FastQCFastQC Report
Mon 6 Jun 2016
ERR1211203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280025
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269640.32565119066669485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208580.2519074519702537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134550.16249950936138477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142400.045.2722131
GTATCAA249800.040.3504331
GTACTAG65000.035.3286931
CAACGCA286600.033.4255945
ATCAACG292550.032.9223183
GCATCGT66300.032.0442288
TATCAAC316600.031.6733262
AACGCAG323700.030.3061586
TACCTGG56900.030.0203322
GTGGTAT46800.029.963431
TCAACGC333250.029.1694974
TAGGCAT83800.028.4951445
GTACATG345200.028.3948521
TACATGG352900.027.4019372
ACGCAGA349200.027.2990477
CGCAGAG356250.026.7834248
CTAGGCA90250.026.5626564
CTGTGCG94000.026.5516579
TAGTACT95550.026.4175244
ACATGGG352300.026.3647023
Produced by FastQC (version 0.11.5)