##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10360818 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16880134367769 33.0 31.0 34.0 30.0 34.0 2 32.316218661499505 34.0 31.0 34.0 31.0 34.0 3 32.39522815669574 34.0 31.0 34.0 31.0 34.0 4 36.00240965529942 37.0 35.0 37.0 35.0 37.0 5 35.885897233210734 37.0 35.0 37.0 35.0 37.0 6 35.91256452917135 37.0 35.0 37.0 35.0 37.0 7 35.851407967980904 37.0 35.0 37.0 35.0 37.0 8 35.840175167636374 37.0 35.0 37.0 35.0 37.0 9 37.449633223940424 39.0 37.0 39.0 34.0 39.0 10-11 37.430886441591774 39.0 37.0 39.0 34.5 39.0 12-13 37.32880396123163 39.0 37.0 39.0 34.0 39.0 14-15 38.56022796655631 40.0 38.0 41.0 34.0 41.0 16-17 38.511006322087695 40.0 38.0 41.0 34.0 41.0 18-19 38.53072199511661 40.0 38.0 41.0 34.0 41.0 20-21 38.491526682545725 40.0 38.0 41.0 34.0 41.0 22-23 38.360438818633824 40.0 38.0 41.0 34.0 41.0 24-25 38.293770047886184 40.0 38.0 41.0 34.0 41.0 26-27 38.15693471306996 40.0 38.0 41.0 33.5 41.0 28-29 38.14041067992894 40.0 38.0 41.0 33.5 41.0 30-31 38.16677631051911 40.0 37.5 41.0 34.0 41.0 32-33 38.05139772747673 40.0 37.0 41.0 33.0 41.0 34-35 37.919156962317075 40.0 37.0 41.0 33.0 41.0 36-37 37.784623038451215 40.0 37.0 41.0 33.0 41.0 38-39 37.69435801304492 40.0 37.0 41.0 33.0 41.0 40-41 37.59550515219937 40.0 37.0 41.0 32.5 41.0 42-43 37.454981257271385 40.0 36.0 41.0 32.0 41.0 44-45 37.30832367675988 40.0 36.0 41.0 31.5 41.0 46-47 37.07169573869554 39.0 36.0 41.0 31.0 41.0 48-49 36.83075771623437 39.0 35.0 41.0 31.0 41.0 50-51 36.62522534417649 39.0 35.0 41.0 30.5 41.0 52-53 36.4099419080617 39.0 35.0 40.0 30.0 41.0 54-55 36.1634182262443 38.0 35.0 40.0 29.5 41.0 56-57 35.890026829927905 38.0 35.0 40.0 29.0 41.0 58-59 35.6019501549009 37.5 34.0 40.0 28.5 41.0 60-61 35.332236026151605 37.0 34.0 40.0 28.0 41.0 62-63 35.023105608070715 36.5 34.0 40.0 28.0 41.0 64-65 34.643653088009074 36.0 34.0 39.0 27.0 41.0 66-67 34.34097273979719 35.5 33.0 39.0 26.5 40.0 68-69 34.017213167917824 35.0 33.0 38.5 26.0 40.0 70-71 33.713164201899886 35.0 33.0 38.0 26.0 40.0 72-73 33.389895807454586 35.0 32.5 37.0 26.0 39.5 74-75 33.067736929651694 35.0 32.0 37.0 26.0 39.0 76-77 32.088081848363714 34.0 30.5 35.5 24.5 38.0 78-79 32.5990774570116 35.0 32.0 36.0 26.0 38.0 80-81 32.53565162519021 35.0 32.0 35.5 25.5 37.0 82-83 32.29720684216247 35.0 32.0 35.0 25.0 37.0 84-85 32.06304762809269 34.0 32.0 35.0 25.0 36.5 86-87 31.840676189852964 34.0 31.0 35.0 25.0 36.0 88-89 31.634948418165436 34.0 31.0 35.0 24.0 36.0 90-91 31.430804305219915 34.0 31.0 35.0 24.0 35.0 92-93 31.253512512236004 34.0 31.0 35.0 23.5 35.0 94-95 31.07828677233786 34.0 31.0 35.0 23.0 35.0 96-97 30.916400712762254 34.0 30.5 35.0 23.0 35.0 98-99 30.75116810275019 34.0 30.0 35.0 20.0 35.0 100 28.89913904481287 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3641460991452483 1101 2 -0.3315042835771784 1101 3 -0.3876240889010454 1101 4 -0.20300255426134584 1101 5 -0.23768444540286993 1101 6 -0.31008186430123885 1101 7 -0.3101125815018264 1101 8 -0.3174535538439329 1101 9 -0.44969954818015623 1101 10-11 -0.45163252148037003 1101 12-13 -0.43533556294242004 1101 14-15 -0.6662833387979958 1101 16-17 -0.6284980132032629 1101 18-19 -0.663564044942845 1101 20-21 -0.6752437938226947 1101 22-23 -0.6612621933250296 1101 24-25 -0.6912115928074272 1101 26-27 -0.7893411782788533 1101 28-29 -0.857077620567118 1101 30-31 -0.6662096446730601 1101 32-33 -0.6224780619822496 1101 34-35 -0.7273448298852756 1101 36-37 -0.6784209973124362 1101 38-39 -0.7236060037387517 1101 40-41 -0.7311443952477106 1101 42-43 -0.7113462164788302 1101 44-45 -0.7133883473718043 1101 46-47 -0.7063691654364703 1101 48-49 -0.7241496672006704 1101 50-51 -0.8349256014367725 1101 52-53 -0.7902661011101415 1101 54-55 -0.6672549438370652 1101 56-57 -0.6775700356531047 1101 58-59 -0.7099735515317462 1101 60-61 -0.7722621894683144 1101 62-63 -0.873697946979604 1101 64-65 -0.718740427723155 1101 66-67 -0.8514585223540081 1101 68-69 -0.876640480315551 1101 70-71 -1.0048496704283778 1101 72-73 -1.012686447993822 1101 74-75 -1.018531430631736 1101 76-77 -1.0050306048575095 1101 78-79 -0.8596279630831276 1101 80-81 -0.760022813281612 1101 82-83 -0.7459139671725268 1101 84-85 -0.6917578755429119 1101 86-87 -0.8561194557202612 1101 88-89 -0.8365191822919904 1101 90-91 -0.9428209479223888 1101 92-93 -0.9795123416135922 1101 94-95 -1.0037284145341694 1101 96-97 -1.0213315955667923 1101 98-99 -1.09501665118826 1101 100 -1.0761379406494562 1102 1 -0.38618092868289366 1102 2 -0.33016167858120227 1102 3 -0.39036661050883836 1102 4 -0.24728834332528038 1102 5 -0.28686394603345633 1102 6 -0.3657904619147416 1102 7 -0.3801217708533571 1102 8 -0.35125960999233286 1102 9 -0.4451040023874029 1102 10-11 -0.4994747749361039 1102 12-13 -0.5435175593044548 1102 14-15 -0.6374918177630207 1102 16-17 -0.7038339450082489 1102 18-19 -0.6983921839374361 1102 20-21 -0.7584819115713444 1102 22-23 -0.772944686048163 1102 24-25 -0.8125400853135858 1102 26-27 -0.8424358860434111 1102 28-29 -0.8189929945919872 1102 30-31 -0.7248634084730128 1102 32-33 -0.6715059632569833 1102 34-35 -0.7181125001472708 1102 36-37 -0.7988033525724987 1102 38-39 -0.8653017108421253 1102 40-41 -0.8374617817560903 1102 42-43 -0.7764617383156889 1102 44-45 -0.8731405118572013 1102 46-47 -0.8959768107091151 1102 48-49 -0.8976488112763619 1102 50-51 -0.9572825257796111 1102 52-53 -1.0016003993358495 1102 54-55 -0.9515387518838097 1102 56-57 -0.897259759551531 1102 58-59 -0.9808638420002112 1102 60-61 -0.9598453657866557 1102 62-63 -0.905317070500196 1102 64-65 -0.9309400223194686 1102 66-67 -0.9966653093048023 1102 68-69 -1.0975690392829236 1102 70-71 -1.1850250671001348 1102 72-73 -1.0520851608194235 1102 74-75 -1.1805631139775272 1102 76-77 -1.0051371115133136 1102 78-79 -0.8611861529245957 1102 80-81 -0.8347769947416168 1102 82-83 -0.8572400794405013 1102 84-85 -0.8773682889093273 1102 86-87 -0.9316790999596201 1102 88-89 -0.959391525722662 1102 90-91 -0.974872953654387 1102 92-93 -1.0450879367416945 1102 94-95 -1.1520502706217286 1102 96-97 -1.2227924701964525 1102 98-99 -1.2217360313259107 1102 100 -1.2214197260829387 1103 1 -0.40501412194016595 1103 2 -0.3905600576707151 1103 3 -0.42011270798867173 1103 4 -0.2606696351894513 1103 5 -0.264185585760373 1103 6 -0.3587669518307166 1103 7 -0.41752410723437805 1103 8 -0.3886556762539186 1103 9 -0.4967323068433984 1103 10-11 -0.520604551308061 1103 12-13 -0.550563123286075 1103 14-15 -0.7297919950229783 1103 16-17 -0.7234963018971143 1103 18-19 -0.7141760326131106 1103 20-21 -0.7080470071919649 1103 22-23 -0.7476261173346188 1103 24-25 -0.8210431483211664 1103 26-27 -0.7429680456161734 1103 28-29 -0.8532194558151218 1103 30-31 -0.7725374371566716 1103 32-33 -0.7634097941177131 1103 34-35 -0.8377773119289174 1103 36-37 -0.9148050586090832 1103 38-39 -0.8295104263254913 1103 40-41 -0.8559265241368479 1103 42-43 -0.7715839542253633 1103 44-45 -0.8857726925921625 1103 46-47 -0.9412455272000457 1103 48-49 -0.9495186183862216 1103 50-51 -0.967749324021888 1103 52-53 -0.9785445843815239 1103 54-55 -1.0007110028258808 1103 56-57 -0.9311050723638843 1103 58-59 -0.9919105449305832 1103 60-61 -0.9615652714805876 1103 62-63 -0.8346934120942251 1103 64-65 -0.9303283005720075 1103 66-67 -1.0328910979111754 1103 68-69 -1.0028541717709842 1103 70-71 -1.074868387012522 1103 72-73 -1.0070159002990877 1103 74-75 -1.0409193530913328 1103 76-77 -1.0672430404731656 1103 78-79 -0.8171257199079776 1103 80-81 -0.7414834483173713 1103 82-83 -0.8566761266738929 1103 84-85 -0.8781595606253667 1103 86-87 -0.8584002032680615 1103 88-89 -0.9418778454719394 1103 90-91 -0.8317181808112686 1103 92-93 -0.9871185129682765 1103 94-95 -1.062868637064259 1103 96-97 -1.1201179192921273 1103 98-99 -1.145436439508515 1103 100 -1.1816712802372642 1104 1 -0.3969400817721258 1104 2 -0.378947297605162 1104 3 -0.43131272859503156 1104 4 -0.27900167288388644 1104 5 -0.36278471227033293 1104 6 -0.388999774129168 1104 7 -0.3958075595257995 1104 8 -0.4258241640638616 1104 9 -0.6033123437574943 1104 10-11 -0.609397447265799 1104 12-13 -0.652947443704825 1104 14-15 -0.8201106271830056 1104 16-17 -0.7058247655705898 1104 18-19 -0.7753825032631951 1104 20-21 -0.8158088377967161 1104 22-23 -0.7708569579600564 1104 24-25 -0.7970030647302906 1104 26-27 -0.8914908272550619 1104 28-29 -0.9391906951651592 1104 30-31 -0.9454677754176544 1104 32-33 -0.8511679700495733 1104 34-35 -0.8527332109425529 1104 36-37 -0.8341508510481503 1104 38-39 -0.8567861516545534 1104 40-41 -0.9543464639983625 1104 42-43 -0.8628504472299738 1104 44-45 -0.9949678263667039 1104 46-47 -1.08969303017804 1104 48-49 -1.0427164825033302 1104 50-51 -1.0279962266285452 1104 52-53 -1.0019259189895209 1104 54-55 -1.050546405310719 1104 56-57 -0.9680103857220033 1104 58-59 -1.00103852857724 1104 60-61 -1.0139584488333568 1104 62-63 -1.0428127081337024 1104 64-65 -1.0646031889788574 1104 66-67 -1.1028954387589351 1104 68-69 -1.132598785931691 1104 70-71 -1.1492692296774791 1104 72-73 -1.0859615218911713 1104 74-75 -1.1189516442693161 1104 76-77 -1.091552452976579 1104 78-79 -0.9135012387089532 1104 80-81 -0.8814114886315174 1104 82-83 -0.9179542445363538 1104 84-85 -0.9616277501920472 1104 86-87 -1.0029429173366715 1104 88-89 -1.0343193020881607 1104 90-91 -1.023078869789945 1104 92-93 -1.0952216891767144 1104 94-95 -1.1019883317185553 1104 96-97 -1.0679148968290875 1104 98-99 -1.0690032841302362 1104 100 -1.2809186203067924 1105 1 -0.3972203204556273 1105 2 -0.36518961692092944 1105 3 -0.41715375625658524 1105 4 -0.26032962185995245 1105 5 -0.28357117630883266 1105 6 -0.37809433804798687 1105 7 -0.36753556427495937 1105 8 -0.4548948342811343 1105 9 -0.5665988579706536 1105 10-11 -0.5363598310586397 1105 12-13 -0.5376537318045607 1105 14-15 -0.7327326176276401 1105 16-17 -0.6700483311826773 1105 18-19 -0.6555426502168586 1105 20-21 -0.8063108273540465 1105 22-23 -0.7850373733639913 1105 24-25 -0.7290187234496415 1105 26-27 -0.8251781472204627 1105 28-29 -0.8209266433553282 1105 30-31 -0.7311806200251425 1105 32-33 -0.7740332495013007 1105 34-35 -0.793152491532993 1105 36-37 -0.7195871012369608 1105 38-39 -0.7374512669559223 1105 40-41 -0.8228941874947822 1105 42-43 -0.806268007093621 1105 44-45 -0.8088308738382679 1105 46-47 -0.8029050218163007 1105 48-49 -0.8778269671060457 1105 50-51 -0.879340696271818 1105 52-53 -0.9121487684243448 1105 54-55 -0.9107480757572048 1105 56-57 -0.901399509756935 1105 58-59 -0.9514089232035872 1105 60-61 -0.869661776099953 1105 62-63 -0.9239766651926473 1105 64-65 -0.9252825750100229 1105 66-67 -1.0037976319725743 1105 68-69 -0.9457638774661135 1105 70-71 -1.0121710362821617 1105 72-73 -0.9746729098490476 1105 74-75 -0.9697092288294655 1105 76-77 -0.914181541815303 1105 78-79 -0.8482283360889937 1105 80-81 -0.8169398489341724 1105 82-83 -0.8365437978551213 1105 84-85 -0.896136191682583 1105 86-87 -0.8858095911158159 1105 88-89 -0.9244827447247914 1105 90-91 -0.8843937311019054 1105 92-93 -1.0191664632481405 1105 94-95 -1.0216533656841555 1105 96-97 -1.0316297441977724 1105 98-99 -1.0266087063737679 1105 100 -1.0489187005862526 1106 1 -0.42872085723789866 1106 2 -0.4220066763712751 1106 3 -0.45263273166459683 1106 4 -0.3035520223451087 1106 5 -0.36206746182721616 1106 6 -0.4132765234495537 1106 7 -0.41518400749860973 1106 8 -0.42090672678589414 1106 9 -0.5344551091539387 1106 10-11 -0.5481379968267888 1106 12-13 -0.6204222557497516 1106 14-15 -0.7241553347189722 1106 16-17 -0.6993566702782132 1106 18-19 -0.6980720450132907 1106 20-21 -0.7172196676834375 1106 22-23 -0.7166195669980624 1106 24-25 -0.761307138941433 1106 26-27 -0.7454994429028474 1106 28-29 -0.7534235435090935 1106 30-31 -0.7555753081704211 1106 32-33 -0.7904247687831685 1106 34-35 -0.7813232282732585 1106 36-37 -0.7935544216864443 1106 38-39 -0.8072594864971236 1106 40-41 -0.869300617934158 1106 42-43 -0.8595834767783828 1106 44-45 -0.8488659578371767 1106 46-47 -0.851964809652884 1106 48-49 -0.8660393512113416 1106 50-51 -0.9411350522510773 1106 52-53 -0.9779236319282063 1106 54-55 -0.9143493120966824 1106 56-57 -0.900245247378102 1106 58-59 -0.9121998039715464 1106 60-61 -0.9763122891922578 1106 62-63 -1.004245625276802 1106 64-65 -0.9363791301964426 1106 66-67 -0.8817436658234215 1106 68-69 -0.9318686462887555 1106 70-71 -0.9847119641121722 1106 72-73 -0.9646453557799362 1106 74-75 -0.9985875440647831 1106 76-77 -0.9501444563722607 1106 78-79 -0.7822401928190388 1106 80-81 -0.7846116502414375 1106 82-83 -0.7774119476852057 1106 84-85 -0.7537697819365157 1106 86-87 -0.7927781974074861 1106 88-89 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1.1452889833895306 2206 86-87 1.117294833402589 2206 88-89 1.14056317361646 2206 90-91 1.1779152717221457 2206 92-93 1.165015524965682 2206 94-95 1.239338250715182 2206 96-97 1.309748204662462 2206 98-99 1.339048639282062 2206 100 1.6110860864142715 2207 1 0.705119069495403 2207 2 0.6349163774497768 2207 3 0.6321522325589015 2207 4 0.4054434551069548 2207 5 0.49407602998367395 2207 6 0.5124724300371142 2207 7 0.5136474347478881 2207 8 0.5291496032311542 2207 9 