##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9136905 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.463355151443515 31.0 31.0 34.0 30.0 34.0 2 31.47616123840622 31.0 31.0 34.0 28.0 34.0 3 31.540736387212082 33.0 31.0 34.0 28.0 34.0 4 35.14101153508765 37.0 35.0 37.0 32.0 37.0 5 35.19437697995109 37.0 35.0 37.0 32.0 37.0 6 35.25988253133857 37.0 35.0 37.0 32.0 37.0 7 35.17519356937606 37.0 35.0 37.0 32.0 37.0 8 35.17946503766866 37.0 35.0 37.0 32.0 37.0 9 36.74711141245312 39.0 37.0 39.0 32.0 39.0 10-11 36.69661865806857 39.0 37.0 39.0 32.0 39.0 12-13 36.578635818146296 39.0 37.0 39.0 32.0 39.0 14-15 37.76961334281138 40.0 37.0 41.0 32.0 41.0 16-17 37.744589989717525 40.0 37.0 41.0 32.0 41.0 18-19 37.752402098960204 40.0 37.0 41.0 32.0 41.0 20-21 37.71695240346703 40.0 37.0 41.0 32.0 41.0 22-23 37.60638159201611 40.0 37.0 41.0 32.0 41.0 24-25 37.50536220963225 40.0 37.0 41.0 31.5 41.0 26-27 37.35605765847407 40.0 36.5 41.0 31.0 41.0 28-29 37.23369981410554 40.0 36.0 41.0 31.0 41.0 30-31 37.07920647090016 39.5 36.0 41.0 30.5 41.0 32-33 36.89570423463963 39.0 36.0 41.0 30.0 41.0 34-35 36.7457946646047 39.0 35.0 41.0 30.0 41.0 36-37 36.63774166416309 39.0 35.0 41.0 30.0 41.0 38-39 36.49154358067639 39.0 35.0 41.0 30.0 41.0 40-41 36.32075516818879 39.0 35.0 40.0 29.0 41.0 42-43 36.11166510979374 39.0 35.0 40.0 28.0 41.0 44-45 35.88312070662877 38.0 35.0 40.0 27.5 41.0 46-47 35.64000424651455 38.0 34.0 40.0 27.0 41.0 48-49 35.42590992245186 38.0 34.0 40.0 26.0 41.0 50-51 34.733706818665624 37.5 33.5 39.5 25.5 40.5 52-53 34.83850915599976 38.0 33.5 39.5 26.0 40.5 54-55 35.31166516451687 38.0 34.0 40.0 26.0 41.0 56-57 35.253822382962284 38.0 34.0 40.0 26.0 41.0 58-59 35.02455284365986 37.5 34.0 40.0 26.0 41.0 60-61 34.78880649410276 37.0 34.0 40.0 26.0 41.0 62-63 34.47440484496665 36.5 33.0 40.0 26.0 41.0 64-65 34.21406597748363 36.0 33.0 39.0 26.0 41.0 66-67 34.03060615164544 35.5 33.0 39.0 25.5 41.0 68-69 33.728991655270576 35.0 33.0 39.0 25.0 41.0 70-71 33.42944262854873 35.0 33.0 38.0 25.0 40.0 72-73 33.10289485334476 35.0 32.0 37.0 24.0 40.0 74-75 32.78119445260731 35.0 32.0 37.0 24.0 39.0 76-77 32.46903935194686 35.0 32.0 36.0 23.0 39.0 78-79 32.14750443394125 35.0 31.0 36.0 23.0 39.0 80-81 31.871061097822512 35.0 31.0 35.5 22.0 37.5 82-83 31.59853539026618 35.0 31.0 35.0 21.0 37.0 84-85 31.36906972328157 34.0 31.0 35.0 20.0 37.0 86-87 31.15776518416247 34.0 31.0 35.0 20.0 36.0 88-89 30.95913435676523 34.0 30.5 35.0 20.0 36.0 90-91 30.74159039631035 34.0 30.0 35.0 19.0 36.0 92-93 30.552131985612196 34.0 30.0 35.0 19.0 35.0 94-95 30.387266421178726 34.0 29.5 35.0 18.0 35.0 96-97 30.20671157246354 34.0 29.0 35.0 18.0 35.0 98-99 29.978254945192052 34.0 29.0 35.0 17.0 35.0 100 28.82927917057253 33.0 26.0 35.