##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8280025 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15604228248103 33.0 31.0 34.0 30.0 34.0 2 32.29936310578773 34.0 31.0 34.0 30.0 34.0 3 32.37804197451095 34.0 31.0 34.0 30.0 34.0 4 35.99184169129924 37.0 35.0 37.0 35.0 37.0 5 35.873825260189435 37.0 35.0 37.0 35.0 37.0 6 35.90218133399355 37.0 35.0 37.0 35.0 37.0 7 35.840068840371856 37.0 35.0 37.0 35.0 37.0 8 35.82820100180857 37.0 35.0 37.0 35.0 37.0 9 37.43464228670807 39.0 37.0 39.0 34.0 39.0 10-11 37.41182611163613 39.0 37.0 39.0 34.0 39.0 12-13 37.301289851177984 39.0 37.0 39.0 34.0 39.0 14-15 38.52223362852165 40.0 38.0 41.0 34.0 41.0 16-17 38.472309443510134 40.0 38.0 41.0 34.0 41.0 18-19 38.48866609702266 40.0 38.0 41.0 34.0 41.0 20-21 38.44919109543751 40.0 38.0 41.0 34.0 41.0 22-23 38.31640121859536 40.0 38.0 41.0 34.0 41.0 24-25 38.24438549400515 40.0 38.0 41.0 34.0 41.0 26-27 38.1033506541345 40.0 37.0 41.0 33.0 41.0 28-29 38.08117276215953 40.0 37.0 41.0 33.0 41.0 30-31 38.09700659116368 40.0 37.0 41.0 33.5 41.0 32-33 37.967767911328764 40.0 37.0 41.0 33.0 41.0 34-35 37.83147901123487 40.0 37.0 41.0 33.0 41.0 36-37 37.69015063360316 40.0 37.0 41.0 33.0 41.0 38-39 37.59683702645825 40.0 36.5 41.0 33.0 41.0 40-41 37.490712588428174 40.0 36.0 41.0 32.0 41.0 42-43 37.34344219975181 40.0 36.0 41.0 32.0 41.0 44-45 37.18931428347136 39.0 36.0 41.0 31.5 41.0 46-47 36.940545771299 39.0 35.5 41.0 31.0 41.0 48-49 36.693787096053455 39.0 35.0 41.0 30.5 41.0 50-51 36.478831646039716 39.0 35.0 40.5 30.0 41.0 52-53 36.25915978514558 38.5 35.0 40.0 30.0 41.0 54-55 36.003799867754026 38.0 35.0 40.0 29.0 41.0 56-57 35.72727805773533 38.0 34.5 40.0 28.5 41.0 58-59 35.43101439911111 37.5 34.0 40.0 28.0 41.0 60-61 35.15371016391859 37.0 34.0 40.0 28.0 41.0 62-63 34.83234929846226 36.0 34.0 39.5 27.5 41.0 64-65 34.4485537181349 36.0 33.5 39.0 26.5 40.5 66-67 34.13859155014629 35.0 33.0 39.0 26.0 40.0 68-69 33.81127726063629 35.0 33.0 38.0 26.0 40.0 70-71 33.50466840377897 35.0 33.0 37.5 26.0 40.0 72-73 33.177667821051266 35.0 32.0 37.0 26.0 39.5 74-75 32.855215171451775 35.0 32.0 36.5 25.0 39.0 76-77 31.89625882772093 34.0 30.5 35.0 24.5 38.0 78-79 32.4090398277783 34.5 32.0 35.5 25.0 37.5 80-81 32.362592564635975 35.0 32.0 35.0 25.0 37.0 82-83 32.136941977832194 34.5 32.0 35.0 25.0 37.0 84-85 31.907738201273546 34.0 31.5 35.0 25.0 36.0 86-87 31.689080830069955 34.0 31.0 35.0 24.0 36.0 88-89 31.49443818104414 34.0 31.0 35.0 24.0 36.0 90-91 31.295379603322452 34.0 31.0 35.0 23.5 35.0 92-93 31.119767029688923 34.0 31.0 35.0 23.0 35.0 94-95 30.939873792651593 34.0 30.5 35.0 22.5 35.0 96-97 30.775477670659207 34.0 30.0 35.0 20.0 35.0 98-99 30.608863680967147 34.0 30.0 35.0 20.0 35.0 100 28.791211379192696 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34122902993011195 1101 2 -0.3333705236858755 1101 3 -0.3779123462904437 1101 4 -0.18111957235096554 1101 5 -0.24440871228830474 1101 6 -0.31472972305108016 1101 7 -0.3177430415929976 1101 8 -0.40047674286510215 1101 9 -0.5075030154421256 1101 10-11 -0.5170920584147254 1101 12-13 -0.48579002256546033 1101 14-15 -0.6365601548532993 1101 16-17 -0.6569005551568878 1101 18-19 -0.6473354247730967 1101 20-21 -0.6757011600264775 1101 22-23 -0.8002262393405815 1101 24-25 -0.7046332209411901 1101 26-27 -0.8034439629916008 1101 28-29 -0.9082777517874732 1101 30-31 -0.6878835903339464 1101 32-33 -0.655739550947608 1101 34-35 -0.6477778575943773 1101 36-37 -0.6645450906225889 1101 38-39 -0.7271268726413851 1101 40-41 -0.7598687148653909 1101 42-43 -0.7240974608555106 1101 44-45 -0.7098420390639504 1101 46-47 -0.7491814877534182 1101 48-49 -0.7290255534220975 1101 50-51 -0.6863895285076893 1101 52-53 -0.7285706782461361 1101 54-55 -0.6964796467039349 1101 56-57 -0.8674983226073962 1101 58-59 -0.7133624054257695 1101 60-61 -0.8243576467045841 1101 62-63 -0.8671115308766346 1101 64-65 -0.8416483104209362 1101 66-67 -0.8269182908137367 1101 68-69 -0.9169356564399678 1101 70-71 -1.0101669969084028 1101 72-73 -1.0430707235711765 1101 74-75 -1.0155908375159157 1101 76-77 -1.088029398676646 1101 78-79 -0.9289892538152529 1101 80-81 -0.8896983693814526 1101 82-83 -0.7979029230914421 1101 84-85 -0.8189638806091999 1101 86-87 -1.0248329627536314 1101 88-89 -1.0663498258578628 1101 90-91 -1.001660586176449 1101 92-93 -1.0671280670096799 1101 94-95 -1.0160246471261303 1101 96-97 -1.1108958498012136 1101 98-99 -1.1515132022281769 1101 100 -1.0654927539268364 1102 1 -0.37640329490256974 1102 2 -0.3447534326196582 1102 3 -0.3844935550312911 1102 4 -0.2759258817600454 1102 5 -0.33206373092640007 1102 6 -0.3760950679398647 1102 7 -0.3095443010700052 1102 8 -0.3338317503900683 1102 9 -0.4472929888815784 1102 10-11 -0.5260754008102495 1102 12-13 -0.5148810165744777 1102 14-15 -0.6287824462680476 1102 16-17 -0.7048395333087427 1102 18-19 -0.6553855375357571 1102 20-21 -0.7843338674416245 1102 22-23 -0.7736030888858565 1102 24-25 -0.7714523881255602 1102 26-27 -0.8663090255803851 1102 28-29 -0.8357243835071344 1102 30-31 -0.784137728567039 1102 32-33 -0.7771085842030274 1102 34-35 -0.8062733981818937 1102 36-37 -0.8440807091974492 1102 38-39 -0.8231084990437907 1102 40-41 -0.8224308991204268 1102 42-43 -0.8037617996168294 1102 44-45 -0.818339658187675 1102 46-47 -0.831581080790933 1102 48-49 -0.8545372252724022 1102 50-51 -0.9331683356531499 1102 52-53 -0.9303151166712027 1102 54-55 -0.9292777969888917 1102 56-57 -0.9971680523132207 1102 58-59 -0.9894922140695996 1102 60-61 -0.9883812903437672 1102 62-63 -1.0017082526192596 1102 64-65 -0.9648002888478828 1102 66-67 -1.0126432602277902 1102 68-69 -0.9999717535750179 1102 70-71 -1.0458696075664449 1102 72-73 -1.0286694819480502 1102 74-75 -1.1790578226596011 1102 76-77 -1.0017221658509463 1102 78-79 -0.8519172554741061 1102 80-81 -0.8166058652454993 1102 82-83 -0.8393059399997966 1102 84-85 -0.8851629401171124 1102 86-87 -0.9413181729333928 1102 88-89 -0.9877837160677565 1102 90-91 -1.0327599430226968 1102 92-93 -0.9948732543702583 1102 94-95 -1.1403348588006175 1102 96-97 -1.2634925046332022 1102 98-99 -1.231956105997913 1102 100 -1.274498397111234 1103 1 -0.35880602129896744 1103 2 -0.3675414609480754 1103 3 -0.37161902839131145 1103 4 -0.2244052176226603 1103 5 -0.27993412787712657 1103 6 -0.33025866544016935 1103 7 -0.41509385017708667 1103 8 -0.40391002948057775 1103 9 -0.5453032171978052 1103 10-11 -0.4982856882653479 1103 12-13 -0.5793689082535494 1103 14-15 -0.6757752096815182 1103 16-17 -0.6719628143696923 1103 18-19 -0.681037758489559 1103 20-21 -0.6756326529333805 1103 22-23 -0.7560885511882063 1103 24-25 -0.8426033419962735 1103 26-27 -0.8166472628208865 1103 28-29 -0.8458192547287311 1103 30-31 -0.7753540062074862 1103 32-33 -0.7788182254785525 1103 34-35 -0.8908765757728077 1103 36-37 -0.886505158293069 1103 38-39 -0.8675382341482631 1103 40-41 -0.9001100902774226 1103 42-43 -0.7702409916067197 1103 44-45 -0.908886947488206 1103 46-47 -0.9057711270095083 1103 48-49 -1.0041209999589213 1103 50-51 -0.9594199767169158 1103 52-53 -1.0753254375478676 1103 54-55 -1.0182227794210021 1103 56-57 -1.0105189652431719 1103 58-59 -1.0557616916494865 1103 60-61 -1.0734763140149326 1103 62-63 -0.9448392612287009 1103 64-65 -0.9977851850046164 1103 66-67 -1.1060591793035641 1103 68-69 -1.091693251542928 1103 70-71 -1.126416445219654 1103 72-73 -1.0188577702950994 1103 74-75 -1.0623772328171235 1103 76-77 -0.9951324612317656 1103 78-79 -0.845473596709077 1103 80-81 -0.7937930896846481 1103 82-83 -0.9519853196323744 1103 84-85 -1.0398767541147969 1103 86-87 -0.9462327584597219 1103 88-89 -0.9856255343030291 1103 90-91 -0.9547186242501979 1103 92-93 -1.0073869010849137 1103 94-95 -0.9923321306723203 1103 96-97 -1.103206456703088 1103 98-99 -1.2293430461388795 1103 100 -1.246388317389119 1104 1 -0.37036700131632827 1104 2 -0.33282410119487693 1104 3 -0.38035213450676864 1104 4 -0.254358036091233 1104 5 -0.283177976052464 1104 6 -0.3759791289590311 1104 7 -0.3158605832388304 1104 8 -0.3304087608341604 1104 9 -0.5869829713910661 1104 10-11 -0.555954695633055 1104 12-13 -0.5481573169134251 1104 14-15 -0.7275255140797086 1104 16-17 -0.6650136987231576 1104 18-19 -0.7573587468070428 1104 20-21 -0.7695343715998675 1104 22-23 -0.7292104703265849 1104 24-25 -0.8375552554871106 1104 26-27 -0.8552567279282144 1104 28-29 -0.8735290587899911 1104 30-31 -0.8881285294057406 1104 32-33 -0.8329934245281692 1104 34-35 -0.785863352982247 1104 36-37 -0.8480221213216552 1104 38-39 -0.8728453781508563 1104 40-41 -0.8751451071486613 1104 42-43 -0.722233195394189 1104 44-45 -0.9124344635600963 1104 46-47 -0.8972200680620332 1104 48-49 -0.8796899578687558 1104 50-51 -0.8506930150689556 1104 52-53 -0.857471472861512 1104 54-55 -0.9676713472651457 1104 56-57 -0.9550181403137756 1104 58-59 -1.0167200689999518 1104 60-61 -0.9139984294991734 1104 62-63 -0.9959860273517265 1104 64-65 -0.9032047956309981 1104 66-67 -0.9298044196990531 1104 68-69 -0.9554530971089648 1104 70-71 -0.9931828984168547 1104 72-73 -1.00628998063533 1104 74-75 -0.9889126790749714 1104 76-77 -0.9431421870841881 1104 78-79 -0.7346465408676508 1104 80-81 -0.7366724939854521 1104 82-83 -0.8480434893278783 1104 84-85 -0.8452130046318658 1104 86-87 -0.8705191560125094 1104 88-89 -0.898561559679866 1104 90-91 -0.9294492220674897 1104 92-93 -0.9894552633853557 1104 94-95 -0.9953292486817027 1104 96-97 -1.0105679260444091 1104 98-99 -1.121970930393406 1104 100 -1.2701785070431733 1105 1 -0.4016609223873324 1105 2 -0.39750782747964664 1105 3 -0.4236668742287222 1105 4 -0.27487698092314616 1105 5 -0.28131376148612475 1105 6 -0.3921832984900888 1105 7 -0.3774911488932915 1105 8 -0.48494173205192936 1105 9 -0.5586645450281651 1105 10-11 -0.5115912086244023 1105 12-13 -0.5939507168939002 1105 14-15 -0.7929846959142139 1105 16-17 -0.7623739754794769 1105 18-19 -0.8026104112078443 1105 20-21 -0.8476154528635718 1105 22-23 -0.8591883840176919 1105 24-25 -0.8751634342964465 1105 26-27 -0.838575497379253 1105 28-29 -0.838906975306827 1105 30-31 -0.7864265233886059 1105 32-33 -0.7837212370651443 1105 34-35 -0.8344767498026329 1105 36-37 -0.7399867316272477 1105 38-39 -0.8561605946930939 1105 40-41 -0.915537313746924 1105 42-43 -0.8688714787468612 1105 44-45 -0.8656466309711064 1105 46-47 -0.8469543362176921 1105 48-49 -0.9579245364141613 1105 50-51 -0.906121322624081 1105 52-53 -0.8688155038925487 1105 54-55 -0.9766568113357152 1105 56-57 -0.9562839719882064 1105 58-59 -1.063237183220501 1105 60-61 -0.9672017528236552 1105 62-63 -1.0675695440265045 1105 64-65 -1.0087198946051572 1105 66-67 -0.9216239717114547 1105 68-69 -0.9819404389499482 1105 70-71 -0.9573812741166066 1105 72-73 -0.9424757125620218 1105 74-75 -0.9308846384229881 1105 76-77 -0.9066900899324501 1105 78-79 -0.976886726567237 1105 80-81 -0.9065342245704713 1105 82-83 -0.7600282675376278 1105 84-85 -0.7414436207542323 1105 86-87 -0.808664375249144 1105 88-89 -0.8570849189843521 1105 90-91 -0.854349496151162 1105 92-93 -0.8591003512058712 1105 94-95 -0.8591275934147014 1105 96-97 -0.9890225143164848 1105 98-99 -1.0313620104705379 1105 100 -1.1538336107792269 1106 1 -0.4089767214142519 1106 2 -0.38605045079146194 1106 3 -0.4601042674349429 1106 4 -0.3339209350733725 1106 5 -0.3377791537026411 1106 6 -0.3858308549632099 1106 7 -0.43474154966970957 1106 8 -0.4548585860099408 1106 9 -0.5786758521946567 1106 10-11 -0.5756692129882239 1106 12-13 -0.6525473115937999 1106 14-15 -0.8082933302377242 1106 16-17 -0.7525750515295186 1106 18-19 -0.7472730547794271 1106 20-21 -0.7282436253181999 1106 22-23 -0.6630617362105156 1106 24-25 -0.7883756353455951 1106 26-27 -0.846007229295779 1106 28-29 -0.8061847634965105 1106 30-31 -0.8762791367033529 1106 32-33 -0.8816620898937444 1106 34-35 -0.9048665584645761 1106 36-37 -0.9276054499067996 1106 38-39 -0.8950758002905346 1106 40-41 -0.9627948175751442 1106 42-43 -0.8472504416455493 1106 44-45 -0.8428868182411335 1106 46-47 -0.8628355598118205 1106 48-49 -1.0336566287138282 1106 50-51 -0.9959206548519504 1106 52-53 -0.975441026312005 1106 54-55 -0.9602532543661084 1106 56-57 -0.9909795926091647 1106 58-59 -1.0035489206200907 1106 60-61 -1.0637027865538045 1106 62-63 -1.0233332475301609 1106 64-65 -0.9312451606949566 1106 66-67 -0.9347234618231823 1106 68-69 -0.9745362043046057 1106 70-71 -1.0124955042085872 1106 72-73 -1.0900673812003916 1106 74-75 -1.1164541869250115 1106 76-77 -1.0958292826932414 1106 78-79 -0.891729997860363 1106 80-81 -0.7781228289929558 1106 82-83 -0.8636202441060838 1106 84-85 -0.854593834067142 1106 86-87 -0.9560434032427452 1106 88-89 -0.9657254650470364 1106 90-91 -0.9573631036111365 1106 92-93 -1.0448045658308445 1106 94-95 -1.0472612747516585 1106 96-97 -1.0499727573730162 1106 98-99 -1.0245639127504518 1106 100 -1.0579786153514483 1107 1 -0.4169217659300024 1107 2 -0.423870677127983 1107 3 -0.4288215543426759 1107 4 -0.2464623416672893 1107 5 -0.28122515314358054 1107 6 -0.3471383910888406 1107 7 -0.4443356604343336 1107 8 -0.4918516010795102 1107 9 -0.608645438538808 1107 10-11 -0.5633359482822868 1107 12-13 -0.5877940103646964 1107 14-15 -0.8263805004286553 1107 16-17 -0.784144158486157 1107 18-19 -0.7686969394586143 1107 20-21 -0.7008115789997262 1107 22-23 -0.7690988552656677 1107 24-25 -0.7990364837476704 1107 26-27 -0.8001319872168082 1107 28-29 -0.8509891670323313 1107 30-31 -0.8546415027753014 1107 32-33 -0.7928989900107482 1107 34-35 -0.8046579981168236 1107 36-37 -0.8886915371238544 1107 38-39 -0.8399656533107276 1107 40-41 -0.8771149292536862 1107 42-43 -0.7847412564536782 1107 44-45 -0.9104658108913029 1107 46-47 -0.8573844518140561 1107 48-49 -0.8692272153442033 1107 50-51 -0.9881419899394643 1107 52-53 -0.9682830209255897 