##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211203_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8280025 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41803557356409 31.0 31.0 34.0 28.0 34.0 2 31.434552794224654 31.0 31.0 34.0 28.0 34.0 3 31.497539802114122 33.0 31.0 34.0 28.0 34.0 4 35.09349850996826 37.0 35.0 37.0 32.0 37.0 5 35.148494479183334 37.0 35.0 37.0 32.0 37.0 6 35.20991760290579 37.0 35.0 37.0 32.0 37.0 7 35.123142623361645 37.0 35.0 37.0 32.0 37.0 8 35.12830770438495 37.0 35.0 37.0 32.0 37.0 9 36.68781966238025 39.0 37.0 39.0 32.0 39.0 10-11 36.63774149232641 39.0 37.0 39.0 32.0 39.0 12-13 36.52270886863265 39.0 37.0 39.0 32.0 39.0 14-15 37.71097116252668 40.0 37.0 41.0 32.0 41.0 16-17 37.688104746060546 40.0 37.0 41.0 32.0 41.0 18-19 37.6941167448166 40.0 37.0 41.0 32.0 41.0 20-21 37.65780302595705 40.0 37.0 41.0 32.0 41.0 22-23 37.548976724104094 40.0 37.0 41.0 32.0 41.0 24-25 37.44900130132457 40.0 37.0 41.0 31.0 41.0 26-27 37.295390050150814 40.0 36.5 41.0 31.0 41.0 28-29 37.176501278679716 40.0 36.0 41.0 31.0 41.0 30-31 37.02307227333251 39.5 36.0 41.0 30.0 41.0 32-33 36.83862844617015 39.0 35.5 41.0 30.0 41.0 34-35 36.69520357728389 39.0 35.0 41.0 30.0 41.0 36-37 36.58916645783074 39.0 35.0 41.0 30.0 41.0 38-39 36.44338610088738 39.0 35.0 41.0 29.5 41.0 40-41 36.268563198782616 39.0 35.0 40.0 29.0 41.0 42-43 36.05317568485602 39.0 35.0 40.0 28.0 41.0 44-45 35.825519427779504 38.0 35.0 40.0 27.0 41.0 46-47 35.58191726474256 38.0 34.0 40.0 26.5 41.0 48-49 35.36687147683733 38.0 34.0 40.0 26.0 41.0 50-51 34.667657645961214 37.5 33.5 39.5 25.0 40.5 52-53 34.76246080174879 37.5 33.5 39.5 25.5 40.5 54-55 35.232731120980915 38.0 34.0 40.0 26.0 41.0 56-57 35.17260914067289 38.0 34.0 40.0 26.0 41.0 58-59 34.93984994006661 37.0 34.0 40.0 26.0 41.0 60-61 34.70526260488344 37.0 33.5 40.0 26.0 41.0 62-63 34.39252236557257 36.0 33.0 40.0 26.0 41.0 64-65 34.13006989713195 36.0 33.0 39.0 25.0 41.0 66-67 33.951767899251514 35.5 33.0 39.0 25.0 41.0 68-69 33.655439325364355 35.0 33.0 39.0 25.0 41.0 70-71 33.36693403703491 35.0 32.5 38.0 24.5 40.0 72-73 33.04547975398625 35.0 32.0 37.0 24.0 40.0 74-75 32.72907932041268 35.0 32.0 37.0 23.5 39.0 76-77 32.42037644813874 35.0 32.0 36.0 23.0 39.0 78-79 32.10662123604699 35.0 31.0 36.0 23.0 39.0 80-81 31.83486481019079 35.0 31.0 35.5 22.0 37.5 82-83 31.56425493884378 35.0 31.0 35.0 20.5 37.0 84-85 31.337421928073887 34.0 31.0 35.0 20.0 37.0 86-87 31.128763922814244 34.0 31.0 35.0 20.0 36.0 88-89 30.93060576507921 34.0 30.0 35.0 20.0 36.0 90-91 30.717882131998394 34.0 30.0 35.0 19.0 36.0 92-93 30.527431318142156 34.0 30.0 35.0 18.5 35.0 94-95 30.36326502637371 34.0 29.5 35.0 18.0 35.0 96-97 30.18491320980311 34.0 29.0 35.0 18.0 35.0 98-99 29.95567531498999 34.0 29.0 35.0 17.0 35.0 100 28.806973529669296 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4980134976552719 1101 2 -0.45278283636299577 1101 3 -0.4643166865518005 1101 4 -0.4527995046968414 1101 5 -0.3400026813605095 1101 6 -0.29927610141260175 1101 7 -0.3509223205772827 1101 8 -0.3175408761159275 1101 9 -0.482247743485793 1101 10-11 -0.4494861755392492 1101 12-13 -0.4652042396970302 1101 14-15 -0.6790412455866317 1101 16-17 -0.7465327304636631 1101 18-19 -0.6578789043440807 1101 20-21 -0.7341545156948044 1101 22-23 -0.6848049958365223 1101 24-25 -0.8234762617688034 1101 26-27 -0.8223204019305257 1101 28-29 -0.7782253675244775 1101 30-31 -0.922019161854081 1101 32-33 -0.923906362013831 1101 34-35 -0.8569676749907202 1101 36-37 -0.8997162709427329 1101 38-39 -0.8411133718795796 1101 40-41 -0.8744627513085774 1101 42-43 -1.0388240641701358 1101 44-45 -0.9870315457618162 1101 46-47 -1.1366803444177336 1101 48-49 -1.208476710748343 1101 50-51 -1.2595576980735714 1101 52-53 -1.057251484425315 1101 54-55 -0.9778673744754585 1101 56-57 -0.8267173291300693 1101 58-59 -0.9061893619027828 1101 60-61 -0.8480985863626103 1101 62-63 -0.9562572819995125 1101 64-65 -0.9159586709362557 1101 66-67 -0.9298313854424478 1101 68-69 -0.8579058457394027 1101 70-71 -0.976655269792289 1101 72-73 -0.930909332611705 1101 74-75 -0.9734949152413996 1101 76-77 -0.9957542243535862 1101 78-79 -1.0055021752574227 1101 80-81 -1.091927770257744 1101 82-83 -1.011091962583908 1101 84-85 -1.1131877668836196 1101 86-87 -1.199014156036803 1101 88-89 -1.211315087636951 1101 90-91 -1.33491251032099 1101 92-93 -1.3880731921701752 1101 94-95 -1.5149758606708836 1101 96-97 -1.4220895227289923 1101 98-99 -1.5634979432920595 1101 100 -1.5814441881105594 