##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211199_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11236488 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53962448053164 31.0 31.0 34.0 30.0 34.0 2 31.557413490763306 31.0 31.0 34.0 29.0 34.0 3 31.622046675082107 33.0 31.0 34.0 28.0 34.0 4 35.22273525322147 37.0 35.0 37.0 32.0 37.0 5 35.28745298353009 37.0 35.0 37.0 33.0 37.0 6 35.348472138269535 37.0 35.0 37.0 32.0 37.0 7 35.26774807217344 37.0 35.0 37.0 32.0 37.0 8 35.27878114585269 37.0 35.0 37.0 32.0 37.0 9 36.84742910774256 39.0 37.0 39.0 33.0 39.0 10-11 36.80267433205108 39.0 37.0 39.0 32.0 39.0 12-13 36.67794425624804 39.0 37.0 39.0 32.0 39.0 14-15 37.861711817785064 40.0 37.5 41.0 32.0 41.0 16-17 37.847154199782 40.0 37.5 41.0 32.0 41.0 18-19 37.868620960570595 40.0 37.5 41.0 32.0 41.0 20-21 37.83952810700283 40.0 37.5 41.0 32.0 41.0 22-23 37.72352807211648 40.0 37.0 41.0 32.0 41.0 24-25 37.62039869574906 40.0 37.0 41.0 32.0 41.0 26-27 37.4675497806788 40.0 37.0 41.0 31.5 41.0 28-29 37.35415104790749 40.0 36.0 41.0 31.0 41.0 30-31 37.196596970512495 39.5 36.0 41.0 31.0 41.0 32-33 37.01329725978437 39.0 36.0 41.0 30.5 41.0 34-35 36.866336972904705 39.0 35.0 41.0 30.0 41.0 36-37 36.759446501433544 39.0 35.0 41.0 30.0 41.0 38-39 36.60454147238888 39.0 35.0 41.0 30.0 41.0 40-41 36.4312499599519 39.0 35.0 40.5 29.5 41.0 42-43 36.2129474974743 39.0 35.0 40.0 29.0 41.0 44-45 35.95611404559859 38.0 35.0 40.0 28.0 41.0 46-47 35.68550738451374 38.0 34.5 40.0 27.0 41.0 48-49 35.43998520712166 38.0 34.0 40.0 26.0 41.0 50-51 34.73107558162302 37.5 33.5 39.5 25.5 40.5 52-53 34.81350801068804 37.5 33.5 39.5 26.0 40.5 54-55 35.26068612363578 38.0 34.0 40.0 26.5 41.0 56-57 35.1823272538537 38.0 34.0 40.0 26.0 41.0 58-59 34.934710160327676 37.0 34.0 40.0 26.0 41.0 60-61 34.70464659420274 37.0 34.0 40.0 26.0 41.0 62-63 34.38561879832916 36.0 33.0 40.0 26.0 41.0 64-65 34.131708724291784 36.0 33.0 39.0 26.0 41.0 66-67 33.9678509423941 35.0 33.0 39.0 25.5 41.0 68-69 33.684597803157004 35.0 33.0 39.0 25.0 41.0 70-71 33.42917213100748 35.0 33.0 38.0 25.0 40.5 72-73 33.13591235090537 35.0 32.5 37.0 24.0 40.0 74-75 32.82774742428417 35.0 32.0 37.0 24.0 39.5 76-77 32.53519088882576 35.0 32.0 36.0 23.0 39.0 78-79 32.22267802893573 35.0 32.0 36.0 23.0 39.0 80-81 31.956261823089207 35.0 31.0 35.5 22.5 38.0 82-83 31.67640783312366 35.0 31.0 35.0 21.0 37.0 84-85 31.456083787033812 35.0 31.0 35.0 20.0 37.0 86-87 31.24310927934066 34.0 31.0 35.0 20.0 36.0 88-89 31.052635618887322 34.0 31.0 35.0 20.0 36.0 90-91 30.82573914554085 34.0 30.5 35.0 19.0 36.0 92-93 30.62684910979302 34.0 30.0 35.0 19.0 35.5 94-95 30.46934326811011 34.0 30.0 35.0 18.0 35.0 96-97 30.28443478068948 34.0 29.5 35.0 18.0 35.0 98-99 30.056770896742826 34.0 29.0 35.0 17.0 35.0 100 28.890303981101567 33.0 27.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.48631917350282095 1101 2 -0.47086492639814637 1101 3 -0.4946742876691985 1101 4 -0.3993657126233572 1101 5 -0.35291432911586895 1101 6 -0.3207508706543152 1101 7 -0.35843415174129944 1101 8 -0.32895829940529353 1101 9 -0.5654572472905954 1101 10-11 -0.5416564788661304 1101 12-13 -0.5675237508676645 1101 14-15 -0.7544182956138954 1101 16-17 -0.7647067320336305 1101 18-19 -0.6647579370198216 1101 20-21 -0.757805550862372 1101 22-23 -0.8064178751969635 1101 24-25 -0.8761458426000246 1101 26-27 -0.8257523164109486 1101 28-29 -0.7992900830784464 1101 30-31 -0.8506509183789319 1101 32-33 -0.8723790848253117 1101 34-35 -0.8569031887795688 1101 36-37 -0.9391309444750959 1101 38-39 -0.9285476747978976 1101 40-41 -0.957971414053965 1101 42-43 -1.0538952685325356 1101 44-45 -1.0182170444948895 1101 46-47 -1.1260987993143843 1101 48-49 -1.1588549461333884 1101 50-51 -1.1199936131864163 1101 52-53 -0.9850464348228911 1101 54-55 -0.918930694887024 1101 56-57 -0.8460168486722779 1101 58-59 -0.7907344493528328 1101 60-61 -0.7633951088808786 1101 62-63 -0.8240151337353154 1101 64-65 -0.7895037599893939 1101 66-67 -0.8352349438887643 1101 68-69 -0.7593024288616093 1101 70-71 -0.82380907143515 1101 72-73 -0.880860963450111 1101 74-75 -0.8993467655371994 1101 76-77 -0.8905743207085735 1101 78-79 -0.9364152122733174 1101 80-81 -1.0132456225671582 1101 82-83 -0.9890482440592194 