##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211207_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11176729 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16690464625205 33.0 31.0 34.0 30.0 34.0 2 32.31303049398442 34.0 31.0 34.0 31.0 34.0 3 32.392942156868976 34.0 31.0 34.0 31.0 34.0 4 35.99905034827274 37.0 35.0 37.0 35.0 37.0 5 35.890342693287096 37.0 35.0 37.0 35.0 37.0 6 35.91590330229891 37.0 35.0 37.0 35.0 37.0 7 35.85833216498315 37.0 35.0 37.0 35.0 37.0 8 35.850192126873615 37.0 35.0 37.0 35.0 37.0 9 37.45362010656248 39.0 37.0 39.0 35.0 39.0 10-11 37.43427361440006 39.0 37.0 39.0 34.5 39.0 12-13 37.32291849431081 39.0 37.0 39.0 34.0 39.0 14-15 38.53364727730269 40.0 38.0 41.0 34.0 41.0 16-17 38.492394152171 40.0 38.0 41.0 34.0 41.0 18-19 38.52159339284329 40.0 38.0 41.0 34.0 41.0 20-21 38.48386522568454 40.0 38.0 41.0 34.0 41.0 22-23 38.342550177247745 40.0 38.0 41.0 34.0 41.0 24-25 38.270653918512295 40.0 38.0 41.0 34.0 41.0 26-27 38.12805598131618 40.0 37.5 41.0 33.0 41.0 28-29 38.11770885739468 40.0 37.5 41.0 33.5 41.0 30-31 38.13577147661002 40.0 37.5 41.0 33.5 41.0 32-33 38.015861214850965 40.0 37.0 41.0 33.0 41.0 34-35 37.88034866909629 40.0 37.0 41.0 33.0 41.0 36-37 37.74654632853673 40.0 37.0 41.0 33.0 41.0 38-39 37.649856187798775 40.0 36.5 41.0 33.0 41.0 40-41 37.543348103009386 40.0 36.0 41.0 32.5 41.0 42-43 37.393996803537064 40.0 36.0 41.0 32.0 41.0 44-45 37.21954826854977 39.0 36.0 41.0 31.5 41.0 46-47 36.95801208922575 39.0 35.0 41.0 31.0 41.0 48-49 36.685731666214686 39.0 35.0 41.0 30.5 41.0 50-51 36.45991644782655 39.0 35.0 40.5 30.0 41.0 52-53 36.23030253305775 38.5 35.0 40.0 30.0 41.0 54-55 35.971333204911744 38.0 35.0 40.0 29.0 41.0 56-57 35.67586549696249 38.0 34.5 40.0 29.0 41.0 58-59 35.36280391159166 37.0 34.0 40.0 28.0 41.0 60-61 35.09347770711807 37.0 34.0 40.0 28.0 41.0 62-63 34.77460865339045 36.0 34.0 39.0 27.5 41.0 64-65 34.397099231805655 35.5 33.5 39.0 27.0 40.5 66-67 34.10518412855854 35.0 33.0 39.0 26.0 40.0 68-69 33.79778381492474 35.0 33.0 38.0 26.0 40.0 70-71 33.53287477937418 35.0 33.0 37.5 26.0 40.0 72-73 33.236200054595585 35.0 32.5 37.0 26.0 39.5 74-75 32.928960163568426 35.0 32.0 36.5 25.5 39.0 76-77 31.967218673728244 34.0 30.5 35.0 24.5 38.0 78-79 32.48476204442284 35.0 32.0 36.0 25.0 38.0 80-81 32.43799022057348 35.0 32.0 35.0 25.0 37.0 82-83 32.213024400967406 35.0 32.0 35.0 25.0 37.0 84-85 31.98665960318086 34.0 32.0 35.0 25.0 36.5 86-87 31.77391976668666 34.0 31.0 35.0 24.0 36.0 88-89 31.57629477282665 34.0 31.0 35.0 24.0 36.0 90-91 31.372284234501883 34.0 31.0 35.0 24.0 35.0 92-93 31.18715153601738 34.0 31.0 35.0 23.0 35.0 94-95 31.010651640564962 34.0 31.0 35.0 23.0 35.0 96-97 30.853519039425578 34.0 30.0 35.0 21.5 35.0 98-99 30.68799972693263 34.0 30.0 35.0 20.0 35.0 100 28.8204144522069 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32362513914106117 1101 2 -0.31674929746285585 1101 3 -0.38601346035729023 1101 4 -0.19589137697565207 1101 5 -0.2516576871076168 1101 6 -0.3093812722214082 1101 7 -0.2978091935360183 1101 8 -0.3272060026532202 1101 9 -0.5397420353154487 1101 10-11 -0.4543529883774795 1101 12-13 -0.45668767537416244 1101 14-15 -0.7117741195176208 1101 16-17 -0.6392049819085202 1101 18-19 -0.7311385226566856 1101 20-21 -0.7409474447248598 1101 22-23 -0.7275055418188217 1101 24-25 -0.6896983980881544 1101 26-27 -0.8286163024543569 1101 28-29 -0.8699315027121983 1101 30-31 -0.697637285227195 1101 32-33 -0.7121300027260489 1101 34-35 -0.7615829439757249 1101 36-37 -0.6766017857700675 1101 38-39 -0.7692701036035743 1101 40-41 -0.8012603554533939 1101 42-43 -0.7913819314817161 1101 44-45 -0.8192856793013732 1101 46-47 -0.8616203800909972 1101 48-49 -0.7819109935139252 1101 50-51 -0.9016260126225859 1101 52-53 -0.8641328483004855 1101 54-55 -0.8288762740452071 1101 56-57 -0.8064117914869513 1101 58-59 -0.8218997560389099 1101 60-61 -0.9024978864273905 1101 62-63 -0.9101851561252516 1101 64-65 -0.8628063218551176 1101 66-67 -0.9314487937858544 1101 68-69 -1.0742758174523246 1101 70-71 -1.0236176484460984 1101 72-73 -1.012841269668563 1101 74-75 -1.1207881894407095 1101 76-77 -1.0447203833112972 1101 78-79 -0.8816720965073443 1101 80-81 -0.8416069460375084 1101 82-83 -0.8668656933177203 1101 84-85 -0.8101283743488068 1101 86-87 -1.0390181596485384 1101 88-89 -0.9795467578773867 1101 90-91 -1.0207457617272233 1101 92-93 -1.0865463540055735 1101 94-95 -1.0874064972204245 1101 96-97 -1.1257950224241888 1101 98-99 -1.297865896198875 1101 100 -1.1725879567218946 1102 1 -0.4358006215721524 1102 2 -0.338369321305624 1102 3 -0.3788548551269635 1102 4 -0.282859290109748 1102 5 -0.3093052695708707 1102 6 -0.33283978631534694 1102 7 -0.31521820644484677 1102 8 -0.3363114814518937 1102 9 -0.49740094081085573 1102 10-11 -0.5128502477727395 1102 12-13 -0.5069033880782214 1102 14-15 -0.6556981928751995 1102 16-17 -0.6604450826961141 1102 18-19 -0.6691545569966166 1102 20-21 -0.7019040966065688 1102 22-23 -0.7195676878024386 1102 24-25 -0.7854520831771268 1102 26-27 -0.8418276581217015 1102 28-29 -0.846629240989536 1102 30-31 -0.8058266733619845 1102 32-33 -0.7299319356977065 1102 34-35 -0.7854831853164796 1102 36-37 -0.8256485720366342 1102 38-39 -0.8070107411236123 1102 40-41 -0.7603963268932787 1102 42-43 -0.7945065098424209 1102 44-45 -0.7959736453925075 1102 46-47 -0.8692298984231641 1102 48-49 -0.855364217434861 1102 50-51 -0.8623202910769479 1102 52-53 -0.9049420011489815 1102 54-55 -0.8712698305314248 1102 56-57 -0.9320806744235597 1102 58-59 -0.9582056441123754 1102 60-61 -0.9392147017711991 1102 62-63 -0.9875130567615074 1102 64-65 -0.9175823327445372 1102 66-67 -0.9583653572085566 1102 68-69 -1.061569024189069 1102 70-71 -1.0197990249908386 1102 72-73 -0.983893195772815 1102 74-75 -1.1289452189353284 1102 76-77 -1.036049058315129 1102 78-79 -0.904159587547106 1102 80-81 -0.8566936526423845 1102 82-83 -0.7951806047933943 1102 84-85 -0.8632434653825314 1102 86-87 -0.8458641076901934 1102 88-89 -0.9643757857108888 1102 90-91 -0.9796696816126165 1102 92-93 -1.0202632322920273 1102 94-95 -1.1539056917430912 1102 96-97 -1.2094507165642199 1102 98-99 -1.2407524076864185 1102 100 -1.1870313065620444 1103 1 -0.34089084384111246 1103 2 -0.33938200158562637 1103 3 -0.35382754660158255 1103 4 -0.25755859316104335 1103 5 -0.253549354199599 1103 6 -0.3401575658384388 1103 7 -0.38801291758119305 1103 8 -0.3785878678976786 1103 9 -0.502351581942051 1103 10-11 -0.5041661230714851 1103 12-13 -0.5789881889519037 1103 14-15 -0.7240436253994744 1103 16-17 -0.6957865904015961 1103 18-19 -0.634314125091791 1103 20-21 -0.6402485677308718 1103 22-23 -0.7002012690927373 1103 24-25 -0.8221730969091468 1103 26-27 -0.747163010592395 1103 28-29 -0.8173267483746756 1103 30-31 -0.7609278327659794 1103 32-33 -0.685960192264794 1103 34-35 -0.8130196424881007 1103 36-37 -0.8107736923632203 1103 38-39 -0.8116699493682873 1103 40-41 -0.8385428312201242 1103 42-43 -0.767490761637049 1103 44-45 -0.8070799020306865 1103 46-47 -0.8483919182527799 1103 48-49 -0.9563569580895077 1103 50-51 -0.9363016689089179 1103 52-53 -0.9343422412649005 1103 54-55 -0.9200103086693687 1103 56-57 -0.8585948401356234 1103 58-59 -0.8383690879363925 1103 60-61 -0.9067848061558053 1103 62-63 -0.8686713150138488 1103 64-65 -0.8672387551666318 1103 66-67 -0.9177737050651231 1103 68-69 -0.9254957836541919 1103 70-71 -0.9157854842075679 1103 72-73 -0.9252180491203106 1103 74-75 -0.9715594650404284 1103 76-77 -0.8413767267304841 1103 78-79 -0.7802602304724076 1103 80-81 -0.7218972071130345 1103 82-83 -0.8153673156812609 1103 84-85 -0.8638380584115453 1103 86-87 -0.8855044796116331 1103 88-89 -0.9029100832718981 1103 90-91 -0.8935175648231741 1103 92-93 -1.0426394900237774 1103 94-95 -1.016366473707496 1103 96-97 -1.0003539118405342 1103 98-99 -1.1497642995563986 1103 100 -1.2879571619050871 1104 1 -0.38470198161117963 1104 2 -0.33482452983932376 1104 3 -0.4537066008803059 1104 4 -0.31757136776602835 1104 5 -0.3418843709271613 1104 6 -0.39556180365302396 1104 7 -0.3732909988705728 1104 8 -0.4031237037519375 1104 9 -0.5383424252787492 1104 10-11 -0.5159681976306203 1104 12-13 -0.5697311060759276 1104 14-15 -0.7088388416394906 1104 16-17 -0.6671471108645335 1104 18-19 -0.7171755031053735 1104 20-21 -0.7668012225082279 1104 22-23 -0.7005875110684556 1104 24-25 -0.7412620364458817 1104 26-27 -0.7877757827776364 1104 28-29 -0.8831643590312481 1104 30-31 -0.7904251132603477 1104 32-33 -0.7736559781053387 1104 34-35 -0.7987039078210714 1104 36-37 -0.7913663055375793 1104 38-39 -0.7659652747056072 1104 40-41 -0.7993587121150796 1104 