FastQCFastQC Report
Mon 6 Jun 2016
ERR1211201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246240.23766463227131293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212210.20481973527572825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127480.12304047807808224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176550.045.3531271
GTATCAA300950.037.9327771
CAACGCA339250.032.0223775
ATCAACG345400.031.434713
GGGCACC97650.029.99268294
TATCAAC380050.029.9374752
AACGCAG387750.029.034926
GTGGTAT65350.028.9947761
CTGTGCG115050.028.4250979
TAGTACT120250.027.8691844
TCAACGC404550.027.303324
TAGGCAT109250.027.2255
ACGCAGA418300.025.9371177
CTAGGCA119700.025.8768314
GCATCGT103950.025.674578
CTAGTAC133750.025.618493
CGCAGAG426250.025.4199268
GTACTGT138250.025.2527876
GTACATG427500.024.6141191
TACCTGG56950.024.5914212
Produced by FastQC (version 0.11.5)