1101 84-85 -1.044474039060237 1101 86-87 -1.11741300089162 1101 88-89 -1.1512721571616495 1101 90-91 -1.2110036001231848 1101 92-93 -1.3415387715097964 1101 94-95 -1.3727957091918483 1101 96-97 -1.4092089525361189 1101 98-99 -1.483532192598961 1101 100 -1.5758677767749134 1102 1 -0.4045097322818094 1102 2 -0.3640004996857584 1102 3 -0.38298540807869585 1102 4 -0.19272311360296612 1102 5 -0.21890695898317603 1102 6 -0.2683664586811716 1102 7 -0.24433567043917037 1102 8 -0.359752060300778 1102 9 -0.38842412195629095 1102 10-11 -0.41707353658744495 1102 12-13 -0.49238307949296 1102 14-15 -0.6081941953666288 1102 16-17 -0.6395296330097935 1102 18-19 -0.6640749727495034 1102 20-21 -0.684252431857999 1102 22-23 -0.6744423753711359 1102 24-25 -0.6583900966766976 1102 26-27 -0.6724557479490088 1102 28-29 -0.7889658069234855 1102 30-31 -0.787633124001097 1102 32-33 -0.7601427074792113 1102 34-35 -0.7637533451292313 1102 36-37 -0.783211355930419 1102 38-39 -0.9372056057940696 1102 40-41 -0.9172222913627408 1102 42-43 -0.8203996692830557 1102 44-45 -1.0224236671154046 1102 46-47 -0.9956816740636683 1102 48-49 -0.968904925530552 1102 50-51 -1.0379527882269457 1102 52-53 -0.9093428740870877 1102 54-55 -0.74427949695378 1102 56-57 -0.7948411109391245 1102 58-59 -0.775352246992469 1102 60-61 -0.684297214957077 1102 62-63 -0.7992658652825213 1102 64-65 -0.7743644341060403 1102 66-67 -0.784354118490981 1102 68-69 -0.8192253128407572 1102 70-71 -0.8613249886232168 1102 72-73 -0.8979006429581489 1102 74-75 -0.8915178406841662 1102 76-77 -0.9439638249077547 1102 78-79 -0.9414486495169783 1102 80-81 -0.978611834476439 1102 82-83 -1.0516955441448204 1102 84-85 -0.921120845289348 1102 86-87 -1.010514248860325 1102 88-89 -1.0407706991637866 1102 90-91 -1.12379952858484 1102 92-93 -1.1499120597351933 1102 94-95 -1.1667856949648048 1102 96-97 -1.270152345829608 1102 98-99 -1.33697003765187 1102 100 -1.3627878171294583 1103 1 -0.4470982986888714 1103 2 -0.4224245637590869 1103 3 -0.5139883196561215 1103 4 -0.392414650748222 1103 5 -0.35815088011484164 1103 6 -0.4015155520969529 1103 7 -0.37847516683482496 1103 8 -0.390343732417719 1103 9 -0.4971922978245331 1103 10-11 -0.5155515361205829 1103 12-13 -0.6059660267019353 1103 14-15 -0.6953450268665193 1103 16-17 -0.739445583994268 1103 18-19 -0.6284076785209507 1103 20-21 -0.7210740568827276 1103 22-23 -0.7619253862232185 1103 24-25 -0.7732118432772594 1103 26-27 -0.7683634829452188 1103 28-29 -0.8177735583856389 1103 30-31 -0.8702209269774315 1103 32-33 -0.8906183299387678 1103 34-35 -0.9498720254501904 1103 36-37 -0.8363497469889083 1103 38-39 -0.8373596478988077 1103 40-41 -0.8508146862827957 1103 42-43 -0.9959184874922968 1103 44-45 -1.0035004247534687 1103 46-47 -1.0157056382155716 1103 48-49 -1.089182234066783 1103 50-51 -1.1351730407546796 1103 52-53 -0.9523139694331348 1103 54-55 -0.7303094156606988 1103 56-57 -0.7095597329896393 1103 58-59 -0.7932818371887933 1103 60-61 -0.768367158310717 1103 62-63 -0.7433518764992186 1103 64-65 -0.7048495278140905 1103 66-67 -0.7929199238147078 1103 68-69 -0.7527892991321607 1103 70-71 -0.6749282854568506 1103 72-73 -0.7592762059499378 1103 74-75 -0.7331582025912624 1103 76-77 -0.7699738125465245 1103 78-79 -0.882569208225469 1103 80-81 -0.9169171860951337 1103 82-83 -0.8393958769126044 1103 84-85 -0.9713613771365388 1103 86-87 -1.0382387678503555 1103 88-89 -1.0390307013599305 1103 90-91 -1.1135131642865446 1103 92-93 -1.192467494681427 1103 94-95 -1.1489826541567716 1103 96-97 -1.1878306844242346 1103 98-99 -1.2682861553835316 1103 100 -1.3119463087254282 1104 1 -0.5399258719251101 1104 2 -0.5390764259361767 1104 3 -0.5777994782220937 1104 4 -0.416396445034529 1104 5 -0.41937530458930183 1104 6 -0.48209425367920034 1104 7 -0.5165017299948502 1104 8 -0.4722280165728421 1104 9 -0.5827462438206439 1104 10-11 -0.6336111111855445 1104 12-13 -0.6166176422185572 1104 14-15 -0.8099324953482281 1104 16-17 -0.8562190949944437 1104 18-19 -0.8450766201522839 1104 20-21 -0.7940314039894929 1104 22-23 -0.8217785924567238 1104 24-25 -0.8280299226787449 1104 26-27 -0.8497922879765767 1104 28-29 -0.8785510627084818 1104 30-31 -0.867126114067041 1104 32-33 -0.8818061337620193 1104 34-35 -0.9944295786529622 1104 36-37 -1.0167901700197035 