##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211205_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5764090 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.420479555315758 31.0 31.0 34.0 28.0 34.0 2 31.419899411702453 31.0 31.0 34.0 28.0 34.0 3 31.483853132064212 33.0 31.0 34.0 28.0 34.0 4 35.0820230079683 37.0 35.0 37.0 32.0 37.0 5 35.14082552493108 37.0 35.0 37.0 32.0 37.0 6 35.202206245912194 37.0 35.0 37.0 32.0 37.0 7 35.12053229564424 37.0 35.0 37.0 32.0 37.0 8 35.123609624416 37.0 35.0 37.0 32.0 37.0 9 36.69259761731687 39.0 37.0 39.0 32.0 39.0 10-11 36.63802621055535 39.0 37.0 39.0 32.0 39.0 12-13 36.5351336464212 39.0 37.0 39.0 32.0 39.0 14-15 37.73313645345579 40.0 37.0 41.0 32.0 41.0 16-17 37.70277572001825 40.0 37.0 41.0 32.0 41.0 18-19 37.711911073560614 40.0 37.0 41.0 32.0 41.0 20-21 37.674536830618536 40.0 37.0 41.0 32.0 41.0 22-23 37.56672761875682 40.0 37.0 41.0 32.0 41.0 24-25 37.47641119413472 40.0 37.0 41.0 31.5 41.0 26-27 37.33094200472234 40.0 37.0 41.0 31.0 41.0 28-29 37.201629398569416 40.0 36.0 41.0 31.0 41.0 30-31 37.041368542128936 39.5 36.0 41.0 30.0 41.0 32-33 36.83685473335774 39.0 35.5 41.0 30.0 41.0 34-35 36.679084035814846 39.0 35.0 41.0 30.0 41.0 36-37 36.56879047690095 39.0 35.0 41.0 30.0 41.0 38-39 36.42682262074326 39.0 35.0 41.0 29.0 41.0 40-41 36.264303211781915 39.0 35.0 40.5 29.0 41.0 42-43 36.06251090805314 39.0 35.0 40.0 28.0 41.0 44-45 35.86333471545378 38.5 35.0 40.0 27.0 41.0 46-47 35.64098322545276 38.0 34.5 40.0 26.5 41.0 48-49 35.44686672137319 38.0 34.0 40.0 26.0 41.0 50-51 34.77287021888971 37.5 33.5 39.5 25.0 40.5 52-53 34.884398404605065 38.0 33.5 40.0 25.5 40.5 54-55 35.37929924758288 38.0 34.0 40.0 26.0 41.0 56-57 35.353308154452826 38.0 34.0 40.0 26.0 41.0 58-59 35.16119968633384 38.0 34.0 40.0 26.0 41.0 60-61 34.933575811619875 37.5 34.0 40.0 26.0 41.0 62-63 34.63421494112687 37.0 33.5 40.0 26.0 41.0 64-65 34.38169095208437 36.5 33.0 40.0 25.0 41.0 66-67 34.19994734641548 36.0 33.0 39.0 25.0 41.0 68-69 33.898415448058586 36.0 33.0 39.0 25.0 41.0 70-71 33.57269074563374 35.0 33.0 39.0 24.0 40.5 72-73 33.22144579630089 35.0 32.0 38.0 24.0 40.0 74-75 32.88937178288333 35.0 32.0 37.0 23.5 39.5 76-77 32.54132603758789 35.0 32.0 37.0 23.0 39.0 78-79 32.199017364406174 35.0 31.0 36.0 22.0 39.0 80-81 31.902245540926668 35.0 31.0 36.0 21.5 37.5 82-83 31.607199575301564 35.0 31.0 35.0 20.0 37.0 84-85 31.354671162317036 34.5 31.0 35.0 20.0 37.0 86-87 31.12569633714949 34.0 31.0 35.0 20.0 36.0 88-89 30.906518808693132 34.0 30.0 35.0 19.5 36.0 90-91 30.68222711650928 34.0 30.0 35.0 19.0 36.0 92-93 30.488775765125112 34.0 30.0 35.0 18.0 35.0 94-95 30.3059012784325 34.0 29.0 35.0 18.0 35.0 96-97 30.12151874797236 34.0 29.0 35.0 17.5 35.0 98-99 29.885628433976567 34.0 29.0 35.0 16.5 35.0 100 28.77099195189527 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5462218694099725 1101 2 -0.48156123382208804 1101 3 -0.5211095723971653 1101 4 -0.501716715391261 1101 5 -0.461788513420089 1101 6 -0.38397606174541465 1101 7 -0.3953093488518107 1101 8 -0.3649822287563609 1101 9 -0.5522464097587658 1101 10-11 -0.6268530936331729 1101 12-13 -0.6242531263056037 1101 14-15 -0.8143928102732403 1101 16-17 -0.919306677141897 1101 18-19 -0.7497958142113035 1101 20-21 -0.79085790042285 1101 22-23 -0.8952412450140628 1101 24-25 -0.9435446210891598 1101 26-27 -0.9206290904897969 1101 28-29 -0.8913453787044645 1101 30-31 -0.9636948453270122 1101 32-33 -0.9298647749712927 1101 34-35 -1.008384751815143 1101 36-37 -1.0582501639726942 1101 38-39 -1.1286235175533292 1101 40-41 -1.086181251082337 1101 42-43 -1.1397173644988357 1101 44-45 -1.0499961735504826 1101 46-47 -1.2904470405234036 1101 48-49 -1.333679523790984 1101 50-51 -1.3716921136828546 1101 52-53 -1.2342074481544927 1101 54-55 -1.0237506099892073 1101 56-57 -0.9561693358214214 1101 58-59 -0.9831639141232742 1101 60-61 -0.9722884477511755 1101 62-63 -0.9821077131827209 1101 64-65 -0.924707339150153 1101 66-67 -0.9762623639384032 1101 68-69 -0.9540526335776889 1101 70-71 -0.8999600828442738 1101 72-73 -1.0480372401534694 1101 74-75 -1.101593666801996 1101 76-77 -1.2000047542182202 1101 78-79 -1.0917632143182487 1101 80-81 -1.2121035884480165 1101 82-83 -1.140836165468123 1101 84-85 -1.2461532245900102 1101 86-87 -1.2782277029630933 1101 88-89 -1.4846025136276282 1101 90-91 -1.5280743740629177 1101 92-93 -1.4930324048062893 1101 94-95 -1.6242749941175383 1101 96-97 -1.5934887176993904 1101 98-99 -1.7222013569826977 1101 100 -1.7548918685988077 1102 1 -0.42659742325126615 1102 2 -0.31347272794103986 1102 3 -0.3615397143880763 1102 4 -0.21862589695692236 1102 5 -0.3617606381985823 1102 6 -0.34389286645506445 1102 7 -0.30536833276389075 1102 8 -0.3360015190042418 1102 9 -0.5225942313647138 1102 10-11 -0.5451014325516965 1102 12-13 -0.5689774397986369 1102 14-15 -0.5973330508382446 1102 16-17 -0.6978181693354912 1102 18-19 -0.7100181456472612 1102 20-21 -0.7036036260216676 1102 22-23 -0.7437171010941057 1102 24-25 -0.6994673270214307 1102 26-27 -0.75999485275252 1102 28-29 -0.7684756777394739 1102 30-31 -0.7714948568225424 1102 32-33 -0.868395912188312 1102 34-35 -0.8146509005793519 1102 36-37 -0.8520460089083102 1102 38-39 -1.024395052436077 1102 40-41 -1.079781505824208 1102 42-43 -0.9885057904350489 1102 44-45 -0.9590508743901793 1102 46-47 -0.8430404802054028 1102 48-49 -1.0374915452740154 1102 50-51 -1.0132797993755034 1102 52-53 -0.9213208458013895 1102 54-55 -0.8756429680305047 1102 56-57 -0.9017456379758002 1102 58-59 -0.8395467806445609 1102 60-61 -0.8461832957335389 1102 62-63 -0.9325339888846571 1102 64-65 -0.9564153682190977 1102 66-67 -1.0368316880853214 1102 68-69 -1.1274138246721677 1102 70-71 -1.0483990173510946 1102 72-73 -1.0871406617555053 1102 74-75 -1.0364772767805022 1102 76-77 -1.1554226447024405 1102 78-79 -1.0362802332806176 1102 80-81 -1.1213683444421463 1102 82-83 -1.0682452096396595 1102 84-85 -1.113730385086022 1102 86-87 -1.1442836181941054 1102 88-89 -1.138893351145665 1102 90-91 -1.165337489538679 1102 92-93 -1.1937156308740562 1102 94-95 -1.2868626209168816 1102 96-97 -1.290403019868208 