##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211205_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5764090 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15875706312705 33.0 31.0 34.0 30.0 34.0 2 32.2910176628054 34.0 31.0 34.0 30.0 34.0 3 32.369200342118184 34.0 31.0 34.0 30.0 34.0 4 35.988530713434386 37.0 35.0 37.0 35.0 37.0 5 35.872484988957495 37.0 35.0 37.0 35.0 37.0 6 35.89963897163299 37.0 35.0 37.0 35.0 37.0 7 35.83938939190748 37.0 35.0 37.0 35.0 37.0 8 35.8263953199898 37.0 35.0 37.0 35.0 37.0 9 37.43656466155109 39.0 37.0 39.0 34.0 39.0 10-11 37.41353596144404 39.0 37.0 39.0 34.0 39.0 12-13 37.312907674932205 39.0 37.0 39.0 34.0 39.0 14-15 38.54752137804927 40.0 38.0 41.0 34.0 41.0 16-17 38.49016783221636 40.0 38.0 41.0 34.0 41.0 18-19 38.51190508822728 40.0 38.0 41.0 34.0 41.0 20-21 38.47154026047477 40.0 38.0 41.0 34.0 41.0 22-23 38.342730335577684 40.0 38.0 41.0 34.0 41.0 24-25 38.28099448481894 40.0 38.0 41.0 34.0 41.0 26-27 38.14327664904607 40.0 38.0 41.0 33.5 41.0 28-29 38.11106705481698 40.0 37.5 41.0 33.5 41.0 30-31 38.11812966140362 40.0 37.5 41.0 33.5 41.0 32-33 37.97541381206747 40.0 37.0 41.0 33.0 41.0 34-35 37.82557862559398 40.0 37.0 41.0 33.0 41.0 36-37 37.67336604737261 40.0 37.0 41.0 33.0 41.0 38-39 37.584147801300816 40.0 37.0 41.0 33.0 41.0 40-41 37.486551042749156 40.0 36.5 41.0 32.0 41.0 42-43 37.34694721976929 40.0 36.0 41.0 32.0 41.0 44-45 37.222234559141164 40.0 36.0 41.0 31.0 41.0 46-47 36.996055665334865 39.0 36.0 41.0 31.0 41.0 48-49 36.777590825264696 39.0 35.5 41.0 30.5 41.0 50-51 36.58820785241035 39.0 35.0 41.0 30.0 41.0 52-53 36.392521369374876 39.0 35.0 40.5 30.0 41.0 54-55 36.158938878469975 38.5 35.0 40.0 29.0 41.0 56-57 35.91732354977108 38.0 35.0 40.0 28.5 41.0 58-59 35.65681122258674 38.0 34.5 40.0 28.0 41.0 60-61 35.38932450742442 37.5 34.0 40.0 28.0 41.0 62-63 35.08237145846092 37.0 34.0 40.0 27.5 41.0 64-65 34.70720608109866 36.5 34.0 39.0 27.0 41.0 66-67 34.39393399478495 36.0 33.0 39.0 26.0 40.5 68-69 34.05948024753257 35.5 33.0 39.0 26.0 40.0 70-71 33.713317193173594 35.0 33.0 38.0 26.0 40.0 72-73 33.360346732962185 35.0 32.5 37.5 26.0 39.5 74-75 33.01466328596535 35.0 32.0 37.0 25.0 39.0 76-77 32.01491024602322 34.0 30.5 36.0 24.5 38.0 78-79 32.50674885367855 35.0 32.0 36.0 25.0 38.0 80-81 32.43120735450002 35.0 32.0 35.5 25.0 37.0 82-83 32.178195864394894 35.0 32.0 35.0 25.0 37.0 84-85 31.920796517750418 34.0 31.5 35.0 25.0 36.5 86-87 31.68579021146443 34.0 31.0 35.0 24.0 36.0 88-89 31.475962120646972 34.0 31.0 35.0 24.0 36.0 90-91 31.26629875661206 34.0 31.0 35.0 23.0 35.0 92-93 31.088991584100874 34.0 31.0 35.0 23.0 35.0 94-95 30.901332040270017 34.0 30.5 35.0 21.0 35.0 96-97 30.731331571852625 34.0 30.0 35.0 20.0 35.0 98-99 30.557500229871497 34.0 30.0 35.0 20.0 35.0 100 28.784959291058954 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.40447126482850493 1101 2 -0.39063306554212573 1101 3 -0.4055326336439151 1101 4 -0.2543743913382457 1101 5 -0.3288772921744041 1101 6 -0.3834999353067161 1101 7 -0.3747746069194733 1101 8 -0.36706325106396065 1101 9 -0.4852457497225444 1101 10-11 -0.5314541764884737 1101 12-13 -0.5360433444764681 1101 14-15 -0.7240091173502563 1101 16-17 -0.7363657622476083 1101 18-19 -0.7303204994818628 1101 20-21 -0.7566687652427859 1101 22-23 -0.8619259450922954 1101 24-25 -0.8144837598461407 1101 26-27 -0.9827622294172542 1101 28-29 -1.021400697664383 1101 30-31 -0.8718469967522253 1101 32-33 -0.8564220843298784 1101 34-35 -0.7942280808151523 1101 36-37 -0.7390915294807385 1101 38-39 -0.8576887351255778 1101 40-41 -0.8662307140176821 1101 42-43 -0.8464207487526849 1101 44-45 -0.8390838456485383 1101 46-47 -0.9009277531711604 1101 48-49 -0.8836341122821381 1101 50-51 -0.9498002345982997 1101 52-53 -0.9466744376527956 1101 54-55 -0.7065096189261837 1101 56-57 -0.7703504421410088 1101 58-59 -0.849120668478399 1101 60-61 -0.9411027349731356 1101 62-63 -0.9851866396786733 1101 64-65 -0.8744654411014494 1101 66-67 -0.9690180657102871 1101 68-69 -1.063986934843662 1101 70-71 -1.1337973716024052 1101 72-73 -1.2688784494121776 1101 74-75 -1.2115226790838065 1101 76-77 -1.1660458387240382 1101 78-79 -0.9514935877801669 1101 80-81 -0.906167073011332 1101 82-83 -0.9556889275463156 1101 84-85 -0.8517931109866623 1101 86-87 -1.02126780390698 1101 88-89 -1.001951238217032 1101 90-91 -1.0443355192895858 1101 92-93 -1.0525278304843866 1101 94-95 -1.0641002931127233 1101 96-97 -1.077515197545985 1101 98-99 -1.293048105595549 1101 100 -1.0959054529885464 1102 1 -0.3820636710462253 1102 2 -0.3678769607058108 1102 3 -0.3914125031895743 1102 4 -0.2494174436197909 1102 5 -0.318854175135705 1102 6 -0.37395334440100925 1102 7 -0.4118458201116866 1102 8 -0.3607124006998177 1102 9 -0.4931948242835915 1102 10-11 -0.5452492611907829 1102 12-13 -0.47758948985997307 1102 14-15 -0.6486276309992789 1102 16-17 -0.622732913741153 1102 18-19 -0.633302765942652 1102 20-21 -0.7630913210395889 1102 22-23 -0.710818726146087 1102 24-25 -0.7887160268378182 1102 26-27 -0.8660446492979332 1102 28-29 -0.8243568585342942 1102 30-31 -0.776010792488151 1102 32-33 -0.6828525192300532 1102 34-35 -0.7219420500385851 1102 36-37 -0.7822369289861726 1102 38-39 -0.9042961337954694 1102 40-41 -0.7938044046219801 1102 42-43 -0.7078226770271456 1102 44-45 -0.8633801661900904 1102 46-47 -0.9324018724649292 1102 48-49 -0.9033418337184997 1102 50-51 -0.9354339839175196 1102 52-53 -1.0319040245742883 1102 54-55 -0.9802105826387049 1102 56-57 -1.094561017000565 1102 58-59 -1.074883789829336 1102 60-61 -1.1025212553042891 1102 62-63 -1.0218047212546821 1102 64-65 -1.0730323482160884 1102 66-67 -1.1576983296995351 1102 68-69 -1.1127030253587122 1102 70-71 -1.0817025076509026 1102 72-73 -1.1208922925954425 1102 74-75 -1.20213075389416 1102 76-77 -1.0520567104851324 1102 78-79 -0.897192100625066 1102 80-81 -0.9506111690351702 1102 82-83 -0.7900869650401177 1102 84-85 -0.917010546916476 1102 86-87 -0.8728709866088842 1102 88-89 -1.0037514657490547 1102 90-91 -0.9511081398761085 1102 92-93 -0.9615020839161623 1102 94-95 -1.0615654388219866 1102 96-97 -1.1061968751161686 1102 98-99 -1.1067442431714447 1102 100 -1.2034332351274806 1103 1 -0.3414020714241772 1103 2 -0.37944197196461715 1103 3 -0.37890848897925267 1103 4 -0.2462000175057355 1103 5 -0.2211260191845028 1103 6 -0.2990326301177575 1103 7 -0.3535845277215728 1103 8 -0.33401806935297174 1103 9 -0.559500668121494 1103 10-11 -0.5085909360514052 1103 12-13 -0.5614102164773058 1103 14-15 -0.711165135997895 1103 16-17 -0.7741540516164847 1103 18-19 -0.7308509295986028 1103 20-21 -0.8112790499217795 1103 22-23 -0.737098587415538 1103 24-25 -0.8250986490739081 1103 26-27 -0.6968794690953715 1103 28-29 -0.8417484885914277 1103 30-31 -0.8288393852319302 1103 32-33 -0.7603443356648967 1103 34-35 -0.8215341414648734 1103 36-37 -0.846131112042336 1103 38-39 -0.7977196157794424 1103 40-41 -0.8147351963605587 1103 42-43 -0.7279956027625403 1103 44-45 -0.8283984584507991 1103 46-47 -0.8865977339488111 1103 48-49 -0.9049378147112037 1103 50-51 -0.8665448869952712 1103 52-53 -0.888926710753168 1103 54-55 -0.8588114059013776 1103 56-57 -0.9140211725423626 1103 58-59 -0.9426922778863016 1103 60-61 -0.8744673191099963 1103 62-63 -0.9366973165185186 1103 64-65 -0.9228376797072713 1103 66-67 -0.9232546758288223 1103 68-69 -0.9508599444062398 1103 70-71 -1.022900921394175 1103 72-73 -0.9874950207017363 1103 74-75 -0.980216296601661 1103 76-77 -0.9423817046157055 1103 78-79 -0.7046904729283767 1103 80-81 -0.6750831335226088 1103 82-83 -0.737553939333182 1103 84-85 -0.7458496376435093 1103 86-87 -0.9342558963901446 1103 88-89 -0.8811556862857373 1103 90-91 -0.9416048249255518 1103 92-93 -1.058379833064695 1103 94-95 -0.9592219183579473 1103 96-97 -1.013429293438481 1103 98-99 -0.9732120607040251 1103 100 -0.9105864927643772 1104 1 -0.35596422470303324 1104 2 -0.3313895397607354 1104 3 -0.4053497999740898 1104 4 -0.25276568615885964 1104 5 -0.2921911728430544 1104 6 -0.42134162987691326 1104 7 -0.3848565675377529 1104 8 -0.3518093690172819 1104 9 -0.6256013043343742 1104 10-11 -0.48163317509297343 1104 12-13 -0.5493030467222866 1104 14-15 -0.7573202708613493 1104 16-17 -0.6610409444926546 1104 18-19 -0.7156276492136513 1104 20-21 -0.7955283771515056 1104 22-23 -0.6954800835755606 1104 24-25 -0.6837727445129573 1104 26-27 -0.8270492243279577 1104 28-29 -0.7795331667430005 1104 30-31 -0.7954925975711831 1104 32-33 -0.7721639838557905 1104 34-35 -0.8090302954462061 1104 36-37 -0.7670152467813907 1104 38-39 -0.8920597316893293 1104 40-41 -0.9992803247404964 1104 42-43 -0.7858742757777577 1104 44-45 -0.9617090121416538 1104 46-47 -0.958868548429372 1104 48-49 -0.921640855497003 1104 50-51 -0.8569871397930342 