##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8475640 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496746440386804 31.0 31.0 34.0 30.0 34.0 2 31.510104015743945 31.0 31.0 34.0 28.0 34.0 3 31.569977724396033 33.0 31.0 34.0 28.0 34.0 4 35.170221953740366 37.0 35.0 37.0 32.0 37.0 5 35.24014906248968 37.0 35.0 37.0 32.0 37.0 6 35.30342345828752 37.0 35.0 37.0 32.0 37.0 7 35.22365154725779 37.0 35.0 37.0 32.0 37.0 8 35.23410538909156 37.0 35.0 37.0 32.0 37.0 9 36.798738030402426 39.0 37.0 39.0 32.0 39.0 10-11 36.754458542363764 39.0 37.0 39.0 32.0 39.0 12-13 36.64210449004441 39.0 37.0 39.0 32.0 39.0 14-15 37.819036910487 40.0 37.0 41.0 32.0 41.0 16-17 37.80630737029888 40.0 37.0 41.0 32.0 41.0 18-19 37.8453730337768 40.0 37.5 41.0 32.0 41.0 20-21 37.82087500176978 40.0 37.5 41.0 32.0 41.0 22-23 37.704719053664384 40.0 37.0 41.0 32.0 41.0 24-25 37.606651946047734 40.0 37.0 41.0 32.0 41.0 26-27 37.451742936226644 40.0 37.0 41.0 31.5 41.0 28-29 37.34423459467368 40.0 36.0 41.0 31.0 41.0 30-31 37.189386170247914 40.0 36.0 41.0 31.0 41.0 32-33 36.991486955557335 39.0 36.0 41.0 30.5 41.0 34-35 36.83944256716897 39.0 35.0 41.0 30.0 41.0 36-37 36.72480915895437 39.0 35.0 41.0 30.0 41.0 38-39 36.575199159001556 39.0 35.0 41.0 30.0 41.0 40-41 36.40939250605264 39.0 35.0 41.0 29.5 41.0 42-43 36.19200066307677 39.0 35.0 40.0 28.5 41.0 44-45 35.93522908004587 38.5 35.0 40.0 27.5 41.0 46-47 35.66137630904569 38.0 34.5 40.0 27.0 41.0 48-49 35.409705107814865 38.0 34.0 40.0 26.0 41.0 50-51 34.70028092273858 37.5 33.5 39.5 25.0 40.5 52-53 34.78893074741258 38.0 33.5 39.5 25.5 40.5 54-55 35.24284408021106 38.0 34.0 40.0 26.0 41.0 56-57 35.17541931936704 38.0 34.0 40.0 26.0 41.0 58-59 34.9486129661005 37.5 34.0 40.0 26.0 41.0 60-61 34.72597243394009 37.0 34.0 40.0 26.0 41.0 62-63 34.42583498119316 36.5 33.0 40.0 25.5 41.0 64-65 34.18306033526672 36.0 33.0 39.5 25.0 41.0 66-67 34.017631175934795 35.5 33.0 39.0 25.0 41.0 68-69 33.73823404486269 35.0 33.0 39.0 24.5 41.0 70-71 33.46925087662997 35.0 33.0 38.5 24.0 41.0 72-73 33.162394993180456 35.0 32.5 37.5 24.0 40.0 74-75 32.84781981065737 35.0 32.0 37.0 23.0 39.5 76-77 32.54087980376703 35.0 32.0 36.5 23.0 39.0 78-79 32.21829348580167 35.0 32.0 36.0 22.0 39.0 80-81 31.937480414458378 35.0 31.0 36.0 21.5 38.5 82-83 31.648173766228865 35.0 31.0 35.5 20.0 37.0 84-85 31.404430815843995 35.0 31.0 35.0 20.0 37.0 86-87 31.186918392003435 34.5 31.0 35.0 20.0 36.5 88-89 30.98043274608171 34.0 31.0 35.0 19.0 36.0 90-91 30.74884073651075 34.0 30.0 35.0 19.0 36.0 92-93 30.54531940950772 34.0 30.0 35.0 18.0 35.5 94-95 30.380600698000386 34.0 30.0 35.0 18.0 35.0 96-97 30.1951184807283 34.0 29.0 35.0 17.5 35.0 98-99 29.967202358759927 34.0 29.0 35.0 16.5 35.0 100 28.817697778574832 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43087884986579184 1101 2 -0.35846665657867405 1101 3 -0.35699425492926196 1101 4 -0.34233019472343784 1101 5 -0.2496685808343173 1101 6 -0.24811636622172273 1101 7 -0.2856562031466652 1101 8 -0.26705188778989 1101 9 -0.38241891471760425 1101 10-11 -0.42648387544338817 1101 12-13 -0.46182139392919197 1101 14-15 -0.5860964078324074 1101 16-17 -0.6127659328039883 1101 18-19 -0.6135439421720861 1101 20-21 -0.6648540597827903 1101 22-23 -0.7373787275491992 1101 24-25 -0.8192240006720226 1101 26-27 -0.8117799327217483 1101 28-29 -0.7033777310101215 1101 30-31 -0.8258280953198494 1101 32-33 -0.7911693620531963 1101 34-35 -0.8214051716165116 1101 36-37 -0.830638037749992 1101 38-39 -0.8597799211498725 1101 40-41 -0.8019655706736373 1101 42-43 -0.8327601093377908 1101 44-45 -0.8386451310855421 1101 46-47 -0.9739359098492741 1101 48-49 -0.9417361395347967 1101 50-51 -0.9963568196127 1101 52-53 -0.8922159273468253 1101 54-55 -0.7918543809420484 1101 56-57 -0.6331965372675299 1101 58-59 -0.5950346862650662 1101 60-61 -0.48941119134833144 1101 62-63 -0.6641065627913179 1101 64-65 -0.6399851724535637 1101 66-67 -0.6734250481062034 1101 68-69 -0.7000014638226659 1101 70-71 -0.7777160816760897 1101 72-73 -0.7925986903968862 1101 74-75 -0.7955639823402834 1101 76-77 -0.826994282065943 1101 78-79 -0.7825205553445826 1101 80-81 -0.8533618582066858 1101 82-83 -0.8312918561954703 1101 84-85 -0.8899861926204196 1101 86-87 -1.0561600794982127 1101 88-89 -0.9879066171471607 1101 90-91 -1.1078898053969226 1101 92-93 -1.2135972012610807 