42-43 -0.696710063055562 1104 44-45 -0.8264922952331943 1104 46-47 -0.9494998294546733 1104 48-49 -0.9324764152480682 1104 50-51 -0.85107369310807 1104 52-53 -0.8416560989578059 1104 54-55 -0.9347385270708912 1104 56-57 -0.8892313216873333 1104 58-59 -0.9605107492107692 1104 60-61 -0.895679368938481 1104 62-63 -0.9742054057285614 1104 64-65 -0.9119484541457084 1104 66-67 -1.0096834000391013 1104 68-69 -1.006859837784809 1104 70-71 -1.1059213781357968 1104 72-73 -1.060766745939553 1104 74-75 -1.0535345057485301 1104 76-77 -1.01844735377502 1104 78-79 -0.8691895458545424 1104 80-81 -0.8568009574316662 1104 82-83 -0.8051156743197438 1104 84-85 -0.8316577353917651 1104 86-87 -0.9076826039975678 1104 88-89 -0.9349855644160066 1104 90-91 -0.9540175016176633 1104 92-93 -0.9790412734621015 1104 94-95 -1.0218219225855059 1104 96-97 -1.1215598395576336 1104 98-99 -1.1504535942121343 1104 100 -1.2244548591803266 1105 1 -0.4652509022745335 1105 2 -0.4107576531215642 1105 3 -0.4839434639575657 1105 4 -0.2845649719639809 1105 5 -0.33661741328945993 1105 6 -0.39791584000315083 1105 7 -0.4772989699153882 1105 8 -0.43417916508499843 1105 9 -0.5957846773769475 1105 10-11 -0.5967808429722012 1105 12-13 -0.6673407100484496 1105 14-15 -0.8326565319331181 1105 16-17 -0.8020813184777253 1105 18-19 -0.7811048777086214 1105 20-21 -0.9102618446687387 1105 22-23 -0.8860135057850655 1105 24-25 -0.8676799913113911 1105 26-27 -0.9538926778296926 1105 28-29 -0.8935702571625512 1105 30-31 -0.8685264022970358 1105 32-33 -0.8210053257983105 1105 34-35 -0.8328483034832601 1105 36-37 -0.88909163513609 1105 38-39 -0.9220919879939089 1105 40-41 -0.9429198382652828 1105 42-43 -0.9378265906726782 1105 44-45 -0.9299319824950345 1105 46-47 -0.8529998837039869 1105 48-49 -0.9263952311283319 1105 50-51 -0.9394549600855555 1105 52-53 -1.0286378132417013 1105 54-55 -0.986818644178264 1105 56-57 -0.9573007847726629 1105 58-59 -1.0542455714560433 1105 60-61 -0.9916353892688221 1105 62-63 -0.9891163652411592 1105 64-65 -0.9611218191280599 1105 66-67 -1.0007411521067269 1105 68-69 -0.9855217436179231 1105 70-71 -1.0666186264365294 1105 72-73 -0.9801580246534201 1105 74-75 -1.0465759197393112 1105 76-77 -1.0117729377414868 1105 78-79 -0.9873412956452938 1105 80-81 -0.8774076828673323 1105 82-83 -0.8619667399700219 1105 84-85 -0.883469283296435 1105 86-87 -0.9140573475771241 1105 88-89 -0.9869269315342564 1105 90-91 -0.9155429997775393 1105 92-93 -1.0299741276040955 1105 94-95 -0.9338114793821966 1105 96-97 -1.006264280970857 1105 98-99 -1.1215997047891797 1105 100 -1.1611224516894474 1106 1 -0.44668774323089977 1106 2 -0.413356352784195 1106 3 -0.4457833627746872 1106 4 -0.29533236365571724 1106 5 -0.36040722932286684 1106 6 -0.37307267435038227 1106 7 -0.3895552708887635 1106 8 -0.4205249741714425 1106 9 -0.5636081554956789 1106 10-11 -0.5539540337541382 1106 12-13 -0.6270136532088415 1106 14-15 -0.7853207444442845 1106 16-17 -0.7211538538290228 1106 18-19 -0.7335384926574164 1106 20-21 -0.7685276673537373 1106 22-23 -0.8098116698334579 1106 24-25 -0.753594537264128 1106 26-27 -0.7664536804286897 1106 28-29 -0.7843432704529008 1106 30-31 -0.859138966407798 1106 32-33 -0.9013673418621693 1106 34-35 -0.8838679588854532 1106 36-37 -0.8521974806700641 1106 38-39 -0.8415859589989054 1106 40-41 -0.8439472852709571 1106 42-43 -0.739101992700256 1106 44-45 -0.8490617987524871 1106 46-47 -0.8793898733377787 1106 48-49 -0.8870480573229216 1106 50-51 -0.9799579866693477 1106 52-53 -0.9183464131673844 1106 54-55 -0.9733215658176917 1106 56-57 -0.9848939866474495 1106 58-59 -0.9095722793408854 1106 60-61 -0.9369597510132408 1106 62-63 -0.9282165549811694 1106 64-65 -0.9158129263604238 1106 66-67 -0.9638435615232979 1106 68-69 -0.9882225595309109 1106 70-71 -1.0281419786957215 1106 72-73 -1.0584341700615099 1106 74-75 -1.0945781297615866 1106 76-77 -1.0706196908850636 1106 78-79 -0.8174154056042937 1106 80-81 -0.7944852987346849 1106 82-83 -0.8033383046448641 1106 84-85 -0.8512495750388354 1106 86-87 -0.8348943706679783 1106 88-89 -0.937373420445013 1106 90-91 -0.903965909590088 1106 92-93 -0.8861949077092355 1106 94-95 -0.9107007525348472 1106 96-97 -0.8735633430895184 1106 98-99 -0.8696006674916248 1106 100 -0.9475272772437542 1107 1 -0.40801089828573467 1107 2 -0.4457292173634322 1107 