1104 52-53 -0.8920630352163457 1104 54-55 -0.8937202646919573 1104 56-57 -0.8667298046361509 1104 58-59 -0.8801464733401687 1104 60-61 -0.983994546119213 1104 62-63 -0.9831734470539573 1104 64-65 -0.91269405476217 1104 66-67 -1.01830055098862 1104 68-69 -1.0759129046493499 1104 70-71 -1.0888147817878462 1104 72-73 -0.9972467128960432 1104 74-75 -0.995356080379409 1104 76-77 -0.9048489285588879 1104 78-79 -0.8129690240784342 1104 80-81 -0.7603181303843414 1104 82-83 -0.7409797258884687 1104 84-85 -0.9387413003374867 1104 86-87 -0.9380639130562614 1104 88-89 -1.0037975404990434 1104 90-91 -1.0115349314195718 1104 92-93 -1.0206606585135596 1104 94-95 -1.060022159759633 1104 96-97 -1.1107564748668537 1104 98-99 -1.1785223478956972 1104 100 -1.1590106223243772 1105 1 -0.40954175825437034 1105 2 -0.40903109048898045 1105 3 -0.4269829367628333 1105 4 -0.23530643983727373 1105 5 -0.336808154066901 1105 6 -0.4248997092328679 1105 7 -0.45162987466514437 1105 8 -0.4565173558227613 1105 9 -0.630609829849412 1105 10-11 -0.5740092046633478 1105 12-13 -0.6100384546452986 1105 14-15 -0.8260577007332657 1105 16-17 -0.7818182754914815 1105 18-19 -0.7750329301694237 1105 20-21 -0.8904528169135943 1105 22-23 -0.7730904503729761 1105 24-25 -0.8245098117643934 1105 26-27 -0.863397063910945 1105 28-29 -0.8474212636363418 1105 30-31 -0.8278534893457845 1105 32-33 -0.724153908442176 1105 34-35 -0.8984820089888998 1105 36-37 -0.83235613148954 1105 38-39 -0.7636121371433688 1105 40-41 -0.9537579188001004 1105 42-43 -1.0457007007260515 1105 44-45 -0.9529846459049836 1105 46-47 -0.7963806178980164 1105 48-49 -0.9846169289823621 1105 50-51 -1.049489031570488 1105 52-53 -0.9550601185175012 1105 54-55 -1.0051580262865158 1105 56-57 -1.0560182316786282 1105 58-59 -1.0805986403372643 1105 60-61 -0.959597769221638 1105 62-63 -0.9677828897770837 1105 64-65 -1.0252182473415417 1105 66-67 -1.1409949606253136 1105 68-69 -1.0378043352193274 1105 70-71 -0.9828564516730438 1105 72-73 -0.9684496401802676 1105 74-75 -1.0085233342480748 1105 76-77 -1.0693949091218578 1105 78-79 -0.9556175375490312 1105 80-81 -0.892602993446836 1105 82-83 -0.8510127980206192 1105 84-85 -0.9099638248626469 1105 86-87 -0.8913717163342945 1105 88-89 -1.0322409867239308 1105 90-91 -0.9026506223725121 1105 92-93 -0.9378690923363919 1105 94-95 -0.9824132591791503 1105 96-97 -1.017782282430531 1105 98-99 -1.0948184550539857 1105 100 -1.045722119467758 1106 1 -0.3931100875178224 1106 2 -0.3515410230426106 1106 3 -0.37471409564457403 1106 4 -0.22462126127482662 1106 5 -0.2566820623241881 1106 6 -0.28377747037102097 1106 7 -0.3722190385424966 1106 8 -0.3756818757912441 1106 9 -0.5504228296203095 1106 10-11 -0.5491774014927557 1106 12-13 -0.5219275886272001 1106 14-15 -0.7088438588606394 1106 16-17 -0.7163295687071738 1106 18-19 -0.6910035963070982 1106 20-21 -0.6487424828668651 1106 22-23 -0.6219323509412646 1106 24-25 -0.7057667268333745 1106 26-27 -0.7305622907531486 1106 28-29 -0.7651871934407026 1106 30-31 -0.7286156486913171 1106 32-33 -0.7565223335387117 1106 34-35 -0.7255712678611488 1106 36-37 -0.6589160732632564 1106 38-39 -0.7129377083699779 1106 40-41 -0.6435755495717359 1106 42-43 -0.5774483761452842 1106 44-45 -0.6511978689138616 1106 46-47 -0.703545362780865 1106 48-49 -0.705046103309158 1106 50-51 -0.7994254338905904 1106 52-53 -0.7559658528559154 1106 54-55 -0.7691758839282983 1106 56-57 -0.7919251115436268 1106 58-59 -0.7230138574005807 1106 60-61 -0.8325958916756377 1106 62-63 -0.8862188827809945 1106 64-65 -0.8850174938085686 1106 66-67 -0.8108342586130988 1106 68-69 -0.8248784298342358 1106 70-71 -0.9373012694225551 1106 72-73 -0.902812922980047 1106 74-75 -0.8205296751567843 1106 76-77 -0.8048455341323333 1106 78-79 -0.5692885241547536 1106 80-81 -0.6781448764131355 1106 82-83 -0.7779530685884097 1106 84-85 -0.6287890175066906 1106 86-87 -0.7307317651169605 1106 88-89 -0.7451474041873425 1106 90-91 -0.7418211354007802 1106 92-93 -0.758661342709388 1106 94-95 -0.7460450953185607 1106 96-97 -0.7309267459049948 1106 98-99 -0.8198589675793393 1106 100 -0.6365218646579436 1107 1 -0.4245208621773422 1107 2 -0.44962383468328326 1107 3 -0.45036172287685616 1107 4 -0.3018220363252908 1107 5 -0.29352332403644965 1107 6 -0.3716899359143966 1107 7 -0.4099340893775363 1107 8 -0.42625298647662646 1107 9 -0.40974911114638246 1107 10-11 -0.4531984035962253 1107 12-13 -0.49433530234659173 1107 14-15 -0.6674264561756829 1107 16-17 -0.6596259345207542 1107 18-19 -0.7108422475734812 1107 20-21 -0.7089709772268478 1107 22-23 -0.696443029728151 1107 24-25 -0.7311137416689348 1107 26-27 -0.7356386292170782 1107 28-29 -0.8494700359398166 1107 30-31 -0.7901230709250626 1107 32-33 -0.8022427354886617 1107 34-35 -0.8100510450793479 1107 36-37 -0.773552809748189 1107 38-39 -0.8534740386170512 1107 40-41 -0.805164590846779 1107 42-43 -0.7776351963083314 1107 44-45 -0.9443157204652906 1107 46-47 -0.9456474179556977 1107 48-49 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2311 40-41 1.109701529454128 2311 42-43 1.1389915474923171 2311 44-45 1.0709438534899363 2311 46-47 0.8051816720038261 2311 48-49 1.0357404619906987 2311 50-51 1.1284083620918324 2311 52-53 1.0752281921812639 2311 54-55 1.1736492324647756 2311 56-57 1.1838292650718572 2311 58-59 1.2124754038561818 2311 60-61 1.303721893724891 2311 62-63 1.2900215564601893 2311 64-65 1.3163136763732126 2311 66-67 1.3064774184702017 2311 68-69 1.326224338977255 2311 70-71 1.3340144304030659 2311 72-73 1.3436228801012078 2311 74-75 1.324811960001398 2311 76-77 1.3303916311497517 2311 78-79 1.0262521119512158 2311 80-81 0.9952278411190747 2311 82-83 1.1240656386249768 2311 84-85 1.1453284076683872 2311 86-87 1.122623902418784 2311 88-89 1.1166404506836258 2311 90-91 1.2246145159000896 2311 92-93 1.3388147743661314 2311 94-95 1.3147893538816646 2311 96-97 1.4301242995245644 2311 98-99 1.3978353928237475 2311 100 1.5618346910741288 2312 1 0.2980929293291368 2312 2 0.4066852354247459 2312 3 0.4770047926266159 2312 4 0.22366165263257898 2312 5 0.40846441908144726 2312 6 0.4730860553694214 2312 7 0.3392643115800311 2312 8 0.4364412806981903 2312 9 0.6935600092439387 2312 10-11 0.7042963252158714 2312 12-13 0.7345975066547581 2312 14-15 0.8994231464251286 2312 16-17 0.922309365581107 2312 18-19 0.8783248954661218 2312 20-21 0.9432723330191592 2312 22-23 0.9773623049288815 2312 24-25 0.9875163777313318 2312 26-27 1.051400225690358 2312 28-29 1.0042643025158782 2312 30-31 1.0507632139735534 2312 32-33 0.9254745726481133 2312 34-35 1.0337973960113445 2312 36-37 1.0280259725193304 2312 38-39 0.8570962978609415 2312 40-41 0.9602052397978724 2312 42-43 1.0497131804842823 2312 44-45 0.7713999891089713 2312 46-47 0.8372306860898178 2312 48-49 0.9877803577714275 2312 50-51 0.8116385470757592 2312 52-53 0.9923070776414207 2312 54-55 0.9250302877201975 2312 56-57 0.9579885432229176 2312 58-59 1.0160826873944941 2312 60-61 1.0698789665593935 2312 62-63 1.1527247130949618 2312 64-65 1.1201701157300974 2312 66-67 1.1260153156277042 2312 68-69 1.204078624140024 2312 70-71 1.1017054851208314 2312 72-73 1.1187974235909834 2312 74-75 1.1170096170068078 2312 76-77 1.0645703358303678 2312 78-79 0.9814239847338868 2312 80-81 0.9353764135247644 2312 82-83 0.978068680410523 2312 84-85 1.056322223615247 2312 86-87 1.0916896881928189 2312 88-89 1.047213105816013 2312 90-91 1.078680887819413 2312 92-93 1.136922847217381 2312 94-95 1.2313796233410343 2312 96-97 0.8489558916097479 2312 98-99 0.6451121750359547 2312 100 0.8255229904683254 2313 1 0.05320297650141015 2313 2 0.2586337607743303 2313 3 0.3254773369264541 2313 4 0.1911611069553203 2313 5 0.32024649761694945 2313 6 0.2262261568179369 2313 7 0.26211232656886807 2313 8 0.3641726410749726 2313 9 0.560828762408697 2313 10-11 0.3555877585574976 2313 12-13 0.43901233643395443 2313 14-15 0.45975393106156304 2313 16-17 0.6660168129232531 2313 18-19 0.6732509104427606 2313 20-21 0.6574154485719106 2313 22-23 0.64220547186342 2313 24-25 0.5431890783117836 2313 26-27 0.7603915421350322 2313 28-29 0.5929685884887235 2313 30-31 0.8275179645854891 2313 32-33 0.8831659443722728 2313 34-35 0.9344212432257777 2313 36-37 0.9636221181399165 2313 38-39 0.915572267048141 2313 40-41 0.9323729884419834 2313 42-43 0.948947880846454 2313 44-45 0.8430659900606869 2313 46-47 0.8681300418517353 2313 48-49 1.0023858458271917 2313 50-51 0.8818867827757941 2313 52-53 0.9540724087997248 2313 54-55 0.9140439574437877 2313 56-57 0.6744428434308176 2313 58-59 0.7144465580357675 2313 60-61 0.5763735002968176 2313 62-63 0.9053085669117564 2313 64-65 1.1006445719339055 2313 66-67 0.9711140238977976 2313 68-69 1.0160360184396282 2313 70-71 1.011266682655318 2313 72-73 1.0307569107831611 2313 74-75 