##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211204_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8872733 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.156565062872964 33.0 31.0 34.0 30.0 34.0 2 32.30589785582413 34.0 31.0 34.0 31.0 34.0 3 32.379917551897485 34.0 31.0 34.0 30.0 34.0 4 35.99195107076929 37.0 35.0 37.0 35.0 37.0 5 35.8842841320707 37.0 35.0 37.0 35.0 37.0 6 35.90786773365095 37.0 35.0 37.0 35.0 37.0 7 35.85044957399259 37.0 35.0 37.0 35.0 37.0 8 35.84233042964327 37.0 35.0 37.0 35.0 37.0 9 37.4407665597511 39.0 37.0 39.0 34.0 39.0 10-11 37.416605909362985 39.0 37.0 39.0 34.0 39.0 12-13 37.30323103377505 39.0 37.0 39.0 34.0 39.0 14-15 38.50116863654074 40.0 38.0 41.0 34.0 41.0 16-17 38.46681867920516 40.0 38.0 41.0 34.0 41.0 18-19 38.49976912412444 40.0 38.0 41.0 34.0 41.0 20-21 38.46272523922448 40.0 38.0 41.0 34.0 41.0 22-23 38.326607934669056 40.0 38.0 41.0 34.0 41.0 24-25 38.2521785564831 40.0 38.0 41.0 34.0 41.0 26-27 38.099897348426914 40.0 37.0 41.0 33.0 41.0 28-29 38.087282238742 40.0 37.0 41.0 33.0 41.0 30-31 38.0937526802621 40.0 37.0 41.0 33.5 41.0 32-33 37.954751371420734 40.0 37.0 41.0 33.0 41.0 34-35 37.816067044956725 40.0 37.0 41.0 33.0 41.0 36-37 37.67874853216027 40.0 36.5 41.0 33.0 41.0 38-39 37.574950412685695 40.0 36.0 41.0 33.0 41.0 40-41 37.46511176432335 40.0 36.0 41.0 32.0 41.0 42-43 37.305262200496735 39.5 36.0 41.0 32.0 41.0 44-45 37.116237465953276 39.0 36.0 41.0 31.0 41.0 46-47 36.83679402952845 39.0 35.0 41.0 31.0 41.0 48-49 36.54860464075725 39.0 35.0 41.0 30.0 41.0 50-51 36.31589996002359 39.0 35.0 40.0 30.0 41.0 52-53 36.09067679597707 38.0 35.0 40.0 29.0 41.0 54-55 35.834311648958675 38.0 35.0 40.0 29.0 41.0 56-57 35.541172545144775 38.0 34.0 40.0 28.0 41.0 58-59 35.23432627804759 37.0 34.0 40.0 28.0 41.0 60-61 34.96671871000739 37.0 34.0 40.0 27.5 41.0 62-63 34.65067854515627 36.0 34.0 39.0 27.0 41.0 64-65 34.27542533963323 35.5 33.0 39.0 26.0 41.0 66-67 33.98385074812913 35.0 33.0 39.0 26.0 40.0 68-69 33.68329493291413 35.0 33.0 38.0 26.0 40.0 70-71 33.41603308698684 35.0 33.0 37.5 26.0 40.0 72-73 33.1152469594205 35.0 32.0 37.0 25.0 39.5 74-75 32.8056907043185 35.0 32.0 36.5 25.0 39.0 76-77 31.84317706844103 34.0 30.5 35.5 23.5 38.0 78-79 32.36192101126 35.0 32.0 36.0 25.0 38.0 80-81 32.323076384694545 35.0 32.0 35.0 25.0 37.5 82-83 32.094808161138175 35.0 32.0 35.0 24.5 37.0 84-85 31.86039002864168 34.0 31.0 35.0 24.0 36.5 86-87 31.642374001336457 34.0 31.0 35.0 24.0 36.0 88-89 31.442492127284794 34.0 31.0 35.0 24.0 36.0 90-91 31.234144654189414 34.0 31.0 35.0 23.0 35.5 92-93 31.043365161557325 34.0 31.0 35.0 22.5 35.0 94-95 30.8675021551984 34.0 30.5 35.0 20.0 35.0 96-97 30.7078494867365 34.0 30.0 35.0 20.0 35.0 98-99 30.547542003123503 34.0 30.0 35.0 20.0 35.0 100 28.70617238228627 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3615655492692831 1101 2 -0.3390427042750055 1101 3 -0.3799854233447917 1101 4 -0.2230508022094213 1101 5 -0.28643509296227876 1101 6 -0.3674809307370026 1101 7 -0.3389144564406834 1101 8 -0.3685491652037456 1101 9 -0.5260156925323969 1101 10-11 -0.5011079846417914 1101 12-13 -0.4798669171212353 1101 14-15 -0.6734229744507871 1101 16-17 -0.6731276252419747 1101 18-19 -0.6989209413906465 1101 20-21 -0.7227515369006383 1101 22-23 -0.7895384822441898 1101 24-25 -0.7400981696630851 1101 26-27 -0.898940299006334 1101 28-29 -0.921613447849218 1101 30-31 -0.7090872855664969 1101 32-33 -0.6826594112839999 1101 34-35 -0.6997046680746735 1101 36-37 -0.6433519668563221 1101 38-39 -0.7639725149825622 1101 40-41 -0.7762835369341872 1101 42-43 -0.7757882523472475 1101 44-45 -0.8211053154257044 1101 46-47 -0.7637802774828515 1101 48-49 -0.768387963216675 1101 50-51 -0.7776756594031511 1101 52-53 -0.7868315407139121 1101 54-55 -0.7694663598123839 1101 56-57 -0.7978523683016334 1101 58-59 -0.8163605104783116 1101 60-61 -0.8499123471161241 1101 62-63 -0.9650517267945062 1101 64-65 -0.9151313527244227 1101 66-67 -0.9956581563981928 1101 68-69 -1.1006755367506287 1101 70-71 -1.047130287493573 1101 72-73 -1.0952921917208265 1101 74-75 -1.1144739202994494 1101 76-77 -1.0472928820994554 1101 78-79 -0.871782750331004 