1104 38-39 -0.9783967104968454 1104 40-41 -0.9184050514042923 1104 42-43 -1.0229608284135097 1104 44-45 -1.0078628163746046 1104 46-47 -1.0817654464087028 1104 48-49 -1.0864841624346084 1104 50-51 -1.128372709480928 1104 52-53 -1.0344918111395174 1104 54-55 -0.8415576492711665 1104 56-57 -0.8764979368460857 1104 58-59 -0.8565918248873672 1104 60-61 -0.8893474294443706 1104 62-63 -0.8539803696234003 1104 64-65 -0.788049867887878 1104 66-67 -0.9223027860662683 1104 68-69 -0.9736882164758711 1104 70-71 -0.9116903815230017 1104 72-73 -0.8657789301137697 1104 74-75 -0.9258189234767187 1104 76-77 -0.880385181260074 1104 78-79 -0.8936384104222981 1104 80-81 -0.9175070360171489 1104 82-83 -0.9462215630018385 1104 84-85 -0.9284775233407174 1104 86-87 -0.9229756976617587 1104 88-89 -1.0073919695577231 1104 90-91 -1.0900072488517019 1104 92-93 -1.0799873869481793 1104 94-95 -1.0655694944808296 1104 96-97 -1.1296694907593903 1104 98-99 -1.1450421019548038 1104 100 -1.1025685275659285 1105 1 -0.5728499479835847 1105 2 -0.5816580563070133 1105 3 -0.5822025675761182 1105 4 -0.4315039441699753 1105 5 -0.38197739609515935 1105 6 -0.35116383022029396 1105 7 -0.4027802574354453 1105 8 -0.43529357802628255 1105 9 -0.5695303297889112 1105 10-11 -0.6102492697156663 1105 12-13 -0.606352020098555 1105 14-15 -0.8113554531550804 1105 16-17 -0.7474774513408846 1105 18-19 -0.8041620237051674 1105 20-21 -0.7552951201376032 1105 22-23 -0.8001843772316448 1105 24-25 -0.804961838830117 1105 26-27 -0.770865808766203 1105 28-29 -0.8511864267044018 1105 30-31 -0.8734988978762601 1105 32-33 -0.9308760360708774 1105 34-35 -0.8839729194797243 1105 36-37 -0.8628105729928848 1105 38-39 -0.9054165205573312 1105 40-41 -0.9591766058397369 1105 42-43 -0.9637527490964359 1105 44-45 -1.1130497978497118 1105 46-47 -1.1436203887858412 1105 48-49 -1.135021772514726 1105 50-51 -1.1257873024321938 1105 52-53 -0.9847435973495067 1105 54-55 -0.7523861869419406 1105 56-57 -0.82458183080346 1105 58-59 -0.770501038406394 1105 60-61 -0.8207485181694452 1105 62-63 -0.8748806572594319 1105 64-65 -0.8289504328874955 1105 66-67 -0.8002549027384589 1105 68-69 -0.7722934483368462 1105 70-71 -0.7403423975529222 1105 72-73 -0.7567213724054298 1105 74-75 -0.808782744738707 1105 76-77 -0.7550824150845727 1105 78-79 -0.7952071339359037 1105 80-81 -0.8479624954584111 1105 82-83 -0.9715817092500529 1105 84-85 -0.8603283674979529 1105 86-87 -0.9245217211553047 1105 88-89 -0.9810636438280689 1105 90-91 -1.0597040282148065 1105 92-93 -1.0217203846177867 1105 94-95 -1.0906273451496595 1105 96-97 -1.0902869631903336 1105 98-99 -0.9788877319838889 1105 100 -1.0187343455076956 1106 1 -0.5550554612383429 1106 2 -0.5199607696037241 1106 3 -0.6202773814282345 1106 4 -0.4575773010348385 1106 5 -0.449515247998562 1106 6 -0.48586427471190063 1106 7 -0.5160437285004988 1106 8 -0.4513545691873375 1106 9 -0.577566053526489 1106 10-11 -0.576070049307674 1106 12-13 -0.6499286025231186 1106 14-15 -0.8509677067019439 1106 16-17 -0.8339383349650547 1106 18-19 -0.9284309924739915 1106 20-21 -0.7358831537648811 1106 22-23 -0.7912446643527602 1106 24-25 -0.7911312221083691 1106 26-27 -0.8768374004810298 1106 28-29 -0.9452224742169193 1106 30-31 -0.9405937329928875 1106 32-33 -0.9307370217850135 1106 34-35 -0.9091628151355309 1106 36-37 -0.9698375896427649 1106 38-39 -0.9862042605142136 1106 40-41 -1.0083068768857544 1106 42-43 -1.0047970541863975 1106 44-45 -0.9728231311007249 1106 46-47 -0.9369682439742704 1106 48-49 -0.9824414943949407 1106 50-51 -1.0098183527906315 1106 52-53 -0.9377210059081591 1106 54-55 -0.8187533997897276 1106 56-57 -0.7784512519450573 1106 58-59 -0.7751421308939612 1106 60-61 -0.7605683100847216 1106 62-63 -0.760860350207544 1106 64-65 -0.7569134505730801 1106 66-67 -0.8281566267714453 1106 68-69 -0.8607652813904494 1106 70-71 -0.8243266178996507 1106 72-73 -0.830441571006439 1106 74-75 -0.7163370442017154 1106 76-77 -0.7320272621619992 1106 78-79 -0.7561270539391671 1106 80-81 -0.6843520527449307 1106 82-83 -0.7877222944673328 1106 84-85 -0.7878313861561175 1106 86-87 -0.7962811235554454 1106 88-89 -0.7772339066232377 1106 90-91 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54-55 1.135988585818282 2311 56-57 1.0873526346714613 2311 