##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8475640 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20608673799265 33.0 31.0 34.0 30.0 34.0 2 32.34936441377878 34.0 31.0 34.0 31.0 34.0 3 32.42715594338598 34.0 31.0 34.0 31.0 34.0 4 36.02175410942419 37.0 35.0 37.0 35.0 37.0 5 35.92134517275392 37.0 35.0 37.0 35.0 37.0 6 35.9520999004205 37.0 35.0 37.0 35.0 37.0 7 35.8960858413052 37.0 35.0 37.0 35.0 37.0 8 35.88930629427394 37.0 35.0 37.0 35.0 37.0 9 37.50132426577816 39.0 37.0 39.0 35.0 39.0 10-11 37.484152229212185 39.0 37.0 39.0 35.0 39.0 12-13 37.3761760763789 39.0 37.0 39.0 34.0 39.0 14-15 38.59894314765611 40.0 38.0 41.0 34.0 41.0 16-17 38.5648309744161 40.0 38.0 41.0 34.0 41.0 18-19 38.605418351888474 40.0 38.0 41.0 34.0 41.0 20-21 38.576675448697685 40.0 38.0 41.0 34.0 41.0 22-23 38.44205328447173 40.0 38.0 41.0 34.0 41.0 24-25 38.37457106483994 40.0 38.0 41.0 34.0 41.0 26-27 38.23146830209872 40.0 38.0 41.0 34.0 41.0 28-29 38.21585968729205 40.0 38.0 41.0 34.0 41.0 30-31 38.21014165301972 40.0 37.5 41.0 34.0 41.0 32-33 38.0642969144513 40.0 37.0 41.0 33.5 41.0 34-35 37.91892417563747 40.0 37.0 41.0 33.0 41.0 36-37 37.771784431618144 40.0 37.0 41.0 33.0 41.0 38-39 37.6719140973425 40.0 37.0 41.0 33.0 41.0 40-41 37.56554667258166 40.0 36.0 41.0 33.0 41.0 42-43 37.40468548687768 40.0 36.0 41.0 32.0 41.0 44-45 37.22156014177101 40.0 36.0 41.0 31.5 41.0 46-47 36.94692801959498 39.0 35.5 41.0 31.0 41.0 48-49 36.666006873817196 39.0 35.0 41.0 30.5 41.0 50-51 36.437712373342904 39.0 35.0 41.0 30.0 41.0 52-53 36.22127243488397 39.0 35.0 40.0 29.5 41.0 54-55 35.97619796263173 38.0 35.0 40.0 29.0 41.0 56-57 35.69343553997102 38.0 35.0 40.0 28.5 41.0 58-59 35.40309068105771 37.5 34.0 40.0 28.0 41.0 60-61 35.141511142521395 37.0 34.0 40.0 28.0 41.0 62-63 34.835053577075 36.5 34.0 40.0 27.0 41.0 64-65 34.47159836897272 36.0 34.0 39.0 26.0 41.0 66-67 34.17496407350949 35.5 33.0 39.0 26.0 40.5 68-69 33.86239699892869 35.0 33.0 38.5 26.0 40.0 70-71 33.57905497401966 35.0 33.0 38.0 26.0 40.0 72-73 33.267006326365916 35.0 33.0 37.0 25.5 40.0 74-75 32.95031578736237 35.0 32.0 37.0 25.0 39.0 76-77 31.96387293466924 34.0 30.5 36.0 24.0 38.5 78-79 32.457321570996406 35.0 32.0 36.0 25.0 38.5 80-81 32.39538223662166 35.0 32.0 35.5 25.0 37.5 82-83 32.156673301367206 35.0 32.0 35.0 25.0 37.0 84-85 31.918275611045303 34.5 32.0 35.0 24.0 37.0 86-87 31.69808498237301 34.0 31.0 35.0 24.0 36.0 88-89 31.48661121755997 34.0 31.0 35.0 24.0 36.0 90-91 31.274164192910504 34.0 31.0 35.0 23.0 35.5 92-93 31.092003199758366 34.0 31.0 35.0 22.5 35.0 94-95 30.913580980315352 34.0 31.0 35.0 20.0 35.0 96-97 30.754836035980766 34.0 30.0 35.0 20.0 35.0 98-99 30.59683675805013 34.0 30.0 35.0 20.0 35.0 100 28.799705037023752 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3280700847816931 1101 2 -0.33149802849427346 1101 3 -0.40396010099286883 1101 4 -0.17436090649682257 1101 5 -0.24711747781109494 1101 6 -0.3541092134881083 1101 7 -0.29024885942377665 1101 8 -0.3017904475443416 1101 9 -0.4608283002799425 1101 10-11 -0.45549419123695856 1101 12-13 -0.4427053721680494 1101 14-15 -0.683542347663348 1101 16-17 -0.565325220577229 1101 18-19 -0.6394173491875392 1101 20-21 -0.6861931856976256 1101 22-23 -0.7265910139697738 1101 24-25 -0.7530424626659666 1101 26-27 -0.8201398081153854 1101 28-29 -0.8459761828926773 1101 30-31 -0.6540891265360997 1101 32-33 -0.6336483615842283 1101 34-35 -0.6878033198663687 1101 36-37 -0.6711333518534275 1101 38-39 -0.6645232279771776 1101 40-41 -0.7174677172832986 1101 42-43 -0.7811051408428682 1101 44-45 -0.8071672349870482 1101 46-47 -0.8059392879606406 1101 48-49 -0.7351358532848238 1101 50-51 -0.7824061231147184 1101 52-53 -0.8052508130589118 1101 54-55 -0.6744949138658782 1101 56-57 -0.7380986442638218 1101 58-59 -0.7559446795455713 1101 60-61 -0.7660908951753669 1101 62-63 -0.8748369787442343 1101 64-65 -0.7156196798065437 1101 66-67 -0.8222208990649236 1101 68-69 -0.8601223645641767 1101 70-71 -0.9100814031050746 1101 72-73 -0.9670890557233847 1101 74-75 -0.9899274339414745 1101 