##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6098485 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2092559053601 33.0 31.0 34.0 30.0 34.0 2 32.350517874521294 34.0 31.0 34.0 31.0 34.0 3 32.43060219054404 34.0 31.0 34.0 31.0 34.0 4 36.02921512474 37.0 35.0 37.0 35.0 37.0 5 35.91555099340246 37.0 35.0 37.0 35.0 37.0 6 35.94154236666976 37.0 35.0 37.0 35.0 37.0 7 35.885612574270496 37.0 35.0 37.0 35.0 37.0 8 35.870417324958574 37.0 35.0 37.0 35.0 37.0 9 37.494022203875225 39.0 37.0 39.0 35.0 39.0 10-11 37.472905811853266 39.0 37.0 39.0 35.0 39.0 12-13 37.37570232606951 39.0 37.0 39.0 34.0 39.0 14-15 38.62704188007349 40.0 38.0 41.0 34.0 41.0 16-17 38.5726543559589 40.0 38.0 41.0 34.0 41.0 18-19 38.59315616911414 40.0 38.0 41.0 34.0 41.0 20-21 38.55212081361191 40.0 38.0 41.0 34.0 41.0 22-23 38.429325316041606 40.0 38.0 41.0 34.0 41.0 24-25 38.37042199825038 40.0 38.0 41.0 34.0 41.0 26-27 38.24174003871453 40.0 38.0 41.0 34.0 41.0 28-29 38.2211293460589 40.0 38.0 41.0 34.0 41.0 30-31 38.24380792934639 40.0 38.0 41.0 34.0 41.0 32-33 38.12327446898697 40.0 37.5 41.0 33.5 41.0 34-35 37.994062459774845 40.0 37.0 41.0 33.0 41.0 36-37 37.85539408558027 40.0 37.0 41.0 33.0 41.0 38-39 37.77928895455183 40.0 37.0 41.0 33.0 41.0 40-41 37.68815787855508 40.0 37.0 41.0 33.0 41.0 42-43 37.56246953136721 40.0 37.0 41.0 32.5 41.0 44-45 37.44489746223857 40.0 36.5 41.0 32.0 41.0 46-47 37.23408338300414 40.0 36.0 41.0 31.5 41.0 48-49 37.02264898577269 39.0 36.0 41.0 31.0 41.0 50-51 36.84087154432618 39.0 35.5 41.0 31.0 41.0 52-53 36.652111712991015 39.0 35.0 41.0 30.5 41.0 54-55 36.430148717263386 39.0 35.0 40.0 30.0 41.0 56-57 36.18951977417342 38.5 35.0 40.0 29.5 41.0 58-59 35.935267693533724 38.0 35.0 40.0 29.0 41.0 60-61 35.67434231616541 38.0 34.0 40.0 29.0 41.0 62-63 35.37285842303457 37.0 34.0 40.0 28.0 41.0 64-65 35.00506371664438 37.0 34.0 39.5 28.0 41.0 66-67 34.69804648203611 36.0 34.0 39.0 28.0 41.0 68-69 34.36608567537675 36.0 33.5 39.0 27.0 40.0 70-71 34.02819642911313 35.0 33.0 38.5 26.0 40.0 72-73 33.675205563348925 35.0 33.0 37.5 26.0 40.0 74-75 33.3286475247541 35.0 32.5 37.0 26.0 39.0 76-77 32.30985867801593 34.0 31.0 36.0 25.5 38.5 78-79 32.79467400510127 35.0 32.0 36.0 26.0 38.5 80-81 32.710841709047415 35.0 32.0 36.0 26.0 37.5 82-83 32.45518772285248 35.0 32.0 35.0 26.0 37.0 84-85 32.194864298264235 34.5 32.0 35.0 25.0 36.5 86-87 31.953350545258374 34.0 32.0 35.0 25.0 36.0 88-89 31.73814512948708 34.0 31.0 35.0 25.0 36.0 90-91 31.524634560878646 34.0 31.0 35.0 24.0 35.5 92-93 31.35364291295297 34.0 31.0 35.0 24.0 35.0 94-95 31.169974346087592 34.0 31.0 35.0 23.0 35.0 96-97 31.00518202471597 34.0 31.0 35.0 23.0 35.0 98-99 30.835023288570852 34.0 30.0 35.0 22.0 35.0 100 29.029775099881363 32.0 27.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.317629024433284 1101 2 -0.3109747093590798 1101 3 -0.32663831487814576 1101 4 -0.21617880957499835 1101 5 -0.27136887652438446 1101 6 -0.30902557260553465 1101 7 -0.3068383113082973 1101 8 -0.2746797561350789 1101 9 -0.4148264686587879 1101 10-11 -0.4704857636858506 1101 12-13 -0.40046249104668874 1101 14-15 -0.6452257484647745 1101 16-17 -0.5612108496285941 1101 18-19 -0.5842918838888025 1101 20-21 -0.5993994807380929 1101 22-23 -0.7365884212913869 1101 24-25 -0.6992603480467423 1101 26-27 -0.8061386414549929 1101 28-29 -0.7389416964588094 1101 30-31 -0.5871755186444076 1101 32-33 -0.6103677601286464 1101 34-35 -0.6543720299859075 1101 36-37 -0.6128050912424001 1101 38-39 -0.6940757457452378 1101 40-41 -0.7530799455285191 1101 42-43 -0.7439833419402788 1101 44-45 -0.7262292034042162 1101 46-47 -0.7226005216935292 1101 48-49 -0.6603636977772851 1101 50-51 -0.7377830180331131 1101 52-53 -0.7400779993387232 1101 54-55 -0.6104380456313052 1101 56-57 -0.6806113721062559 1101 58-59 -0.7132429310505941 1101 60-61 -0.7598180737775095 1101 62-63 -0.721580014910451 1101 64-65 -0.7626873838714303 1101 66-67 -0.8674906066310442 1101 68-69 -0.9053156485135929 1101 70-71 -0.9296788716319568 1101 72-73 -0.966786287710363 1101 74-75 -0.9753582950750186 1101 76-77 -0.9912485306220802 1101 78-79 -0.7626210546777159 1101 80-81 -0.6400162631494126 1101 82-83 -0.6836192993325838 1101 84-85 -0.7606345256732574 1101 86-87 -0.7936617021817405 1101 88-89 -0.8657857456030342 1101 90-91 -0.8988759264200787 1101 92-93 -0.8763557538494737 1101 94-95 -0.9592932497845794 1101 96-97 -0.946824244754449 1101 98-99 -1.0528151226530795 1101 100 -0.9151452903199164 1102 1 -0.3696897664183325 1102 2 -0.3036265662977584 1102 3 -0.3268754741395554 1102 4 -0.23231670530744708 1102 5 -0.3260674580426226 1102 6 -0.3712446731740542 1102 7 -0.3537279949436609 1102 8 -0.4381194159233033 1102 9 -0.5297430463630661 1102 10-11 -0.5279406378999099 1102 12-13 -0.5106962562210811 1102 14-15 -0.6557817068738458 1102 16-17 -0.6500238471322746 1102 18-19 -0.6724949100485631 1102 20-21 -0.6884418280994211 1102 22-23 -0.6278654279564151 1102 24-25 -0.734526334915877 1102 26-27 -0.788367758295216 1102 28-29 -0.707766287923441 1102 30-31 -0.7183234203730251 1102 32-33 -0.6611193045805166 1102 34-35 -0.7303065144632939 1102 36-37 -0.8284274082419074 1102 38-39 -0.8605705264867467 1102 40-41 -0.7678175076697968 1102 42-43 -0.7133942077929021 1102 44-45 -0.8610945077883727 1102 46-47 -0.8764551659253854 1102 48-49 -0.8269752559793915 1102 50-51 -0.913986057472016 1102 52-53 -0.9617508653124389 1102 54-55 -0.9778856210564797 1102 56-57 -0.9281480822320987 1102 58-59 -1.0302639849818007 1102 60-61 -0.9809199047665231 1102 62-63 -0.963955025324303 1102 64-65 -0.9289826928384812 1102 66-67 -1.0325619891658349 1102 68-69 -1.10623083475285 1102 70-71 -1.0685583043973423 1102 72-73 -1.0938342524675022 1102 