1107 54-55 -0.9309688540440604 1107 56-57 -1.0023137427759607 1107 58-59 -1.0205497692564975 1107 60-61 -0.9987911401489526 1107 62-63 -0.9308446840355913 1107 64-65 -0.8822061953953124 1107 66-67 -0.9206778597516347 1107 68-69 -0.9440637967047039 1107 70-71 -0.9818272574378355 1107 72-73 -1.0288526594510259 1107 74-75 -0.9347572823555979 1107 76-77 -0.9776004220252794 1107 78-79 -0.8107376121542771 1107 80-81 -0.8324423380894359 1107 82-83 -0.7922585425482396 1107 84-85 -0.7565153176241814 1107 86-87 -0.8277323403382013 1107 88-89 -0.8176458606086854 1107 90-91 -0.8465294313253935 1107 92-93 -0.8306658146152195 1107 94-95 -0.8067739532456741 1107 96-97 -0.8801000193572825 1107 98-99 -0.9932580169871059 1107 100 -1.1172405527532163 1108 1 -0.4669838103284718 1108 2 -0.42026976646650027 1108 3 -0.4901771685991463 1108 4 -0.29307474458963867 1108 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34-35 0.9812430306356887 2311 36-37 1.0121607719277321 2311 38-39 1.018103632436116 2311 40-41 0.9582413093601545 2311 42-43 0.8919307070723903 2311 44-45 0.9369241432877047 2311 46-47 0.7068579308279084 2311 48-49 0.9543394776382144 2311 50-51 1.0062472825419064 2311 52-53 0.891951420167544 2311 54-55 0.9145691225815114 2311 56-57 1.051562777000406 2311 58-59 1.0440103072000326 2311 60-61 1.1141389156793196 2311 62-63 1.0847540566315885 2311 64-65 1.1908107135042059 2311 66-67 1.1642204043921396 2311 68-69 1.2446172728388447 2311 70-71 1.2308271271274478 2311 72-73 1.2084888278411583 2311 74-75 1.2074689819677005 2311 76-77 1.1866445026104948 2311 78-79 0.9352659995548507 2311 80-81 0.9040261301743584 2311 82-83 1.003096098834611 2311 84-85 1.0705253181208505 2311 86-87 1.136788553396343 2311 88-89 1.15713016341655 2311 90-91 1.1970865001463267 2311 92-93 1.2425080260382053 2311 94-95 1.2762432770394376 2311 96-97 1.2682159201352299 2311 98-99 1.3063003410261942 2311 100 1.358272815709963 2312 1 0.26937176831401644 2312 2 0.3688244508162768 2312 3 0.48197077484709183 2312 4 0.19058367926047737 2312 5 0.3348725231641154 2312 6 0.39904440717601375 2312 7 0.3234272023834137 2312 8 0.4609789319680502 2312 9 0.6520911939414447 2312 10-11 0.6676049114262312 2312 12-13 0.7236747460437911 2312 14-15 0.8966792164293267 2312 16-17 0.8699525033285624 2312 18-19 0.8682024641279042 2312 20-21 0.8650029030250082 2312 22-23 0.9681030806884152 2312 24-25 0.9496123604304145 2312 26-27 0.9955229748655583 2312 28-29 0.9296466777166827 2312 30-31 1.0015238045633836 2312 32-33 0.8314272132176654 2312 34-35 0.986026691112869 2312 36-37 1.0001852681365406 2312 38-39 0.8275020011295311 2312 40-41 0.9389705335268985 2312 42-43 1.0557164509765116 2312 44-45 0.7992408897825172 2312 46-47 0.832536341812137 2312 48-49 1.020650053837663 2312 50-51 0.8379959621243671 2312 52-53 1.0093895580693157 2312 54-55 0.9297619536290611 2312 56-57 0.9639439472416953 2312 58-59 1.0465530266921874 2312 60-61 1.1514978170482664 2312 62-63 1.1184560433982327 2312 64-65 1.1361274641363934 2312 66-67 1.1470186745362625 2312 68-69 1.218184913069571 2312 70-71 1.1205610441488076 2312 72-73 1.1109135257761906 2312 74-75 1.132698818748203 2312 76-77 1.1311835384227003 2312 78-79 0.9970472804003805 2312 80-81 0.9630501139370509 2312 82-83 0.917234703357515 2312 84-85 0.9928536432646098 2312 86-87 1.006362786533252 2312 88-89 1.0005856894350735 2312 90-91 1.102126138544989 2312 92-93 1.1618284159373893 2312 94-95 1.2016614196595832 2312 96-97 0.8488119231753188 2312 98-99 0.6336775554195135 2312 100 0.9184147215443126 2313 1 -0.010437711668437544 2313 2 0.18584891759397237 2313 3 0.2625294265800733 2313 4 0.16607226676521947 2313 5 0.32348498794384994 2313 6 0.2513692856541496 2313 7 0.25675540155099696 2313 8 0.3937363320767915 2313 9 0.5900804908494592 2313 10-11 0.3690780241801548 2313 12-13 0.41016522409818634 2313 14-15 0.45581656099561485 2313 16-17 0.7010959294857031 2313 18-19 0.6815859182772357 2313 20-21 0.6753983393138441 2313 22-23 0.6411296244506417 2313 24-25 0.5580260782094228 2313 26-27 0.8187186995585876 2313 28-29 0.6052357423991239 2313 30-31 0.8596477801853268 2313 32-33 0.9474312693876428 2313 34-35 0.9400829959094068 2313 36-37 0.9826537339190153 2313 38-39 0.9725109909864287 2313 40-41 0.987408578184926 2313 42-43 1.0970875069857087 2313 44-45 0.8992043053602927 2313 46-47 0.9001277430592083 2313 48-49 0.9622050428207132 2313 50-51 0.9245389609970189 2313 52-53 0.9010060963465705 2313 54-55 0.9131449386564441 2313 56-57 0.6455586293343387 2313 58-59 0.7268105047104072 2313 60-61 0.5639167770905189 2313 62-63 