##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11176729 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5233664518483 31.0 31.0 34.0 30.0 34.0 2 31.5415884200109 31.0 31.0 34.0 28.0 34.0 3 31.605621286872037 33.0 31.0 34.0 28.0 34.0 4 35.2074611453852 37.0 35.0 37.0 32.0 37.0 5 35.27218124372524 37.0 35.0 37.0 33.0 37.0 6 35.33349336822965 37.0 35.0 37.0 32.0 37.0 7 35.252375449024484 37.0 35.0 37.0 32.0 37.0 8 35.26463252352276 37.0 35.0 37.0 32.0 37.0 9 36.83288348496237 39.0 37.0 39.0 32.0 39.0 10-11 36.79180107167312 39.0 37.0 39.0 32.0 39.0 12-13 36.67431643014696 39.0 37.0 39.0 32.0 39.0 14-15 37.86840613206243 40.0 38.0 41.0 32.0 41.0 16-17 37.85243920649771 40.0 37.5 41.0 32.0 41.0 18-19 37.8726414946627 40.0 38.0 41.0 32.0 41.0 20-21 37.843193925521504 40.0 37.5 41.0 32.0 41.0 22-23 37.728565710057026 40.0 37.0 41.0 32.0 41.0 24-25 37.625647584369275 40.0 37.0 41.0 32.0 41.0 26-27 37.472853819753524 40.0 37.0 41.0 31.5 41.0 28-29 37.36998530607658 40.0 36.5 41.0 31.0 41.0 30-31 37.23059519471215 40.0 36.0 41.0 31.0 41.0 32-33 37.06411433076708 39.0 36.0 41.0 31.0 41.0 34-35 36.92986736101412 39.0 36.0 41.0 30.0 41.0 36-37 36.83041205526232 39.0 35.0 41.0 30.0 41.0 38-39 36.682984484995565 39.0 35.0 41.0 30.0 41.0 40-41 36.51278768591419 39.0 35.0 41.0 30.0 41.0 42-43 36.30306201393986 39.0 35.0 40.0 29.0 41.0 44-45 36.053818831967746 38.5 35.0 40.0 28.0 41.0 46-47 35.80155553561333 38.0 35.0 40.0 27.5 41.0 48-49 35.569668639187725 38.0 34.0 40.0 27.0 41.0 50-51 34.86252176285208 37.5 33.5 39.5 26.0 40.5 52-53 34.941158947309184 38.0 33.5 39.5 26.5 40.5 54-55 35.39229894542491 38.0 34.0 40.0 27.0 41.0 56-57 35.31269300705063 38.0 34.0 40.0 27.0 41.0 58-59 35.063807443125796 37.0 34.0 40.0 26.0 41.0 60-61 34.83726558101212 37.0 34.0 40.0 26.0 41.0 62-63 34.52737724069359 36.0 33.5 40.0 26.0 41.0 64-65 34.27440242131665 36.0 33.0 39.0 26.0 41.0 66-67 34.113713591874685 35.0 33.0 39.0 26.0 41.0 68-69 33.833083811909546 35.0 33.0 39.0 26.0 41.0 70-71 33.58725101950669 35.0 33.0 38.0 25.0 40.5 72-73 33.30030839076442 35.0 33.0 37.0 25.0 40.0 74-75 32.99378735943226 35.0 32.0 37.0 24.5 39.5 76-77 32.70311300381355 35.0 32.0 36.0 24.0 39.0 78-79 32.39147218296158 35.0 32.0 36.0 24.0 39.0 80-81 32.125109591545076 35.0 32.0 36.0 23.0 38.0 82-83 31.847373323626258 35.0 31.0 35.0 23.0 37.0 84-85 31.621443536834434 35.0 31.0 35.0 22.5 37.0 86-87 31.41527288529587 35.0 31.0 35.0 20.5 36.5 88-89 31.224678034154714 34.0 31.0 35.0 20.0 36.0 90-91 30.99967351807492 34.0 31.0 35.0 20.0 36.0 92-93 30.794848072275887 34.0 30.0 35.0 19.5 35.5 94-95 30.644428436978295 34.0 30.0 35.0 19.0 35.0 96-97 30.46360075474676 34.0 30.0 35.0 18.5 35.0 98-99 30.236345714385667 34.0 29.0 35.0 18.0 35.0 100 29.051525540254218 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4756705821076288 1101 2 -0.4065336224052203 1101 3 -0.44226892669032836 1101 4 -0.32276337074144834 1101 5 -0.33281526508675796 1101 6 -0.35912933613204245 1101 7 -0.3858983806824199 1101 8 -0.31014945295664376 1101 9 -0.5050504935661522 1101 10-11 -0.5416428661504824 1101 12-13 -0.4972684960677256 1101 14-15 -0.6873518454929339 1101 16-17 -0.7192365894866057 1101 18-19 -0.6541256172722996 1101 20-21 -0.7224685705598048 1101 22-23 -0.77794698667023 1101 24-25 -0.836413775458503 1101 26-27 -0.7888755465874482 1101 28-29 -0.8174964831499878 1101 30-31 -0.8759576408850407 1101 32-33 -0.8065360877789445 1101 34-35 -0.8306025283790888 1101 36-37 -0.9013096399657243 1101 38-39 -0.9373764293514242 1101 40-41 -0.9600499573158956 1101 42-43 -0.9707730764039866 1101 44-45 -0.9577894645641436 1101 46-47 -1.1026353541158898 1101 48-49 -1.1351693776274558 1101 50-51 -1.1784798937893513 1101 52-53 -1.056122401107153 1101 54-55 -0.9278654577933381 1101 56-57 -0.8853105685889702 1101 58-59 -0.8892316114919367 1101 60-61 -0.8506519676322952 1101 62-63 -0.8932193247792242 1101 64-65 -0.8601228836457366 1101 66-67 -0.8787628971956423 1101 68-69 -0.9516327058008827 1101 70-71 -0.9781582224125742 1101 72-73 -0.9659331221843317 1101 74-75 -0.9623283989797571 1101 76-77 -1.0077980429610776 1101 78-79 -0.9797297778406069 1101 80-81 -1.076607700590742 1101 82-83 -1.1036901087267488 