FastQCFastQC Report
Mon 6 Jun 2016
ERR1211200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204740.2565455516211777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167470.20984508903975105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108250.13564059765064218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118150.046.9531941
GTATCAA206250.039.2742771
ATCAACG244150.031.994613
CAACGCA246450.031.6676185
TATCAAC265200.030.3358762
GTACTAG44650.029.5870551
AACGCAG272100.029.2522626
TCAACGC270650.029.105064
TACCTGG64550.028.9015122
GTACCTG74050.025.7761381
ACGCAGA303500.025.7459057
GTACATG263750.025.5963971
GTGGTAT53750.025.4525571
CGCAGAG309900.025.1837238
TACATGG266250.025.1331372
CTGTGCG62050.024.8372739
TAGTACT67750.023.7257164
ACATGGG265050.023.7086123
ACCTGGG79100.023.2922843
CTAGTAC70000.023.2316783
Produced by FastQC (version 0.11.5)