FastQCFastQC Report
Mon 6 Jun 2016
ERR1211204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872733
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309120.3483932177379845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257490.2902037061185094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166240.18736053479801545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196350.043.553251
GGGCACC87900.038.72207694
GTATCAA334750.037.6526031
GTACTAG95000.035.1360971
TAGGCAT115950.031.8522725
CAACGCA381200.031.7034635
ATCAACG387300.031.0912233
TACCTGG41500.030.2364042
CTGTGCG123750.030.1861769
TATCAAC427950.029.3983082
TAGTACT130250.028.8320274
ACCTGGG42250.028.701143
ACTGTGC134150.028.6165688
CTAGGCA133950.028.3865074
AACGCAG441050.028.2855996
CTAACGC44750.027.7279013
TATTCTC48050.027.6746235
GTGGTAT65250.027.523371
GCATCGT116450.027.2364468
TACTGTG152350.027.0486627
Produced by FastQC (version 0.11.5)