1102 98-99 -1.4366078561422668 1102 100 -1.4184167802553098 1103 1 -0.450649014773397 1103 2 -0.3893213508458899 1103 3 -0.5294691623391508 1103 4 -0.42804838531156975 1103 5 -0.3435950641649228 1103 6 -0.39731896810970113 1103 7 -0.3414578709798377 1103 8 -0.39640489756933306 1103 9 -0.6696092120912169 1103 10-11 -0.5853924463346587 1103 12-13 -0.7038613695103209 1103 14-15 -0.838450595721504 1103 16-17 -0.7971148698254282 1103 18-19 -0.6924261132498444 1103 20-21 -0.7354358648201043 1103 22-23 -0.7484453636213146 1103 24-25 -0.8177939731153359 1103 26-27 -0.8631805561270554 1103 28-29 -0.9234048603423588 1103 30-31 -0.8334661987947953 1103 32-33 -0.8898989248095717 1103 34-35 -0.9886387507736316 1103 36-37 -0.8737826892495306 1103 38-39 -0.9713367894531189 1103 40-41 -1.0921778522042587 1103 42-43 -1.0884127847303162 1103 44-45 -1.1141863058690902 1103 46-47 -1.1804648741080683 1103 48-49 -1.1970425552771573 1103 50-51 -1.221260324212686 1103 52-53 -1.1545751522856662 1103 54-55 -0.9647229705466316 1103 56-57 -0.9138604877469447 1103 58-59 -0.9331348672643003 1103 60-61 -0.9392838129688101 1103 62-63 -0.8666108465985047 1103 64-65 -0.8589071617608752 1103 66-67 -0.9540798032995497 1103 68-69 -0.8888213140563082 1103 70-71 -0.9004931619328218 1103 72-73 -0.8801249209793482 1103 74-75 -0.8967507953334959 1103 76-77 -0.8499413272370262 1103 78-79 -0.8660697558799804 1103 80-81 -0.9241386303187262 1103 82-83 -0.8430428508231067 1103 84-85 -0.982666708633591 1103 86-87 -1.0505299971280913 1103 88-89 -0.9641993556576409 1103 90-91 -1.2039266507235098 1103 92-93 -1.2932405927993678 1103 94-95 -1.321035428735403 1103 96-97 -1.3142554417533852 1103 98-99 -1.2765187006983076 1103 100 -1.1761832162233077 1104 1 -0.47122658921258775 1104 2 -0.4446676887353682 1104 3 -0.5280934358000806 1104 4 -0.3818804214019451 1104 5 -0.3011700553301324 1104 6 -0.34514172804504284 1104 7 -0.4512520822985948 1104 8 -0.3810585553675736 1104 9 -0.4859159215961171 1104 10-11 -0.5025534625072368 1104 12-13 -0.49573936506564564 1104 14-15 -0.7082235949131643 1104 16-17 -0.822929228568249 1104 18-19 -0.7698331819033513 1104 20-21 -0.6958653726612454 1104 22-23 -0.6346091403027714 1104 24-25 -0.7055513006029273 1104 26-27 -0.7807990517736911 1104 28-29 -0.8361376994447696 1104 30-31 -0.8201235595516749 1104 32-33 -0.9176685811183489 1104 34-35 -0.9810487528586265 1104 36-37 -1.0670336191397638 1104 38-39 -0.9081205324858104 1104 40-41 -0.8797981676349238 1104 42-43 -0.7979606460205062 1104 44-45 -0.9640050713353716 1104 46-47 -1.0119358396048312 1104 48-49 -1.025288794480005 1104 50-51 -1.058516165363649 1104 52-53 -0.9017185333710813 1104 54-55 -0.6867728451346125 1104 56-57 -0.783917828192962 1104 58-59 -0.6816002330441293 1104 60-61 -0.6982159016043639 1104 62-63 -0.6910804949079932 1104 64-65 -0.6365960705437672 1104 66-67 -0.7979752687815491 1104 68-69 -0.895264729445941 1104 70-71 -0.7932029324697041 1104 72-73 -0.7240972529312444 1104 74-75 -0.8271948873205588 1104 76-77 -0.8098541155967922 1104 78-79 -0.8733371410163571 1104 80-81 -0.811445701408676 1104 82-83 -0.8554237428320803 1104 84-85 -0.875879075920345 1104 86-87 -0.8532973733164759 1104 88-89 -0.8679466877011706 1104 90-91 -0.9981747856065937 1104 92-93 -1.0035103305025146 1104 94-95 -0.9963622945045536 1104 96-97 -1.1440936685592753 1104 98-99 -0.9939474773916182 1104 100 -1.0687526758056691 1105 1 -0.5856675385773222 1105 2 -0.46868528762991346 1105 3 -0.5054715323787171 1105 4 -0.3390499718669844 1105 5 -0.3481900588204425 1105 6 -0.3803085477704471 1105 7 -0.30659898207229475 1105 8 -0.43050779422218 1105 9 -0.5819155096249204 1105 10-11 -0.5996511933756068 1105 12-13 -0.6903465476690158 1105 14-15 -0.8300921489228799 1105 16-17 -0.7789070128784417 1105 18-19 -0.7552025362529449 1105 20-21 -0.7965962818015697 1105 22-23 -0.8148273527196892 1105 24-25 -0.8365468762039399 1105 26-27 -0.7978005523215543 1105 28-29 -0.9022615261136124 1105 30-31 -0.8614039495796817 1105 32-33 -0.9047051314031549 1105 34-35 -0.8982820766079058 1105 36-37 -0.84194437659459 1105 38-39 -0.9291020967747841 1105 40-41 -0.9014744863003727 1105 42-43 -0.9379790702879021 1105 44-45 -1.0715969816801376 1105 46-47 -1.1708034302027386 1105 48-49 -0.9899028110847325 1105 50-51 -1.0310566043418419 1105 52-53 -0.8864430204812521 1105 54-55 -0.7759508231284826 1105 56-57 -0.7274658299896117 1105 58-59 -0.7003822216061977 1105 60-61 -0.6031478798671586 1105 62-63 -0.738801636182643 1105 64-65 -0.7965438517641772 1105 66-67 -0.7601908048161476 1105 68-69 -0.8842974625601343 1105 70-71 -0.7966367410003627 1105 72-73 -0.6723304078867756 1105 74-75 -0.7646974442598449 1105 76-77 -0.7001956797713191 1105 78-79 -0.8130882489041724 1105 80-81 -0.8209636307384507 1105 82-83 -0.84748559072316 1105 84-85 -0.8405796887274981 1105 86-87 -1.0343696570959686 1105 88-89 -0.966288914891841 1105 90-91 -0.9615617038045627 1105 92-93 -0.9167489998641578 1105 94-95 -1.0312787845099614 1105 96-97 -1.071313234902373 1105 98-99 -1.0656635627211521 1105 100 -1.062976961221601 1106 1 -0.4840245461266228 1106 2 -0.4684700823012591 1106 3 -0.560392337376566 1106 4 -0.47426953578457187 1106 5 -0.44880654769765727 1106 6 -0.4602354296470992 1106 7 -0.43784391800662803 1106 8 -0.38844426778845076 1106 9 -0.5624734090085752 1106 10-11 -0.5689150039558015 1106 12-13 -0.6213403059461484 1106 14-15 -0.7530935542884052 1106 16-17 -0.8088307864665083 1106 18-19 -0.8261836588131075 1106 20-21 -0.8170284596431685 1106 22-23 -0.8405346106084579 1106 24-25 -0.8050871540360447 1106 26-27 -0.834651234328966 1106 28-29 -0.7879857852931877 1106 30-31 -0.7943420646017216 1106 32-33 -0.8916548944747262 1106 34-35 -0.864589171328987 1106 36-37 -0.8082409240892403 1106 38-39 -0.8328045224971561 1106 40-41 -0.9227126538489969 1106 42-43 -0.9212714254346679 1106 44-45 -0.9180610414822112 1106 46-47 -0.8521213934564784 1106 48-49 -0.7909777550656898 1106 50-51 -0.866186342211833 1106 52-53 -0.9307213247666368 1106 54-55 -0.7393712607196363 1106 56-57 -0.7352569474088853 1106 58-59 -0.7386785022769757 1106 60-61 -0.7046950043160507 1106 62-63 -0.7445630760816115 