FastQCFastQC Report
Mon 6 Jun 2016
ERR1211206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13006869
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323330.24858403663479658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261900.2013551455004275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146990.11300951827838043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216700.044.282031
GTATCAA366050.038.325761
GTACTAG107900.034.7184871
CAACGCA399050.033.301435
ATCAACG407750.032.5677073
TATCAAC450100.030.9595932
AACGCAG451200.030.2234956
GCATCGT110150.029.9149238
TCAACGC473550.028.3799114
TAGGCAT133500.028.3451655
CTGTGCG144100.027.9229189
GGGCACC127550.027.78284694
TAGTACT152200.027.3950234
GTGGTAT77800.027.2208731
ACGCAGA489600.026.9893347
CGCAGAG498550.026.4848428
GTACATG495600.026.303751
CTAGGCA149850.026.1306954
CTAGTAC167050.026.0008893
TACATGG499150.025.7016282
Produced by FastQC (version 0.11.5)