0.9288813973567613 2313 64-65 1.0319745113015983 2313 66-67 1.009213408358221 2313 68-69 1.0579987699480213 2313 70-71 1.002449462713777 2313 72-73 1.0214552997539172 2313 74-75 1.077430768835299 2313 76-77 1.0726732993661194 2313 78-79 0.9500544632944852 2313 80-81 0.8349170316342693 2313 82-83 0.9064769055424975 2313 84-85 0.8944715886928662 2313 86-87 0.9331932116023225 2313 88-89 0.9338919095893097 2313 90-91 0.808076540677682 2313 92-93 0.94190351863422 2313 94-95 1.1874078253953222 2313 96-97 1.2314034768037736 2313 98-99 1.2110227561596112 2313 100 1.1550886797790945 2314 1 -0.4241796595571472 2314 2 -0.011947700451010235 2314 3 0.22951600820824325 2314 4 0.21652645081780264 2314 5 0.3206113514848994 2314 6 0.37572343481007664 2314 7 0.3963020702610933 2314 8 0.4293917941893426 2314 9 0.44029846667304184 2314 10-11 0.5520887675831645 2314 12-13 0.45840971943438547 2314 14-15 0.7744484615359326 2314 16-17 0.8019702915583906 2314 18-19 0.8316892128292466 2314 20-21 0.864918633824395 2314 22-23 0.9211852369076112 2314 24-25 0.8121655614277898 2314 26-27 0.7117261738311029 2314 28-29 0.7285256242809481 2314 30-31 0.7966303350854957 2314 32-33 0.8872641297638708 2314 34-35 0.9074436387228246 2314 36-37 0.884492075726321 2314 38-39 0.6693112193497441 2314 40-41 0.6497765375449234 2314 42-43 0.8082504973666644 2314 44-45 0.8976971838609344 2314 46-47 0.8303606927086449 2314 48-49 0.8304509844606329 2314 50-51 0.8450880321725407 2314 52-53 0.9255049162156936 2314 54-55 0.8501499452960743 2314 56-57 0.8333988344756875 2314 58-59 0.8617290894586702 2314 60-61 0.9054575150658053 2314 62-63 0.8513666970273377 2314 64-65 0.8624474157914364 2314 66-67 0.6090144262614388 2314 68-69 0.6265590690413916 2314 70-71 0.7707606793855959 2314 72-73 0.8892453898819568 2314 74-75 0.927132266726808 2314 76-77 0.860742512250031 2314 78-79 0.7355002206780838 2314 80-81 0.7820928779608991 2314 82-83 0.7989429675738009 2314 84-85 0.8259136739007857 2314 86-87 0.8822363537394438 2314 88-89 0.9129660815716427 2314 90-91 1.0136350472729987 2314 92-93 0.8679787123180951 2314 94-95 0.9584182262864687 2314 96-97 0.9172945751199073 2314 98-99 1.0198873845731349 2314 100 1.0398206425243295 2315 1 0.36352898857404625 2315 2 0.4001110547060094 2315 3 0.4070700826992706 2315 4 0.3203778298773088 2315 5 0.34804194467834293 2315 6 0.43237717327002656 2315 7 0.4117947776163007 2315 8 0.29553237023515067 2315 9 0.5543367262854488 2315 10-11 0.5990610841993913 2315 12-13 0.44350156477128877 2315 14-15 0.6565383514802079 2315 16-17 0.7988259588340867 2315 18-19 0.7423825560706874 2315 20-21 0.5364697688597815 2315 22-23 0.8709700261796982 2315 24-25 0.9013385583338973 2315 26-27 0.826891461481793 2315 28-29 0.833446527033054 2315 30-31 0.907996744450486 2315 32-33 0.8275183797798675 2315 34-35 0.8592527931861014 2315 36-37 0.8762698113496654 2315 38-39 0.8848388129467324 2315 40-41 0.9227541150129497 2315 42-43 1.0296275358528604 2315 44-45 1.0324488434469643 2315 46-47 1.0002527314625667 2315 48-49 0.8903382302334677 2315 50-51 0.9853604354411729 2315 52-53 1.0321616013142219 2315 54-55 0.8708950155125947 2315 56-57 0.881539399420582 2315 58-59 0.8906998522740182 2315 60-61 0.9799037673986248 2315 62-63 1.0997365365734737 2315 64-65 1.1653880223320954 2315 66-67 1.1966419887758803 2315 68-69 1.1764753714458465 2315 70-71 1.0441001383533646 2315 72-73 1.1523050389887217 2315 74-75 0.5316958181298972 2315 76-77 0.5682691658611567 2315 78-79 0.8385013598273332 2315 80-81 0.9223449567695141 2315 82-83 0.8820164634331675 2315 84-85 0.9128363694498418 2315 86-87 0.971163375704883 2315 88-89 0.9560311635943002 2315 90-91 0.9946331872176017 2315 92-93 1.0534999751314835 2315 94-95 1.015227377484777 2315 96-97 1.1774255021895392 2315 98-99 1.1982829651703106 2315 100 1.091846525928208 2316 1 0.26531033839423657 2316 2 0.055094168266400345 2316 3 0.17794227159532028 2316 4 0.02866625051104421 2316 5 0.2752724725665061 2316 6 0.36283667604956804 2316 7 0.4224169221818741 2316 8 0.4372216005027738 2316 9 0.6296790413415465 2316 10-11 0.6205276641254045 2316 12-13 0.41382390761871335 2316 14-15 0.5260367143168025 2316 16-17 0.8077626054723623 2316 18-19 0.7972168356656724 2316 20-21 0.7652920929127234 2316 22-23 0.8422100967652568 2316 24-25 0.5046053315980075 2316 26-27 0.7530632110362419 2316 28-29 0.9384459223408115 2316 30-31 0.5838306119967385 2316 32-33 0.6506485630751655 2316 34-35 0.7790939102978314 2316 36-37 0.8597989342272214 2316 38-39 0.787818577002362 2316 40-41 0.8121530018889231 2316 42-43 0.7978976890606404 2316 44-45 