##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10360818 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496933253725718 31.0 31.0 34.0 30.0 34.0 2 31.5137628129362 31.0 31.0 34.0 28.0 34.0 3 31.580888304379055 33.0 31.0 34.0 28.0 34.0 4 35.176773590656644 37.0 35.0 37.0 32.0 37.0 5 35.23337278967742 37.0 35.0 37.0 32.0 37.0 6 35.29413642822411 37.0 35.0 37.0 32.0 37.0 7 35.209513573156094 37.0 35.0 37.0 32.0 37.0 8 35.213605045470345 37.0 35.0 37.0 32.0 37.0 9 36.78505934570031 39.0 37.0 39.0 32.0 39.0 10-11 36.74194836739724 39.0 37.0 39.0 32.0 39.0 12-13 36.637250118668234 39.0 37.0 39.0 32.0 39.0 14-15 37.84579335338194 40.0 38.0 41.0 32.0 41.0 16-17 37.82409217110077 40.0 37.5 41.0 32.0 41.0 18-19 37.833470725960055 40.0 37.5 41.0 32.0 41.0 20-21 37.79771331761643 40.0 37.5 41.0 32.0 41.0 22-23 37.690568157842364 40.0 37.0 41.0 32.0 41.0 24-25 37.59663348974955 40.0 37.0 41.0 32.0 41.0 26-27 37.45163210086308 40.0 37.0 41.0 31.0 41.0 28-29 37.34187778416724 40.0 37.0 41.0 31.0 41.0 30-31 37.19698097196573 40.0 36.0 41.0 31.0 41.0 32-33 37.02934005789891 39.0 36.0 41.0 30.5 41.0 34-35 36.89511619642387 39.0 36.0 41.0 30.0 41.0 36-37 36.798284121967974 39.0 35.5 41.0 30.0 41.0 38-39 36.66006366485735 39.0 35.0 41.0 30.0 41.0 40-41 36.49359201175042 39.0 35.0 41.0 29.5 41.0 42-43 36.28339437098499 39.0 35.0 40.0 29.0 41.0 44-45 36.06189564376095 38.5 35.0 40.0 28.0 41.0 46-47 35.83266297120555 38.0 35.0 40.0 27.5 41.0 48-49 35.62753124318949 38.0 34.0 40.0 27.0 41.0 50-51 34.93519039712888 37.5 33.5 39.5 26.0 40.5 52-53 35.02592536612457 38.0 33.5 40.0 26.5 40.5 54-55 35.50133087947303 38.0 34.0 40.0 27.0 41.0 56-57 35.45050916829154 38.0 34.0 40.0 27.0 41.0 58-59 35.22166970793233 38.0 34.0 40.0 26.5 41.0 60-61 34.997021808509714 37.0 34.0 40.0 26.0 41.0 62-63 34.69625863517726 37.0 34.0 40.0 26.0 41.0 64-65 34.44073489178171 36.0 33.0 39.5 26.0 41.0 66-67 34.271525761769006 36.0 33.0 39.0 26.0 41.0 68-69 33.980522339066276 35.0 33.0 39.0 26.0 41.0 70-71 33.69630235759377 35.0 33.0 38.5 25.0 41.0 72-73 33.377072495627274 35.0 33.0 37.5 25.0 40.0 74-75 33.05656160546397 35.0 32.0 37.0 24.5 39.5 76-77 32.74239644977838 35.0 32.0 36.5 24.0 39.0 78-79 32.42106347201543 35.0 32.0 36.0 24.0 39.0 80-81 32.136245806074385 35.0 31.5 36.0 23.0 38.0 82-83 31.85158131336734 35.0 31.0 35.0 23.0 37.0 84-85 31.615362898952576 35.0 31.0 35.0 22.0 37.0 86-87 31.40029035352228 34.5 31.0 35.0 20.5 36.0 88-89 31.19805583883435 34.0 31.0 35.0 20.0 36.0 90-91 30.97800028916636 34.0 30.5 35.0 20.0 36.0 92-93 30.783329656017507 34.0 30.0 35.0 19.5 35.5 94-95 30.619401962277497 34.0 30.0 35.0 19.0 35.0 96-97 30.443548955304493 34.0 30.0 35.0 18.5 35.0 98-99 30.214729184510336 34.0 29.0 35.0 18.0 35.0 100 29.06014476849222 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43879508122380173 1101 2 -0.3962767177019231 1101 3 -0.4074504584411578 1101 4 -0.3070332431465488 1101 5 -0.24081495555616783 1101 6 -0.2834010425608753 1101 7 -0.2749127500299977 1101 8 -0.31872662891179004 1101 9 -0.5128545940809985 1101 10-11 -0.43767664295712905 1101 12-13 -0.4702828887362571 1101 14-15 -0.6020534392911117 1101 16-17 -0.66078931701486 1101 18-19 -0.6082720912384971 1101 20-21 -0.6584327509478598 1101 22-23 -0.7428044911453284 1101 24-25 -0.7663126420483408 1101 26-27 -0.8108793348696182 1101 28-29 -0.7412425613007798 1101 30-31 -0.8398845945456301 1101 32-33 -0.8184916049122535 1101 34-35 -0.8358847522099282 1101 36-37 -0.9831031180226475 1101 38-39 -0.983997674344657 1101 40-41 -0.9551951196836725 1101 42-43 -1.031427925924639 1101 44-45 -1.0558234773485324 1101 46-47 -1.2147427364325054 1101 48-49 -1.1253122447598258 1101 50-51 -1.1639491115996208 1101 52-53 -1.0194639782636088 1101 54-55 -0.9856007651532295 1101 56-57 -0.8799971437232941 1101 58-59 -0.8469271073264224 1101 60-61 -0.8033104375515521 1101 62-63 -0.8329174112328985 1101 64-65 -0.8181047412849907 1101 66-67 -0.8761682203764067 1101 68-69 -0.8757597837574664 1101 70-71 -0.9183284600650481 1101 72-73 -0.9947910774316711 1101 74-75 -1.0135353748176144 1101 76-77 -0.9612016750883612 1101 78-79 -0.9829303979938082 1101 80-81 -1.0628124970713664 1101 82-83 -1.089804835365868 1101 84-85 -1.2137884454175598 1101 86-87 -1.2643136160235606 1101 88-89 -1.3733571210327504 1101 90-91 -1.4141321579007595 1101 92-93 -1.5273496296812503 1101 94-95 -1.6294246312584697 1101 96-97 -1.6274485177819535 1101 98-99 -1.6295583378121776 1101 100 -1.7089730446993059 1102 1 -0.4736397066689868 1102 2 -0.36906227929799584 1102 3 -0.3973233064981869 1102 4 -0.30122644432594825 1102 5 -0.3416016830317403 1102 6 -0.30314654616781667 1102 7 -0.30781233773327443 1102 8 -0.38197730962213683 1102 9 -0.507097507911034 1102 10-11 -0.5442158091195992 1102 12-13 -0.5606123498021134 1102 14-15 -0.6947165040262462 1102 16-17 -0.7239387442670875 1102 18-19 -0.7434549014717291 1102 20-21 -0.754974520251487 1102 22-23 -0.7349322219972976 1102 24-25 -0.7225936972432976 1102 26-27 -0.7713332681969831 1102 28-29 -0.8672960353070351 1102 30-31 -0.841291234589697 1102 32-33 -0.9182056393320579 1102 34-35 -1.006829724391494 1102 36-37 -0.9991717136392779 1102 38-39 -1.0170256955729045 1102 40-41 -1.1058424353321428 1102 42-43 -1.0511987263766187 1102 44-45 -1.2368756239036713 1102 46-47 -1.15732001050241 1102 48-49 -1.136006152857739 1102 50-51 -1.1319149854618402 1102 52-53 -1.0399657991683995 1102 54-55 -0.9178236561945212 1102 56-57 -0.9449247453532124 1102 58-59 -0.915134262650831 1102 60-61 -0.8984660785106655 1102 62-63 -1.0403143937410775 1102 64-65 -1.002614180229493 1102 66-67 -0.9692455663776869 1102 68-69 -1.0834743589592861 1102 70-71 -1.1591397665587735 1102 72-73 -1.0771662831227218 1102 74-75 -1.1305335309231985 1102 76-77 -1.1509976700746734 1102 78-79 -1.1400164269912167 1102 80-81 -1.193759346747985 1102 82-83 -1.2207772682253477 1102 84-85 -1.2535935597127725 1102 86-87 -1.3038093973699212 1102 88-89 -1.312061610017924 1102 90-91 -1.3151076359629492 1102 92-93 -1.3807483224852284 1102 94-95 -1.4669828956358728 1102 96-97 -1.4829253623748642 1102 98-99 -1.5711502298352436 1102 100 -1.623946802333748 1103 1 -0.5690429042042631 1103 2 -0.5186266925820924 1103 3 -0.5969347391692743 1103 4 -0.5118079174117582 1103 5 -0.43799619946732804 1103 6 -0.43633782260488374 1103 7 -0.44990799736567766 1103 8 -0.5348944902831079 1103 9 -0.6346746951424009 1103 10-11 -0.6541710046685623 1103 12-13 -0.6869149562668966 1103 14-15 -0.8340540704640134 1103 16-17 -0.8217658040023963 1103 18-19 -0.7740156371680555 1103 20-21 -0.8201667256642438 1103 22-23 -0.8826704141799695 1103 24-25 -0.8788205517699694 1103 26-27 -0.9231424005109545 1103 28-29 -0.9990670808761379 1103 30-31 -0.9565998266536937 1103 32-33 -0.951863792333647 1103 34-35 -0.9601238813387951 1103 36-37 -1.0185155039841831 1103 38-39 -0.9804217146241783 1103 40-41 -1.043830362900593 1103 42-43 -1.182764213999036 1103 44-45 -1.1952496081497443 1103 46-47 -1.1771139368438313 1103 48-49 -1.1939556652051664 1103 50-51 -1.193659796764436 1103 52-53 -1.1572656802791244 1103 54-55 -0.8908383367787991 1103 56-57 -0.8453376045133538 1103 58-59 -0.873761866719299 1103 60-61 -0.978009958129924 1103 62-63 -0.8821034749073675 1103 64-65 -0.8746336116984494 1103 66-67 -0.9378558514650379 1103 68-69 -0.9086496684612797 1103 70-71 -0.9073902893326888 1103 72-73 -1.003980185283595 1103 74-75 -0.9497045610361283 1103 76-77 -0.9290077309477702 1103 78-79 -0.983369713117419 1103 80-81 -1.0913252516696303 1103 82-83 -1.06234557176057 1103 84-85 -1.085768551760136 1103 86-87 -1.1978201066037428 1103 88-89 -1.1251537918819352 1103 90-91 -1.2407769484319857 1103 92-93 -1.3729791454985012 1103 94-95 -1.369799016724766 1103 96-97 -1.4089815564002173 1103 98-99 -1.4193820377128823 1103 100 -1.4737396475677187 1104 1 -0.49898738526815833 1104 2 -0.53198755173214 1104 3 -0.5819314729769189 1104 4 -0.3950882850832471 1104 5 -0.3711892012437801 1104 6 -0.416243434501844 1104 7 -0.4493636601240283 1104 8 -0.41820027809419713 1104 9 -0.5213216478292679 1104 10-11 -0.5425630900532141 1104 12-13 -0.5669041125927308 1104 14-15 -0.7564210697178808 1104 16-17 -0.8607185892600739 1104 18-19 -0.8723390480609652 1104 20-21 -0.8349868887331766 1104 22-23 -0.7971525252845595 1104 24-25 -0.8355590739646672 1104 26-27 -0.8841720491767546 1104 28-29 -0.9244635843823872 1104 30-31 -0.9087974019439642 1104 32-33 -0.9530119845690166 1104 34-35 -1.1493162577460296 1104 36-37 -1.099876682786153 1104 38-39 -1.0640433540036085 1104 40-41 -1.0642299299137008 1104 42-43 -1.146614041357445 1104 44-45 -1.1336732153520117 1104 46-47 -1.1830566862134972 1104 48-49 -1.146337694855518 1104 50-51 -1.206760194492503 1104 52-53 -0.9659629677982196 1104 54-55 -0.9994297211249759 1104 56-57 -1.0181252711852196 1104 58-59 -0.9605716856493629 1104 60-61 -1.0158518579517803 1104 62-63 -0.9882901752761271 1104 64-65 -0.9193879632083153 1104 66-67 -0.9831217516145614 1104 68-69 -1.0043282130611644 1104 70-71 -1.026979811083585 1104 72-73 -0.9693507450460501 1104 74-75 -1.0400258956694657 1104 76-77 -1.0407908207277359 1104 78-79 -1.1162123962342676 1104 80-81 -1.0910572634372144 1104 82-83 -1.1176377956891983 1104 84-85 -1.1362771867957555 1104 86-87 -1.1195724515271657 1104 88-89 -1.2099057512806546 1104 90-91 -1.2215840146396246 1104 92-93 -1.2868414401263664 1104 94-95 -1.239737336994036 1104 96-97 -1.4137578718396604 1104 98-99 -1.3870684904252997 1104 100 -1.3897355026492733 1105 1 -0.55753603831619 1105 2 -0.5537172993905664 1105 3 -0.5626446503216336 1105 4 -0.3430640280115256 1105 5 -0.30019638731148746 1105 6 -0.2881569270490587 1105 7 -0.3559784761203417 1105 8 -0.37969085634923516 1105 9 -0.5587139175513443 1105 10-11 -0.5533744090341273 1105 12-13 -0.5626288089832201 1105 14-15 -0.697119585498605 1105 16-17 -0.7206699145577176 1105 18-19 -0.7259364655975915 1105 20-21 -0.7665576830667149 1105 22-23 -0.7494326203100883 1105 24-25 -0.73726517087254 1105 26-27 -0.7936998475232073 1105 28-29 -0.7585721602353814 1105 30-31 -0.8120860604636775 1105 32-33 -0.833974820461961 1105 34-35 -0.8293983963062601 1105 36-37 -0.866999551551423 1105 38-39 -0.9304698512947027 1105 40-41 -0.8980555206807068 1105 42-43 -0.9078358418777768 1105 44-45 -0.9143718684528537 1105 46-47 -0.951020323844709 1105 48-49 -0.9027439955253271 1105 50-51 -0.8986385542141875 1105 52-53 -0.8265839927767473 1105 54-55 -0.7843091382116114 1105 56-57 -0.6999280837126207 1105 58-59 -0.7025493911990992 1105 60-61 -0.6890099461598851 1105 62-63 -0.7293218508034656 1105 64-65 -0.6978571184677236 1105 66-67 -0.6939563622010994 1105 68-69 -0.7392641954757408 1105 70-71 -0.7044170056123349 1105 72-73 -0.6753595039573383 1105 74-75 -0.766956801464687 1105 76-77 -0.6720363248140657 1105 78-79 -0.775959220178251 1105 80-81 -0.7453290812281956 1105 82-83 -0.868524144273163 1105 84-85 -0.7834228295754109 1105 86-87 -0.8772167459288376 1105 88-89 -0.8843127660151033 1105 90-91 -1.003093384625462 1105 92-93 -0.8706076663475386 1105 94-95 -0.9638598878104041 1105 96-97 -0.9890623263988481 1105 98-99 -0.9142432450712121 1105 100 -0.9939072880115845 1106 1 -0.5681669350146699 1106 2 -0.5307444943117652 1106 3 -0.4954928376483565 1106 4 -0.41465048228417345 1106 5 -0.45658863286305973 1106 6 -0.4362712819852561 1106 7 -0.41827347329662246 1106 8 -0.43181172012744184 1106 9 -0.5367149711811905 1106 10-11 -0.5429202092287042 1106 12-13 -0.6162780125271965 1106 14-15 -0.7923767911123463 1106 16-17 -0.8070010715603075 1106 18-19 -0.8684701274979219 1106 20-21 -0.7310667306739091 1106 22-23 -0.7580420950694631 1106 24-25 -0.7091251238762126 1106 26-27 -0.7455165885570878 1106 28-29 -0.7814073134993009 1106 30-31 -0.8573517733464158 1106 32-33 -0.8623753709183717 1106 34-35 -0.8988700210890386 1106 36-37 -0.9066822654381284 1106 38-39 -0.8816369042096994 1106 40-41 -0.9162162609758226 1106 42-43 -0.8464346090080213 1106 44-45 -0.8719292704288009 1106 46-47 -0.9005918678218947 1106 48-49 -0.9297006754771218 1106 50-51 -0.9460029279267133 1106 52-53 -0.881629864682381 1106 54-55 -0.6820430420438086 1106 56-57 -0.7181156388765189 1106 58-59 -0.722319637825855 1106 60-61 -0.6699835063867638 1106 62-63 -0.7398286997745984 1106 64-65 -0.8254452112651691 1106 66-67 -0.8000152618119856 1106 68-69 -0.7379254077922823 1106 70-71 -0.7437995729196984 1106 72-73 -0.772011912462709 1106 74-75 -0.7258065378794356 1106 76-77 -0.7290899391426535 1106 78-79 -0.7544509907558989 1106 80-81 -0.6937382542516097 1106 82-83 -0.7285953876673723 1106 84-85 -0.799551200008306 1106 86-87 -0.8129945479156042 1106 88-89 -0.8193692604233114 1106 90-91 -0.8589544188442915 1106 92-93 -0.8636073962053814 1106 94-95 -0.9968425589506325 1106 96-97 -0.9994803689640683 1106 98-99 -0.9930825367793759 1106 100 -1.0687982694336249 1107 1 -0.5955435000878389 1107 2 -0.614563311056834 1107 3 -0.599810213984135 1107 4 -0.5576557670284288 1107 5 -0.40951189438071367 1107 6 -0.4727520593256287 1107 7 -0.49794085959523215 1107 8 -0.45002890107949156 1107 9 -0.6518543698174 1107 10-11 -0.6276108140571708 1107 12-13 -0.7195005422802367 1107 14-15 -0.8456812747257416 1107 16-17 -0.8788447806811917 1107 18-19 -0.8905433436095862 1107 20-21 -0.8327201061048513 1107 22-23 -0.9465829727352641 1107 24-25 -0.8988149173979494 1107 26-27 -0.9127347275554385 1107 28-29 -0.9676648000490431 1107 30-31 -0.975057310821434 1107 32-33 -0.9811290612185246 1107 34-35 -0.9691077785948892 1107 36-37 -1.1066813330616228 1107 38-39 -1.0130051347147528 1107 40-41 -1.0182204390501894 1107 42-43 -1.0103843562483519 1107 44-45 -0.9966517224980649 1107 46-47 -0.9375047283425033 1107 48-49 -0.9176557038435291 1107 50-51 -0.9082960219670682 1107 52-53 -0.8228045346826534 1107 54-55 -0.7634834691052177 1107 56-57 -0.7959811192776769 1107 58-59 -0.7598009910987216 1107 60-61 -0.7848647405680893 1107 62-63 -0.7999190645059429 1107 64-65 -0.7278605138521712 1107 66-67 -0.7639374134911705 1107 68-69 -0.6817737075453962 1107 70-71 -0.759003122484522 1107 72-73 -0.664965654235175 1107 74-75 -0.6895495790697765 1107 76-77 -0.7261274974731862 1107 78-79 -0.7353087767066206 1107 80-81 -0.740431685369078 1107 82-83 -0.7529778199043129 1107 84-85 -0.728046850241789 1107 86-87 -0.8152024925383934 1107 88-89 -0.7238644584060197 1107 90-91 -0.8080874681999397 1107 92-93 -0.8491120781163062 1107 94-95 -0.8824354772910006 1107 96-97 -0.9365978042354648 1107 98-99 -0.8975024274294867 1107 100 -0.8680067727758036 1108 1 -0.5355305445799381 1108 2 -0.5224744928386471 1108 3 -0.5658231961206504 1108 4 -0.34881533624247396 1108 5 -0.3825754165207016 1108 6 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72-73 1.1837600358992546 2207 74-75 1.1165198993594743 2207 76-77 1.1379231576670392 2207 78-79 1.0838455439954018 2207 80-81 1.0672640755914813 2207 82-83 1.1580226648920977 2207 84-85 1.1535094693468686 2207 86-87 1.1620294458944436 2207 88-89 1.1819445056296125 2207 90-91 1.2222464632753791 2207 92-93 1.2538075484829143 2207 94-95 1.26810171491422 2207 96-97 1.263771959156717 2207 98-99 1.2866309013473796 2207 100 1.4544470661438673 2208 1 0.7573552014360274 2208 2 0.7574718837471117 2208 3 0.7748155853812513 2208 4 0.6300177608807118 2208 5 0.61071176308063 2208 6 0.6579896144250625 2208 7 0.7290161629731671 2208 8 0.6503194229621769 2208 9 0.8390907436716688 2208 10-11 0.8530178801105635 2208 12-13 0.8909311161278097 2208 14-15 1.1163118194571453 2208 16-17 1.0970920319889785 2208 18-19 1.0551598231860098 2208 20-21 1.1343550427916185 2208 22-23 1.150240459531794 2208 24-25 1.15747054285373 2208 26-27 1.1673816307329261 2208 28-29 1.2474877881069872 2208 30-31 1.246013620979447 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1.3098295039565073 2208 100 1.440214937116398 2209 1 0.7126182601269733 2209 2 0.6971634305039522 2209 3 0.7415682727568189 2209 4 0.5721376621090073 2209 5 0.5468535116290028 2209 6 0.5818766967027003 2209 7 0.6342126338754781 2209 8 0.5979450243300448 2209 9 0.799162143946397 2209 10-11 0.8102221034584076 2209 12-13 0.8378478072096343 2209 14-15 1.0038552955182851 2209 16-17 1.011158457083397 2209 18-19 1.011226642009241 2209 20-21 1.034563073140042 2209 22-23 1.0466808732160118 2209 24-25 1.0517586090566056 2209 26-27 0.9919687941592699 2209 28-29 1.0259888937883446 2209 30-31 1.0885831468536722 2209 32-33 1.1430167781287253 2209 34-35 1.0882987913784987 2209 36-37 1.1470672104118407 2209 38-39 1.1849140052198024 2209 40-41 1.2223530648218812 2209 42-43 1.267962844322021 2209 44-45 1.23804861367406 2209 46-47 1.2564008855270359 2209 48-49 1.2857199568398698 2209 50-51 1.2507965479313015 2209 52-53 1.1785137182046128 2209 54-55 1.1037867163863027 2209 56-57 1.071660645217925 2209 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1.1509914516989248 2210 86-87 1.132244538059016 2210 88-89 1.0915612761952183 2210 90-91 1.1789273452217763 2210 92-93 1.1564309531113146 2210 94-95 1.229577771237782 2210 96-97 1.2489214980210868 2210 98-99 1.3031748106882972 2210 100 1.3627076144474302 2211 1 0.6757023836631859 2211 2 0.6625927218509453 2211 3 0.7015972932765671 2211 4 0.570562855076318 2211 5 0.5724157769468761 2211 6 0.6196920381260398 2211 7 0.6905214784619531 2211 8 0.5469943151200596 2211 9 0.748769609797101 2211 10-11 0.7832661482760273 2211 12-13 0.8364380319010962 2211 14-15 1.0373901828012322 2211 16-17 1.0307897842181077 2211 18-19 0.9785466547383592 2211 20-21 1.0245208825723466 2211 22-23 1.0656954925494162 2211 24-25 1.087636176635776 2211 26-27 1.1278523810885517 2211 28-29 1.1090853693210647 2211 30-31 1.1096624250306135 2211 32-33 1.1288304825789197 2211 34-35 1.1699885520433284 2211 36-37 1.1934265189089004 2211 38-39 1.203127021172385 2211 40-41 1.2488800071428727 2211 42-43 1.3035797667893192 2211 44-45 1.2977279317092538 2211 46-47 1.3412304552435828 2211 48-49 1.3618374890745812 2211 50-51 1.3935429192059772 2211 52-53 1.3095232995595438 2211 54-55 1.2566669318993462 2211 56-57 1.1691727680439996 2211 58-59 1.2176272487293502 2211 60-61 