0.7283096731428671 2207 10-11 0.6813137376167404 2207 12-13 0.7618708425798744 2207 14-15 0.9406409127309416 2207 16-17 0.9414164476339764 2207 18-19 0.9134420935734013 2207 20-21 0.9194296554090968 2207 22-23 0.967345772872271 2207 24-25 0.9558334915887698 2207 26-27 1.0294731859618835 2207 28-29 0.9727002069613135 2207 30-31 1.03880585336249 2207 32-33 1.0783587192128152 2207 34-35 1.122353747766084 2207 36-37 1.1600652824523294 2207 38-39 1.1432797202927034 2207 40-41 1.132293331556781 2207 42-43 1.2183278771269812 2207 44-45 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2311 24-25 0.9775915404153963 2311 26-27 0.3332167552020735 2311 28-29 0.6693457735334576 2311 30-31 0.8885766621818476 2311 32-33 0.9822646483256392 2311 34-35 1.0495659963930066 2311 36-37 1.0475665370760012 2311 38-39 1.0666647593067964 2311 40-41 1.0954148186105073 2311 42-43 1.0292698840099845 2311 44-45 1.0513819146670755 2311 46-47 0.7643930076109058 2311 48-49 0.9765043537132456 2311 50-51 1.091608393536454 2311 52-53 1.0194679125822645 2311 54-55 1.0834117173654363 2311 56-57 1.1469787884475338 2311 58-59 1.143832554593537 2311 60-61 1.182863428825108 2311 62-63 1.198074739694455 2311 64-65 1.2372660095922257 2311 66-67 1.2374564447039091 2311 68-69 1.2643269527751855 2311 70-71 1.2671075684249615 2311 72-73 1.2949479293784307 2311 74-75 1.300146883872479 2311 76-77 1.2518545583520435 2311 78-79 0.9625629723868059 2311 80-81 0.9119353553611234 2311 82-83 1.026882990486051 2311 84-85 1.07825281174728 2311 86-87 1.0926600194299283 2311 88-89 1.1603063712819228 2311 90-91 1.2097484501702915 2311 92-93 1.2905981460098275 2311 94-95 1.2659052409513656 2311 96-97 1.2906664024335868 2311 98-99 1.3698644717117894 2311 100 1.5397876948825306 2312 1 0.29708466745339024 2312 2 0.3810149812684571 2312 3 0.4635372278081391 2312 4 0.22153777025129529 2312 5 0.33293992555289975 2312 6 0.43322542113170925 2312 7 0.2971006607252775 2312 8 0.41058550278030737 2312 9 0.5940940091478026 2312 10-11 0.6209453084784897 2312 12-13 0.7015146445258864 2312 14-15 0.904821458678839 2312 16-17 0.8692641393190925 2312 18-19 0.8327313705196957 2312 20-21 0.8430615384095503 2312 22-23 0.922894804943418 2312 24-25 0.9546270109719472 2312 26-27 1.0214575511806103 2312 28-29 0.9871885890046102 2312 30-31 0.9621782326272879 2312 32-33 0.8611190228362489 2312 34-35 0.9810106304561828 2312 36-37 1.0420241515538535 2312 38-39 0.830525346069031 2312 40-41 0.9960908042562835 2312 42-43 1.0528587754967802 2312 44-45 0.8188479836883502 2312 46-47 0.8408909849533757 2312 48-49 0.9799185843521059 2312 50-51 0.8700880316546176 2312 52-53 1.014255278121773 2312 54-55 0.9062189330896047 2312 56-57 0.9347300346743808 2312 58-59 1.0672618436644328 2312 60-61 1.0781355774587453 2312 62-63 1.0914438318386104 2312 64-65 1.1189007824713357 2312 66-67 1.1450816878428753 2312 68-69 1.146271288519209 2312 70-71 1.0936236257696734 2312 72-73 1.1387774788910932 2312 74-75 1.161915398648226 2312 76-77 1.1741402456600767 2312 78-79 1.0707240474194961 2312 80-81 0.9885152981843675 2312 82-83 0.9722011356127567 2312 84-85 1.041554332665033 2312 86-87 1.04350300106816 2312 88-89 1.0517602239206347 2312 90-91 1.1399920477518144 2312 92-93 1.1786550541531469 2312 94-95 1.2216078089495745 2312 96-97 0.8968677152762297 2312 98-99 0.6449602070498592 2312 100 0.8724589301334476 2313 1 0.05833571928025094 2313 2 0.21115480726817992 2313 3 0.288810493141213 2313 4 0.19643893267005552 2313 5 0.29969664742706925 2313 6 0.25189040695180864 2313 7 0.2407794940179997 2313 8 0.3807130204959748 2313 9 0.6145711148037449 2313 10-11 0.4000879078040427 2313 12-13 0.4538378600998172 2313 14-15 0.47307191451596964 2313 16-17 0.7310018766842319 2313 18-19 0.6960331865733167 2313 20-21 0.6981801553961517 2313 22-23 0.6788812518051515 2313 24-25 0.5135619214171214 2313 26-27 0.7542788232455635 2313 28-29 0.5880469015576111 2313 30-31 0.8281323131588891 2313 32-33 0.9248381676715312 2313 34-35 0.9563305242375364 2313 36-37 0.9632946578615176 2313 38-39 1.0050175737578613 2313 40-41 0.9939830312096589 2313 42-43 1.0268175227514078 2313 44-45 0.8670670351086613 2313 46-47 0.8588022549906071 2313 48-49 0.9946949419843136 2313 50-51 0.9408039220265607 2313 52-53 1.0346011379697657 2313 54-55 0.9564055697927429 2313 56-57 0.648683090022466 2313 58-59 0.6782964234668967 2313 60-61 0.6351382451154493 