1101 94-95 -1.2073902895588198 1101 96-97 -1.2332474857112352 1101 98-99 -1.2079361778918987 1101 100 -1.408057168081374 1102 1 -0.5213144997201198 1102 2 -0.4235478118253795 1102 3 -0.4491190659167046 1102 4 -0.28189936075538924 1102 5 -0.4025610938633122 1102 6 -0.4120638315871119 1102 7 -0.3568795797611699 1102 8 -0.3982447101188171 1102 9 -0.4674126228378128 1102 10-11 -0.5741322054726155 1102 12-13 -0.5634414444106071 1102 14-15 -0.6662451196682255 1102 16-17 -0.7689344780891787 1102 18-19 -0.8151808750917198 1102 20-21 -0.830314757613003 1102 22-23 -0.7284003931822696 1102 24-25 -0.8182050528187474 1102 26-27 -0.7956565308811747 1102 28-29 -0.8895717707287503 1102 30-31 -0.921560088076987 1102 32-33 -0.9518973854856299 1102 34-35 -0.9395188665430467 1102 36-37 -0.9432630178057835 1102 38-39 -1.0774981278836222 1102 40-41 -1.130071883192933 1102 42-43 -1.0880420143404095 1102 44-45 -1.1529432485960953 1102 46-47 -1.226113671189168 1102 48-49 -1.1300561563977212 1102 50-51 -1.1249750213207577 1102 52-53 -1.1347037741870025 1102 54-55 -0.8470966658873564 1102 56-57 -0.889540796531044 1102 58-59 -0.8627197770716037 1102 60-61 -0.8113954926325206 1102 62-63 -0.845442558930813 1102 64-65 -0.9000938250934922 1102 66-67 -0.8312923862970578 1102 68-69 -0.9168687273599616 1102 70-71 -0.9303369338293592 1102 72-73 -0.9170375030247513 1102 74-75 -1.0654174515593517 1102 76-77 -1.0449036409382728 1102 78-79 -1.0166858968699621 1102 80-81 -0.9905331715286358 1102 82-83 -1.0984469750629628 1102 84-85 -1.0725355578944153 1102 86-87 -1.1317260193254945 1102 88-89 -1.191326364818142 1102 90-91 -1.0794465174591323 1102 92-93 -1.0876200081664855 1102 94-95 -1.0963607194315586 1102 96-97 -1.203683457600512 1102 98-99 -1.1947648997010312 1102 100 -1.2969713296658263 1103 1 -0.5017969088804008 1103 2 -0.4450536556353022 1103 3 -0.4928041778633663 1103 4 -0.4318075096267151 1103 5 -0.34103706149751645 1103 6 -0.41962475639715535 1103 7 -0.41913670672837355 1103 8 -0.4276122846073207 1103 9 -0.6192101196651478 1103 10-11 -0.5150160053575163 1103 12-13 -0.6030529862743279 1103 14-15 -0.7662859981760661 1103 16-17 -0.8058070446404528 1103 18-19 -0.6960710484834252 1103 20-21 -0.7583479158397708 1103 22-23 -0.8001611317072488 1103 24-25 -0.7711496302532623 1103 26-27 -0.8027031273037721 1103 28-29 -0.8474850560411085 1103 30-31 -0.8354803988994277 1103 32-33 -0.9247734523707081 1103 34-35 -0.945007369639363 1103 36-37 -0.9168512045120494 1103 38-39 -0.9044292752481695 1103 40-41 -0.9592036717721371 1103 42-43 -1.0757649529643913 1103 44-45 -0.98862748971748 1103 46-47 -0.9295847332722644 1103 48-49 -1.034160418831938 1103 50-51 -1.0263048856089085 1103 52-53 -0.8516282611291004 1103 54-55 -0.6291320027764442 1103 56-57 -0.5712472979561127 1103 58-59 -0.5744610295051089 1103 60-61 -0.6535046919870382 1103 62-63 -0.5680891444271765 1103 64-65 -0.5935372501144514 1103 66-67 -0.6498569113872108 1103 68-69 -0.660626605802598 1103 70-71 -0.6685394241717049 1103 72-73 -0.6360736929068409 1103 74-75 -0.6341667378729312 1103 76-77 -0.6629925461516244 1103 78-79 -0.7194549732233888 1103 80-81 -0.7321047638366984 1103 82-83 -0.6978876736228123 1103 84-85 -0.8246122238400417 1103 86-87 -0.867436490078962 1103 88-89 -0.8528418696713409 1103 90-91 -0.9053794475126473 1103 92-93 -0.9410363991502244 1103 94-95 -0.9086873950882506 1103 96-97 -0.9828298160281541 1103 98-99 -1.0290323948293683 1103 100 -1.2178393783833243 1104 1 -0.4802783870959999 1104 2 -0.5090369363538976 1104 3 -0.4922630931097558 1104 4 -0.3528594718669069 1104 5 -0.3386100446102063 1104 6 -0.36397902004932803 1104 7 -0.3178775703947707 1104 8 -0.41514470546545823 1104 9 -0.5379262726127791 1104 10-11 -0.49646646287201435 1104 12-13 -0.4924021953048552 1104 14-15 -0.6862873952010702 1104 16-17 -0.7337445951789263 1104 18-19 -0.7645055351694836 1104 20-21 -0.7076102494642171 1104 22-23 -0.6582148380431647 1104 24-25 -0.704957929717601 1104 26-27 -0.7657134398195424 1104 28-29 -0.8164605689702569 1104 30-31 -0.8253379422734568 1104 32-33 -0.8435966365703322 1104 34-35 -0.9333826002999288 1104 36-37 -0.9697666415110362 1104 38-39 -0.9171945697464778 1104 40-41 -0.8811311580860064 1104 42-43 -0.8604575857793435 1104 44-45 -0.9771295419572041 1104 46-47 -0.9886968572810062 1104 48-49 -0.930873901152367 1104 50-51 -0.9793514567574775 1104 