FastQCFastQC Report
Mon 6 Jun 2016
ERR1211205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5764090
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186820.32411013707280767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155860.27039827622400064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99210.17211736804942324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94450.048.83051
TACCTGG56950.047.1983342
ACCTGGG67550.038.12833
GTATCAA160200.038.0335271
GTACCTG71200.037.96751
ATCAACG192000.031.0881373
CAACGCA193450.030.8230655
CCTGGGG66700.029.6652814
TATCAAC204050.029.593132
TCAACGC205600.029.1003024
AACGCAG210600.028.7815576
GTGGTAT37850.028.4442021
GTACATG187400.028.4239521
TACATGG188150.027.4984042
GTACTAG23850.027.41
TGGGGGG115850.026.4038876
ACATGGG189050.026.2778913
CTGGGGG46900.025.7479845
ACGCAGA234550.025.4419987
CATGGGG151400.025.207074
Produced by FastQC (version 0.11.5)