76-77 -0.9621162638366698 1101 78-79 -0.8151837762126135 1101 80-81 -0.7382405752562207 1101 82-83 -0.6984272941475069 1101 84-85 -0.7166994678044496 1101 86-87 -0.891179692180252 1101 88-89 -0.8274640087556691 1101 90-91 -0.8015510450854286 1101 92-93 -0.8782512106719764 1101 94-95 -0.8648643060726897 1101 96-97 -0.9488780325619999 1101 98-99 -1.0053096029404784 1101 100 -0.8700969968932668 1102 1 -0.410887910099202 1102 2 -0.3383000701958494 1102 3 -0.35279768656999977 1102 4 -0.2466268761367374 1102 5 -0.31477274622966434 1102 6 -0.355627422856692 1102 7 -0.33489164703635765 1102 8 -0.3885257792183765 1102 9 -0.5019279813189641 1102 10-11 -0.5256899152959491 1102 12-13 -0.5449955871361354 1102 14-15 -0.6140174907127331 1102 16-17 -0.6574890465866261 1102 18-19 -0.6470987121955858 1102 20-21 -0.7043141800829957 1102 22-23 -0.7068230840640979 1102 24-25 -0.7207295930271798 1102 26-27 -0.7626613905560902 1102 28-29 -0.749129256350308 1102 30-31 -0.6927298755698672 1102 32-33 -0.6300607458773158 1102 34-35 -0.6601057477659182 1102 36-37 -0.7611253448935713 1102 38-39 -0.812841985976199 1102 40-41 -0.6990046632307099 1102 42-43 -0.7054519978549507 1102 44-45 -0.7425844629785203 1102 46-47 -0.6462404199134824 1102 48-49 -0.7626403020024739 1102 50-51 -0.7732049210460801 1102 52-53 -0.7440275877032505 1102 54-55 -0.7887840241565058 1102 56-57 -0.8074739989292041 1102 58-59 -0.7560698340369569 1102 60-61 -0.7744514689245392 1102 62-63 -0.7141474433674873 1102 64-65 -0.6171317217179322 1102 66-67 -0.8090921685218149 1102 68-69 -0.828488022562091 1102 70-71 -0.8470166257751615 1102 72-73 -0.8829462322478179 1102 74-75 -1.0501596016221448 1102 76-77 -0.7613079853915927 1102 78-79 -0.5940318279255692 1102 80-81 -0.5589654954427878 1102 82-83 -0.6023950965078271 1102 84-85 -0.7055590298271603 1102 86-87 -0.6066872558541263 1102 88-89 -0.6796055265258971 1102 90-91 -0.7333774662964068 1102 92-93 -0.8024398314206884 1102 94-95 -0.8482805894283132 1102 96-97 -1.0104165496496123 1102 98-99 -0.9620532831265969 1102 100 -0.9096800689798137 1103 1 -0.32454292156272047 1103 2 -0.29750471768649334 1103 3 -0.3243162965460229 1103 4 -0.20392397149598906 1103 5 -0.23769386891370203 1103 6 -0.3087766438583728 1103 7 -0.3460311705864143 1103 8 -0.33790699623946807 1103 9 -0.45456616518861637 1103 10-11 -0.4539621358117003 1103 12-13 -0.45314154840036736 1103 14-15 -0.6078427422692485 1103 16-17 -0.6030457411479517 1103 18-19 -0.6016263741838728 1103 20-21 -0.6344341058062426 1103 22-23 -0.6491852805482878 1103 24-25 -0.7375443565799529 1103 26-27 -0.6601907473921571 1103 28-29 -0.7252862864969671 1103 30-31 -0.7071312284729387 1103 32-33 -0.5757403511631836 1103 34-35 -0.6634204045574208 1103 36-37 -0.6941820652549993 1103 38-39 -0.6622723226599163 1103 40-41 -0.7402859593739421 1103 42-43 -0.656846654317718 1103 44-45 -0.7017834669888501 1103 46-47 -0.7367227303360977 1103 48-49 -0.8216578388339926 1103 50-51 -0.8045587123891593 1103 52-53 -0.8079667972117548 1103 54-55 -0.8009474628423092 1103 56-57 -0.819969073435324 1103 58-59 -0.8085657389807466 1103 60-61 -0.8300023468892377 1103 62-63 -0.7842712936803693 1103 64-65 -0.7256124668789781 1103 66-67 -0.8657578337418954 1103 68-69 -0.7524056239561361 1103 70-71 -0.8811071424764165 1103 72-73 -0.8570909665245949 1103 74-75 -0.8729951628223809 1103 76-77 -0.7641385022315603 1103 78-79 -0.546063193552417 1103 80-81 -0.5511526594314091 1103 82-83 -0.6905136068424902 1103 84-85 -0.8034982558105419 1103 86-87 -0.6948442850338559 1103 88-89 -0.7264042404669162 1103 90-91 -0.6916781249323982 1103 92-93 -0.8190620751267694 1103 94-95 -0.882755290468431 1103 96-97 -0.9497529574369743 1103 98-99 -0.7992790125399196 1103 100 -1.0414830032585947 1104 1 -0.35593986259078036 1104 2 -0.303358644834276 1104 3 -0.3772195754005452 1104 4 -0.2541578621868865 1104 5 -0.27110006162715905 1104 6 -0.34968269941625607 1104 7 -0.2995281523722255 1104 8 -0.32217986416900857 1104 9 -0.49234182121386283 1104 10-11 -0.4713424399549524 1104 12-13 -0.578148363156501 1104 14-15 -0.7227563525005749 1104 