FastQCFastQC Report
Mon 6 Jun 2016
ERR1211202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8475640
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354970.41881203071390477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283350.3343110372786008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166810.19681109627119603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG49100.038.9148332
GTACTAG83600.036.535211
ACCTGGG52850.035.489793
GCATCGT79100.035.299638
GGTATCA265550.033.229831
TAGGCAT100000.032.0129135
GGGCACC85600.031.95472394
GTATCAA407600.031.9252381
CTAGGCA109600.029.9809044
GTACCTG67350.028.859291
CTGTGCG107750.028.0073999
CAACGCA447900.027.802195
GTACATG418650.027.7776091
TAACGCC38650.027.4876164
CTAACGC39200.027.4615443
ATCAACG455050.027.3341943
CATCGTC106650.027.0180919
TACATGG425050.027.015962
CCTGGGG52550.026.8365484
TAGTACT116600.026.7296374
Produced by FastQC (version 0.11.5)