0.6231791666386286 2316 46-47 0.8040491313782425 2316 48-49 0.9238898298896103 2316 50-51 0.9538280839973083 2316 52-53 0.9372827879737358 2316 54-55 0.8546826851839313 2316 56-57 0.9785793389350133 2316 58-59 0.7837992616367799 2316 60-61 0.9298445596516984 2316 62-63 1.0137730728554217 2316 64-65 1.0732521522739233 2316 66-67 0.9783682809582785 2316 68-69 0.953783630888239 2316 70-71 0.9492989442087492 2316 72-73 0.8880849142586911 2316 74-75 0.9213081450265364 2316 76-77 0.8399119134765023 2316 78-79 0.5297357277155612 2316 80-81 0.5280827303416871 2316 82-83 0.5446453078254194 2316 84-85 0.6725537074883334 2316 86-87 0.7067360315214088 2316 88-89 0.6483646362520012 2316 90-91 0.5748216765329346 2316 92-93 0.45399377989597056 2316 94-95 0.5066476627034042 2316 96-97 0.7067141032095101 2316 98-99 0.8762590571505378 2316 100 0.49534785747688304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 34.0 13 182.0 14 711.0 15 1934.0 16 4631.0 17 9663.0 18 16121.0 19 23970.0 20 35008.0 21 49884.0 22 61781.0 23 65518.0 24 66301.0 25 72208.0 26 84922.0 27 103431.0 28 127353.0 29 158082.0 30 194664.0 31 244461.0 32 309270.0 33 410539.0 34 594035.0 35 830505.0 36 1297798.0 37 1882876.0 38 1424194.0 39 209925.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52969001032691 20.36382322489738 13.617466066617798 23.489020698157915 2 15.71273033595913 19.74281478618724 40.582160078019086 23.96229479983454 3 19.112152439153263 25.504524442860983 29.130757455442463 26.252565662543287 4 12.839658064973648 16.066872534499936 37.88036188312891 33.2131075173975 5 13.437145419246924 37.858025790984925 34.16615287997319 14.538675909794959 6 31.48823826015018 36.73231662947878 17.33783412489697 14.441610985474076 7 28.150820644655756 31.882121567342043 21.92613630462198 18.04092148338022 8 26.951186741585925 35.48578657673135 19.090968928233913 18.472057753448812 9 26.683228034134597 15.537690236398685 20.703476836330296 37.07560489313642 10-11 26.361855838438412 25.914946978184027 26.702254882494998 21.02094230088256 12-13 26.14037712934165 23.332655799065314 27.605831818844965 22.92113525274806 14-15 23.394580009164237 24.670411185295098 25.265440372443816 26.669568433096853 16-17 22.461745169082125 27.495615942028984 26.32725845410628 23.71538043478261 18-19 22.090337891491874 27.098203205908195 28.633530695861424 22.177928206738507 20-21 23.259230694305312 26.346715519984272 28.267048002092267 22.127005783618152 22-23 23.44239902657299 25.968804442015575 28.098562504340265 22.490234027071175 24-25 22.902042266100274 25.935235989410106 28.662782433122636 22.499939311366987 26-27 22.858588168527795 26.226538955338697 27.929490854169796 22.985382021963712 28-29 22.3110311648797 26.686980250445362 28.34506409684675 22.656924487828185 30-31 23.184846852180563 26.261650175348205 28.24901437967606 22.304488592795174 32-33 23.335222497383075 26.406346141720643 27.670944584440683 22.587486776455602 34-35 23.198103487453565 26.393642324392413 28.200434383905833 22.20781980424819 36-37 22.972462799893204 26.143904039006806 27.974401784107094 22.909231376992896 38-39 23.121442096584715 26.244617656562852 28.29880801741919 22.335132229433242 40-41 22.809744288779108 25.571405908991846 28.783259810965255 22.83558999126379 42-43 22.820794422138064 25.86283472675816 28.08712323529229 23.229247615811484 44-45 22.77987782300503 26.461898517517913 28.0743592157776 22.68386444369946 46-47 22.78921593911537 26.292885756643404 27.541128639966022 23.376769664275205 48-49 23.384056077535188 26.257554499981612 27.52297910426166 22.835410318221548 50-51 23.098341216127462 26.24746689181171 27.482443004877034 23.171748887183792 52-53 22.708827937909664 26.66947936138946 27.195192780208018 23.42649992049286 54-55 22.614032819295186 26.51052165826236 27.325607975036668 23.549837547405787 56-57 23.348396752774836 26.16130992872276 26.984480964203772 23.50581235429863 58-59 22.56695555589375 26.606742504014232 27.5256851486684 23.30061679142362 60-61 22.838238259407724 26.98628566038399 27.05200728753384 23.123468792674444 62-63 23.09071966218995 26.514593529562696 26.79614216056086 23.598544647686488 64-65 22.46717534638102 26.518667856060045 27.043540943368882 23.970615854190054 66-67 22.768297502769943 26.27651385859932 27.07218263807859 23.883006000552147 68-69 22.41111398655513 26.366473533907737 27.26908433972699 23.953328139810143 70-71 22.40755034119666 26.0079854730488 27.414696303403268 24.16976788235127 72-73 22.741050400206085 26.2658894695608 27.325948655886567 23.66711147434655 74-75 22.946465134550433 26.512316953437328 