##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6098485 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53402722151485 31.0 31.0 34.0 30.0 34.0 2 31.549112607475465 33.0 31.0 34.0 28.0 34.0 3 31.61583770395434 33.0 31.0 34.0 28.0 34.0 4 35.209223602255314 37.0 35.0 37.0 32.0 37.0 5 35.26489874124475 37.0 35.0 37.0 32.0 37.0 6 35.324487803118316 37.0 35.0 37.0 32.0 37.0 7 35.248083909364375 37.0 35.0 37.0 32.0 37.0 8 35.244303134303024 37.0 35.0 37.0 32.0 37.0 9 36.82722954963405 39.0 37.0 39.0 32.0 39.0 10-11 36.77727492975714 39.0 37.0 39.0 32.0 39.0 12-13 36.67891336946799 39.0 37.0 39.0 32.0 39.0 14-15 37.91002519478198 40.0 38.0 41.0 32.0 41.0 16-17 37.881930102312296 40.0 38.0 41.0 32.0 41.0 18-19 37.89293316290849 40.0 38.0 41.0 32.0 41.0 20-21 37.85853232401162 40.0 38.0 41.0 32.0 41.0 22-23 37.75738941720772 40.0 37.0 41.0 32.0 41.0 24-25 37.67397345406277 40.0 37.0 41.0 32.0 41.0 26-27 37.54079578780632 40.0 37.0 41.0 32.0 41.0 28-29 37.421871005667796 40.0 37.0 41.0 31.0 41.0 30-31 37.27134681810318 40.0 36.5 41.0 31.0 41.0 32-33 37.088156812716605 40.0 36.0 41.0 30.5 41.0 34-35 36.95121083351029 39.0 36.0 41.0 30.0 41.0 36-37 36.85666218741212 39.0 36.0 41.0 30.0 41.0 38-39 36.73111920419579 39.0 35.0 41.0 30.0 41.0 40-41 36.576895737219985 39.0 35.0 41.0 30.0 41.0 42-43 36.38617451711367 39.0 35.0 40.5 29.0 41.0 44-45 36.19324856911184 39.0 35.0 40.0 28.5 41.0 46-47 35.982948633964014 39.0 35.0 40.0 28.0 41.0 48-49 35.8058147228369 38.5 35.0 40.0 27.0 41.0 50-51 35.131495527167814 37.5 33.5 39.5 26.5 40.5 52-53 35.23680020529689 38.0 34.0 40.0 26.5 40.5 54-55 35.73959139032071 38.5 35.0 40.0 28.0 41.0 56-57 35.7182433014101 38.0 35.0 40.0 27.5 41.0 58-59 35.5276793334738 38.0 34.0 40.0 27.0 41.0 60-61 35.30916325939967 38.0 34.0 40.0 27.0 41.0 62-63 35.01312662079188 37.0 34.0 40.0 26.0 41.0 64-65 34.76984439578027 37.0 34.0 40.0 26.0 41.0 66-67 34.599880298139624 36.5 34.0 39.5 26.0 41.0 68-69 34.302634670741995 36.0 33.0 39.0 26.0 41.0 70-71 33.985723257497554 35.5 33.0 39.0 26.0 41.0 72-73 33.63349126873314 35.0 33.0 38.5 25.0 40.0 74-75 33.292508221304146 35.0 33.0 37.0 25.0 40.0 76-77 32.94750466714274 35.0 32.0 37.0 24.5 39.0 78-79 32.603489965130684 35.0 32.0 36.5 24.0 39.0 80-81 32.29804271060763 35.0 32.0 36.0 23.5 38.5 82-83 32.00310339371172 35.0 31.5 35.5 23.0 37.0 84-85 31.743595827488303 35.0 31.0 35.0 23.0 37.0 86-87 31.5087383997829 35.0 31.0 35.0 21.5 36.5 88-89 31.28781410465058 34.0 31.0 35.0 20.0 36.0 90-91 31.05909205319026 34.0 31.0 35.0 20.0 36.0 92-93 30.86961647032009 34.0 30.5 35.0 20.0 35.5 94-95 30.694450261007447 34.0 30.0 35.0 19.0 35.0 96-97 30.50896427555368 34.0 30.0 35.0 18.5 35.0 98-99 30.277863190612095 34.0 29.5 35.0 18.0 35.0 100 29.154540348955518 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5070324636188275 1101 2 -0.3657657449148566 1101 3 -0.45979024457313145 1101 4 -0.3454392014150045 1101 5 -0.30262263591536964 1101 6 -0.36470785748777956 1101 7 -0.3554042846099108 1101 8 -0.30642811352034727 1101 9 -0.4757073158666145 1101 10-11 -0.45942974274102255 1101 12-13 -0.5072243123128928 1101 14-15 -0.6568369107482894 1101 16-17 -0.6692530582763396 1101 18-19 -0.6275999630306117 1101 20-21 -0.8009249600702546 1101 22-23 -0.7462917785224548 1101 24-25 -0.8140941542542919 1101 26-27 -0.7802638136825166 1101 28-29 -0.8103344102906078 1101 30-31 -0.859016930510542 1101 32-33 -0.8871063027360222 1101 34-35 -0.8589955964391578 1101 36-37 -0.8586545710033491 1101 38-39 -0.9092815296375392 1101 40-41 -1.0079833332862194 1101 42-43 -1.0084174784397746 1101 44-45 -1.0504324630780886 1101 46-47 -1.2283087185589636 1101 48-49 -1.278626624466959 1101 50-51 -1.289504271222448 1101 52-53 -1.1376552552498467 1101 54-55 -1.09720655193059 1101 56-57 -1.0334165542766343 1101 58-59 -0.9545767793746407 1101 60-61 -0.9197617642511418 1101 62-63 -1.0241042149785073 1101 64-65 -0.9273528360075858 1101 66-67 -0.9941604664511203 1101 68-69 -0.9327841836274544 1101 70-71 -1.1140374260033568 1101 72-73 -1.0483217074510307 1101 74-75 -1.0867474548593634 1101 76-77 -1.2157808913243926 1101 78-79 -1.099451417527618 1101 80-81 -1.2030500947086296 1101 82-83 -1.193843707945966 1101 84-85 -1.2089115322388722 1101 86-87 -1.2564197770494019 1101 88-89 -1.246903861138616 1101 90-91 -1.331618173312762 1101 92-93 -1.4647800898454655 1101 94-95 -1.4139942267787937 1101 96-97 -1.502682093209465 1101 98-99 -1.5967103546970023 1101 100 -1.6964597130113432 1102 1 -0.4006161215403843 1102 2 -0.3402030370625937 1102 3 -0.3584475488080585 1102 4 -0.20286016637592041 1102 5 -0.25412541655316545 