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3707709143194897 1101 2 -0.39538279016802846 1101 3 -0.34609935392422386 1101 4 -0.27655142484967854 1101 5 -0.210555869016396 1101 6 -0.2652429734306949 1101 7 -0.22857816794439856 1101 8 -0.2453376155403859 1101 9 -0.47620311902687007 1101 10-11 -0.4356707887907092 1101 12-13 -0.4497253976195097 1101 14-15 -0.6283964738342789 1101 16-17 -0.6511401038557381 1101 18-19 -0.63277212130874 1101 20-21 -0.6786187764709837 1101 22-23 -0.7105847390978823 1101 24-25 -0.8600679760729477 1101 26-27 -0.7779281429765419 1101 28-29 -0.8415307243654198 1101 30-31 -0.8401769399592709 1101 32-33 -0.8008825943918154 1101 34-35 -0.8709536997343932 1101 36-37 -0.9140274324855184 1101 38-39 -0.9966230192782319 1101 40-41 -0.9784793004163035 1101 42-43 -1.051816142249784 1101 44-45 -1.0203314582241774 1101 46-47 -1.161436579246299 1101 48-49 -1.1354175266428328 1101 50-51 -1.093989546614985 1101 52-53 -0.9806464280961933 1101 54-55 -0.8512812708877462 1101 56-57 -0.8222571581563258 1101 58-59 -0.7837271906759256 1101 60-61 -0.7894253883085725 1101 62-63 -0.8754107932960693 1101 64-65 -0.881028404342139 1101 66-67 -0.8206657928906225 1101 68-69 -0.8324170696646433 1101 70-71 -0.9539597485723093 1101 72-73 -0.9502891969401404 1101 74-75 -0.9456272385406024 1101 76-77 -1.0461997676283978 1101 78-79 -1.0566484126367328 1101 80-81 -1.066810486570521 1101 82-83 -1.0644509935772284 1101 84-85 -1.0312145117313491 1101 86-87 -1.0820371697869469 1101 88-89 -1.2101794272515782 1101 90-91 -1.2921493184732356 1101 92-93 -1.3949832949830494 1101 94-95 -1.4304557872240515 1101 96-97 -1.4779963670292524 1101 98-99 -1.5917519858149767 1101 100 -1.701069458680692 1102 1 -0.42493106527439295 1102 2 -0.39441930988207474 1102 3 -0.37170182623279047 1102 4 -0.27153834429676493 1102 5 -0.26134546435893924 1102 6 -0.3109017983260074 1102 7 -0.285299033889018 1102 8 -0.32723752905942405 1102 9 -0.43000082599939304 1102 10-11 -0.49048881757632046 1102 12-13 -0.4799412005231076 1102 14-15 -0.5521086075594184 1102 16-17 -0.6572180859946215 1102 18-19 -0.6612514062178221 1102 20-21 -0.7343050863179599 1102 22-23 -0.7927264677672383 1102 24-25 -0.7698945850711212 1102 26-27 -0.7326591097765913 1102 28-29 -0.8502916933658469 1102 30-31 -0.820615315914047 1102 32-33 -0.8712275769947979 1102 34-35 -0.8822233639381025 1102 36-37 -0.8805716860701196 1102 38-39 -0.958517654182657 1102 40-41 -0.8951100945383175 1102 42-43 -0.9470333917491374 1102 44-45 -1.0613825715145353 1102 46-47 -1.0117579876852858 1102 48-49 -1.0173629348592996 1102 50-51 -1.0073832508484415 1102 52-53 -0.890400335894185 1102 54-55 -0.7250686998154308 1102 56-57 -0.7926151615185191 1102 58-59 -0.8166522734901989 1102 60-61 -0.7435856598805373 1102 62-63 -0.7987303920231028 1102 64-65 -0.7982524358448373 1102 66-67 -0.8324506905368239 1102 68-69 -0.8998590569384533 1102 70-71 -0.9066344003534184 1102 72-73 -0.9370106702358143 1102 74-75 -0.9769465110720184 1102 76-77 -1.0571244302540102 1102 78-79 -0.9846634718831773 1102 80-81 -1.0949061136032547 1102 82-83 -1.0056897281824284 1102 84-85 -1.036635219124836 1102 86-87 -1.091155584516276 1102 88-89 -1.2178223643200532 1102 90-91 -1.189410880122665 1102 92-93 -1.1734428435572113 1102 94-95 -1.2074345553993346 1102 96-97 -1.3118227079045184 1102 98-99 -1.379947177527491 1102 100 -1.5426276181625482 