1101 80-81 -0.8680368600367814 1101 82-83 -0.8375508781353034 1101 84-85 -0.820489500185456 1101 86-87 -0.95968318660222 1101 88-89 -0.9203159241381442 1101 90-91 -1.0080146047975198 1101 92-93 -1.0423444576576806 1101 94-95 -0.9569108001686928 1101 96-97 -0.9203503104426964 1101 98-99 -1.2131888580482801 1101 100 -1.0149505534773482 1102 1 -0.46288553696529533 1102 2 -0.36907693261638386 1102 3 -0.40178704908806395 1102 4 -0.2614592266898015 1102 5 -0.3411453929894037 1102 6 -0.3947545556369505 1102 7 -0.3781790012356865 1102 8 -0.45444673677746295 1102 9 -0.6091831618075645 1102 10-11 -0.5491362328179079 1102 12-13 -0.5707054679539922 1102 14-15 -0.720549953561715 1102 16-17 -0.7320450314412525 1102 18-19 -0.7162212955274541 1102 20-21 -0.775238834512912 1102 22-23 -0.7050955754717378 1102 24-25 -0.790924765341984 1102 26-27 -0.8286012736557851 1102 28-29 -0.8648793146349334 1102 30-31 -0.8240329382359519 1102 32-33 -0.7469246208097999 1102 34-35 -0.7336548225598776 1102 36-37 -0.8147408374275287 1102 38-39 -0.8201411417128668 1102 40-41 -0.859947193919453 1102 42-43 -0.8178446444014682 1102 44-45 -0.8970863536563769 1102 46-47 -0.9731259894368876 1102 48-49 -0.8946323407265879 1102 50-51 -0.9509512330231686 1102 52-53 -1.0619725636451776 1102 54-55 -1.0498467481834055 1102 56-57 -1.0525549856879408 1102 58-59 -1.0676293668466244 1102 60-61 -1.0042224306763146 1102 62-63 -1.0756365910626542 1102 64-65 -1.0437150311246057 1102 66-67 -1.1284266239578287 1102 68-69 -1.1638385505647406 1102 70-71 -1.1386723867475226 1102 72-73 -1.0937308464515958 1102 74-75 -1.2275908500338168 1102 76-77 -1.071939305615441 1102 78-79 -0.9826861265339843 1102 80-81 -0.9033146230917808 1102 82-83 -0.9077470746045435 1102 84-85 -1.0182647712353194 1102 86-87 -0.9990590664513661 1102 88-89 -0.9811738368728733 1102 90-91 -0.9622495884848483 1102 92-93 -1.0201484862556924 1102 94-95 -1.1378957141328208 1102 96-97 -1.298555923205953 1102 98-99 -1.328093869489006 1102 100 -1.230952514844958 1103 1 -0.3788699009465901 1103 2 -0.34085925668660266 1103 3 -0.3747854780445934 1103 4 -0.24954678694365384 1103 5 -0.202287621273598 1103 6 -0.31711482643162725 1103 7 -0.3970860185592855 1103 8 -0.3299990210971302 1103 9 -0.5340506141558734 1103 10-11 -0.5522891282146389 1103 12-13 -0.5721859778065692 1103 14-15 -0.7271340584442783 1103 16-17 -0.7147114415911275 1103 18-19 -0.7169035007075735 1103 20-21 -0.7140648302452206 1103 22-23 -0.6905615366450704 1103 24-25 -0.8633612247831053 1103 26-27 -0.8244997497541533 1103 28-29 -0.7876001841929323 1103 30-31 -0.7979576621215827 1103 32-33 -0.7152048442075696 1103 34-35 -0.9078826600180179 1103 36-37 -0.8516338424287326 1103 38-39 -0.7991777241977971 1103 40-41 -0.8807060259345718 1103 42-43 -0.7710328698477866 1103 44-45 -0.9224488128197166 1103 46-47 -0.9234089340499736 1103 48-49 -0.9437817108407671 1103 50-51 -0.9601298354040821 1103 52-53 -0.9722278169643843 1103 54-55 -0.9777584117098215 1103 56-57 -1.008846320354131 1103 58-59 -0.9571455622139027 1103 60-61 -1.018582627406012 1103 62-63 -0.9226050282646199 1103 64-65 -0.9612804864914395 1103 66-67 -1.035822185019967 1103 68-69 -1.0939947457728891 1103 70-71 -1.1553960228022646 1103 72-73 -1.1370553070028677 1103 74-75 -1.0764334062515388 1103 76-77 -0.9768038304663449 1103 78-79 -0.9064443884944531 1103 80-81 -0.8033388316934094 1103 82-83 -0.8488383413836864 1103 84-85 -0.9215761107011069 1103 86-87 -0.9509854375515623 1103 88-89 -1.0188275436497243 1103 90-91 -0.9863996723374058 1103 92-93 -1.1646575009468876 1103 94-95 -1.0869044602360738 1103 96-97 -1.1897427051456582 1103 98-99 -1.1706169451772155 1103 100 -1.2022245744797893 1104 1 -0.37163139266827017 1104 2 -0.3419117608460205 1104 3 -0.429882744787772 1104 4 -0.2647959761298466 1104 5 -0.3167998785785642 1104 6 -0.36375951996564737 1104 7 -0.30917987544280123 1104 8 -0.3225573009321039 1104 9 -0.5258320030708887 1104 10-11 -0.5151372282241695 1104 12-13 -0.5567478129547041 1104 14-15 -0.7680791385201786 1104 16-17 -0.6529484507546996 1104 18-19 -0.6979271875015982 1104 20-21 -0.7467685212824549 1104 22-23 -0.7150648389602523 1104 24-25 -0.7091673316826501 1104 26-27 -0.7156386214059154 1104 28-29 -0.8404665944232264 1104 30-31 -0.7525070214590883 1104 32-33 -0.7471353864172272 1104 34-35 -0.7570702354903176 1104 36-37 -0.623302509758993 1104 38-39 -0.721797029424657 1104 40-41 -0.7858693650133688 1104 42-43 -0.6882930920239474 1104 44-45 -0.7430233736851761 1104 46-47 -0.8111139478355085 1104 48-49 -0.8311650673804962 1104 50-51 -0.859167694640881 1104 52-53 -0.807112383176154 1104 54-55 -0.8189493797956331 1104 56-57 -0.6886589033513033 1104 58-59 -0.7823744725133466 1104 60-61 -0.7900654103643276 1104 62-63 -0.8330878330246847 1104 64-65 -0.7767221168462513 1104 66-67 -0.820193791714992 1104 68-69 -0.9269215468663603 1104 70-71 -0.9061596536991914 1104 72-73 -0.8902665917217547 1104 74-75 -0.899644677954452 1104 76-77 -0.7895653091720547 1104 78-79 -0.717025040835523 1104 80-81 -0.8080319036486401 1104 82-83 -0.7350606730847069 1104 84-85 -0.6854523587295951 1104 86-87 -0.8093031490050002 1104 88-89 -0.824548194175069 1104 90-91 -0.8429446081963157 1104 92-93 -0.9341899216218472 1104 94-95 -0.8753509614935808 1104 96-97 -0.9719745671424995 1104 98-99 -0.9687217055417534 1104 100 -1.012450541542215 1105 1 -0.400688732229316 1105 2 -0.3889472234524405 1105 3 -0.4082804166956997 1105 4 -0.27502547335672034 1105 5 -0.3236639450786569 1105 6 -0.35801675078515416 1105 7 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-0.8165069387555093 1105 70-71 -0.898791372128926 1105 72-73 -0.9350684672882963 1105 74-75 -0.9528648845503085 1105 76-77 -0.8812265382536815 1105 78-79 -0.8346970230890562 1105 80-81 -0.8149267745415756 1105 82-83 -0.7314763380968969 1105 84-85 -0.7796938621790694 1105 86-87 -0.7622260888548951 1105 88-89 -0.913783664420162 1105 90-91 -0.8838616535196664 1105 92-93 -0.8532659759402996 1105 94-95 -0.8468112504141061 1105 96-97 -0.981897435817114 1105 98-99 -0.9322508431803236 1105 100 -1.0922117084939096 1106 1 -0.43069453959821047 1106 2 -0.4336435101707181 1106 3 -0.4501395748229946 1106 4 -0.30315866857571905 1106 5 -0.32605528354061875 1106 6 -0.3925437404406864 1106 7 -0.41504654054710954 1106 8 -0.4259423106142961 1106 9 -0.6217251614461929 1106 10-11 -0.5524295651328828 1106 12-13 -0.6360320065922451 1106 14-15 -0.7398089703283688 1106 16-17 -0.7314865448215073 1106 18-19 -0.7048515264056974 1106 20-21 -0.7515493342908357 1106 22-23 -0.7417568950772804 1106 24-25 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2310 80-81 1.0153470203187922 2310 82-83 1.0209279989958944 2310 84-85 1.0065610572724246 2310 86-87 1.0369245609827615 2310 88-89 1.0630216662591216 2310 90-91 1.1679530651414858 2310 92-93 1.1345094138690932 2310 94-95 1.1892254010150225 2310 96-97 1.2688557967190626 2310 98-99 1.2204588056317363 2310 100 1.3427604419052095 2311 1 0.4137005978973676 2311 2 0.3664556282690299 2311 3 0.41468537716352927 2311 4 0.3097254660551414 2311 5 0.37433805143786714 2311 6 0.4485622325430185 2311 7 0.4727029954120354 2311 8 0.47530507412653833 2311 9 0.6209584431588127 2311 10-11 0.6323436717800917 2311 12-13 0.6764270344257497 2311 14-15 0.8462311785938823 2311 16-17 0.6713290297844594 2311 18-19 0.7913161935174813 2311 20-21 0.6877277276063225 2311 22-23 0.865589005805738 2311 24-25 0.865949958201 2311 26-27 0.2345771946042916 2311 28-29 0.5650328265902189 2311 30-31 0.8169396773334014 2311 32-33 0.8972584819018721 2311 34-35 0.9810869798631572 2311 36-37 1.0110266367196843 2311 38-39 1.0289820929432523 2311 40-41 0.9875103747215093 2311 42-43 0.9304430442822849 2311 44-45 0.9498764468781928 2311 46-47 0.5371811417679879 2311 48-49 0.8276314278646026 2311 50-51 1.0085858703089414 2311 52-53 0.8512645518362945 2311 54-55 0.8389669902702721 2311 56-57 0.9653983654723248 2311 58-59 0.9938153694708873 2311 60-61 1.0522396450248337 2311 62-63 1.0306057403885092 2311 64-65 1.065015236479887 2311 66-67 1.099187069908858 2311 68-69 1.1142568301998637 2311 70-71 1.1727468653942452 2311 72-73 1.13496134528188 2311 74-75 1.0988706889721271 2311 76-77 1.0573587796211967 2311 78-79 0.7933280917605998 2311 80-81 0.7437290355889346 2311 82-83 0.8909861772612047 2311 84-85 0.940088854288252 2311 86-87 0.9641938314078686 2311 88-89 1.063023178629951 