2313 62-63 0.9277953766807059 2313 64-65 1.0319533366499272 2313 66-67 1.0026119000826412 2313 68-69 1.0862081510770594 2313 70-71 1.0353778755447038 2313 72-73 1.0636700131597294 2313 74-75 1.1443805347159568 2313 76-77 1.1244141565219863 2313 78-79 0.9647117288337768 2313 80-81 0.840347964089446 2313 82-83 0.9276982002834728 2313 84-85 0.9170966681472983 2313 86-87 0.9481687575575961 2313 88-89 0.9649677117175237 2313 90-91 0.8266327563560729 2313 92-93 1.0199839729333107 2313 94-95 1.149985398500629 2313 96-97 1.2266493740816458 2313 98-99 1.2285688136374695 2313 100 1.329342845267604 2314 1 -0.34376095856107725 2314 2 0.06465770637133517 2314 3 0.29710327773050693 2314 4 0.22295353155069364 2314 5 0.31426686825569305 2314 6 0.3993932568900149 2314 7 0.4200265140345465 2314 8 0.45087918519639203 2314 9 0.4357735690628175 2314 10-11 0.5622860530217153 2314 12-13 0.44911049209414244 2314 14-15 0.7687160281928413 2314 16-17 0.7977530122909471 2314 18-19 0.8117467393264732 2314 20-21 0.8336417190173293 2314 22-23 0.8894886097448591 2314 24-25 0.8559471639548377 2314 26-27 0.7238990891568662 2314 28-29 0.7545856603716388 2314 30-31 0.8253914658611734 2314 32-33 0.8733112849574809 2314 34-35 0.8833590646890883 2314 36-37 0.893975660554041 2314 38-39 0.6534100723710168 2314 40-41 0.7029190473697184 2314 42-43 0.8858505014751259 2314 44-45 0.9191338437613368 2314 46-47 0.8627654239264189 2314 48-49 0.9133612943112084 2314 50-51 0.9376846921826854 2314 52-53 0.9063569595491643 2314 54-55 0.8143067575212157 2314 56-57 0.7924868499960169 2314 58-59 0.9041319832913146 2314 60-61 0.95105697762326 2314 62-63 0.9200510972152784 2314 64-65 0.970731332905693 2314 66-67 0.6949497991418596 2314 68-69 0.6463796044650891 2314 70-71 0.7681842553783085 2314 72-73 0.9204930273325616 2314 74-75 1.0116132400312097 2314 76-77 0.8772804089986224 2314 78-79 0.7954508804343021 2314 80-81 0.8592831144329978 2314 82-83 0.8803819303473546 2314 84-85 0.9111721632003515 2314 86-87 0.9327762084960582 2314 88-89 0.8959373462393039 2314 90-91 1.010389337721545 2314 92-93 0.8875619287513956 2314 94-95 0.9455546590331103 2314 96-97 0.9439921624866514 2314 98-99 1.050222055212025 2314 100 0.9707102351033114 2315 1 0.39404198389927814 2315 2 0.4294864625506847 2315 3 0.430754211646601 2315 4 0.315535161384922 2315 5 0.3789031712539028 2315 6 0.3919191650208731 2315 7 0.3965360422161126 2315 8 0.3026498916340472 2315 9 0.49936862441468577 2315 10-11 0.5666282340295581 2315 12-13 0.35879747066714884 2315 14-15 0.5917509110948984 2315 16-17 0.7535538975768858 2315 18-19 0.7674259589968528 2315 20-21 0.5007865028391123 2315 22-23 0.8269292889379471 2315 24-25 0.8742092499693257 2315 26-27 0.8312969525544247 2315 28-29 0.7697452059161876 2315 30-31 0.808544839094921 2315 32-33 0.7852908703368229 2315 34-35 0.8204315179332227 2315 36-37 0.8156648807278231 2315 38-39 0.8601724031606466 2315 40-41 0.8778769623882852 2315 42-43 0.9386972701381069 2315 44-45 0.96045779734024 2315 46-47 0.9319593159613788 2315 48-49 0.8324327331446923 2315 50-51 0.9190175146352928 2315 52-53 0.9315593510800468 2315 54-55 0.7447380074075909 2315 56-57 0.6980320684864623 2315 58-59 0.758107835119624 2315 60-61 0.9222487815054379 2315 62-63 0.9671848751802372 2315 64-65 1.0601027822468794 2315 66-67 1.0829686156194427 2315 68-69 1.1020502460122188 2315 70-71 0.9865134863735179 2315 72-73 1.0644126474956295 2315 74-75 0.42130039581104484 2315 76-77 0.453179800936347 2315 78-79 0.7186668884067728 2315 80-81 0.7677002157322335 2315 82-83 0.7240670533679463 2315 84-85 0.7790128809063823 2315 86-87 0.8496791870344111 2315 88-89 0.8185466374034469 2315 90-91 0.9215754795173332 2315 92-93 0.9450164852082779 2315 94-95 0.9778359941723771 2315 96-97 1.1080305171330451 2315 98-99 1.1088341759098306 2315 100 0.9795292874366233 2316 1 0.2703540239348996 2316 2 0.07250908776975962 2316 3 0.19710110943959336 2316 4 0.019547343642656756 2316 5 0.2725142584219 2316 6 0.36681921961118036 2316 7 0.4109568488186639 2316 8 0.4065309340215748 2316 9 0.6106284906889599 2316 10-11 0.5865184287589287 2316 12-13 0.4010926056652764 2316 14-15 0.510352782300771 2316 16-17 0.7534184607723446 2316 18-19 0.6827870951627233 2316 20-21 0.6660453976214242 2316 22-23 0.7109507474431496 2316 24-25 0.4520360106606063 2316 26-27 0.7031537194435558 2316 28-29 0.8799766426468452 2316 30-31 