1102 6 -0.31727385524897755 1102 7 -0.273370070884873 1102 8 -0.3798924393261487 1102 9 -0.4764477548404713 1102 10-11 -0.5169091517620217 1102 12-13 -0.4620393393130371 1102 14-15 -0.6296682480201525 1102 16-17 -0.6667897446894173 1102 18-19 -0.6581540180951251 1102 20-21 -0.7005102870542572 1102 22-23 -0.7576904727711806 1102 24-25 -0.7389766061288441 1102 26-27 -0.6411064198923881 1102 28-29 -0.756629773006587 1102 30-31 -0.8131703081487984 1102 32-33 -0.9296296602042062 1102 34-35 -0.8802778499485271 1102 36-37 -0.8594655949726899 1102 38-39 -0.9194522979010671 1102 40-41 -1.0161174912546471 1102 42-43 -1.0022712721738216 1102 44-45 -1.0171910562282278 1102 46-47 -1.0827440883919337 1102 48-49 -1.066762353801245 1102 50-51 -1.0769151961988968 1102 52-53 -0.9750407206215428 1102 54-55 -0.8689145997424745 1102 56-57 -0.8816808826750204 1102 58-59 -0.8613035215395897 1102 60-61 -0.7576946941196852 1102 62-63 -0.9077428817710071 1102 64-65 -0.8373305665600768 1102 66-67 -0.8358313184207091 1102 68-69 -0.9107240724019476 1102 70-71 -0.8422011553698994 1102 72-73 -0.8847324043106894 1102 74-75 -0.9048579522314384 1102 76-77 -0.9478391893138536 1102 78-79 -0.9523047665682896 1102 80-81 -1.0021797888281974 1102 82-83 -1.0718818940333108 1102 84-85 -1.0975623028167725 1102 86-87 -1.2359531003601383 1102 88-89 -1.1140363097328958 1102 90-91 -1.1618273657397893 1102 92-93 -1.1368836507596818 1102 94-95 -1.141384793556547 1102 96-97 -1.2706016000724922 1102 98-99 -1.3495296363994314 1102 100 -1.4383295466915946 1103 1 -0.489683110282936 1103 2 -0.4372358788080497 1103 3 -0.5535527465032395 1103 4 -0.433908673003522 1103 5 -0.3930302964560468 1103 6 -0.3990357804532465 1103 7 -0.4135274646775855 1103 8 -0.46187763861060205 1103 9 -0.6178603940841256 1103 10-11 -0.6141673317787308 1103 12-13 -0.625915374861826 1103 14-15 -0.7598447503244472 1103 16-17 -0.8209704144868226 1103 18-19 -0.6489623149636756 1103 20-21 -0.7336904977631562 1103 22-23 -0.7722842570417967 1103 24-25 -0.7479213978643742 1103 26-27 -0.8018439538079747 1103 28-29 -0.9519069504099917 1103 30-31 -0.8320836217466052 1103 32-33 -0.8804052528365673 1103 34-35 -0.9948536773088961 1103 36-37 -0.9684809106495749 1103 38-39 -0.9893632986736733 1103 40-41 -0.9651160947440331 1103 42-43 -1.0994340027540588 1103 44-45 -1.055365616020083 1103 46-47 -1.0774019774353008 1103 48-49 -1.2034342756190028 1103 50-51 -1.2714657007461696 1103 52-53 -1.1782239205274294 1103 54-55 -1.034417792099319 1103 56-57 -0.9322167652998417 1103 58-59 -0.9080065117609095 1103 60-61 -0.9643364849559859 1103 62-63 -0.895694788292019 1103 64-65 -0.8252290524371304 1103 66-67 -0.9410242488922407 1103 68-69 -0.8585808678275697 1103 70-71 -0.948465726929193 1103 72-73 -0.9123388645768742 1103 74-75 -0.9242749594896225 1103 76-77 -0.9616353887811329 1103 78-79 -1.0285005928049955 1103 80-81 -1.0737810206380942 1103 82-83 -0.9822790381861708 1103 84-85 -0.979155895567704 1103 86-87 -1.1632829745438116 1103 88-89 -1.1857336893897852 1103 90-91 -1.3127818756247827 1103 92-93 -1.326420747377174 1103 94-95 -1.3096273692200135 1103 96-97 -1.2612832828755458 1103 98-99 -1.306333225670727 1103 100 -1.3196706562827565 1104 1 -0.426878195448424 1104 2 -0.36922293729547917 1104 3 -0.487225076602968 1104 4 -0.24563622662314089 1104 5 -0.3103725568103499 1104 6 -0.32443867939365134 1104 7 -0.3798628962936661 1104 8 -0.31623085495319714 1104 9 -0.429941516829615 1104 10-11 -0.4527112810437046 1104 12-13 -0.4369350043861786 1104 14-15 -0.660413030029602 1104 16-17 -0.7717553935598147 1104 18-19 -0.7759150000518815 1104 20-21 -0.6450962242893752 1104 22-23 -0.6824988563939982 1104 24-25 -0.6736674988413967 1104 26-27 -0.7297367978807827 1104 28-29 -0.7320832803926933 1104 30-31 -0.69724953083999 1104 32-33 -0.7261340785554609 1104 34-35 -0.8807816623686833 1104 36-37 -0.818265142204055 1104 38-39 -0.820140937968354 1104 40-41 -0.8190483407681768 1104 42-43 -0.8345711632741981 1104 44-45 -0.8846279676966518 1104 46-47 -1.0205341102496206 1104 48-49 -0.8521377184719157 1104 50-51 -0.8654957746142529 1104 52-53 -0.7466922281239832 1104 54-55 -0.5856821719775169 1104 56-57 -0.7043281950066884 1104 58-59 -0.6576711077098025 1104 60-61 -0.74128729006474 1104 62-63 -0.7137575099678983 1104 64-65 -0.6939438005555445 1104 66-67 -0.7356963992151506 1104 68-69 -0.7899611342333515 1104 70-71 -0.7149139754472387 1104 72-73 -0.6996360852176693 1104 74-75 -0.6996955157290046 1104 76-77 -0.7152316202142188 1104 78-79 -0.7086474778362089 1104 80-81 -0.7883428639222814 1104 82-83 -0.8042068404466676 1104 84-85 -0.8495638039581799 1104 