16-17 -0.6299855219014106 1104 18-19 -0.6877784180015212 1104 20-21 -0.7493850509199902 1104 22-23 -0.6448970092047972 1104 24-25 -0.7530146188083222 1104 26-27 -0.7564725562129198 1104 28-29 -0.8061177955063172 1104 30-31 -0.8385241752967119 1104 32-33 -0.7982851270365288 1104 34-35 -0.7484755823988181 1104 36-37 -0.6861409111492023 1104 38-39 -0.7288546412313224 1104 40-41 -0.7323316589909226 1104 42-43 -0.6438153606133596 1104 44-45 -0.8133800549024031 1104 46-47 -0.8606750648340693 1104 48-49 -0.7911084917048399 1104 50-51 -0.7351087926208635 1104 52-53 -0.727631440308862 1104 54-55 -0.7377517510998857 1104 56-57 -0.7116334323777451 1104 58-59 -0.7232166317585396 1104 60-61 -0.7742357518738814 1104 62-63 -0.8031804092014241 1104 64-65 -0.8909473305724944 1104 66-67 -0.9077859406184743 1104 68-69 -0.9103611823156115 1104 70-71 -0.9024709985517987 1104 72-73 -0.8647267803121395 1104 74-75 -0.8895485840743262 1104 76-77 -0.8248396604441091 1104 78-79 -0.731486874169164 1104 80-81 -0.7143902475061097 1104 82-83 -0.7164527284231781 1104 84-85 -0.6682235825901408 1104 86-87 -0.7953083320669627 1104 88-89 -0.7368687029248022 1104 90-91 -0.7848408921474181 1104 92-93 -0.7827379191360393 1104 94-95 -0.9074879506828353 1104 96-97 -0.9163726814476156 1104 98-99 -0.9682182971240643 1104 100 -1.0161436748672656 1105 1 -0.36759776577278913 1105 2 -0.3567486123365171 1105 3 -0.4022751385211265 1105 4 -0.1508877007970213 1105 5 -0.2703907409584758 1105 6 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68-69 -0.7264567094744478 1105 70-71 -0.8370927552462533 1105 72-73 -0.771424037552471 1105 74-75 -0.7995600192074122 1105 76-77 -0.789898599954963 1105 78-79 -0.7444526308827264 1105 80-81 -0.7108488202326839 1105 82-83 -0.6577259341385115 1105 84-85 -0.7262127869007742 1105 86-87 -0.7028176350573787 1105 88-89 -0.7281980889075292 1105 90-91 -0.7199707995594729 1105 92-93 -0.7453602236975918 1105 94-95 -0.7326122360865526 1105 96-97 -0.8757234111540342 1105 98-99 -0.8950948613921419 1105 100 -1.0155024394269532 1106 1 -0.4357921189696974 1106 2 -0.41555369216208504 1106 3 -0.41156505346403094 1106 4 -0.310510580943415 1106 5 -0.32199027429437166 1106 6 -0.3854014032331321 1106 7 -0.3636859271107511 1106 8 -0.4620703504747681 1106 9 -0.5656488052482658 1106 10-11 -0.5484625492620268 1106 12-13 -0.612870392224707 1106 14-15 -0.7208480349219926 1106 16-17 -0.7111943155262992 1106 18-19 -0.7013246185449304 1106 20-21 -0.751107145947536 1106 22-23 -0.7022113369771503 1106 24-25 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2311 24-25 0.9177149057048268 2311 26-27 0.3884232463335522 2311 28-29 0.659817730442505 2311 30-31 0.8568062434556509 2311 32-33 0.9692470769675836 2311 34-35 0.980971438275752 2311 36-37 1.0371230921807424 2311 38-39 1.0793983099230715 2311 40-41 1.033539895332737 2311 42-43 0.9769007406314714 2311 44-45 1.0234190760161042 2311 46-47 0.6153590927974903 2311 48-49 0.8895776513344131 2311 50-51 1.0578493610785102 2311 52-53 0.9321945898661497 2311 54-55 0.9395320351376668 2311 56-57 1.0466460062145089 2311 58-59 1.1017944053244477 2311 60-61 1.121645275891801 2311 62-63 1.119749714236434 2311 64-65 1.1894135106117005 2311 66-67 1.1969396808553014 2311 68-69 1.2473446102963095 2311 70-71 1.1985753827500005 2311 72-73 1.2059952902992777 2311 74-75 1.248244069066594 2311 76-77 1.1886370888730475 2311 78-79 0.8936792285302744 2311 80-81 0.884840856963585 2311 82-83 0.9994920547277388 2311 84-85 1.083004450648243 2311 86-87 1.0524016898437871 2311 88-89 1.0439705371930472 2311 90-91 1.1128578324638418 2311 92-93 1.1965900850153481 2311 94-95 1.2127024481712994 2311 96-97 1.2209906014521188 2311 98-99 1.2926762002284669 2311 100 1.3299842957578285 2312 1 0.25807203505625154 2312 2 0.33555312172605767 2312 3 0.4430583513572586 2312 4 0.14283565800749187 2312 5 0.31143829227799813 2312 6 0.40664061821402697 2312 7 0.26275803770616335 2312 8 0.40151065644031547 2312 9 0.551644433000618 2312 10-11 0.5756273259836675 2312 12-13 0.646050949790073 2312 14-15 0.8233071903497731 2312 16-17 