1102 1 -0.47915196576249386 1102 2 -0.4303578653093041 1102 3 -0.4655672366186039 1102 4 -0.2625821560577464 1102 5 -0.29772802077057037 1102 6 -0.40206609323592346 1102 7 -0.3669454332374258 1102 8 -0.40685938620129747 1102 9 -0.48907362326557546 1102 10-11 -0.5798610507235651 1102 12-13 -0.5791061606122909 1102 14-15 -0.6731705318636685 1102 16-17 -0.7099756343990222 1102 18-19 -0.6924824496478053 1102 20-21 -0.8134508541752297 1102 22-23 -0.7881822445270004 1102 24-25 -0.7645326185867205 1102 26-27 -0.8842606732465796 1102 28-29 -0.950469000785894 1102 30-31 -0.9631965043850812 1102 32-33 -0.9490286685497296 1102 34-35 -0.9732241550964815 1102 36-37 -0.9972073369634984 1102 38-39 -1.0701412976345992 1102 40-41 -1.1018770373612128 1102 42-43 -1.0716067289462998 1102 44-45 -1.258323018976938 1102 46-47 -1.17265080469177 1102 48-49 -1.20922049546963 1102 50-51 -1.194159827322757 1102 52-53 -1.0920116486743936 1102 54-55 -0.8563360497754786 1102 56-57 -0.8172561552755013 1102 58-59 -0.9745138778812645 1102 60-61 -0.894120061036844 1102 62-63 -0.9849930119375401 1102 64-65 -1.0065973413488152 1102 66-67 -0.9632919180147681 1102 68-69 -1.0247583026271698 1102 70-71 -1.0171558624649393 1102 72-73 -1.0827594120184187 1102 74-75 -1.1045219704955187 1102 76-77 -1.2196163831196358 1102 78-79 -1.1094290932619018 1102 80-81 -1.2111422640977416 1102 82-83 -1.1975176140062622 1102 84-85 -1.3339979223299743 1102 86-87 -1.2810394552176625 1102 88-89 -1.3048328784991092 1102 90-91 -1.4503827157880202 1102 92-93 -1.350682528557332 1102 94-95 -1.4664194744553392 1102 96-97 -1.464769464755225 1102 98-99 -1.6012602922400276 1102 100 -1.7261979710050532 1103 1 -0.5024826649546839 1103 2 -0.3897528707509679 1103 3 -0.5400274862587686 1103 4 -0.40595796974385934 1103 5 -0.356521181782405 1103 6 -0.3937215412949371 1103 7 -0.35275602292770003 1103 8 -0.3803964888799385 1103 9 -0.5708465768029001 1103 10-11 -0.5179867477258497 1103 12-13 -0.6053655357026102 1103 14-15 -0.7165642461856123 1103 16-17 -0.7523512996347819 1103 18-19 -0.670248329657646 1103 20-21 -0.6919255411938039 1103 22-23 -0.7173753969983636 1103 24-25 -0.7950328347676461 1103 26-27 -0.7798616927961959 1103 28-29 -0.7421249181747172 1103 30-31 -0.7440830046202365 1103 32-33 -0.825984610890707 1103 34-35 -0.9126807574325184 1103 36-37 -0.8613884852506146 1103 38-39 -0.8462749576104684 1103 40-41 -0.8966380589976879 1103 42-43 -0.9642067478814482 1103 44-45 -1.0159323880692597 1103 46-47 -0.9680956266510918 1103 48-49 -1.0508039914667862 1103 50-51 -0.9959303806677227 1103 52-53 -0.9278325264378182 1103 54-55 -0.8171954138233275 1103 56-57 -0.8353565620109293 1103 58-59 -0.744009669091767 1103 60-61 -0.7898969305365711 1103 62-63 -0.7363620957645836 1103 64-65 -0.726749844784635 1103 66-67 -0.8039015133273679 1103 68-69 -0.7729862148711533 1103 70-71 -0.7501437762140881 1103 72-73 -0.8901117263605869 1103 74-75 -0.8691226765030713 1103 76-77 -0.9446615424808229 1103 78-79 -0.9109839366737269 1103 80-81 -0.9185912736027753 1103 82-83 -0.8572783843695149 1103 84-85 -0.9901918769752278 1103 86-87 -1.0765930410569382 1103 88-89 -1.0470562776080783 1103 90-91 -1.147138417240516 1103 92-93 -1.1602446674698008 1103 94-95 -1.2525697729995038 1103 96-97 -1.2048701792983003 1103 98-99 -1.2871876376377038 1103 100 -1.318249388003487 1104 1 -0.4989937725444449 1104 2 -0.45081358745427735 1104 3 -0.5504303985727752 1104 4 -0.32759397519499345 1104 5 -0.40055985396915617 1104 6 -0.36685512841152956 1104 7 -0.4366786823600606 1104 8 -0.3760283917764511 1104 9 -0.505578811141774 1104 10-11 -0.5253149140867208 1104 12-13 -0.5248149887440192 1104 14-15 -0.7244773221182328 1104 16-17 -0.7179632639763156 1104 18-19 -0.7463802683509897 1104 20-21 -0.7511637914771399 1104 22-23 -0.7539050992390699 1104 24-25 -0.744608356557869 1104 26-27 -0.805982607002818 1104 28-29 -0.7427204607967184 1104 30-31 -0.7933504750931988 1104 32-33 -0.9831916297521559 1104 34-35 -0.9830798128036875 1104 36-37 -1.013679529496443 1104 38-39 -1.0072193684410848 1104 40-41 -0.9828095334417029 1104 42-43 -0.9724493899483875 1104 44-45 -1.000633940966992 1104 46-47 -1.0923966600884256 1104 48-49 -1.0220908497795165 1104 50-51 -1.1398646903843783 1104 52-53 -0.943108689204017 1104 54-55 -0.8571496175930662 1104 56-57 -0.7908038978567973 1104 58-59 -0.8222426534446043 1104 60-61 -0.8197543269772893 1104 62-63 -0.8510010917736182 1104 64-65 -0.7802918014571745 1104 66-67 -0.7878178897454191 1104 68-69 -0.9429491883200285 1104 70-71 -0.8885161743780543 1104 72-73 -0.7761282403723229 1104 74-75 -0.9014681148936319 1104 76-77 -0.8727752038120968 1104 78-79 -0.8995985355245466 1104 80-81 -0.9101703401548384 1104 82-83 -0.9775209310895434 1104 84-85 -0.9647175173239724 1104 86-87 -1.0460966433657646 1104 88-89 -1.1252360299401332 1104 90-91 -1.139238069252663 1104 92-93 -1.2310953603983101 1104 94-95 -1.1416059647810712 1104 96-97 -1.2614114907600253 1104 98-99 -1.2592652977643901 1104 100 -1.2684166715901597 1105 1 -0.5895206435174245 1105 2 -0.5505504613530938 1105 3 -0.5719523594424025 1105 4 -0.36551199587547956 1105 5 -0.35579539463894605 1105 6 -0.3331584282486233 1105 7 -0.30962830740947567 1105 8 -0.4330778965964299 1105 9 -0.5528913701746418 1105 10-11 -0.651892752167285 1105 12-13 -0.5760539602619232 1105 14-15 -0.7790144389496731 1105 16-17 -0.8013650419946572 1105 18-19 -0.8303221715183824 1105 20-21 -0.8283014343350033 1105 22-23 -0.7840781107893307 1105 24-25 -0.8194644010776173 1105 26-27 -0.767282531327524 1105 28-29 -0.8673892661927312 1105 30-31 -0.9040578348292172 1105 32-33 -0.9010846711594667 1105 34-35 -0.8819081697375992 1105 36-37 -0.8692200508612302 1105 38-39 -0.8046458156333998 1105 40-41 -0.9658263969580574 1105 42-43 -0.9912036597686011 1105 44-45 -1.0464123822052898 1105 46-47 -1.125348204040641 1105 48-49 -1.1119307309715722 1105 50-51 -1.015877478996579 1105 52-53 -0.9021691935307885 1105 54-55 -0.7193782905399573 1105 56-57 -0.7096764048292528 1105 58-59 -0.6944619731100516 1105 60-61 -0.7154763707691529 1105 62-63 -0.8006822821282285 1105 64-65 -0.7402791999205647 1105 66-67 -0.7146890320688541 1105 68-69 -0.7806289134152067 1105 70-71 -0.6189200934555998 1105 72-73 -0.662329327007896 1105 74-75 -0.7791391323738281 1105 76-77 -0.7387546443906672 1105 78-79 -0.782421851843818 1105 80-81 -0.7862067871550593 1105 82-83 -0.8441821150977269 1105 84-85 -0.8521741172563289 1105 86-87 -0.925865852719209 1105 88-89 -0.9280176238205122 1105 90-91 -1.0563411612346307 1105 92-93 -1.0124509551513405 1105 94-95 -1.1464264152975439 1105 96-97 -1.070423923732701 1105 98-99 -1.1156984044525764 1105 100 -1.076635469409677 1106 1 -0.566725569677331 1106 2 -0.5567921943403249 1106 3 -0.6388051286897927 1106 4 -0.4863271350170848 1106 5 -0.5012143279448651 1106 6 -0.5154047339484293 1106 7 -0.5344214507017426 1106 8 -0.539269094944558 1106 9 -0.6904177996105219 1106 10-11 -0.6788332814889344 1106 12-13 -0.7495589539598484 1106 14-15 -0.9212812618441291 1106 16-17 -0.9528218562759179 1106 18-19 -0.9729536079007346 1106 20-21 -0.8430848248682281 1106 22-23 -0.9417488000591803 1106 24-25 -0.9026992886052483 1106 26-27 -0.9135287111006463 1106 28-29 -0.9632639212675898 1106 30-31 -1.0357698867503586 1106 32-33 -1.1132762020327363 1106 34-35 -1.0737201726817958 1106 36-37 -1.0829554297160797 1106 38-39 -1.2008735737105312 1106 40-41 -1.1817665667000128 1106 42-43 -1.164982617781071 1106 44-45 -1.0974288302301218 1106 46-47 -1.1198402809077308 1106 48-49 -1.2790307215106793 1106 50-51 -1.3057219021581048 1106 52-53 -1.2513926702647638 1106 54-55 -1.0627740838345971 1106 56-57 -0.9649099045399367 1106 58-59 -1.007105085584712 1106 60-61 -0.978255428309609 1106 62-63 -1.0182150554485574 1106 64-65 -1.0280833977061192 1106 66-67 -1.0696340153909532 1106 68-69 -1.0868124790980218 1106 70-71 -1.0476232355722246 1106 72-73 -1.011381081069807 1106 74-75 -1.0361034844654853 1106 76-77 -1.0384670134240537 1106 78-79 -0.9937616641704352 1106 80-81 -0.9441193113996356 1106 82-83 -0.9769932544275655 1106 84-85 -0.9461573716313403 1106 86-87 -1.04083567376723 1106 88-89 -1.1204706722709687 1106 90-91 -1.1330374452661935 1106 92-93 -1.2234517656403767 1106 94-95 -1.2162981045367296 1106 96-97 -1.2280783638697308 1106 98-99 -1.1481678118937637 1106 100 -1.2759448894632044 1107 1 -0.6417860371136292 1107 2 -0.6356932855160125 1107 3 -0.6022925054324695 1107 4 -0.5643956586408123 1107 5 -0.4465181213663456 1107 6 -0.4465516986923532 1107 7 -0.4675935933329214 1107 8 -0.5284016948016159 1107 9 -0.7295647107915997 1107 10-11 -0.6498416356197154 1107 12-13 -0.7382714202110705 1107 14-15 -0.8795341253643159 1107 16-17 -0.8472871400167463 1107 18-19 -0.8998795689772834 1107 20-21 -0.900743726595465 1107 22-23 -0.9064471993668803 1107 24-25 -0.8905383456312777 1107 26-27 -0.8815391357703604 1107 28-29 -1.0227401937618836 1107 30-31 -0.980516124378596 1107 32-33 -1.0112240753365072 1107 34-35 -1.029487719955192 1107 36-37 -1.0429977108909156 1107 38-39 -0.9826700717044403 1107 40-41 -1.0488154545176442 1107 42-43 -0.9595551946061036 1107 44-45 -0.9620104792628936 1107 46-47 -0.9932803047431946 1107 48-49 -1.0019972999660283 1107 50-51 -1.0431794912607444 1107 52-53 -0.9038929673711991 1107 54-55 -0.8012855220138562 1107 56-57 -0.8193060754656898 1107 58-59 -0.8338210635502961 1107 60-61 -0.8676099792269554 1107 62-63 -0.8074854122608386 1107 64-65 -0.7917861138447719 1107 66-67 -0.7510431033327833 1107 68-69 -0.8083201394225128 1107 70-71 -0.8397379471111748 1107 72-73 -0.7206742355701721 1107 74-75 -0.7785768160245468 1107 76-77 -0.7546741275748552 1107 78-79 -0.8091624900426311 1107 80-81 -0.8552751140138355 1107 82-83 -0.7671853944140743 1107 84-85 -0.7766671895343897 1107 86-87 -0.801181589348829 1107 88-89 -0.845956211138347 1107 90-91 -0.9117384289814154 1107 92-93 -0.9876126115316879 1107 94-95 -1.0244315396394832 1107 96-97 -1.025576727692041 1107 98-99 -1.028181420205378 1107 100 -1.0570745299727875 1108 1 -0.5166139849164502 1108 2 -0.535160813921717 1108 3 -0.5290912514112627 1108 4 -0.36516171910245276 1108 5 -0.40259051800639867 1108 6 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56-57 1.2027310852976214 2311 58-59 1.168988767745816 2311 60-61 1.1784750386217127 2311 62-63 1.148796166124761 2311 64-65 1.1441372248860802 2311 66-67 1.1499528872434155 2311 68-69 1.1603398959880877 2311 70-71 1.1226411609967073 2311 72-73 1.1585739659518879 2311 74-75 1.1847688592543761 2311 76-77 1.2084666713149232 2311 78-79 1.2163868611394264 2311 80-81 1.1512737338949997 2311 82-83 1.177731823167985 2311 84-85 1.20865916160459 2311 86-87 1.1482979045187278 2311 88-89 1.2297246310803018 2311 90-91 1.3184868401547796 2311 92-93 1.3571253781709522 2311 94-95 1.374089421696862 2311 96-97 1.3638103442677156 2311 98-99 1.4376239907920763 2311 100 1.5491244717093622 2312 1 0.6522206008283256 2312 2 0.626222832584947 2312 3 0.6151849830977518 2312 4 0.35643661620976275 2312 5 0.4876385614066834 2312 6 0.5014053344493519 2312 7 0.4317779189176747 2312 8 0.5720998162785165 2312 9 0.7382005727252121 2312 10-11 0.6396046803582536 2312 12-13 0.7908600425912411 2312 14-15 1.0102382697812473 2312 16-17 1.0214353718106395 2312 18-19 1.0049746616138364 2312 20-21 1.0044546214974872 2312 22-23 1.0577308350722419 2312 24-25 1.001043075063258 2312 26-27 1.028082317785163 2312 28-29 1.102819837030502 2312 30-31 1.123418398737499 2312 32-33 1.157480184970062 2312 34-35 1.1280406885300707 2312 36-37 1.1486482571940968 2312 38-39 1.1526316567434662 2312 40-41 1.2761702791749272 2312 42-43 1.2478924505557245 2312 44-45 1.308050397192467 2312 46-47 1.3315524160111494 2312 48-49 1.3220277600459909 2312 50-51 1.3316293869831881 2312 52-53 1.2043029832668921 2312 54-55 1.175707143130424 2312 56-57 1.0753079516326665 2312 58-59 1.0870821886726532 2312 60-61 1.0825306482594996 2312 62-63 1.0525602963142262 2312 64-65 1.0701123097712326 2312 66-67 1.0014996966210603 2312 68-69 1.0523798593533868 2312 70-71 1.0201525478901559 2312 72-73 1.1027602770241316 2312 74-75 1.121333556217074 2312 76-77 1.1034580665261444 2312 78-79 1.0971553282143134 2312 80-81 1.1431211109609798 2312 82-83 1.0997630063222026 2312 84-85 1.139398068661368 2312 86-87 1.1604679423903121 2312 88-89 1.1534376444397196 2312 90-91 1.2046042124441492 2312 92-93 1.2532085789928686 2312 94-95 1.3241349044925066 2312 96-97 1.3202855067479895 2312 98-99 1.0243306189901418 2312 100 1.2833045322456442 2313 1 0.6140904502423297 2313 2 0.6261506434501847 2313 3 0.6256807139429661 2313 4 0.5136617264569168 2313 5 0.393286717730021 2313 6 0.5042456653716485 2313 7 0.6033242685156281 2313 8 0.5742258829790643 2313 9 0.7439903884088395 2313 10-11 0.7622812470056246 2313 12-13 0.8629196988328189 2313 14-15 0.9929384981363185 2313 16-17 1.0265947948007295 2313 18-19 0.9949358976598361 2313 20-21 0.9564574300845265 2313 22-23 0.9739150332122577 2313 24-25 0.9792861131275217 2313 26-27 0.9829514754618245 2313 28-29 1.0384636745743947 2313 30-31 1.0710273006933875 2313 32-33 1.1114907484963865 2313 34-35 1.1236477164903889 2313 36-37 1.1934493874610936 2313 38-39 1.1958487189118827 2313 40-41 1.2067870028772703 2313 42-43 1.212598817329102 2313 44-45 1.3048970103527395 2313 46-47 1.3276403073999319 2313 48-49 1.282047000169193 2313 50-51 1.3028646594194484 2313 52-53 1.194990439860085 2313 54-55 1.0630011582289853 2313 56-57 1.0813473676951872 2313 58-59 1.140046314830002 2313 60-61 1.0695857828508224 2313 62-63 1.0605010581680645 2313 64-65 1.0460356488203644 2313 66-67 1.0365810271884186 