0.5332736961257893 2316 32-33 0.6546536837180099 2316 34-35 0.780068239713529 2316 36-37 0.8689763997441347 2316 38-39 0.7409937486885525 2316 40-41 0.7164127159602529 2316 42-43 0.7118828543467259 2316 44-45 0.6232444798196184 2316 46-47 0.7563434883128295 2316 48-49 0.8501851325015153 2316 50-51 0.8308664521252851 2316 52-53 0.8541570312998843 2316 54-55 0.8102000638100506 2316 56-57 0.8924282568620114 2316 58-59 0.7397065409599648 2316 60-61 0.834296532374573 2316 62-63 0.8563033443531296 2316 64-65 0.870937271263152 2316 66-67 0.8138768105273613 2316 68-69 0.861861891499494 2316 70-71 0.8712106474210728 2316 72-73 0.8759991064318129 2316 74-75 0.8769928782025929 2316 76-77 0.7413900200466585 2316 78-79 0.5191788328580529 2316 80-81 0.5273354175521803 2316 82-83 0.47580713219552706 2316 84-85 0.5424547368433927 2316 86-87 0.6003709036737774 2316 88-89 0.5833145914059834 2316 90-91 0.45433537093187937 2316 92-93 0.3323248162841175 2316 94-95 0.39634751431743354 2316 96-97 0.5887956561946694 2316 98-99 0.8065673121742094 2316 100 0.5061263104179119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 5.0 12 41.0 13 188.0 14 792.0 15 2308.0 16 5340.0 17 10766.0 18 18867.0 19 27937.0 20 41068.0 21 58994.0 22 73610.0 23 76406.0 24 77311.0 25 85132.0 26 100686.0 27 124409.0 28 154141.0 29 190684.0 30 236382.0 31 296594.0 32 378922.0 33 500824.0 34 721185.0 35 1015962.0 36 1607389.0 37 2361697.0 38 1892856.0 39 300288.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97930197266244 20.789219474870794 13.71793742774829 23.513541124718476 2 15.379895680051517 19.827469221059573 40.869639829596466 23.922995269292443 3 19.02874850229007 25.50173162003232 29.13349119731666 26.33602868036095 4 12.622387324491676 16.296560767738427 38.59528267766908 32.48576923010082 5 13.297019598259519 38.091152648371974 34.56042756469614 14.05140018867236 6 31.17874476706376 37.32492936368538 17.313073156965018 14.183252712285846 7 27.856189739434512 31.89441675711231 22.12583373669918 18.123559766754 8 26.50210630087316 35.76603700595841 19.25125989086962 18.480596802298816 9 26.15272524107625 15.642737923889413 21.083567276971348 37.12096955806299 10-11 25.986245329317413 26.232660376691058 26.847995827337762 20.933098466653764 12-13 25.851254097589702 23.58773075461765 27.806461971409302 22.754553176383347 14-15 23.160691054277915 25.00594789575754 25.382342321404117 26.451018728560427 16-17 22.143095403692033 27.899686066531192 26.510043535273454 23.447174994503317 18-19 21.683225204805257 27.59051939721362 28.764249116237732 21.962006281743392 20-21 22.97729995617141 26.704008959911175 28.428689552184927 21.89000153173249 22-23 23.172383686307395 26.47797210606344 28.118503770648225 22.231140436980944 24-25 22.641961338554182 26.424038736150752 28.783846090070025 22.15015383522504 26-27 22.591010252665114 26.723565271865183 27.998658786878718 22.686765688590988 28-29 22.002130337631296 27.046417945179712 28.456560155254433 22.49489156193456 30-31 22.794093730053607 26.678860639406103 28.424616645607685 22.102428984932605 32-33 22.93263452092926 26.880376303799597 27.795704522013793 22.391284653257348 34-35 22.908216730346552 26.808319156547576 28.351040497955747 21.932423615150118 36-37 22.65139261417826 26.47855148101704 28.108185058328367 22.761870846476338 38-39 22.720915467827226 26.623249901325902 28.49703210218336 22.158802528663518 40-41 22.505708356984435 25.972524794929143 28.908367755921976 22.61339909216445 42-43 22.539775119920634 26.10662910461353 28.36768280012057 22.98591297534527 44-45 22.578457699297473 26.798054601772826 28.208216376902374 22.415271322027326 46-47 22.58678016046646 26.67648595586629 27.663804785515783 23.072929098151462 48-49 23.16207053897553 26.62254049477134 27.69116763111228 22.524221335140847 50-51 22.858627610783174 26.510468616352608 27.644717938936346 22.986185833927873 52-53 22.44782686233348 26.921071168892098 27.4195499978643 23.211551970910126 54-55 22.400637933500104 26.84555890394708 27.44595270876798 23.307850453784837 56-57 23.111996952735698 26.533702591656205 27.153362224047378 23.20093823156072 58-59 22.320809819197247 26.90488693933774 27.719520812348158 23.05478242911686 60-61 22.494172021688804 27.313928424164086 27.222498274192574 22.969401279954536 62-63 22.75470119210437 26.79208004887715 26.948990750598174 23.504228008420306 64-65 22.160331338327108 