0.8237252835765858 2312 18-19 0.7643653456002824 2312 20-21 0.8149156321239488 2312 22-23 0.9154658448153725 2312 24-25 0.8932025346338648 2312 26-27 0.9058815318068412 2312 28-29 0.9103148034930726 2312 30-31 0.884798356788238 2312 32-33 0.7565440034877398 2312 34-35 0.8855515654568293 2312 36-37 0.9407085048726955 2312 38-39 0.7748446625170189 2312 40-41 0.9071936772833311 2312 42-43 0.9442084849958547 2312 44-45 0.7255421756375355 2312 46-47 0.7717084874252151 2312 48-49 0.9336199754927534 2312 50-51 0.7175287277351501 2312 52-53 0.9290300187393896 2312 54-55 0.7810715409797453 2312 56-57 0.8430147601368319 2312 58-59 0.9515474727189925 2312 60-61 0.9913593120837518 2312 62-63 1.047093034004476 2312 64-65 1.0286409562484025 2312 66-67 1.024802386454553 2312 68-69 1.082312911653915 2312 70-71 0.9787760208649772 2312 72-73 1.0027071374159746 2312 74-75 1.0146674798875566 2312 76-77 1.0188208778368413 2312 78-79 0.9479481668447605 2312 80-81 0.8067608672968802 2312 82-83 0.8671158085992658 2312 84-85 0.9110658078464873 2312 86-87 0.9424679944331054 2312 88-89 0.9094930672755055 2312 90-91 0.963676520748006 2312 92-93 1.0665460058170204 2312 94-95 1.0895819384807446 2312 96-97 0.7379193269493562 2312 98-99 0.5709230401866812 2312 100 0.6980307171409272 2313 1 0.022890477811252197 2313 2 0.19042838829993514 2313 3 0.24840889778056408 2313 4 0.15928407543029977 2313 5 0.30920657756897896 2313 6 0.22230132576748218 2313 7 0.24388010114153502 2313 8 0.37759663496133555 2313 9 0.5735262863233288 2313 10-11 0.3601624770036196 2313 12-13 0.40763551595378544 2313 14-15 0.4562586539431308 2313 16-17 0.7375466845103134 2313 18-19 0.6753104179165632 2313 20-21 0.6483185616728449 2313 22-23 0.5739084799353762 2313 24-25 0.4989475619739281 2313 26-27 0.7140533233770086 2313 28-29 0.5444516911290478 2313 30-31 0.7504567110033875 2313 32-33 0.8523900690604265 2313 34-35 0.9128871400422369 2313 36-37 0.9475397705753963 2313 38-39 0.9203498276959792 2313 40-41 0.8943226441199812 2313 42-43 0.9710939430304819 2313 44-45 0.8181814412967796 2313 46-47 0.8237834792520147 2313 48-49 0.9811975370350581 2313 50-51 0.8700505467540509 2313 52-53 0.8816863294183221 2313 54-55 0.9050332374903931 2313 56-57 0.6333578437907121 2313 58-59 0.7243512027690571 2313 60-61 0.6173350616559645 2313 62-63 0.9458223760051823 2313 64-65 1.0127155734308388 2313 66-67 0.9281906591644784 2313 68-69 1.0456655287787058 2313 70-71 0.9753159491912058 2313 72-73 0.9721374943108074 2313 74-75 1.0533059286992312 2313 76-77 1.0290518315642423 2313 78-79 0.9176692215513427 2313 80-81 0.8191204996729553 2313 82-83 0.862819829388485 2313 84-85 0.8463550723954931 2313 86-87 0.858896669798316 2313 88-89 0.8821565625752186 2313 90-91 0.775650976308949 2313 92-93 0.9778654272300535 2313 94-95 1.0492694305083319 2313 96-97 1.130022825764005 2313 98-99 1.1757371111064323 2313 100 1.229670036658245 2314 1 -0.45787116293461594 2314 2 -0.004948900805260337 2314 3 0.23528901442187333 2314 4 0.1885853154377486 2314 5 0.2575112767400398 2314 6 0.3624574913689784 2314 7 0.38577192409874783 2314 8 0.3835231293392738 2314 9 0.3842032213502975 2314 10-11 0.499818971945551 2314 12-13 0.38496421536511605 2314 14-15 0.6925806371250971 2314 16-17 0.7311616297111883 2314 18-19 0.7533209999125887 2314 20-21 0.7831468174546998 2314 22-23 0.8378806162211063 2314 24-25 0.7433859155881279 2314 26-27 0.6581270783257196 2314 28-29 0.70490189939418 2314 30-31 0.7330624661960314 2314 32-33 0.7557107675510295 2314 34-35 0.8170922889871122 2314 36-37 0.7751502549499989 2314 38-39 0.5597636590314821 2314 40-41 0.6160199573301952 2314 42-43 0.7575792007757798 2314 44-45 0.7894298375819346 2314 46-47 0.696587728259523 2314 48-49 0.6995104989484915 2314 50-51 0.6953665238577216 2314 52-53 0.68514071571024 2314 54-55 0.6465866238850282 2314 56-57 0.6376532403840045 2314 58-59 0.7404058613600597 2314 60-61 0.8135463197341508 2314 62-63 0.7181612840530605 2314 64-65 0.7725839650710711 2314 66-67 0.5283109119851304 2314 68-69 0.5269408397320205 2314 70-71 0.6706349904333919 2314 72-73 0.778792460966379 