52-53 -0.8182645832010849 1104 54-55 -0.7636186681916755 1104 56-57 -0.7831736426262381 1104 58-59 -0.8237850091382128 1104 60-61 -0.8564223162474036 1104 62-63 -0.8969595782910389 1104 64-65 -0.822865986653845 1104 66-67 -0.8967143623146043 1104 68-69 -0.8190029323310171 1104 70-71 -0.8691866762864962 1104 72-73 -0.7714352542679634 1104 74-75 -0.8110082828841527 1104 76-77 -0.8808583466008351 1104 78-79 -1.0033107591475314 1104 80-81 -0.9192397570656574 1104 82-83 -0.9331836670487093 1104 84-85 -0.9108290766768974 1104 86-87 -1.0020480754810386 1104 88-89 -1.0020782917343816 1104 90-91 -0.9610746080514367 1104 92-93 -1.006033073686904 1104 94-95 -0.9108042373174925 1104 96-97 -1.0771762513152652 1104 98-99 -1.066918463671211 1104 100 -1.1558951553037247 1105 1 -0.5536039881770805 1105 2 -0.45344773634338864 1105 3 -0.5341880655770481 1105 4 -0.3789623928426522 1105 5 -0.3174072692811123 1105 6 -0.35406943404733227 1105 7 -0.37915825752204313 1105 8 -0.38323761831079395 1105 9 -0.6070664238655041 1105 10-11 -0.5901810018155729 1105 12-13 -0.6165550120026992 1105 14-15 -0.8580674217821809 1105 16-17 -0.7605772424839046 1105 18-19 -0.7621294204231859 1105 20-21 -0.7893818032169051 1105 22-23 -0.8208656707135589 1105 24-25 -0.8286269829506949 1105 26-27 -0.79490508102392 1105 28-29 -0.774470796088238 1105 30-31 -0.7716417903231587 1105 32-33 -0.9260377946084901 1105 34-35 -0.8155936621189213 1105 36-37 -0.823526339674487 1105 38-39 -0.8821915954970265 1105 40-41 -0.8856487722521962 1105 42-43 -0.9424267260500443 1105 44-45 -1.0020885514644462 1105 46-47 -1.0498354015630866 1105 48-49 -1.187136149525685 1105 50-51 -1.1002706232668729 1105 52-53 -0.9680274992874303 1105 54-55 -0.8040112253215526 1105 56-57 -0.8055911022270053 1105 58-59 -0.7118101911168893 1105 60-61 -0.6635638395568435 1105 62-63 -0.8116067813418937 1105 64-65 -0.7671569407150258 1105 66-67 -0.7577555964808056 1105 68-69 -0.827877901573217 1105 70-71 -0.7795032009297742 1105 72-73 -0.765719280358546 1105 74-75 -0.8363588819943502 1105 76-77 -0.7299610748675107 1105 78-79 -0.9024537413123852 1105 80-81 -0.8578564443967842 1105 82-83 -0.9635215091371627 1105 84-85 -0.977791695099409 1105 86-87 -0.9461101875246385 1105 88-89 -0.9255074938471743 1105 90-91 -1.078797352260409 1105 92-93 -1.039290318791334 1105 94-95 -1.1266329181602188 1105 96-97 -1.1566829758265058 1105 98-99 -1.1424721638047508 1105 100 -1.1976499147000865 1106 1 -0.5809583643042657 1106 2 -0.6206695412471284 1106 3 -0.6231740366117435 1106 4 -0.4710550971998515 1106 5 -0.46624050436799536 1106 6 -0.47331099499113805 1106 7 -0.47532675057846774 1106 8 -0.5463445190839664 1106 9 -0.6583031256000069 1106 10-11 -0.6089193211497701 1106 12-13 -0.7227541930119017 1106 14-15 -0.8164317269495243 1106 16-17 -0.8686410456393574 1106 18-19 -0.9163230394535375 1106 20-21 -0.8121409010868561 1106 22-23 -0.8744982253227533 1106 24-25 -0.7984198294578135 1106 26-27 -0.8849715667915277 1106 28-29 -0.963248408770113 1106 30-31 -0.9434966807070708 1106 32-33 -0.9259231628469209 1106 34-35 -1.0012017474748163 1106 36-37 -0.9776442461433348 1106 38-39 -0.9801288517287432 1106 40-41 -1.0242401091391429 1106 42-43 -0.8851318420083274 1106 44-45 -1.0358686510505066 1106 46-47 -0.9642184923414945 1106 48-49 -0.9555384555308066 1106 50-51 -0.9800613805651679 1106 52-53 -0.8599767128056186 1106 54-55 -0.7468695324841761 1106 56-57 -0.7701089253891524 1106 58-59 -0.7465040742745188 1106 60-61 -0.7235920288315754 1106 62-63 -0.7211285369785116 1106 64-65 -0.7168279720882254 1106 66-67 -0.7569847346775873 1106 68-69 -0.789031863050667 1106 70-71 -0.704313759270498 1106 72-73 -0.7755322497273696 1106 74-75 -0.7677718700443847 1106 76-77 -0.7442511585693019 1106 78-79 -0.7721370842348847 1106 80-81 -0.795598446599282 1106 82-83 -0.8679370003131659 1106 84-85 -0.8385625084989137 1106 86-87 -0.8196599076857787 1106 88-89 -0.8351945089106678 1106 90-91 -0.9233585150588333 1106 92-93 -0.9330821111441274 1106 94-95 -1.0376752044270923 1106 96-97 -1.0425621677514059 1106 98-99 -0.9705460087679292 1106 100 -1.2142820839110868 1107 1 -0.5882476599175277 1107 2 -0.5727983049075007 1107 3 -0.6071227288876102 1107 4 -0.543679507239851 1107 5 -0.43248038302124314 1107 6 -0.47438617113293446 1107 7 -0.4895108407485296 1107 8 -0.5292692874029754 1107 9 -0.711452825106079 1107 10-11 -0.6594032351940271 1107 12-13 -0.6902694096640403 1107 14-15 -0.8491235345724988 1107 16-17 -0.8714249798645639 1107 18-19 -0.892708019376883 1107 20-21 -0.8328761210380691 1107 22-23 -0.9286903085302072 1107 24-25 -0.9553540837180492 1107 26-27 -0.9685608411232991 1107 28-29 -0.9660302474784004 1107 30-31 -0.9937706271547384 1107 32-33 -1.0600446760827182 1107 34-35 -0.9902710353100304 1107 36-37 -1.0740482303256798 1107 38-39 -1.0319505053865683 1107 40-41 -0.9913288641026412 1107 42-43 -1.0512597048843588 1107 44-45 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2311 38-39 1.280442948039969 2311 40-41 1.248543169510647 2311 42-43 1.210951781671291 2311 44-45 1.3123979368795204 2311 46-47 1.3164367875731102 2311 48-49 1.3166006911781523 2311 50-51 1.3003643441454713 2311 52-53 1.2380199927415134 2311 54-55 1.1544882577880244 2311 56-57 1.0964887475383307 2311 58-59 1.0534216593364363 2311 60-61 1.013962698529511 2311 62-63 1.054933253467695 2311 64-65 1.0573030320317258 2311 66-67 1.0742562749072135 2311 68-69 1.0832191943050091 2311 70-71 1.03693251125096 2311 72-73 1.0494102175146978 2311 74-75 1.0717592122650146 2311 76-77 1.1031059870323148 2311 78-79 1.1437469611404438 2311 80-81 1.0500951852383587 2311 82-83 1.114031979936172 2311 84-85 1.175353510269634 2311 86-87 1.1557818599191734 2311 88-89 1.2518976928324967 2311 90-91 1.2608268236025175 2311 92-93 1.2830918372084632 2311 94-95 1.243414972916053 2311 96-97 1.2847899333940056 2311 98-99 1.4294222027193122 2311 100 1.4336211823634102 2312 1 0.6138184975626437 2312 2 0.624947089842685 2312 3 0.6445896437122727 2312 4 0.3855229163827403 2312 5 0.4809170464050325 2312 6 0.5427983603959703 2312 7 0.4542150274127792 2312 8 0.5480831327140905 2312 9 0.7271376913125138 2312 10-11 0.68039244227338 2312 12-13 0.825354136424842 2312 14-15 1.051600784244151 2312 16-17 1.022196523826942 2312 18-19 0.9971878637811002 2312 20-21 0.9996060678030219 2312 22-23 1.0431692453554575 2312 24-25 1.069684368848442 2312 26-27 1.0265797487822539 2312 28-29 1.0878115492175269 2312 30-31 1.0855940154435757 2312 32-33 1.132462764713928 2312 34-35 1.1168926157289718 2312 36-37 1.1724055273535399 2312 38-39 1.2068523708327419 2312 40-41 1.2423304757445166 2312 42-43 1.2310361972330952 2312 44-45 1.2285108693188604 2312 46-47 1.3052055121382011 2312 48-49 1.319810129431076 2312 50-51 1.3317619185355554 2312 52-53 1.2221991975141577 2312 54-55 1.1294159535083352 2312 56-57 1.094710083927886 2312 58-59 1.0571998408117764 2312 60-61 1.0293799738153666 2312 62-63 1.0327291415074882 2312 64-65 1.0264551243424833 2312 66-67 1.031259180719708 2312 68-69 1.0426732022324572 2312 70-71 0.9920975700546038 2312 72-73 1.0086980785307702 2312 74-75 1.0647462282231501 2312 76-77 1.138361707981673 2312 78-79 1.1350738579061996 2312 80-81 1.1028389254572026 2312 82-83 1.1337113124895382 2312 84-85 1.1886262932314615 2312 86-87 1.1694522657972186 2312 88-89 1.1434012730018033 2312 90-91 1.1721231652514383 2312 92-93 1.23910752414832 2312 94-95 1.2989653774555379 2312 96-97 1.2740466545641063 2312 98-99 1.0054563864250063 2312 100 1.2428466592462541 2313 1 0.6060050305384372 2313 2 0.6242499465886908 2313 3 0.610792428127823 2313 4 0.5171742122365188 2313 5 0.36505629623754743 2313 6 0.49537304242069524 2313 7 0.5717694429722826 2313 8 0.5781987173530112 2313 9 0.721210585863119 2313 10-11 0.7659961860478148 2313 12-13 0.82507706553438 2313 14-15 0.9739856053124853 2313 16-17 0.9846665652693289 2313 18-19 0.9385373287908223 2313 20-21 0.9955114953747639 2313 22-23 1.0067259119701646 2313 24-25 1.0141344678008508 2313 26-27 1.0284526606005144 2313 28-29 1.0512944267177886 2313 30-31 1.0856097092868353 2313 32-33 1.127271262030952 2313 34-35 1.158458209911167 2313 36-37 1.2068716431795963 2313 38-39 1.1933901389302406 2313 40-41 1.214789655807202 2313 42-43 1.2812195125428758 2313 44-45 1.2876838631289687 2313 46-47 1.2870302699894296 2313 48-49 1.2672361826400191 2313 50-51 1.2742606364533842 2313 52-53 1.1612014068765788 2313 54-55 1.0431869953645716 2313 56-57 0.9794435885913302 2313 58-59 1.0144605892784782 2313 60-61 0.9467138724223361 2313 62-63 0.9694770771839245 2313 64-65 1.0185281094833272 2313 66-67 0.9870871388748199 2313 68-69 1.0094194985445029 2313 70-71 1.0098516916151326 2313 72-73 0.9851254092156054 2313 74-75 0.9559153749599645 2313 