FastQCFastQC Report
Mon 6 Jun 2016
ERR1211201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269070.25969957198360205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217870.21028262440282225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128160.12369679691313948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169450.045.3559381
GTATCAA297400.039.7686731
GTACTAG84000.037.8753781
CAACGCA333200.033.7473755
ATCAACG339400.033.1726073
GCATCGT85700.032.6895878
TATCAAC372700.031.2912622
AACGCAG382450.030.0778886
GGGCACC101050.029.1608294
CTGTGCG115350.028.9328389
TCAACGC397300.028.6691284
TAGGCAT111550.028.572225
TAGTACT121600.028.4140664
CTAACGC40100.028.1353823
TCTAACG41250.028.0667632
CTAGGCA120550.027.4137044
TAACGCC41000.027.4027214
GTGGTAT62400.027.30321
ACGCAGA410750.027.284947
GTACATG422300.026.992471
Produced by FastQC (version 0.11.5)