2314 74-75 0.8505477684387088 2314 76-77 0.6291658368896371 2314 78-79 0.618032599376015 2314 80-81 0.6719590577464558 2314 82-83 0.6780553439115593 2314 84-85 0.7020568031007066 2314 86-87 0.7530639852874295 2314 88-89 0.756142104037032 2314 90-91 0.8622474079459899 2314 92-93 0.7436640818061484 2314 94-95 0.751848390824243 2314 96-97 0.7410409743365527 2314 98-99 0.8732555859243298 2314 100 0.9254398862689968 2315 1 0.41598185221502604 2315 2 0.4260716975715084 2315 3 0.4162530955407888 2315 4 0.2763799478226261 2315 5 0.3339305941805719 2315 6 0.3968032793079672 2315 7 0.3509618626606823 2315 8 0.2625760300137827 2315 9 0.4497211014638012 2315 10-11 0.5357393653079043 2315 12-13 0.3905966956889557 2315 14-15 0.6156533902565329 2315 16-17 0.7224910978336254 2315 18-19 0.7368823975515255 2315 20-21 0.42105461743561534 2315 22-23 0.7865376375611604 2315 24-25 0.8467272562619783 2315 26-27 0.8597354582315759 2315 28-29 0.779526842755935 2315 30-31 0.8070227431534889 2315 32-33 0.7776408502402532 2315 34-35 0.8038967470177809 2315 36-37 0.8264077039115634 2315 38-39 0.8161264339009762 2315 40-41 0.8345574382950502 2315 42-43 0.9128607389877317 2315 44-45 0.953558960335819 2315 46-47 0.8922526061033267 2315 48-49 0.7825472585994007 2315 50-51 0.8619782320744847 2315 52-53 0.9000942086982562 2315 54-55 0.7250234343335293 2315 56-57 0.7300231602335501 2315 58-59 0.7262489024840022 2315 60-61 0.8676509756245494 2315 62-63 0.8834559949832439 2315 64-65 0.9705088910932957 2315 66-67 0.9768315864875774 2315 68-69 0.9887060260392602 2315 70-71 0.9291197542815226 2315 72-73 1.0518623766020099 2315 74-75 0.38972320872796473 2315 76-77 0.4293389092425919 2315 78-79 0.7039643166638285 2315 80-81 0.7205593301013877 2315 82-83 0.702701600598516 2315 84-85 0.743777134639334 2315 86-87 0.8059277137193028 2315 88-89 0.7765632234037128 2315 90-91 0.7958407374168353 2315 92-93 0.7873005373043149 2315 94-95 0.8795009421917577 2315 96-97 0.9697526598607915 2315 98-99 1.0080216573366805 2315 100 0.9658389582282609 2316 1 0.22450791161650585 2316 2 0.006567987010328125 2316 3 0.15152826364314365 2316 4 -0.02295856767602089 2316 5 0.24440469067743464 2316 6 0.31921725532370004 2316 7 0.38892307196233844 2316 8 0.40007769282913586 2316 9 0.5608064970392945 2316 10-11 0.5592500131949407 2316 12-13 0.38081068873914603 2316 14-15 0.509512161705338 2316 16-17 0.7747438853441793 2316 18-19 0.6882646861753443 2316 20-21 0.6807713090620737 2316 22-23 0.6947475400855723 2316 24-25 0.41133645477595593 2316 26-27 0.6770401105331274 2316 28-29 0.7977399189252239 2316 30-31 0.45609506147624046 2316 32-33 0.5963792669631545 2316 34-35 0.7354447897411518 2316 36-37 0.7770325839029439 2316 38-39 0.7436937079491983 2316 40-41 0.7613086725404372 2316 42-43 0.6978422170304412 2316 44-45 0.48534525590824984 2316 46-47 0.6566089099642483 2316 48-49 0.7229196028349847 2316 50-51 0.781209139342657 2316 52-53 0.7625680174753953 2316 54-55 0.6604795445855629 2316 56-57 0.8274987695161755 2316 58-59 0.6799448933383658 2316 60-61 0.7376267338833955 2316 62-63 0.7704036618952941 2316 64-65 0.7981689141229182 2316 66-67 0.7888845261645443 2316 68-69 0.8355223591296124 2316 70-71 0.7717944356680491 2316 72-73 0.7856333927823371 2316 74-75 0.80567188025136 2316 76-77 0.6791272764873248 2316 78-79 0.41697113300293864 2316 80-81 0.4422359791778163 2316 82-83 0.3767396242950696 2316 84-85 0.5082486129167982 2316 86-87 0.5094900962312359 2316 88-89 0.4982026284258083 2316 90-91 0.4511971246442421 2316 92-93 0.27453423479170525 2316 94-95 0.32474635116608397 2316 96-97 0.554864108367088 2316 98-99 0.6541134731852232 2316 100 0.32709612789682296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 12.0 12 32.0 13 181.0 14 744.0 15 2157.0 16 5396.0 17 11730.0 18 20380.0 19 30444.0 20 43783.0 21 61337.0 22 75864.0 23 74744.0 24 70441.0 25 71884.0 26 81946.0 27 98819.0 28 122310.0 29 150723.0 30 185015.0 31 232494.0 32 296865.0 33 392416.0 34 575561.0 35 807080.0 36 1282184.0 37 1904364.0 38 1589298.0 39 287381.