FastQCFastQC Report
Mon 6 Jun 2016
ERR1211206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13006869
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293450.22561155955364817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254990.19604256796927838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147540.11343237177217667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217750.044.4377441
GTATCAA361850.036.9023971
GTACTAG108000.032.5208361
CAACGCA401400.031.5942525
ATCAACG409400.030.9980053
GGGCACC120350.030.15568594
TATCAAC454800.029.2543532
AACGCAG456700.028.4270926
GTGGTAT75600.028.2979161
CTGTGCG145250.027.8850889
TCAACGC474900.027.0888424
TAGGCAT134450.027.0147695
TAGTACT154850.026.8930684
GTACATG490550.026.099321
ACGCAGA493550.025.7714987
TACATGG496950.025.3450492
CGCAGAG504400.025.1238848
CTAGGCA151000.024.995194
CTAGTAC172050.024.9694733
CTAACGC45850.024.6030943
Produced by FastQC (version 0.11.5)