2313 68-69 1.0885471207622004 2313 70-71 1.04893093982583 2313 72-73 1.044739414772394 2313 74-75 1.0121515451607692 2313 76-77 0.8065423451602669 2313 78-79 1.0082096719049147 2313 80-81 1.0685489774129344 2313 82-83 1.0798815170332823 2313 84-85 1.0926701115572648 2313 86-87 1.166365612078856 2313 88-89 1.1305496925366754 2313 90-91 1.2200154325328008 2313 92-93 1.2727457089211 2313 94-95 1.321951038143208 2313 96-97 1.3670341698873933 2313 98-99 1.317032530576693 2313 100 1.4134921796297775 2314 1 0.5662451676253575 2314 2 0.5246587266556588 2314 3 0.5706150119090871 2314 4 0.4733087873881914 2314 5 0.46624422697779977 2314 6 0.4529489799248978 2314 7 0.468383583725263 2314 8 0.54169334869664 2314 9 0.6678509282369944 2314 10-11 0.613773262068527 2314 12-13 0.7174166213980797 2314 14-15 0.8487089737710178 2314 16-17 0.8656629140713221 2314 18-19 0.7989605703061287 2314 20-21 0.8657211119190578 2314 22-23 0.8972994989297476 2314 24-25 0.9115013998316286 2314 26-27 0.9748806311158731 2314 28-29 0.9758589463384055 2314 30-31 0.992174179817809 2314 32-33 1.0040899745304657 2314 34-35 1.0109014890239578 2314 36-37 0.9783023020351322 2314 38-39 1.0022760017981298 2314 40-41 1.0251359732273642 2314 42-43 1.1435028210767868 2314 44-45 1.1376403851397399 2314 46-47 1.1119370487872544 2314 48-49 1.032316033141278 2314 50-51 1.0016198027865997 2314 52-53 1.0068170273206434 2314 54-55 0.9206794007318422 2314 56-57 0.8470198610632025 2314 58-59 0.8282432652331408 2314 60-61 0.8701619179664988 2314 62-63 0.8988455807877003 2314 64-65 0.8843437759342194 2314 66-67 0.8971129887006484 2314 68-69 0.8885370177380452 2314 70-71 0.8870259279893489 2314 72-73 0.924367395293828 2314 74-75 0.9315580757092548 2314 76-77 0.9114471642665904 2314 78-79 0.9060231734924784 2314 80-81 0.9370040652036273 2314 82-83 0.9674504673026938 2314 84-85 1.051824113463713 2314 86-87 1.077666583682145 2314 88-89 1.13476746979606 2314 90-91 1.1450417043263492 2314 92-93 1.2010751275868614 2314 94-95 1.1501929296775906 2314 96-97 1.2438124293710366 2314 98-99 1.2955477882016346 2314 100 1.2925825855489528 2315 1 0.40101946980256997 2315 2 0.37378928053882277 2315 3 0.4249103457395691 2315 4 0.19451531263361943 2315 5 0.274548654412456 2315 6 0.3577429241826593 2315 7 0.3260877131331412 2315 8 0.3514707689235408 2315 9 0.5649310461155963 2315 10-11 0.46549689871354616 2315 12-13 0.4840381117148169 2315 14-15 0.6793994879129173 2315 16-17 0.7359418848341406 2315 18-19 0.5474624372179733 2315 20-21 0.6956329706945894 2315 22-23 0.5248311593777188 2315 24-25 0.6784960358066812 2315 26-27 0.7864748866562152 2315 28-29 0.777904501207594 2315 30-31 0.8411920513032243 2315 32-33 0.8846467961701947 2315 34-35 0.8679414003864565 2315 36-37 0.9617670478308185 2315 38-39 1.0255755872770962 2315 40-41 1.0500297636395786 2315 42-43 1.1266687809855327 2315 44-45 1.1141821627610895 2315 46-47 1.1057980823733828 2315 48-49 1.1960477199049464 2315 50-51 1.0163081431091499 2315 52-53 1.0340885754927243 2315 54-55 0.9656113537867554 2315 56-57 0.9125011083614396 2315 58-59 0.9068025271140527 2315 60-61 0.9102551803018955 2315 62-63 0.9227600424001068 2315 64-65 0.9572499402694703 2315 66-67 0.9127018495252699 2315 68-69 0.9267863454800036 2315 70-71 0.8304968707047351 2315 72-73 0.608126511099826 2315 74-75 0.8511240660475394 2315 76-77 0.8563246983757651 2315 78-79 0.8670068218522147 2315 80-81 0.639196690803896 2315 82-83 0.7026535257992599 2315 84-85 0.914623764568649 2315 86-87 1.0310843497742184 2315 88-89 1.0349387421431686 2315 90-91 1.0819252773305017 2315 92-93 1.141003002689871 2315 94-95 1.1713187774279596 2315 96-97 0.9815851852568045 2315 98-99 0.4762132191846682 2315 100 1.006743787738312 2316 1 0.3089025831995258 2316 2 0.28337548105313815 2316 3 0.31215078772057225 2316 4 0.18496630273546089 2316 5 0.1131092049438891 2316 6 0.17199461139659888 2316 7 0.08850600817883247 2316 8 0.19640514347155857 2316 9 0.28547237029356154 2316 10-11 0.2736217715443203 2316 12-13 0.0717310923001051 2316 14-15 0.13487807629155668 2316 16-17 0.29425835930673117 2316 18-19 0.3481349836996017 2316 20-21 0.3667717246853499 2316 22-23 0.3124764183044917 2316 24-25 0.36936731255716637 2316 26-27 0.42364359804068386 2316 28-29 0.3910075182950479 2316 30-31 0.3232894682458891 2316 32-33 0.5393152859022692 2316 34-35 0.48618783065931126 2316 36-37 0.49824940287015806 2316 38-39 0.659713406446329 2316 40-41 0.7535625146706195 2316 42-43 0.7140638689233114 2316 44-45 0.6500219301425219 2316 46-47 0.4535253588149857 