1101 84-85 -1.1958435702375105 1101 86-87 -1.1681618676286085 1101 88-89 -1.2565134141418675 1101 90-91 -1.3271494038235865 1101 92-93 -1.4363506464519062 1101 94-95 -1.4676395209440045 1101 96-97 -1.540520369572448 1101 98-99 -1.547432430481873 1101 100 -1.6616564001011334 1102 1 -0.4566783968565318 1102 2 -0.4702208947786346 1102 3 -0.42539893780023164 1102 4 -0.2900129538526599 1102 5 -0.29352045418701067 1102 6 -0.38038732979256906 1102 7 -0.35112962622758204 1102 8 -0.39198661653581723 1102 9 -0.5516251141216912 1102 10-11 -0.5310946853001042 1102 12-13 -0.5098501858032236 1102 14-15 -0.6917474725686006 1102 16-17 -0.7242295072412404 1102 18-19 -0.6901335875455459 1102 20-21 -0.7512716252123184 1102 22-23 -0.7466050252065912 1102 24-25 -0.7521271694328746 1102 26-27 -0.8044147396161492 1102 28-29 -0.866590104573369 1102 30-31 -0.835791874117831 1102 32-33 -0.887833572602915 1102 34-35 -0.9648801405731362 1102 36-37 -0.9270162308372321 1102 38-39 -1.0095457394742624 1102 40-41 -1.0526149780470533 1102 42-43 -1.008408614061409 1102 44-45 -1.2179277388490277 1102 46-47 -1.177174111568931 1102 48-49 -1.188917491338998 1102 50-51 -1.0873003638457774 1102 52-53 -1.0721791975224235 1102 54-55 -0.8729064773755582 1102 56-57 -0.8251719288243251 1102 58-59 -0.9088506238547964 1102 60-61 -0.786250416852802 1102 62-63 -0.9065693770853471 1102 64-65 -0.8976714586181558 1102 66-67 -0.9514710896405205 1102 68-69 -0.9384198110142066 1102 70-71 -0.933109973367003 1102 72-73 -0.9657700879158213 1102 74-75 -1.015486683076503 1102 76-77 -1.1093379518805904 1102 78-79 -1.1026866641179751 1102 80-81 -1.149126439661714 1102 82-83 -1.1400306606991109 1102 84-85 -1.0741195087687778 1102 86-87 -1.0474088976193592 1102 88-89 -1.1388526667848033 1102 90-91 -1.176532632780038 1102 92-93 -1.2186511934197313 1102 94-95 -1.1993221320236884 1102 96-97 -1.219749798878631 1102 98-99 -1.4131156757959857 1102 100 -1.4959857325678811 1103 1 -0.4766920331875646 1103 2 -0.42150569487018785 1103 3 -0.5279129879224378 1103 4 -0.38858187046130155 1103 5 -0.3608236979290922 1103 6 -0.34198518689059654 1103 7 -0.35556674985610925 1103 8 -0.3517726854412615 1103 9 -0.5371559270002706 1103 10-11 -0.5229406607704874 1103 12-13 -0.6012887803727622 1103 14-15 -0.7253494404388192 1103 16-17 -0.6888998301984017 1103 18-19 -0.6076662309853305 1103 20-21 -0.7501418932868589 1103 22-23 -0.7774649397169213 1103 24-25 -0.7313218845701215 1103 26-27 -0.7275196721601702 1103 28-29 -0.8134284932376801 1103 30-31 -0.7862456324382734 1103 32-33 -0.863712830317148 1103 34-35 -0.9144079862068466 1103 36-37 -0.8762998534276747 1103 38-39 -0.8883741609392644 1103 40-41 -0.963962807697655 1103 42-43 -1.0710985245160316 1103 44-45 -1.0547695804499284 1103 46-47 -1.0418870672143044 1103 48-49 -1.1395319718200057 1103 50-51 -1.1796347797622886 1103 52-53 -1.0878510135684323 1103 54-55 -0.9078601818822278 1103 56-57 -0.8003950383415486 1103 58-59 -0.7763544972099865 1103 60-61 -0.8027128943669055 1103 62-63 -0.7621806190105787 1103 64-65 -0.6712631183478805 1103 66-67 -0.7837494681687218 1103 68-69 -0.7320240730083043 1103 70-71 -0.796379733880265 1103 72-73 -0.7702182685371142 1103 74-75 -0.7538154852707422 1103 76-77 -0.7424383588788857 1103 78-79 -0.8479560700215778 1103 80-81 -0.9183800816515344 1103 82-83 -0.9005663640079398 1103 84-85 -0.9622660130016207 1103 86-87 -1.0428739324837153 1103 88-89 -1.0186799214371938 1103 90-91 -1.1444161721694854 1103 92-93 -1.1665697775162513 1103 94-95 -1.1217448954933218 1103 96-97 -1.1930640871482687 1103 98-99 -1.2337654154118916 1103 100 -1.2356803582066576 1104 1 -0.5322882655552093 1104 2 -0.5078641940084658 1104 3 -0.5209663896929442 1104 4 -0.3276372422411029 1104 5 -0.32776984558466893 1104 6 -0.40906029047044257 1104 7 -0.4319370264959801 1104 8 -0.372321108995763 1104 9 -0.5255360428394837 1104 10-11 -0.512184460059828 1104 12-13 -0.44600932650173064 1104 14-15 -0.7067379840963 1104 16-17 -0.7761170064099332 1104 18-19 -0.8073284257058546 1104 20-21 -0.7412553657390575 1104 22-23 -0.6765928705855302 1104 24-25 -0.7199595367796192 1104 26-27 -0.7903221251179602 1104 28-29 -0.8391906411068604 1104 30-31 -0.8114503202426917 1104 32-33 -0.9170385456136785 1104 34-35 -1.04010375786266 1104 36-37 -0.9960905618425144 1104 38-39 -0.9606214776255584 1104 40-41 -0.9648826042885688 1104 42-43 -0.9259122184586914 1104 44-45 -0.9400758226092094 1104 46-47 -1.0313528993179446 1104 48-49 -0.9775936612302232 1104 50-51 -1.0383473565736736 1104 52-53 -0.9008339255525684 1104 54-55 -0.7946298297211669 1104 56-57 -0.8336721333746837 1104 58-59 -0.8284901550040971 1104 60-61 -0.8793386631065019 1104 62-63 -0.8560040432638942 1104 64-65 -0.8024903106507466 1104 66-67 -0.7975384068293394 1104 68-69 -0.8707582022721212 1104 70-71 -0.8537249440083343 1104 72-73 -0.9045902164724637 1104 74-75 -0.9125781675706577 1104 76-77 -0.896225703842866 1104 78-79 -0.8917732571636989 1104 80-81 -0.9600126528057089 1104 82-83 -1.0649696476905284 1104 84-85 -1.0336053815222748 1104 86-87 -1.0153842091152434 1104 88-89 -1.132196012558257 1104 90-91 -1.1560947560990975 1104 92-93 -1.186550227614564 1104 94-95 -1.1039618276439782 1104 96-97 -1.328011518105118 1104 98-99 -1.3313181315173672 1104 100 -1.3298759231408255 1105 1 -0.6655977366801125 1105 2 -0.6818489572067072 1105 3 -0.6887646717449947 1105 4 -0.5473383739313817 1105 5 -0.4329781446903809 1105 6 -0.4933204538849125 1105 7 -0.4703249728323158 1105 8 -0.5357573063277101 1105 9 -0.7637710519851026 1105 10-11 -0.7442916185070487 1105 12-13 -0.7756475982637383 1105 14-15 -0.9835029379731495 1105 16-17 -0.960448836021591 1105 18-19 -0.988761305919347 1105 20-21 -0.9837738987365441 1105 22-23 -1.01964250388545 1105 24-25 -1.0230528709667155 1105 26-27 -1.014391524733 1105 28-29 -1.0197680531909086 1105 30-31 -1.0773219700199874 1105 32-33 -1.1220974180536487 1105 34-35 -1.066616052235993 1105 36-37 -1.072607742866623 1105 38-39 -1.1358230075029851 1105 40-41 -1.2170955737030908 1105 42-43 -1.1645294863285045 1105 44-45 -1.2928581102414043 1105 46-47 -1.2928702109335788 1105 48-49 -1.308151423735815 1105 50-51 -1.236187686289668 1105 52-53 -1.1017420936829367 1105 54-55 -0.9933693031779924 1105 56-57 -0.9824559705057894 1105 58-59 -0.9645743263465576 1105 60-61 -0.9667471916975714 1105 62-63 -0.9987427470264194 1105 64-65 -0.9610569230493695 1105 66-67 -0.9749932163094073 1105 68-69 -0.9516881787197704 1105 70-71 -0.893357020395861 1105 72-73 -0.9705958653284128 1105 74-75 -0.9471889388161436 1105 76-77 -0.9456407427259101 1105 78-79 -1.03590351898427 1105 80-81 -1.0567877488232504 1105 82-83 -1.1603111014766192 1105 84-85 -1.093957446923259 1105 86-87 -1.1275697795844444 1105 88-89 -1.1111112051086813 1105 90-91 -1.185002698245274 1105 92-93 -1.159763190195271 1105 94-95 -1.238371005822593 1105 96-97 -1.2108748436319807 1105 98-99 -1.236053197814332 1105 100 -1.2654632756273934 1106 1 -0.5584281644153748 1106 2 -0.5415697683382383 1106 3 -0.5966753804481719 1106 4 -0.4111972468966627 1106 5 -0.4366744673778058 1106 6 -0.380441620559715 1106 7 -0.42615770934368413 1106 8 -0.45674764658181743 1106 9 -0.5684903464346789 1106 10-11 -0.5566986498829039 1106 12-13 -0.689190306052474 1106 14-15 -0.8385819966781796 1106 16-17 -0.8196176850987698 1106 18-19 -0.9014395768488583 1106 20-21 -0.8314571338884278 1106 22-23 -0.8281559260048681 1106 24-25 -0.8095811019598855 1106 26-27 -0.9049720693883785 1106 28-29 -0.8693710922736955 1106 30-31 -0.9485133424874519 1106 32-33 -0.9001456060903763 1106 34-35 -0.9387671976993559 1106 36-37 -0.9266494533890821 1106 38-39 -1.0318030559866926 1106 40-41 -1.0575699266702046 1106 42-43 -0.9449209129779206 1106 44-45 -1.0137080523967157 1106 46-47 -0.988674486812279 1106 48-49 -1.0883586910743688 1106 50-51 -1.0707620506503233 1106 52-53 -0.9630694416989556 1106 54-55 -0.8699004573582201 1106 56-57 -0.858656750749482 1106 58-59 -0.8785327398559559 1106 60-61 -0.8206878453624356 1106 62-63 -0.7943072942983278 1106 64-65 -0.8504169459071989 1106 66-67 -0.8269328336486339 1106 68-69 -0.8865859876670754 1106 70-71 -0.8807524574088248 1106 72-73 -0.840142525923433 1106 74-75 -0.8056982560188999 1106 76-77 -0.7744223013915068 1106 78-79 -0.8095024508657929 1106 80-81 -0.8112020818656767 1106 82-83 -0.8233920890502979 1106 84-85 -0.8190618182983869 1106 86-87 -0.8644851827056819 1106 88-89 -0.8330002273234278 