1.1842708627643432 2211 62-63 1.1524977046620322 2211 64-65 1.1688393026172648 2211 66-67 1.1299729154478015 2211 68-69 1.1577624734501697 2211 70-71 1.150090308384705 2211 72-73 1.1582250096540037 2211 74-75 1.153452997230076 2211 76-77 1.112816478156951 2211 78-79 1.126524909828177 2211 80-81 1.1381744229222832 2211 82-83 1.1423851006749892 2211 84-85 1.1329209055764942 2211 86-87 1.1880828624308144 2211 88-89 1.1898179653496648 2211 90-91 1.1766113653675525 2211 92-93 1.2353923616018179 2211 94-95 1.2562215496249642 2211 96-97 1.2551595373340696 2211 98-99 1.2668122448809953 2211 100 1.3095883903192806 2212 1 0.6786417778040033 2212 2 0.6597713410751886 2212 3 0.6982260605091177 2212 4 0.5310939069102432 2212 5 0.5475206761771432 2212 6 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70-71 0.9722119635186885 2212 72-73 0.9871148739095474 2212 74-75 1.0058367024121395 2212 76-77 1.0406069569350436 2212 78-79 1.0428480401707674 2212 80-81 1.0515035492079434 2212 82-83 1.074581520225376 2212 84-85 1.0124188183933889 2212 86-87 0.9895684917209486 2212 88-89 1.0721146104022417 2212 90-91 1.101424554725135 2212 92-93 1.1242606076146764 2212 94-95 1.1843911266047975 2212 96-97 1.1939177949460849 2212 98-99 1.2609680352631152 2212 100 1.3341683641076294 2213 1 0.6895325596783266 2213 2 0.6217341227846482 2213 3 0.6279209729418262 2213 4 0.5223896424847894 2213 5 0.5097161109421862 2213 6 0.536713117537424 2213 7 0.5559615812867307 2213 8 0.5448083210292509 2213 9 0.5928553674207748 2213 10-11 0.6644903425627788 2213 12-13 0.8008079854812209 2213 14-15 0.9786219902571673 2213 16-17 0.9678449259269897 2213 18-19 0.9248684663574807 2213 20-21 0.9752049067487221 2213 22-23 0.9779098328409361 2213 24-25 1.0153349020519258 2213 26-27 0.9964816699234476 2213 28-29 1.031930502421595 2213 30-31 1.03708641013489 2213 32-33 1.0718074687757024 2213 34-35 1.1099768268228942 2213 36-37 1.1612390610660626 2213 38-39 1.1681974747779478 2213 40-41 1.1896716246952934 2213 42-43 1.2060067927382292 2213 44-45 1.2178943571355774 2213 46-47 1.2570195226331222 2213 48-49 1.2693060349924394 2213 50-51 1.2167535307308839 2213 52-53 1.1682268914328517 2213 54-55 1.1095495731272322 2213 56-57 1.0713479049372907 2213 58-59 1.0317599887846782 2213 60-61 1.063508131162557 2213 62-63 1.0263234617770607 2213 64-65 1.0608665535610342 2213 66-67 1.0631531261285758 2213 68-69 1.0680721219672265 2213 70-71 1.0332111569406877 2213 72-73 1.0506489975226856 2213 74-75 1.0526305558796736 2213 76-77 1.0249601568214999 2213 78-79 1.0483401468647813 2213 80-81 0.9339638137471127 2213 82-83 1.05159213547552 2213 84-85 1.0818449165279524 2213 86-87 1.1028468230867112 2213 88-89 1.1015492501949815 2213 90-91 1.159249538582909 2213 92-93 1.2073271902198144 2213 94-95 1.2516264878460568 2213 96-97 1.267978345704467 2213 98-99 1.3788210862749644 2213 100 1.490456633009643 2214 1 0.5921552266371037 2214 2 0.539247868294094 2214 3 0.5850287804967813 2214 4 0.47209348383666594 2214 5 0.4834762435536888 2214 6 0.5165317005687484 2214 7 0.513495017914849 2214 8 0.5131078635934827 2214 9 0.6127972443064067 2214 10-11 0.663986516520346 2214 12-13 0.6746657158290361 2214 14-15 0.8713166116254598 2214 16-17 0.8616557512910532 2214 18-19 0.8337231348155285 2214 20-21 0.864883170762802 2214 22-23 0.8461281429417369 2214 24-25 0.904118355177296 2214 26-27 0.9195513595903648 2214 28-29 0.8673681150978254 2214 30-31 0.9798788931955116 2214 32-33 0.9755935218678147 2214 34-35 1.0535196048543654 2214 36-37 1.0191911319329705 2214 38-39 0.9601549329871091 2214 40-41 1.0324567829765172 2214 42-43 1.090972122115737 2214 44-45 1.129435107984854 2214 46-47 1.1961390577537898 2214 48-49 1.1939931151374026 2214 50-51 1.2181864819438957 2214 52-53 1.117598808096659 2214 54-55 1.0216340733661085 2214 56-57 1.0027345186719785 2214 58-59 0.9902463066246767 2214 60-61 0.946899952749412 2214 62-63 0.9576658628793311 2214 64-65 0.9413875235466023 2214 66-67 0.9137230071635969 2214 68-69 0.9314303419534511 2214 70-71 0.9174565462890101 2214 72-73 0.9062835345834372 2214 74-75 0.9542726262450714 2214 76-77 0.9627648610455921 2214 78-79 1.0042918270767913 2214 80-81 1.0155908914108949 2214 82-83 1.0015434355908717 2214 84-85 1.0288837919697507 2214 86-87 1.048136330043267 2214 88-89 1.0957369387585523 2214 90-91 1.137957526683767 2214 92-93 1.1773919386301408 2214 94-95 1.2581079277789087 2214 96-97 1.2542945359745197 2214 98-99 1.3647235254924936 2214 100 1.429247212947402 2215 1 0.584025601235787 2215 2 0.516548060186981 2215 3 0.5574349264778284 2215 4 0.42672419708510034 2215 5 0.38150845079689333 2215 6 0.46463079848911804 2215 7 0.5119487154289573 2215 8 0.5191395144103694 2215 9 0.6223076167547461 2215 10-11 0.6700256531626607 2215 12-13 0.6775995801535473 2215 14-15 0.8396681306928357 2215 16-17 0.8468812799346637 2215 18-19 0.776838572156251 2215 20-21 0.8607555910777265 2215 22-23 0.8440057167711004 2215 24-25 0.897644917215537 2215 26-27 0.9212408486659669 2215 28-29 0.9255512073346139 2215 30-31 0.927955451343017 2215 32-33 0.9358851777293609 2215 34-35 1.0004716939004012 2215 36-37 1.077063836209156 2215 38-39 1.1080071480684879 2215 40-41 1.094807270626923 2215 42-43 1.1495059086560317 2215 44-45 1.174036038853103 2215 46-47 1.1669196907706336 2215 48-49 1.1917379825326364 2215 50-51 1.2090417121448382 2215 52-53 1.1151362912230098 2215 54-55 0.9724984668504177 2215 56-57 0.9444525027557162 2215 58-59 0.9645192370468507 2215 60-61 0.9264186742512024 2215 62-63 0.9510472157306182 2215 64-65 0.9211252457990113 2215 66-67 0.9382088477548791 2215 68-69 0.9036703279321401 2215 70-71 0.9438200154812009 2215 72-73 0.9308891293899677 2215 74-75 0.9601008968392293 2215 76-77 0.978594985718864 2215 78-79 0.9955362600599216 2215 80-81 0.9924959709925929 2215 82-83 1.0565838453742593 2215 84-85 1.0336404816895133 2215 86-87 1.060838686939423 2215 88-89 1.0558843033567804 2215 90-91 1.1199952426841797 2215 92-93 1.1321097659958248 2215 94-95 0.9390958658066815 2215 96-97 