86-87 -0.8195507353625509 1104 88-89 -0.8188358718948052 1104 90-91 -0.9696260224266346 1104 92-93 -0.9441030180344896 1104 94-95 -0.9370424325342164 1104 96-97 -1.0394321064013319 1104 98-99 -1.1168946479845943 1104 100 -0.9589126340598284 1105 1 -0.5283145626260684 1105 2 -0.5235810220593251 1105 3 -0.5844991557161094 1105 4 -0.38161956051852286 1105 5 -0.35920466956387287 1105 6 -0.3077196894780627 1105 7 -0.4345611301062675 1105 8 -0.45932679252057085 1105 9 -0.6212807451837392 1105 10-11 -0.6482741589412626 1105 12-13 -0.6640244535598967 1105 14-15 -0.8381842878769561 1105 16-17 -0.8570041364062959 1105 18-19 -0.8522111576124161 1105 20-21 -0.8655530788145995 1105 22-23 -0.8134774760204522 1105 24-25 -0.8785315600074384 1105 26-27 -0.8749226129716163 1105 28-29 -0.8784237175104366 1105 30-31 -0.9141513700920427 1105 32-33 -1.034417841884327 1105 34-35 -0.9753123051772477 1105 36-37 -0.9213892679089284 1105 38-39 -0.9474623135528546 1105 40-41 -0.9645544343232757 1105 42-43 -1.0394581101225597 1105 44-45 -1.0611198523307834 1105 46-47 -1.0872607226715658 1105 48-49 -1.0582720712312153 1105 50-51 -1.0541405317314698 1105 52-53 -0.8783133572272632 1105 54-55 -0.8054081982717136 1105 56-57 -0.7038577822282903 1105 58-59 -0.7930612301887692 1105 60-61 -0.7290163413860071 1105 62-63 -0.7120439445039111 1105 64-65 -0.7060302712606301 1105 66-67 -0.6959189491560451 1105 68-69 -0.686687192071048 1105 70-71 -0.6918791426516719 1105 72-73 -0.6625097416601093 1105 74-75 -0.7860639466375829 1105 76-77 -0.7222673177011032 1105 78-79 -0.7172452442299431 1105 80-81 -0.6933286399863654 1105 82-83 -0.789914777243677 1105 84-85 -0.7964976734784273 1105 86-87 -0.8792659133589709 1105 88-89 -0.8526938312414778 1105 90-91 -0.918742886347097 1105 92-93 -0.9345311570828123 1105 94-95 -1.0158319639934597 1105 96-97 -1.0017046352819428 1105 98-99 -1.034305820811035 1105 100 -1.007322996381955 1106 1 -0.5834491684700147 1106 2 -0.5586576272127601 1106 3 -0.578059549642262 1106 4 -0.5055702909119901 1106 5 -0.48841478149188333 1106 6 -0.5100258126555062 1106 7 -0.5114216575236412 1106 8 -0.4539084986942825 1106 9 -0.6241460032954507 1106 10-11 -0.5791853487360541 1106 12-13 -0.6036964985091444 1106 14-15 -0.7552185253174457 1106 16-17 -0.803693593256881 1106 18-19 -0.8269179903021708 1106 20-21 -0.789821483787648 1106 22-23 -0.8432844136578339 1106 24-25 -0.7894798179081022 1106 26-27 -0.919394895894392 1106 28-29 -0.9297006057855057 1106 30-31 -0.9926856960960677 1106 32-33 -0.9766506370342896 1106 34-35 -0.9866289818329506 1106 36-37 -1.1020514665411554 1106 38-39 -1.1095274423061028 1106 40-41 -1.0687884535050358 1106 42-43 -1.0759014650693146 1106 44-45 -1.0410155034973911 1106 46-47 -1.0575443843266967 1106 48-49 -1.0856382923985564 1106 50-51 -1.09955670652311 1106 52-53 -1.0591906583865338 1106 54-55 -0.8420405428222111 1106 56-57 -0.8548668431371524 1106 58-59 -0.9727362195002272 1106 60-61 -0.9600438178753379 1106 62-63 -0.9666897182604473 1106 64-65 -0.9615991047121639 1106 66-67 -1.023010482574854 1106 68-69 -0.9864999810849326 1106 70-71 -0.9426122178191179 1106 72-73 -0.9002472751270432 1106 74-75 -0.87011350265972 1106 76-77 -0.9452453248644304 1106 78-79 -0.9036828071894867 1106 80-81 -0.8851487713542667 1106 82-83 -0.9201615844719555 1106 84-85 -0.8943215057179863 1106 86-87 -0.9489145901501068 1106 88-89 -1.0396171619231076 1106 90-91 -1.0593090892200827 1106 92-93 -0.9802306861746573 1106 94-95 -1.2024709760641237 1106 96-97 -1.1195855039012415 1106 98-99 -0.9600486263333075 1106 100 -1.2715422790584228 1107 1 -0.6558867133257209 1107 2 -0.605744394721917 1107 3 -0.5674347010659879 1107 4 -0.5115685446821558 1107 5 -0.4465537210458166 1107 6 -0.4906489698573324 1107 7 -0.524240630173658 1107 8 -0.5270878712400773 1107 9 -0.732583219018828 1107 10-11 -0.6153339976935754 1107 12-13 -0.741297489563145 1107 14-15 -0.8513914934967488 1107 16-17 -0.8153776536526607 1107 18-19 -0.8952611290360224 1107 20-21 -0.8372051560815663 1107 22-23 -0.9514100507803036 1107 24-25 -0.9815832934540225 1107 26-27 -0.9798680693242758 1107 28-29 -1.007409341963097 1107 30-31 -0.9665477439391026 1107 32-33 -1.1116681221890516 1107 34-35 -1.1175056623506023 1107 36-37 -1.0699798528327875 1107 38-39 -1.0119820380447564 1107 40-41 -1.0444983507883236 1107 42-43 -1.099070826506889 1107 44-45 -1.108922541900732 1107 46-47 -0.9423942862726946 1107 48-49 -1.0155677575128124 1107 50-51 -1.1083553735372007 1107 52-53 -0.978900381961104 1107 54-55 -0.9658520322831308 1107 56-57 -0.9500466545260551 1107 58-59 -0.9168559048131542 1107 60-61 -0.9533320326524759 