74-75 -1.1138238768669098 1102 76-77 -0.9696853757051684 1102 78-79 -0.769866205298829 1102 80-81 -0.7147088311105918 1102 82-83 -0.7750486209878602 1102 84-85 -0.8458557981729129 1102 86-87 -0.9731137381359822 1102 88-89 -0.8579195892982909 1102 90-91 -0.8790435029455175 1102 92-93 -0.951363132471343 1102 94-95 -0.9355055055846044 1102 96-97 -1.0819381063354534 1102 98-99 -1.154147084440119 1102 100 -1.0881174181785092 1103 1 -0.3348243377075768 1103 2 -0.28947493796140833 1103 3 -0.353322852322016 1103 4 -0.20640946229379864 1103 5 -0.21008068624809795 1103 6 -0.286127397633102 1103 7 -0.3874741975305085 1103 8 -0.3971069814225743 1103 9 -0.5050828541170347 1103 10-11 -0.5397183689100515 1103 12-13 -0.5581199595288453 1103 14-15 -0.6947644362683363 1103 16-17 -0.700622835026806 1103 18-19 -0.6351040289307619 1103 20-21 -0.7258827154097958 1103 22-23 -0.752865746121202 1103 24-25 -0.8100106515646459 1103 26-27 -0.7657239547950283 1103 28-29 -0.8639529944708997 1103 30-31 -0.725237963794136 1103 32-33 -0.6826412279968608 1103 34-35 -0.7778221285942806 1103 36-37 -0.8410730462336957 1103 38-39 -0.702383463594721 1103 40-41 -0.6592271670286038 1103 42-43 -0.7289633145817334 1103 44-45 -0.7214981704393324 1103 46-47 -0.7728072591858464 1103 48-49 -0.8710312393243171 1103 50-51 -0.9103379024087417 1103 52-53 -0.8913869220432389 1103 54-55 -0.9183551733070487 1103 56-57 -0.9748492876265544 1103 58-59 -0.9138979607596838 1103 60-61 -0.9143143722998985 1103 62-63 -0.9340647161271036 1103 64-65 -0.9717598089369659 1103 66-67 -1.0761768409119057 1103 68-69 -1.1163454330535103 1103 70-71 -1.2049055584489707 1103 72-73 -1.0359898452923133 1103 74-75 -0.9270040782636642 1103 76-77 -0.9247677555529528 1103 78-79 -0.7531234894876526 1103 80-81 -0.8126590738625623 1103 82-83 -0.8654774015848332 1103 84-85 -0.9481745986699366 1103 86-87 -1.0018051403301094 1103 88-89 -1.017278631588134 1103 90-91 -1.0020294189543577 1103 92-93 -1.113128400733924 1103 94-95 -1.0609335613866762 1103 96-97 -1.0669793637723615 1103 98-99 -1.088488146539369 1103 100 -1.1895826523896957 1104 1 -0.31427428836265037 1104 2 -0.3216222843432135 1104 3 -0.337583253293225 1104 4 -0.20717970982362033 1104 5 -0.23545907896117768 1104 6 -0.33303095057664933 1104 7 -0.26798566558651515 1104 8 -0.2985573100079222 1104 9 -0.44515606087959014 1104 10-11 -0.5445814405159908 1104 12-13 -0.5244048251381273 1104 14-15 -0.6355124408001132 1104 16-17 -0.6356689102402555 1104 18-19 -0.7310367810381209 1104 20-21 -0.8036409626257708 1104 22-23 -0.7019057597005798 1104 24-25 -0.6956787642236506 1104 26-27 -0.7334548328953474 1104 28-29 -0.8374214670945861 1104 30-31 -0.7912969961161238 1104 32-33 -0.6710642419920063 1104 34-35 -0.7258284254978022 1104 36-37 -0.6716951187399687 1104 38-39 -0.7533761834196397 1104 40-41 -0.7968916244272393 1104 42-43 -0.6433018997273336 1104 44-45 -0.7494653432174232 1104 46-47 -0.8390084687319828 1104 48-49 -0.8513051468900201 1104 50-51 -0.8251193103670715 1104 52-53 -0.8153743052653795 1104 54-55 -0.838415726780255 1104 56-57 -0.830618231260658 1104 58-59 -0.9052126068151622 1104 60-61 -0.7829585456269967 1104 62-63 -0.7609246233226159 1104 64-65 -0.8173962980445069 1104 66-67 -0.9080633053862783 1104 68-69 -0.9356989933537605 1104 70-71 -0.9530089553844476 1104 72-73 -0.8946476403097492 1104 74-75 -0.8800416475070563 1104 76-77 -0.8299851156338178 1104 78-79 -0.6765149615381603 1104 80-81 -0.6506243702881704 1104 82-83 -0.6870418419004451 1104 84-85 -0.7595854875834576 1104 86-87 -0.7442294880830254 1104 88-89 -0.8099689148266833 1104 90-91 -0.7896605324087638 1104 92-93 -0.8534028620170453 1104 94-95 -0.7915546606559971 1104 96-97 -0.8610525199991379 1104 98-99 -0.9006250943981655 1104 100 -1.081166474024016 1105 1 -0.3768211306016518 1105 2 -0.32492266612846166 1105 3 -0.4117104324203673 1105 4 -0.22255477134402923 1105 5 -0.2806787351952238 1105 6 -0.3288097530706082 1105 7 -0.42501362553318245 1105 8 -0.4362529530765187 1105 9 -0.6189279549246933 1105 10-11 -0.5581706922855574 1105 12-13 -0.5336947774699112 1105 14-15 -0.8016792954664069 1105 16-17 -0.7269241154464154 1105 18-19 -0.7072769625203748 1105 20-21 -0.7833882235637759 1105 22-23 -0.7965277636060364 1105 24-25 -0.7780114745780367 1105 26-27 -0.8323113994242419 1105 28-29 -0.7953171243668464 1105 30-31 -0.7794093674802767 1105 32-33 -0.7664138804254677 1105 34-35 -0.7884645249250752 1105 36-37 -0.7564610826688707 1105 38-39 -0.8221439656099321 1105 40-41 -0.9080339124033472 1105 42-43 -0.829431593010149 1105 44-45 -0.8489376435802569 1105 46-47 -0.8674400844666081 1105 48-49 -0.9407590884553656 1105 50-51 -0.9752840630621478 1105 52-53 -0.9389253857753346 1105 54-55 -0.912789154032879 1105 56-57 -0.9362662753794169 1105 58-59 -1.0086005989882594 1105 60-61 -0.9464585825891874 1105 62-63 -0.9877658415504911 1105 64-65 -0.9779166100865453 1105 66-67 -0.9474831061149516 1105 68-69 -0.846125250084981 1105 70-71 -0.9416991902272684 1105 72-73 -0.9758073383567805 1105 74-75 -0.9258065490680494 1105 76-77 -0.9576924752030678 1105 78-79 -0.915887543897405 1105 80-81 -0.8180446095985445 1105 82-83 -0.8612648351454766 1105 84-85 -0.8647514339061217 1105 86-87 -0.8762359503216182 1105 88-89 -0.813369043071269 1105 90-91 -0.8151353997217541 1105 92-93 -0.9954710326524534 1105 94-95 -0.9740900455232442 1105 96-97 -1.079122043674829 1105 98-99 -1.0263084938689033 1105 100 -1.1016161328790162 1106 1 -0.4851510368051315 1106 2 -0.37029474283567865 1106 3 -0.4443497803773049 1106 4 -0.34797293782006733 1106 5 -0.35725073470860025 1106 6 -0.4055482875227412 1106 7 -0.3421825756571266 1106 8 -0.4172840419046011 1106 9 -0.5445379916915698 1106 10-11 -0.5765258885338156 1106 12-13 -0.663463378397033 1106 14-15 -0.7059824725673067 1106 16-17 -0.6603917083902076 1106 18-19 -0.777270066626933 1106 20-21 -0.7418500321736161 1106 22-23 -0.7218317465033905 1106 24-25 -0.7203081132233606 1106 26-27 -0.7693934893324155 1106 28-29 -0.8293963260571218 1106 30-31 -0.8523310527722217 1106 32-33 -0.7413995366854778 1106 34-35 -0.8006802453457311 1106 36-37 -0.8815126886014042 1106 38-39 -0.7830815273143443 1106 40-41 -0.8165297290395657 1106 42-43 -0.7902387278567318 1106 44-45 -0.8108322148412697 1106 46-47 -0.8139577256290025 1106 48-49 -0.9711088871348323 1106 50-51 -1.040945988319919 1106 52-53 -0.9389287838227958 1106 54-55 -0.8481739498460428 1106 56-57 -0.9111228211423281 1106 58-59 -0.8732893234524752 1106 60-61 -0.9959968185519301 1106 62-63 -1.1095078410926433 1106 64-65 -1.0386973932281194 1106 66-67 -0.9345819138043865 1106 68-69 -0.9579273638419323 1106 70-71 -1.0336888657957815 1106 72-73 -1.082165259655433 1106 74-75 -1.0278737007309857 1106 76-77 -1.0492075108896053 1106 78-79 -0.8752542280563631 1106 80-81 -0.8174709518380077 1106 82-83 -0.8230906954377204 1106 84-85 -0.8640476707246805 1106 86-87 -0.8934475046136328 1106 88-89 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0.9885867724600743 2201 38-39 0.9507561353756131 2201 40-41 0.9654888097327969 2201 42-43 1.075573311206412 2201 44-45 0.9981444414649303 2201 46-47 1.0271218497724632 2201 48-49 1.0671700058854938 2201 50-51 1.0176232469209978 2201 52-53 1.051664074763437 2201 54-55 1.0276266934834837 2201 56-57 1.0735953768850166 2201 58-59 1.1087182730043637 2201 60-61 1.0547729307550355 2201 62-63 0.9546996744827894 2201 64-65 -1.3001217603363813 2201 66-67 0.39853501066847485 2201 68-69 0.9520750002223934 2201 70-71 1.0683474532564574 2201 72-73 1.0669853283597064 2201 74-75 1.0203929971769767 2201 76-77 1.189784939043733 2201 78-79 0.9234982562423966 2201 80-81 0.8619351647984246 2201 82-83 0.8723952128255732 2201 84-85 0.9113027913293337 2201 86-87 0.8278682177508649 2201 88-89 0.8863393590633564 2201 90-91 0.8760924184607468 2201 92-93 0.8827517331443602 2201 94-95 1.0007186654222409 2201 96-97 0.9831490599662338 2201 98-99 0.9415345587672697 2201 100 1.2197235232002477 2202 1 0.6781340481751172 2202 2 0.6245514402926986 2202 3 0.6301787262192136 2202 4 0.3935951302408185 2202 5 0.49436967677206667 2202 6 0.49558266736266177 2202 7 0.5000676918537863 2202 8 0.5353132236771216 2202 9 0.6751761772759437 2202 10-11 0.6754602923495199 2202 12-13 0.6134065046409063 2202 14-15 0.830136898101415 2202 16-17 0.858585834455269 2202 18-19 0.807684445092832 2202 20-21 0.7767207748439446 2202 22-23 -0.7189039655468079 2202 24-25 0.523125991527877 2202 26-27 0.8049368036397766 2202 28-29 0.8610694226443485 2202 30-31 0.960062903464042 2202 32-33 0.9811695965865113 2202 34-35 0.8728303067786243 2202 36-37 -1.0467460031350413 2202 38-39 0.607693770293686 2202 40-41 0.9267094319424132 2202 42-43 0.47611190009463655 2202 44-45 0.8881124650415018 2202 46-47 0.9729619978794162 2202 48-49 1.019359524725715 2202 50-51 1.001036442604331 2202 52-53 0.9222579104041557 2202 54-55 0.8454843986770157 2202 56-57 0.8176219127495159 2202 58-59 0.9674145389843787 2202 60-61 -0.07017614664897565 2202 62-63 0.7083980533713543 2202 64-65 0.9990556443096565 2202 66-67 1.0670481286470022 2202 68-69 0.809601449359306 2202 70-71 1.0362934916946074 2202 72-73 1.1046965178188728 2202 74-75 1.1031792540597607 2202 76-77 1.2367672245029553 2202 78-79 0.9848494087526163 2202 80-81 0.882256155775309 2202 82-83 0.94837930428546 2202 84-85 0.9418237913512186 2202 86-87 0.9597739145812092 2202 88-89 0.8729288068769563 2202 90-91 0.3526547123716064 2202 92-93 0.7746542986698088 2202 94-95 1.0019532307170067 2202 96-97 1.0456273044531414 2202 98-99 1.0191659966240358 2202 100 1.2615356220156997 2203 1 0.437058338906418 2203 2 0.5060007830470497 2203 3 0.5194931603183761 2203 4 0.37658166492315104 2203 5 -0.6839516415329712 2203 6 0.03739973639298455 2203 7 0.3236916656289779 2203 8 0.417414931367901 2203 9 0.6246922856180745 2203 10-11 0.5995307060371928 2203 12-13 0.6858302420377811 2203 14-15 0.8085933767632696 2203 16-17 0.8636005554933774 2203 18-19 0.8076754525373602 2203 20-21 0.771895504468155 2203 22-23 -0.05054768013945932 2203 24-25 0.6683098553403042 2203 26-27 0.7987260614837197 2203 28-29 0.8603598313330636 2203 30-31 0.9271695856116864 2203 32-33 0.37331440379713854 2203 34-35 -0.4611996498516291 2203 36-37 -0.00583279833620054 2203 38-39 0.6977831018900744 2203 40-41 0.911446423750057 2203 42-43 0.3163159991801905 2203 44-45 0.9112156214779219 2203 46-47 1.0148683967076053 2203 48-49 0.8163671754570174 2203 50-51 0.8647436195493583 2203 52-53 1.0448353777848922 2203 54-55 0.8655379097775793 2203 56-57 0.7664953456337287 2203 58-59 0.912940965204804 2203 60-61 1.0799596252016173 2203 62-63 0.9973978671804886 2203 64-65 0.8981055426061033 2203 66-67 1.043403226947099 2203 68-69 1.1124495175717968 2203 70-71 1.122299730999373 2203 72-73 1.1350200892986138 2203 74-75 1.0437628210009464 2203 76-77 0.6701337823312272 2203 78-79 0.8191185336362352 2203 80-81 0.8211006186924692 2203 82-83 0.865492784805042 2203 84-85 0.8643559272598509 2203 86-87 0.9037872309372013 2203 88-89 0.9743172731781868 2203 90-91 0.9557763026474895 2203 92-93 0.9470429358745491 2203 94-95 1.0454611362298891 2203 96-97 1.066407110536776 2203 98-99 0.9551524343216542 2203 100 1.2516617286676741 2204 1 0.6560115479610076 2204 2 0.6208143609501064 2204 3 0.6170850800368726 2204 4 0.4215448272676312 2204 5 0.48254112130845783 2204 6 0.48226556866953985 2204 7 0.5044716643487135 2204 8 0.5059295583692105 2204 9 0.6595801524020501 2204 10-11 0.6548217856253586 2204 12-13 0.7002786838845978 