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3176768721812 21.33239407393103 13.571570468711766 22.778358585175997 2 15.265289700836751 20.37231406713829 41.445896711044824 22.91649952098013 3 18.83865997140039 27.027917655775845 29.248623112826877 24.884799259996885 4 12.09372109810557 16.883993962676044 39.825565338168985 31.196719601049406 5 12.56162366499757 38.828501446498436 34.701733438418806 13.908141450085184 6 29.221368533821636 38.45618737936014 18.17944131652595 14.143002770292274 7 26.813458725853543 32.50147600657513 23.09874778952972 17.5863174780416 8 26.169775969720284 35.46877875889018 19.810185425525386 18.551259845864145 9 26.354152617481862 15.539740622991447 21.944080321040612 36.16202643848608 10-11 25.701399823458022 26.519447668894884 27.121976963413054 20.657175544234043 12-13 25.785895161278425 24.334895162753245 27.92486700852886 21.95434266743947 14-15 23.047006074358148 25.451807228915662 26.13232907343267 25.36885762329352 16-17 21.259792352629347 28.103227622666854 27.38966322105795 23.247316803645845 18-19 20.66865747011435 27.93052206087092 30.037672671326295 21.363147797688434 20-21 22.33716537478767 26.96558336191525 29.515231830897942 21.182019432399137 22-23 22.47924050573172 26.834893884119666 29.14548045929275 21.540385150855865 24-25 21.92742908439777 26.681383840679796 30.087821071476416 21.303366003446015 26-27 21.78980460357332 27.011907135668434 29.204645340324394 21.99364292043386 28-29 21.011998988629873 27.548527065418494 29.700832893739708 21.738641052211925 30-31 22.01875335491293 27.00200880343312 29.62354713355118 21.355690708102767 32-33 22.266791340884666 27.286094879455696 28.802393527859888 21.644720251799754 34-35 22.257621250227107 27.118865498622657 29.53388290044022 21.089630350710014 36-37 21.91366093221682 26.75430485015655 29.22044914028101 22.111585077345616 38-39 21.962714568074055 27.028837389777845 29.683869758312696 21.324578283835404 40-41 21.69453737696425 26.188867498563067 30.21397647546594 21.90261864900674 42-43 21.743354051352032 26.375060932023548 29.378559136612033 22.50302588001239 44-45 21.788591120763353 27.191232979516126 29.238623363735535 21.781552535984986 46-47 21.87246194671729 27.101589984821462 28.576459846208536 22.449488222252707 48-49 22.54397286159618 26.977030322984742 28.61162572322068 21.867371092198397 50-51 22.089254391127316 26.862396761478518 28.570055582291896 22.478293265102263 52-53 21.71035902310712 27.511092661527243 28.12016099123667 22.658387324128967 54-55 21.70705828518458 27.30886283982896 28.166318928944218 22.817759946042244 56-57 22.596090457135485 26.854399417055642 27.810108187691142 22.739401938117734 58-59 21.65164676707701 27.33580427009164 28.509213092011983 22.503335870819367 60-61 21.797385368353904 27.998146134991742 27.85553705007388 22.34893144658047 62-63 22.076916356219076 27.277245090765106 27.603649399450642 23.042189153565175 64-65 21.314399370399624 27.45677398734473 27.86187622422951 23.366950418026136 66-67 21.806013733746788 27.076157405480807 27.777495428449562 23.340333432322844 68-69 21.442025854067207 27.149208393986324 27.987313148403615 23.421452603542853 70-71 21.47021574986975 26.75518657700644 28.184551523851464 23.590046149272347 72-73 21.95904763984492 26.886651530165683 28.2876415210708 22.866659308918592 74-75 22.11944045925864 27.370819344037795 27.690752833940685 22.81898736276288 76-77 21.838928455724975 27.182168545870088 27.46261214291742 23.516290855487515 78-79 21.725836415708766 27.22361298120649 27.534838940315403 23.515711662769338 80-81 21.31769194365862 27.71845286209846 27.867487490777883 23.09636770346504 82-83 22.217120718828845 26.734333453566194 28.839368503648533 22.20917732395643 84-85 22.347594076846825 27.18235295332818 27.637735857965673 22.83231711185932 86-87 21.097488784044447 27.912502118292288 27.971693299254923 23.01831579840834 88-89 21.942667695426486 26.872113623255483 27.81224818456754 23.372970496750494 90-91 21.76423229149397 26.824503576461584 27.945954301394966 23.465309830649485 92-93 22.103792184762426 27.064551698154897 27.750912023220884 23.080744093861792 