58-59 1.0339889606967105 2311 60-61 1.0276888964632391 2311 62-63 1.0248676730154358 2311 64-65 1.0433262850796723 2311 66-67 1.0250049750828296 2311 68-69 1.0977072160844301 2311 70-71 1.0820144506466889 2311 72-73 1.0613123667015643 2311 74-75 1.073305799736488 2311 76-77 1.1204684000525305 2311 78-79 1.1774399292230626 2311 80-81 1.172931745908528 2311 82-83 1.2400907058340138 2311 84-85 1.2069396150895138 2311 86-87 1.2125527769701314 2311 88-89 1.2503086017112288 2311 90-91 1.2780318147535539 2311 92-93 1.35576309340318 2311 94-95 1.4002240762461895 2311 96-97 1.413079599273665 2311 98-99 1.4553837847234057 2311 100 1.5521322263066004 2312 1 0.5905600826367952 2312 2 0.6125119006482471 2312 3 0.6398206123117696 2312 4 0.33026442262526956 2312 5 0.42056113136026596 2312 6 0.5159288456981486 2312 7 0.40703862271904256 2312 8 0.5026338235641816 2312 9 0.6915335929365796 2312 10-11 0.6395616759787259 2312 12-13 0.7927215532024405 2312 14-15 0.9914819071419458 2312 16-17 1.0480316286825087 2312 18-19 1.0052070958896806 2312 20-21 1.0092596522966488 2312 22-23 1.0272622289364435 2312 24-25 1.0391286912258053 2312 26-27 1.1041805850661248 2312 28-29 1.1300335055882869 2312 30-31 1.1523565846402448 2312 32-33 1.1395795485195421 2312 34-35 1.1824308622707633 2312 36-37 1.202582764406884 2312 38-39 1.1771583286077245 2312 40-41 1.2418275120112767 2312 42-43 1.2704237294804557 2312 44-45 1.3133424031742464 2312 46-47 1.3560805988124756 2312 48-49 1.3596923756394474 2312 50-51 1.3201654390939055 2312 52-53 1.1747702117473793 2312 54-55 1.123547832155225 2312 56-57 1.0389423431588867 2312 58-59 1.032385229563907 2312 60-61 1.014570091118678 2312 62-63 0.9816734886897436 2312 64-65 1.0258077757159327 2312 66-67 0.9667035954235388 2312 68-69 0.9625045400038417 2312 70-71 0.9748967037345793 2312 72-73 0.9680477522530069 2312 74-75 1.0125025794399392 2312 76-77 1.024471772881661 2312 78-79 1.0391582657728904 2312 80-81 1.03527555582167 2312 82-83 1.0604261296242328 2312 84-85 0.9982882502715178 2312 86-87 1.052685350386998 2312 88-89 1.0032144582651163 2312 90-91 1.1416518702525948 2312 92-93 1.191905339540483 2312 94-95 1.2087061922237652 2312 96-97 1.2236478676380464 2312 98-99 0.8705706265701529 2312 100 1.1677341754279027 2313 1 0.6451048034308933 2313 2 0.6348751188549322 2313 3 0.6707411259717482 2313 4 0.5735784840073421 2313 5 0.3871900289107941 2313 6 0.5600516574598728 2313 7 0.609157305681272 2313 8 0.594575302007982 2313 9 0.7157411605490793 2313 10-11 0.754709778529616 2313 12-13 0.8246412901493869 2313 14-15 0.9917077030147752 2313 16-17 1.0172376198868278 2313 18-19 0.9768665142439161 2313 20-21 1.0251421023391032 2313 22-23 1.0516673464349324 2313 24-25 1.0450935314746275 2313 26-27 1.0597749005836405 2313 28-29 1.1492900092869647 2313 30-31 1.1337208389688982 2313 32-33 1.1388849587166376 2313 34-35 1.1583061403784782 2313 36-37 1.2048324316822345 2313 38-39 1.2336601247221992 2313 40-41 1.268129824026154 2313 42-43 1.2612797019206354 2313 44-45 1.2538545486733526 2313 46-47 1.3108875015399093 2313 48-49 1.2671215896826524 2313 50-51 1.3140078311663643 2313 52-53 1.196019502501315 2313 54-55 1.059755285820458 2313 56-57 0.9874222480486949 2313 58-59 1.0098043865219992 2313 60-61 0.9774561717853629 2313 62-63 0.9881288148232059 2313 64-65 1.033987225188227 2313 66-67 0.9970152377656234 2313 68-69 1.030326921669669 2313 70-71 1.0148936403695714 2313 72-73 1.0050879258216696 2313 74-75 1.0247538203711173 2313 76-77 0.8816896684104591 2313 78-79 1.0178744846391723 2313 80-81 1.0805041756050926 2313 82-83 1.127564707256802 2313 84-85 1.1348874596040766 2313 86-87 1.1514950245927587 2313 88-89 1.15787697617775 2313 90-91 1.2713618971581866 2313 92-93 1.3322527328384304 2313 94-95 1.3666853256988176 2313 96-97 1.3919980432861117 2313 98-99 1.4356086282598177 2313 100 1.5736197858556729 2314 1 0.5346291150920344 2314 2 0.5835415887405766 2314 3 0.5752387413540099 2314 4 0.45725046698474614 2314 5 0.4941101268030721 2314 6 0.48988905744613476 2314 7 0.5569902751991691 2314 8 0.5349079263942684 2314 9 0.6852016478547043 2314 10-11 0.6732377057278498 2314 12-13 0.7523863484846913 2314 14-15 0.9153434388411128 2314 16-17 0.912815092142317 2314 18-19 0.8553657568664406 2314 20-21 