2204 14-15 0.877498513989984 2204 16-17 0.8863094806862861 2204 18-19 0.8578264013528027 2204 20-21 0.8469847930520231 2204 22-23 0.935726991810931 2204 24-25 0.932977473556754 2204 26-27 0.9667858844586306 2204 28-29 0.9818722111457419 2204 30-31 1.0800418053897687 2204 32-33 1.0410781992253249 2204 34-35 1.0952543571536921 2204 36-37 1.1215903046681959 2204 38-39 1.1084987014483403 2204 40-41 1.1187497513617544 2204 42-43 -0.5697483236613294 2204 44-45 0.6881764398615076 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0.4991732088778278 2205 8 0.5215383095625299 2205 9 0.6460479640360148 2205 10-11 0.6724069681166185 2205 12-13 0.6996708038257893 2205 14-15 0.8780386717686568 2205 16-17 0.916104575927065 2205 18-19 0.8479690629526075 2205 20-21 0.8643528118224211 2205 22-23 0.8728419552482976 2205 24-25 0.9805991240662806 2205 26-27 0.9582383038743387 2205 28-29 0.931287496087208 2205 30-31 1.0144051909425897 2205 32-33 1.0627103942689686 2205 34-35 0.9941424588917585 2205 36-37 1.0976657316491796 2205 38-39 1.0571792752356046 2205 40-41 1.1019424887317726 2205 42-43 1.1097875084613662 2205 44-45 1.1126836289507693 2205 46-47 1.1163362592195867 2205 48-49 1.1662301874448389 2205 50-51 1.2014122606572712 2205 52-53 1.1728656005629716 2205 54-55 1.1144345680729444 2205 56-57 1.1780696004958884 2205 58-59 1.2135406571295206 2205 60-61 1.2431027468422897 2205 62-63 1.2631239794707412 2205 64-65 0.7430894954389586 2205 66-67 1.0782404548583528 2205 68-69 1.2230024674249265 2205 70-71 1.20904475532938 2205 72-73 1.1804134004477262 2205 74-75 1.2726925816661137 2205 76-77 1.198940783450098 2205 78-79 1.0838920077614986 2205 80-81 1.0714853559642847 2205 82-83 1.0036337041557317 2205 84-85 0.9716300743380302 2205 86-87 1.0235327733350097 2205 88-89 1.1312221380423253 2205 90-91 1.1150714048214816 2205 92-93 1.2233717641960737 2205 94-95 1.2772343376057194 2205 96-97 1.287784053597715 2205 98-99 1.3101891997408615 2205 100 1.6016277743740588 2206 1 0.4843788037606984 2206 2 0.5127330395646084 2206 3 0.4966423306109533 2206 4 0.39210402279100265 2206 5 -0.44707759417947557 2206 6 0.18760273771118818 2206 7 0.3643917228198603 2206 8 0.42373935301684185 2206 9 0.6525276790955985 2206 10-11 0.6541443472366026 2206 12-13 0.6962778933576388 2206 14-15 0.913182620115343 2206 16-17 0.9387445108440176 2206 18-19 0.900844699394618 2206 20-21 0.8752620809927691 2206 22-23 0.9364640783956659 2206 24-25 0.9207787011911179 2206 26-27 0.9461073055276046 2206 28-29 0.9250903863553646 2206 30-31 0.9693080865245634 2206 32-33 0.9796512963757138 2206 34-35 1.050292754631954 2206 36-37 1.1043613673577326 2206 38-39 1.0990616692080906 2206 40-41 1.0709448155854915 2206 42-43 1.1325584177754422 2206 44-45 1.096093813070837 2206 46-47 1.0956969395080947 2206 48-49 1.0956530244400895 2206 50-51 1.151130815388477 2206 52-53 1.122518443568083 2206 54-55 1.1533400262625477 2206 56-57 1.1960945215557501 2206 58-59 1.1830489193687086 2206 60-61 1.276363732413401 2206 62-63 1.2719049521090682 2206 64-65 1.2981649030125766 2206 66-67 1.1888619545675922 2206 68-69 1.2450402194851549 2206 70-71 1.0325152176091308 2206 72-73 1.1673427543547632 2206 74-75 1.2072737143177292 2206 76-77 1.2172164269273154 2206 78-79 1.1129523824680234 2206 80-81 1.0915986076544186 2206 82-83 1.0270222887266485 2206 84-85 1.002413024315544 2206 86-87 1.058463794733239 2206 88-89 1.1297385349142104 2206 90-91 1.1732144573032919 2206 92-93 1.1711699348953744 2206 94-95 1.2096728039628886 2206 96-97 1.273457999197685 2206 98-99 1.2964042567072411 2206 100 1.590887574439769 2207 1 0.6607334281959893 2207 2 0.6172744644525494 2207 3 0.5874485227985105 2207 4 0.36178013890681626 2207 5 0.47420248632901973 2207 6 0.4684650638916352 2207 7 0.4815003352974614 2207 8 0.5130501398872767 2207 9 0.6595178383986848 2207 10-11 0.6590796613978611 2207 12-13 0.7088788782301663 2207 14-15 0.8921966787046856 2207 16-17 0.9079159025106378 2207 18-19 0.8636078889828482 2207 20-21 0.8970778934482055 2207 22-23 0.9311255163652987 2207 24-25 0.8891872126666343 2207 26-27 0.959548625786951 2207 28-29 0.9198054275993854 2207 30-31 0.9334887178451012 2207 32-33 1.015149691486691 2207 34-35 1.1046029228564151 2207 36-37 1.0950178855614396 2207 38-39 1.076890408049188 2207 40-41 1.0826666898118518 2207 42-43 1.199565677592787 2207 44-45 1.159246032580029 2207 46-47 1.2145206873028798 2207 48-49 1.2219718784073734 2207 50-51 1.1743011246523594 2207 52-53 1.2103768475479058 2207 54-55 1.2479532371724034 2207 56-57 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2310 12-13 0.6317622563212382 2310 14-15 0.8008916046533088 2310 16-17 0.6462674128797232 2310 18-19 0.7662268992055701 2310 20-21 0.854832887653636 2310 22-23 0.911365573484801 2310 24-25 0.9200930396972709 2310 26-27 0.9655117854321702 2310 28-29 0.9613268778590012 2310 30-31 0.828371376073008 2310 32-33 0.9303181248040175 2310 34-35 0.9152571729933712 2310 36-37 0.8203260593698971 2310 38-39 0.9215266794072932 2310 40-41 1.0204741024687323 2310 42-43 1.0202328832604195 2310 44-45 1.0092531046465325 2310 46-47 0.7156401163537183 2310 48-49 0.9412990923488991 2310 50-51 0.9548346677295072 2310 52-53 1.0354209655254607 2310 54-55 1.041177858106117 2310 56-57 1.068526418060479 2310 58-59 1.0712700556539474 2310 60-61 1.0478314142233245 2310 62-63 0.5610355939639504 2310 64-65 0.7124788695244249 2310 66-67 1.0848543345602195 2310 68-69 1.1573433250247689 2310 70-71 1.1903384406615132 2310 72-73 1.1666005001138657 2310 74-75 1.153736965096357 2310 76-77 1.1922672768349614 2310 78-79 1.0337359489938152 2310 80-81 0.9557490217579669 2310 82-83 0.9588618748612774 2310 84-85 1.0586164439596146 2310 86-87 1.0544269260301142 2310 88-89 