FastQCFastQC Report
Mon 6 Jun 2016
ERR1211200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221640.2777217742567052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171330.21468178841094251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.13982572094074053No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117750.045.5224761
GTATCAA203800.040.5493051
ATCAACG234700.033.7859883
CAACGCA234550.033.6071935
TATCAAC254200.032.1755832
AACGCAG258300.031.1358156
TCAACGC258400.030.7235774
TACCTGG58900.030.4412062
GTACTAG46250.028.6946161
GTGGTAT50200.028.2179741
ACGCAGA287350.027.4815277
GTACATG255050.026.9211141
GTACCTG67850.026.8425751
CGCAGAG294850.026.7665488
TACATGG255050.026.3115462
GCATCGT42600.026.1505938
CTGTGCG57400.026.1234029
ACATGGG260550.024.661083
TAGTACT63650.024.5912424
GCAGAGT322850.023.9360229
Produced by FastQC (version 0.11.5)