76-77 0.7742346329101224 2313 78-79 0.952712166875834 2313 80-81 0.9766255979679421 2313 82-83 0.9890888411028271 2313 84-85 1.0866902635216427 2313 86-87 1.0989143015192937 2313 88-89 1.127624280418157 2313 90-91 1.1059465835010123 2313 92-93 1.1909393691797199 2313 94-95 1.2002022796662715 2313 96-97 1.2187837456324537 2313 98-99 1.3076308857810766 2313 100 1.4189553798144665 2314 1 0.4948738108989694 2314 2 0.4973100706490463 2314 3 0.52842840228314 2314 4 0.40765006045150187 2314 5 0.43624573106963993 2314 6 0.39556153909679637 2314 7 0.42829582872404615 2314 8 0.40791910697662104 2314 9 0.5799217035159501 2314 10-11 0.5999058508198303 2314 12-13 0.6313145390197761 2314 14-15 0.8104741673522895 2314 16-17 0.7895871785188717 2314 18-19 0.7883453931264981 2314 20-21 0.7993889202053666 2314 22-23 0.8398154353676546 2314 24-25 0.8082317683555829 2314 26-27 0.8784143187476587 2314 28-29 0.8826286618684662 2314 30-31 0.8851551696160413 2314 32-33 0.8593197864986664 2314 34-35 0.9081915450327713 2314 36-37 0.8929618729452784 2314 38-39 0.9384092373513298 2314 40-41 0.990624338370246 2314 42-43 1.058498441197166 2314 44-45 1.0789667673682715 2314 46-47 1.1210267835431722 2314 48-49 1.01355143572205 2314 50-51 1.0830475465408043 2314 52-53 1.0497392992536732 2314 54-55 0.9648289465960502 2314 56-57 0.8578276533057547 2314 58-59 0.8515627384626967 2314 60-61 0.8520155714200683 2314 62-63 0.8505481523583782 2314 64-65 0.8879825260383356 2314 66-67 0.8416986673262485 2314 68-69 0.8983141572914093 2314 70-71 0.8930469438396997 2314 72-73 0.8925663165078177 2314 74-75 0.8682029199992485 2314 76-77 0.8715160725989861 2314 78-79 0.8971648266810277 2314 80-81 0.8864797865868219 2314 82-83 0.9415561202762177 2314 84-85 0.970327153479527 2314 86-87 0.9863687693152343 2314 88-89 1.0572606326329108 2314 90-91 1.1660157346761828 2314 92-93 1.1824094890720254 2314 94-95 1.135079492311295 2314 96-97 1.1771952872896065 2314 98-99 1.2076895176644378 2314 100 1.3296059951182393 2315 1 0.5047507996401137 2315 2 0.5063304967472924 2315 3 0.5351729501592217 2315 4 0.2856607560889515 2315 5 0.3642253324436382 2315 6 0.41976242806589426 2315 7 0.4294732824534151 2315 8 0.44901390982342804 2315 9 0.5777482692217433 2315 10-11 0.4881129558869475 2315 12-13 0.5566603226406315 2315 14-15 0.7346450611338682 2315 16-17 0.7430215553657078 2315 18-19 0.6271705418153743 2315 20-21 0.7899272118620289 2315 22-23 0.6720985648852249 2315 24-25 0.699321877096537 2315 26-27 0.8381889119536865 2315 28-29 0.8593010915644612 2315 30-31 0.862598157634423 2315 32-33 0.8777696187169468 2315 34-35 0.9458326715943528 2315 36-37 0.9672886791635094 2315 38-39 1.046467098143431 2315 40-41 0.9875514160499961 2315 42-43 1.070933303498677 2315 44-45 1.1540876350102778 2315 46-47 1.155811410819254 2315 48-49 1.1613945145551838 2315 50-51 1.094688621954873 2315 52-53 1.1044218354973054 2315 54-55 0.9967861987029636 2315 56-57 0.9519205166046092 2315 58-59 0.9527696894916744 2315 60-61 0.9306566973860555 2315 62-63 0.9887323918067636 2315 64-65 0.9951376974316446 2315 66-67 1.0297641097879833 2315 68-69 0.9980604820038153 2315 70-71 0.9284978986975077 2315 72-73 0.6901139840174153 2315 74-75 0.8845749542810353 2315 76-77 0.9061761720589345 2315 78-79 0.9822943302121487 2315 80-81 0.6823412845552888 2315 82-83 0.7247104345205884 2315 84-85 0.9368917186906707 2315 86-87 1.0180864163539098 2315 88-89 1.003860902270393 2315 90-91 1.053682580173021 2315 92-93 1.116076031331854 2315 94-95 1.1407597093837225 2315 96-97 1.0359891427459864 2315 98-99 0.48823757946403745 2315 100 0.9417115264351814 2316 1 0.3509509505424866 2316 2 0.26791828828171305 2316 3 0.30499581593194947 2316 4 0.20086307933544134 2316 5 0.1648961693408637 2316 6 0.17864998804010668 2316 7 0.14676510248533248 2316 8 0.23053505782512929 2316 9 0.2431430200823428 2316 10-11 0.22203973874101735 2316 12-13 0.01023387836887224 2316 14-15 -0.01214922272390595 2316 16-17 0.2368811017918162 2316 18-19 0.2862713860741266 2316 20-21 0.29511175351789376 2316 22-23 0.2493286448087133 2316 24-25 0.3231031688738284 2316 26-27 0.4297831395616569 2316 28-29 0.3738172250868814 2316 30-31 0.29001314488266416 2316 32-33 0.4536153108062777 2316 34-35 0.4513645798924202 2316 36-37 0.5039958736313466 2316 38-39 0.6015744166979218 2316 40-41 0.6606816629472263 2316 42-43 0.5492549502246007 