2311 90-91 1.0693186879922187 2311 92-93 1.1334819619378855 2311 94-95 1.1674962090171448 2311 96-97 1.1866966833044543 2311 98-99 1.25263492721675 2311 100 1.357714428622753 2312 1 0.2591378669636697 2312 2 0.34385017719584 2312 3 0.4487130401428061 2312 4 0.15943608891092964 2312 5 0.33212057841394227 2312 6 0.43366066360918865 2312 7 0.3057509805567946 2312 8 0.41447118409909933 2312 9 0.6298930627385886 2312 10-11 0.6477057918599627 2312 12-13 0.6967986230231418 2312 14-15 0.8786158438414446 2312 16-17 0.8908146491491138 2312 18-19 0.8608027564314682 2312 20-21 0.8546100519079971 2312 22-23 0.930351556898934 2312 24-25 0.9105783507326208 2312 26-27 0.979643667654635 2312 28-29 0.9661232771161536 2312 30-31 0.9519053132667779 2312 32-33 0.829015064102002 2312 34-35 0.9471241481583874 2312 36-37 0.999807670057173 2312 38-39 0.7735320718077432 2312 40-41 0.9331931647591318 2312 42-43 0.9893724365987779 2312 44-45 0.7769835482191709 2312 46-47 0.7937204387105083 2312 48-49 0.946134854835762 2312 50-51 0.8417319326888801 2312 52-53 0.9060747663239326 2312 54-55 0.8519674960127119 2312 56-57 0.8798413175362398 2312 58-59 1.013871369123656 2312 60-61 1.0333404226981955 2312 62-63 1.0330680597459931 2312 64-65 1.0220474954879464 2312 66-67 1.0298976710630114 2312 68-69 1.0793997684879741 2312 70-71 1.0119795286188378 2312 72-73 1.0278318063378293 2312 74-75 1.0500845227262872 2312 76-77 1.0248648466433963 2312 78-79 0.9736744026714277 2312 80-81 0.955379265626263 2312 82-83 0.9673503372947252 2312 84-85 1.054158014567097 2312 86-87 1.0912646249705915 2312 88-89 1.0668166526653557 2312 90-91 1.1031000610074564 2312 92-93 1.1341993660827079 2312 94-95 1.1466764533586797 2312 96-97 0.8234367062138332 2312 98-99 0.6131856061948469 2312 100 0.8782420991504232 2313 1 0.042487471876384575 2313 2 0.23249799856417752 2313 3 0.2868985234671939 2313 4 0.1833083323621807 2313 5 0.316014338391696 2313 6 0.2796066299409574 2313 7 0.2517926852105177 2313 8 0.3833446967007461 2313 9 0.5768901282792243 2313 10-11 0.35134147868566856 2313 12-13 0.4160109036765647 2313 14-15 0.46263462497170593 2313 16-17 0.7252538295162694 2313 18-19 0.7040304480250725 2313 20-21 0.6715653327761189 2313 22-23 0.6350907814731883 2313 24-25 0.5017453809819088 2313 26-27 0.7295740064926477 2313 28-29 0.5324094664860652 2313 30-31 0.7792791054969115 2313 32-33 0.8719772671415811 2313 34-35 0.9379199109232417 2313 36-37 0.9877968313378744 2313 38-39 0.9398644386846868 2313 40-41 0.9700909523490537 2313 42-43 1.0174377682197928 2313 44-45 0.9051861716328418 2313 46-47 0.904075552676062 2313 48-49 0.9751804985562842 2313 50-51 0.9455540239295317 2313 52-53 0.9951550225414536 2313 54-55 0.96693366511996 2313 56-57 0.7249757955535969 2313 58-59 0.7880539586539683 2313 60-61 0.7009398629821035 2313 62-63 0.9542939251185416 2313 64-65 1.0692418122521516 2313 66-67 0.9911294307154392 2313 68-69 1.0901439027393778 2313 70-71 1.0293680778651577 2313 72-73 1.0895410332689153 2313 74-75 1.1897939730978067 2313 76-77 1.075009569142182 2313 78-79 0.9663325425276454 2313 80-81 0.9159080323793773 2313 82-83 0.9892489070354671 2313 84-85 0.9772031458175725 2313 86-87 0.9737096650739971 2313 88-89 0.9964804414979689 2313 90-91 0.8267186902769303 2313 92-93 1.0721602256375 2313 94-95 1.2162278628043026 2313 96-97 1.2593459912569216 2313 98-99 1.2516110666198728 2313 100 1.3985916850422804 2314 1 -0.32260784324519776 2314 2 0.020125411796634296 2314 3 0.2690855075826164 2314 4 0.2012570752170859 2314 5 0.32560136990498023 2314 6 0.3876951785006071 2314 7 0.40603741557109885 2314 8 0.41518583381772345 2314 9 0.4213783460431699 2314 10-11 0.5337198260472462 2314 12-13 0.4550815053550963 2314 14-15 0.7426469825926176 2314 16-17 0.7688666708292047 2314 18-19 0.812322865851165 2314 20-21 0.8426341635554095 2314 22-23 0.8940598420016883 2314 24-25 0.8360535947975336 2314 26-27 0.7116315863506273 2314 28-29 0.7492301087495861 2314 30-31 0.8045056146623679 2314 32-33 0.832800245121355 2314 34-35 0.8967392499704019 2314 36-37 0.8814941658602038 2314 38-39 0.6490793250310034 2314 40-41 0.5818060534247707 2314 42-43 0.744015129939676 2314 44-45 0.8296561317763604 2314 