3 -0.4766062627592049 1107 4 -0.2976639728321615 1107 5 -0.33224067728978923 1107 6 -0.33356463011789117 1107 7 -0.4283793840435379 1107 8 -0.4051136510798372 1107 9 -0.5509431529195936 1107 10-11 -0.5849496455295053 1107 12-13 -0.5897663758623892 1107 14-15 -0.7502171681300354 1107 16-17 -0.7096057990891467 1107 18-19 -0.714138999244831 1107 20-21 -0.7298742673044956 1107 22-23 -0.7442568538267125 1107 24-25 -0.7875358304833142 1107 26-27 -0.8432296233640031 1107 28-29 -0.8341202425525225 1107 30-31 -0.8561296442857156 1107 32-33 -0.8326534926861342 1107 34-35 -0.8152465568362643 1107 36-37 -0.8114232217064412 1107 38-39 -0.8382398742648007 1107 40-41 -0.8681202419800655 1107 42-43 -0.716317931930071 1107 44-45 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0.7186247585975707 2311 30-31 0.9407631880549943 2311 32-33 1.0098962098953805 2311 34-35 1.0248246883845766 2311 36-37 1.1002487951174018 2311 38-39 1.0743398592020554 2311 40-41 1.0678500060051377 2311 42-43 1.0565078770798664 2311 44-45 1.0461454174710951 2311 46-47 0.6908937821270555 2311 48-49 0.9601556233281983 2311 50-51 1.0574448870721582 2311 52-53 0.9839391038262306 2311 54-55 1.061595217769927 2311 56-57 1.0722011100930189 2311 58-59 1.09129578816367 2311 60-61 1.1181963467954361 2311 62-63 1.1491029756249205 2311 64-65 1.14070760773253 2311 66-67 1.1512020160823155 2311 68-69 1.1851685151447526 2311 70-71 1.1870367858451587 2311 72-73 1.1732950685859578 2311 74-75 1.1762544712465584 2311 76-77 1.17929394028263 2311 78-79 0.8902017420062904 2311 80-81 0.8622709342806587 2311 82-83 0.9588136139329038 2311 84-85 1.02693198526606 2311 86-87 1.0298469387465836 2311 88-89 1.0976194228055753 2311 90-91 1.139330264307933 2311 92-93 1.2524932764880354 2311 94-95 1.2499617104030953 2311 96-97 1.2565412982284805 2311 98-99 1.2916623414837325 2311 100 1.4163508940147658 2312 1 0.2526134736349519 2312 2 0.3774373004824554 2312 3 0.4492035545072639 2312 4 0.18109205742968726 2312 5 0.3218802016211555 2312 6 0.4360940007602707 2312 7 0.25605170859913784 2312 8 0.4127483140946069 2312 9 0.5943671585138617 2312 10-11 0.6385494220830452 2312 12-13 0.7100285014447962 2312 14-15 0.8887644727139588 2312 16-17 0.8884800475105408 2312 18-19 0.8535132346988874 2312 20-21 0.8779823346452957 2312 22-23 0.9163955222848514 2312 24-25 0.9079341932609353 2312 26-27 0.9779057573585419 2312 28-29 0.9618893603814556 2312 30-31 0.9579409842777338 2312 32-33 0.8324604866631731 2312 34-35 0.9505222642742623 2312 36-37 0.9933616887505607 2312 38-39 0.8241738511946437 2312 40-41 0.8880712804861801 2312 42-43 0.9747740099113074 2312 44-45 0.6885840972896773 2312 46-47 0.7300813066991907 2312 48-49 0.9143251785396345 2312 50-51 0.7590944838654679 2312 52-53 0.9277356747457404 2312 54-55 0.7596009925332581 2312 56-57 0.8122537374551158 2312 58-59 0.9336975022776173 2312 60-61 0.9640662238034423 2312 62-63 1.0842494613752578 2312 64-65 1.0459464839686987 2312 66-67 0.9703264118684629 2312 68-69 1.0391752732648527 2312 70-71 0.9467781333483174 2312 72-73 0.9748122070128105 2312 74-75 1.0287586653148395 2312 76-77 1.0598981650204813 2312 78-79 0.9574190075055 2312 80-81 0.9164901530046308 2312 82-83 0.9284885764355053 2312 84-85 0.981838797165068 2312 86-87 0.9363972419254232 2312 88-89 0.9721482273197104 2312 90-91 1.0088329520175172 2312 92-93 1.0655108988527893 2312 94-95 1.1503354988618533 2312 96-97 0.8270194445131231 2312 98-99 0.6679122723131137 2312 100 0.8937647364147772 2313 1 0.07019114929001091 2313 2 0.2212570740522395 2313 3 0.27274787925543365 2313 4 0.17231705369715655 2313 5 0.30062121599872427 2313 6 0.2631047139943874 2313 7 0.242131101499524 2313 8 0.38426103870583717 2313 9 0.5976829349541859 2313 10-11 0.3976631127367156 2313 12-13 0.45484307992306583 2313 14-15 0.42595385087697224 2313 16-17 0.718162971761906 2313 18-19 0.6899964262295839 2313 20-21 0.6453939777534288 2313 22-23 0.628623229042482 2313 24-25 0.5068999125245099 2313 26-27 0.7346513372156735 2313 28-29 0.5833274173945426 2313 30-31 0.7943229290288301 2313 32-33 0.8852186813704748 2313 34-35 0.9328801416675461 2313 36-37 0.9551564575608396 2313 38-39 0.9421616295579014 2313 40-41 0.957228931389082 2313 42-43 0.9976480139176189 2313 44-45 0.8733245673619408 2313 46-47 0.8950992557952233 2313 48-49 0.9103489075967417 