92-93 0.950750310368953 2314 94-95 0.9800922933084841 2314 96-97 0.9989344137648537 2314 98-99 1.1679188957476647 2314 100 1.2245139737006738 2315 1 0.4221441618382542 2315 2 0.44222488821858263 2315 3 0.468247766936436 2315 4 0.3095164847282348 2315 5 0.3883367402928215 2315 6 0.4335873596004518 2315 7 0.3767651409197157 2315 8 0.2889774242695111 2315 9 0.536297569919995 2315 10-11 0.591078656699068 2315 12-13 0.44951905855842256 2315 14-15 0.644318581479375 2315 16-17 0.7842069955681339 2315 18-19 0.7861433833606739 2315 20-21 0.5011050418102485 2315 22-23 0.8827155563802904 2315 24-25 0.8850991084955098 2315 26-27 0.855264087574966 2315 28-29 0.819388599042874 2315 30-31 0.8609598035027659 2315 32-33 0.8436036489695695 2315 34-35 0.8999159948909252 2315 36-37 0.8784184380924742 2315 38-39 0.9182007515013098 2315 40-41 0.9155871579220118 2315 42-43 1.009133822399491 2315 44-45 0.976685027043672 2315 46-47 0.9553058727708574 2315 48-49 0.886341004028651 2315 50-51 0.9333646966351452 2315 52-53 0.9805500504883184 2315 54-55 0.8086210870523871 2315 56-57 0.8006461060064538 2315 58-59 0.7772362557286527 2315 60-61 0.8856405987498803 2315 62-63 0.9528099081836245 2315 64-65 1.0256399302587127 2315 66-67 1.0741857098410605 2315 68-69 1.0683521164621084 2315 70-71 1.0139750732543646 2315 72-73 1.0735741076082306 2315 74-75 0.47967682110830623 2315 76-77 0.45972074896393167 2315 78-79 0.7921089598228477 2315 80-81 0.8352636299529905 2315 82-83 0.7625190897141607 2315 84-85 0.7729739969261651 2315 86-87 0.8698124541329122 2315 88-89 0.8742160230470937 2315 90-91 0.9295571781250089 2315 92-93 0.9299130690115796 2315 94-95 1.0262723712166597 2315 96-97 1.1442642016290812 2315 98-99 1.144123773839258 2315 100 1.0911130109829514 2316 1 0.31269962418446795 2316 2 0.12686528801171448 2316 3 0.21058399859990118 2316 4 0.05748614739304969 2316 5 0.3004302706803159 2316 6 0.36934738858952443 2316 7 0.41621336274488385 2316 8 0.4434283097678815 2316 9 0.6032680654151008 2316 10-11 0.6095453927826782 2316 12-13 0.3842034997664783 2316 14-15 0.48870850086002093 2316 16-17 0.7812219086787735 2316 18-19 0.7480033018246033 2316 20-21 0.7463491743781461 2316 22-23 0.7916010469718202 2316 24-25 0.4790645157694158 2316 26-27 0.7566831205578097 2316 28-29 0.8994989598668113 2316 30-31 0.5607733463783902 2316 32-33 0.713284475192566 2316 34-35 0.8293920685328473 2316 36-37 0.8576471922717559 2316 38-39 0.7996397598711269 2316 40-41 0.8006693772114986 2316 42-43 0.7831758729781484 2316 44-45 0.6394158066523872 2316 46-47 0.82696073596356 2316 48-49 0.8893711917484239 2316 50-51 0.9446224346922989 2316 52-53 0.9259248370917774 2316 54-55 0.8008696899211998 2316 56-57 0.8760293657716716 2316 58-59 0.7250330607045328 2316 60-61 0.8374936137166173 2316 62-63 0.8889383975522804 2316 64-65 0.9680758334601407 2316 66-67 0.9031610475135281 2316 68-69 0.9062620237697416 2316 70-71 0.894302646682668 2316 72-73 0.9230496753252027 2316 74-75 0.970678967613793 2316 76-77 0.7829616652329179 2316 78-79 0.5356644165476752 2316 80-81 0.5929227390234857 2316 82-83 0.5305484601705643 2316 84-85 0.6315063562314656 2316 86-87 0.6982510681407277 2316 88-89 0.6716510268263924 2316 90-91 0.5086549431475262 2316 92-93 0.3633872145173278 2316 94-95 0.40846656985749163 2316 96-97 0.5981251008090531 2316 98-99 0.8368705398113505 2316 100 0.4991817354813044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 2.0 11 12.0 12 47.0 13 236.0 14 976.0 15 2834.0 16 6658.0 17 14197.0 18 24763.0 19 37358.0 20 55675.0 21 80611.0 22 99292.0 23 96843.0 24 93097.0 25 99917.0 26 117568.0 27 144047.0 28 177551.0 29 218721.0 30 271145.0 31 340086.0 32 432657.0 33 578830.0 34 855573.0 35 1179972.0 36 1860154.0 37 2501957.0 38 1671771.0 39 288759.