1106 64-65 -0.7768424011363706 1106 66-67 -0.7911189397871254 1106 68-69 -0.8458816000903724 1106 70-71 -0.8013487667942698 1106 72-73 -0.7212372332694059 1106 74-75 -0.7347224013724443 1106 76-77 -0.6732350132424862 1106 78-79 -0.6510116746433141 1106 80-81 -0.7305555299097577 1106 82-83 -0.7831654258232099 1106 84-85 -0.6941857924283887 1106 86-87 -0.6695796516756722 1106 88-89 -0.7568771218354087 1106 90-91 -0.8436135374852789 1106 92-93 -0.7928520930653171 1106 94-95 -0.885000488606476 1106 96-97 -0.8407050441079384 1106 98-99 -0.804925892498698 1106 100 -0.9410776362440956 1107 1 -0.6310192360542644 1107 2 -0.6208106003084382 1107 3 -0.57854935674629 1107 4 -0.5145367381470649 1107 5 -0.46924323774188537 1107 6 -0.4236285974662266 1107 7 -0.4970441919204944 1107 8 -0.5236523695617592 1107 9 -0.6843032365421777 1107 10-11 -0.6218779567160979 1107 12-13 -0.7094419846424529 1107 14-15 -0.9090816079820954 1107 16-17 -0.8298199163100008 1107 18-19 -0.9275983262549872 1107 20-21 -0.8987033953419967 1107 22-23 -0.9618687275068538 1107 24-25 -0.8787789294272343 1107 26-27 -1.003477504794425 1107 28-29 -0.9843576300421333 1107 30-31 -0.8860476277884146 1107 32-33 -0.9732847181405901 1107 34-35 -1.0334107462474478 1107 36-37 -1.0531315081053734 1107 38-39 -1.0107763620149086 1107 40-41 -1.037142821048036 1107 42-43 -1.0821484130944938 1107 44-45 -1.0748501902748515 1107 46-47 -1.045094149127621 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1.160855781124539 2208 34-35 1.1106460895291832 2208 36-37 1.1545733327479368 2208 38-39 1.2476297117269723 2208 40-41 1.2054832776488311 2208 42-43 1.232062234761976 2208 44-45 1.207117635768249 2208 46-47 1.2327758561842117 2208 48-49 1.2202701524001611 2208 50-51 1.2575780989009147 2208 52-53 1.1872430487188268 2208 54-55 1.1157404735655234 2208 56-57 1.1127442069794213 2208 58-59 1.021245991165621 2208 60-61 1.028612335736618 2208 62-63 1.1276577148154274 2208 64-65 1.0752203131127587 2208 66-67 1.0727508334518774 2208 68-69 1.0708083350519004 2208 70-71 1.062472727544339 2208 72-73 1.0799860144274405 2208 74-75 1.0649017369787614 2208 76-77 1.005417651665013 2208 78-79 1.0014280540567384 2208 80-81 0.9954346039166921 2208 82-83 1.013736456665864 2208 84-85 0.9759398188187411 2208 86-87 1.0540783096070534 2208 88-89 1.030024442854156 2208 90-91 1.0398059472727788 2208 92-93 1.0516128478423816 2208 94-95 1.0932912274366586 2208 96-97 1.1693452520545797 2208 98-99 1.175359349838157 2208 100 1.2561034481252165 2209 1 0.7266778445985409 2209 2 0.7058257580590741 2209 3 0.7043024189654794 2209 4 0.6054848083603233 2209 5 0.5141279383496737 2209 6 0.5311924335614222 2209 7 0.5592210005832357 2209 8 0.6115074734760313 2209 9 0.7350021954277963 2209 10-11 0.7589334636146674 2209 12-13 0.8287072658322856 2209 14-15 0.9941496010428921 2209 16-17 0.9716307227553145 2209 18-19 0.9672725310709964 2209 20-21 1.0087083304582336 2209 22-23 1.0206572084195642 2209 24-25 1.0021615312998264 2209 26-27 1.0165936464095537 2209 28-29 1.0530647667340745 2209 30-31 1.0222554483846267 2209 32-33 1.0862908633935007 2209 34-35 1.146117770486896 2209 36-37 1.1637493451635947 2209 38-39 1.1553734612150919 2209 40-41 1.2111747103767954 2209 42-43 1.2365142886057043 2209 44-45 1.287220456359016 2209 46-47 1.2729585511747814 2209 48-49 1.2805183058078669 2209 50-51 1.3029003311641176 2209 52-53 1.1967539533651177 2209 54-55 1.1702650584726797 2209 56-57 1.0982713099985162 2209 58-59 1.1006533117362594 2209 60-61 1.120504785704064 2209 62-63 1.11201880290632 2209 64-65 1.1396838400519798 2209 66-67 1.130467999784699 2209 68-69 1.0870778854703573 2209 70-71 1.0980925939761264 2209 72-73 1.12171570330041 2209 74-75 1.1351710458583781 2209 76-77 1.0356080994974732 2209 78-79 1.1087652131433927 2209 80-81 1.144608166239962 2209 82-83 1.1075771118191717 2209 84-85 1.1683167693490866 2209 86-87 1.2155310686331866 2209 88-89 1.2451381812786764 2209 90-91 1.2648030321486985 2209 92-93 1.3350638966623833 2209 94-95 1.2987499707960772 2209 96-97 1.30097212727755 2209 98-99 1.3433786687818845 2209 100 1.4126626161210432 2210 1 0.8852122409116134 2210 2 0.8694944189445444 2210 3 0.8679537634220686 2210 4 0.6572602516269441 2210 5 0.6545086055188563 2210 6 0.7290369342936955 2210 7 0.7676511587852204 2210 8 0.8131620412783391 2210 9 0.9861634147760157 2210 10-11 0.9721199242045131 2210 12-13 0.9982279742439957 2210 14-15 1.2080198908044721 2210 16-17 1.204082163187934 2210 18-19 1.1824791285172154 2210 20-21 1.2024819990738536 2210 22-23 1.225540271593971 2210 24-25 1.226002009640709 2210 26-27 1.2457030160689087 2210 28-29 1.247482651775627 2210 30-31 1.2666929134405294 2210 32-33 1.300089340905913 2210 34-35 1.3497887968191833 2210 36-37 1.1675822178572801 2210 38-39 1.314543019612202 2210 40-41 1.3521091637322158 2210 42-43 1.4004667465199887 2210 44-45 1.3735700173366112 2210 46-47 1.4407129838410384 2210 48-49 1.4770153506970303 2210 50-51 1.4393930073333152 2210 52-53 1.396449557708479 2210 54-55 1.3114478837442505 2210 56-57 1.2409000570813191 2210 58-59 1.1578859766328051 2210 60-61 1.1448931649089573 2210 62-63 1.268602497847425 2210 64-65 1.240687966568295 2210 66-67 1.2363158596778163 2210 68-69 1.199735232955625 2210 70-71 1.145821029363212 2210 72-73 1.1608554163230451 2210 74-75 1.1050556822246804 2210 76-77 1.1893156827351774 2210 78-79 1.1467175058159356 2210 80-81 1.2249792584425876 2210 82-83 1.1865085070706236 2210 84-85 1.1487904019736952 2210 86-87 1.1350402327589713 2210 88-89 1.1253425604111413 2210 90-91 1.2260947585174335 2210 92-93 1.1876271808532728 2210 94-95 1.2683591288101859 2210 96-97 1.2618926822181677 2210 98-99 1.2905985896922054 2210 100 1.3666410939503528 2211 1 0.6356252481901521 2211 2 0.7234384819027575 2211 3 0.7060853572466819 2211 4 0.5830552208318593 2211 5 0.6040796534479824 2211 6 0.6243567607085652 2211 7 0.6573269221435183 2211 8 0.5732256149904345 2211 9 0.8150302208985991 2211 10-11 0.8940179599350557 2211 12-13 0.8867521607672444 2211 14-15 1.1224577308676018 2211 16-17 1.1370009574206605 2211 18-19 1.1039028531223494 2211 20-21 1.080773049564634 2211 22-23 