FastQCFastQC Report
Mon 6 Jun 2016
ERR1211204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872733
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342570.3860929884850587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265950.29973853602942857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168230.18960336121914184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190600.044.071111
GTATCAA331950.038.559811
GTACTAG94900.038.529511
GCATCGT91350.037.1499068
GGGCACC94650.036.59523494
TACCTGG40250.033.670772
CAACGCA366700.033.02055
ATCAACG374900.032.4111023
TAGGCAT119950.032.4081275
CTGTGCG120950.032.061739
ACCTGGG41850.031.4493183
TAGTACT127850.031.0670324
TATCAAC413950.030.6704752
ACTGTGC132800.030.5451188
CTAGGCA131750.030.1830774
AACGCAG421200.029.4849556
GTACTGT137950.028.8273476
TCTAACG48000.028.6264862
CTAACGC47750.028.5478423
TAACGCC48850.028.193364
Produced by FastQC (version 0.11.5)