0.9366037198517603 2314 22-23 0.9607558460485066 2314 24-25 0.9587245040202887 2314 26-27 1.034005106841498 2314 28-29 0.9753744040878161 2314 30-31 0.9766724837757721 2314 32-33 0.974727476757586 2314 34-35 1.0190926793549124 2314 36-37 1.0680492763341078 2314 38-39 1.0705629220556219 2314 40-41 1.0993094732413127 2314 42-43 1.175687705455914 2314 44-45 1.193615699565882 2314 46-47 1.207281683311919 2314 48-49 1.2228192606137682 2314 50-51 1.2142962093242033 2314 52-53 1.1429642887084626 2314 54-55 1.0531205258684437 2314 56-57 1.033227753011353 2314 58-59 1.0287121442449063 2314 60-61 0.9771891816810694 2314 62-63 0.964110208254624 2314 64-65 0.9739510763730408 2314 66-67 0.9262726254260372 2314 68-69 1.0137106412144377 2314 70-71 0.972524908796963 2314 72-73 0.9937566115424445 2314 74-75 0.9940901914089935 2314 76-77 0.9837847880499808 2314 78-79 0.9832011337550668 2314 80-81 1.0189620182765395 2314 82-83 1.0720877156944013 2314 84-85 1.0952349070313865 2314 86-87 1.1024268799596975 2314 88-89 1.1475526049032148 2314 90-91 1.1942780629264718 2314 92-93 1.2031988376204446 2314 94-95 1.2056844636755812 2314 96-97 1.2501107423213433 2314 98-99 1.3620076596295654 2314 100 1.470250596529553 2315 1 0.40713937836295244 2315 2 0.4067126250930855 2315 3 0.4708178087998185 2315 4 0.23217915970764125 2315 5 0.29633042842878154 2315 6 0.345113301661776 2315 7 0.3913185182231018 2315 8 0.4095859543480884 2315 9 0.5487190889441109 2315 10-11 0.4220923681189461 2315 12-13 0.4753465866187554 2315 14-15 0.6952185375111597 2315 16-17 0.7177208558005503 2315 18-19 0.5519975971488051 2315 20-21 0.6311617477554705 2315 22-23 0.4903035018513364 2315 24-25 0.6798371281320854 2315 26-27 0.7558503531292118 2315 28-29 0.7943363857403511 2315 30-31 0.8094107858488542 2315 32-33 0.8276601873783349 2315 34-35 0.8904332305732225 2315 36-37 0.944296710832937 2315 38-39 0.9757240662253608 2315 40-41 0.9705084082352684 2315 42-43 1.0018449983649091 2315 44-45 0.9901322987259817 2315 46-47 1.0649505321490764 2315 48-49 1.0921994438484788 2315 50-51 1.006001191731329 2315 52-53 1.0343485793041935 2315 54-55 0.8649520250605462 2315 56-57 0.8321831187624937 2315 58-59 0.8151974715548889 2315 60-61 0.8511050614198794 2315 62-63 0.8237570777867163 2315 64-65 0.8125996059051204 2315 66-67 0.8156517311575939 2315 68-69 0.8093399690377296 2315 70-71 0.7855101174403103 2315 72-73 0.5442690853209555 2315 74-75 0.7228276458746876 2315 76-77 0.7700647899618644 2315 78-79 0.7861391132649658 2315 80-81 0.5327293603926648 2315 82-83 0.49652326145201897 2315 84-85 0.7738930695803354 2315 86-87 0.8557438822691275 2315 88-89 0.9186637588443212 2315 90-91 0.9800968417699103 2315 92-93 1.0308393341558642 2315 94-95 1.0577044901994306 2315 96-97 0.9007092990589101 2315 98-99 0.34075823100347336 2315 100 0.7804465834780636 2316 1 0.26830020673089194 2316 2 0.20222152583302488 2316 3 0.24552858994350757 2316 4 0.15184700946913665 2316 5 0.06393989392261545 2316 6 0.09175948039227677 2316 7 0.09840899585860541 2316 8 0.11529946928177282 2316 9 0.2147899900857695 2316 10-11 0.2011345628341985 2316 12-13 0.027931987404514302 2316 14-15 -0.024332385915336374 2316 16-17 0.17121333932369964 2316 18-19 0.26788221578844684 2316 20-21 0.2771883139218545 2316 22-23 0.2479719184608129 2316 24-25 0.28824807624892657 2316 26-27 0.306791627860612 2316 28-29 0.2862460496516306 2316 30-31 0.22188874480127652 2316 32-33 0.3985949345190747 2316 34-35 0.3721353327762742 2316 36-37 0.3731047467166775 2316 38-39 0.5351075201295359 2316 40-41 0.5380538493158085 2316 42-43 0.542895446575038 2316 44-45 0.42727762893991894 2316 46-47 0.2961492000150301 2316 48-49 0.20284443394950102 2316 50-51 0.36361800437870073 2316 52-53 0.4185103696904662 2316 54-55 0.11855491878972657 2316 56-57 -0.027739851620026457 2316 58-59 0.04616905844464014 2316 60-61 0.11802316723152728 2316 62-63 0.1928296772832141 2316 64-65 0.18828170846090586 2316 66-67 0.1906646436356283 2316 68-69 0.136405621156392 2316 70-71 -0.07100613491393659 2316 72-73 -0.11473451869054685 2316 74-75 0.13581018533379563 2316 76-77 0.28179507409998905 2316 78-79 0.30256007834636733 2316 80-81 0.46949803708143634 2316 82-83 0.4647263384981173 2316 84-85 0.5082683103017622 2316 86-87 0.5978871040473273 