1107 62-63 -0.9720728145727904 1107 64-65 -1.0042219236391077 1107 66-67 -0.9474576545510658 1107 68-69 -0.9366892778845752 1107 70-71 -0.9378640269040801 1107 72-73 -0.9001993680886429 1107 74-75 -0.8825198256573827 1107 76-77 -0.8289689085668925 1107 78-79 -0.9331454986690737 1107 80-81 -0.8377300830380108 1107 82-83 -0.8719536185554375 1107 84-85 -0.9165630339890818 1107 86-87 -0.8734770955785045 1107 88-89 -0.8563442609416541 1107 90-91 -1.0723511404458712 1107 92-93 -0.998456251527994 1107 94-95 -0.9999786215337529 1107 96-97 -1.034181791105766 1107 98-99 -1.0415531267607214 1107 100 -1.1672257086172486 1108 1 -0.5945456373939209 1108 2 -0.5174008737590228 1108 3 -0.6587794386385575 1108 4 -0.41086167083749103 1108 5 -0.43922807715809853 1108 6 -0.46843243695867187 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60-61 1.249612077814021 2311 62-63 1.2226184485389169 2311 64-65 1.2619979792801956 2311 66-67 1.2290288515311616 2311 68-69 1.1266253668508028 2311 70-71 1.1480256441140924 2311 72-73 1.1938537575324872 2311 74-75 1.1918537687912902 2311 76-77 1.22155703483228 2311 78-79 1.1750179559338818 2311 80-81 1.1731978339771203 2311 82-83 1.1760503641785505 2311 84-85 1.244144795095881 2311 86-87 1.2397899713616738 2311 88-89 1.3071241928300026 2311 90-91 1.3234260614647404 2311 92-93 1.3249450983013098 2311 94-95 1.363876842474756 2311 96-97 1.4201344124711959 2311 98-99 1.4458514082557095 2311 100 1.517640252257955 2312 1 0.5619311688062147 2312 2 0.5459920860479137 2312 3 0.6062398360192738 2312 4 0.2847093521782682 2312 5 0.37667561750644296 2312 6 0.4393566766700516 2312 7 0.3833161777292844 2312 8 0.5728794191028967 2312 9 0.6720915209028462 2312 10-11 0.6058445732781621 2312 12-13 0.7284330303742124 2312 14-15 0.9259818662235944 2312 16-17 0.9498418208365464 2312 18-19 0.9200975716413851 2312 20-21 0.9292817353863327 2312 22-23 0.979844812268567 2312 24-25 1.0118957166311446 2312 26-27 1.0647712199076693 2312 28-29 1.0735071497343185 2312 30-31 1.1017812311522235 2312 32-33 1.0618877464533512 2312 34-35 1.0415005122824184 2312 36-37 1.1468736974441498 2312 38-39 1.1848789678411649 2312 40-41 1.2296464744160147 2312 42-43 1.244732191918338 2312 44-45 1.3200687763349848 2312 46-47 1.3309985457440305 2312 48-49 1.2971816262116675 2312 50-51 1.3930069928146125 2312 52-53 1.2832353210863872 2312 54-55 1.1640057812133549 2312 56-57 1.0916097332679513 2312 58-59 1.1100703193777406 2312 60-61 1.1435059120115625 2312 62-63 1.1226292808553566 2312 64-65 1.1508219968417137 2312 66-67 1.0782542921486353 2312 68-69 1.0906024904287221 2312 70-71 1.0575869552079453 2312 72-73 1.1027162501330068 2312 74-75 1.1355982909242215 2312 76-77 1.0818213311638232 2312 78-79 1.1012925439411987 2312 80-81 1.0914117727142312 2312 82-83 1.0529134687683808 2312 84-85 1.1435907196724777 2312 86-87 1.1905434512027 2312 88-89 1.0264647859281766 2312 90-91 1.1193449557753432 2312 92-93 1.1756129211735136 2312 94-95 1.1616422790234076 2312 96-97 1.1708258563754974 2312 98-99 0.8504197772172475 2312 100 1.122532587118254 2313 1 0.6470067585455048 2313 2 0.5906626657853984 2313 3 0.6857735531785956 2313 4 0.5281992665922743 2313 5 0.34443046608030414 2313 6 0.5384306260372398 2313 7 0.5525524687765753 2313 8 0.4976719366794029 2313 9 0.7050670496086298 2313 10-11 0.6809094969137561 2313 12-13 0.7751413161631788 2313 14-15 0.9226415268950348 2313 16-17 0.9641710741613423 2313 18-19 0.9396964281913185 2313 20-21 0.98715970181469 2313 22-23 0.9854495985349914 2313 24-25 0.9632521836568628 2313 26-27 0.9945930482683494 2313 28-29 1.0731616940599267 2313 30-31 1.105645345989835 2313 32-33 1.1081159030413303 2313 34-35 1.1112047064132753 2313 36-37 1.196943165011092 2313 38-39 1.1837022213736716 2313 40-41 1.1791115584988603 2313 42-43 1.2808799830327686 2313 44-45 1.2711543707670003 2313 46-47 1.267299562542533 2313 48-49 1.2548729695063372 2313 50-51 1.3023504211843644 2313 52-53 1.2654421299342147 2313 54-55 1.0432778146839539 2313 56-57 1.0153127771808883 2313 58-59 1.036396480649877 2313 60-61 1.0312625757804952 2313 62-63 0.9889011931148204 2313 64-65 1.0464428119819829 2313 66-67 1.01396753998592 2313 68-69 0.9549411268182055 2313 70-71 1.0279920638745708 2313 72-73 1.0378443145030545 2313 74-75 0.9694802938138736 2313 76-77 0.7517357645877922 2313 78-79 0.9502924451574941 2313 80-81 1.0335413349272997 2313 82-83 1.0768283963882226 2313 84-85 1.0446754562356055 2313 86-87 1.0588981026767215 2313 88-89 1.063727836303073 2313 90-91 1.0945490681411876 2313 92-93 1.164942339930299 2313 94-95 1.2187053294537726 2313 96-97 1.2188957762156782 