46-47 0.8256203047569102 2314 48-49 0.8017373002169137 2314 50-51 0.7883009278912994 2314 52-53 0.83401639458814 2314 54-55 0.7267520782449353 2314 56-57 0.6795668855650163 2314 58-59 0.8299606703081324 2314 60-61 0.900150261371472 2314 62-63 0.8418166583292859 2314 64-65 0.8140939187813174 2314 66-67 0.5717194126781706 2314 68-69 0.5466348825305261 2314 70-71 0.7264884958657305 2314 72-73 0.8463914138683464 2314 74-75 0.9168169376766713 2314 76-77 0.7376461673967327 2314 78-79 0.6847472144593425 2314 80-81 0.7325146242797373 2314 82-83 0.7411843676963699 2314 84-85 0.789391577951168 2314 86-87 0.8413970406489923 2314 88-89 0.8070542928980338 2314 90-91 0.9110976276384513 2314 92-93 0.8305648828596688 2314 94-95 0.8327677019663611 2314 96-97 0.8442550516685081 2314 98-99 0.9273608724640212 2314 100 0.9815212686381258 2315 1 0.3884782157519098 2315 2 0.4303657260252862 2315 3 0.43642292813184724 2315 4 0.3035197244647847 2315 5 0.3730979701977901 2315 6 0.4306017222748153 2315 7 0.3800870043345057 2315 8 0.31014261357945827 2315 9 0.5369204852746989 2315 10-11 0.5480967659947282 2315 12-13 0.40631584277608823 2315 14-15 0.6209513533140552 2315 16-17 0.762908238286748 2315 18-19 0.7586116008128911 2315 20-21 0.4442142717851709 2315 22-23 0.8390948762346895 2315 24-25 0.8511761111194645 2315 26-27 0.8205463374965092 2315 28-29 0.7974302559107258 2315 30-31 0.8296226469394483 2315 32-33 0.7843315889749789 2315 34-35 0.8649224375447631 2315 36-37 0.8196038529892107 2315 38-39 0.8671247126243316 2315 40-41 0.9206078385881966 2315 42-43 0.9762887116664132 2315 44-45 1.0147908625618385 2315 46-47 0.9561353776452961 2315 48-49 0.8411164413496124 2315 50-51 0.9256578719963287 2315 52-53 0.9530658920991684 2315 54-55 0.8351859249507427 2315 56-57 0.7925756141880314 2315 58-59 0.8088622722100993 2315 60-61 0.8895233130777385 2315 62-63 0.9735174556079116 2315 64-65 1.048335284415323 2315 66-67 1.062372080786183 2315 68-69 1.0417420684543401 2315 70-71 0.9521547294174937 2315 72-73 1.0106867563919053 2315 74-75 0.4370208132761064 2315 76-77 0.45599303056313545 2315 78-79 0.7538542276713542 2315 80-81 0.8614629117843506 2315 82-83 0.7299933239606631 2315 84-85 0.7649282375891779 2315 86-87 0.8154106626247 2315 88-89 0.799829624667165 2315 90-91 0.8377961703158512 2315 92-93 0.941771524073598 2315 94-95 0.9533795379773977 2315 96-97 0.9981147731840814 2315 98-99 1.0170407546994973 2315 100 1.0062828618065716 2316 1 0.26593679482678567 2316 2 0.028358490164343664 2316 3 0.19395567016234594 2316 4 0.006962871308410001 2316 5 0.24453198161669576 2316 6 0.3632078133014929 2316 7 0.41975658237122815 2316 8 0.4450418821755946 2316 9 0.6072614221293406 2316 10-11 0.6098328722407871 2316 12-13 0.40033922523051757 2316 14-15 0.4657639306639112 2316 16-17 0.7582987020156011 2316 18-19 0.7391375879463951 2316 20-21 0.7052995996826255 2316 22-23 0.7560666114598646 2316 24-25 0.46665554511010043 2316 26-27 0.7384032813188028 2316 28-29 0.8496445881965045 2316 30-31 0.5345838401087732 2316 32-33 0.64703032438468 2316 34-35 0.8354298349120697 2316 36-37 0.8649947165609007 2316 38-39 0.7684075059650368 2316 40-41 0.7721932349376885 2316 42-43 0.7583246693642067 2316 44-45 0.6056100339672668 2316 46-47 0.7487976285930813 2316 48-49 0.8711000084296501 2316 50-51 0.8683309905128098 2316 52-53 0.8065517919828906 2316 54-55 0.7044663527113002 2316 56-57 0.8403410964883022 2316 58-59 0.7084880760559926 2316 60-61 0.8446861830726604 2316 62-63 0.8652646799672183 2316 64-65 0.8929579364180498 2316 66-67 0.803157139657678 2316 68-69 0.8458451660944206 2316 70-71 0.9170027814266319 2316 72-73 0.895738652357096 2316 74-75 0.891413888288973 2316 76-77 0.7452550437436258 2316 78-79 0.5066736737714663 2316 80-81 0.49146945608563186 2316 82-83 0.4078439183496485 2316 84-85 0.5641334643985694 2316 86-87 0.6346034787025445 2316 88-89 0.6128454274667341 2316 90-91 0.464814682897309 2316 92-93 0.3799614025743949 2316 94-95 0.4172356517224145 2316 96-97 0.6340347713882473 2316 98-99 0.7536218518075977 2316 100 0.3670205631507102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 8.0 12 37.0 13 185.0 14 731.0 15 2304.0 16 5473.0 17 11665.0 18 20080.0 19 29883.0 20 44372.0 21 64239.0 22 77352.0 23 76457.0 24 73048.0 25 76205.0 26 88763.0 27 108801.0 28 135282.0 29 166515.0 30 205769.0 31 257549.0 32 327533.0 33 437550.0 34 641992.0 35 890365.0 36 1393712.0 37 1970982.0 38 1503525.0 39 262338.