2316 88-89 0.515251484991321 2316 90-91 0.41018187061848366 2316 92-93 0.42930826895376484 2316 94-95 0.6097762753579374 2316 96-97 0.5723568160198447 2316 98-99 0.4068507859732513 2316 100 0.2786129393159307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 48.0 1 688.0 2 264.0 3 245.0 4 178.0 5 130.0 6 172.0 7 711.0 8 2559.0 9 4882.0 10 7434.0 11 11368.0 12 15725.0 13 19934.0 14 23772.0 15 28530.0 16 34765.0 17 44194.0 18 55281.0 19 68000.0 20 85571.0 21 106743.0 22 117978.0 23 115590.0 24 110882.0 25 112881.0 26 124210.0 27 142952.0 28 169490.0 29 204589.0 30 249882.0 31 314395.0 32 402395.0 33 538987.0 34 789795.0 35 1088906.0 36 1651251.0 37 2247947.0 38 1803116.0 39 539597.0 40 451.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63364819665224 20.72594816518312 12.566114974651066 23.07428866351358 2 15.617508805411893 19.73123398388167 41.62110832162665 23.030148889079793 3 19.28479219882063 26.996996513185284 28.21605985575466 25.50215143223943 4 12.355731123268917 16.064519947857814 39.48073218026528 32.09901674860799 5 12.87850067814283 38.78126914050014 34.22080423102021 14.11942595033682 6 29.54950717831246 37.93630884952711 18.306499882375295 14.207684089785136 7 27.344209982286817 32.210857082407095 22.93695523404522 17.507977701260867 8 26.730081692731012 35.08469024138306 19.708303464334882 18.476924601551048 9 27.13523466727991 15.000539974638553 21.57425265595852 36.289972702123016 10-11 26.37826842824582 26.00236429119029 26.76763841266283 20.851728867901063 12-13 26.16601760360692 23.764744700156726 27.648866611386907 22.420371084849442 14-15 23.4862583516324 24.78308709793322 25.704079312430018 26.026575238004362 16-17 21.780150261523694 27.482610334231573 26.95619386844827 23.781045535796462 18-19 21.1302576969462 27.58877901061128 29.549996337192947 21.730966955249578 20-21 22.83049577871747 26.73935577651882 28.851336706086666 21.578811738677043 22-23 23.14468826212188 26.528274061013768 28.469020005039326 21.858017671825024 24-25 22.43421357750968 26.304950649111575 29.530283728329454 21.730552045049293 26-27 22.316911531145543 26.65949283106752 28.38340753956829 22.640188098218644 28-29 21.493438471716964 27.331251732670637 28.867030454992353 22.30827934062005 30-31 22.71060779407955 26.822255592143197 28.78891798187014 21.678218631907118 32-33 22.938559815017342 26.97374148538382 27.889352583844744 22.198346115754095 34-35 22.80479892731682 26.875972698849388 28.846831312282823 21.472397061550964 36-37 22.28392151933676 26.61601065455995 28.42064165656812 22.679426169535173 38-39 22.645274330206565 26.80395472131698 28.841195478077868 21.709575470398583 40-41 22.408069701862456 25.701293272352395 29.39104784418704 22.49958918159811 42-43 22.263623615311097 26.00784324687737 28.638743264397416 23.089789873414116 44-45 22.279846719361913 26.965166320158453 28.607104647765567 22.147882312714064 46-47 22.249376779754794 26.70718451539651 27.918931417904187 23.12450728694451 48-49 23.051304110384706 26.6841051372308 27.864440397102474 22.400150355282015 50-51 22.695103330843146 26.436764828415747 27.930992060129086 22.937139780612014 52-53 22.226399243524376 27.07990064535647 27.589320666764777 23.104379444354375 54-55 22.01555596083104 26.91583808628145 27.765076689433073 23.303529263454443 56-57 23.084204661147343 26.450574015348415 27.172545231316175 23.29267609218807 58-59 22.11182955060081 26.97618035282098 28.05378468311067 22.858205413467548 60-61 22.333525756090136 27.671579307180156 27.28823667910071 22.706658257628998 62-63 22.674333804462172 26.84518377029031 26.95241954813025 23.52806287711727 64-65 21.865054782788512 26.91001803586325 27.489157874468933 23.73576930687931 66-67 22.397735338496066 26.590383263077143 27.30574826512425 23.706133133302536 68-69 21.91438812549865 26.747573275810453 27.62564058756775 23.712398011123142 70-71 21.967898905131143 26.353628782162225 27.737578341565555 23.940893971141076 72-73 22.648748050025823 26.60031434638771 27.812554784246778 22.938382819339683 74-75 22.913067683814962 26.827387159369238 27.29437437920049 22.965170777615306 76-77 22.562942487171345 26.689689470318555 26.996541995245956 23.750826047264145 78-79 22.4160706841211 26.755866254293377 27.117103087552387 23.71095997403314 80-81 