1.0997168591268505 2313 76-77 0.9991571056311699 2313 78-79 0.8901715596899464 2313 80-81 0.7705506379343632 2313 82-83 0.8090221887085534 2313 84-85 0.8456000129919659 2313 86-87 0.891759897536307 2313 88-89 0.8621622137862204 2313 90-91 0.7622361878029373 2313 92-93 0.9258563049267146 2313 94-95 1.0057968163094557 2313 96-97 1.0156375841717917 2313 98-99 1.0638528248053802 2313 100 1.2708668467864221 2314 1 -0.2600973440982486 2314 2 0.07429304944734838 2314 3 0.2890012270763265 2314 4 0.24958872871189897 2314 5 0.35127030836952144 2314 6 0.40575671534814006 2314 7 0.43012246163359436 2314 8 0.4716583214419714 2314 9 0.4378548407811067 2314 10-11 0.5912363873165916 2314 12-13 0.4800501413733187 2314 14-15 0.7784089272356098 2314 16-17 0.8155473619423148 2314 18-19 0.8363718262376238 2314 20-21 0.8753391274257325 2314 22-23 0.8836506614294208 2314 24-25 0.7856922264972468 2314 26-27 0.7580751570641056 2314 28-29 0.7942233014625302 2314 30-31 0.879098148575487 2314 32-33 0.8614956932485498 2314 34-35 0.9102316476954471 2314 36-37 0.859341028392187 2314 38-39 0.6248729861229734 2314 40-41 0.6710918733863664 2314 42-43 0.8742864792956269 2314 44-45 0.8653477761061481 2314 46-47 0.8680741949522215 2314 48-49 0.920159722247945 2314 50-51 0.9054583923541557 2314 52-53 0.8606714674290075 2314 54-55 0.8215779088850681 2314 56-57 0.7867756149455758 2314 58-59 0.9529787456165124 2314 60-61 0.977045561182635 2314 62-63 0.9714299416395278 2314 64-65 1.0436402293980933 2314 66-67 0.8200688784303694 2314 68-69 0.8426668450845725 2314 70-71 0.896365522363098 2314 72-73 1.0010954149480469 2314 74-75 1.05472048907194 2314 76-77 0.9261079964191978 2314 78-79 0.7551370131824839 2314 80-81 0.859205419901123 2314 82-83 0.7826507493050627 2314 84-85 0.7621257701361444 2314 86-87 0.8897946077179917 2314 88-89 0.9262614489917809 2314 90-91 1.0069097354858094 2314 92-93 0.8950992711534234 2314 94-95 0.8538707537172598 2314 96-97 0.8936187458402358 2314 98-99 1.006546069551181 2314 100 1.0761935492877335 2315 1 0.4449139186837314 2315 2 0.4636868716456206 2315 3 0.4612663363244849 2315 4 0.30664777476734173 2315 5 0.4040224832552539 2315 6 0.4244750235589265 2315 7 0.44021673878322076 2315 8 0.2898055933475945 2315 9 0.5489874275574422 2315 10-11 0.5741704294448624 2315 12-13 0.4600102836002051 2315 14-15 0.6825597772891925 2315 16-17 0.80476119887674 2315 18-19 0.8188030683331462 2315 20-21 0.49283923873377944 2315 22-23 0.8802851470567248 2315 24-25 0.9046008209421075 2315 26-27 0.9369864131032983 2315 28-29 0.8798456457245081 2315 30-31 0.8815625424022784 2315 32-33 0.7770922256798585 2315 34-35 0.858643782532333 2315 36-37 0.8437453778513202 2315 38-39 0.828109260677266 2315 40-41 0.7900910147067961 2315 42-43 0.9204704077120951 2315 44-45 0.9139796853885329 2315 46-47 0.8517891388122294 2315 48-49 0.7276263517282331 2315 50-51 0.858279030109145 2315 52-53 0.9278669253993073 2315 54-55 0.7764025201688938 2315 56-57 0.7439636619740995 2315 58-59 0.7279331981190253 2315 60-61 0.8907398436970979 2315 62-63 0.9974750445784935 2315 64-65 1.0213538169841172 2315 66-67 1.0438774521079424 2315 68-69 1.0541393638781713 2315 70-71 1.0015581177415385 2315 72-73 0.9995753481742398 2315 74-75 0.3627152116925956 2315 76-77 0.42343112211571565 2315 78-79 0.7280419094443076 2315 80-81 0.7895409443034893 2315 82-83 0.7089893502801985 2315 84-85 0.7729898335234893 2315 86-87 0.781358064113622 2315 88-89 0.8645806846328412 2315 90-91 0.9112069548546522 2315 92-93 0.9476655447602944 2315 94-95 0.9270553501044851 2315 96-97 1.0142436583018934 2315 98-99 1.0129139921584347 2315 100 0.9627142153183392 2316 1 0.3263133277872825 2316 2 0.12197944126781834 2316 3 0.269444557798991 2316 4 0.03960175727241477 2316 5 0.2997100126751491 2316 6 0.37396392713076665 2316 7 0.4375643059469496 2316 8 0.4449391064545978 2316 9 0.6497368135569346 2316 10-11 0.6037217294108714 2316 12-13 0.3707011105159168 2316 14-15 0.516627412660803 2316 16-17 0.8121703851016591 2316 18-19 0.8014302625251162 2316 20-21 0.7798368333200827 2316 22-23 0.8060829467108732 2316 24-25 0.5535795843287303 2316 26-27 0.8214091539176636 2316 28-29 0.9839863627339724 2316 30-31 0.5733023925004019 2316 32-33 0.7861157659133227 2316 34-35 0.8650340147883142 2316 36-37 0.8833055492840813 2316 38-39 0.8403449443323652 2316 40-41 0.8560150507676454 2316 42-43 0.8343125178132951 