1106 90-91 -0.9175064121999412 1106 92-93 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54-55 1.1244973399001807 2311 56-57 1.111206054293369 2311 58-59 1.0826662680829315 2311 60-61 1.0689319229335155 2311 62-63 1.106269470494098 2311 64-65 1.075529152261261 2311 66-67 1.096936867844498 2311 68-69 1.0788053997988314 2311 70-71 1.021924184004412 2311 72-73 1.0297790598465753 2311 74-75 1.080473453298687 2311 76-77 1.121339778547508 2311 78-79 1.1381477534359448 2311 80-81 1.0818950076460894 2311 82-83 1.1323148381620634 2311 84-85 1.2005826694064972 2311 86-87 1.2095170292757693 2311 88-89 1.2439390972776998 2311 90-91 1.2686122366501955 2311 92-93 1.2959325249611489 2311 94-95 1.291901169443051 2311 96-97 1.3708250605165055 2311 98-99 1.466320674025031 2311 100 1.5421626141018407 2312 1 0.6272347662200701 2312 2 0.6149724516806749 2312 3 0.6346186506894789 2312 4 0.3704229604482947 2312 5 0.4606798250323152 2312 6 0.5122227064676466 2312 7 0.41239020032362106 2312 8 0.5522967014690252 2312 9 0.7295574386813328 2312 10-11 0.668906410085917 2312 12-13 0.8141094356727123 2312 14-15 1.0494501709120456 2312 16-17 1.0188802524981284 2312 18-19 0.9947768415244767 2312 20-21 0.9871037999258974 2312 22-23 1.1020174238896203 2312 24-25 1.07746242691654 2312 26-27 1.111157722285597 2312 28-29 1.1382407401754406 2312 30-31 1.1519514691507524 2312 32-33 1.199349412062837 2312 34-35 1.2191430182713603 2312 36-37 1.2474237713508316 2312 38-39 1.24083669697945 2312 40-41 1.2863912566684164 2312 42-43 1.3568566716488704 2312 44-45 1.359529658800902 2312 46-47 1.346562794239702 2312 48-49 1.3393282783416538 2312 50-51 1.3655052158695895 2312 52-53 1.2518900078200517 2312 54-55 1.174976127478665 2312 56-57 1.1037298345050104 2312 58-59 1.074129366890098 2312 60-61 1.0745166193665838 2312 62-63 1.0783149671619299 2312 64-65 1.1175550898818116 2312 66-67 1.0778553817079057 2312 68-69 1.0775837486584479 2312 70-71 1.0249759327951224 2312 72-73 1.0386781977895794 2312 74-75 1.0631670692403787 2312 76-77 1.0613761120891496 2312 78-79 1.05869743428228 2312 80-81 1.0433925694161772 2312 82-83 1.0840746380655766 2312 84-85 1.1481077687588659 2312 86-87 1.1714568235114022 2312 88-89 1.1085402182861408 2312 90-91 1.1417701421624393 2312 92-93 1.2434801875876715 2312 94-95 1.2729625306806653 2312 96-97 1.2705090697328991 2312 98-99 0.9875434863246859 2312 100 1.1932282584779728 2313 1 0.6311705987240259 2313 2 0.6565827597661631 2313 3 0.6494613688908295 2313 4 0.513534370539027 2313 5 0.3611287680696549 2313 6 0.5225994129530918 2313 7 0.5982239594782115 2313 8 0.6129703351141274 2313 9 0.7758772551736186 2313 10-11 0.7737926725949364 2313 12-13 0.8611202078923483 2313 14-15 1.0129736895698969 2313 16-17 1.0581328805109678 2313 18-19 0.995074583831304 2313 20-21 1.0267245256975954 2313 22-23 1.0558371186950097 2313 24-25 1.0338982028634476 2313 26-27 1.0612261308642346 2313 28-29 1.1106760118429264 2313 30-31 1.1336939777229347 2313 32-33 1.1411976641245616 2313 34-35 1.2111642723005573 2313 36-37 1.2040655343448137 2313 38-39 1.1985702773435278 2313 40-41 1.1947361552802036 2313 42-43 1.2543049719195523 2313 44-45 1.1868776165026134 2313 46-47 1.2430777334413605 2313 48-49 1.248709218477984 2313 50-51 1.2615742379678991 2313 52-53 1.1776085395725104 2313 54-55 1.0457730007262498 2313 56-57 0.9731909957733862 2313 58-59 0.9768844332580002 2313 60-61 0.9245308121731526 2313 62-63 0.9510771672672007 2313 64-65 0.9738589301917031 2313 66-67 0.9647286922597971 2313 68-69 0.963584295819409 2313 70-71 0.9735438093278432 2313 72-73 1.0259520943673337 2313 74-75 1.0307418517959164 2313 76-77 0.7809786127383234 2313 78-79 0.9646816947675916 2313 80-81 1.058832805710317 2313 82-83 1.0898982121522671 2313 84-85 1.1211403595600338 2313 86-87 1.1090374173114093 2313 88-89 1.1671846650512485 2313 90-91 1.211321606289065 2313 92-93 1.2944505211109032 2313 94-95 1.2755082592960179 2313 96-97 1.3175248071526617 2313 98-99 1.3317906152832926 2313 100 1.4356386735987812 2314 1 0.5053062270162236 2314 2 0.48464133760520056 2314 3 0.5410797205643938 2314 4 0.4210356514018585 2314 5 0.4000729408367292 2314 6 0.4537611691441228 2314 7 0.47710261456838765 2314 8 