22.039455456219986 27.236081800404715 27.601269649671316 23.123193093703982 82-83 23.021467912602656 26.326117634303692 28.64292497444084 22.009489478652814 84-85 23.190092732547427 26.786867876457954 27.248852836813462 22.774186554181153 86-87 21.836513435182457 27.614595839486228 27.657128534409313 22.891762190921998 88-89 22.940838958439812 26.297428428653497 27.55608772798393 23.20564488492276 90-91 22.775255842479318 26.326894887779133 27.6853618735456 23.21248739619595 92-93 23.1868581970504 26.573365353670543 27.48849874605211 22.751277703226947 94-95 21.998657457165834 26.72660782456253 28.046736371125057 23.227998347146574 96-97 22.325377566199236 26.599040739570825 27.590837029298786 23.484744664931153 98-99 21.74484240092089 27.11515841984042 27.368989091797346 23.771010087441347 100 22.572533043290775 26.684140911828713 26.449321098513046 24.29400494636747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2772.0 1 1911.5 2 1988.5 3 2656.0 4 1897.5 5 1562.0 6 2224.5 7 3984.5 8 6172.5 9 7046.0 10 6229.5 11 5676.5 12 6406.0 13 7553.5 14 9030.0 15 10716.0 16 12257.5 17 13735.5 18 15113.0 19 16089.0 20 16937.5 21 18013.0 22 19637.5 23 21860.5 24 24163.0 25 27412.5 26 32978.5 27 38940.5 28 47406.0 29 65843.0 30 94103.5 31 121418.0 32 158615.5 33 198598.5 34 227871.0 35 260333.5 36 289830.0 37 337089.5 38 356850.5 39 336642.0 40 314815.0 41 288355.0 42 281599.0 43 299595.5 44 330052.5 45 390805.5 46 451664.5 47 492892.5 48 524639.0 49 523459.5 50 506601.5 51 521559.5 52 522375.5 53 491950.0 54 458991.5 55 390632.5 56 337643.0 57 278869.5 58 218159.5 59 203830.0 60 166934.5 61 114256.0 62 85022.5 63 72084.5 64 53617.0 65 26975.5 66 15111.0 67 10119.0 68 7528.5 69 5831.5 70 4564.5 71 3616.5 72 2947.0 73 2722.0 74 2221.0 75 1720.0 76 1275.0 77 839.5 78 580.5 79 446.5 80 334.0 81 237.0 82 194.0 83 145.0 84 103.5 85 87.5 86 65.5 87 48.0 88 40.5 89 34.0 90 28.0 91 21.5 92 17.0 93 15.0 94 11.5 95 6.5 96 5.0 97 4.0 98 4.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05722428573767888 2 0.1333156765708289 3 0.016722306827542555 4 0.016223930466530114 5 0.01894720129634811 6 0.052098128881550895 7 0.05727768320493023 8 0.04895657789159744 9 0.039861209303120336 10-11 0.03888225573684589 12-13 0.040301738407943835 14-15 0.018533370925150276 16-17 0.017282980233681557 18-19 0.017220683188555 20-21 0.02085171096164567 22-23 0.025305949688194388 24-25 0.02446493957898589 26-27 0.01908514475341406 28-29 0.01801719540838739 30-31 0.01619278194396683 32-33 0.022871915139321113 34-35 0.023161151420265834 36-37 0.023250147199018054 38-39 0.0211053489310895 40-41 0.025043412140875333 42-43 0.03239446346580889 44-45 0.041574378044100614 46-47 0.04722116020592911 48-49 0.03938063209785834 50-51 0.027197109986679114 52-53 0.039167042228853 54-55 0.036252430474717726 56-57 0.03785435449225772 58-59 0.04284701768025739 60-61 0.02263162653669011 62-63 0.025190255175816502 64-65 0.02455393535773811 66-67 0.031602401034914115 68-69 0.038441726632022395 70-71 0.040079248961063275 72-73 0.043866019346970334 74-75 0.026609737846914446 76-77 0.02014864430950311 78-79 0.01929873462241939 80-81 0.023855318494533165 82-83 0.020081897475438943 84-85 0.012299216623557112 86-87 0.01425267396716839 88-89 0.018101741398202 90-91 0.02029548734444428 92-93 0.01930763420029461 94-95 0.022978710073823776 96-97 0.024291397810419058 98-99 0.026832227293795 100 0.028425251733459778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1236488E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.40969359852032 #Duplication Level Percentage of deduplicated Percentage of total 1 74.79850755348433 46.68151938039565 2 14.917100577864991 18.619433528857286 3 5.126336696622881 9.597993075572546 4 2.257437094697659 5.635438295920593 5 1.0549269388608045 3.2918833511563896 6 0.588905603237185 2.2052050953890694 7 0.34799484186388424 1.5202776018213382 8 0.22587824090196507 1.127759344421152 9 0.1509556782225027 0.8478987862340897 >10 0.48793573186353784 4.858610778999124 >50 0.025488936778054284 1.1003536466375676 >100 0.01656769040473813 1.9944845703554852 >500 0.0013037373906699518 0.5617468879031535 >1k 5.50564894592359E-4 0.6369649349200623 >5k 5.5056456145940745E-5 0.24394856214502353 >10k+ 5.5056456145940745E-5 