2313 50-51 0.8742965830760099 2313 52-53 0.9413927868381222 2313 54-55 0.8493241044180522 2313 56-57 0.5766320704663883 2313 58-59 0.6428791684812794 2313 60-61 0.5704861538276518 2313 62-63 0.9406756703148815 2313 64-65 1.0255702945428666 2313 66-67 1.0368710889029984 2313 68-69 1.1156797613785798 2313 70-71 1.0682833741711733 2313 72-73 1.0878968839043281 2313 74-75 1.1607090574825563 2313 76-77 1.095599844000116 2313 78-79 0.9764257847900737 2313 80-81 0.8973902979674051 2313 82-83 0.9600129684770309 2313 84-85 0.9468867990345302 2313 86-87 0.9610254449900673 2313 88-89 1.0350508964181877 2313 90-91 0.8833684073675734 2313 92-93 1.0374704154022858 2313 94-95 1.204329964030105 2313 96-97 1.3026678063136679 2313 98-99 1.2854260130436224 2313 100 1.3505242451650723 2314 1 -0.44384351057220073 2314 2 -0.012576184203339835 2314 3 0.24914736104460644 2314 4 0.2103333351323471 2314 5 0.3068053422005974 2314 6 0.3710258856763531 2314 7 0.4116514113085685 2314 8 0.4134818491942056 2314 9 0.394524168265761 2314 10-11 0.5133404440114759 2314 12-13 0.4485470073052511 2314 14-15 0.7598913443107236 2314 16-17 0.8088301079680136 2314 18-19 0.8212189115822284 2314 20-21 0.8528780245406011 2314 22-23 0.9205897709459592 2314 24-25 0.8213498470485945 2314 26-27 0.7163810510368265 2314 28-29 0.737295189230089 2314 30-31 0.7917581370562701 2314 32-33 0.8845941510762714 2314 34-35 0.9500617764216486 2314 36-37 0.8796348217226537 2314 38-39 0.6271968933980361 2314 40-41 0.6402807174823835 2314 42-43 0.8203456332840915 2314 44-45 0.8765127275352853 2314 46-47 0.8365460508376188 2314 48-49 0.8731126238458771 2314 50-51 0.8440090743733961 2314 52-53 0.8436523791340989 2314 54-55 0.8106920716716814 2314 56-57 0.7926367214634382 2314 58-59 0.8723945046455057 2314 60-61 0.955841814304776 2314 62-63 0.9063462741042301 2314 64-65 0.9088610703273403 2314 66-67 0.6076366646626994 2314 68-69 0.614951468222408 2314 70-71 0.7449563747839605 2314 72-73 0.8272910065903503 2314 74-75 0.9435067972959743 2314 76-77 0.8051824387952813 2314 78-79 0.6937100243397722 2314 80-81 0.7556563122447955 2314 82-83 0.7737236620043788 2314 84-85 0.8385735502616356 2314 86-87 0.8729468718953619 2314 88-89 0.7973961313179423 2314 90-91 0.9344437539731523 2314 92-93 0.8086149728406937 2314 94-95 0.842539231214328 2314 96-97 0.8355900195650108 2314 98-99 1.0496546188201883 2314 100 1.0270184329662975 2315 1 0.3793425200308036 2315 2 0.4150203379424724 2315 3 0.43759169551517374 2315 4 0.3235378200944439 2315 5 0.36658238722446157 2315 6 0.41760747451245805 2315 7 0.3461735752638546 2315 8 0.24482099018177905 2315 9 0.47036408923001005 2315 10-11 0.5211863269470456 2315 12-13 0.38360564488822746 2315 14-15 0.5903602927977261 2315 16-17 0.7691623722721346 2315 18-19 0.7359577899408052 2315 20-21 0.46701472340609484 2315 22-23 0.7974192635291288 2315 24-25 0.8352869163935921 2315 26-27 0.7825319717666943 2315 28-29 0.7868324977705825 2315 30-31 0.8430862790831171 2315 32-33 0.7576192548374152 2315 34-35 0.7974119036434857 2315 36-37 0.8547918985485339 2315 38-39 0.896478851308089 2315 40-41 0.9075697344061595 2315 42-43 0.9278295039652704 2315 44-45 0.9313359625961652 2315 46-47 0.9240867840987192 2315 48-49 0.8203180812552517 2315 50-51 0.8734374418020181 2315 52-53 0.900638193498736 2315 54-55 0.7606838808766767 2315 56-57 0.6682782165136416 2315 58-59 0.7173322035422629 2315 60-61 0.837074633750511 2315 62-63 0.9439289256063077 2315 64-65 0.9566310191591612 2315 66-67 0.946003472092066 2315 68-69 1.0301379046455992 2315 70-71 0.8948236009412156 2315 72-73 0.9666252984247663 2315 74-75 0.36729500969341444 2315 76-77 0.3840864817145011 2315 78-79 0.7120470720819938 2315 80-81 0.7610455751674294 2315 82-83 0.7589658590632098 2315 84-85 0.7710487260081749 2315 86-87 0.8000185537415341 2315 88-89 0.7686222248935017 2315 90-91 0.7640767425520529 2315 92-93 0.8619588190894731 2315 94-95 0.9084452537036931 2315 96-97 1.0621176845310565 2315 98-99 1.0535628330814895 2315 100 0.968342894960692 2316 1 0.27224365812858053 2316 2 0.10019670214713017 2316 3 0.22237821373381905 2316 4 0.04263312512771478 2316 5 0.2641199073446714 2316 6 0.36192821784458573 2316 7 0.41737574571164515 2316 8 0.4287017158409938 2316 9 0.6290340520177224 2316 10-11 0.626092137335398 2316 12-13 0.36034808445237587 