2316 44-45 0.6291494616955404 2316 46-47 0.8092493760715271 2316 48-49 0.9796067308569718 2316 50-51 0.9949843536678245 2316 52-53 0.9949105368636069 2316 54-55 0.8302831390986753 2316 56-57 1.031586762829356 2316 58-59 0.9046978210785994 2316 60-61 1.0119099519129477 2316 62-63 0.9906952821339132 2316 64-65 1.087958698018241 2316 66-67 1.0050207670135478 2316 68-69 1.0433902061431226 2316 70-71 1.033741430130064 2316 72-73 0.9820457185240414 2316 74-75 1.0020944372838727 2316 76-77 0.8748672756254052 2316 78-79 0.6816419173410182 2316 80-81 0.6689301148213502 2316 82-83 0.5508986404579801 2316 84-85 0.6309754183154013 2316 86-87 0.6530917078865919 2316 88-89 0.5894388984820722 2316 90-91 0.46839108467665724 2316 92-93 0.3198939054452623 2316 94-95 0.4115533256456416 2316 96-97 0.5790898234900865 2316 98-99 0.7692610392493897 2316 100 0.4597774390168752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 2.0 12 22.0 13 150.0 14 539.0 15 1536.0 16 3887.0 17 7955.0 18 13401.0 19 19001.0 20 25993.0 21 34416.0 22 42302.0 23 45881.0 24 47188.0 25 50613.0 26 58575.0 27 71225.0 28 87667.0 29 107824.0 30 132071.0 31 165495.0 32 210185.0 33 274409.0 34 391481.0 35 544947.0 36 844283.0 37 1297116.0 38 1121757.0 39 164143.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.613571773964836 19.117872182223312 13.198158966360765 24.070397077451087 2 15.92027536003081 19.911746693753916 40.44255034185795 23.72542760435732 3 19.135544379078052 25.385689675213257 29.7519990145886 25.72676693112009 4 12.784529692575001 16.169175727259663 37.48015554856762 33.566139031597714 5 13.160360091532228 37.14353870255322 34.17897708051054 15.517124125404012 6 31.057530330026072 36.07695230296543 18.27507898037678 14.590438386631716 7 27.749267701634476 32.00785767769989 22.084945916443385 18.157928704222243 8 26.375073949227023 35.516794498351 19.847452069624172 18.260679482797805 9 26.65272016619204 16.112736583433577 20.62825878861162 36.60628446176277 10-11 25.768519999597505 25.930636463912798 27.635986472049417 20.664857064440277 12-13 25.652856852140903 23.525573598880932 28.048196341173774 22.77337320780439 14-15 22.98350769185929 25.015123833270213 26.067130215553874 25.934238259316622 16-17 22.368218459317543 27.49957907386883 26.951104301114725 23.181098165698906 18-19 21.954124588616764 26.88011464081928 29.032058833224323 22.13370193733963 20-21 23.01269983860416 26.3612688839468 28.703165409139242 21.922865868309806 22-23 22.84090810518226 26.115492644979522 28.598443119382246 22.445156130455977 24-25 22.366977442982897 25.97161549324608 29.065641232048243 22.595765831722776 26-27 22.382472844885505 26.20600075137991 28.627348962185778 22.78417744154881 28-29 22.09625134995641 26.814718753659523 28.720731426985246 22.36829846939882 30-31 22.96143539722706 26.311229371031313 28.806045644207796 21.921289587533828 32-33 22.873790562002384 26.39741470644679 28.415368577325083 22.31342615422575 34-35 22.840807889255007 26.502671029533342 28.612154433818787 22.044366647392867 36-37 22.693857526348733 26.371152249730333 28.461071699142675 22.47391852477826 38-39 22.62484908614421 26.63010173426754 28.494175798861924 22.250873380726322 40-41 22.698114543094448 26.012115862314005 28.813080606674447 22.4766889879171 42-43 22.542217859578503 26.352767974262715 28.327737479417824 22.777276686740958 44-45 22.316557687255987 26.779248071562396 28.499981087795312 22.40421315338631 46-47 22.33440978562283 26.376510979310147 28.035584177660954 23.253495057406067 48-49 22.66327960872333 26.460221992015477 28.075542935244723 22.80095546401647 50-51 22.50649370591357 26.70103603465658 28.0140221191573 22.778448140272552 52-53 22.381203593984765 26.933943452578475 27.64879139080173 23.036061562635037 54-55 22.3372262089834 26.715401351951584 27.894151490755647 23.053220948309367 56-57 22.720099762875805 26.657761497608977 27.559858766412887 23.062279973102328 58-59 22.272688352933493 26.885116883948346 27.937141386336307 22.90505337678186 60-61 22.656302590990006 26.94676447217297 27.72465135968854 22.672281577148485 62-63 22.72731333978544 26.826343710918586 27.519958112490965 22.926384836805006 64-65 22.36355881866748 26.66550677905139 27.611662684434062 23.35927171784707 66-67 22.322329620468814 26.585045619445662 27.603099756251314 23.489525003834206 68-69 22.044448332868548 26.66103192352789 27.76056195366231 