1.0478026643772296 2310 90-91 1.1152107281944446 2310 92-93 1.1621381991670248 2310 94-95 1.2130337761539707 2310 96-97 1.2271246952048465 2310 98-99 1.2109745513635204 2310 100 1.413389153392128 2311 1 0.4384680680823294 2311 2 0.37362288061931537 2311 3 0.45626407049635986 2311 4 0.32349456857808434 2311 5 0.3635875880117112 2311 6 0.44427408674344093 2311 7 0.4838472709142039 2311 8 0.49350846332720266 2311 9 0.642232029357622 2311 10-11 0.6404682303168059 2311 12-13 0.7195135294952522 2311 14-15 0.8212912928471852 2311 16-17 0.6834240639780802 2311 18-19 0.8493286081756253 2311 20-21 0.7941035894733517 2311 22-23 0.9358292227839442 2311 24-25 0.9474636384964086 2311 26-27 0.34874012571388846 2311 28-29 0.6460361459253079 2311 30-31 0.8717857304430723 2311 32-33 0.974797525372388 2311 34-35 1.0479936932476335 2311 36-37 1.0733697854799544 2311 38-39 1.077490301409945 2311 40-41 1.0219900765016448 2311 42-43 0.9944719049779565 2311 44-45 0.9819004933690181 2311 46-47 0.7331850314683805 2311 48-49 1.007707372610298 2311 50-51 1.137814916800366 2311 52-53 1.044246881324618 2311 54-55 1.084895310620844 2311 56-57 1.1846953382035679 2311 58-59 1.2113275287274021 2311 60-61 1.2616886415485737 2311 62-63 1.2551985339285423 2311 64-65 1.307163176542339 2311 66-67 1.2744214102174638 2311 68-69 1.3214071314709628 2311 70-71 1.3012220133598689 2311 72-73 1.292916956599008 2311 74-75 1.356802393907735 2311 76-77 1.2512431068592633 2311 78-79 0.9773010139991669 2311 80-81 0.9006188755248843 2311 82-83 1.0608440934396768 2311 84-85 1.1071076502259203 2311 86-87 1.149235779824469 2311 88-89 1.1644820434866432 2311 90-91 1.243479597766541 2311 92-93 1.2909614983701516 2311 94-95 1.306712998881217 2311 96-97 1.2681756972673988 2311 98-99 1.3578184999052496 2311 100 1.4755856323515815 2312 1 0.2575176351126913 2312 2 0.38287230144997153 2312 3 0.46383858604579586 2312 4 0.18147643976607952 2312 5 0.34386736813600294 2312 6 0.4251085050352472 2312 7 0.32978918084987185 2312 8 0.41237205663084353 2312 9 0.6203241569493088 2312 10-11 0.608127528854844 2312 12-13 0.6921562725888251 2312 14-15 0.8675544971707225 2312 16-17 0.8729974840251984 2312 18-19 0.8188465570923498 2312 20-21 0.8566614967575816 2312 22-23 0.906420936240643 2312 24-25 0.9180751292600249 2312 26-27 0.9376097641489594 2312 28-29 0.9745365128872976 2312 30-31 0.9550296646607634 2312 32-33 0.7649067672114072 2312 34-35 0.9134142290848644 2312 36-37 0.9903340665869322 2312 38-39 0.8073912044050147 2312 40-41 0.9464367619001806 2312 42-43 1.0082095352222353 2312 44-45 0.7186424958303803 2312 46-47 0.7946615558355461 2312 48-49 0.953209820019012 2312 50-51 0.7922326348944893 2312 52-53 1.0009703652381177 2312 54-55 0.8635623189158608 2312 56-57 0.9340553640548563 2312 58-59 1.0604612034534213 2312 60-61 1.1090075579057412 2312 62-63 1.132423125432176 2312 64-65 1.1152352005574144 2312 66-67 1.1365004953913669 2312 68-69 1.1959229531336533 2312 70-71 1.0396040594089158 2312 72-73 1.0471914552986235 2312 74-75 1.0987636449322054 2312 76-77 1.1148317582159635 2312 78-79 1.0064173440168602 2312 80-81 0.9302780809358779 2312 82-83 0.9678302610266698 2312 84-85 0.9390790412087213 2312 86-87 1.003511655926662 2312 88-89 0.986901492963618 2312 90-91 1.0443166928513286 2312 92-93 1.0309303507878127 2312 94-95 1.1259981058153983 2312 96-97 0.8396752937371978 2312 98-99 0.5541937085929192 2312 100 0.8433170962205025 2313 1 0.046613939393751025 2313 2 0.2096082862994919 2313 3 0.24661372411018334 2313 4 0.15851511364423487 2313 5 0.24870773775742805 2313 6 0.21371423907837794 2313 7 0.2199485411879678 2313 8 0.36594030972887026 2313 9 0.49970345374804737 2313 10-11 0.3382229797445788 2313 12-13 0.34381721768613005 2313 14-15 0.3914450557669866 2313 16-17 0.6807868799495935 2313 18-19 0.6702626965262866 2313 20-21 0.5965582184582203 2313 22-23 0.5831098690624614 2313 24-25 0.47849754865075766 2313 26-27 0.7117671833655379 2313 28-29 0.5205981874386367 2313 30-31 0.7802425161943276 2313 32-33 0.857221839111709 2313 34-35 0.8683125355645274 2313 36-37 0.924978207716947 2313 38-39 0.9286312968614823 2313 40-41 0.9593643406887011 2313 42-43 0.9352756529796125 2313 44-45 0.8367713276859803 2313 46-47 0.8656637075608913 2313 48-49 0.926101084207474 2313 50-51 0.9192741300319582 2313 52-53 0.8781749545235868 2313 54-55 0.8101451517827556 2313 56-57 0.6351641189653634 2313 58-59 0.7690115152045607 2313 60-61 0.5138319291641338 2313 62-63 0.8773753001337425 2313 64-65 0.9580562764105736 2313 66-67 0.9117417715087655 2313 68-69 1.0278565436216027 2313 70-71 1.020585472897082 2313 72-73 0.9995552545474808 2313 74-75 1.086405350308965 2313 76-77 1.0422756849936619 2313 78-79 0.8866158263457677 2313 80-81 0.7256037317264941 2313 82-83 0.8168569681688993 2313 84-85 0.9037826502848887 2313 86-87 0.9426012022614323 2313 88-89 0.9254019032943859 2313 90-91 0.7321410014634928 2313 92-93 0.905825430254005 2313 94-95 1.0315994823997485 2313 96-97 1.1146410017661061 2313 98-99 1.1077744651793786 2313 100 1.2495262719147036 2314 1 -0.2939908193345353 2314 2 0.05103503293429412 2314 3 0.2758563310549462 2314 4 0.23285601716200688 2314 5 0.2995039558416792 2314 6 0.39040882592042436 2314 7 0.4498638732096438 2314 8 0.4139200043193938 2314 9 0.4633015079712024 2314 10-11 0.5372143470420241 2314 12-13 0.37262677729531646 2314 14-15 0.7079520539677873 2314 16-17 0.8007108292067997 2314 18-19 0.777237087584929 2314 20-21 0.8264337060505937 2314 22-23 0.8870042313437168 2314 24-25 0.786569573138209 2314 26-27 0.7111231257077577 2314 28-29 0.722603371941581 2314 30-31 0.7352532899144038 2314 32-33 0.7942097679802771 2314 34-35 0.820205821403377 2314 36-37 0.8266562386992007 2314 38-39 0.5711764090823479 