FastQCFastQC Report
Mon 6 Jun 2016
ERR1211207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11176729
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297010.2657396452933591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248500.22233696459849744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150400.1345653097610222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214200.043.815741
GTATCAA372250.038.538661
GGGCACC125600.038.10185694
GTACTAG126850.037.623971
TAGGCAT154550.033.9284135
CAACGCA414600.032.547945
CTGTGCG163600.032.539959
ATCAACG422300.031.9990223
TAGTACT176250.030.8282344
ACTGTGC180200.030.7938148
CTAGGCA176000.030.3379064
TATCAAC475100.030.1218322
TACCTGG49400.029.6829282
GCATCGT154450.029.3567358
TACCCCG6450.029.1385945
GTACTGT189600.028.870616
AACGCAG488800.028.587636
TACTGTG206200.028.4376897
GTGGTAT67450.028.3030471
AAGCTAA71850.027.4658379
Produced by FastQC (version 0.11.5)