2316 14-15 0.4656254004370268 2316 16-17 0.7365132375596346 2316 18-19 0.7137470877990566 2316 20-21 0.7182095617117454 2316 22-23 0.775534543635338 2316 24-25 0.5090176980746008 2316 26-27 0.749691660919531 2316 28-29 0.8800942285015552 2316 30-31 0.5315862765759292 2316 32-33 0.6885173698194507 2316 34-35 0.8747117503705155 2316 36-37 0.8929959025890142 2316 38-39 0.8212175824574928 2316 40-41 0.8448138601958561 2316 42-43 0.7997279214817397 2316 44-45 0.6502326841368955 2316 46-47 0.7849289079238915 2316 48-49 0.8811391140286986 2316 50-51 0.9545779464829494 2316 52-53 0.932612096978545 2316 54-55 0.7714620629622928 2316 56-57 0.9167865740970882 2316 58-59 0.7933018709828161 2316 60-61 0.8909420712949796 2316 62-63 0.8890846667683121 2316 64-65 0.9269368045870223 2316 66-67 0.9098692938909281 2316 68-69 0.8985959150387473 2316 70-71 0.8809403430186293 2316 72-73 0.9124492005395552 2316 74-75 0.8960323546445181 2316 76-77 0.7785460092370577 2316 78-79 0.5792402433839001 2316 80-81 0.6330708537860446 2316 82-83 0.5464415741281456 2316 84-85 0.681080057544122 2316 86-87 0.7347529474497172 2316 88-89 0.6809451544965803 2316 90-91 0.5605129509654141 2316 92-93 0.35482071685512295 2316 94-95 0.4146279794888237 2316 96-97 0.6299957620389272 2316 98-99 0.8260050910692662 2316 100 0.46182392972875164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 35.0 13 253.0 14 945.0 15 2562.0 16 6038.0 17 12486.0 18 21495.0 19 32525.0 20 48338.0 21 70402.0 22 85947.0 23 86262.0 24 85645.0 25 92906.0 26 110051.0 27 135372.0 28 168993.0 29 208057.0 30 257023.0 31 323092.0 32 412916.0 33 550732.0 34 806750.0 35 1127498.0 36 1792932.0 37 2526413.0 38 1887517.0 39 323520.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.517472999772195 21.83704935656413 13.580518212817875 23.064959430845803 2 15.195009201708299 19.987243137057362 41.40572791914342 23.412019742090912 3 18.78045893391528 26.694411218165886 28.889239418795963 25.635890429122867 4 12.27645245990789 16.531565183403966 39.75988813706378 31.43209421962436 5 12.854574893960477 38.85472216423965 34.60545567491168 13.685247266888192 6 29.832055514632234 38.42632312190803 17.65300921226595 14.088612151193788 7 27.1764605778437 32.164130822723614 22.96641355200288 17.69299504742981 8 26.591241498295254 35.620117478020624 19.30969248695213 18.47894853673199 9 26.33729191156673 15.235474960039738 21.822209468105925 36.605023660287614 10-11 26.209626496088934 26.299769515706515 26.61616480416574 20.874439184038806 12-13 25.87257200855206 23.83948593899753 27.767621257312513 22.520320795137895 14-15 23.36058016341656 25.08399383611264 25.369610009669213 26.185815990801586 16-17 21.64162245042118 27.825599490511465 26.792190610125584 23.740587448941767 18-19 21.016372500397924 27.924565407285083 29.278262003131683 21.780800089185306 20-21 22.73109113249344 26.99516146363779 28.663914757871424 21.609832645997347 22-23 23.126998963650276 26.76469117216674 28.297975194710368 21.810334669472617 24-25 22.41878741974186 26.604506234660853 29.28701798983874 21.68968835575854 26-27 22.3796298354981 26.957559264359332 28.11680785872047 22.546003041422097 28-29 21.50769544195617 27.39753058893386 28.8052431513074 22.289530817802568 30-31 22.562515733422067 26.971506551747016 28.74979985812127 21.716177856709642 32-33 22.887546949304706 27.15472392388245 27.857478588835168 22.10025053797768 34-35 22.77201631668097 27.054449241763162 28.666495401687452 21.50703903986842 36-37 22.313465690410382 26.58708248938371 28.361871517267407 22.737580302938497 38-39 22.55828781119337 26.745030032061074 28.958197817702597 21.73848433904296 40-41 22.120686522853784 25.78763000339057 29.62733200176959 22.46435147198605 42-43 22.234382417062104 25.962465826852714 28.83018065718109 22.972971098904093 44-45 22.29392247415666 26.91907863662531 28.615424840882397 22.17157404833564 46-47 22.353252068370097 26.75283199372009 27.8938892257367 23.000026712173113 48-49 23.21073196657579 26.741604802291985 27.893507976231913 22.154155254900314 50-51 22.692945592119184 26.380384045628404 27.984066924999745 22.942603437252668 52-53 22.05807135829009 27.080959836041224 27.64026407070042 23.220704734968265 54-55 22.009584020303553 27.02294730703722 27.580587193605986 23.386881479053237 56-57 23.140236583735934 26.455144046839685 