1.1087680221334892 2211 24-25 1.1065300303455459 2211 26-27 1.1718174821921323 2211 28-29 1.136799586270726 2211 30-31 1.177803155122703 2211 32-33 1.1825785382817884 2211 34-35 1.1986810490380435 2211 36-37 1.2250581779955212 2211 38-39 1.2283467618407542 2211 40-41 1.2051860551162719 2211 42-43 1.2824475137562956 2211 44-45 1.2969862289829166 2211 46-47 1.3181813529904076 2211 48-49 1.3132677977418012 2211 50-51 1.2059358933057567 2211 52-53 1.1602828125564315 2211 54-55 1.1168544688451334 2211 56-57 1.0481035951422228 2211 58-59 0.9841269191656536 2211 60-61 0.9902349632258023 2211 62-63 1.025526215185053 2211 64-65 1.0171658460923325 2211 66-67 1.0926248774031961 2211 68-69 1.0553043860882596 2211 70-71 1.0805925341505258 2211 72-73 1.0462478445466203 2211 74-75 1.042781618238088 2211 76-77 1.0252768395162022 2211 78-79 1.0361919538764042 2211 80-81 0.9767455294255853 2211 82-83 0.9922503918106536 2211 84-85 1.0075169846309358 2211 86-87 1.0372611707520925 2211 88-89 1.05065967763203 2211 90-91 1.1164746609536635 2211 92-93 1.1258766940125362 2211 94-95 1.0991118027868332 2211 96-97 1.1282138601700247 2211 98-99 1.2054408526374623 2211 100 1.3225071327776163 2212 1 0.6239551382894497 2212 2 0.5964470455343971 2212 3 0.6538554479338963 2212 4 0.5060797157363552 2212 5 0.5389552706621288 2212 6 0.5155461888859989 2212 7 0.5737533184634316 2212 8 0.5429393166047802 2212 9 0.7570293524752145 2212 10-11 0.6594433358247684 2212 12-13 0.7552287516100691 2212 14-15 0.8617234224080619 2212 16-17 0.9437619998863909 2212 18-19 0.8851013450054666 2212 20-21 0.9089154413404827 2212 22-23 0.9702598130026558 2212 24-25 0.9638425173012095 2212 26-27 1.029611593007921 2212 28-29 1.0544181019646075 2212 30-31 1.016932901982301 2212 32-33 1.0329179147398406 2212 34-35 1.0876577313684592 2212 36-37 1.0838654130833163 2212 38-39 1.0819227274056473 2212 40-41 1.1414727512930511 2212 42-43 1.115429294094028 2212 44-45 1.1354973931958412 2212 46-47 1.219232588007543 2212 48-49 1.222428672193118 2212 50-51 1.220953195256918 2212 52-53 1.153988040609164 2212 54-55 1.0901552693779593 2212 56-57 1.0983950796424296 2212 58-59 1.0817202828581785 2212 60-61 0.9898710083358466 2212 62-63 1.0747502498818662 2212 64-65 1.0637658442167819 2212 66-67 1.0651047539483756 2212 68-69 1.0236483144631734 2212 70-71 0.9889041659861206 2212 72-73 1.0310502607787697 2212 74-75 1.0021775144032645 2212 76-77 1.0140528706817378 2212 78-79 1.0104927934468506 2212 80-81 0.8768887800289775 2212 82-83 0.9545279291957165 2212 84-85 0.9323890164908697 2212 86-87 0.8935018788726374 2212 88-89 0.9401225745589841 2212 90-91 1.0417573728946579 2212 92-93 1.0491564709354613 2212 94-95 1.105605295826905 2212 96-97 1.1260720690064439 2212 98-99 1.2693315595258134 2212 100 1.291638715931775 2213 1 0.6806503351460833 2213 2 0.6571540390619042 2213 3 0.6343029365509913 2213 4 0.5538529134362662 2213 5 0.5451574724748696 2213 6 0.5560437364633373 2213 7 0.5880940298981443 2213 8 0.6083968062996021 2213 9 0.6648759915521651 2213 10-11 0.7249861933998076 2213 12-13 0.8083222474536456 2213 14-15 1.021651170109898 2213 16-17 1.0116398218216176 2213 18-19 1.0167337528250684 2213 20-21 1.0154817197777959 2213 22-23 1.0373343029119724 2213 24-25 1.0851282161907179 2213 26-27 0.9601282204693007 2213 28-29 1.0516975798287262 2213 30-31 1.0861915470164192 2213 32-33 1.085993164735271 2213 34-35 1.1281357515815387 2213 36-37 1.157820457498353 2213 38-39 1.234818292036394 2213 40-41 1.2178670040504684 2213 42-43 1.234921962876527 2213 44-45 1.2204011019664804 2213 46-47 1.2713103457829291 2213 48-49 1.2557874502738144 2213 50-51 1.28865904193254 2213 52-53 1.191706809588993 2213 54-55 1.123156262868072 2213 56-57 1.1067427494995101 2213 58-59 1.0898290655082192 2213 60-61 1.0671659568304435 2213 62-63 1.0950444229541887 2213 64-65 1.0754860127802672 2213 66-67 1.1189036823908225 2213 68-69 1.0759697283661964 2213 70-71 1.0539678310759584 2213 72-73 1.0585984662675614 2213 74-75 1.0449180618039193 2213 76-77 1.019419797104014 2213 78-79 1.0621577034560588 2213 80-81 0.9352289078486535 2213 82-83 1.033037219242086 2213 84-85 1.0764980130070363 2213 86-87 1.1806312344780174 2213 88-89 1.174242688752571 2213 90-91 1.2630966853759773 2213 92-93 1.2973385458024715 2213 94-95 1.3551007776422956 2213 96-97 1.3621768314774307 2213 98-99 1.4852093970072993 2213 100 1.5390068705086684 2214 1 0.5816585260734257 2214 2 0.5988734740211044 2214 3 0.5990214921002739 2214 4 0.4709210784274731 2214 5 0.5010901864005959 2214 6 0.5848701083424004 2214 7 0.5687840680152192 2214 8 0.6025812018718781 2214 9 0.7521834927634785 2214 10-11 0.749597633978091 2214 12-13 0.8092408705259686 2214 14-15 0.9496944682512165 2214 16-17 0.91554366484214 2214 18-19 0.9341415255006851 2214 20-21 0.9573076711373787 2214 22-23 0.9787028838789382 2214 24-25 0.9761460499796968 2214 26-27 1.0195603252662409 2214 28-29 0.9443347278850283 2214 30-31 1.0442018494149465 2214 32-33 1.0757311296658543 2214 34-35 1.1025086512950395 2214 36-37 1.0745732842347167 2214 38-39 1.0000778268307613 2214 40-41 1.085539372050853 2214 42-43 1.2389618317875133 2214 44-45 1.2257697914731551 2214 46-47 1.2935979705060277 2214 48-49 1.3095663185702833 2214 50-51 1.192829920572585 2214 52-53 1.105952777823589 2214 54-55 1.078908707209422 2214 56-57 1.036622954855659 2214 58-59 0.9831271164522022 2214 60-61 1.0728972088142186 2214 62-63 1.1187645191757483 2214 64-65 1.0436784552449723 2214 66-67 0.9915752242240927 2214 68-69 1.004348885422658 2214 70-71 0.952259591893224 2214 72-73 1.039846629370615 2214 74-75 1.0409935009275628 2214 76-77 1.0456131875040953 2214 78-79 1.0078836894974117 2214 80-81 1.0417637850422636 2214 82-83 1.0504557575614122 2214 84-85 1.0728004426778703 2214 86-87 1.0993549176041917 2214 88-89 1.154128980466723 2214 90-91 1.1320120220203833 2214 92-93 1.2042650266238546 2214 94-95 1.2706358192476763 2214 96-97 1.3098039262588905 2214 98-99 1.4041673628071862 2214 100 1.5001432048458092 2215 1 0.5770848250205702 2215 2 0.5988174003622113 2215 3 0.589807236153856 2215 4 0.4494607239667374 2215 5 0.45691199851907527 2215 6 0.506205450299376 