1.0863821129531779 2215 98-99 1.2176158920725477 2215 100 1.2877619915051035 2216 1 0.48723210575507636 2216 2 0.45289246598344945 2216 3 0.47164020154615827 2216 4 0.3408634721043242 2216 5 0.3344164875383697 2216 6 0.38138273442387316 2216 7 0.3801477632550174 2216 8 0.35841663014804936 2216 9 0.47730473429382414 2216 10-11 0.3739928610606782 2216 12-13 0.4510995393916275 2216 14-15 0.6725752366479796 2216 16-17 0.620893962250598 2216 18-19 0.6673324877807261 2216 20-21 0.6408063848691583 2216 22-23 0.7120107142103151 2216 24-25 0.6624793452737308 2216 26-27 0.6118730571188351 2216 28-29 0.718169415519057 2216 30-31 0.7966843272063002 2216 32-33 0.8855330072927785 2216 34-35 0.7762813443814949 2216 36-37 0.843583333222206 2216 38-39 0.6210046030336187 2216 40-41 0.8638854218227934 2216 42-43 0.8747329742711187 2216 44-45 0.8069816469707405 2216 46-47 0.8631488954338735 2216 48-49 0.7116070412519093 2216 50-51 0.8902949807396539 2216 52-53 0.765511788932038 2216 54-55 0.5628141791415402 2216 56-57 0.6466127496586367 2216 58-59 0.6347545496273383 2216 60-61 0.6853733877509782 2216 62-63 0.3074132997585437 2216 64-65 0.4062462164952123 2216 66-67 0.5707521724500637 2216 68-69 0.6992371315206327 2216 70-71 0.6160960579018848 2216 72-73 0.6776881414121192 2216 74-75 0.7197639873936339 2216 76-77 0.7773149144464924 2216 78-79 0.8006432683209042 2216 80-81 0.688367107785183 2216 82-83 0.5843785689616077 2216 84-85 0.6529125312352555 2216 86-87 0.783841118794772 2216 88-89 0.9160554822620135 2216 90-91 0.9904400077968205 2216 92-93 1.040513803188741 2216 94-95 1.076061577884957 2216 96-97 1.1422128091919213 2216 98-99 1.218103277789158 2216 100 1.0611024207506006 2301 1 0.7233683332926972 2301 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1.337153210032195 2310 98-99 1.3724389627005138 2310 100 1.4535877604521268 2311 1 0.7628913628565712 2311 2 0.7375825767835771 2311 3 0.7273922976467482 2311 4 0.6338962404356394 2311 5 0.6277128145871984 2311 6 0.6457461673812972 2311 7 0.6650116356182707 2311 8 0.5830550634996854 2311 9 0.8022108804987127 2311 10-11 0.8449937042654483 2311 12-13 0.8934775735426967 2311 14-15 1.0596982636666397 2311 16-17 1.0733724807060696 2311 18-19 1.021082527838466 2311 20-21 1.0649745870117684 2311 22-23 1.0935482429268362 2311 24-25 1.1470292497540484 2311 26-27 1.1178845154120793 2311 28-29 1.1708484685185354 2311 30-31 1.1738725605914127 2311 32-33 1.1590617014342826 2311 34-35 1.2230440264068179 2311 36-37 1.2968423205252932 2311 38-39 1.300504485785261 2311 40-41 1.2956049949404118 2311 42-43 1.3358994861043882 2311 44-45 1.3924575337388276 2311 46-47 1.3806495401727048 2311 48-49 1.3245442746348175 2311 50-51 1.3621329925058632 2311 52-53 1.3177828210245153 2311 54-55 1.1874879480803742 2311 56-57 1.1179224062269029 2311 58-59 1.0604629866091884 2311 60-61 1.094599197582788 2311 62-63 1.133018714174277 2311 64-65 1.1516991758252075 2311 66-67 1.1443579470039253 2311 68-69 1.1508026557208453 2311 70-71 1.0936605881615478 2311 72-73 1.0865213938952323 2311 74-75 1.0872996201286966 2311 76-77 1.07182265529692 2311 78-79 1.1733088347915697 2311 80-81 1.1657203027479355 2311 82-83 1.2151793998565772 2311 84-85 1.2181135057785433 2311 86-87 1.2196628076900176 2311 88-89 1.2586760670727664 2311 90-91 1.2904810913514133 2311 92-93 1.3141007739200568 2311 94-95 1.3338963711285352 2311 96-97 1.3546100897081566 2311 98-99 1.4701707233195336 2311 100 1.5448518995761233 2312 1 0.627748328255862 2312 2 0.6187689123325022 2312 3 0.6514593444707124 2312 4 0.3437218883689752 2312 5 0.4558076414914609 2312 6 0.49693343838677606 2312 7 0.3938071086114192 2312 8 0.523900520645455 2312 9 0.7199187645117746 2312 10-11 0.6775836329559013 2312 12-13 0.7900992715852411 2312 14-15 1.0007608701037398 2312 16-17 0.9994690301261571 2312 18-19 0.941924527755539 2312 20-21 0.9834229081897163 2312 22-23 1.060712715710146 2312 24-25 1.0438044131408688 2312 26-27 1.0624790714054626 2312 28-29 1.0606615464954814 2312 30-31 1.115790077577799 2312 32-33 1.1182559898008648 2312 34-35 1.1732411539839163 2312 36-37 1.1494945536360248 2312 38-39 1.1898175974617686 2312 40-41 1.2546026271383184 2312 42-43 1.2649905794890586 2312 44-45 1.2951128108511014 2312 46-47 1.2875815570860638 2312 48-49 1.3136820539254614 2312 50-51 1.3330107160679745 2312 52-53 1.1936862910215496 2312 54-55 1.1486500108276019 2312 56-57 1.0645079740565677 2312 58-59 1.0471950373307664 2312 60-61 1.033232676769991 2312 62-63 1.0241680509972682 2312 64-65 1.0856366771636417 2312 66-67 1.0486792795875388 2312 68-69 1.0410984839526591 2312 70-71 1.0489427825260051 2312 72-73 1.0495970032834947 2312 74-75 1.0871237725232277 2312 76-77 1.09644585189249 2312 78-79 1.1173300319775734 2312 80-81 1.0286738085947817 2312 82-83 1.0580452433658785 2312 84-85 1.134529800835768 2312 86-87 1.15230804362783 2312 88-89 1.071530603439431 2312 90-91 1.0975294988268907 2312 92-93 1.2508268961112918 2312 94-95 1.304354842623379 2312 96-97 1.3050726990823556 2312 98-99 0.9583721137605608 2312 100 1.1813222275007824 2313 1 0.6633956662788094 2313 2 0.6415215950414925 2313 3 0.6216555980296725 2313 4 0.5384403445932833 2313 5 0.3637963091465437 2313 6 0.5143742376323175 2313 7 0.5899207309831951 2313 8 0.5480620470103901 2313 9 0.7278099635086264 2313 10-11 0.7286690969747909 2313 12-13 0.8189332377980563 2313 14-15 0.9594960413686877 2313 16-17 1.0288087560402772 2313 18-19 0.9593108145609577 2313 20-21 1.0306285915334144 2313 22-23 1.028497605100199 2313 24-25 0.9922471621547686 2313 26-27 1.0196097811716314 2313 28-29 1.098486076613959 2313 30-31 1.0716312333977314 2313 32-33 1.104547445704462 2313 34-35 1.1370579382788364 2313 36-37 1.198780953074582 2313 38-39 1.218275817331893 2313 40-41 1.2596155847021322 2313 42-43 1.3046661781916882 2313 44-45 1.270442014983466 2313 46-47 1.2759837365467988 2313 48-49 1.3172426402677928 2313 50-51 1.2908678375055587 2313 52-53 1.1913764230749422 2313 54-55 1.107138420951003 2313 56-57 1.0399218588456094 