0.46146586767606124 2314 9 0.6280613355590958 2314 10-11 0.5852487783068625 2314 12-13 0.645211930108708 2314 14-15 0.8026942615587274 2314 16-17 0.7958117964439495 2314 18-19 0.7972551819635498 2314 20-21 0.7994479337581808 2314 22-23 0.8552203163221037 2314 24-25 0.8275012311960808 2314 26-27 0.8888109140078768 2314 28-29 0.8367710711774023 2314 30-31 0.9323209315457248 2314 32-33 0.9486821630926343 2314 34-35 0.9426651169986613 2314 36-37 0.936192341818618 2314 38-39 0.9992464160918288 2314 40-41 1.0595089694092152 2314 42-43 1.1179337280802173 2314 44-45 1.1422938244635858 2314 46-47 1.162311282314647 2314 48-49 1.104984025936652 2314 50-51 1.1627201449304394 2314 52-53 1.0541982474264842 2314 54-55 0.9746657637457119 2314 56-57 0.9360317870062147 2314 58-59 0.9032707448467505 2314 60-61 0.9288492807935924 2314 62-63 0.901450614619776 2314 64-65 0.9257539893121773 2314 66-67 0.9602714972981374 2314 68-69 0.9411855921956018 2314 70-71 0.9676852361210635 2314 72-73 0.9834019111748091 2314 74-75 0.959445225227725 2314 76-77 1.0078764255024453 2314 78-79 1.0051627878699492 2314 80-81 0.9990532268743735 2314 82-83 0.9471493305434997 2314 84-85 1.0459289882848388 2314 86-87 1.0709960530675708 2314 88-89 1.075926529548692 2314 90-91 1.1423814146699804 2314 92-93 1.2215066932669671 2314 94-95 1.2262457841914198 2314 96-97 1.22020589993976 2314 98-99 1.333504470095967 2314 100 1.3519574131365601 2315 1 0.3961577001571541 2315 2 0.4540135119168447 2315 3 0.5190280683231414 2315 4 0.19170151508122046 2315 5 0.28888877142813385 2315 6 0.3611466795187326 2315 7 0.39852924123228206 2315 8 0.3932790368646266 2315 9 0.5208997604209742 2315 10-11 0.4005005167083908 2315 12-13 0.4737424019517249 2315 14-15 0.6882116552914255 2315 16-17 0.6958569098227869 2315 18-19 0.5420390626032656 2315 20-21 0.6403634468742183 2315 22-23 0.4485323712512965 2315 24-25 0.6160729471569013 2315 26-27 0.7248735659117713 2315 28-29 0.792409488976439 2315 30-31 0.8210287942664891 2315 32-33 0.7805465471162307 2315 34-35 0.9010767602639547 2315 36-37 0.9543084598884306 2315 38-39 0.9612055282576293 2315 40-41 0.9176282588554372 2315 42-43 0.99676425089757 2315 44-45 1.0114056970187875 2315 46-47 1.0941529594856831 2315 48-49 1.0850355234926141 2315 50-51 0.9893826621466317 2315 52-53 0.9846860859758166 2315 54-55 0.894437284837359 2315 56-57 0.8581003092991679 2315 58-59 0.8567375246501214 2315 60-61 0.8277575873102876 2315 62-63 0.8290940641841544 2315 64-65 0.8436142846294459 2315 66-67 0.8454623552490048 2315 68-69 0.9008570293840279 2315 70-71 0.8257291505767128 2315 72-73 0.6071478454575399 2315 74-75 0.804953578564259 2315 76-77 0.8470736120643281 2315 78-79 0.8343691520460368 2315 80-81 0.5304459352527786 2315 82-83 0.5743541058325157 2315 84-85 0.8717438662378534 2315 86-87 0.9667913684460352 2315 88-89 0.9537504472995231 2315 90-91 1.0334051065179217 2315 92-93 1.1290040924385636 2315 94-95 1.1403520557479254 2315 96-97 0.9693210521499154 2315 98-99 0.3672439636458762 2315 100 0.9068528530773534 2316 1 0.3147167670807818 2316 2 0.2741090526261125 2316 3 0.35277444194926844 2316 4 0.20883941674025408 2316 5 0.1182736737897443 2316 6 0.18113750876952395 2316 7 0.17828974316087454 2316 8 0.241442424403445 2316 9 0.2849411190513109 2316 10-11 0.2966627085954485 2316 12-13 0.06246386523780245 2316 14-15 0.11706360396420479 2316 16-17 0.2628511438395762 2316 18-19 0.37409512674724965 2316 20-21 0.3596050521007683 2316 22-23 0.3204428678590645 2316 24-25 0.3889600756259526 2316 26-27 0.42548614242986105 2316 28-29 0.40586653827951835 2316 30-31 0.29548141865522126 2316 32-33 0.5282334150602281 2316 34-35 0.470433186502099 2316 36-37 0.4539067465295048 2316 38-39 0.6591115553787574 2316 40-41 0.7469295519533574 2316 42-43 0.5920794020784257 2316 44-45 0.5286553581300666 2316 46-47 0.366833297588812 2316 48-49 0.3084806524931807 2316 50-51 0.4127521380377601 2316 52-53 0.5533227449036175 2316 54-55 0.21199416047564767 2316 56-57 0.12222339958536566 2316 58-59 0.19193556491784847 2316 60-61 0.2443453610189792 2316 62-63 0.3462727021328931 2316 64-65 0.31545679177632735 2316 66-67 0.30133103858904775 2316 68-69 0.27507120872176216 2316 70-71 0.06503044367463673 