2314 40-41 0.5965634658003651 2314 42-43 0.6950958164483865 2314 44-45 0.8287587603039981 2314 46-47 0.7978337042091752 2314 48-49 0.8301957703154557 2314 50-51 0.7757841342175595 2314 52-53 0.7982766184404042 2314 54-55 0.8182308018980677 2314 56-57 0.7434866738317112 2314 58-59 0.8295160772087584 2314 60-61 0.9702871697578317 2314 62-63 0.8570107322093463 2314 64-65 0.9058635649762934 2314 66-67 0.5546024053273229 2314 68-69 0.5684834724272321 2314 70-71 0.7423246667100756 2314 72-73 0.8556406075058263 2314 74-75 0.9009612373179579 2314 76-77 0.7962341682257161 2314 78-79 0.7182017109785406 2314 80-81 0.7924825123734252 2314 82-83 0.8410160430328659 2314 84-85 0.8127579180198623 2314 86-87 0.9025011910626581 2314 88-89 0.901320400955484 2314 90-91 1.0518559855282952 2314 92-93 0.9125693941245885 2314 94-95 0.9168262637582458 2314 96-97 0.8349505430403248 2314 98-99 1.0143463592650726 2314 100 1.0298104017588479 2315 1 0.3925412092689058 2315 2 0.42738949871045406 2315 3 0.4565164218045581 2315 4 0.33018481628130303 2315 5 0.37023426484118005 2315 6 0.44334446817485684 2315 7 0.35092212871326467 2315 8 0.22271860093441376 2315 9 0.4557018810311746 2315 10-11 0.5375673574922786 2315 12-13 0.3630799781956213 2315 14-15 0.5622810360236699 2315 16-17 0.7365862461225561 2315 18-19 0.7374340874248588 2315 20-21 0.45994658674653266 2315 22-23 0.8117409333236836 2315 24-25 0.8052278802560764 2315 26-27 0.7952033140017747 2315 28-29 0.8111585648906825 2315 30-31 0.8088525999821243 2315 32-33 0.7406201682520717 2315 34-35 0.831742872596223 2315 36-37 0.8527483864727614 2315 38-39 0.9219256732525807 2315 40-41 0.9106127100435799 2315 42-43 0.9792018527025022 2315 44-45 1.0051800714366692 2315 46-47 0.9232163193530525 2315 48-49 0.8308834158888772 2315 50-51 0.9138661420772962 2315 52-53 0.9068364174714958 2315 54-55 0.6945462711467556 2315 56-57 0.6801211959262048 2315 58-59 0.713117771985587 2315 60-61 0.8641478096438888 2315 62-63 0.9612135370192689 2315 64-65 1.0593053045725966 2315 66-67 1.0143982105259894 2315 68-69 1.0885750550574045 2315 70-71 0.9886818602014529 2315 72-73 1.0634439147380306 2315 74-75 0.5018624041920603 2315 76-77 0.5173873547524366 2315 78-79 0.7946414382723432 2315 80-81 0.8077109626065067 2315 82-83 0.748722900214922 2315 84-85 0.8022980693345616 2315 86-87 0.827184604594084 2315 88-89 0.838911460909685 2315 90-91 0.9241864588440514 2315 92-93 0.943345412771059 2315 94-95 0.9347197489289485 2315 96-97 1.1106372085963052 2315 98-99 1.00313965561908 2315 100 1.1024252026356365 2316 1 0.2937987134945885 2316 2 0.07889228668814496 2316 3 0.2463454841244399 2316 4 0.05227427766084958 2316 5 0.282095636616873 2316 6 0.36228911661767427 2316 7 0.3895668671744019 2316 8 0.4622709702297456 2316 9 0.629196826404943 2316 10-11 0.6051244122739305 2316 12-13 0.40645027409039614 2316 14-15 0.5463958773906583 2316 16-17 0.8109250323646506 2316 18-19 0.7885818211001308 2316 20-21 0.7183716383937409 2316 22-23 0.7504515664957623 2316 24-25 0.48229117803540333 2316 26-27 0.7773494092139686 2316 28-29 0.9235130946258749 2316 30-31 0.5742269531777708 2316 32-33 0.702274546445615 2316 34-35 0.7801203570049182 2316 36-37 0.8273376373778021 2316 38-39 0.7071813673975029 2316 40-41 0.7004502722954626 2316 42-43 0.6850608963063394 2316 44-45 0.5275029166634297 2316 46-47 0.6818295837802779 2316 48-49 0.8333815863797085 2316 50-51 0.8437182270701413 2316 52-53 0.8791234010803848 2316 54-55 0.7826003048097263 2316 56-57 0.8847493684246359 2316 58-59 0.7453867519289901 2316 60-61 0.8446048469399727 2316 62-63 0.8244650119598518 2316 64-65 0.9261890837880742 2316 66-67 0.9037724050562446 2316 68-69 0.8878074707218815 2316 70-71 0.9411008598970767 2316 72-73 0.9149420075151937 2316 74-75 0.9217278786015939 2316 76-77 0.7933890515007604 2316 78-79 0.537739493487905 2316 80-81 0.5446554718123409 2316 82-83 0.45908407446838595 2316 84-85 0.6108451961956405 2316 86-87 0.6523268367895589 2316 88-89 0.5951653870687466 2316 90-91 0.5710608314681132 2316 92-93 0.3663421002261309 2316 94-95 0.4136318223942652 2316 96-97 0.5980856423421699 2316 98-99 0.7760736864325501 2316 100 0.42595932864834296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 22.0 13 104.0 14 442.0 15 1377.0 16 3303.0 17 6701.0 18 11122.0 19 16445.0 20 23173.0 21 31941.0 22 40209.0 23 43144.0 24 44635.0 25 49078.0 26 56793.0 27 69891.0 28 85951.0 29 107296.0 30 132874.0 31 166021.0 32 210968.0 33 279237.0 34 400364.0 35 562776.0 36 887134.0 37 1396218.0 38 1270364.0 39 200883.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60433093826606 19.896048426084977 13.448265283707938 24.051355351941027 2 15.376753406788735 19.874509816782364 41.238307546874346 23.510429229554553 3 18.91538636235065 25.540048061116817 29.46426858473867 26.080296991793862 4 12.721711264120605 16.227592262378785 38.05129796625899 32.999398507241615 5 13.141476940584424 37.6328875122264 34.47895665890791 14.74667888828127 6 30.941209169162505 36.73579585749576 17.978399553331688 14.34459542001005 7 27.955792570942826 32.00804731445722 22.152251595164135 17.883908519435813 8 26.154971275652887 36.23024406881381 19.59903156275698 18.015753092776322 9 26.54044516718245 16.115732906250273 20.90218126245224 36.44164066411504 10-11 25.639964633706008 26.252487922364686 27.501392974162993 20.60615446976631 12-13 25.478016261415743 23.672731834217842 28.102692717945523 22.74655918642089 14-15 22.91274645815113 25.153544546263166 25.891524183629855 26.042184811955842 16-17 22.057045839739317 27.953784756292734 26.85893529911794 23.130234104850008 18-19 21.793388029977937 27.36850217718007 28.83940847603954 21.998701316802453 20-21 22.82513982163436 26.82108373162904 28.466968184224147 