FastQCFastQC Report
Mon 6 Jun 2016
ERR1211199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236488
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414950.3692879839323461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312470.2780851098670688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212450.18907153195909612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218800.044.1346171
GTATCAA385550.039.9986231
GTACTAG121150.038.144981
GGGCACC126100.036.747194
GCATCGT118000.036.1717458
TACCTGG61250.035.4963152
CAACGCA439250.033.2086455
ATCAACG445100.032.83543
ACCTGGG64200.032.510763
CTGTGCG162150.032.2656179
TAGGCAT151750.031.907275
GTGGTAT63850.031.766231
TAGTACT171000.031.311714
TATCAAC489350.031.2832472
ACTGTGC175700.031.0885188
GTACATG583750.030.8760131
TACATGG591900.030.053212
CTAGGCA168550.029.953994
AACGCAG506400.029.68756
CTAACGC56550.029.593523
Produced by FastQC (version 0.11.5)