2316 72-73 -0.06096979976594952 2316 74-75 0.1698802627144076 2316 76-77 0.3200274791842901 2316 78-79 0.33080024021727183 2316 80-81 0.5355876333568403 2316 82-83 0.6107168591076011 2316 84-85 0.701205212867098 2316 86-87 0.7406549894879468 2316 88-89 0.709564861087518 2316 90-91 0.45757488906641797 2316 92-93 0.5665797107259039 2316 94-95 0.7254710888411786 2316 96-97 0.705795010509366 2316 98-99 0.5417072937421459 2316 100 0.5207901131182382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 44.0 1 679.0 2 247.0 3 247.0 4 210.0 5 162.0 6 187.0 7 708.0 8 2321.0 9 4606.0 10 7291.0 11 10615.0 12 15029.0 13 19013.0 14 23251.0 15 27549.0 16 33012.0 17 41432.0 18 50751.0 19 62848.0 20 79231.0 21 98879.0 22 108535.0 23 107110.0 24 104693.0 25 109359.0 26 121153.0 27 142045.0 28 167972.0 29 203382.0 30 249263.0 31 310968.0 32 400019.0 33 530653.0 34 774260.0 35 1080363.0 36 1654438.0 37 2255399.0 38 1824892.0 39 553572.0 40 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97178084171097 21.2146313816105 12.852354529893015 22.961233246785522 2 15.42585291890511 19.594423402415455 41.80788060120834 23.1718430774711 3 19.177342879246233 26.68491015247301 28.267449024655107 25.87029794362566 4 12.333096313170154 16.19800448471023 39.84770097302355 31.621198229096066 5 12.974549196832072 38.64504899548078 34.55959550762898 13.82080630005817 6 29.561694932611832 38.440438127819625 17.920366340685952 14.077500598882587 7 27.3052902845071 31.958810878664906 23.08643393319261 17.649464903635387 8 26.37151467827321 35.56680852884626 19.527093444366756 18.534583348513777 9 26.314544367427604 15.374663449145018 21.938085383667975 36.3727067997594 10-11 26.16527979058077 26.229152440546567 26.698293295224563 20.9072744736481 12-13 25.93526283097643 23.84512115814076 27.690047187580042 22.52956882330277 14-15 23.40360472196241 24.966634379377084 25.457619531877256 26.17214136678325 16-17 21.663835515366852 27.82996447581866 26.799787825848064 23.706412182966424 18-19 21.005725992726365 28.01594058959832 29.275781587291018 21.702551830384294 20-21 22.728956775993346 27.050941310422093 28.640614964478083 21.579486949106478 22-23 23.242993602912712 26.820272055981103 28.23538055847667 21.701353782629514 24-25 22.501159949326915 26.681461157146387 29.21330757766291 21.604071315863788 26-27 22.42622105514528 27.01365554297886 28.01812583624758 22.54199756562828 28-29 21.488028004784397 27.442539201633124 28.726493025023935 22.342939768558548 30-31 22.566202310177523 27.088650050105795 28.64102806519512 21.70411957452157 32-33 22.81978558095819 27.29241760948654 27.705451355461346 22.182345454093923 34-35 22.809276193945067 27.134751093926678 28.546848341200416 21.509124370927836 36-37 22.3104365121785 26.682391014247713 28.199419760477245 22.807752713096537 38-39 22.637993396067486 26.940286068986687 28.665760876252666 21.75595965869316 40-41 22.31616311400356 25.845162629261697 29.30735072798759 22.531323528747144 42-43 22.259127976261066 26.087613178134657 28.618950426489086 23.034308419115195 44-45 22.37179970008422 27.072359883895082 28.449876171849493 22.105964244171204 46-47 22.38996468176462 26.862335237551843 27.74496215769081 23.002737922992722 48-49 23.11627472136639 26.817048876530485 27.722001404641784 22.344674997461343 50-51 22.70384056342571 26.487779683750524 27.876238617525683 22.93214113529808 52-53 22.190138403480717 27.141183168351617 27.553497608392757 23.11518081977491 54-55 22.04681172536559 27.076469193811192 27.567910368977383 23.30880871184583 56-57 23.09689940936869 26.609642180606368 27.108905905346468 23.18455250467847 58-59 22.068605591961017 27.099831818500398 27.995345268858117 22.836217320680472 60-61 22.165487205357284 27.797091667398266 27.274932032977127 22.762489094267323 62-63 22.605081048427696 26.92015079817879 26.881695025226254 23.593073128167266 64-65 21.84676104155497 27.06609401315399 27.356354026021425 23.730790919269616 66-67 22.408269783338348 26.720192862206332 27.241101967720226 23.630435386735094 68-69 21.90921284529726 26.79426222754865 27.56773632343212 23.728788603721974 70-71 21.987794143630722 26.43368195530003 27.624416653583573 23.954107247485673 72-73 22.699787602572936 