21.886808262512453 22-23 22.853356202401088 26.55596430916859 28.305513582471715 22.28516590595861 24-25 22.282524524097592 26.44432621749774 28.861378577031292 22.411770681373376 26-27 22.321723728104296 26.674399224984157 28.297383796816767 22.706493250094777 28-29 21.961646149242227 27.22445365439721 28.418719433956852 22.395180762403715 30-31 22.950263756224132 26.75156901317154 28.446669786095054 21.85149744450928 32-33 22.840905028193163 26.74427452998551 28.139525234445546 22.27529520737578 34-35 22.75530466835746 26.80030854817194 28.469916307210454 21.974470476260144 36-37 22.591444601798827 26.78278468704575 28.182136986229466 22.443633724925952 38-39 22.57140659398694 26.896140679243995 28.368694872908883 22.163757853860183 40-41 22.529568372598224 26.245890730826797 28.802041450817985 22.42249944575699 42-43 22.363342132250047 26.587658428873567 28.225493311609878 22.82350612726651 44-45 22.204109069876097 27.057564729715523 28.332512542681528 22.405813657726853 46-47 22.30014707263141 26.763495974260852 27.87598337140329 23.060373581704443 48-49 22.611346865412504 26.716704864630152 28.0131289596754 22.65881931028195 50-51 22.542255207852023 26.741524461646808 27.90677594541387 22.809444385087293 52-53 22.239454371516302 27.114035274010988 27.661767803685134 22.98474255078758 54-55 22.19827921968771 26.93299157157113 27.85158290305626 23.017146305684903 56-57 22.661445670028584 26.788689425719063 27.408453210056155 23.141411694196194 58-59 22.02149014825785 27.05080570427112 27.96378360818221 22.963920539288814 60-61 22.516397466923834 27.25411670791929 27.59871030753675 22.630775517620123 62-63 22.643001009608913 27.055993858062138 27.298295077676922 23.00271005465203 64-65 22.08934256721722 26.914601337249845 27.557275850060435 23.438780245472497 66-67 22.197747294871053 26.740690332417373 27.512848311373585 23.548714061337996 68-69 21.96305649619763 26.76120478426734 27.743290075146632 23.532448644388403 70-71 22.10874554545778 26.572056983358827 27.738880030345275 23.580317440838115 72-73 22.219002233494646 26.800680720017183 27.546595255646917 23.433721790841254 74-75 22.365160195622412 26.956937676604937 27.315495107307232 23.362407020465422 76-77 22.09294753557496 26.70819928768884 27.29276487318201 23.906088303554192 78-79 21.98647463294621 26.627339655536186 27.526117032612763 23.86006867890484 80-81 21.757811020360577 26.989582354593487 27.569209512719972 23.683397112325967 82-83 22.327695491585725 26.57938690972607 27.94515251470292 23.147765083985284 84-85 22.491446130927724 26.803916855818393 27.268709204545548 23.435927808708335 86-87 21.633876940637712 27.228654451533558 27.495231031532004 23.642237576296726 88-89 21.966367410754685 26.39358050245489 27.648671628493286 23.991380458297133 90-91 22.093271346571484 26.38179279004001 27.639604537037226 23.885331326351277 92-93 22.102332159859213 26.503241510029156 27.578602137811153 23.81582419230048 94-95 21.513198948118493 26.523321708626856 27.749910006240114 24.213569337014537 96-97 21.582826952011754 26.369820474009487 27.70517001302094 24.342182560957824 98-99 21.266601379429336 26.57951842817286 27.56055730217959 24.593322890218214 100 21.50038054496664 26.419399919954333 27.417428303359948 24.662791231719076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1107.0 1 756.0 2 825.5 3 1204.5 4 914.0 5 734.5 6 1076.5 7 1903.5 8 2946.5 9 3517.5 10 3363.0 11 3150.5 12 3411.5 13 3833.5 14 4336.0 15 4955.5 16 5545.5 17 6158.5 18 6735.0 19 7281.0 20 7939.0 21 8870.5 22 10034.0 23 11516.5 24 13678.0 25 16643.0 26 20662.0 27 25504.0 28 31409.5 29 40388.5 30 52250.5 31 64975.5 32 80566.5 33 96997.5 34 112735.5 35 129088.0 36 146131.5 37 167897.5 38 182492.0 39 186600.0 40 186141.0 41 183158.5 42 188161.5 43 198640.5 44 208757.5 45 226897.0 46 245284.5 47 259359.0 48 271547.5 49 273510.0 50 268448.5 51 265738.0 52 259370.0 53 244554.0 54 228407.5 55 203481.5 56 177872.5 57 152204.5 58 126820.5 59 111053.0 60 89703.5 61 66789.5 62 51598.5 63 40885.5 64 30131.0 65 19271.0 66 13110.0 67 9706.0 68 7453.5 69 5567.5 70 4187.5 71 3190.5 72 2374.5 73 1774.0 74 1329.5 75 903.5 76 583.0 77 353.5 78 203.5 79 128.0 80 88.0 81 57.0 82 34.0 83 25.5 84 13.5 85 8.5 86 10.0 87 6.5 88 4.5 89 3.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04528993676298294 2 0.0 3 0.0 4 0.03256546502942944 5 0.0 6 0.0 7 8.690683013896074E-4 8 0.0 9 0.011248695372703222 10-11 3.771428477728485E-4 12-13 0.0 14-15 1.721739087658656E-4 16-17 3.443478175317312E-4 18-19 0.0 20-21 1.639751512055863E-5 22-23 0.0 24-25 7.296894228648591E-4 26-27 4.755279384962003E-4 28-29 1.6397515120558632E-4 30-31 0.0023858384500412807 32-33 0.014872546214346678 34-35 0.025317763346142524 36-37 0.030220620367189557 38-39 0.021710310019619625 40-41 0.0012626086642830146 42-43 1.1478260584391041E-4 44-45 0.011576645675114393 46-47 0.018980123752046614 48-49 0.0034516769328775915 50-51 0.01222434752237646 52-53 0.011634036978036348 54-55 0.010986335130774282 56-57 5.329192414181555E-4 58-59 0.008657887983654957 60-61 0.004706086839600328 62-63 0.001377391270126925 64-65 0.036763228900292454 66-67 0.06251552639712978 68-69 0.06231875621568307 70-71 0.0541445949280846 72-73 0.047233042304769136 74-75 0.036910806536377475 76-77 0.03266385012015279 78-79 0.016061366060587178 80-81 0.0013445962398858076 82-83 0.03963279404639021 84-85 0.05394782474663789 86-87 0.05925242088813861 88-89 0.07256720316603221 90-91 0.050791303085930356 92-93 0.04072322880190736 94-95 0.01377391270126925 96-97 0.003796024750409323 98-99 0.021743105049860743 100 0.03222111721189771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6098485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.38182133039467 