FastQCFastQC Report
Mon 6 Jun 2016
ERR1211203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280025
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248110.29964885371722916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205600.24830842902044378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129360.1562314123447695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152400.046.642341
GTATCAA258150.037.8066981
GTACTAG64150.032.685241
TACCTGG55900.032.1149832
CAACGCA296150.031.4782055
ATCAACG296550.031.3969363
TATCAAC327750.029.5666472
CTAACGC29150.028.7000163
AACGCAG333450.028.5206746
GTGGTAT52050.027.8191281
TCAACGC339900.027.6830314
TAGGCAT82700.027.6696385
CTGTGCG90250.027.6456839
GGGCACC80200.027.37330294
TCTAACG30400.027.3625012
GTACATG343000.027.1932471
TACATGG344050.026.7862632
TAGTACT92550.026.5598724
ACCTGGG66400.026.4730643
TAACGCC32100.026.0624774
Produced by FastQC (version 0.11.5)