2313 58-59 1.0665173379517299 2313 60-61 1.0223564151328688 2313 62-63 1.0141497951404261 2313 64-65 1.0931468014838188 2313 66-67 1.072900305123774 2313 68-69 1.114361960985498 2313 70-71 1.1461807315944554 2313 72-73 1.1517089579503619 2313 74-75 1.098955925890138 2313 76-77 0.858444940188086 2313 78-79 1.0299433418798998 2313 80-81 1.117493000017312 2313 82-83 1.148644259092979 2313 84-85 1.1536144832138469 2313 86-87 1.180445843413846 2313 88-89 1.261691592603814 2313 90-91 1.3329465637371207 2313 92-93 1.378708763725104 2313 94-95 1.3613187685028834 2313 96-97 1.364013209134331 2313 98-99 1.4290450238012617 2313 100 1.5940249435652802 2314 1 0.5872419213558544 2314 2 0.5518887752844499 2314 3 0.624480924139899 2314 4 0.509282132471796 2314 5 0.4707038366855798 2314 6 0.4725534442738848 2314 7 0.5463493889370028 2314 8 0.4989388565249371 2314 9 0.6623312918666215 2314 10-11 0.6648728933975363 2314 12-13 0.7488072738367606 2314 14-15 0.8585433470355852 2314 16-17 0.9114107014914623 2314 18-19 0.8904157020605865 2314 20-21 0.8983354720695971 2314 22-23 0.9297739390771227 2314 24-25 0.9032307899298857 2314 26-27 0.9708688234995932 2314 28-29 1.0013178000913214 2314 30-31 0.983494311575825 2314 32-33 0.9969043394154653 2314 34-35 1.040193270137813 2314 36-37 1.0387869970069232 2314 38-39 1.0801433825522153 2314 40-41 1.132300258575114 2314 42-43 1.1898408722213176 2314 44-45 1.2364775438675366 2314 46-47 1.2772898656687701 2314 48-49 1.2217688861081442 2314 50-51 1.255570877745697 2314 52-53 1.1769431236733254 2314 54-55 1.0517066268064283 2314 56-57 1.0320539956738415 2314 58-59 1.0264367962842798 2314 60-61 0.9781433618778479 2314 62-63 0.9761125903041332 2314 64-65 1.045574548010265 2314 66-67 1.0534410445547664 2314 68-69 1.0001107199900687 2314 70-71 1.0456243975938406 2314 72-73 1.0774678283490786 2314 74-75 1.0444961563947714 2314 76-77 1.038320412672654 2314 78-79 1.0532751321445915 2314 80-81 1.040705678809168 2314 82-83 1.046376303593771 2314 84-85 1.0890597473703103 2314 86-87 1.145815266190663 2314 88-89 1.1944236030445587 2314 90-91 1.2550511751034925 2314 92-93 1.305970966036508 2314 94-95 1.2985877857873263 2314 96-97 1.3586261323885331 2314 98-99 1.415960828427643 2314 100 1.4620825242133222 2315 1 0.39447577326857086 2315 2 0.462739576124779 2315 3 0.5201484140307464 2315 4 0.1299519834795717 2315 5 0.24826454922243357 2315 6 0.29843760626901883 2315 7 0.3356812227067536 2315 8 0.3930931182212234 2315 9 0.5291673223207312 2315 10-11 0.4204744406888139 2315 12-13 0.4958665035134189 2315 14-15 0.6792999210972397 2315 16-17 0.6794874225980436 2315 18-19 0.6233309552566553 2315 20-21 0.7107330752356305 2315 22-23 0.5319302311698948 2315 24-25 0.6973543751548519 2315 26-27 0.8056029243222724 2315 28-29 0.797728485584436 2315 30-31 0.8372372217438411 2315 32-33 0.8193389604320771 2315 34-35 0.8753129861500852 2315 36-37 0.8996879703515361 2315 38-39 0.92693017641664 2315 40-41 0.9789912984005156 2315 42-43 1.0705222167328614 2315 44-45 1.0703010641101045 2315 46-47 1.1134452670018788 2315 48-49 1.1364232970191495 2315 50-51 0.9854453634603004 2315 52-53 1.0336949762887002 2315 54-55 0.9543266396401364 2315 56-57 0.9227755379140703 2315 58-59 0.8982456212238219 2315 60-61 0.8500452925987716 2315 62-63 0.869958531805004 2315 64-65 0.8866911958503465 2315 66-67 0.8807141766585858 2315 68-69 0.9244662006987099 2315 70-71 0.9020094255068685 2315 72-73 0.6235572542665508 2315 74-75 0.8192456925887086 2315 76-77 0.8409720536439949 2315 78-79 0.9164438344255004 2315 80-81 0.5836333377960159 2315 82-83 0.5853182626467905 2315 84-85 0.8709163866506522 2315 86-87 1.0204871529767594 2315 88-89 1.0127203165863712 2315 90-91 1.0341366802213834 2315 92-93 1.1126546366830468 2315 94-95 1.098831002162182 2315 96-97 0.9354209209062923 2315 98-99 0.4629293675863835 2315 100 0.935088752114563 2316 1 0.27058067541647546 2316 2 0.20312243425565768 2316 3 0.30690091420518684 2316 4 0.19171020434866648 2316 5 0.1020437221998165 2316 6 0.17221499377399851 2316 7 0.08776680070727139 2316 8 0.1873161273168762 2316 9 0.2714393949668832 2316 10-11 0.2152275567999382 2316 12-13 0.0056049151304833345 2316 14-15 0.042311570535126464 2316 16-17 0.2462003871287095 2316 18-19 0.3082980407397429 2316 20-21 0.2966330187469808 2316 22-23 0.291352760394183 2316 24-25 0.32956813263023577 2316 26-27 0.42159047013913664 2316 28-29 0.40709190951510266 2316 30-31 0.23538033043601558 2316 32-33 0.421163079599026 2316 34-35 0.4031280617453419 2316 36-37 0.43158378675367004 2316 38-39 0.6373829116782801 2316 40-41 0.6698822593951235 2316 42-43 0.5720991274961804 2316 44-45 0.5476586316399548 2316 46-47 0.36126792059355495 2316 48-49 0.32803638027347404 2316 50-51 0.32955621615677444 2316 52-53 0.5165572565935008 2316 54-55 0.24372189551680634 2316 56-57 0.08479560241682549 2316 58-59 0.1523000224646225 2316 60-61 0.23079286553635114 2316 62-63 0.38368381866064993 2316 64-65 0.30616172118968876 2316 66-67 0.2770265796889575 2316 68-69 0.19794893394912805 2316 70-71 -0.03434857761667587 2316 72-73 -0.056939794573303004 2316 74-75 0.19089344248301643 2316 76-77 0.31149111433158794 2316 78-79 0.30663903837980655 2316 80-81 0.47193005355291007 2316 82-83 0.5587951405225304 2316 84-85 0.6227316297622174 2316 86-87 0.6029206569561083 2316 88-89 0.6101380450447458 2316 90-91 0.42780246030864433 2316 92-93 0.4919661228630794 2316 94-95 0.6405564855223993 2316 96-97 0.6032440401060732 2316 98-99 0.4804481163293701 2316 100 0.39011073959163056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 30.0 1 696.0 2 249.0 3 217.0 4 179.0 5 156.0 6 176.0 7 685.0 8 2266.0 9 4277.0 10 6885.0 11 10614.0 12 14885.0 13 19132.0 14 22839.0 15 26972.0 16 32093.0 17 39513.0 18 48134.0 19 58045.0 20 72153.0 21 88232.0 22 98498.0 23 99881.0 24 98035.0 25 102955.0 26 114664.0 27 132107.0 28 156327.0 29 188258.0 30 229997.0 31 287054.0 32 366138.0 33 482676.0 34 691592.0 35 970333.0 36 1478828.0 37 2093572.0 38 1810003.0 39 511065.0 40 