2313 98-99 1.2233660777557809 2313 100 1.3901932246507442 2314 1 0.5791391457709345 2314 2 0.5039073694431231 2314 3 0.5649368015710046 2314 4 0.39351158659266616 2314 5 0.42244217980849186 2314 6 0.4246501656187931 2314 7 0.5053840617519185 2314 8 0.45329752507452525 2314 9 0.6223287323532958 2314 10-11 0.6421745900805007 2314 12-13 0.6254503938219003 2314 14-15 0.8368451076602028 2314 16-17 0.8276281311230349 2314 18-19 0.7710796491631626 2314 20-21 0.7851577907061582 2314 22-23 0.8709204603997662 2314 24-25 0.8253132318495773 2314 26-27 0.8763701663829906 2314 28-29 0.9661308641434871 2314 30-31 0.960193499085328 2314 32-33 0.9543235262804757 2314 34-35 0.9298424074753484 2314 36-37 0.9417853337996576 2314 38-39 0.9717250709720915 2314 40-41 1.0471570144953546 2314 42-43 1.09772673188359 2314 44-45 1.1288110519390528 2314 46-47 1.137338822309978 2314 48-49 1.0139877214231916 2314 50-51 1.0451804907951256 2314 52-53 1.031076504475969 2314 54-55 0.9205283326813074 2314 56-57 0.8598730226502624 2314 58-59 0.8766605492342805 2314 60-61 0.8521117860656844 2314 62-63 0.8562110353039785 2314 64-65 0.8672038384061267 2314 66-67 0.8601695658195112 2314 68-69 0.8542379463169851 2314 70-71 0.8635202328872609 2314 72-73 0.8751041091617324 2314 74-75 0.9283146888939484 2314 76-77 0.9181947635525276 2314 78-79 0.9283843382589936 2314 80-81 0.9451679235768111 2314 82-83 0.9305472434144519 2314 84-85 0.9983302732936075 2314 86-87 0.9845691060075197 2314 88-89 1.0177218106465418 2314 90-91 1.1282002648842955 2314 92-93 1.0879851536744702 2314 94-95 1.1144406661598687 2314 96-97 1.2112188652683429 2314 98-99 1.2662232463253886 2314 100 1.3816670112489255 2315 1 0.41660447765384134 2315 2 0.4012162415410465 2315 3 0.42215152665977485 2315 4 0.1754887391193165 2315 5 0.21276622257645528 2315 6 0.2667677219779634 2315 7 0.3346952401575578 2315 8 0.344791218662273 2315 9 0.5307158849779512 2315 10-11 0.3689675728289288 2315 12-13 0.4212900972686313 2315 14-15 0.5993097045300289 2315 16-17 0.60873817238177 2315 18-19 0.4915439650946567 2315 20-21 0.6031391507743677 2315 22-23 0.5178650926808857 2315 24-25 0.6411887794343301 2315 26-27 0.7334154301220153 2315 28-29 0.6635002523185918 2315 30-31 0.6548314969750209 2315 32-33 0.7532900978854471 2315 34-35 0.7860718066919858 2315 36-37 0.8487030727687426 2315 38-39 0.9063711785061415 2315 40-41 0.9626525603160729 2315 42-43 1.050977245576064 2315 44-45 1.0661413864190123 2315 46-47 0.9995381611248675 2315 48-49 1.023917544007368 2315 50-51 0.959663351923389 2315 52-53 0.9238905673996172 2315 54-55 0.751463481660295 2315 56-57 0.7107745978649689 2315 58-59 0.7357928417890847 2315 60-61 0.7063188784519312 2315 62-63 0.7254591495870812 2315 64-65 0.7447786300707548 2315 66-67 0.7642744505531311 2315 68-69 0.6891113362299919 2315 70-71 0.6612387114428415 2315 72-73 0.4467444051923408 2315 74-75 0.6325554285457784 2315 76-77 0.6968911035140479 2315 78-79 0.7564729449028533 2315 80-81 0.4820960017299356 2315 82-83 0.4320877083347341 2315 84-85 0.8411697758367502 2315 86-87 0.9120300523123603 2315 88-89 0.8965191740729423 2315 90-91 0.9680643936324564 2315 92-93 1.059902753807755 2315 94-95 1.095678028018245 2315 96-97 0.9785935537044601 2315 98-99 0.4626356883001854 2315 100 0.9941182962151309 2316 1 0.2943034084433478 2316 2 0.2778555141411516 2316 3 0.2916022853394189 2316 4 0.15550013712336863 2316 5 0.0993261300971966 2316 6 0.1271389342463607 2316 7 0.06937269607124108 2316 8 0.16113521171985212 2316 9 0.24813119327453137 2316 10-11 0.17718036903131917 2316 12-13 0.010341456286326434 2316 14-15 0.03707158338737315 2316 16-17 0.2435628794983984 2316 18-19 0.2856828976974768 2316 20-21 0.2676638345768936 2316 22-23 0.2457766027221382 2316 24-25 0.36012678983500734 2316 26-27 0.4083496973145415 2316 28-29 0.3743009944840807 2316 30-31 0.3204328388309534 2316 32-33 0.5351284146074846 2316 34-35 0.5178876188321553 2316 36-37 0.45265197927272993 2316 38-39 0.5780645430544595 2316 40-41 0.6235457493610426 2316 42-43 0.557343694644473 2316 44-45 0.5306440513500732 2316 46-47 0.4194631350584146 2316 48-49 0.3350737452339487 2316 50-51 0.40026744613825116 2316 52-53 0.5636598063885643 2316 54-55 0.2685240594613916 2316 56-57 0.16650735676480366 2316 58-59 0.22967127171401813 2316 60-61 0.37356475477063356 2316 62-63 0.4718430356588428 2316 64-65 0.3165856508526872 2316 66-67 0.21971456893694352 2316 68-69 0.14232973429233198 2316 70-71 -0.0598951147246467 2316 72-73 -0.12746931062174127 2316 74-75 0.10998568053233271 2316 76-77 0.2757805914457663 2316 78-79 0.29560339376402567 2316 80-81 0.4788300040483975 