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26545997404404 22.06136745718451 13.825729771662004 22.84744279710944 2 15.144544527599333 20.20379740943405 41.59657458417829 23.055083478788326 3 18.627631418639556 26.95136887360411 29.184863333541085 25.236136374215253 4 12.123546551775648 16.778741027897297 39.922298617057436 31.175413803269613 5 12.65835453405394 38.884174695665926 34.65642435087363 13.801046419406513 6 29.376382677130035 38.67101602178269 17.88310321070182 14.069498090385455 7 26.988213012864453 32.348259249249324 23.088866412868466 17.574661325017757 8 26.5131160827222 35.49390024471603 19.54447406453006 18.448509608031706 9 26.457198436343067 15.21145674813146 21.970358328842497 36.360986486682975 10-11 26.014399012160005 26.50847679599382 26.746166777794155 20.730957414052014 12-13 25.838248016507205 24.088392391430283 27.860054828659315 22.2133047634032 14-15 23.209778016762506 25.305657414279803 25.711322216373457 25.773242352584237 16-17 21.390468624201645 27.95065970005883 27.13203124661004 23.52684042912949 18-19 20.80236720748838 27.81299178054834 29.837948465258673 21.54669254670461 20-21 22.49592473298668 26.94733859578643 29.1661423542457 21.39059431698119 22-23 22.770362863392823 26.712310626274906 28.85623291042343 21.661093599908845 24-25 22.075377642210633 26.61324352360571 29.86847610035475 21.442902733828905 26-27 22.01043044256517 26.980483465991455 28.793154088019833 22.215932003423543 28-29 21.176823686641992 27.50888285140263 29.311223149985448 22.003070311969932 30-31 22.2779060327526 26.949021343098018 29.234443844965302 21.53862877918408 32-33 22.61551345635749 27.243542071678302 28.30314353374718 21.837800938217033 34-35 22.468839687148904 27.09434887961311 29.24884148638768 21.18796994685031 36-37 22.068804571375743 26.685062053426854 28.95887841880095 22.287254956396453 38-39 22.278651077918152 26.806387549563134 29.527102365671837 21.38785900684688 40-41 21.88627204705897 25.874905833817596 30.09875220510037 22.140069914023066 42-43 21.94499874925692 26.14553442456887 29.232810143850386 22.676656682323827 44-45 21.958875882475404 27.15281279049282 29.048729470995493 21.839581856036283 46-47 22.04490319374901 26.84464613642412 28.364118251329334 22.74633241849753 48-49 22.926885924942773 26.797257147932246 28.320726978988368 21.95512994813661 50-51 22.46378947502495 26.57877697272059 28.228848951073935 22.728584601180522 52-53 21.750091667050846 27.277493662208297 27.966994451283234 23.005420219457626 54-55 21.83265407682122 27.221839456504615 27.846011520167902 23.099494946506262 56-57 22.900071167743256 26.621503410546023 27.45214125644625 23.02628416526447 58-59 21.75590579226616 27.196564505192082 28.315532698044 22.73199700449776 60-61 22.021417183677542 27.9042673755384 27.499683002688702 22.574632438095357 62-63 22.458680039043717 26.991614707653667 27.169983697597278 23.379721555705345 64-65 21.502972163895866 27.238215745591248 27.54427128431033 23.714540806202557 66-67 22.043064137786025 26.830250102016922 27.59174714260993 23.534938617587127 68-69 21.626357573145373 26.88440839661514 27.81987730897105 23.669356721268436 70-71 21.602654576817017 26.42713583450358 28.051870538354905 23.918339050324498 72-73 22.18721395181119 26.63091583454527 28.133588492921962 23.048281720721576 74-75 22.394972163816192 27.017262949354148 27.588970288096142 22.998794598733515 76-77 22.024823241143114 26.81921769056439 27.330086130836168 23.825872937456328 78-79 21.852299085573513 26.844556514848406 27.47509907531584 23.82804532426224 80-81 21.398666925664866 27.322444927239033 27.982383163268555 23.296504983827546 82-83 22.494063281551423 26.172201703433995 29.00795861896967 22.32577639604491 84-85 22.663290769263817 26.707483474165812 27.598451787154733 23.03077396941564 86-87 21.152123057679397 27.49721135139972 28.056982199279147 23.29368339164174 88-89 22.118257705171665 26.260829483160585 27.9655653549472 