27.206346752259194 23.19827261716519 58-59 21.91570753006104 27.088434887794026 28.04235557272174 22.953502009423197 60-61 22.16310598787977 27.758902338211232 27.24939520997893 22.82859646393007 62-63 22.63139663459377 26.871377941918766 26.853671258101937 23.643554165385527 64-65 21.7123803359142 27.136728575118678 27.249288312063996 23.90160277690313 66-67 22.247465152789136 26.744746418590527 27.323520417006268 23.684268011614066 68-69 21.81754008387634 26.734409040632446 27.57541059703143 23.872640278459787 70-71 21.76931173166704 26.336643944678574 27.689400068347236 24.20464425530715 72-73 22.358353476651356 26.50730261666803 27.84135807795688 23.292985828723733 74-75 22.617628085431832 26.880179865910574 27.31247651935834 23.189715529299253 76-77 22.307682600406025 26.768697336638027 26.939418210113136 23.984201852842812 78-79 22.254211752194774 26.796032988067438 26.958730821774175 23.991024437963617 80-81 21.734851500146267 27.374275533954062 27.465197691944333 23.425675273955342 82-83 22.833753177924006 26.184290234102146 28.578952861286233 22.403003726687615 84-85 22.995323386540605 26.720066701853312 27.16439063481344 23.12021927679264 86-87 21.49869577777919 27.494375913754553 27.68008781175083 23.326840496715427 88-89 22.559875425021147 26.229929009739223 27.518933004418717 23.69126256082091 90-91 22.25588582256815 26.29702171575243 27.58272090540464 23.86437155627478 92-93 22.675076376231125 26.51722685450225 27.343388895631744 23.464307873634883 94-95 21.45987313275004 26.508505489084357 28.081064501209795 23.950556876955805 96-97 21.87107722177189 26.474452369590125 27.57103965570749 24.083430752930497 98-99 21.156307361709704 26.972665589001476 27.492454240619672 24.378572808669148 100 21.859790023620395 26.39442096466553 26.740407721455643 25.005381290258427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 794.0 1 557.0 2 417.5 3 537.5 4 474.0 5 431.0 6 737.0 7 1458.5 8 2389.0 9 2988.5 10 2923.5 11 2883.0 12 3413.0 13 4334.0 14 5581.5 15 7031.5 16 8479.5 17 9866.5 18 11307.0 19 12645.5 20 13906.5 21 15208.0 22 16835.0 23 18903.0 24 21024.5 25 23981.5 26 29077.0 27 35063.0 28 43588.5 29 61887.0 30 89669.0 31 116247.5 32 154310.0 33 199153.0 34 233584.5 35 269818.0 36 304862.0 37 356535.5 38 380877.5 39 363143.5 40 333707.0 41 299711.0 42 292901.5 43 309479.0 44 339787.0 45 405068.5 46 465550.0 47 502267.5 48 531775.5 49 525429.5 50 500140.0 51 508435.5 52 510783.0 53 477601.5 54 447577.0 55 386045.0 56 330333.5 57 269145.5 58 207696.0 59 200991.0 60 164428.5 61 105097.0 62 74486.0 63 63233.5 64 45516.0 65 19185.5 66 9501.0 67 6294.0 68 4635.0 69 3570.5 70 2681.0 71 1903.0 72 1427.5 73 1187.5 74 903.5 75 594.5 76 385.0 77 268.5 78 178.5 79 107.0 80 66.0 81 38.0 82 25.0 83 20.0 84 14.5 85 6.5 86 4.0 87 5.0 88 5.5 89 4.5 90 2.0 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04530842610570588 2 0.0 3 0.0 4 0.033730799055788144 5 0.0 6 0.0 7 9.662934477520211E-4 8 0.0 9 0.011201846264680838 10-11 4.0709585067330524E-4 12-13 8.947161553259456E-6 14-15 1.431545848521513E-4 16-17 2.6841484659778365E-4 18-19 0.0 20-21 1.789432310651891E-5 22-23 0.0 24-25 5.234089508656781E-4 26-27 4.42884496886343E-4 28-29 1.3868100407552156E-4 30-31 0.002527573138795796 32-33 0.015894632499365423 34-35 0.02693542985608759 36-37 0.03194136674513626 38-39 0.02315078051905884 40-41 0.0013018120059992506 42-43 6.710371164944591E-5 44-45 0.01134947443030962 46-47 0.018328260441851995 48-49 0.003569917459750523 50-51 0.011040797356722167 52-53 0.011067638841381946 54-55 0.010266867882365225 56-57 4.652524007694917E-4 58-59 0.009032159588015419 60-61 0.004795678592547068 62-63 0.0014181251061916238 64-65 0.03574391040527153 66-67 0.061064377600995784 68-69 0.060071242668583984 70-71 0.05217537259783251 72-73 0.04578262566802863 74-75 0.03544418049323733 76-77 0.03149400866747328 78-79 0.015375697129276373 80-81 0.0012123403904666563 82-83 0.0388396283026993 84-85 0.05211721604773632 86-87 0.05788813524958868 88-89 0.0704410029088117 90-91 0.048444406230123326 92-93 0.0394122466421079 94-95 0.01346100455687885 96-97 0.003744387110039082 98-99 0.02301209951498332 100 0.033623433117149035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1176729E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.24803027900922 