2316 82-83 0.5276200631235071 2316 84-85 0.4831817825978426 2316 86-87 0.6125260435825339 2316 88-89 0.6554493645361816 2316 90-91 0.4427236677868329 2316 92-93 0.4362279336825061 2316 94-95 0.6612347654691071 2316 96-97 0.5439964828619814 2316 98-99 0.48938254575389806 2316 100 0.33739088336776746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 26.0 1 382.0 2 123.0 3 121.0 4 100.0 5 102.0 6 92.0 7 375.0 8 1271.0 9 2472.0 10 3956.0 11 6102.0 12 8668.0 13 11078.0 14 13285.0 15 15645.0 16 18936.0 17 23187.0 18 28179.0 19 33882.0 20 40379.0 21 49225.0 22 56024.0 23 57297.0 24 57584.0 25 59985.0 26 65307.0 27 75404.0 28 88555.0 29 106313.0 30 129436.0 31 161767.0 32 205622.0 33 271320.0 34 384697.0 35 539589.0 36 822141.0 37 1240707.0 38 1194605.0 39 324222.0 40 324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32355868880292 19.199008223078927 12.779858108040484 23.69757498007767 2 15.673571471262601 19.523297229736965 41.65151130035342 23.151619998647018 3 19.328052622981684 25.675712909017793 28.948019258006514 26.04821520999401 4 12.83141102732929 15.969689629322154 38.18776075445297 33.01113858889559 5 13.21820615401319 37.52633356278077 34.43848602740544 14.8169742558006 6 30.705909046836 36.563735645167455 18.334121652494147 14.396233655502394 7 27.909421780164536 31.97406172440672 22.342927262014946 17.7735892334138 8 25.981806348796464 36.07610169138384 19.872222157510645 18.069869802309043 9 26.66195644560179 16.239731113853544 21.052079876469065 36.0462325640756 10-11 25.613324097803115 26.170047764751526 27.611192379949383 20.605435757495975 12-13 25.558120729094735 23.68133999336119 28.123151186509187 22.63738809103489 14-15 23.01115702252307 25.1029439657636 26.005455958433227 25.8804430532801 16-17 22.064173784764147 27.873648354162473 26.937638864914753 23.124538996158623 18-19 21.785085712050577 27.381492324255426 28.899830854730972 21.93359110896303 20-21 22.869866532615678 26.808324992732295 28.513858655206732 21.807949819445295 22-23 22.91240101858079 26.55278337919943 28.344041892009475 22.190773710210305 24-25 22.376421906343722 26.441313542173322 28.842410022233196 22.339854529249763 26-27 22.344305432971666 26.662603118413834 28.350555578015413 22.642535870599087 28-29 21.9606232028632 27.225456538861614 28.423620174099582 22.390300084175603 30-31 22.957622518950608 26.784718414306337 28.453009928420613 21.804649138322446 32-33 22.817575549924793 26.79167060982332 28.131029031856496 22.25972480839539 34-35 22.773618892079412 26.85545187613195 28.442669634220795 21.92825959756784 36-37 22.61414546743096 26.772021347025625 28.1623564030211 22.451476782522317 38-39 22.635615750862282 26.939705011652908 28.286095726063976 22.138583511420833 40-41 22.598307583072945 26.310919489234063 28.68699668572589 22.403776241967105 42-43 22.37688660474952 26.625882837691055 28.160451495882743 22.836779061676683 44-45 22.301542638838683 27.07777892881512 28.287303185942864 22.33337524640333 46-47 22.35462822864048 26.758931622536466 27.887219896577196 22.999220252245856 48-49 22.649352805330444 26.718093268342102 27.931921610261938 22.700632316065512 50-51 22.565287296086762 26.804862056791244 27.895925774207054 22.733924872914944 52-53 22.31351455628079 27.17724137721741 27.61623088279115 22.893013183710654 54-55 22.250813637366864 27.025448737995767 27.78144807561978 22.942289549017588 56-57 22.628866097759662 26.86016252267421 27.423708205889174 23.087263173676956 58-59 22.15527207210045 27.07450455057056 27.932878366502734 22.837345010826255 60-61 22.549203719801216 27.242549736760097 27.657943940561907 22.550302602876776 62-63 22.698758057626105 27.027707252890647 27.371264756271646 22.902269933211596 64-65 22.19213455539923 26.901591570146486 27.632902290602683 23.273371583851592 66-67 22.35001126456859 26.73364663077041 27.500596854007338 23.415745250653668 68-69 22.08703497515835 26.800481454369486 27.78468245131631 23.327801119155854 70-71 22.310450487722015 26.63309324666219 27.721767397042612 23.334688868573174 72-73 22.490870703571005 26.914330616690947 27.5217938370277 23.073004842710343 74-75 22.65842497098313 27.056950152163783 27.332287498639673 22.952337378213414 76-77 22.48979234031289 26.804673476666036 27.238105906432224 23.46742827658885 78-79 22.37287621334344 26.79830679291917 27.42445227850665 23.404364715230745 80-81 22.19416852952462 27.08788045212442 27.54287183851635 23.175079179834604 82-83 22.771467987191972 26.792750953069906 27.912456893621496 22.523324166116627 84-85 22.985848462895518 27.01583658555885 27.185120407166746 22.813194544378888 86-87 22.215207648554827 