2316 48-49 0.4169697397323162 2316 50-51 0.479892355289131 2316 52-53 0.5538507710730656 2316 54-55 0.2573692503320757 2316 56-57 0.12125115841197953 2316 58-59 0.22227386429290164 2316 60-61 0.32149069424536947 2316 62-63 0.43488709437013284 2316 64-65 0.39337021539628125 2316 66-67 0.379753190348616 2316 68-69 0.3508595422509373 2316 70-71 0.12793388975990183 2316 72-73 0.03359282381723716 2316 74-75 0.19790611248954093 2316 76-77 0.34277318758486786 2316 78-79 0.4101910719888835 2316 80-81 0.5559763286912904 2316 82-83 0.5690569164067618 2316 84-85 0.647090360429118 2316 86-87 0.7137990431012788 2316 88-89 0.695727774619229 2316 90-91 0.5895935002625592 2316 92-93 0.5556337716786359 2316 94-95 0.6918718073038406 2316 96-97 0.6752816362853089 2316 98-99 0.515086044233307 2316 100 0.42479581251762255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 29.0 1 503.0 2 205.0 3 181.0 4 134.0 5 131.0 6 129.0 7 541.0 8 1999.0 9 3675.0 10 6067.0 11 9583.0 12 13927.0 13 17903.0 14 21234.0 15 24820.0 16 29354.0 17 35436.0 18 42549.0 19 51402.0 20 62233.0 21 75309.0 22 84009.0 23 86525.0 24 86432.0 25 88640.0 26 98222.0 27 111580.0 28 131415.0 29 157435.0 30 190698.0 31 237733.0 32 301456.0 33 398555.0 34 569938.0 35 793158.0 36 1190092.0 37 1652834.0 38 1343949.0 39 359739.0 40 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06570709882988 19.706027219012707 12.83343435573807 23.39483132641934 2 15.898285895476642 19.32915544970498 41.01357354652544 23.758985108292936 3 19.567670535256998 25.535486505029816 28.457596899973908 26.43924605973928 4 12.908772126906934 15.699632730639543 37.898042739982834 33.49355240247069 5 13.56298399128627 37.64371235170344 34.08065709085625 14.712646566154037 6 31.23994488886784 36.60563132141289 17.64832263164598 14.506101158073296 7 28.186797656576367 31.745786800460117 22.022700723108674 18.04471481985484 8 26.763491944961405 35.26670934460843 19.32584560203327 18.643953108396897 9 26.721374956579524 15.643248304713575 20.80344343255856 36.83193330614834 10-11 26.333972312307274 25.80097903209788 26.756251792673112 21.108796862921732 12-13 26.200666488374207 23.28363959704294 27.544293663745055 22.971400250837792 14-15 23.493507932065363 24.550150287123724 25.318370373716842 26.637971407094074 16-17 22.5061687720467 27.428400419515757 26.37031596856591 23.695114839871636 18-19 22.08609566108062 27.127430997899936 28.61099630666913 22.175477034350312 20-21 23.28851897598821 26.36620773172993 28.24354858680454 22.10172470547732 22-23 23.563072141080237 25.989616255684982 28.021214465000995 22.426097138233786 24-25 22.971986390122638 25.973451062843573 28.572606398062998 22.481956148970788 26-27 22.86888386593017 26.26270892845408 27.86072893997317 23.007678265642575 28-29 22.313570749444736 26.738768391188295 28.249291627977037 22.698369231389933 30-31 23.16805247602394 26.343626686199578 28.173013414222275 22.315307423554202 32-33 23.28647670614837 26.47609730959096 27.59832972811785 22.639096256142825 34-35 23.264591570595933 26.414792792295437 28.120881242866307 22.19973439424232 36-37 22.94941629681816 26.208703823599066 27.84860060365655 22.993279275926227 38-39 23.162328818917878 26.379674282714333 28.086701468020546 22.371295430347242 40-41 22.948254380922428 25.615631049799596 28.54957867678774 22.886535892490233 42-43 22.825807577896047 25.915644568773804 27.962937178469183 23.295610674860967 44-45 22.856106879290795 26.550228360017975 27.926145722332752 22.667519038358478 46-47 22.856205730349956 26.351396948534607 27.38675720737286 23.405640113742578 48-49 23.376274251602776 26.295848319642523 27.38026327092577 22.947614157828927 50-51 23.125035335663434 26.26931139308769 27.41176473430722 23.193888536941653 52-53 22.825329200297123 26.716005028076328 27.108001909934416 23.35066386169214 54-55 22.632642669804504 26.558393667398388 27.308100038896754 23.500863623900354 56-57 23.32022911371904 26.291431746894546 26.88409679503091 23.5042423443555 58-59 22.693527420072186 26.618362466844125 27.452682839245924 23.235427273837765 60-61 22.88073739385733 26.976138442911058 27.02871072164029 23.114413441591317 62-63 23.104389648841263 26.537472783689136 26.80119322612773 23.55694434134187 64-65 22.57657585937777 26.536051823932528 27.099893010970167 23.787479305719536 66-67 22.93931000472252 26.281166183279915 27.001510505582637 23.77801330641493 68-69 22.520084467488257 26.426361463146236 27.207660357379922 23.845893711985582 70-71 22.618457176624986 26.153049476462975 27.268919203502172 