407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55184301968541 20.094116492723636 12.987807522037789 23.366232965553166 2 15.5839486708796 19.424316018769055 41.31771713064356 23.674018179707783 3 19.479494963614304 25.510029137902674 28.514928444941102 26.495547453541917 4 12.678060574116628 15.942376927602883 38.673873894746094 32.7056886035344 5 13.437380837226184 37.90822813221312 34.4549573161961 14.199433714364599 6 30.979165529958635 37.23067503798818 17.574792169534227 14.215367262518958 7 27.941821536314894 31.796448001675 22.201322269417464 18.060408192592643 8 26.36980818253049 35.591769224960395 19.441500780159554 18.596921812349564 9 26.18007276603634 15.779434711407736 21.20174035965888 36.83875216289705 10-11 25.973824458304346 26.13175792212803 26.922115623826077 20.972301995741546 12-13 25.929662011661907 23.568771428670747 27.722457459369288 22.779109100298058 14-15 23.244411332492984 24.90256711746706 25.43590604990589 26.417115500134063 16-17 22.175829661344228 27.85898808859844 26.51934819009724 23.445834059960088 18-19 21.680545491790383 27.649714555901728 28.75335000298293 21.91638994932496 20-21 22.982455513698692 26.75946379533415 28.41155285540048 21.846527835566683 22-23 23.279883689424814 26.487513985995253 28.057845772250673 22.17475655232926 24-25 22.699883932172366 26.43862439953785 28.731838908764505 22.12965275952527 26-27 22.60202866058434 26.784133152668137 27.93849956500568 22.67533862174184 28-29 22.016676740169935 27.043132839244166 28.407071666869875 22.53311875371602 30-31 22.803134340259255 26.742620513417535 28.33869772278434 22.115547423538867 32-33 22.902004973382805 26.947063535843863 27.695778919058668 22.455152571714663 34-35 22.94061131147008 26.860018190150498 28.267495807489855 21.931874690889565 36-37 22.63186295396129 26.536144698988746 27.989415504396764 22.842576842653205 38-39 22.75769242279581 26.739805784003007 28.334789454528952 22.16771233867223 40-41 22.630395089182063 26.002411810631447 28.707333412304635 22.659859687881852 42-43 22.531887198468194 26.18284446129973 28.259523967680067 23.025744372552005 44-45 22.652194996378093 26.85619197680125 28.115739438095293 22.375873588725366 46-47 22.645502093706753 26.729292465784376 27.568938206198734 23.056267234310138 48-49 23.119418686444178 26.707738922584706 27.558428027879955 22.614414363091164 50-51 22.870858482352865 26.550529224594793 27.613916701282143 22.964695591770198 52-53 22.552815945190517 26.971011970383145 27.371008011555915 23.105164072870423 54-55 22.405137735220364 26.903456108693142 27.444576519100195 23.246829636986302 56-57 23.10032810489083 26.65806615570195 27.08927772605091 23.152328013356318 58-59 22.438986778813135 26.906210988113084 27.681725718707074 22.973076514366706 60-61 22.529387101504668 27.337469376217555 27.22692110564489 22.906222416632886 62-63 22.757874580751096 26.80110371518657 26.997770924745225 23.44325077931711 64-65 22.239202383896917 26.909324488524323 27.2777294745035 23.573743653075265 66-67 22.612345963176566 26.645944658450855 27.20122258174956 23.54048679662302 68-69 22.295980584193842 26.708720857619305 27.406953738668044 23.58834481951881 70-71 22.33645835314774 26.448529385495377 27.427081932243464 23.787930329113415 72-73 22.832048666851744 26.550171999662037 27.48314644715084 23.134632886335382 74-75 22.97479693120427 26.778410841586332 27.16330157995201 23.083490647257392 76-77 22.766062724393134 26.764630682222705 26.867684815762622 23.60162177762154 78-79 22.70135331888748 26.774941059103853 26.911971040331196 23.611734581677478 80-81 22.42355194795102 27.15453286817079 27.239513496642235 23.182401687235952 82-83 23.141290703559818 26.452109322138373 27.99317038313759 22.413429591164224 84-85 23.2092248840352 26.728993668276413 27.104682595007105 22.95709885268128 86-87 22.328035846603598 27.238414511009097 27.427057474806094 23.00649216758121 88-89 23.080689451681963 26.33885869495701 27.312850113615195 23.267601739745828 90-91 22.936671956171526 26.48185016019356 27.285571719048857 23.29590616458606 92-93 23.19395149587162 26.57829608687902 27.227289956926654 23.000462460322705 94-95 22.43083031140449 26.57329077492111 27.700733106864657 23.295145806809746 96-97 22.689911690759086 26.50867754323204 27.33588670428098 23.46552406172789 98-99 22.30751169538927 26.837466914232266 27.221419187312367 23.633602203066097 100 22.99317521141011 26.4150695657295 26.542509386618622 24.049245836241763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 725.0 1 567.0 2 611.5 3 869.5 4 784.5 5 701.5 6 1012.0 7 1839.0 8 2973.0 9 3703.5 10 3737.5 11 3744.5 12 4296.5 13 5182.0 14 6120.5 15 7158.5 16 8241.5 17 9218.0 18 10076.5 19 10948.0 20 12049.0 21 13306.0 22 14874.5 23 17282.5 24 20190.0 25 23897.5 26 29861.0 27 37036.0 28 46907.0 29 64141.5 30 87103.5 31 110113.0 32 141092.0 33 174977.0 34 202644.5 35 229848.5 36 256472.5 37 295030.0 38 315765.0 39 312102.0 40 302676.5 41 289916.0 42 290989.5 43 305664.0 44 328925.5 45 370151.0 46 411619.0 47 440825.0 48 462688.0 49 462237.0 50 448979.5 51 457688.0 52 458640.0 53 436847.0 54 412377.5 55 362653.0 56 316532.5 57 265283.5 58 215818.5 59 198527.0 60 165524.5 61 121960.5 62 93846.0 63 76427.5 64 56870.0 65 33782.5 66 22124.5 67 16028.5 68 12091.5 69 9218.0 70 6909.5 71 5034.0 72 3857.0 73 3052.5 74 2310.5 75 1714.5 76 1199.5 77 798.5 78 540.5 79 371.5 80 262.5 81 209.5 82 162.5 83 122.5 84 94.0 85 78.0 86 71.0 87 53.5 88 40.0 89 37.0 90 36.0 91 28.0 92 20.5 93 18.5 94 15.5 95 8.0 96 7.5 97 6.5 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057823619718057016 2 0.13306864380785377 3 0.01739244912901665 4 0.017112548449359884 5 0.02015284893528677 6 0.05427177661068846 7 0.059802227970803076 8 0.05061376428000183 9 0.04186928097762165 10-11 0.040937887336694845 12-13 0.04213952990970404 14-15 0.019501355974016723 16-17 0.01812115607088166 18-19 0.018068071459222623 20-21 0.021581307576293687 22-23 0.02594872335369659 24-25 0.025581956945870492 26-27 0.01990190349835312 28-29 