FastQCFastQC Report
Mon 6 Jun 2016
ERR1211208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098485
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155770.2554240930329418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132840.21782459086150086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80900.13265589732531932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95750.048.6651761
TACCTGG46700.040.5555842
GTATCAA163700.038.6328741
CAACGCA189550.032.397735
ATCAACG190500.032.221813
ACCTGGG57950.032.0365223
GTACCTG60900.031.501141
GTACTAG34450.030.8462681
TATCAAC208700.030.1072772
AACGCAG212350.029.6051086
TCAACGC212150.029.1994084
GTACATG220800.028.8977581
TACATGG221700.028.2146632
ACATGGG219100.027.3722173
ACGCAGA234150.026.2668657
CGCAGAG242400.025.3147348
TGGGGGG121000.025.162426
CATGGGG167250.025.0668874
CCTGGGG55750.024.8701764
CTGTGCG43700.024.8366669
Produced by FastQC (version 0.11.5)