2316 44-45 0.42292652415330423 2316 46-47 0.36063678059628046 2316 48-49 0.3320465458383808 2316 50-51 0.4126418286754756 2316 52-53 0.5665481685334868 2316 54-55 0.25849714014838554 2316 56-57 0.1715644115291255 2316 58-59 0.2025702268895202 2316 60-61 0.31661322100946165 2316 62-63 0.4074972405221686 2316 64-65 0.3607658707003196 2316 66-67 0.33505196795540115 2316 68-69 0.3090046962791675 2316 70-71 0.05367597418361214 2316 72-73 0.02728337343763343 2316 74-75 0.20605250190978808 2316 76-77 0.3690061920006755 2316 78-79 0.4133800952185638 2316 80-81 0.5435711795375902 2316 82-83 0.6517547681875868 2316 84-85 0.714971327969689 2316 86-87 0.742316653229107 2316 88-89 0.6771547972851941 2316 90-91 0.5301952004148838 2316 92-93 0.5650328291317024 2316 94-95 0.7225598228765158 2316 96-97 0.6774553975260176 2316 98-99 0.5819415322695747 2316 100 0.5013861699004849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 26.0 1 521.0 2 186.0 3 199.0 4 124.0 5 111.0 6 146.0 7 495.0 8 1634.0 9 3124.0 10 4941.0 11 7902.0 12 11598.0 13 15011.0 14 18512.0 15 22624.0 16 28016.0 17 36077.0 18 45148.0 19 55515.0 20 68857.0 21 85718.0 22 95649.0 23 93980.0 24 88791.0 25 89324.0 26 96539.0 27 110169.0 28 128606.0 29 154588.0 30 187706.0 31 232294.0 32 297132.0 33 390524.0 34 563558.0 35 783882.0 36 1186002.0 37 1663174.0 38 1461628.0 39 445072.0 40 537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01894416658098 20.579197658966045 12.829209375704998 22.57264879874798 2 15.491855843202712 19.971744617465408 41.751202826766956 22.785196712564925 3 19.266895477880894 27.112783631796855 28.62534494264084 24.994975947681414 4 12.20616717776407 16.54572576283751 39.9463244471575 31.30178261224092 5 12.68321689734567 38.58064883094146 34.64128861307138 14.094845658641491 6 29.01973306387497 38.432557401064656 18.396081363991303 14.151628171069072 7 26.893866326928855 32.40869920194512 23.19958360711551 17.497850864010513 8 26.003771683758387 35.20093376086637 20.06574241213058 18.729552143244657 9 26.460670437311467 15.633905870324805 22.042821711388335 35.8626019809754 10-11 25.656083041748083 26.369750358961163 27.226866406251382 20.74730019303937 12-13 25.86711729921689 24.27890667390285 27.903025398598885 21.95095062828138 14-15 23.132768906613517 25.343497262536307 26.20737224136883 25.31636158948134 16-17 21.28220438637534 28.062866148348014 27.38796428451316 23.26696518076348 18-19 20.66552429606555 27.95810243125273 30.049110292652127 21.327262980029598 20-21 22.343734824692486 26.970351601586096 29.557294939591433 21.12861863412998 22-23 22.579642222937917 26.84476252934299 29.109178049325372 21.46641719839372 24-25 21.996227098683732 26.704676946062385 30.070312624467682 21.228783330786204 26-27 21.839325087648483 26.970756657812377 29.218714053855134 21.971204200684006 28-29 21.00335863842357 27.477619058548687 29.73705170632202 21.781970596705722 30-31 22.02812980682146 27.049910405420636 29.61260448521616 21.30935530254175 32-33 22.233802486467553 27.306375201977694 28.77888286922325 21.680939442331503 34-35 22.28042979825865 27.141560946866576 29.490069652600848 21.08793960227393 36-37 21.91223933223452 26.729908274355214 29.188098436649852 22.169753956760413 38-39 22.0192397618204 27.116800111401453 29.556344321343612 21.307615805434537 40-41 21.820097403009303 26.180367572887775 30.057115916189865 21.942419107913057 42-43 21.753889172208915 26.427894568442362 29.26371426612252 22.554501993226207 44-45 21.84600319894695 27.25618593504398 29.17221375462205 21.72559711138702 46-47 21.92888443326446 27.1071195420937 28.528819428521313 22.435176596120527 48-49 22.508799242710378 26.980520093669742 28.55151835624717 21.959162307372715 50-51 22.143073915360468 26.915729210941596 28.523425874834267 22.41777099886367 52-53 21.810545831966873 27.5590869494065 28.07461849160977 22.555748727016855 54-55 21.70476796351529 27.359331680159194 28.197640863065487 22.73825949326003 56-57 22.567560414003744 26.93570815617304 27.80567051823528 22.691060911587936 58-59 21.802414193875002 27.30701017543572 28.481257806210454 22.409317824478826 60-61 21.827660880135006 27.96320969588255 27.930231185904624 22.27889823807782 62-63 22.099613452522544 27.26954729547763 27.66239679262602 22.968442459373804 64-65 21.43998764152589 27.401187083836763 27.99210318051326 23.166722094124083 66-67 