2215 7 0.5122409280256974 2215 8 0.5423347142682076 2215 9 0.6448784822269005 2215 10-11 0.655232518579588 2215 12-13 0.6495607435905839 2215 14-15 0.8300124614694013 2215 16-17 0.8522419985018033 2215 18-19 0.7921264604890865 2215 20-21 0.8146869834503576 2215 22-23 0.8487545506162206 2215 24-25 0.8785887054710813 2215 26-27 0.9880931834693243 2215 28-29 0.8772485387436433 2215 30-31 0.8624468663176899 2215 32-33 0.9132231688358559 2215 34-35 1.033745444005433 2215 36-37 1.1095271776477986 2215 38-39 1.134137775756102 2215 40-41 1.1292889223543128 2215 42-43 1.0802617090355042 2215 44-45 1.1986506563412362 2215 46-47 1.2116978895042365 2215 48-49 1.23721896197533 2215 50-51 1.2519410628264396 2215 52-53 1.1259120546421926 2215 54-55 1.000600129435469 2215 56-57 0.9689884493154324 2215 58-59 1.0322010737641918 2215 60-61 1.0110149644171358 2215 62-63 1.0025964651665333 2215 64-65 1.0392508925095356 2215 66-67 1.0498587368218182 2215 68-69 1.0358543010433792 2215 70-71 1.0480323984751223 2215 72-73 1.0337964194620923 2215 74-75 1.0344914217313317 2215 76-77 1.0071746003196225 2215 78-79 1.0861540665689446 2215 80-81 1.1576716627060932 2215 82-83 1.14174410252188 2215 84-85 1.1314519961146026 2215 86-87 1.1337246860300034 2215 88-89 1.1146968198583913 2215 90-91 1.1714457599615216 2215 92-93 1.1548283036587286 2215 94-95 1.0059765164479977 2215 96-97 1.250326289161091 2215 98-99 1.2670102634683111 2215 100 1.247290655921958 2216 1 0.4239737158296286 2216 2 0.36681627097157943 2216 3 0.38135008044243435 2216 4 0.27392220527908506 2216 5 0.2884873726727193 2216 6 0.3229527029163819 2216 7 0.304225922198782 2216 8 0.2545011693399033 2216 9 0.34196059759146635 2216 10-11 0.32080030664470627 2216 12-13 0.43010811926276205 2216 14-15 0.6121499335312066 2216 16-17 0.5029165588845714 2216 18-19 0.566159056620684 2216 20-21 0.5825879565468739 2216 22-23 0.593331827207912 2216 24-25 0.5383615907009016 2216 26-27 0.5124155299229969 2216 28-29 0.6492768311973833 2216 30-31 0.696165169265285 2216 32-33 0.719827435136736 2216 34-35 0.737934976822828 2216 36-37 0.6631999837908538 2216 38-39 0.47050253370410644 2216 40-41 0.6791881518411103 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1.528305220995417 2310 98-99 1.555715338228648 2310 100 1.6509179888503027 2311 1 0.751653737923057 2311 2 0.7078369435564973 2311 3 0.7537128385593839 2311 4 0.6457663743299591 2311 5 0.545881251295647 2311 6 0.6121357622111745 2311 7 0.6528403079640057 2311 8 0.5735098428178489 2311 9 0.8596626356845647 2311 10-11 0.8431704528975317 2311 12-13 0.9275818284683126 2311 14-15 1.0911837911963502 2311 16-17 1.1509031144130333 2311 18-19 1.0533602242687792 2311 20-21 1.0784062393763278 2311 22-23 1.0961221228753004 2311 24-25 1.1708256082207882 2311 26-27 1.1776269273969646 2311 28-29 1.2161960172506525 2311 30-31 1.1764341683890365 2311 32-33 1.2288060850371636 2311 34-35 1.2271871426113137 2311 36-37 1.2885778433865838 2311 38-39 1.26196284876778 2311 40-41 1.3332984396106156 2311 42-43 1.349809464515559 2311 44-45 1.4347953248054495 2311 46-47 1.388912349009317 2311 48-49 1.4015641222665352 2311 50-51 1.3975968625578972 2311 52-53 1.2566259813564074 2311 54-55 1.2378677529102333 2311 56-57 1.2402451645870514 2311 58-59 1.176589339507153 2311 60-61 1.2144126844284742 2311 62-63 1.1822518800201252 2311 64-65 1.1709178263318307 2311 66-67 1.1441178882052299 2311 68-69 1.1965600740809847 2311 70-71 1.138649664808426 2311 72-73 1.0932546734632353 2311 74-75 1.0653519148007078 2311 76-77 1.1083124552293597 2311 78-79 1.0248498757876874 2311 80-81 1.0587319230242684 2311 82-83 1.184889975141214 2311 84-85 1.1406677900946072 2311 86-87 1.08747158713334 2311 88-89 1.1545390959548243 2311 90-91 1.2478574168085856 2311 92-93 1.25003896347231 2311 94-95 1.296964010523876 2311 96-97 1.3033394028099643 2311 98-99 1.3660279235412283 2311 100 1.4505527998827858 2312 1 0.664109369369001 2312 2 0.6913483240300131 2312 3 0.6819417278537294 2312 4 0.417567056726476 2312 5 0.5009378341479405 2312 6 0.581188304627382 2312 7 0.4238138203669308 2312 8 0.557403672074166 2312 9 0.7796276696942144 2312 10-11 0.6917079322612523 2312 12-13 0.8570560781803067 2312 14-15 1.0072792747045085 2312 16-17 1.0642016932971714 2312 18-19 1.0646079997813018 2312 20-21 1.0252753641512342 2312 22-23 1.0839526228792664 2312 24-25 1.090962717740048 2312 26-27 1.1516672356829432 2312 28-29 1.1816889610309431 2312 30-31 1.1540900235397515 2312 32-33 1.1926704704087427 2312 34-35 1.204269655120882 2312 36-37 1.255797624071299 2312 38-39 1.3157608719156713 2312 40-41 1.325472805053252 2312 42-43 1.3381782265855193 2312 44-45 1.3564205382196306 2312 46-47 1.3753777474867448 2312 48-49 1.3711494178644443 2312 50-51 1.3531400561771036 2312 52-53 1.239616529873814 2312 54-55 1.1677006089538366 2312 56-57 1.1061014743749666 2312 58-59 1.0222352275437032 2312 60-61 1.0258734995141836 2312 62-63 1.0637751482252256 2312 64-65 1.1072861141970378 2312 66-67 1.0607914299594867 2312 68-69 1.0311274856369508 2312 70-71 1.0170195154339225 2312 72-73 1.0880193603691026 2312 74-75 1.1156943029769693 2312 76-77 1.038384022246916 2312 78-79 1.0175448528310582 2312 80-81 1.1102568262662231 2312 82-83 1.065830469495264 2312 84-85 1.062612871366074 2312 86-87 1.0464465071663689 2312 88-89 0.9880084546589956 2312 90-91 1.0690613768590396 2312 92-93 1.228177462993262 2312 94-95 1.2244958717070453 2312 96-97 1.1634597741352088 2312 98-99 0.8642979355550047 2312 100 1.1492924993709437 2313 1 0.5702879610607461 2313 2 0.633247169024461 2313 3 0.6221003369122435 2313 4 0.5116178782588321 2313 5 0.3751665989990087 2313 6 0.5545614153469884 2313 7 0.6121460629106394 2313 8 0.5606162621702282 2313 9 0.6819572455369141 2313 10-11 0.760808847643311 2313 12-13 0.8073444143798625 2313 14-15 0.9710694794249903 2313 16-17 1.029264241128665 2313 18-19 0.9836835759114138 2313 20-21 0.9760425448503867 2313 22-23 0.9911337482420066 2313 24-25 0.9582497095738702 2313 26-27 1.0274877004101697 2313 28-29 1.1238574460004145 2313 30-31 1.0846882654422103 2313 32-33 1.1305373197360709 2313 34-35 1.1371105330918638 2313 36-37 