26.538377552549868 27.795705693438865 22.96612915143833 74-75 22.950217267295244 26.84823972949564 27.302181336079762 22.899361667129355 76-77 22.593284562969497 26.8036245990025 26.94173843922456 23.661352398803434 78-79 22.52866140750015 26.87477163450675 26.892588953029843 23.703978004963254 80-81 22.068828839922727 27.368282950897193 27.532059502780815 23.030828706399266 82-83 23.102977931632026 26.334580846160378 28.63232998055126 21.930111241656338 84-85 23.202107924842807 26.77750792793891 27.288784933913934 22.731599213304353 86-87 21.88329256044759 27.54388780535537 27.72089270315754 22.851926931039497 88-89 23.080903582379534 26.25115848111085 27.538580950183377 23.129356986326243 90-91 22.758199159372648 26.482741679245652 27.55152908759917 23.207530073782536 92-93 23.21696296530626 26.6148976485185 27.43592704770863 22.73221233846661 94-95 22.009840960083363 26.63054329234007 28.1819594987092 23.177656248867358 96-97 22.455797087895533 26.53136335205868 27.628953369439234 23.383886190606553 98-99 21.799237956203335 27.036423058707122 27.46113709091651 23.703201894173034 100 22.779623221590008 26.446328551289017 26.53895873294083 24.23508949418014 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 843.0 1 629.0 2 597.0 3 823.0 4 742.5 5 672.0 6 908.0 7 1676.5 8 2759.5 9 3456.0 10 3454.0 11 3540.5 12 4288.0 13 5251.5 14 6302.0 15 7642.0 16 8938.5 17 10124.5 18 11274.0 19 12128.0 20 13055.5 21 14167.0 22 15767.0 23 17837.5 24 20068.0 25 22939.0 26 27658.0 27 33699.5 28 42473.5 29 60996.0 30 89188.0 31 116665.0 32 154409.0 33 198414.5 34 233992.0 35 270121.5 36 305155.0 37 357739.5 38 381438.5 39 364785.0 40 338719.0 41 306175.5 42 296785.0 43 312064.0 44 342190.0 45 401504.0 46 460272.5 47 500458.5 48 527509.5 49 519902.0 50 495928.0 51 511353.0 52 515379.5 53 482615.0 54 448465.5 55 379822.5 56 326629.0 57 265014.5 58 202537.5 59 193665.0 60 160044.0 61 106210.0 62 76645.0 63 65661.5 64 49111.5 65 22614.0 66 11820.0 67 7809.0 68 5672.5 69 4333.0 70 3328.5 71 2463.5 72 1838.5 73 1447.5 74 1131.5 75 843.5 76 628.5 77 451.5 78 324.0 79 248.5 80 190.5 81 133.5 82 115.0 83 103.0 84 75.0 85 51.5 86 41.0 87 41.0 88 35.0 89 26.5 90 24.0 91 17.5 92 14.5 93 13.5 94 10.5 95 8.0 96 6.5 97 4.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0573513055563931 2 0.13367954076724953 3 0.017572225290601567 4 0.01674013926614844 5 0.019943223102215326 6 0.05301193220306228 7 0.05813865577307994 8 0.04972832391301605 9 0.04087958113684245 10-11 0.04024433266656103 12-13 0.04129562414906902 14-15 0.018368522668841664 16-17 0.01741564996341953 18-19 0.017764589263996648 20-21 0.021115301265692315 22-23 0.025190733353202 24-25 0.024600220690686875 26-27 0.01952718008998876 28-29 0.018493782930587295 30-31 0.01676250717003159 32-33 0.023097097549739286 34-35 0.022672107375959462 36-37 0.02281973554158824 38-39 0.020815571353658125 40-41 0.02451969623670754 42-43 0.031529797313686325 44-45 0.04108983943334405 46-47 0.047460218459264784 48-49 0.04013249314714529 50-51 0.027575151907145642 52-53 0.03964039926171602 54-55 0.036499945556521945 56-57 0.03826700996329069 58-59 0.04357267676437355 60-61 0.022904733576344205 62-63 0.025535199073002484 64-65 0.024891003441167805 66-67 0.03194584032591288 68-69 0.03887094336813571 70-71 0.04071853222888378 72-73 0.04458817960066849 74-75 0.0262599191588165 76-77 0.020144534237163666 78-79 0.019755332709596878 80-81 0.023826291216329933 82-83 0.019934275940662067 84-85 0.01232918862039153 86-87 0.014445192327737392 88-89 0.01856983380379 90-91 0.02090504296919072 92-93 0.01986717222901262 94-95 0.023405774623326735 96-97 0.024586799948356983 98-99 0.027673570684231496 100 0.029212482471392125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1176729E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.150437648616965 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88397778438683 45.18037576735948 2 15.213468045263397 18.60620458242212 3 5.339787248051377 9.795909815065372 4 2.358579373242098 5.7691264361101995 5 1.1831678787890074 3.6175616799866797 6 0.6631179971089529 2.4329973441532076 7 0.38621419616203534 1.6532016984992088 8 0.24171267841434793 1.182466885620543 9 