FastQCFastQC Report
Mon 6 Jun 2016
ERR1211198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9136905
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296650.32467230424306703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227600.24909966777590442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144800.15847817176604112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152450.046.767551
GTATCAA270900.040.8416251
GTACTAG64300.037.0338551
TACCTGG62600.034.0538832
CAACGCA315250.033.62585
ATCAACG319800.033.2208823
TATCAAC342150.031.9365792
GCATCGT66100.031.4317598
TAGGCAT81200.030.4516545
AACGCAG358450.030.1894726
TCAACGC363300.029.5537014
GTACATG388650.029.2548351
CTAGGCA90550.028.449414
TACATGG394850.028.3414752
GTGGTAT55800.028.2533531
ACATGGG393100.027.504643
ACGCAGA387050.027.3149997
ACCTGGG76300.026.9239063
CGCAGAG392450.026.9013028
GTACCTG81550.026.5456431
Produced by FastQC (version 0.11.5)