23.655347456720555 90-91 21.94198632660165 26.19009093009486 28.156221242896596 23.711701500406893 92-93 22.25790348526527 26.518858519037792 27.84094627882179 23.38229171687515 94-95 20.988816027630705 26.49219341381092 28.510689669184615 24.008300889373757 96-97 21.34498842393721 26.445105883653735 28.07584213229256 24.134063560116488 98-99 20.66732947869753 26.948077513497807 28.008546701672188 24.37604630613247 100 21.36895895650778 26.359168550564448 27.2334955447114 25.038376948216374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1914.0 1 1310.0 2 1007.5 3 1299.5 4 1016.5 5 867.5 6 1353.0 7 2658.0 8 4082.5 9 4612.0 10 4103.0 11 3545.5 12 3874.0 13 4780.5 14 6008.5 15 7475.5 16 9036.0 17 10530.0 18 11790.0 19 12893.5 20 14049.0 21 15133.5 22 16316.0 23 17923.0 24 19885.0 25 22128.0 26 26022.5 27 30935.5 28 38379.5 29 54887.5 30 78696.0 31 101606.0 32 135103.5 33 171421.0 34 197867.0 35 227730.5 36 255005.0 37 295700.0 38 311953.0 39 290160.0 40 265417.5 41 239477.5 42 231730.5 43 242627.0 44 264865.0 45 315591.5 46 362101.0 47 389455.5 48 413603.5 49 410609.5 50 390909.5 51 394264.0 52 391522.5 53 364251.5 54 341213.5 55 294060.0 56 251464.5 57 204752.0 58 156964.0 59 148660.0 60 122153.0 61 79893.0 62 57012.0 63 47872.0 64 33946.0 65 14904.0 66 7502.0 67 4898.0 68 3661.5 69 2831.5 70 2219.5 71 1761.0 72 1463.5 73 1420.0 74 1165.5 75 859.0 76 578.0 77 344.0 78 206.0 79 134.5 80 89.5 81 57.0 82 47.5 83 32.5 84 17.0 85 9.0 86 5.0 87 2.5 88 2.5 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04353788173271978 2 0.0 3 0.0 4 0.03243645447237058 5 0.0 6 0.0 7 7.551224633943115E-4 8 0.0 9 0.010887288054312015 10-11 4.620898656592056E-4 12-13 1.1270484528273307E-5 14-15 1.5215154113168964E-4 16-17 2.9866783999924265E-4 18-19 0.0 20-21 1.690572679240996E-5 22-23 0.0 24-25 5.52253741885392E-4 26-27 5.747947109419387E-4 28-29 2.1413920603719283E-4 30-31 0.0024344246581070343 32-33 0.015006650149395907 34-35 0.026091171682952705 36-37 0.031055820117657097 38-39 0.022270477427868054 40-41 0.0013637286279210702 42-43 8.45286339620498E-5 44-45 0.01077458320902928 46-47 0.01758195586410636 48-49 0.003262805270935122 50-51 0.010915464265632698 52-53 0.010729501270916188 54-55 0.010149071317710113 56-57 3.7756123169715576E-4 58-59 0.00862192066412908 60-61 0.004711062532818242 62-63 0.0012904704784872936 64-65 0.03494977252217552 66-67 0.06008858826248914 68-69 0.059226396196076225 70-71 0.05159064292817107 72-73 0.04547640507158279 74-75 0.03462856371311974 76-77 0.03086985712294059 78-79 0.014939027242226267 80-81 9.523559426390944E-4 82-83 0.03781247559235694 84-85 0.05153429050552969 86-87 0.057327319553062174 88-89 0.06919513976133396 90-91 0.048147509904783566 92-93 0.03905786413273114 94-95 0.01300050390336326 96-97 0.0037136246520660544 98-99 0.021892916196170897 100 0.03183911879237209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8872733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.43218552870813 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22793794189037 54.21740948296417 2 13.570597128376304 18.573312408488025 3 3.986626642147158 8.184407220273151 4 1.4638584474855034 4.0070013146437855 5 0.6683046495857687 2.2866773885075804 6 0.34323766837066955 1.4093102281429712 7 0.19655384946467783 0.9415426645064022 8 0.12333651323568633 0.6752149724966763 9 0.073735171216913 0.4541273025036166 >10 0.3059577210628399 3.6030678798270612 >50 0.021964125365866043 1.0464344831328516 >100 0.015913596992532402 2.1523495158181296 >500 0.0013383406825437263 0.6299663807120501 >1k 5.558552057706402E-4 0.6962151730622571 >5k 2.0587229329403613E-5 0.09050318119075385 >10k+ 6.176168798821083E-5 1.0324604037304477 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30912 0.3483932177379845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25749 0.2902037061185094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16624 0.18736053479801545 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1270484528273307E-5 0.0 3 0.0 0.0 0.0 1.1270484528273307E-5 0.0 4 0.0 0.0 0.0 1.1270484528273307E-5 0.0 5 0.0 0.0 0.0 1.1270484528273307E-5 0.0 6 0.0 0.0 0.0 1.1270484528273307E-5 