0.018907773498192903 30-31 0.01702568272119055 32-33 0.023897726994142738 34-35 0.02379155777082466 36-37 0.023738473159165618 38-39 0.02169712854718614 40-41 0.02533583738272403 42-43 0.03252638932563046 44-45 0.04246768932723266 46-47 0.04847107631849145 48-49 0.041241917385287534 50-51 0.028718774907541086 52-53 0.04068694189976119 54-55 0.03745843233613408 56-57 0.03927296088011584 58-59 0.04439321296831968 60-61 0.02361782631448598 62-63 0.02616588767411994 64-65 0.025596434567232047 66-67 0.032738727772266635 68-69 0.039586642676282896 70-71 0.04147355932707244 72-73 0.04529565136652338 74-75 0.027155191800492976 76-77 0.021084242576213576 78-79 0.02061613281885658 80-81 0.024853250004005477 82-83 0.020374839129497304 84-85 0.012706525681659498 86-87 0.015114636701465078 88-89 0.018806430148662005 90-91 0.02176951665399392 92-93 0.020186630051797067 94-95 0.024182453547586687 96-97 0.025244145780767502 98-99 0.027893550489932358 100 0.02883942175222072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0360818E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.80333748437571 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30588848146022 46.541552403804474 2 14.426910324443472 17.832624152768084 3 4.934628238566644 9.14929483164194 4 2.117389138361838 5.234468620157127 5 1.0833381554374506 3.3476956815100913 6 0.5910673346029371 2.191796037387345 7 0.3816342723146427 1.6510390209226202 8 0.24365831839739896 1.2047117826231937 9 0.17574083527667003 0.9775233137151106 >10 0.6895958706917917 7.0572715612043595 >50 0.03217100351442661 1.3604920970049048 >100 0.0164216203992463 1.8215242524823845 >500 0.0011358569842228052 0.45715898553024836 >1k 3.3643965076346916E-4 0.30086304984360357 >5k 2.102747458862724E-5 0.07261986589991017 >10k+ 6.308242376588172E-5 0.7993643435045588 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26907 0.25969957198360205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21787 0.21028262440282225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12816 0.12369679691313948 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.651747574371059E-6 0.0 10-11 0.0 9.651747574371059E-6 0.0 9.651747574371059E-6 2.8955242723113174E-5 12-13 0.0 9.651747574371059E-6 0.0 1.4477621361556589E-5 3.8606990297484237E-5 14-15 0.0 9.651747574371059E-6 0.0 2.8955242723113174E-5 3.8606990297484237E-5 16-17 0.0 9.651747574371059E-6 0.0 3.860699029748423E-5 3.8606990297484237E-5 18-19 0.0 9.651747574371059E-6 0.0 6.756223302059741E-5 6.273635923341187E-5 20-21 0.0 9.651747574371059E-6 0.0 9.169160195652504E-5 7.721398059496847E-5 22-23 0.0 9.651747574371059E-6 0.0 2.3646781557209093E-4 8.2039854382154E-5 24-25 0.0 9.651747574371059E-6 0.0 5.211943690160371E-4 8.686572816933953E-5 26-27 0.0 9.651747574371059E-6 0.0 0.001138906213775785 9.169160195652504E-5 28-29 0.0 9.651747574371059E-6 0.0 0.004227465437574523 1.0134334953089611E-4 30-31 0.0 9.651747574371059E-6 0.0 0.016301801653112718 1.0616922331808164E-4 32-33 0.0 9.651747574371059E-6 0.0 0.04386719272551646 1.2547271846682375E-4 34-35 0.0 9.651747574371059E-6 0.0 0.08530214506229142 1.2547271846682375E-4 36-37 0.0 9.651747574371059E-6 0.0 0.15532557371435346 1.3029859225400928E-4 38-39 0.0 9.651747574371059E-6 0.0 0.2569343463035447 1.3512446604119481E-4 40-41 0.0 9.651747574371059E-6 0.0 0.3622976486991664 1.3512446604119481E-4 42-43 0.0 9.651747574371059E-6 0.0 0.4666909504635638 1.9303495148742116E-4 44-45 0.0 9.651747574371059E-6 0.0 0.588481527230765 2.0268669906179222E-4 46-47 0.0 9.651747574371059E-6 0.0 0.734719980603848 2.0268669906179222E-4 48-49 0.0 9.651747574371059E-6 0.0 0.8943791889790942 2.123384466361633E-4 50-51 0.0 9.651747574371059E-6 0.0 1.059259992792075 2.123384466361633E-4 52-53 0.0 9.651747574371059E-6 0.0 1.2166606922349181 2.123384466361633E-4 54-55 0.0 9.651747574371059E-6 0.0 1.387211897747842 2.123384466361633E-4 56-57 0.0 9.651747574371059E-6 0.0 1.5902894925863962 2.123384466361633E-4 58-59 0.0 9.651747574371059E-6 0.0 1.8193254625262214 2.2199019421053435E-4 60-61 0.0 9.651747574371059E-6 0.0 2.0516864595054174 2.2199019421053435E-4 62-63 0.0 9.651747574371059E-6 0.0 2.2859343731354027 2.2199019421053435E-4 64-65 0.0 9.651747574371059E-6 0.0 2.5252735836108693 2.2199019421053435E-4 66-67 0.0 9.651747574371059E-6 0.0 2.7923422648675036 2.2199019421053435E-4 68-69 4.8258737871855296E-6 9.651747574371059E-6 0.0 3.0862814113711874 2.2199019421053435E-4 70-71 9.651747574371059E-6 9.651747574371059E-6 0.0 3.4020142038978003 2.316419417849054E-4 72-73 9.651747574371059E-6 9.651747574371059E-6 0.0 3.7188665991430407 2.316419417849054E-4 74-75 9.651747574371059E-6 9.651747574371059E-6 0.0 4.026284411134333 2.316419417849054E-4 76-77 9.651747574371059E-6 9.651747574371059E-6 0.0 4.366831846674654 2.316419417849054E-4 78-79 9.651747574371059E-6 9.651747574371059E-6 0.0 4.73991532328818 2.316419417849054E-4 80-81 9.651747574371059E-6 9.651747574371059E-6 0.0 5.138093343595071 2.4611956314646196E-4 82-83 9.651747574371059E-6 9.651747574371059E-6 0.0 5.539060718950955 2.6059718450801856E-4 84-85 9.651747574371059E-6 9.651747574371059E-6 0.0 5.932186049402663 2.6059718450801856E-4 86-87 9.651747574371059E-6 2.8955242723113174E-5 0.0 6.342105420633777 2.6059718450801856E-4 88 9.651747574371059E-6 2.8955242723113174E-5 0.0 6.67374911903674 2.6059718450801856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16945 0.0 45.355938 1 GTATCAA 29740 0.0 39.768673 1 GTACTAG 8400 0.0 37.875378 1 CAACGCA 33320 0.0 33.747375 5 ATCAACG 33940 0.0 33.172607 3 GCATCGT 8570 0.0 32.689587 8 TATCAAC 37270 0.0 31.291262 2 AACGCAG 38245 0.0 30.077888 6 GGGCACC 10105 0.0 29.16082 94 CTGTGCG 11535 0.0 28.932838 9 TCAACGC 39730 0.0 28.669128 4 TAGGCAT 11155 0.0 28.57222 5 TAGTACT 12160 0.0 28.414066 4 CTAACGC 4010 0.0 28.135382 3 TCTAACG 4125 0.0 28.066763 2 CTAGGCA 12055 0.0 27.413704 4 TAACGCC 4100 0.0 27.402721 4 GTGGTAT 6240 0.0 27.3032 1 ACGCAGA 41075 0.0 27.28494 7 GTACATG 42230 0.0 26.99247 1 >>END_MODULE