94-95 21.011944116436165 26.98590214063309 28.378802222985556 23.62335151994519 96-97 21.22220108899569 27.053370888597637 27.975405565233313 23.74902245717336 98-99 20.65412065566071 27.591515791281125 27.74144954251324 24.01291401054493 100 21.22741791360624 26.985648564567295 27.144179186812973 24.642754335013493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1598.0 1 1093.5 2 1126.5 3 1568.0 4 1168.5 5 978.0 6 1516.0 7 2743.0 8 4295.0 9 5194.5 10 4862.5 11 4446.5 12 4951.0 13 5859.5 14 6919.0 15 8302.0 16 9759.5 17 11173.5 18 12632.5 19 13931.5 20 15274.0 21 16763.5 22 18637.5 23 20972.5 24 23545.0 25 27049.5 26 32510.5 27 38787.5 28 46018.0 29 60125.5 30 82041.5 31 103369.5 32 132606.0 33 166207.0 34 193812.5 35 221858.5 36 247386.5 37 282986.5 38 296724.5 39 282611.0 40 263286.0 41 243503.5 42 238550.0 43 245665.5 44 263614.0 45 303046.0 46 338565.0 47 363500.5 48 389188.0 49 384009.0 50 359064.0 51 356119.5 52 351073.0 53 325451.0 54 302729.0 55 262975.5 56 226953.0 57 185745.0 58 144430.5 59 137795.5 60 113912.0 61 76188.0 62 55780.0 63 46597.5 64 33678.0 65 16030.0 66 8747.5 67 6103.0 68 4667.5 69 3643.0 70 2855.0 71 2216.0 72 1810.0 73 1668.0 74 1322.5 75 908.0 76 602.5 77 364.5 78 224.5 79 154.0 80 101.0 81 62.5 82 49.5 83 31.5 84 18.0 85 13.5 86 8.5 87 3.5 88 2.5 89 2.5 90 1.0 91 1.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043855095308436884 2 0.0 3 0.0 4 0.03553713937826524 5 0.0 6 0.0 7 8.022992953924423E-4 8 0.0 9 0.011196794578344526 10-11 4.1884742627105445E-4 12-13 2.9496297624722143E-5 14-15 1.415822285986663E-4 16-17 2.47768900047666E-4 18-19 0.0 20-21 1.179851904988886E-5 22-23 0.0 24-25 6.430192882189428E-4 26-27 5.899259524944428E-4 28-29 1.0618667144899973E-4 30-31 0.0023184089933031607 32-33 0.015184694017206962 34-35 0.026469977488425656 36-37 0.03182060587755025 38-39 0.022434883973363663 40-41 0.0012447437597632746 42-43 7.669037382427758E-5 44-45 0.010913630121147194 46-47 0.01772727487245801 48-49 0.0031914994029949365 50-51 0.010718954556824027 52-53 0.01053607751155075 54-55 0.010170323421004195 56-57 5.368326167699431E-4 58-59 0.008453638899245367 60-61 0.004406746865133489 62-63 0.0012801393169129412 64-65 0.03497081046387057 66-67 0.05952942786621423 68-69 0.058762524127971456 70-71 0.051034494150294256 72-73 0.045365305746822654 74-75 0.034705343785248075 76-77 0.031089097696457138 78-79 0.014754048071886017 80-81 0.0010972622716396638 82-83 0.037660872807245234 84-85 0.05082212080739626 86-87 0.056438215875143354 88-89 0.06955226979909482 90-91 0.04800227475447282 92-93 0.03855756025503679 94-95 0.012872184283428744 96-97 0.003633943867365768 98-99 0.02238768989716411 100 0.03337801039213558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8475640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.26406615700488 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64905535715572 47.858668428300234 2 15.217558192605141 19.254492164900864 3 4.84193674945562 9.189618205367804 4 1.9148198224308621 4.845571517000416 5 0.889200554111152 2.8127221341066653 6 0.47079244914221785 1.7870546789250974 7 0.264379303072666 1.1707996804092387 8 0.1638579863364453 0.829305799035398 9 0.12466300823626537 0.7098019920351302 >10 0.4119962824637738 4.450758985413122 >50 0.026786196325330774 1.1876020946744452 >100 0.0218764354742029 2.7574882527577906 >500 0.002035659396859693 0.8665695033427708 >1k 9.208405628142417E-4 0.9033224337536087 >5k 4.846529236623592E-5 0.21879771396943284 >10k+ 7.269793854935389E-5 1.1574264160078647 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31929 0.37671491474390134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27102 0.3197634632900878 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16467 0.19428621319451983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.5395557149666574E-5 0.0 9 0.0 0.0 0.0 3.5395557149666574E-5 0.0 10-11 0.0 0.0 0.0 4.1294816674611E-5 0.0 12-13 0.0 1.1798519049888857E-5 0.0 8.848889287416643E-5 0.0 14-15 0.0 1.1798519049888857E-5 0.0 9.438815239911086E-5 0.0 16-17 0.0 