FastQCFastQC Report
Mon 6 Jun 2016
ERR1211199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236488
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372260.33129568598302245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305130.2715528197066557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206670.18392757594721768No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC129520.11526733263987822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230150.044.8437731
GTATCAA393900.039.1531261
GGGCACC122950.037.77517394
GTACTAG121700.036.703851
TACCTGG62400.034.41942
ACCTGGG62150.033.72923
TAGGCAT148150.032.5722475
CAACGCA454900.032.5057145
ATCAACG457950.032.3196683
GTGGTAT63250.032.040211
CTGTGCG164150.031.2004369
TATCAAC510450.030.2358112
ACTGTGC178500.030.1136138
CTAGGCA167200.030.022534
TAGTACT173750.029.5670244
TACCCCG7050.029.32535
AACGCAG522950.029.13846
GTACATG572050.028.570931
TACATGG575800.028.1754472
GCATCGT151100.027.8935018
Produced by FastQC (version 0.11.5)