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89706154184678 43.91904371023983 2 15.731459484469823 18.955788947066896 3 5.52463237122314 9.985446551455388 4 2.4590729448525406 5.926172049590731 5 1.2570550417051762 3.786754510751733 6 0.6827015088801065 2.4678852707120362 7 0.4250431189196549 1.7925607488989124 8 0.258860663382941 1.2476676068431876 9 0.17195545549105287 0.9323979740159197 >10 0.5466705988099871 5.151667644492573 >50 0.0229082737270819 0.9618096634900171 >100 0.01962930563529032 2.3372603227577864 >500 0.002083529579819185 0.8815196395500152 >1k 7.836699348857134E-4 0.7084048440058901 >5k 2.0622885475772024E-5 0.08096053297458898 >10k+ 6.186865642731607E-5 0.8646599831544847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29701 0.2657396452933591 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24850 0.22233696459849744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15040 0.1345653097610222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.8418777085854E-5 0.0 0.0 0.0 6 0.0 9.8418777085854E-5 0.0 0.0 0.0 7 0.0 1.1631310019237292E-4 0.0 0.0 0.0 8 0.0 1.1631310019237292E-4 0.0 0.0 0.0 9 0.0 1.1631310019237292E-4 0.0 0.0 0.0 10-11 0.0 1.3420742329889183E-4 0.0 0.0 0.0 12-13 0.0 1.431545848521513E-4 0.0 0.0 0.0 14-15 0.0 1.431545848521513E-4 0.0 8.947161553259456E-6 0.0 16-17 0.0 1.431545848521513E-4 0.0 4.026222698966755E-5 0.0 18-19 0.0 1.5210174640541073E-4 0.0 7.157729242607565E-5 0.0 20-21 0.0 1.610489079586702E-4 0.0 1.5210174640541076E-4 0.0 22-23 0.0 1.610489079586702E-4 0.0 3.0420349281082146E-4 0.0 24-25 0.0 1.610489079586702E-4 0.0 7.202465050373862E-4 0.0 26-27 0.0 1.610489079586702E-4 0.0 0.0016060154988100724 0.0 28-29 0.0 1.789432310651891E-4 0.0 0.004733048461674252 0.0 30-31 0.0 1.789432310651891E-4 0.0 0.01859220170767315 0.0 32-33 0.0 1.789432310651891E-4 0.0 0.0519785350436608 0.0 34-35 0.0 1.9236397339507827E-4 0.0 0.10015452642718634 0.0 36-37 0.0 2.2815261960811614E-4 0.0 0.18240578258630052 0.0 38-39 0.0 2.505205234912648E-4 0.0 0.30159092163726975 0.0 40-41 0.0 2.505205234912648E-4 0.0 0.42311126985363967 0.0 42-43 0.0 2.505205234912648E-4 0.0 0.5436340095568211 0.0 44-45 0.0 2.549941042678945E-4 0.0 0.6829099998756345 0.0 46-47 0.0 2.594676850445242E-4 0.0 0.8494793065126658 0.0 48-49 0.0 2.594676850445242E-4 0.0 1.0346005526303805 0.0 50-51 0.0 2.594676850445242E-4 0.0 1.2256671876002363 0.0 52-53 0.0 2.6841484659778365E-4 0.0 1.4063238001028746 0.0 54-55 0.0 3.6236004290700796E-4 0.0 1.5989696090868804 0.0 56-57 0.0 4.294637545564539E-4 0.0 1.8240399315398985 0.0 58-59 0.0 5.189353700890484E-4 0.0 2.083914712435096 0.0 60-61 0.0 5.323561124189375E-4 0.0 2.346549692669474 0.0 62-63 0.0 5.457768547488268E-4 0.0 2.613201948441266 0.0 64-65 0.0 5.726183394086052E-4 0.0 2.8807936561761496 0.0 66-67 0.0 5.905126625151241E-4 0.0 3.1777410009672775 0.0 68-69 0.0 5.905126625151241E-4 0.0 3.5039187225529043 4.473580776629728E-6 70-71 0.0 5.994598240683835E-4 0.0 3.860234957830685 8.947161553259456E-6 72-73 0.0 6.084069856216429E-4 0.0 4.217696429787283 8.947161553259456E-6 74-75 0.0 6.084069856216429E-4 0.0 4.557701989553474 8.947161553259456E-6 76-77 0.0 6.173541471749024E-4 0.0 4.939835259493185 8.947161553259456E-6 78-79 0.0 6.173541471749024E-4 0.0 5.350272875006633 8.947161553259456E-6 80-81 0.0 6.218277279515321E-4 0.0 5.785892276711729 8.947161553259456E-6 82-83 0.0 6.263013087281619E-4 0.0 6.2273407541687735 8.947161553259456E-6 84-85 0.0 6.263013087281619E-4 0.0 6.64363428691883 8.947161553259456E-6 86-87 0.0 6.531427933879402E-4 0.0 7.085158815249077 8.947161553259456E-6 88 0.0 6.531427933879402E-4 0.0 7.4397706162509625 8.947161553259456E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21420 0.0 43.81574 1 GTATCAA 37225 0.0 38.53866 1 GGGCACC 12560 0.0 38.101856 94 GTACTAG 12685 0.0 37.62397 1 TAGGCAT 15455 0.0 33.928413 5 CAACGCA 41460 0.0 32.54794 5 CTGTGCG 16360 0.0 32.53995 9 ATCAACG 42230 0.0 31.999022 3 TAGTACT 17625 0.0 30.828234 4 ACTGTGC 18020 0.0 30.793814 8 CTAGGCA 17600 0.0 30.337906 4 TATCAAC 47510 0.0 30.121832 2 TACCTGG 4940 0.0 29.682928 2 GCATCGT 15445 0.0 29.356735 8 TACCCCG 645 0.0 29.138594 5 GTACTGT 18960 0.0 28.87061 6 AACGCAG 48880 0.0 28.58763 6 TACTGTG 20620 0.0 28.437689 7 GTGGTAT 6745 0.0 28.303047 1 AAGCTAA 7185 0.0 27.465837 9 >>END_MODULE