21.965936923441433 27.012061608948233 27.808884914151875 23.21311655345846 68-69 21.54881026241286 27.155714615946486 28.050479477847674 23.244995643792983 70-71 21.678404012159707 26.804465640453035 28.162830513045833 23.35429983434143 72-73 22.24865717152823 26.91456671218354 28.30437669629641 22.532399419991822 74-75 22.405393393257302 27.32828506701675 27.74927880223816 22.51704273748779 76-77 22.176293203679442 27.12805386817746 27.555027348240557 23.140625579902537 78-79 22.038099897734075 27.181193124463505 27.569292656372102 23.211414321430315 80-81 21.69602114046093 27.617630221078173 27.961834207832815 22.724514430628084 82-83 22.617970998484587 26.741532698528008 28.916059260061722 21.724437042925683 84-85 22.672858983064486 27.1677261969711 27.772021024973657 22.387393794990754 86-87 21.57558400579625 27.86397811302097 28.09082482687657 22.469613054306212 88-89 22.534382149149614 26.747983583208583 27.922228226772816 22.795406040868983 90-91 22.3662518562855 26.836395043989132 27.96356629618056 22.833786803544807 92-93 22.76378304967858 27.01400500790388 27.860470218065142 22.3617417243524 94-95 21.71710779382372 26.99161009007823 28.461099682599926 22.83018243349813 96-97 21.984461202838546 26.995416155739417 28.050432613099247 22.96969002832279 98-99 21.546563947846884 27.462907857782003 27.72795035923184 23.26257783513927 100 22.369290196034463 26.828096168473625 26.99652022746866 23.806093408023248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1726.0 1 1172.5 2 1293.5 3 1887.0 4 1425.5 5 1216.5 6 1729.5 7 3017.5 8 4972.0 9 6042.5 10 5686.5 11 5388.0 12 5946.0 13 6802.0 14 7869.0 15 9226.0 16 10505.0 17 11791.0 18 12878.0 19 13881.0 20 15214.0 21 16534.0 22 18131.0 23 20345.5 24 22900.0 25 26240.5 26 31560.0 27 37621.5 28 44777.5 29 59017.0 30 80992.0 31 102300.0 32 131722.5 33 165734.5 34 194413.0 35 221561.5 36 245772.5 37 281615.5 38 295541.5 39 282777.0 40 265111.0 41 245246.0 42 238820.0 43 246545.0 44 264219.0 45 300309.0 46 334564.0 47 359885.0 48 384884.5 49 380020.5 50 356552.0 51 356626.0 52 352514.0 53 330099.5 54 305987.0 55 261736.0 56 226372.5 57 185002.5 58 143809.0 59 135280.5 60 112347.0 61 77346.5 62 57548.0 63 48878.5 64 36407.5 65 18568.0 66 10592.0 67 7107.5 68 5262.0 69 4127.5 70 3274.5 71 2633.0 72 2091.0 73 1855.5 74 1542.5 75 1147.0 76 805.0 77 530.0 78 390.5 79 302.0 80 219.5 81 159.5 82 126.5 83 99.0 84 71.5 85 53.5 86 45.0 87 32.0 88 24.0 89 21.0 90 18.5 91 14.0 92 12.5 93 10.0 94 8.0 95 8.0 96 4.5 97 3.5 98 4.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057258212949110626 2 0.13464469939733165 3 0.016576919265093845 4 0.016411739998395403 5 0.018865831960772284 6 0.05102859489076931 7 0.05656210032516718 8 0.048279539952145206 9 0.03951324029807778 10-11 0.03864014988838601 12-13 0.040126763288672006 14-15 0.018346697122577173 16-17 0.0170488600270894 18-19 0.017096054103288957 20-21 0.0207535950087545 22-23 0.025378614476310932 24-25 0.024245956647521605 26-27 0.01877734306789812 28-29 0.017951446734405897 30-31 0.015986993312599403 32-33 0.022794738804385276 34-35 0.022930421773458998 36-37 0.02296581733060866 38-39 0.021030860206426888 40-41 0.024570415921393546 42-43 0.031773411801350696 44-45 0.04102345073646356 46-47 0.046610049506585934 48-49 0.03917108324563101 50-51 0.02721328418856865 52-53 0.0391297884289564 54-55 0.035985483102161014 56-57 0.03780835429536884 58-59 0.04270473970107272 60-61 0.022889126956784384 62-63 0.025555592262059265 64-65 0.02480048704286638 66-67 0.03164952735132686 68-69 0.03830389209546418 70-71 0.04005597217437267 72-73 0.04354243455361483 74-75 0.026381488595551485 76-77 0.019992590530036672 78-79 0.01951475050851617 80-81 0.02396279219032427 82-83 0.020098777201485667 84-85 0.012406142780958133 86-87 0.014099230264617185 88-89 0.017833461543907008 90-91 0.020387840918207948 92-93 0.019296477906093228 94-95 0.02300121288775833 96-97 0.024487826288044325 98-99 0.026924220471846373 100 0.028139467933984925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8475640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.47457501456225 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90101722422364 49.5816040371935 2 14.449952016866192 18.633090305365283 3 4.575609435365275 8.850314213333919 4 1.8249781220824965 4.706587553285715 5 0.8791410026063138 2.834112126045912 6 