1.1270484528273307E-5 7 0.0 0.0 0.0 1.1270484528273307E-5 1.1270484528273307E-5 8 0.0 0.0 0.0 1.1270484528273307E-5 1.1270484528273307E-5 9 0.0 0.0 0.0 2.2540969056546613E-5 2.2540969056546613E-5 10-11 0.0 0.0 0.0 5.071718037722987E-5 2.2540969056546613E-5 12-13 0.0 0.0 0.0 8.45286339620498E-5 2.2540969056546613E-5 14-15 0.0 0.0 0.0 1.0143436075445976E-4 4.508193811309323E-5 16-17 0.0 0.0 0.0 1.1270484528273306E-4 6.762290716963983E-5 18-19 0.0 0.0 0.0 1.6342202565996296E-4 6.762290716963983E-5 20-21 0.0 0.0 0.0 2.423154173578761E-4 6.762290716963983E-5 22-23 0.0 0.0 0.0 6.198766490550318E-4 7.325814943377649E-5 24-25 0.0 0.0 0.0 0.0013580933856569334 7.889339169791315E-5 26-27 0.0 0.0 0.0 0.002406248446786351 7.889339169791315E-5 28-29 0.0 0.0 0.0 0.005978992042248989 7.889339169791315E-5 30-31 0.0 0.0 0.0 0.019441585811271454 7.889339169791315E-5 32-33 1.1270484528273307E-5 0.0 0.0 0.05047486495987201 7.889339169791315E-5 34-35 1.1270484528273307E-5 0.0 0.0 0.0950270903001364 9.016387622618645E-5 36-37 1.1270484528273307E-5 1.1270484528273307E-5 0.0 0.1686120837852328 9.016387622618645E-5 38-39 1.1270484528273307E-5 1.1270484528273307E-5 0.0 0.2765213379011856 9.016387622618645E-5 40-41 1.1270484528273307E-5 1.1270484528273307E-5 0.0 0.38990241225561506 1.070696030185964E-4 42-43 1.1270484528273307E-5 1.1270484528273307E-5 0.0 0.49998686988552454 1.1270484528273306E-4 44-45 2.2540969056546613E-5 1.1270484528273307E-5 0.0 0.6278899635546342 1.1270484528273306E-4 46-47 2.2540969056546613E-5 1.1270484528273307E-5 0.0 0.7794328985217971 1.1270484528273306E-4 48-49 2.2540969056546613E-5 1.1270484528273307E-5 0.0 0.9488001047704242 1.1270484528273306E-4 50-51 3.381145358481992E-5 1.1270484528273307E-5 0.0 1.1258143347714848 1.1270484528273306E-4 52-53 3.381145358481992E-5 1.1270484528273307E-5 0.0 1.29287672693408 1.1834008754686972E-4 54-55 3.381145358481992E-5 1.1270484528273307E-5 0.0 1.4725507912838132 1.2397532981100638E-4 56-57 3.381145358481992E-5 1.1270484528273307E-5 0.0 1.6790204325995157 1.2397532981100638E-4 58-59 3.381145358481992E-5 1.1270484528273307E-5 0.0 1.920017203267584 1.29610572075143E-4 60-61 3.381145358481992E-5 1.1270484528273307E-5 0.0 2.166412536024695 1.3524581433927967E-4 62-63 3.381145358481992E-5 1.1270484528273307E-5 0.0 2.415394444981045 1.3524581433927967E-4 64-65 3.381145358481992E-5 1.1270484528273307E-5 0.0 2.6665121107555025 1.3524581433927967E-4 66-67 3.381145358481992E-5 1.1270484528273307E-5 0.0 2.9451635702325314 1.3524581433927967E-4 68-69 3.381145358481992E-5 1.1270484528273307E-5 0.0 3.2494553820113827 1.3524581433927967E-4 70-71 3.381145358481992E-5 1.1270484528273307E-5 0.0 3.579241029793188 1.3524581433927967E-4 72-73 3.381145358481992E-5 1.1270484528273307E-5 0.0 3.9131911216081896 1.4651629886755298E-4 74-75 3.381145358481992E-5 1.1270484528273307E-5 0.0 4.227964483998335 1.4651629886755298E-4 76-77 3.381145358481992E-5 1.1270484528273307E-5 0.0 4.582922758974039 1.4651629886755298E-4 78-79 3.381145358481992E-5 1.1270484528273307E-5 0.0 4.963279070834206 1.4651629886755298E-4 80-81 3.381145358481992E-5 1.1270484528273307E-5 0.0 5.372966818679205 1.4651629886755298E-4 82-83 3.381145358481992E-5 1.1270484528273307E-5 0.0 5.78828980878834 1.4651629886755298E-4 84-85 3.381145358481992E-5 1.1270484528273307E-5 0.0 6.180108203413763 1.4651629886755298E-4 86-87 3.381145358481992E-5 1.1270484528273307E-5 0.0 6.592624842875358 1.4651629886755298E-4 88 3.381145358481992E-5 1.1270484528273307E-5 0.0 6.926095939097908 1.4651629886755298E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19635 0.0 43.55325 1 GGGCACC 8790 0.0 38.722076 94 GTATCAA 33475 0.0 37.652603 1 GTACTAG 9500 0.0 35.136097 1 TAGGCAT 11595 0.0 31.852272 5 CAACGCA 38120 0.0 31.703463 5 ATCAACG 38730 0.0 31.091223 3 TACCTGG 4150 0.0 30.236404 2 CTGTGCG 12375 0.0 30.186176 9 TATCAAC 42795 0.0 29.398308 2 TAGTACT 13025 0.0 28.832027 4 ACCTGGG 4225 0.0 28.70114 3 ACTGTGC 13415 0.0 28.616568 8 CTAGGCA 13395 0.0 28.386507 4 AACGCAG 44105 0.0 28.285599 6 CTAACGC 4475 0.0 27.727901 3 TATTCTC 4805 0.0 27.674623 5 GTGGTAT 6525 0.0 27.52337 1 GCATCGT 11645 0.0 27.236446 8 TACTGTG 15235 0.0 27.048662 7 >>END_MODULE