1.1819301882256568 2313 38-39 1.2419418606896286 2313 40-41 1.2328301740008953 2313 42-43 1.3389365823955615 2313 44-45 1.3510571408940564 2313 46-47 1.363619504201452 2313 48-49 1.267104229348206 2313 50-51 1.2873732584688113 2313 52-53 1.1549382659273562 2313 54-55 1.0675042456385313 2313 56-57 1.0236680071756012 2313 58-59 1.0454434996719328 2313 60-61 1.0000461672184215 2313 62-63 1.0323468075111322 2313 64-65 1.0166265835879358 2313 66-67 0.9450229503112126 2313 68-69 0.9996028252617322 2313 70-71 0.9379197634289866 2313 72-73 0.9339299330184048 2313 74-75 0.9443605423659207 2313 76-77 0.780587256011664 2313 78-79 0.9042353524884064 2313 80-81 0.982720316729754 2313 82-83 1.0453473002492295 2313 84-85 1.049209313719853 2313 86-87 1.1233761117503285 2313 88-89 1.1129965285606431 2313 90-91 1.1776316194176637 2313 92-93 1.1578660168536352 2313 94-95 1.2595262118707211 2313 96-97 1.272218313475829 2313 98-99 1.369835902998748 2313 100 1.3722941428423745 2314 1 0.5425813651635352 2314 2 0.5188002274248085 2314 3 0.5184364577522764 2314 4 0.397256971071954 2314 5 0.36755913889532366 2314 6 0.415346342796056 2314 7 0.47039122802889466 2314 8 0.45447252014923833 2314 9 0.671712782092257 2314 10-11 0.6080800659915013 2314 12-13 0.7057849563921152 2314 14-15 0.8815239487152766 2314 16-17 0.8251483308370169 2314 18-19 0.7757995092778955 2314 20-21 0.8569039571198545 2314 22-23 0.90023628215706 2314 24-25 0.8552932623811742 2314 26-27 0.9016864080206872 2314 28-29 0.8576996932515328 2314 30-31 0.9394684209484296 2314 32-33 0.8983835159274491 2314 34-35 0.9061773489758167 2314 36-37 0.9666665358607602 2314 38-39 0.9708606485873617 2314 40-41 1.0277771792010881 2314 42-43 1.08777035191779 2314 44-45 1.1410209423439142 2314 46-47 1.095801221121647 2314 48-49 1.0825712897262108 2314 50-51 1.1681302343482471 2314 52-53 1.1503294183559376 2314 54-55 1.0488959491339642 2314 56-57 0.9474753750295548 2314 58-59 1.0058491644373717 2314 60-61 0.9974930789613907 2314 62-63 1.0241502566950373 2314 64-65 1.1073986991255538 2314 66-67 1.0347170821563765 2314 68-69 1.0397771846165327 2314 70-71 1.0127365686439376 2314 72-73 1.042529636006357 2314 74-75 0.9875638775154627 2314 76-77 0.9579581150705465 2314 78-79 0.9883731003444254 2314 80-81 0.9474173639443464 2314 82-83 1.0260928669027614 2314 84-85 1.0879113701594143 2314 86-87 1.1573291864602062 2314 88-89 1.1202498539840207 2314 90-91 1.2070011544439438 2314 92-93 1.2018376035391078 2314 94-95 1.2167703445760232 2314 96-97 1.24586580329726 2314 98-99 1.4284347975402838 2314 100 1.4145507686293577 2315 1 0.48193284131032854 2315 2 0.5362081321884808 2315 3 0.5469262957104206 2315 4 0.2460782295798225 2315 5 0.354621928698144 2315 6 0.4832236735513291 2315 7 0.4708542817496806 2315 8 0.4976154576246188 2315 9 0.6302634417064894 2315 10-11 0.4910266566857544 2315 12-13 0.5162419293558642 2315 14-15 0.7641987150556488 2315 16-17 0.7638628248716728 2315 18-19 0.6591893111581513 2315 20-21 0.6983990945658434 2315 22-23 0.5595513815482605 2315 24-25 0.7860297713407292 2315 26-27 0.8216708965478219 2315 28-29 0.844263663777042 2315 30-31 0.8698773443567944 2315 32-33 0.9174315531731096 2315 34-35 0.9246417156109885 2315 36-37 0.9934304407466712 2315 38-39 1.0026773875904809 2315 40-41 1.050643863272839 2315 42-43 1.134167465098436 2315 44-45 1.076601357002673 2315 46-47 1.0899017415656118 2315 48-49 1.0446741305218978 2315 50-51 0.9745796277086782 2315 52-53 1.0467588889540451 2315 54-55 0.9331042249530412 2315 56-57 0.8952368707010265 2315 58-59 0.9127514207846659 2315 60-61 0.8754047795590978 2315 62-63 0.9177071835493962 2315 64-65 0.9368796410751727 2315 66-67 0.9462632571740244 2315 68-69 0.9676378427431942 2315 70-71 0.8590677421418533 2315 72-73 0.6182436962611533 2315 74-75 0.805173288119299 2315 76-77 0.9101825275248174 2315 78-79 0.8214244430869186 2315 80-81 0.5751668487580375 2315 82-83 0.64592392486691 2315 84-85 0.9241664554979359 2315 86-87 1.0749838000730811 2315 88-89 1.0737241317815887 2315 90-91 1.160011490506058 2315 92-93 1.2139533695414855 2315 94-95 1.2031224776699467 2315 96-97 1.0201658808484986 2315 98-99 0.5606837914655891 2315 100 1.002613560943118 2316 1 0.33004771631703633 2316 2 0.3113137970333497 2316 3 0.3630669979135419 2316 4 0.20626323482682096 2316 5 0.14905585447024805 2316 6 0.18292668300209414 2316 7 0.1608645659882626 2316 8 0.26288428219228877 2316 9 0.28720641322834695 2316 10-11 0.2479391826662649 2316 12-13 0.05964376053024978 2316 14-15 0.11901324402580116 2316 16-17 0.3067823702922894 2316 18-19 0.32657889748745106 2316 20-21 0.3220541080241972 2316 22-23 0.36213456388775 2316 24-25 0.41478547718065073 2316 26-27 0.4594421842122216 2316 28-29 0.45870703476166597 2316 30-31 0.3778215947764636 2316 32-33 0.48879692002800823 2316 34-35 0.49108480650897945 2316 36-37 0.5451581249668678 2316 38-39 0.6517302135351457 2316 40-41 0.6939659252780999 2316 42-43 0.6881952529013446 2316 44-45 0.5886826536927785 2316 46-47 0.35866489376185484 2316 48-49 0.3110791567815596 2316 50-51 0.32158091154193613 2316 52-53 0.47075179548222224 2316 54-55 0.18745630249878786 2316 56-57 0.036279835621726875 2316 58-59 0.04215913007693928 2316 60-61 0.1504487204645173 2316 62-63 0.3554812782495276 2316 64-65 0.3085957640097732 2316 66-67 0.21296465705199807 2316 68-69 0.22800385552476854 2316 70-71 -0.09236804120927644 2316 72-73 -0.26487000966145047 2316 74-75 0.05034274979154674 2316 76-77 0.22856420048109527 2316 78-79 0.2984517243895297 2316 80-81 0.4378922521845965 2316 82-83 0.4417130319642695 2316 84-85 0.5708294452562619 2316 86-87 0.6275119521737018 2316 88-89 0.5270079934237231 2316 90-91 0.40879107774225076 2316 92-93 0.5029405663739155 2316 94-95 0.6457511093109751 2316 96-97 0.49817482264538526 2316 98-99 0.48652341903672536 2316 100 0.31758801691800187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 17.0 1 344.0 2 137.0 3 109.0 4 105.0 5 84.0 6 100.0 7 430.0 8 1426.0 9 2742.0 10 4309.0 11 6607.0 12 9810.0 13 12669.0 14 14814.0 15 17555.0 16 21194.0 17 25778.0 18 31759.0 19 37716.0 20 44064.0 21 52629.0 22 58130.0 23 60251.0 24 60873.0 25 62603.0 26 