0.4344626545248155 1.6807077006111635 7 0.24825605200716702 1.120434241356861 8 0.14758448116732226 0.7612357361606215 9 0.10565964043015136 0.6131124371632921 >10 0.3830516460593497 4.226832454271072 >50 0.025662578128820526 1.1469199342135832 >100 0.02190632820443345 2.7633245351094327 >500 0.0017258879233898704 0.7621419284293521 >1k 8.747244104356362E-4 0.8726824801538265 >5k 4.728240009333265E-5 0.21967582168050184 >10k+ 7.092360013999897E-5 1.2272244956260232 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35497 0.41881203071390477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28335 0.3343110372786008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16681 0.19681109627119603 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1798519049888857E-5 7 0.0 0.0 0.0 1.1798519049888857E-5 1.1798519049888857E-5 8 0.0 0.0 0.0 1.1798519049888857E-5 1.1798519049888857E-5 9 0.0 0.0 0.0 3.5395557149666574E-5 1.1798519049888857E-5 10-11 0.0 0.0 0.0 3.5395557149666574E-5 1.1798519049888857E-5 12-13 0.0 0.0 0.0 5.3093335724499865E-5 1.1798519049888857E-5 14-15 0.0 0.0 0.0 5.899259524944429E-5 3.5395557149666574E-5 16-17 0.0 0.0 0.0 7.669037382427758E-5 3.5395557149666574E-5 18-19 0.0 0.0 0.0 1.2978370954877744E-4 3.5395557149666574E-5 20-21 0.0 0.0 0.0 2.1237334289799943E-4 3.5395557149666574E-5 22-23 0.0 0.0 0.0 4.8373928104544317E-4 3.5395557149666574E-5 24-25 0.0 0.0 0.0 0.0010559674549650527 3.5395557149666574E-5 26-27 0.0 0.0 0.0 0.002035244536105828 3.5395557149666574E-5 28-29 0.0 0.0 0.0 0.005610195808222152 3.5395557149666574E-5 30-31 0.0 0.0 0.0 0.019272880867993447 3.5395557149666574E-5 32-33 0.0 0.0 0.0 0.05190758455998603 3.5395557149666574E-5 34-35 0.0 0.0 0.0 0.10137877493617001 3.5395557149666574E-5 36-37 0.0 0.0 0.0 0.18337848233289758 3.5395557149666574E-5 38-39 0.0 0.0 0.0 0.3013695720913111 3.5395557149666574E-5 40-41 0.0 0.0 0.0 0.42622150067723497 3.5395557149666574E-5 42-43 0.0 0.0 0.0 0.5478819298601639 3.5395557149666574E-5 44-45 0.0 0.0 0.0 0.690661708142394 3.5395557149666574E-5 46-47 0.0 0.0 0.0 0.8581652830936661 3.5395557149666574E-5 48-49 0.0 0.0 0.0 1.0438739729389166 3.5395557149666574E-5 50-51 0.0 0.0 0.0 1.2330632259038845 3.5395557149666574E-5 52-53 0.0 0.0 0.0 1.4144418592578258 4.1294816674611E-5 54-55 0.0 0.0 0.0 1.6110228844075491 4.719407619955543E-5 56-57 0.0 0.0 0.0 1.8415482488638026 4.719407619955543E-5 58-59 0.0 0.0 0.0 2.1033101925046367 4.719407619955543E-5 60-61 0.0 0.0 0.0 2.368015866648418 4.719407619955543E-5 62-63 0.0 0.0 0.0 2.6321788089159046 4.719407619955543E-5 64-65 0.0 1.1798519049888857E-5 0.0 2.9019401484725638 4.719407619955543E-5 66-67 0.0 1.1798519049888857E-5 0.0 3.2034572020519985 5.899259524944429E-5 68-69 0.0 1.1798519049888857E-5 0.0 3.5333496939464157 5.899259524944429E-5 70-71 5.899259524944429E-6 1.1798519049888857E-5 0.0 3.887246272847832 5.899259524944429E-5 72-73 1.1798519049888857E-5 1.1798519049888857E-5 0.0 4.2403051568967065 7.079111429933315E-5 74-75 1.1798519049888857E-5 1.1798519049888857E-5 0.0 4.579530277359586 7.079111429933315E-5 76-77 1.1798519049888857E-5 1.1798519049888857E-5 0.0 4.9584751122039155 7.079111429933315E-5 78-79 1.1798519049888857E-5 1.1798519049888857E-5 0.0 5.371317092278577 7.079111429933315E-5 80-81 1.1798519049888857E-5 1.1798519049888857E-5 0.0 5.805661873321661 7.079111429933315E-5 82-83 1.1798519049888857E-5 3.5395557149666574E-5 0.0 6.247840871013871 8.2589633349222E-5 84-85 1.1798519049888857E-5 3.5395557149666574E-5 0.0 6.67153748861443 8.2589633349222E-5 86-87 1.1798519049888857E-5 3.5395557149666574E-5 0.0 7.1175981990740524 8.2589633349222E-5 88 1.1798519049888857E-5 3.5395557149666574E-5 0.0 7.474715773676088 8.2589633349222E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4910 0.0 38.914833 2 GTACTAG 8360 0.0 36.53521 1 ACCTGGG 5285 0.0 35.48979 3 GCATCGT 7910 0.0 35.29963 8 GGTATCA 26555 0.0 33.22983 1 TAGGCAT 10000 0.0 32.012913 5 GGGCACC 8560 0.0 31.954723 94 GTATCAA 40760 0.0 31.925238 1 CTAGGCA 10960 0.0 29.980904 4 GTACCTG 6735 0.0 28.85929 1 CTGTGCG 10775 0.0 28.007399 9 CAACGCA 44790 0.0 27.80219 5 GTACATG 41865 0.0 27.777609 1 TAACGCC 3865 0.0 27.487616 4 CTAACGC 3920 0.0 27.461544 3 ATCAACG 45505 0.0 27.334194 3 CATCGTC 10665 0.0 27.018091 9 TACATGG 42505 0.0 27.01596 2 CCTGGGG 5255 0.0 26.836548 4 TAGTACT 11660 0.0 26.729637 4 >>END_MODULE