27.450526824184347 27.436718163513728 22.8975473637471 88-89 22.592624391306334 26.601886399924297 27.56843244924071 23.23705675952866 90-91 22.792874376079844 26.665876144340523 27.49908175583537 23.042167723744264 92-93 22.897124834126938 26.79654200075673 27.4269293388547 22.879403826261633 94-95 22.36153335441452 26.856992152288377 27.58138999361004 23.200084499687062 96-97 22.521892541680153 26.718434232330623 27.406125294643026 23.353547931346196 98-99 22.28684241452698 26.93618305866426 27.241809523208115 23.535165003600646 100 22.77202924520952 26.74015967589157 26.999430021855996 23.488381057042922 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1121.0 1 820.5 2 1076.0 3 1535.5 4 1114.5 5 917.0 6 1245.0 7 2132.5 8 3456.5 9 4241.0 10 3930.0 11 3550.0 12 3834.5 13 4181.0 14 4685.0 15 5379.5 16 5878.0 17 6392.0 18 6926.5 19 7378.5 20 8000.5 21 8781.0 22 9992.0 23 11706.5 24 13744.0 25 16530.5 26 20525.0 27 25333.0 28 31054.5 29 39952.5 30 52294.0 31 65449.0 32 80701.0 33 97142.0 34 113183.5 35 129507.5 36 146485.0 37 168166.0 38 182917.5 39 187584.0 40 187553.0 41 184401.0 42 189004.5 43 199226.0 44 209259.5 45 227079.0 46 245321.5 47 259151.5 48 271125.0 49 272845.0 50 267150.0 51 264724.0 52 256947.5 53 241246.0 54 224970.0 55 199279.0 56 175002.0 57 150221.0 58 125022.5 59 109110.5 60 88906.5 61 67698.0 62 52709.5 63 41818.0 64 31297.0 65 20781.5 66 14385.5 67 10741.5 68 8363.0 69 6344.5 70 4761.5 71 3570.0 72 2720.5 73 2062.5 74 1541.5 75 1139.0 76 794.5 77 541.0 78 379.0 79 260.0 80 165.5 81 122.5 82 98.5 83 68.0 84 46.5 85 39.5 86 37.0 87 33.5 88 29.0 89 21.0 90 20.0 91 18.0 92 12.5 93 11.5 94 7.5 95 4.5 96 5.0 97 3.5 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05847353891991208 2 0.1350007419875592 3 0.017594533724359412 4 0.01695503063465762 5 0.020136148568046 6 0.053095153960368846 7 0.05827676873846537 8 0.05020919129915053 9 0.04135453313404887 10-11 0.04037888098437563 12-13 0.04123155177064468 14-15 0.018275030601862592 16-17 0.01734037223999075 18-19 0.017438757330714105 20-21 0.021357763444527614 22-23 0.02575229749683733 24-25 0.024932421740809396 26-27 0.019455651690542815 28-29 0.01824223557162148 30-31 0.016225341211792765 32-33 0.02335006153167549 34-35 0.02348944041020024 36-37 0.023440247864838563 38-39 0.021234782081123428 40-41 0.024989813043731355 42-43 0.03199155200020989 44-45 0.04155950207305585 46-47 0.04794633421251343 48-49 0.04071503004434708 50-51 0.027588819190339894 52-53 0.04000173813660278 54-55 0.03688621026369664 56-57 0.03855055804843334 58-59 0.04355180016020372 60-61 0.022710558441973703 62-63 0.02530956458858225 64-65 0.02438310498427068 66-67 0.03173739051584123 68-69 0.03890310462352535 70-71 0.04086260768043211 72-73 0.04468322870352227 74-75 0.02624422295045409 76-77 0.020095154780244602 78-79 0.019349067842259184 80-81 0.02371900562188806 82-83 0.019914782113918457 84-85 0.01209316740141199 86-87 0.01414285679148182 88-89 0.01809465793553645 90-91 0.020505092658258568 92-93 0.019709813174911474 94-95 0.023300868986313814 96-97 0.024407701256951523 98-99 0.027277266403049283 100 0.02885962661218319 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6098485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.37834521120091 #Duplication Level Percentage of deduplicated Percentage of total 1 79.33734419989638 53.45618965640486 2 13.083869141791041 17.63138903467551 3 3.7945792863641503 7.670174192637484 4 1.4791721514629435 3.9865668739225986 5 0.7479309941396539 2.5197176358649145 6 0.41503429769972233 1.677859451094013 7 0.2680611360103067 1.2643061031866385 8 0.17650523223490158 0.9514104375285107 9 0.12327326556664782 0.7475353778395478 >10 0.5357321130716173 6.014096855849902 >50 0.02481354392050943 1.140804866778883 >100 0.012620212033879495 1.535827249407774 >500 6.616720492072395E-4 0.2969762536996236 >1k 3.1644938219131636E-4 0.34991070755172793 >5k 2.8768125649105706E-5 0.1594485641495779 >10k+ 5.753625129821141E-5 0.5977867394083373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17148 0.2811845892873394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13692 0.22451477703068876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8226 0.1348859593817153 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.639751512055863E-5 2 0.0 0.0 0.0 0.0 1.639751512055863E-5 3 0.0 0.0 0.0 0.0 1.639751512055863E-5 4 0.0 0.0 0.0 0.0 1.639751512055863E-5 5 0.0 1.639751512055863E-5 0.0 0.0 1.639751512055863E-5 6 3.279503024111726E-5 1.639751512055863E-5 0.0 0.0 1.639751512055863E-5 7 3.279503024111726E-5 1.639751512055863E-5 0.0 1.639751512055863E-5 