67620.0 27 77415.0 28 90054.0 29 107677.0 30 130015.0 31 161173.0 32 203551.0 33 266188.0 34 373492.0 35 520503.0 36 777308.0 37 1143283.0 38 1048360.0 39 270842.0 40 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.61691997586451 18.31184562176304 12.317412892488074 23.75382150988437 2 16.231251889142513 19.52539528678486 40.77676328653471 23.466589537537914 3 19.5719856848498 25.496202147272527 29.137906951523696 25.793905216353973 4 12.871018263352216 15.801545897139851 37.60545925943663 33.7219765800713 5 13.328990666621321 36.90712014564707 34.21493285061944 15.548956337112172 6 30.89581977617828 35.809791979070674 18.612772588860153 14.681615655890894 7 27.704404786537477 31.916510554740086 22.333766836133165 18.04531782258927 8 26.265448122029422 35.1491911806695 20.244809373719363 18.340551323581714 9 26.870952967163113 16.077644715186253 20.838740369083666 36.21266194856697 10-11 25.702378783964576 25.735831694458906 27.845065903969235 20.716723617607286 12-13 25.69031539535127 23.512121321778203 28.0858819648863 22.711681317984226 14-15 23.063405851483694 24.910303058276597 26.148552051815916 25.877739038423798 16-17 22.38941923489798 27.39649750397423 26.998809573696203 23.215273687431583 18-19 21.97517351186523 26.84726574520892 29.06445747702378 22.11310326590207 20-21 23.068052050633298 26.351990924056473 28.72580200998555 21.85415501532468 22-23 22.941469226633867 26.12817339438365 28.568184791862393 22.362172587120085 24-25 22.43024395051925 25.949871640608972 29.051042857147813 22.56884155172396 26-27 22.36258644117853 26.188469141976356 28.61132082936334 22.83762358748178 28-29 22.149278821627647 26.757813720053274 28.67944643373981 22.41346102457927 30-31 22.99616529155063 26.343241109462706 28.729245291203597 21.931348307783068 32-33 22.87352922114064 26.418512649784905 28.371641208435715 22.33631692063874 34-35 22.90273626511971 26.549938697339947 28.518044308492023 22.029280729048317 36-37 22.718795946623874 26.434280829756222 28.355820372766644 22.491102850853256 38-39 22.680716483755983 26.683599049232313 28.380247034403727 22.255437432607973 40-41 22.745491747023838 26.046647505539493 28.681632197634826 22.52622854980184 42-43 22.577120596773263 26.37028732772246 28.20862594899886 22.843966126505418 44-45 22.401697946919892 26.81892511726407 28.374763224294153 22.404613711521886 46-47 22.36940981931484 26.40699215238299 27.975196437759685 23.24840159054249 48-49 22.699144376033363 26.476623656229364 27.98405572823927 22.840176239498 50-51 22.565582514857937 26.745475156800637 27.966934447203183 22.72200788113824 52-53 22.454474059868186 26.996116681780514 27.59623036104012 22.953178897311183 54-55 22.370619796748034 26.765286439348824 27.853414154190375 23.01067960971277 56-57 22.74634912902103 26.71955856063261 27.537234601527956 22.9968577088184 58-59 22.392758466098897 26.878060023982936 27.904728498018866 22.824453011899305 60-61 22.7000090755266 26.986017615545077 27.729421243430437 22.584552065497885 62-63 22.77990475665747 26.82902661685704 27.56757249214006 22.823496134345433 64-65 22.44788121026895 26.663621772818875 27.662285062105944 23.226211954806235 66-67 22.47925185636718 26.555732231914114 27.55733533486196 23.407680576856745 68-69 22.18243086281434 26.692910509185445 27.78681083771654 23.337847790283675 70-71 22.27703380165675 26.48702418827306 27.79884580385955 23.43709620621064 72-73 22.398553785924076 26.857025378345945 27.570369377834968 23.174051457895008 74-75 22.68020010530031 26.884611820498023 27.395582106998862 23.039605967202807 76-77 22.617162308613516 26.699021250275578 27.302066914591034 23.381749526519876 78-79 22.37144547496349 26.820657150886262 27.460719577209847 23.347177796940404 80-81 22.37454094844827 26.931625938341025 27.43925283595511 23.2545802772556 82-83 22.83039833043594 26.71092531478933 27.789480424189627 22.6691959305851 84-85 22.930061243671457 27.012810731774863 27.131829618464607 22.925298406089077 86-87 22.3955268391818 27.26997946041847 27.376533690769765 22.957960009629964 88-89 22.56638580863549 26.63911356721627 27.487491874967684 23.307008749180554 90-91 22.919841072767497 26.6442577672414 27.391886257049197 23.04401490294191 92-93 22.921974631434995 26.799548634651053 27.327194241708124 22.951282492205827 94-95 22.489858614941106 26.764927232080804 27.491973048391806 23.25324110458628 96-97 22.588313770328885 26.71777171330376 27.334938424321702 23.358976092045655 98-99 22.42149789419989 26.89904003211086 27.141042837646236 23.53841923604302 100 22.801201014010672 26.71388489368437 26.937365682458058 23.5475484098469 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1582.0 1 1214.5 2 2333.5 3 3501.5 4 2294.0 5 1545.5 6 2181.0 7 3757.5 8 5947.5 9 6874.0 10 5925.0 11 5011.0 12 5078.0 13 5446.5 14 5924.5 15 6614.0 16 7176.0 17 7782.5 18 8503.5 19 9027.0 20 9634.0 21 10555.5 22 12042.5 23 14065.0 24 16485.5 25 19757.0 26 23934.5 27 29053.5 28 35496.5 29 43655.5 30 54041.5 31 65122.0 32 77674.5 33 91001.0 34 102685.5 35 113480.0 36 125178.0 37 141619.5 38 152620.0 39 155217.0 40 156831.0 41 158670.5 42 163898.5 43 173076.5 44 183918.5 45 200907.5 46 217567.0 47 228833.0 48 239498.5 49 246977.5 50 248090.0 51 248430.5 52 244422.5 53 232922.0 54 221600.0 55 202471.0 56 181515.5 57 159048.5 58 136391.5 59 119615.5 60 98078.0 61 76085.5 62 59572.5 63 46828.5 64 34896.5 65 24082.0 66 17198.0 67 12830.0 68 9842.5 69 7527.0 70 5804.5 71 4345.0 72 3224.0 73 2413.5 74 1860.5 75 1341.0 76 933.5 77 633.0 78 423.0 79 310.5 80 218.5 81 141.5 82 103.0 83 82.5 84 76.0 85 63.0 86 50.5 87 45.5 88 34.5 89 27.5 90 19.5 91 10.5 92 12.0 93 12.0 94 8.0 95 5.5 96 5.0 97 4.5 98 4.5 99 4.