26.99583600463282 23.54538190737942 76-77 22.753533210420148 26.28823564731237 26.76842945726713 24.18980168500035 78-79 22.554903202801583 26.343445243920822 26.95576579658383 24.145885756693765 80-81 22.242576670215612 26.74223617806269 27.178381353989074 23.836805797732623 82-83 22.960516105772612 25.97099819792129 27.88891834863599 23.1795673476701 84-85 23.09316532266591 26.362032537566467 26.93111671388342 23.613685425884203 86-87 22.12695117912568 26.8869331034744 27.247219575599225 23.73889614180069 88-89 22.56962239417391 25.963276191415424 27.276426603656525 24.190674810754146 90-91 22.55051324173525 25.883710236626545 27.341115821600926 24.224660700037283 92-93 22.747033284447124 26.10509123590086 27.14823600966254 23.99963946998947 94-95 21.93288835586183 26.029793012881754 27.62731399417871 24.410004637077705 96-97 22.05810676984746 25.983143243060606 27.35801081837865 24.600739168713286 98-99 21.611686743815167 26.360936402619206 27.22632025139661 24.801056602169012 100 21.9560549326631 26.054113558785957 26.799595135341885 25.190236373209057 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1930.0 1 1372.0 2 1447.5 3 1992.5 4 1457.5 5 1130.5 6 1740.5 7 3299.5 8 5192.0 9 5995.0 10 5121.5 11 4260.5 12 4502.0 13 5159.5 14 6008.5 15 7009.5 16 8078.5 17 9075.0 18 9947.5 19 10879.5 20 11790.0 21 12617.0 22 13839.0 23 15736.0 24 18385.0 25 21398.5 26 25143.0 27 29782.5 28 36331.5 29 47790.0 30 63876.0 31 80015.5 32 101208.5 33 124581.5 34 142490.0 35 161059.0 36 180813.5 37 210793.0 38 227045.0 39 222410.0 40 216063.0 41 209366.0 42 213210.0 43 227351.5 44 247956.0 45 286028.5 46 322411.0 47 347608.5 48 369544.0 49 375243.0 50 370392.5 51 377957.5 52 380638.0 53 364491.5 54 348669.0 55 315189.5 56 278414.0 57 238239.5 58 198700.0 59 181112.0 60 149768.5 61 109515.0 62 83800.0 63 67785.0 64 49858.0 65 30507.0 66 20360.5 67 14751.5 68 11004.5 69 8291.5 70 6355.0 71 4856.5 72 3720.0 73 2894.0 74 2219.5 75 1562.5 76 995.0 77 549.5 78 327.5 79 209.5 80 129.5 81 83.5 82 51.0 83 32.5 84 23.5 85 15.0 86 8.5 87 6.0 88 5.0 89 5.5 90 4.5 91 2.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04333320249636927 2 0.0 3 0.0 4 0.030748699430255343 5 0.0 6 0.0 7 8.57485333679548E-4 8 0.0 9 0.011086923046729931 10-11 4.468585541710321E-4 12-13 1.2077258220838705E-5 14-15 1.3284984042922574E-4 16-17 3.0193145552096765E-4 18-19 0.0 20-21 6.0386291104193525E-6 22-23 0.0 24-25 6.763264603669675E-4 26-27 5.97824281931516E-4 28-29 1.8719750242299994E-4 30-31 0.0025845332592594828 32-33 0.014867104869852444 34-35 0.025289778714436245 36-37 0.03013275926099257 38-39 0.021739064797509672 40-41 0.0014311550991693866 42-43 7.850217843545158E-5 44-45 0.011720979103323963 46-47 0.018695595725858317 48-49 0.0033152073816202247 50-51 0.011080884417619513 52-53 0.010803107478540222 54-55 0.010150935534614932 56-57 3.925108921772579E-4 58-59 0.008780166726549738 60-61 0.004909405466770934 62-63 0.0012741507422984835 64-65 0.0359479590943264 66-67 0.06082711102925414 68-69 0.060078321019562134 70-71 0.05280781157061724 72-73 0.04576073139875785 74-75 0.03621365877518486 76-77 0.031859807186572504 78-79 0.015374349715127671 80-81 0.0011896099347526124 82-83 0.03858080138646924 84-85 0.05265684584285675 86-87 0.05802518712201955 88-89 0.07006621356819574 90-91 0.04893101168172801 92-93 0.04011461318051576 94-95 0.01333329307580593 96-97 0.0038466067433371278 98-99 0.02163036947352212 100 0.03249990187227696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8280025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.60214112681717 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20604927570893 42.90031230991827 2 15.329428547448837 17.966746702621137 3 5.257773394552498 9.243503354411704 4 2.3543742389914577 5.518854856748807 5 1.2072267631147051 3.537303657205931 6 0.6979342936805794 2.4540266385308867 7 0.44759306499342394 1.8360938373490499 8 0.30556624887146966 1.4325469151966406 9 0.2130982515139349 1.1239212428187837 >10 0.9280066659794752 9.096770958753357 >50 0.03681961854448943 1.4853960955736287 >100 0.014775398185599272 1.5408084841218308 >500 7.85479087527324E-4 0.30081538879446845 >1k 4.33340440153822E-4 0.45710419657984763 >5k 5.4167554806500856E-5 0.18036569715768683 >10k+ 8.125133220975128E-5 0.9254296642179907 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24811 0.29964885371722916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20560 0.24830842902044378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12936 0.1562314123447695 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.0386291104193526E-5 