0.16363420847442112 0.9005673116246289 >10 0.5219248040326723 5.017535814012191 >50 0.022539447976328005 0.9677650131156105 >100 0.01906327473997779 2.3120633232216257 >500 0.0019632705451846128 0.8339071041808744 >1k 7.486270271947754E-4 0.6999217487762971 >5k 4.0466314522278415E-5 0.14363425887564463 >10k+ 6.069947178341763E-5 0.8867612169761674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31605 0.28277504089076505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24877 0.22257853796043545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15189 0.13589843683245786 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.4735807766297275E-5 0.0 0.0 0.0 6 0.0 4.4735807766297275E-5 0.0 0.0 0.0 7 0.0 4.4735807766297275E-5 0.0 0.0 0.0 8 0.0 4.4735807766297275E-5 0.0 0.0 0.0 9 0.0 4.4735807766297275E-5 0.0 0.0 0.0 10-11 0.0 4.4735807766297275E-5 0.0 4.473580776629728E-6 0.0 12-13 0.0 4.920938854292701E-5 0.0 8.947161553259456E-6 0.0 14-15 0.0 5.3682969319556735E-5 0.0 1.789432310651891E-5 0.0 16-17 0.0 5.3682969319556735E-5 0.0 4.920938854292701E-5 0.0 18-19 0.0 6.26301308728162E-5 0.0 9.841877708585402E-5 0.0 20-21 0.0 7.157729242607565E-5 0.0 1.6999606951192964E-4 0.0 22-23 0.0 7.157729242607565E-5 0.0 3.355185582472296E-4 0.0 24-25 0.0 7.157729242607565E-5 0.0 7.605087320270537E-4 0.0 26-27 0.0 7.157729242607565E-5 0.0 0.001659698468129629 0.0 28-29 0.0 7.157729242607565E-5 0.0 0.004826993657983477 0.0 30-31 0.0 8.05244539793351E-5 0.0 0.01869956764631226 0.0 32-33 0.0 8.05244539793351E-5 0.0 0.05211721604773633 0.0 34-35 0.0 9.394519630922428E-5 0.0 0.10025741878504882 0.0 36-37 0.0 1.297338425222621E-4 0.0 0.18234762603620433 0.0 38-39 0.0 1.5657532718204046E-4 0.0 0.30135829543688497 0.0 40-41 0.0 1.610489079586702E-4 0.0 0.4229189058802446 0.0 42-43 0.0 1.6552248873529993E-4 0.0 0.5431508628329451 0.0 44-45 0.0 1.744696502885594E-4 0.0 0.6817468688737107 4.473580776629728E-6 46-47 0.0 1.8789039261844857E-4 0.0 0.8477748722367698 8.947161553259456E-6 48-49 0.0 1.96837554171708E-4 0.0 1.0331376917164226 8.947161553259456E-6 50-51 0.0 2.0578471572496747E-4 0.0 1.2243430076903539 8.947161553259456E-6 52-53 0.0 2.1920545805485667E-4 0.0 1.4053575066551223 8.947161553259456E-6 54-55 0.0 2.95256331257562E-4 0.0 1.5986698791748462 8.947161553259456E-6 56-57 0.0 3.220978159173404E-4 0.0 1.8239236184397063 8.947161553259456E-6 58-59 0.0 4.0709585067330524E-4 0.0 2.0849749510791575 8.947161553259456E-6 60-61 0.0 4.4735807766297275E-4 0.0 2.3489698998696307 8.947161553259456E-6 62-63 0.0 4.563052392162322E-4 0.0 2.6160560929767556 1.789432310651891E-5 64-65 0.0 4.831467238760106E-4 0.0 2.884448571670656 1.789432310651891E-5 66-67 0.0 5.457768547488268E-4 0.0 3.1817716972470214 1.789432310651891E-5 68-69 0.0 5.591975970787159E-4 0.0 3.5083654618448743 1.789432310651891E-5 70-71 0.0 5.860390817384942E-4 0.0 3.8651380023618715 1.789432310651891E-5 72-73 0.0 6.084069856216429E-4 0.0 4.222595000737694 1.789432310651891E-5 74-75 0.0 6.084069856216429E-4 0.0 4.562573719019223 1.789432310651891E-5 76-77 0.0 6.173541471749024E-4 0.0 4.945145399875043 1.789432310651891E-5 78-79 0.0 6.307748895047916E-4 0.0 5.355753011458004 1.789432310651891E-5 80-81 0.0 6.620899549411997E-4 0.0 5.791868980629306 1.789432310651891E-5 82-83 0.0 6.620899549411997E-4 0.0 6.2337737633255665 1.789432310651891E-5 84-85 0.0 6.710371164944591E-4 0.0 6.6505280748956155 1.789432310651891E-5 86-87 0.0 6.799842780477187E-4 0.0 7.092759428988571 1.789432310651891E-5 88 0.0 6.799842780477187E-4 0.0 7.447823061648895 1.789432310651891E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21180 0.0 44.013596 1 GTATCAA 36675 0.0 39.08302 1 GGGCACC 12505 0.0 38.108727 94 GTACTAG 12430 0.0 37.290092 1 GCATCGT 11770 0.0 36.90178 8 CAACGCA 39915 0.0 33.599964 5 ATCAACG 40705 0.0 33.08674 3 TAGGCAT 15310 0.0 32.945625 5 TACCTGG 5240 0.0 32.86778 2 CTGTGCG 16795 0.0 31.68241 9 TATCAAC 45280 0.0 31.364138 2 TCTAACG 6365 0.0 30.607122 2 CTAACGC 6415 0.0 30.337566 3 TAGTACT 17935 0.0 30.063068 4 CTAGGCA 17235 0.0 30.002169 4 ACTGTGC 18400 0.0 29.915127 8 ACCTGGG 5705 0.0 29.581205 3 GTGGTAT 7110 0.0 29.452269 1 AACGCAG 47055 0.0 29.42147 6 TAACGCC 6625 0.0 29.375528 4 >>END_MODULE