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05686934104082344 2 0.1302894299013374 3 0.016741584534592624 4 0.016724235742328796 5 0.019395949750958087 6 0.051820842492049914 7 0.05666115553365753 8 0.04842047920833991 9 0.04002366375264786 10-11 0.0391995961201161 12-13 0.04027522124047334 14-15 0.01861525409908589 16-17 0.01784323284334561 18-19 0.01754830337486056 20-21 0.02017664540283028 22-23 0.024496494676523095 24-25 0.02366375264785942 26-27 0.018511161345502933 28-29 0.01773046569363074 30-31 0.01630786472799696 32-33 0.02228452366288521 34-35 0.02237126762420434 36-37 0.02236259322807243 38-39 0.020263389364149415 40-41 0.02395868211634447 42-43 0.030663990326313437 44-45 0.040041012544911686 46-47 0.046139113025646716 48-49 0.039407781627282014 50-51 0.027714695641462922 52-53 0.03929501447756714 54-55 0.03692690433355482 56-57 0.03836685409145243 58-59 0.0432505391137196 60-61 0.023230032841263758 62-63 0.025719584531122868 64-65 0.02493021448311876 66-67 0.03170491786214303 68-69 0.038696481144465125 70-71 0.040440034766979695 72-73 0.04448230336445128 74-75 0.026439559410071665 76-77 0.02041085409839194 78-79 0.01971690240783888 80-81 0.024166867623510387 82-83 0.0199250879150048 84-85 0.012473781637691292 86-87 0.014356125598316474 88-89 0.017973348785324313 90-91 0.02083589950885569 92-93 0.019621484050387834 94-95 0.02284835941145957 96-97 0.024019402889267863 98-99 0.026951348781854553 100 0.028209136220981975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5764090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.19485925242803 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9316920390711 53.70998796373805 2 12.780440033850146 17.175597385173145 3 3.5279490665828535 7.11180122936309 4 1.4006238394447157 3.7645888702833212 5 0.7155678870327078 2.4041241727360063 6 0.4008292989055678 1.6160200994525424 7 0.2635534354721498 1.2396605201431488 8 0.1858146432909961 0.9988631042382883 9 0.139135026940437 0.8414242697110903 >10 0.6196183557636151 7.0089950896986455 >50 0.023626377776829913 1.0789749881647226 >100 0.009931485812437699 1.2125081765424852 >500 8.224949388179369E-4 0.39031065996827685 >1k 2.7416431219845434E-4 0.4059280625928727 >5k 6.092540271072137E-5 0.31095018903967386 >10k+ 6.092540271072137E-5 0.7302652191547235 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20065 0.3481035167736798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15611 0.2708319960305963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9784 0.169740583509279 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.469758452765311E-5 2 0.0 0.0 0.0 0.0 3.469758452765311E-5 3 0.0 0.0 0.0 0.0 3.469758452765311E-5 4 0.0 0.0 0.0 0.0 5.204637679147966E-5 5 0.0 0.0 0.0 1.7348792263826555E-5 5.204637679147966E-5 6 0.0 0.0 0.0 1.7348792263826555E-5 6.939516905530622E-5 7 0.0 0.0 0.0 1.7348792263826555E-5 6.939516905530622E-5 8 0.0 0.0 0.0 1.7348792263826555E-5 6.939516905530622E-5 9 0.0 0.0 0.0 5.204637679147966E-5 6.939516905530622E-5 10-11 0.0 0.0 0.0 5.204637679147966E-5 6.939516905530622E-5 12-13 0.0 0.0 0.0 5.204637679147966E-5 6.939516905530622E-5 14-15 0.0 0.0 0.0 5.204637679147966E-5 7.80695651872195E-5 16-17 0.0 0.0 0.0 6.939516905530622E-5 8.674396131913277E-5 18-19 0.0 0.0 0.0 8.674396131913277E-5 8.674396131913277E-5 20-21 0.0 1.7348792263826555E-5 0.0 1.2144154584678588E-4 8.674396131913277E-5 22-23 0.0 1.7348792263826555E-5 0.0 3.12278260748878E-4 1.127671497148726E-4 24-25 0.0 1.7348792263826555E-5 0.0 8.240676325317613E-4 1.3879033811061244E-4 26-27 0.0 2.6023188395739833E-5 0.0 0.001665484057327349 1.3879033811061244E-4 28-29 0.0 5.204637679147966E-5 0.0 0.004345872462088552 1.3879033811061244E-4 30-31 0.0 5.204637679147966E-5 0.0 0.015466448303201373 1.3879033811061244E-4 32-33 0.0 5.204637679147966E-5 0.0 0.04404858355785562 1.56139130374439E-4 34-35 0.0 5.204637679147966E-5 0.0 0.08657914779262642 1.56139130374439E-4 36-37 0.0 6.072077292339294E-5 0.0 0.1544476231287159 1.7348792263826554E-4 38-39 0.0 7.80695651872195E-5 0.0 0.25903481729119426 1.7348792263826554E-4 40-41 0.0 8.674396131913277E-5 0.0 0.3790711109646102 1.7348792263826554E-4 42-43 0.0 8.674396131913277E-5 0.0 0.4911963553657212 1.7348792263826554E-4 44-45 0.0 8.674396131913277E-5 0.0 0.6232119900973094 1.7348792263826554E-4 46-47 0.0 8.674396131913277E-5 0.0 0.7718477678176434 1.7348792263826554E-4 48-49 0.0 8.674396131913277E-5 0.0 0.9313699126835285 1.908367149020921E-4 50-51 0.0 8.674396131913277E-5 0.0 1.1012059145502584 1.908367149020921E-4 52-53 0.0 9.541835745104604E-5 0.0 1.269202944437023 2.0818550716591864E-4 54-55 0.0 1.0409275358295932E-4 0.0 1.460646867068349 2.2553429942974519E-4 56-57 0.0 1.0409275358295932E-4 0.0 1.6744880805122753 2.2553429942974519E-4 58-59 0.0 1.0409275358295932E-4 0.0 1.9194790504659016 2.2553429942974519E-4 60-61 0.0 1.0409275358295932E-4 0.0 2.162240700613627 2.3420869556165847E-4 62-63 0.0 1.0409275358295932E-4 0.0 2.4076653903738494 2.4288309169357176E-4 64-65 0.0 1.0409275358295932E-4 0.0 2.6571670463160704 2.4288309169357176E-4 66-67 0.0 1.0409275358295932E-4 0.0 2.943508862630528 2.5155748782548505E-4 68-69 0.0 1.0409275358295932E-4 0.0 3.2674368373845653 2.602318839573983E-4 70-71 0.0 1.3011594197869915E-4 0.0 3.600585695226827 2.602318839573983E-4 72-73 0.0 1.56139130374439E-4 0.0 3.9122827714348665 3.12278260748878E-4 74-75 0.0 1.6481352650635228E-4 0.0 4.224456939430162 3.12278260748878E-4 76-77 0.0 1.7348792263826554E-4 0.0 4.570305113209544 3.12278260748878E-4 78-79 0.0 1.7348792263826554E-4 0.0 4.955101325621217 3.12278260748878E-4 80-81 0.0 1.7348792263826554E-4 0.0 5.357549934161334 3.12278260748878E-4 82-83 0.0 1.7348792263826554E-4 0.0 5.761733421927833 3.296270530127045E-4 84-85 0.0 1.7348792263826554E-4 0.0 6.162421128053171 3.296270530127045E-4 86-87 0.0 2.0818550716591864E-4 0.0 6.585983216778364 3.296270530127045E-4 88 0.0 2.0818550716591864E-4 0.0 6.927702377998956 3.296270530127045E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9180 0.0 49.21556 1 TACCTGG 5530 0.0 44.16847 2 GTATCAA 16240 0.0 39.58572 1 GTACCTG 6680 0.0 37.410362 1 ACCTGGG 6935 0.0 34.435055 3 CAACGCA 19275 0.0 32.21751 5 ATCAACG 19295 0.0 32.20848 3 TATCAAC 20010 0.0 31.656878 2 GTACATG 18440 0.0 30.192404 1 AACGCAG 21160 0.0 29.969786 6 TCAACGC 20860 0.0 29.926765 4 GTGGTAT 3980 0.0 29.798292 1 TACATGG 18800 0.0 28.98817 2 ACATGGG 18930 0.0 27.862865 3 CCTGGGG 6735 0.0 27.151136 4 TATCACG 490 0.0 26.892555 2 CATGGGG 15255 0.0 26.778336 4 GTACTAG 2725 0.0 26.769428 1 ACGCAGA 23205 0.0 26.639833 7 ATAACGC 660 0.0 26.353685 3 >>END_MODULE