FastQCFastQC Report
Mon 6 Jun 2016
ERR1211202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8475640
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319290.37671491474390134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271020.3197634632900878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164670.19428621319451983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48100.037.7158282
GTACTAG84150.034.808231
GGTATCA268250.033.932331
GGGCACC88400.033.61184394
ACCTGGG54150.032.5516623
GTATCAA406050.031.0894341
TAGGCAT102000.031.0489065
CTGTGCG103950.030.9636889
TAGTACT113050.029.1465954
GCATCGT96450.028.4508118
ACTGTGC119500.028.2319328
CTAGGCA117450.027.494394
CCTGGGG49450.027.280734
GTACTGT123650.026.7905466
CAACGCA451100.026.7386595
ATCAACG456050.026.468113
GTACCTG70300.026.4173851
GTACATG420750.026.3603921
GAGTTGC93200.026.23100794
TACATGG422800.025.8668062
Produced by FastQC (version 0.11.5)