FastQCFastQC Report
Mon 6 Jun 2016
ERR1211208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098485
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171480.2811845892873394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136920.22451477703068876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.1348859593817153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95300.050.0196081
TACCTGG47100.042.4604532
GTATCAA162500.039.383041
GTACCTG59900.034.5660361
ACCTGGG58550.033.8007353
CAACGCA189000.032.1098985
ATCAACG189550.032.0164643
TATCAAC207300.030.3266032
AACGCAG209450.029.6483486
GTACTAG30550.029.574251
GTACATG217350.029.4227681
TCAACGC208400.029.414024
TACATGG223350.027.936642
GTGGTAT39200.027.3698561
ACATGGG220250.027.1055833
ACGCAGA229400.026.414647
CGCAGAG235650.025.752278
CATGGGG166850.025.7228494
CTGTGCG45000.025.4876889
GCATCGT32350.024.4113458
Produced by FastQC (version 0.11.5)