26.894242913531073 27.213876144186937 23.73154960395488 66-67 22.469186546856452 26.653797527583933 27.227210773455024 23.649805152104587 68-69 22.170256344287647 26.654246041185175 27.41836415697793 23.757133457549244 70-71 22.12935215572119 26.339928585555185 27.503358657494964 24.02736060122866 72-73 22.544094565894795 26.469210522402488 27.562789939550242 23.423904972152478 74-75 22.633505679706094 26.761814416267182 27.233676577790007 23.371003326236718 76-77 22.41900622090693 26.673249984335012 26.95248261331193 23.955261181446126 78-79 22.330518598247448 26.657624762589684 27.05323059027384 23.958626048889027 80-81 22.00577210674754 27.05692773216761 27.33434172835135 23.6029584327335 82-83 22.696638793073813 26.292866221816947 28.089548503411688 22.920946481697552 84-85 22.84408942009277 26.581212255003354 27.159879544343568 23.4148187805603 86-87 21.8374100617987 27.11066680611959 27.513308144890814 23.5386149871909 88-89 22.44273757774444 26.2306931953468 27.430078058823543 23.896491168085223 90-91 22.318651325584188 26.231108253205406 27.48585932965577 23.96438109155464 92-93 22.515352694611416 26.409174162574367 27.342892987318006 23.732580155496212 94-95 21.67440068590673 26.35669678995728 27.85955515295925 24.109347371176742 96-97 21.871502476748976 26.33045657785446 27.53815432746617 24.2598866179304 98-99 21.401391717599548 26.6666808256393 27.478052707483574 24.453874749277578 100 21.87877537420768 26.227060279604466 26.99147866693018 24.902685679257676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 701.0 1 498.5 2 422.0 3 577.0 4 497.5 5 453.0 6 774.5 7 1536.0 8 2500.0 9 3097.0 10 3072.5 11 3052.5 12 3524.0 13 4263.5 14 5371.0 15 6604.0 16 7703.0 17 8790.5 18 9854.5 19 10886.5 20 12163.0 21 13519.0 22 15164.0 23 17613.5 24 20726.0 25 24683.5 26 30849.0 27 38359.5 28 48081.5 29 64707.0 30 87417.0 31 109786.5 32 139765.0 33 174021.0 34 202093.0 35 229804.5 36 257000.5 37 295983.5 38 317843.0 39 311997.0 40 300329.5 41 288402.0 42 291162.0 43 306151.0 44 329181.0 45 374572.5 46 417302.0 47 444999.0 48 468093.0 49 468039.0 50 452668.5 51 455931.5 52 455789.0 53 433114.5 54 411062.5 55 365617.0 56 318363.0 57 267003.0 58 217876.5 59 201741.0 60 166673.5 61 119611.0 62 90744.5 63 73358.0 64 53425.0 65 30612.0 66 19437.0 67 14143.0 68 10775.5 69 8041.5 70 6039.5 71 4296.0 72 3120.0 73 2436.5 74 1850.0 75 1299.0 76 856.0 77 514.5 78 296.0 79 169.5 80 108.0 81 60.5 82 36.5 83 26.0 84 23.0 85 19.5 86 13.0 87 8.0 88 6.0 89 5.0 90 4.0 91 3.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04499644719171787 2 0.0 3 0.0 4 0.032159622917804366 5 0.0 6 0.0 7 8.010950486727977E-4 8 0.0 9 0.010896823011464924 10-11 4.488062622082542E-4 12-13 1.4477621361556587E-5 14-15 1.0616922331808163E-4 16-17 2.316419417849054E-4 18-19 0.0 20-21 9.651747574371057E-6 22-23 0.0 24-25 5.501496117391503E-4 26-27 4.681097573569963E-4 28-29 1.8338320391305012E-4 30-31 0.0022681606799771986 32-33 0.014752696167426164 34-35 0.025369618499234324 36-37 0.03012793005339926 38-39 0.02146066073161405 40-41 0.0012643789322426087 42-43 6.756223302059741E-5 44-45 0.011495231361075931 46-47 0.018873992381682605 48-49 0.003624231214176333 50-51 0.011919908254348258 52-53 0.011586922963032455 54-55 0.010785827914359659 56-57 4.777615049313674E-4 58-59 0.00797716937021768 60-61 0.004343286408466976 62-63 0.0014284586410069167 64-65 0.03658977505444068 66-67 0.06110521389334317 68-69 0.06048750204858342 70-71 0.05298809418329711 72-73 0.046135353405493656 74-75 0.036367784860230144 76-77 0.03209206068478377 78-79 0.01585782126469165 80-81 0.0012499013108810521 82-83 0.03920539864709524 84-85 0.05281436272695843 86-87 0.05857645602885796 88-89 0.07088243418618105 90-91 0.04959550491090568 92-93 0.0402188321424042 94-95 0.013435232623524513 96-97 0.0034891067481351372 98-99 0.021726083789909253 100 0.03282559350043597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0360818E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.52466977971582 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1305730577531 44.86728454901605 2 15.04093103745999 18.206947684434887 3 5.144220223065622 9.340566908255484 4 2.2228862519550634 5.381578254298016 5 1.1691702364353622 3.538182123826129 6 0.6571702125885854 2.3865006063593865 7 0.40507779384293857 1.7162039795207762 8 0.25590833262678625 1.2391013860891131 9 0.18956609924621878 