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48009706942249 22.076224678083197 13.604959603015512 22.8387186494788 2 15.27275774995665 19.782361555589784 41.47341454394555 23.471466150508018 3 18.837663595234726 26.799155405840793 28.83458751199082 25.528593486933666 4 12.342579100865045 16.484695046504452 39.76501544813918 31.40771040449133 5 12.888344399737594 38.876457262218366 34.3526062406361 13.882592097407938 6 29.679359428106267 38.49514203709947 17.48790208723047 14.337596447563802 7 27.118052944724326 32.090915409423665 23.09004919117115 17.700982454680858 8 26.884674252916614 35.250616349849054 19.071680560887028 18.793028836347307 9 26.411345250754465 15.031943473212381 22.038289856852813 36.51842141918034 10-11 26.699289355747812 26.05305561296883 26.11232892430494 21.135326106978418 12-13 26.312832627638354 23.674844373815695 27.445397517802355 22.566925480743592 14-15 23.646418066456203 24.744579617412363 25.28888539102288 26.32011692510855 16-17 21.662705564789313 27.45804624954733 26.721552824816463 24.157695360846894 18-19 21.0262199434182 27.627378154085196 29.449559745029052 21.896842157467557 20-21 22.952253602244003 26.656356765250656 28.63731544387681 21.754074188628532 22-23 23.365940763755653 26.39282089631964 28.32645606680318 21.91478227312153 24-25 22.593127627009814 26.201490294448188 29.484454039018633 21.720928039523375 26-27 22.524671912184736 26.538054951376093 28.189439472357574 22.747833664081593 28-29 21.507325509703367 27.21052920200592 28.86676907261997 22.41537621567074 30-31 22.729866220166535 26.625854513892268 28.77718060444169 21.867098661499508 32-33 23.20701956652612 26.874998194395893 27.714647438297785 22.2033348007802 34-35 22.983577237709376 26.661643197237968 28.780648216025305 21.574131349027347 36-37 22.505735590256037 26.219956746863797 28.412992370490304 22.861315292389857 38-39 22.810379812518196 26.326718230971537 29.054123940571603 21.80877801593867 40-41 22.23678858460153 25.282080199794006 29.771543690695434 22.709587524909036 42-43 22.36661974175627 25.582258917606833 28.833662820921546 23.21745851971535 44-45 22.481465427145512 26.616349286370443 28.584084456256605 22.31810083022744 46-47 22.49919305652587 26.49580118264 27.792413335693194 23.212592425140937 48-49 23.579711413100068 26.374836774553636 27.797635154758144 22.247816657588153 50-51 22.941497515341062 26.10014991064472 27.825864541754758 23.132488032259456 52-53 22.203665227247708 26.866330214095957 27.42408450540845 23.505920053247877 54-55 22.219726453473694 26.750571903384063 27.324790811715253 23.70491083142699 56-57 23.487765463507035 26.077314513284904 26.953358307119984 23.481561716088077 58-59 22.148225752066384 26.865028148144066 27.846594425889283 23.14015167390027 60-61 22.356752663361426 27.572528046844585 27.02047495204794 23.050244337746047 62-63 22.908333168535403 26.596502002794825 26.512030656414975 23.983134172254797 64-65 21.83880795273137 26.952585108922616 26.999130006077067 24.20947693226895 66-67 22.50294748107649 26.424153399036864 27.08142589338447 23.99147322650217 68-69 22.032092286166407 26.410314524574442 27.357442456657704 24.20015073260144 70-71 21.918327097823866 25.966348346220524 27.576242708935812 24.539081847019798 72-73 22.687172737450457 26.126251335415045 27.773431801811405 23.413144125323097 74-75 22.942066990349268 26.609333745405088 27.167559982758426 23.281039281487214 76-77 22.619348897988736 26.413458698988222 26.6515788205119 24.31561358251114 78-79 22.456947883219996 26.487517781801834 26.816961689978534 24.238572644999632 80-81 21.85001943573141 27.015733321233864 27.498033651479098 23.636213591555627 82-83 23.069493310554826 25.693403567458102 28.793771274550018 