23.533957789941255 70-71 22.08407765546059 26.45928144632432 27.81033079623743 23.64631010197766 72-73 22.19024659609427 26.764510289105807 27.613515666432036 23.431727448367887 74-75 22.47531440122845 26.81619815399482 27.375259976286276 23.33322746849046 76-77 22.322684344513938 26.616001848562437 27.38014778954745 23.681166017376174 78-79 22.063790617414192 26.734010268547326 27.539504756881122 23.662694357157356 80-81 22.0395953838204 26.88140702933477 27.458147558379505 23.620850028465327 82-83 22.452811392569608 26.593897080683544 27.820708023056856 23.132583503689997 84-85 22.551465944399133 26.82318207298569 27.262610860691822 23.36274112192335 86-87 21.9741787397244 27.077258687623956 27.442555459571512 23.50600711308013 88-89 22.048231330669218 26.519063657443475 27.586000364053938 23.846704647833374 90-91 22.34211480730656 26.464906576441887 27.53948773322477 23.65349088302678 92-93 22.27410010236345 26.63443959219844 27.506802535725676 23.58465776971243 94-95 21.807403680151303 26.55570111133282 27.659416832224316 23.977478376291568 96-97 21.786830782939482 26.537935116981075 27.59376885123062 24.081465248848822 98-99 21.596560838182903 26.679741330580736 27.44862110854282 24.275076722693544 100 21.660794169419827 26.555928312436407 27.445263252340133 24.338014265803633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1587.0 1 1169.0 2 1956.5 3 2956.0 4 1959.5 5 1324.0 6 1959.5 7 3487.0 8 5365.0 9 6170.0 10 5391.0 11 4433.0 12 4432.0 13 4874.5 14 5467.5 15 6098.0 16 6810.0 17 7537.5 18 8242.0 19 8931.5 20 9670.0 21 10790.0 22 12181.5 23 14114.0 24 16644.0 25 19711.5 26 24046.5 27 29287.5 28 35695.5 29 44496.0 30 54992.0 31 65325.0 32 78201.5 33 91026.5 34 101974.0 35 113649.5 36 125941.5 37 141618.0 38 152585.5 39 155641.0 40 157117.0 41 158890.0 42 164700.0 43 174182.5 44 184780.0 45 202062.5 46 219026.0 47 231172.5 48 242742.5 49 249609.5 50 250049.0 51 250381.5 52 246702.0 53 235006.0 54 222945.0 55 203890.5 56 181861.0 57 158876.0 58 136139.5 59 118825.5 60 96584.5 61 73922.5 62 57600.0 63 44708.5 64 32605.0 65 22037.5 66 15555.0 67 11628.5 68 8863.0 69 6664.5 70 5023.5 71 3717.0 72 2760.5 73 2099.0 74 1592.5 75 1094.5 76 721.0 77 403.5 78 224.5 79 147.5 80 87.0 81 48.5 82 34.5 83 18.0 84 11.0 85 8.5 86 4.5 87 5.5 88 5.5 89 4.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044135327519174754 2 0.0 3 0.0 4 0.030776757476028305 5 0.0 6 0.0 7 7.980444441360215E-4 8 0.0 9 0.011051180672057515 10-11 4.1637101433183733E-4 12-13 1.7348792263826555E-5 14-15 1.56139130374439E-4 16-17 2.5155748782548505E-4 18-19 0.0 20-21 1.7348792263826555E-5 22-23 0.0 24-25 5.204637679147966E-4 26-27 4.944405795190567E-4 28-29 2.602318839573983E-4 30-31 0.0024028077285399778 32-33 0.015197542023112062 34-35 0.02633546665648871 36-37 0.03140998839365798 38-39 0.022249826078357553 40-41 0.0013185082120508182 42-43 1.7348792263826555E-5 44-45 0.010686856034517156 46-47 0.017747814485894565 48-49 0.0032789217378632186 50-51 0.010834320768759682 52-53 0.010869018353287336 54-55 0.009888811590381135 56-57 3.816734298041842E-4 58-59 0.008474885020879272 60-61 0.0046841739112331695 62-63 0.0013965777772380376 64-65 0.0353481642375466 66-67 0.059740566160486744 68-69 0.059011916885406024 70-71 0.05162133138101591 72-73 0.0448206048135959 74-75 0.0351486531265126 76-77 0.03078543187216022 78-79 0.014919961346890836 80-81 0.00103225313969768 82-83 0.03745604249760153 84-85 0.05160398258875208 86-87 0.05715559611317658 88-89 0.06860579900730211 90-91 0.0481515729282506 92-93 0.03934706085435862 94-95 0.01283810627523165 96-97 0.0036258975831397495 98-99 0.02238861641646817 100 0.03329233235428316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5764090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.14386936026648 #Duplication Level Percentage of deduplicated Percentage of total 1 78.76017970833475 52.09503037419204 2 13.507843210643184 17.869220333274903 3 3.7820414228199617 7.504765613583597 4 1.5096763963724324 3.9942335335174426 5 0.742885699641321 2.456866733334285 6 0.4185438347241496 1.661046523532347 7 0.2759015678344299 1.277443808139326 8 0.19043530494786987 1.0076902345643532 9 0.14044401765933354 0.8360559680840923 >10 0.6361045278323538 7.146364428644242 >50 0.024019366226085574 1.067130360238063 >100 0.010676636218380497 1.2558745368603585 >500 8.113998480069013E-4 0.3837134684941078 >1k 3.432839911017733E-4 0.5324174988824214 >5k 3.120763555465446E-5 0.20478866383934297 >10k+ 6.241527110930892E-5 0.7073579208191316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18682 0.32411013707280767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15586 0.27039827622400064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9921 0.17211736804942324 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7348792263826555E-5 0.0 5 0.0 1.7348792263826555E-5 0.0 1.7348792263826555E-5 0.0 6 0.0 1.7348792263826555E-5 0.0 1.7348792263826555E-5 0.0 7 0.0 1.7348792263826555E-5 0.0 1.7348792263826555E-5 0.0 8 0.0 1.7348792263826555E-5 0.0 1.7348792263826555E-5 0.0 9 0.0 1.7348792263826555E-5 0.0 3.469758452765311E-5 0.0 10-11 0.0 1.7348792263826555E-5 0.0 3.469758452765311E-5 0.0 12-13 0.0 1.7348792263826555E-5 0.0 3.469758452765311E-5 0.0 14-15 0.0 5.2046376791479666E-5 0.0 5.204637679147966E-5 8.674396131913278E-6 16-17 0.0 6.939516905530622E-5 0.0 6.939516905530622E-5 5.204637679147966E-5 18-19 0.0 6.939516905530622E-5 0.0 8.674396131913277E-5 6.939516905530622E-5 20-21 0.0 6.939516905530622E-5 0.0 1.3011594197869915E-4 6.939516905530622E-5 22-23 0.0 6.939516905530622E-5 0.0 3.2095265688079124E-4 7.80695651872195E-5 24-25 0.0 6.939516905530622E-5 0.0 8.500908209275011E-4 8.674396131913277E-5 26-27 0.0 6.939516905530622E-5 0.0 0.0016828328495911756 8.674396131913277E-5 28-29 0.0 8.674396131913277E-5 0.0 0.004389244442748118 8.674396131913277E-5 30-31 0.0 8.674396131913277E-5 0.0 0.015518494679992853 8.674396131913277E-5 32-33 0.0 8.674396131913277E-5 0.0 0.04396183959653649 1.56139130374439E-4 34-35 0.0 9.541835745104604E-5 0.0 0.08618879996669032 1.7348792263826554E-4 36-37 0.0 1.0409275358295932E-4 0.0 0.15406594969891169 2.0818550716591864E-4 38-39 0.0 1.0409275358295932E-4 0.0 0.2584883303348837 2.0818550716591864E-4 40-41 0.0 1.127671497148726E-4 0.0 0.378689437534806 2.0818550716591864E-4 42-43 0.0 1.2144154584678588E-4 0.0 0.4908580539165766 2.0818550716591864E-4 44-45 0.0 1.3011594197869915E-4 0.0 0.6231859669089136 2.168599032978319E-4 46-47 0.0 1.3879033811061244E-4 0.0 0.7725243707159326 2.6890628008931157E-4 48-49 0.0 1.3879033811061244E-4 0.0 0.932410840219358 3.12278260748878E-4 50-51 0.0 1.3879033811061244E-4 0.0 1.1022815396706158 3.12278260748878E-4 52-53 0.0 1.3879033811061244E-4 0.0 1.2699315937121036 3.2962705301270456E-4 54-55 0.0 1.474647342425257E-4 0.0 1.4612887723821106 3.469758452765311E-4 56-57 0.0 1.56139130374439E-4 0.0 1.674288569401241 3.469758452765311E-4 58-59 0.0 1.56139130374439E-4 0.0 1.9186029364565786 3.5565024140844434E-4 60-61 0.0 1.56139130374439E-4 0.0 2.1602889614839462 3.6432463754035765E-4 62-63 0.0 1.56139130374439E-4 0.0 2.4050457227420114 3.816734298041842E-4 64-65 0.0 1.7348792263826554E-4 0.0 2.6540702868969777 3.816734298041842E-4 66-67 0.0 2.2553429942974519E-4 0.0 2.93948394282532 3.903478259360975E-4 68-69 0.0 2.2553429942974519E-4 0.0 3.262292920478341 3.9902222206801075E-4 70-71 0.0 2.3420869556165847E-4 0.0 3.5950167329101386 4.163710143318373E-4 72-73 0.0 2.4288309169357176E-4 0.0 3.907086808151851 5.204637679147966E-4 74-75 0.0 2.4288309169357176E-4 0.0 4.218879302717341 5.204637679147966E-4 76-77 0.0 2.602318839573983E-4 0.0 4.565438776979541 5.291381640467099E-4 78-79 0.0 2.602318839573983E-4 0.0 4.950200291806686 5.378125601786231E-4 80-81 0.0 2.602318839573983E-4 0.0 5.352388668462845 5.464869563105365E-4 82-83 0.0 2.602318839573983E-4 0.0 5.755982297292374 5.551613524424498E-4 84-85 0.0 2.602318839573983E-4 0.0 6.155950028538763 5.551613524424498E-4 86-87 0.0 2.602318839573983E-4 0.0 6.578870211950195 5.551613524424498E-4 88 0.0 2.602318839573983E-4 0.0 6.920173002156455 5.551613524424498E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9445 0.0 48.8305 1 TACCTGG 5695 0.0 47.198334 2 ACCTGGG 6755 0.0 38.1283 3 GTATCAA 16020 0.0 38.033527 1 GTACCTG 7120 0.0 37.9675 1 ATCAACG 19200 0.0 31.088137 3 CAACGCA 19345 0.0 30.823065 5 CCTGGGG 6670 0.0 29.665281 4 TATCAAC 20405 0.0 29.59313 2 TCAACGC 20560 0.0 29.100302 4 AACGCAG 21060 0.0 28.781557 6 GTGGTAT 3785 0.0 28.444202 1 GTACATG 18740 0.0 28.423952 1 TACATGG 18815 0.0 27.498404 2 GTACTAG 2385 0.0 27.4 1 TGGGGGG 11585 0.0 26.403887 6 ACATGGG 18905 0.0 26.277891 3 CTGGGGG 4690 0.0 25.747984 5 ACGCAGA 23455 0.0 25.441998 7 CATGGGG 15140 0.0 25.20707 4 >>END_MODULE