1.1798519049888857E-5 0.0 1.1208593097394414E-4 0.0 18-19 0.0 1.1798519049888857E-5 0.0 1.65179266698444E-4 1.1798519049888857E-5 20-21 0.0 1.1798519049888857E-5 0.0 2.3007112147283273E-4 1.1798519049888857E-5 22-23 0.0 1.1798519049888857E-5 0.0 5.073363191452209E-4 1.1798519049888857E-5 24-25 5.899259524944429E-6 2.3597038099777715E-5 0.0 0.001073665233539886 1.1798519049888857E-5 26-27 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.0020293452765808837 1.1798519049888857E-5 28-29 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.005574800251072485 1.1798519049888857E-5 30-31 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.019278780127518394 1.1798519049888857E-5 32-33 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.05192528233856086 1.1798519049888857E-5 34-35 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.10136697641712011 1.1798519049888857E-5 36-37 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.1833017919590733 1.1798519049888857E-5 38-39 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.30167633358660817 1.1798519049888857E-5 40-41 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.42669344143923055 1.1798519049888857E-5 42-43 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.548931998055604 1.1798519049888857E-5 44-45 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.6923194000689034 1.1798519049888857E-5 46-47 1.1798519049888857E-5 2.3597038099777715E-5 0.0 0.8607491587655918 1.1798519049888857E-5 48-49 1.1798519049888857E-5 2.3597038099777715E-5 0.0 1.0469120915942631 1.1798519049888857E-5 50-51 1.1798519049888857E-5 2.3597038099777715E-5 0.0 1.2360246541854067 1.1798519049888857E-5 52-53 1.1798519049888857E-5 2.3597038099777715E-5 0.0 1.4173265971655238 1.7697778574833287E-5 54-55 1.1798519049888857E-5 2.3597038099777715E-5 0.0 1.6132528045079781 3.5395557149666574E-5 56-57 1.1798519049888857E-5 2.3597038099777715E-5 0.0 1.8429050785545398 4.719407619955543E-5 58-59 1.1798519049888857E-5 2.3597038099777715E-5 0.0 2.1037998310452073 4.719407619955543E-5 60-61 1.1798519049888857E-5 2.3597038099777715E-5 0.0 2.3675616236649977 5.3093335724499865E-5 62-63 1.1798519049888857E-5 2.3597038099777715E-5 0.0 2.6306096058822694 5.899259524944429E-5 64-65 1.1798519049888857E-5 2.3597038099777715E-5 0.0 2.8999461987531325 5.899259524944429E-5 66-67 1.1798519049888857E-5 2.3597038099777715E-5 0.0 3.2009205204562723 5.899259524944429E-5 68-69 1.1798519049888857E-5 2.3597038099777715E-5 0.0 3.5306124375268415 5.899259524944429E-5 70-71 1.1798519049888857E-5 2.3597038099777715E-5 0.0 3.8842553482686855 5.899259524944429E-5 72-73 1.1798519049888857E-5 2.3597038099777715E-5 0.0 4.23775667678193 5.899259524944429E-5 74-75 1.1798519049888857E-5 2.3597038099777715E-5 0.0 4.57743604022823 5.899259524944429E-5 76-77 1.1798519049888857E-5 2.3597038099777715E-5 0.0 4.956528356560685 5.899259524944429E-5 78-79 1.1798519049888857E-5 2.3597038099777715E-5 0.0 5.36992486703069 5.899259524944429E-5 80-81 1.1798519049888857E-5 2.3597038099777715E-5 0.0 5.804004181395151 6.489185477438872E-5 82-83 1.1798519049888857E-5 2.3597038099777715E-5 0.0 6.246696414666031 8.2589633349222E-5 84-85 1.7697778574833287E-5 2.3597038099777715E-5 0.0 6.67033403967134 8.2589633349222E-5 86-87 2.3597038099777715E-5 2.3597038099777715E-5 0.0 7.115781227140369 8.2589633349222E-5 88 2.3597038099777715E-5 2.3597038099777715E-5 0.0 7.472745420994757 8.2589633349222E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4810 0.0 37.715828 2 GTACTAG 8415 0.0 34.80823 1 GGTATCA 26825 0.0 33.93233 1 GGGCACC 8840 0.0 33.611843 94 ACCTGGG 5415 0.0 32.551662 3 GTATCAA 40605 0.0 31.089434 1 TAGGCAT 10200 0.0 31.048906 5 CTGTGCG 10395 0.0 30.963688 9 TAGTACT 11305 0.0 29.146595 4 GCATCGT 9645 0.0 28.450811 8 ACTGTGC 11950 0.0 28.231932 8 CTAGGCA 11745 0.0 27.49439 4 CCTGGGG 4945 0.0 27.28073 4 GTACTGT 12365 0.0 26.790546 6 CAACGCA 45110 0.0 26.738659 5 ATCAACG 45605 0.0 26.46811 3 GTACCTG 7030 0.0 26.417385 1 GTACATG 42075 0.0 26.360392 1 GAGTTGC 9320 0.0 26.231007 94 TACATGG 42280 0.0 25.866806 2 >>END_MODULE