22.443331847437058 84-85 23.347841786292005 26.294819512553865 27.085924512263297 23.271414188890834 86-87 21.6387414694459 27.23506775909102 27.604434409650775 23.521756361812304 88-89 22.770252773238127 25.746095235443907 27.458336110933352 24.025315880384618 90-91 22.456170912267396 25.749985583377892 27.632394054278258 24.16144945007645 92-93 22.932887930488345 26.09821194972146 27.325291156398773 23.643608963391415 94-95 21.53165177001756 26.08784813262506 28.153135665338446 24.227364432018927 96-97 21.906807788636485 26.031586990212674 27.642031709468984 24.419573511681858 98-99 21.193950466773117 26.69601848495155 27.468651696240727 24.6413793520346 100 22.016933373839287 25.95611967535846 26.5513776158725 25.475569334929745 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1349.0 1 887.5 2 603.0 3 813.0 4 677.5 5 608.5 6 1049.0 7 2099.0 8 3264.5 9 3782.5 10 3544.5 11 3392.5 12 3981.0 13 5111.5 14 6444.0 15 8145.5 16 10013.0 17 11711.0 18 13509.5 19 14936.0 20 15989.5 21 17075.5 22 18430.5 23 20024.5 24 21581.0 25 23534.0 26 27154.0 27 31418.0 28 38810.0 29 56967.0 30 84693.5 31 111585.5 32 151132.5 33 196023.5 34 226779.5 35 259656.0 36 287898.0 37 340852.0 38 364044.5 39 332994.0 40 299875.5 41 266548.5 42 258603.0 43 275718.5 44 310716.5 45 386027.0 46 459713.0 47 512834.0 48 555828.0 49 552268.5 50 523991.0 51 537041.5 52 543718.5 53 510057.5 54 482552.0 55 414125.5 56 353863.5 57 289848.0 58 224242.0 59 220290.0 60 180837.0 61 114952.0 62 80855.5 63 69316.5 64 49793.0 65 19876.5 66 9179.0 67 5966.5 68 4479.0 69 3522.5 70 2769.0 71 2206.5 72 1827.5 73 1734.5 74 1402.0 75 1030.5 76 717.0 77 400.5 78 235.5 79 161.0 80 116.5 81 72.5 82 42.5 83 24.0 84 16.5 85 11.0 86 7.5 87 6.5 88 3.5 89 1.0 90 2.5 91 2.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043879292040463515 2 0.0 3 0.0 4 0.032778373312787004 5 0.0 6 0.0 7 0.001022102204710007 8 0.0 9 0.011483096073785469 10-11 4.577240308049162E-4 12-13 8.887845258347886E-6 14-15 1.4664944676274014E-4 16-17 2.8885497089630634E-4 18-19 0.0 20-21 8.887845258347886E-6 22-23 0.0 24-25 4.843875665799598E-4 26-27 5.777099417926127E-4 28-29 1.955325956836535E-4 30-31 0.0023152836897996245 32-33 0.014429416776927793 34-35 0.02477042473501556 36-37 0.029778725538094592 38-39 0.021237506244822277 40-41 0.001177639496731095 42-43 1.0665414310017463E-4 44-45 0.011834165961490211 46-47 0.0185044938278803 48-49 0.0035062549544182414 50-51 0.011429769002235383 52-53 0.011514203532189687 54-55 0.01038989110700868 56-57 5.066071797258296E-4 58-59 0.008305691393926099 60-61 0.004452810474432291 62-63 0.0014976019260316189 64-65 0.0365823710833599 66-67 0.06198383283171816 68-69 0.06137501543152133 70-71 0.05376257596774636 72-73 0.04697670611299776 74-75 0.03634239926138451 76-77 0.03232064928198209 78-79 0.015624831964175585 80-81 0.0012131908777644866 82-83 0.039653121620119094 84-85 0.05366036574727536 86-87 0.05925970826003454 88-89 0.0720048783605054 90-91 0.04996302211980264 92-93 0.04064856028905406 94-95 0.013611735013159789 96-97 0.0034795914186431976 98-99 0.021215286631676405 100 0.03192514016798561 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1251321E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.71763174610999 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83883053570621 49.081095066654754 2 14.809523667525983 19.16874598100494 3 4.846947554959712 9.410489008637727 4 1.9594122921464963 5.072340926477529 5 0.9709708354074954 3.1419466481057534 6 0.4968660872693113 1.92935978778155 7 0.2915070195697566 1.320595076074511 8 0.19277460246834827 0.9980732586039447 9 0.13250783919863937 0.771803418665727 >10 0.43087343773299996 4.5047836392290685 >50 0.01832808835580522 0.8151841134193174 >100 0.009902117582124368 1.1834583883907979 >500 7.952256981691435E-4 0.3457952086047575 >1k 5.916528704783884E-4 0.7083169000756504 >5k 1.0142610513714034E-4 0.44790939144226 >10k+ 6.761740342476023E-5 1.1001031868315994 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35546 0.315927347553234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29792 0.26478668593670024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18384 0.16339414722946755 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 16835 0.14962687492428667 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.887845258347886E-6 0.0 10-11 0.0 0.0 0.0 8.887845258347886E-6 0.0 12-13 0.0 0.0 0.0 1.333176788752183E-5 0.0 14-15 0.0 0.0 0.0 2.2219613145869714E-5 0.0 16-17 0.0 0.0 0.0 4.4439226291739435E-5 0.0 18-19 0.0 0.0 0.0 7.11027620667831E-5 0.0 20-21 0.0 0.0 0.0 1.0665414310017464E-4 0.0 22-23 0.0 0.0 0.0 2.444157446045669E-4 0.0 24-25 0.0 0.0 0.0 5.688220965342648E-4 0.0 26-27 0.0 0.0 0.0 0.001390947782931444 0.0 28-29 0.0 0.0 0.0 0.004021749979402418 0.0 30-31 0.0 0.0 0.0 0.015567060969996323 0.0 32-33 0.0 0.0 0.0 0.04417703485661817 0.0 34-35 0.0 0.0 0.0 0.08665649126889188 0.0 36-37 0.0 0.0 0.0 0.15903910305287708 0.0 38-39 0.0 4.443922629173943E-6 0.0 0.26379124726776526 0.0 40-41 0.0 8.887845258347886E-6 0.0 0.3736894538872369 0.0 42-43 0.0 8.887845258347886E-6 0.0 0.4832054831605995 0.0 44-45 0.0 8.887845258347886E-6 0.0 0.6087240778216176 0.0 46-47 0.0 8.887845258347886E-6 0.0 0.7600129798092153 0.0 48-49 0.0 1.7775690516695772E-5 0.0 0.9297974877794349 0.0 50-51 0.0 2.666353577504366E-5 0.0 1.1051680064945262 0.0 52-53 0.0 2.666353577504366E-5 0.0 1.2716951191775614 0.0 54-55 0.0 2.666353577504366E-5 0.0 1.4493853655050817 0.0 56-57 0.0 2.666353577504366E-5 0.0 1.660476134313473 0.0 58-59 4.443922629173943E-6 2.666353577504366E-5 0.0 1.9072915971377937 0.0 60-61 8.887845258347886E-6 2.666353577504366E-5 0.0 2.1524628085893203 0.0 62-63 8.887845258347886E-6 2.666353577504366E-5 0.0 2.403486666143469 0.0 64-65 8.887845258347886E-6 2.666353577504366E-5 0.0 2.657585718157006 0.0 66-67 8.887845258347886E-6 2.666353577504366E-5 0.0 2.9407169167069362 0.0 68-69 8.887845258347886E-6 2.666353577504366E-5 0.0 3.2484007877830523 0.0 70-71 8.887845258347886E-6 2.666353577504366E-5 0.0 3.5855700855037376 0.0 72-73 8.887845258347886E-6 2.666353577504366E-5 0.0 3.927641029884402 0.0 74-75 8.887845258347886E-6 2.666353577504366E-5 0.0 4.248794430449545 0.0 76-77 8.887845258347886E-6 2.666353577504366E-5 0.0 4.616111299286546 0.0 78-79 8.887845258347886E-6 2.666353577504366E-5 0.0 5.009282909980081 0.0 80-81 8.887845258347886E-6 2.666353577504366E-5 0.0 5.425989534917722 4.443922629173943E-6 82-83 8.887845258347886E-6 2.666353577504366E-5 0.0 5.848135521153472 1.7775690516695772E-5 84-85 8.887845258347886E-6 2.666353577504366E-5 0.0 6.24764416551621 1.7775690516695772E-5 86-87 8.887845258347886E-6 2.666353577504366E-5 0.0 6.668528077725274 1.7775690516695772E-5 88 8.887845258347886E-6 2.666353577504366E-5 0.0 7.010981199452047 1.7775690516695772E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 13470 0.0 42.823063 94 GTACTAG 13920 0.0 37.491665 1 GGTATCA 31940 0.0 34.22465 1 TAGGCAT 16925 0.0 33.674355 5 ACCTGGG 4035 0.0 33.664787 3 TACCCCG 695 0.0 33.12672 5 TATTCTC 5595 0.0 32.751534 5 GTATCAA 49065 0.0 32.570946 1 CTGTGCG 19845 0.0 32.318027 9 TACCTGG 4470 0.0 31.963255 2 ACTGTGC 21030 0.0 31.144888 8 TAGTACT 20590 0.0 30.954582 4 CTAGGCA 19395 0.0 30.317202 4 TACTGTG 22940 0.0 29.801403 7 GTACTGT 22100 0.0 29.126982 6 AAGCTAA 7245 0.0 29.053797 9 CAACGCA 52660 0.0 28.35569 5 CTAACGC 7120 0.0 28.254354 3 GCATCGT 17670 0.0 27.97322 8 ATCAACG 53790 0.0 27.813566 3 >>END_MODULE