23.959574143409863 72-73 23.077034608701076 26.36731035559361 27.201246006999696 23.35440902870562 74-75 23.245387790880006 26.580484069945054 26.921655142687463 23.252472996487477 76-77 23.05129970932617 26.4491080409613 26.64317446795865 23.856417781753876 78-79 22.901392605975033 26.488685827654752 26.784093134899674 23.825828431470548 80-81 22.64902320679642 26.844405524712112 27.079184286650676 23.427386981840794 82-83 23.342634912407295 26.21337227671212 27.759188779735943 22.684804031144637 84-85 23.42459272264942 26.57355318914043 26.82863224335494 23.173221844855213 86-87 22.596825709344557 27.094517570812155 27.106795835053326 23.20186088478996 88-89 23.168409652164712 26.146389038808405 27.104785447053555 23.580415861973332 90-91 23.15823042651455 26.217147254168943 27.100973657404626 23.523648661911878 92-93 23.38674490705833 26.398135489171963 26.989824625912824 23.22529497785688 94-95 22.633294958849103 26.381112988897172 27.422635952583153 23.562956099670576 96-97 22.861947509232014 26.292744530102663 27.08436795458414 23.76094000608119 98-99 22.452606846194886 26.714917692322608 26.884184672613088 23.948290788869418 100 23.05523350317645 26.345277922847327 26.34648599679208 24.253002577184148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1943.0 1 1362.0 2 1658.0 3 2373.5 4 1729.0 5 1369.0 6 2037.5 7 3661.5 8 5768.5 9 6699.5 10 5805.0 11 4929.0 12 5140.0 13 5859.5 14 6737.0 15 7619.0 16 8558.5 17 9520.5 18 10255.5 19 10903.5 20 11706.5 21 12427.0 22 13575.5 23 15520.0 24 17898.0 25 20734.5 26 24477.5 27 29156.0 28 35598.5 29 47483.0 30 64066.0 31 79913.0 32 101002.5 33 124105.5 34 142335.5 35 161030.5 36 180018.5 37 209774.5 38 225975.5 39 221707.5 40 216722.5 41 210692.5 42 214081.5 43 227763.5 44 247138.5 45 281987.5 46 318217.5 47 346193.0 48 366835.0 49 371645.5 50 368462.5 51 379286.5 52 381359.5 53 364540.5 54 346615.5 55 310595.5 56 276199.0 57 236053.0 58 196386.0 59 178886.0 60 149187.5 61 112057.0 62 87216.5 63 70896.5 64 53179.5 65 33733.5 66 23019.5 67 16807.0 68 12786.5 69 9648.5 70 7481.0 71 5801.5 72 4417.0 73 3431.5 74 2713.5 75 1974.5 76 1341.5 77 879.0 78 582.0 79 405.5 80 293.0 81 216.5 82 163.5 83 135.5 84 103.0 85 71.5 86 70.0 87 60.5 88 47.0 89 42.5 90 32.0 91 24.5 92 24.5 93 20.5 94 12.0 95 6.0 96 6.0 97 6.0 98 5.0 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055953937337145716 2 0.1319561233208837 3 0.016727002635861608 4 0.01642507118034064 5 0.019275304120458574 6 0.052934622781936044 7 0.05775344881205069 8 0.04935975434856779 9 0.0410626779508516 10-11 0.03960132970613012 12-13 0.040597703509349305 14-15 0.01857482314364993 16-17 0.017300672401351445 18-19 0.0175663720822099 20-21 0.021739064797509672 22-23 0.02635861606698047 24-25 0.024957654113363183 26-27 0.019239072345796058 28-29 0.01855066862720825 30-31 0.016292221339911414 32-33 0.02381031458238351 34-35 0.023749928291279313 36-37 0.023834469098825185 38-39 0.021775296572172188 40-41 0.025561517024405123 42-43 0.03294072179733756 44-45 0.04239721498425427 46-47 0.0479285992493984 48-49 0.039903261161651084 50-51 0.02740329890308302 52-53 0.039867029386988564 54-55 0.036044577160093114 56-57 0.03833925622205247 58-59 0.04333320249636927 60-61 0.022922636103151865 62-63 0.025730598639496862 64-65 0.025042194920909054 66-67 0.03217381590031431 68-69 0.03902162131152986 70-71 0.04036219697404295 72-73 0.04428730589581553 74-75 0.026726972442716056 76-77 0.020217330261683993 78-79 0.019619505979752476 80-81 0.024383984347873346 82-83 0.020307909698340284 84-85 0.012355035159917994 86-87 0.014100198972829188 88-89 0.018224582655245604 90-91 0.020561532120977895 92-93 0.019408153960887797 94-95 0.02361103982173967 96-97 0.024589297737627604 98-99 0.027457646565076797 100 0.02881633811492115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8280025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.23674876081103 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9422696090545 45.1427866600558 2 14.292192723541646 17.218304446581396 3 4.971466338324979 8.983949063835329 4 2.192468179992745 5.282686196971824 5 1.127534098621408 3.395949410895264 6 0.6351427493049362 2.2955360530279334 7 0.4397843578162813 1.8543825909499767 8 0.2950404098632775 1.421782003457677 9 0.19695832044713227 1.0677715978612694 >10 0.8571606840904398 8.652496569938632 >50 0.035023130145060886 1.4518279644913166 >100 0.013707108941045935 1.4429891761198173 >500 7.566103475610502E-4 0.29975940962061276 >1k 3.913259282650184E-4 0.44581319174040596 >5k 2.6088395190362187E-5 0.08100979949804452 >10k+ 7.826518557108656E-5 0.962955864954738 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26964 0.32565119066669485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20858 0.2519074519702537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13455 0.16249950936138477 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.2077258220838705E-5 0.0 4 0.0 0.0 0.0 1.2077258220838705E-5 1.2077258220838705E-5 5 0.0 0.0 0.0 1.2077258220838705E-5 2.415451644167741E-5 6 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 2.415451644167741E-5 7 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 2.415451644167741E-5 8 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 3.623177466251611E-5 9 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 3.623177466251611E-5 10-11 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 1.0869532398754835E-4 12-13 1.2077258220838705E-4 0.0 0.0 1.2077258220838705E-5 1.388884695396451E-4 14-15 1.2077258220838705E-4 0.0 0.0 1.8115887331258057E-5 1.6908161509174188E-4 16-17 2.294679061959354E-4 0.0 0.0 8.454080754587094E-5 2.2342927708551605E-4 18-19 2.294679061959354E-4 0.0 0.0 1.1473395309796771E-4 2.3550653530635476E-4 20-21 2.294679061959354E-4 0.0 0.0 1.6908161509174188E-4 2.415451644167741E-4 22-23 2.3550653530635476E-4 0.0 0.0 3.562791175147418E-4 2.596610517480322E-4 24-25 2.415451644167741E-4 0.0 0.0 7.789831552440965E-4 2.898541973001289E-4 26-27 2.536224226376128E-4 0.0 0.0 0.001455309615611064 3.1400871374180633E-4 28-29 2.536224226376128E-4 0.0 0.0 0.004178731344410192 3.200473428522257E-4 30-31 2.536224226376128E-4 0.0 0.0 0.014631598334546091 3.5024048840432245E-4 32-33 2.536224226376128E-4 0.0 0.0 0.03940205494548628 3.562791175147418E-4 34-35 2.536224226376128E-4 0.0 0.0 0.07509035298806466 3.7439500484599987E-4 36-37 2.536224226376128E-4 0.0 0.0 0.13506601731274967 3.7439500484599987E-4 38-39 2.536224226376128E-4 0.0 0.0 0.22701622277710515 3.7439500484599987E-4 40-41 2.536224226376128E-4 0.0 0.0 0.32677437568123285 3.804336339564192E-4 42-43 2.536224226376128E-4 0.0 0.0 0.4231569349126361 3.925108921772579E-4 44-45 2.536224226376128E-4 0.0 0.0 0.5334223024688935 3.9854952128767724E-4 46-47 2.536224226376128E-4 0.0 0.0 0.6622141841359175 3.9854952128767724E-4 48-49 2.536224226376128E-4 0.0 0.0 0.8069722011708902 3.9854952128767724E-4 50-51 2.596610517480322E-4 0.0 0.0 0.9569959027901486 3.9854952128767724E-4 52-53 2.6569968085845153E-4 0.0 0.0 1.1039036717884305 4.2270403772935467E-4 54-55 2.6569968085845153E-4 0.0 0.0 1.262641115214024 4.649744415022902E-4 56-57 2.6569968085845153E-4 0.0 0.0 1.4476284793826104 4.710130706127095E-4 58-59 2.6569968085845153E-4 0.0 0.0 1.6600251810833904 4.7705169972312887E-4 60-61 2.9589282641054825E-4 0.0 0.0 1.871582513337822 4.891289579439676E-4 62-63 3.5024048840432245E-4 0.0 0.0 2.0892448996228876 5.072448452752256E-4 64-65 3.5024048840432245E-4 0.0 0.0 2.312631906304631 5.253607326064836E-4 66-67 3.7439500484599987E-4 0.0 0.0 2.5569488014830872 5.313993617169031E-4 68-69 3.7439500484599987E-4 0.0 0.0 2.830202807358673 5.313993617169031E-4 70-71 3.804336339564192E-4 0.0 0.0 3.1218806706501487 5.313993617169031E-4 72-73 4.2270403772935467E-4 0.0 0.0 3.4089933303341473 5.797083946002578E-4 74-75 4.2270403772935467E-4 0.0 0.0 3.6910878892273873 5.797083946002578E-4 76-77 4.28742666839774E-4 0.0 0.0 4.011292236436484 5.917856528210965E-4 78-79 4.347812959501934E-4 0.0 0.0 4.360892630155103 6.038629110419353E-4 80-81 4.347812959501934E-4 0.0 0.0 4.7238685873533 6.280174274836127E-4 82-83 4.347812959501934E-4 0.0 0.0 5.092840903258143 6.763264603669675E-4 84-85 4.347812959501934E-4 0.0 0.0 5.454651405038028 6.763264603669675E-4 86-87 4.649744415022902E-4 0.0 0.0 5.837120057004659 6.763264603669675E-4 88 4.710130706127095E-4 0.0 0.0 6.143194012095374 6.763264603669675E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14240 0.0 45.272213 1 GTATCAA 24980 0.0 40.350433 1 GTACTAG 6500 0.0 35.328693 1 CAACGCA 28660 0.0 33.425594 5 ATCAACG 29255 0.0 32.922318 3 GCATCGT 6630 0.0 32.044228 8 TATCAAC 31660 0.0 31.673326 2 AACGCAG 32370 0.0 30.306158 6 TACCTGG 5690 0.0 30.020332 2 GTGGTAT 4680 0.0 29.96343 1 TCAACGC 33325 0.0 29.169497 4 TAGGCAT 8380 0.0 28.495144 5 GTACATG 34520 0.0 28.394852 1 TACATGG 35290 0.0 27.401937 2 ACGCAGA 34920 0.0 27.299047 7 CGCAGAG 35625 0.0 26.783424 8 CTAGGCA 9025 0.0 26.562656 4 CTGTGCG 9400 0.0 26.551657 9 TAGTACT 9555 0.0 26.417524 4 ACATGGG 35230 0.0 26.364702 3 >>END_MODULE