1.639751512055863E-5 8 3.279503024111726E-5 1.639751512055863E-5 0.0 1.639751512055863E-5 1.639751512055863E-5 9 3.279503024111726E-5 1.639751512055863E-5 0.0 1.639751512055863E-5 1.639751512055863E-5 10-11 4.919254536167589E-5 1.639751512055863E-5 0.0 3.279503024111726E-5 1.639751512055863E-5 12-13 6.559006048223452E-5 2.4596272680837945E-5 0.0 4.919254536167589E-5 1.639751512055863E-5 14-15 6.559006048223452E-5 3.279503024111726E-5 0.0 4.919254536167589E-5 1.639751512055863E-5 16-17 9.838509072335178E-5 3.279503024111726E-5 0.0 4.919254536167589E-5 2.4596272680837945E-5 18-19 9.838509072335178E-5 3.279503024111726E-5 0.0 8.198757560279316E-5 3.279503024111726E-5 20-21 9.838509072335178E-5 3.279503024111726E-5 0.0 1.065838482836311E-4 4.099378780139658E-5 22-23 9.838509072335178E-5 3.279503024111726E-5 0.0 1.721739087658656E-4 5.7391302921955206E-5 24-25 9.838509072335178E-5 3.279503024111726E-5 0.0 4.3453415069480373E-4 6.559006048223452E-5 26-27 1.1478260584391041E-4 3.279503024111726E-5 0.0 0.0012380123916021766 6.559006048223452E-5 28-29 1.1478260584391041E-4 3.279503024111726E-5 0.0 0.004263353931345244 6.559006048223452E-5 30-31 1.1478260584391041E-4 3.279503024111726E-5 0.0 0.0158154033337788 7.378881804251383E-5 32-33 1.1478260584391041E-4 4.919254536167589E-5 0.0 0.04701987460820187 1.1478260584391041E-4 34-35 1.3118012096446905E-4 4.919254536167589E-5 0.0 0.09388397282275844 1.3118012096446905E-4 36-37 1.3118012096446905E-4 5.7391302921955206E-5 0.0 0.1700094367699519 1.3118012096446905E-4 38-39 1.4757763608502767E-4 8.198757560279316E-5 0.0 0.2836852103432246 1.3118012096446905E-4 40-41 1.8037266632614494E-4 9.838509072335178E-5 0.0 0.41030682210417835 1.3118012096446905E-4 42-43 1.8037266632614494E-4 9.838509072335178E-5 0.0 0.5299594899388946 1.3118012096446905E-4 44-45 1.8037266632614494E-4 9.838509072335178E-5 0.0 0.6689284305856291 1.3118012096446905E-4 46-47 1.8037266632614494E-4 9.838509072335178E-5 0.0 0.8284680539511042 1.3118012096446905E-4 48-49 1.8037266632614494E-4 9.838509072335178E-5 0.0 1.0025604719860752 1.3118012096446905E-4 50-51 2.0496893900698288E-4 9.838509072335178E-5 0.0 1.1841875482189428 1.3118012096446905E-4 52-53 2.2956521168782083E-4 9.838509072335178E-5 0.0 1.3597885376450054 1.6397515120558632E-4 54-55 2.3776396924810012E-4 1.1478260584391041E-4 0.0 1.5555994644571562 1.9677018144670356E-4 56-57 2.4596272680837945E-4 1.1478260584391041E-4 0.0 1.7835085271178006 1.9677018144670356E-4 58-59 2.4596272680837945E-4 1.1478260584391041E-4 0.0 2.041589017600273 1.9677018144670356E-4 60-61 2.4596272680837945E-4 1.1478260584391041E-4 0.0 2.2953323653333575 2.0496893900698288E-4 62-63 2.7875775704949674E-4 1.1478260584391041E-4 0.0 2.5517895018188943 2.131676965672622E-4 64-65 2.7875775704949674E-4 1.1478260584391041E-4 0.0 2.8130511102347553 2.131676965672622E-4 66-67 2.9515527217005534E-4 1.1478260584391041E-4 0.0 3.1104774382490077 2.131676965672622E-4 68-69 3.0335402973033466E-4 1.2298136340418972E-4 0.0 3.4303683619784255 2.131676965672622E-4 70-71 3.11552787290614E-4 1.4757763608502767E-4 0.0 3.764115185984716 2.2956521168782083E-4 72-73 3.3614905997145195E-4 1.6397515120558632E-4 0.0 4.090146979126783 2.2956521168782083E-4 74-75 3.443478175317312E-4 1.8037266632614494E-4 0.0 4.41733479708485 2.2956521168782083E-4 76-77 3.443478175317312E-4 1.8037266632614494E-4 0.0 4.783860253817137 2.2956521168782083E-4 78-79 3.5254657509201055E-4 1.9677018144670356E-4 0.0 5.1814672004604425 2.2956521168782083E-4 80-81 3.689440902125692E-4 1.9677018144670356E-4 0.0 5.591232904565642 2.3776396924810012E-4 82-83 3.771428477728485E-4 1.9677018144670356E-4 0.0 6.007418235840541 2.4596272680837945E-4 84-85 3.771428477728485E-4 1.9677018144670356E-4 0.0 6.425522076384545 2.4596272680837945E-4 86-87 3.853416053331278E-4 1.9677018144670356E-4 0.0 6.863065171103971 2.4596272680837945E-4 88 3.935403628934071E-4 1.9677018144670356E-4 0.0 7.223679323635296 2.4596272680837945E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9530 0.0 50.019608 1 TACCTGG 4710 0.0 42.460453 2 GTATCAA 16250 0.0 39.38304 1 GTACCTG 5990 0.0 34.566036 1 ACCTGGG 5855 0.0 33.800735 3 CAACGCA 18900 0.0 32.109898 5 ATCAACG 18955 0.0 32.016464 3 TATCAAC 20730 0.0 30.326603 2 AACGCAG 20945 0.0 29.648348 6 GTACTAG 3055 0.0 29.57425 1 GTACATG 21735 0.0 29.422768 1 TCAACGC 20840 0.0 29.41402 4 TACATGG 22335 0.0 27.93664 2 GTGGTAT 3920 0.0 27.369856 1 ACATGGG 22025 0.0 27.105583 3 ACGCAGA 22940 0.0 26.41464 7 CGCAGAG 23565 0.0 25.75227 8 CATGGGG 16685 0.0 25.722849 4 CTGTGCG 4500 0.0 25.487688 9 GCATCGT 3235 0.0 24.411345 8 >>END_MODULE