FastQCFastQC Report
Mon 6 Jun 2016
ERR1211207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11176729
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316050.28277504089076505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248770.22257853796043545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151890.13589843683245786No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211800.044.0135961
GTATCAA366750.039.083021
GGGCACC125050.038.10872794
GTACTAG124300.037.2900921
GCATCGT117700.036.901788
CAACGCA399150.033.5999645
ATCAACG407050.033.086743
TAGGCAT153100.032.9456255
TACCTGG52400.032.867782
CTGTGCG167950.031.682419
TATCAAC452800.031.3641382
TCTAACG63650.030.6071222
CTAACGC64150.030.3375663
TAGTACT179350.030.0630684
CTAGGCA172350.030.0021694
ACTGTGC184000.029.9151278
ACCTGGG57050.029.5812053
GTGGTAT71100.029.4522691
AACGCAG470550.029.421476
TAACGCC66250.029.3755284
Produced by FastQC (version 0.11.5)