1.0764821592715306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41495 0.3692879839323461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31247 0.2780851098670688 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21245 0.18907153195909612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.119662300177778E-5 0.0 0.0 0.0 6 0.0 7.119662300177778E-5 0.0 0.0 8.899577875222223E-6 7 0.0 7.119662300177778E-5 0.0 8.899577875222223E-6 8.899577875222223E-6 8 0.0 7.119662300177778E-5 0.0 8.899577875222223E-6 8.899577875222223E-6 9 0.0 7.119662300177778E-5 0.0 8.899577875222223E-6 8.899577875222223E-6 10-11 0.0 8.0096200877E-5 0.0 1.3349366812833335E-5 1.7799155750444446E-5 12-13 0.0 1.0234514556505556E-4 0.0 2.2248944688055556E-5 2.6698733625666666E-5 14-15 0.0 1.0679493450266666E-4 0.0 3.114852256327778E-5 3.559831150088889E-5 16-17 0.0 1.1124472344027777E-4 0.0 4.449788937611111E-5 7.119662300177778E-5 18-19 0.0 1.2459409025311112E-4 0.0 4.8947678313722225E-5 1.2014430131550001E-4 20-21 0.0 1.3349366812833333E-4 0.0 1.5129282387877778E-4 1.60192401754E-4 22-23 0.0 1.3349366812833333E-4 0.0 3.871316375721667E-4 1.7799155750444445E-4 24-25 0.0 1.3349366812833333E-4 0.0 8.899577875222222E-4 1.7799155750444445E-4 26-27 0.0 1.3349366812833333E-4 0.0 0.0016197231732904445 1.8244134644205556E-4 28-29 0.0 1.3349366812833333E-4 0.0 0.004703426907054944 1.957907132548889E-4 30-31 0.0 1.6909197962922224E-4 0.0 0.018208536332704667 2.1358986900533333E-4 32-33 0.0 1.6909197962922224E-4 0.0 0.05084773819008217 2.2248944688055557E-4 34-35 0.0 1.8244134644205558E-4 0.0 0.09707214567398639 2.2248944688055557E-4 36-37 0.0 2.1358986900533333E-4 0.0 0.17113443275158574 2.3138902475577778E-4 38-39 0.0 2.358388136933889E-4 0.0 0.28296652833162816 2.3138902475577778E-4 40-41 0.0 2.40288602631E-4 0.0 0.40638587430520995 2.3138902475577778E-4 42-43 0.0 2.40288602631E-4 0.0 0.5259517030588206 2.447383915686111E-4 44-45 0.0 2.4918818050622223E-4 0.0 0.6606557137781841 2.5363796944383334E-4 46-47 0.0 2.5808775838144445E-4 0.0 0.8178089096877956 2.5808775838144445E-4 48-49 0.0 2.5808775838144445E-4 0.0 0.9939849533056948 2.6698733625666666E-4 50-51 0.0 2.5808775838144445E-4 0.0 1.1790027275426271 2.7143712519427777E-4 52-53 0.0 2.6698733625666666E-4 0.0 1.3580043871359093 2.9813585881994446E-4 54-55 0.0 3.20384803508E-4 0.0 1.548775738469173 3.114852256327778E-4 56-57 0.0 3.3818395925844447E-4 0.0 1.7687021069216646 3.114852256327778E-4 58-59 0.0 3.648826928841111E-4 0.0 2.026496179233227 3.159350145703889E-4 60-61 0.0 3.648826928841111E-4 0.0 2.282434689557805 3.20384803508E-4 62-63 0.0 3.648826928841111E-4 0.0 2.550538922837812 3.292843813832222E-4 64-65 0.0 3.915814265097778E-4 0.0 2.8166763494073948 3.292843813832222E-4 66-67 0.0 4.4497889376111113E-4 0.0 3.1098684927176534 3.292843813832222E-4 68-69 0.0 4.6277804951155556E-4 0.0 3.4316193814295 3.292843813832222E-4 70-71 0.0 4.939265720748334E-4 0.0 3.7784003329154094 3.292843813832222E-4 72-73 0.0 5.250750946381111E-4 0.0 4.125181284401318 3.648826928841111E-4 74-75 0.0 5.250750946381111E-4 0.0 4.458439327305827 3.648826928841111E-4 76-77 0.0 5.339746725133333E-4 0.0 4.83339634234469 3.782320596969445E-4 78-79 0.0 5.56223617201389E-4 0.0 5.2298013400628385 3.915814265097778E-4 80-81 0.0 6.007215065775E-4 0.0 5.64497554751983 4.049307933226111E-4 82-83 0.0 6.051712955151111E-4 0.0 6.07587530908234 4.1828016013544444E-4 84-85 0.0 6.051712955151111E-4 0.0 6.488557634734269 4.3162952694827776E-4 86-87 0.0 6.363198180783889E-4 0.0 6.921940378523966 4.360793158858889E-4 88 0.0 6.40769607016E-4 0.0 7.26593576213493 4.360793158858889E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21880 0.0 44.134617 1 GTATCAA 38555 0.0 39.998623 1 GTACTAG 12115 0.0 38.14498 1 GGGCACC 12610 0.0 36.7471 94 GCATCGT 11800 0.0 36.171745 8 TACCTGG 6125 0.0 35.496315 2 CAACGCA 43925 0.0 33.208645 5 ATCAACG 44510 0.0 32.8354 3 ACCTGGG 6420 0.0 32.51076 3 CTGTGCG 16215 0.0 32.265617 9 TAGGCAT 15175 0.0 31.90727 5 GTGGTAT 6385 0.0 31.76623 1 TAGTACT 17100 0.0 31.31171 4 TATCAAC 48935 0.0 31.283247 2 ACTGTGC 17570 0.0 31.088518 8 GTACATG 58375 0.0 30.876013 1 TACATGG 59190 0.0 30.05321 2 CTAGGCA 16855 0.0 29.95399 4 AACGCAG 50640 0.0 29.6875 6 CTAACGC 5655 0.0 29.59352 3 >>END_MODULE