FastQCFastQC Report
Mon 6 Jun 2016
ERR1211205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5764090
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200650.3481035167736798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156110.2708319960305963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97840.169740583509279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.049.215561
TACCTGG55300.044.168472
GTATCAA162400.039.585721
GTACCTG66800.037.4103621
ACCTGGG69350.034.4350553
CAACGCA192750.032.217515
ATCAACG192950.032.208483
TATCAAC200100.031.6568782
GTACATG184400.030.1924041
AACGCAG211600.029.9697866
TCAACGC208600.029.9267654
GTGGTAT39800.029.7982921
TACATGG188000.028.988172
ACATGGG189300.027.8628653
CCTGGGG67350.027.1511364
TATCACG4900.026.8925552
CATGGGG152550.026.7783364
GTACTAG27250.026.7694281
ACGCAGA232050.026.6398337
ATAACGC6600.026.3536853
Produced by FastQC (version 0.11.5)