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47670264696725 52.094264537094915 2 13.56966374161984 18.01557988019486 3 3.979911046860723 7.925812320707175 4 1.5751083025942958 4.182342316753431 5 0.7664244544911996 2.5438325600640006 6 0.4254579948404602 1.6945605958252445 7 0.28498110128916687 1.3242295183821609 8 0.1885317160633548 1.0012062952664254 9 0.1372146928803966 0.8197705104022318 >10 0.5553226989245059 6.186724391082012 >50 0.026339652363173215 1.201331608405711 >100 0.013168929864265462 1.5788757965269031 >500 7.624652622499719E-4 0.35287530719359006 >1k 3.2257969798738515E-4 0.35927838625748737 >5k 2.9325427088256418E-5 0.15748602864808142 >10k+ 5.8650854176512835E-5 0.5618299471955843 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15577 0.2554240930329418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13284 0.21782459086150086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8090 0.13265589732531932 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.279503024111726E-5 0.0 0.0 0.0 6 3.279503024111726E-5 3.279503024111726E-5 0.0 0.0 1.639751512055863E-5 7 3.279503024111726E-5 3.279503024111726E-5 0.0 0.0 1.639751512055863E-5 8 3.279503024111726E-5 3.279503024111726E-5 0.0 0.0 1.639751512055863E-5 9 3.279503024111726E-5 3.279503024111726E-5 0.0 3.279503024111726E-5 3.279503024111726E-5 10-11 4.919254536167589E-5 3.279503024111726E-5 0.0 4.919254536167589E-5 3.279503024111726E-5 12-13 6.559006048223452E-5 4.099378780139658E-5 0.0 4.919254536167589E-5 3.279503024111726E-5 14-15 6.559006048223452E-5 4.919254536167589E-5 0.0 5.7391302921955206E-5 4.919254536167589E-5 16-17 6.559006048223452E-5 4.919254536167589E-5 0.0 6.559006048223452E-5 5.7391302921955206E-5 18-19 6.559006048223452E-5 4.919254536167589E-5 0.0 6.559006048223452E-5 6.559006048223452E-5 20-21 6.559006048223452E-5 4.919254536167589E-5 0.0 1.2298136340418972E-4 7.378881804251383E-5 22-23 6.559006048223452E-5 4.919254536167589E-5 0.0 2.131676965672622E-4 1.1478260584391041E-4 24-25 6.559006048223452E-5 4.919254536167589E-5 0.0 5.165217262975969E-4 1.4757763608502767E-4 26-27 6.559006048223452E-5 4.919254536167589E-5 0.0 0.0013445962398858078 1.4757763608502767E-4 28-29 6.559006048223452E-5 4.919254536167589E-5 0.0 0.004369937779628874 1.4757763608502767E-4 30-31 6.559006048223452E-5 4.919254536167589E-5 0.0 0.015979378484984385 1.55776393645307E-4 32-33 6.559006048223452E-5 4.919254536167589E-5 0.0 0.04720844603208829 1.6397515120558632E-4 34-35 6.559006048223452E-5 4.919254536167589E-5 0.0 0.09381018400471593 1.8037266632614494E-4 36-37 6.559006048223452E-5 4.919254536167589E-5 0.0 0.16987825664898742 1.9677018144670356E-4 38-39 6.559006048223452E-5 4.919254536167589E-5 0.0 0.28354583146469986 1.9677018144670356E-4 40-41 9.838509072335178E-5 4.919254536167589E-5 0.0 0.4101592444680933 2.0496893900698288E-4 42-43 9.838509072335178E-5 4.919254536167589E-5 0.0 0.5301890551505825 2.131676965672622E-4 44-45 1.1478260584391041E-4 4.919254536167589E-5 0.0 0.6699286790079831 2.131676965672622E-4 46-47 1.3118012096446905E-4 4.919254536167589E-5 0.0 0.8300832091904793 2.131676965672622E-4 48-49 1.3118012096446905E-4 4.919254536167589E-5 0.0 1.0047659377697904 2.131676965672622E-4 50-51 1.3118012096446905E-4 4.919254536167589E-5 0.0 1.1866225792143457 2.131676965672622E-4 52-53 1.3118012096446905E-4 4.919254536167589E-5 0.0 1.36169064939899 2.2136645412754153E-4 54-55 1.3118012096446905E-4 4.919254536167589E-5 0.0 1.5575425699989425 2.2956521168782083E-4 56-57 1.3118012096446905E-4 4.919254536167589E-5 0.0 1.7849515084484098 2.2956521168782083E-4 58-59 1.4757763608502767E-4 4.919254536167589E-5 0.0 2.042417092113861 2.3776396924810012E-4 60-61 1.55776393645307E-4 4.919254536167589E-5 0.0 2.295258576515315 2.4596272680837945E-4 62-63 1.8037266632614494E-4 4.919254536167589E-5 0.0 2.5514205577286817 2.4596272680837945E-4 64-65 1.8037266632614494E-4 4.919254536167589E-5 0.0 2.8122558307514076 2.5416148436865877E-4 66-67 1.8037266632614494E-4 4.919254536167589E-5 0.0 3.109042655675959 2.705589994892174E-4 68-69 1.8857142388642423E-4 6.559006048223452E-5 0.0 3.428540039042484 2.7875775704949674E-4 70-71 2.131676965672622E-4 8.198757560279316E-5 0.0 3.7620409003219653 2.7875775704949674E-4 72-73 2.9515527217005534E-4 9.838509072335178E-5 0.0 4.088523625129848 3.11552787290614E-4 74-75 2.9515527217005534E-4 9.838509072335178E-5 0.0 4.415695045572793 3.11552787290614E-4 76-77 2.9515527217005534E-4 9.838509072335178E-5 0.0 4.7828846016674635 3.11552787290614E-4 78-79 3.197515448508933E-4 9.838509072335178E-5 0.0 5.181024467552188 3.11552787290614E-4 80-81 3.5254657509201055E-4 9.838509072335178E-5 0.0 5.590404830052054 3.11552787290614E-4 82-83 3.6074533265228987E-4 9.838509072335178E-5 0.0 6.0059998507826124 3.2795030241117263E-4 84-85 3.771428477728485E-4 9.838509072335178E-5 0.0 6.423603567115439 3.2795030241117263E-4 86-87 3.771428477728485E-4 9.838509072335178E-5 0.0 6.860736723956851 3.2795030241117263E-4 88 3.771428477728485E-4 9.838509072335178E-5 0.0 7.220727770913596 3.2795030241117263E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9575 0.0 48.665176 1 TACCTGG 4670 0.0 40.555584 2 GTATCAA 16370 0.0 38.632874 1 CAACGCA 18955 0.0 32.39773 5 ATCAACG 19050 0.0 32.22181 3 ACCTGGG 5795 0.0 32.036522 3 GTACCTG 6090 0.0 31.50114 1 GTACTAG 3445 0.0 30.846268 1 TATCAAC 20870 0.0 30.107277 2 AACGCAG 21235 0.0 29.605108 6 TCAACGC 21215 0.0 29.199408 4 GTACATG 22080 0.0 28.897758 1 TACATGG 22170 0.0 28.214663 2 ACATGGG 21910 0.0 27.372217 3 ACGCAGA 23415 0.0 26.266865 7 CGCAGAG 24240 0.0 25.314734 8 TGGGGGG 12100 0.0 25.16242 6 CATGGGG 16725 0.0 25.066887 4 CCTGGGG 5575 0.0 24.870176 4 CTGTGCG 4370 0.0 24.836666 9 >>END_MODULE