0.0 0.0 0.0 0.0 7 6.0386291104193526E-5 0.0 0.0 0.0 0.0 8 6.0386291104193526E-5 0.0 0.0 0.0 1.2077258220838705E-5 9 6.0386291104193526E-5 0.0 0.0 0.0 1.2077258220838705E-5 10-11 6.0386291104193526E-5 0.0 0.0 0.0 4.227040377293547E-5 12-13 6.0386291104193526E-5 0.0 0.0 0.0 8.454080754587094E-5 14-15 1.0869532398754835E-4 6.0386291104193525E-6 0.0 6.0386291104193525E-6 9.057943665629028E-5 16-17 1.9323613153341928E-4 1.2077258220838705E-5 0.0 7.246354932503223E-5 1.0869532398754835E-4 18-19 1.9323613153341928E-4 1.2077258220838705E-5 0.0 9.661806576670965E-5 1.2681121131880642E-4 20-21 1.9323613153341928E-4 1.2077258220838705E-5 0.0 1.388884695396451E-4 1.3284984042922577E-4 22-23 2.05313389754258E-4 1.2077258220838705E-5 0.0 3.200473428522257E-4 1.630429859813225E-4 24-25 2.173906479750967E-4 1.2077258220838705E-5 0.0 7.548286388024191E-4 2.05313389754258E-4 26-27 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.001455309615611064 2.05313389754258E-4 28-29 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.004184769973520611 2.173906479750967E-4 30-31 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.014728216400312803 2.173906479750967E-4 32-33 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.03952282752769466 2.777769390792902E-4 34-35 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.07544059347646898 2.898541973001289E-4 36-37 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.1352350989278414 2.898541973001289E-4 38-39 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.2275053517350491 2.898541973001289E-4 40-41 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.32736616133405394 2.898541973001289E-4 42-43 2.173906479750967E-4 1.2077258220838705E-5 0.0 0.42401442024631564 2.9589282641054825E-4 44-45 2.2342927708551605E-4 1.2077258220838705E-5 0.0 0.5349923460376025 3.07970084631387E-4 46-47 2.294679061959354E-4 1.2077258220838705E-5 0.0 0.6643035498081226 3.26085971962645E-4 48-49 2.294679061959354E-4 1.2077258220838705E-5 0.0 0.8095869275757017 3.5024048840432245E-4 50-51 2.415451644167741E-4 1.2077258220838705E-5 0.0 0.9595442042747455 3.5024048840432245E-4 52-53 2.536224226376128E-4 1.2077258220838705E-5 0.0 1.105999076089746 3.683563757355805E-4 54-55 2.536224226376128E-4 1.2077258220838705E-5 0.0 1.2644164721724875 3.7439500484599987E-4 56-57 2.536224226376128E-4 1.2077258220838705E-5 0.0 1.449120020772884 3.7439500484599987E-4 58-59 2.596610517480322E-4 1.2077258220838705E-5 0.0 1.6612932931965785 3.7439500484599987E-4 60-61 2.717383099688709E-4 1.2077258220838705E-5 0.0 1.8722528011690787 3.7439500484599987E-4 62-63 3.1400871374180633E-4 1.2077258220838705E-5 0.0 2.0890697793786854 3.9854952128767724E-4 64-65 3.1400871374180633E-4 1.2077258220838705E-5 0.0 2.3119797343607056 4.2270403772935467E-4 66-67 3.1400871374180633E-4 1.2077258220838705E-5 0.0 2.5554572600928136 4.2270403772935467E-4 68-69 3.1400871374180633E-4 1.2077258220838705E-5 0.0 2.828409334512879 4.2270403772935467E-4 70-71 3.1400871374180633E-4 1.2077258220838705E-5 0.0 3.12002681151325 4.589358123918708E-4 72-73 4.10626779508516E-4 1.2077258220838705E-5 0.0 3.4072058961174636 5.072448452752256E-4 74-75 4.10626779508516E-4 1.2077258220838705E-5 0.0 3.6898741247761935 5.072448452752256E-4 76-77 4.1666540861893533E-4 1.2077258220838705E-5 0.0 4.009879197224646 5.072448452752256E-4 78-79 4.2270403772935467E-4 1.2077258220838705E-5 0.0 4.359835870060779 5.072448452752256E-4 80-81 4.2270403772935467E-4 1.2077258220838705E-5 0.0 4.722672938789436 5.132834743856449E-4 82-83 4.2270403772935467E-4 1.2077258220838705E-5 0.0 5.09122858928566 5.434766199377418E-4 84-85 4.2270403772935467E-4 1.2077258220838705E-5 0.0 5.452284262426744 5.555538781585804E-4 86-87 4.28742666839774E-4 1.2077258220838705E-5 0.0 5.833732386073713 5.917856528210965E-4 88 4.468585541710321E-4 1.2077258220838705E-5 0.0 6.139208516882498 6.038629110419353E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15240 0.0 46.64234 1 GTATCAA 25815 0.0 37.806698 1 GTACTAG 6415 0.0 32.68524 1 TACCTGG 5590 0.0 32.114983 2 CAACGCA 29615 0.0 31.478205 5 ATCAACG 29655 0.0 31.396936 3 TATCAAC 32775 0.0 29.566647 2 CTAACGC 2915 0.0 28.700016 3 AACGCAG 33345 0.0 28.520674 6 GTGGTAT 5205 0.0 27.819128 1 TCAACGC 33990 0.0 27.683031 4 TAGGCAT 8270 0.0 27.669638 5 CTGTGCG 9025 0.0 27.645683 9 GGGCACC 8020 0.0 27.373302 94 TCTAACG 3040 0.0 27.362501 2 GTACATG 34300 0.0 27.193247 1 TACATGG 34405 0.0 26.786263 2 TAGTACT 9255 0.0 26.559872 4 ACCTGGG 6640 0.0 26.473064 3 TAACGCC 3210 0.0 26.062477 4 >>END_MODULE