1.0326083002475606 >10 0.7318587176397744 7.3479006054578315 >50 0.033420501968197645 1.384408840650849 >100 0.017635152829289135 1.9404639044765304 >500 0.0011272587742255217 0.456077947422297 >1k 3.684335696336544E-4 0.32231699105615963 >5k 2.167256127487126E-5 0.07127911554879132 >10k+ 6.501768382461379E-5 0.7685788033401417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24624 0.23766463227131293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21221 0.20481973527572825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12748 0.12304047807808224 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.651747574371059E-6 0.0 10-11 0.0 0.0 0.0 9.651747574371059E-6 3.8606990297484237E-5 12-13 0.0 4.8258737871855296E-6 0.0 1.4477621361556589E-5 3.8606990297484237E-5 14-15 0.0 9.651747574371059E-6 0.0 2.8955242723113174E-5 4.825873787185529E-5 16-17 0.0 9.651747574371059E-6 0.0 4.825873787185529E-5 4.825873787185529E-5 18-19 0.0 9.651747574371059E-6 0.0 7.721398059496847E-5 4.825873787185529E-5 20-21 0.0 9.651747574371059E-6 0.0 1.0616922331808164E-4 4.825873787185529E-5 22-23 0.0 9.651747574371059E-6 0.0 2.654230582952041E-4 4.825873787185529E-5 24-25 0.0 9.651747574371059E-6 0.0 5.694531068878925E-4 4.825873787185529E-5 26-27 0.0 9.651747574371059E-6 0.0 0.0011919908254348257 4.825873787185529E-5 28-29 0.0 1.9303495148742118E-5 0.0 0.00424194305893608 4.825873787185529E-5 30-31 0.0 1.9303495148742118E-5 0.0 0.016325931022048646 4.825873787185529E-5 32-33 0.0 1.9303495148742118E-5 0.0 0.04396371020126017 6.756223302059741E-5 34-35 0.0 1.9303495148742118E-5 0.0 0.08545174714969417 6.756223302059741E-5 36-37 0.0 1.9303495148742118E-5 0.0 0.15570681774354111 6.756223302059741E-5 38-39 0.0 1.9303495148742118E-5 0.0 0.2573638490706043 6.756223302059741E-5 40-41 0.0 1.9303495148742118E-5 0.0 0.36276093258273623 6.756223302059741E-5 42-43 0.0 1.9303495148742118E-5 0.0 0.4674872196384494 6.756223302059741E-5 44-45 0.0 1.9303495148742118E-5 0.0 0.5895383935901586 6.756223302059741E-5 46-47 0.0 1.9303495148742118E-5 0.0 0.7360229665263882 6.756223302059741E-5 48-49 0.0 1.9303495148742118E-5 0.0 0.8964494888337967 6.756223302059741E-5 50-51 0.0 1.9303495148742118E-5 0.0 1.06129168565648 6.756223302059741E-5 52-53 0.0 2.4129368935927644E-5 0.0 1.218813031944003 6.756223302059741E-5 54-55 0.0 3.860699029748423E-5 0.0 1.3890457297869725 6.756223302059741E-5 56-57 0.0 4.825873787185529E-5 0.0 1.591790339334211 9.169160195652504E-5 58-59 0.0 5.791048544622635E-5 0.0 1.819914219128258 1.0616922331808164E-4 60-61 0.0 5.791048544622635E-5 0.0 2.0513534742141015 1.0616922331808164E-4 62-63 0.0 5.791048544622635E-5 0.0 2.284974024251753 1.0616922331808164E-4 64-65 0.0 6.756223302059741E-5 0.0 2.5235893536591414 1.0616922331808164E-4 66-67 0.0 6.756223302059741E-5 0.0 2.7896590790418285 1.0616922331808164E-4 68-69 0.0 6.756223302059741E-5 0.0 3.083284543749345 1.0616922331808164E-4 70-71 0.0 6.756223302059741E-5 0.0 3.398457534916645 1.0616922331808164E-4 72-73 0.0 6.756223302059741E-5 0.0 3.7155946567153286 1.0616922331808164E-4 74-75 0.0 6.756223302059741E-5 0.0 4.023162070794024 1.1099509710526718E-4 76-77 0.0 6.756223302059741E-5 0.0 4.36368537696541 1.158209708924527E-4 78-79 0.0 6.756223302059741E-5 0.0 4.736735072462425 1.158209708924527E-4 80-81 0.0 6.756223302059741E-5 0.0 5.134420853643023 1.2547271846682375E-4 82-83 0.0 6.756223302059741E-5 0.0 5.5343796213773855 1.2547271846682375E-4 84-85 9.651747574371059E-6 6.756223302059741E-5 0.0 5.926312961003658 1.3512446604119481E-4 86-87 9.651747574371059E-6 6.756223302059741E-5 0.0 6.335296112720058 1.3512446604119481E-4 88 1.9303495148742118E-5 6.756223302059741E-5 0.0 6.667031502724978 1.3512446604119481E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17655 0.0 45.353127 1 GTATCAA 30095 0.0 37.932777 1 CAACGCA 33925 0.0 32.022377 5 ATCAACG 34540 0.0 31.43471 3 GGGCACC 9765 0.0 29.992682 94 TATCAAC 38005 0.0 29.937475 2 AACGCAG 38775 0.0 29.03492 6 GTGGTAT 6535 0.0 28.994776 1 CTGTGCG 11505 0.0 28.425097 9 TAGTACT 12025 0.0 27.869184 4 TCAACGC 40455 0.0 27.30332 4 TAGGCAT 10925 0.0 27.225 5 ACGCAGA 41830 0.0 25.937117 7 CTAGGCA 11970 0.0 25.876831 4 GCATCGT 10395 0.0 25.67457 8 CTAGTAC 13375 0.0 25.61849 3 CGCAGAG 42625 0.0 25.419926 8 GTACTGT 13825 0.0 25.252787 6 GTACATG 42750 0.0 24.614119 1 TACCTGG 5695 0.0 24.591421 2 >>END_MODULE