1103 1 -0.48628809727279076 1103 2 -0.44795764483053446 1103 3 -0.514695178564395 1103 4 -0.3915978888405718 1103 5 -0.3540649982220003 1103 6 -0.42856421549518586 1103 7 -0.4117407151949024 1103 8 -0.392595209205453 1103 9 -0.5279140504608648 1103 10-11 -0.5900887513385626 1103 12-13 -0.6001522162067943 1103 14-15 -0.7595623821805262 1103 16-17 -0.7873333960235058 1103 18-19 -0.7332435920174518 1103 20-21 -0.8258217661513925 1103 22-23 -0.828320392659613 1103 24-25 -0.8262081453717727 1103 26-27 -0.883433008552096 1103 28-29 -0.9155055438771953 1103 30-31 -0.8789529501435922 1103 32-33 -0.8844332577267551 1103 34-35 -0.8959569133035714 1103 36-37 -0.8944188761442291 1103 38-39 -0.8760538598690957 1103 40-41 -0.9803703738107075 1103 42-43 -1.0443593089044327 1103 44-45 -1.0925200758754912 1103 46-47 -1.108117780942301 1103 48-49 -1.1226093931105723 1103 50-51 -1.2539694226297868 1103 52-53 -1.091648226422521 1103 54-55 -0.8873489907100307 1103 56-57 -0.8046422432406288 1103 58-59 -0.8342663485337383 1103 60-61 -0.8190952717912552 1103 62-63 -0.7585390629628534 1103 64-65 -0.8280567109980481 1103 66-67 -0.9018179979548435 1103 68-69 -0.8058735732654014 1103 70-71 -0.7640677556488455 1103 72-73 -0.8129354293288245 1103 74-75 -0.8807206220309141 1103 76-77 -0.8291437512235973 1103 78-79 -0.8893874276305809 1103 80-81 -0.9029859181822211 1103 82-83 -0.9600158966251371 1103 84-85 -1.084009435004301 1103 86-87 -1.0004287687968123 1103 88-89 -1.0950347170394679 1103 90-91 -1.2201640964599534 1103 92-93 -1.223188658207338 1103 94-95 -1.1928807751189616 1103 96-97 -1.391780024477125 1103 98-99 -1.450217733628282 1103 100 -1.4508429804823813 1104 1 -0.42317763893196414 1104 2 -0.45959129832647605 1104 3 -0.44968328853633466 1104 4 -0.36783880611328357 1104 5 -0.36133031502460966 1104 6 -0.3655146636621396 1104 7 -0.43553315114834135 1104 8 -0.3657732310857398 1104 9 -0.4811863933361167 1104 10-11 -0.4944621590836178 1104 12-13 -0.530271797449096 1104 14-15 -0.6730747015179261 1104 16-17 -0.7435231818929608 1104 18-19 -0.7224273690876686 1104 20-21 -0.6749234823734156 1104 22-23 -0.6031088733628636 1104 24-25 -0.7809443857337257 1104 26-27 -0.6884817354600159 1104 28-29 -0.752450611851124 1104 30-31 -0.7581808615299721 1104 32-33 -0.8395033990508409 1104 34-35 -1.029103034638176 1104 36-37 -0.9585376952539733 1104 38-39 -0.8705375028627387 1104 40-41 -0.993135080300334 1104 42-43 -0.9878229387712096 1104 44-45 -1.0145545557373268 1104 46-47 -1.0130887744129495 1104 48-49 -1.080684799082583 1104 50-51 -1.0896261087973116 1104 52-53 -0.8793971156551521 1104 54-55 -0.7784036270949102 1104 56-57 -0.7488193088249275 1104 58-59 -0.7656117601026224 1104 60-61 -0.845239982307092 1104 62-63 -0.800635974902228 1104 64-65 -0.7636677406830543 1104 66-67 -0.8664425977515791 1104 68-69 -0.9905082907746348 1104 70-71 -0.8996187339279018 1104 72-73 -0.809394293768321 1104 74-75 -0.8331767397074508 1104 76-77 -0.8730261810446933 1104 78-79 -0.937408252579516 1104 80-81 -1.0132959279108142 1104 82-83 -0.9692104891028812 1104 84-85 -0.9234153811471266 1104 86-87 -0.8732550871468909 1104 88-89 -1.0547993036730254 1104 90-91 -1.0435495464780757 1104 92-93 -1.105665410640011 1104 94-95 -1.1600696310687049 1104 96-97 -1.1665291676594052 1104 98-99 -1.1888238773492645 1104 100 -1.1656670544099228 1105 1 -0.538724065751623 1105 2 -0.49285517277610325 1105 3 -0.5415032753365061 1105 4 -0.33253382306206447 1105 5 -0.297434201537655 1105 6 -0.3095673677223374 1105 7 -0.3327928813948233 1105 8 -0.4073058857865064 1105 9 -0.5262556668863922 1105 10-11 -0.5287038589698128 1105 12-13 -0.6339079613546943 1105 14-15 -0.7401583513797547 1105 16-17 -0.775108156817879 1105 18-19 -0.7844758458994434 1105 20-21 -0.7524601436253988 1105 22-23 -0.7248054894014331 1105 24-25 -0.7664500057075472 1105 26-27 -0.7699001103872263 1105 28-29 -0.7729239676299571 1105 30-31 -0.7599619924521761 1105 32-33 -0.773436259077215 1105 34-35 -0.7896630384799508 1105 36-37 -0.8121258654635142 1105 38-39 -0.8267043011360116 1105 40-41 -0.8381374450754677 1105 42-43 -0.9269023492939539 1105 44-45 -1.0353129219808253 1105 46-47 -1.0198474632444743 1105 48-49 -1.0662283797710543 1105 50-51 -0.9829047605371741 1105 52-53 -0.8713882705106357 1105 54-55 -0.8391404299495804 1105 56-57 -0.7121973798893109 1105 58-59 -0.7118294949852171 1105 60-61 -0.7664499788802743 1105 62-63 -0.8222511109276027 1105 64-65 -0.8383808697885868 1105 66-67 -0.8320965165432952 1105 68-69 -0.8643208512386025 1105 70-71 -0.8415022066529758 1105 72-73 -0.817319504717787 1105 74-75 -0.7689683337095019 1105 76-77 -0.750666345200731 1105 78-79 -0.8207633420143949 1105 80-81 -0.8839072506653274 1105 82-83 -0.9761120095579336 1105 84-85 -0.9513912783780611 1105 86-87 -0.9556258502841182 1105 88-89 -0.9503998810618803 1105 90-91 -1.02572197446014 1105 92-93 -1.001030205870407 1105 94-95 -1.1132422655570586 1105 96-97 -1.0990490518365092 1105 98-99 -1.1261433439954054 1105 100 -1.136642703526789 1106 1 -0.5509021720100087 1106 2 -0.5527247601104079 1106 3 -0.5963728844861507 1106 4 -0.4543858694081422 1106 5 -0.45759156677702606 1106 6 -0.41318816608773545 1106 7 -0.4548813584871496 1106 8 -0.5057616924931949 1106 9 -0.6280446199111367 1106 10-11 -0.5706342651804093 1106 12-13 -0.660868233325985 1106 14-15 -0.8086086813172031 1106 16-17 -0.8768627786495102 1106 18-19 -0.889077957020298 1106 20-21 -0.7960628774903213 1106 22-23 -0.7691395056481625 1106 24-25 -0.813254937909953 1106 26-27 -0.9099923196368422 1106 28-29 -0.9721005727691647 1106 30-31 -0.9342575932824602 1106 32-33 -0.9094569006965898 1106 34-35 -0.9069777946336686 1106 36-37 -1.02215180586132 1106 38-39 -0.9722120778015224 1106 40-41 -1.0272429772332927 1106 42-43 -0.9974524197519088 1106 44-45 -1.0607064889053603 1106 46-47 -1.0046499040790309 1106 48-49 -1.0332350543703015 1106 50-51 -1.049111452611065 1106 52-53 -1.0176724355715407 1106 54-55 -0.8428238593188908 1106 56-57 -0.8360993270930592 1106 58-59 -0.8532818697472635 1106 60-61 -0.8464424699281494 1106 62-63 -0.818127172495636 1106 64-65 -0.9053497073576082 1106 66-67 -0.9632639440805733 1106 68-69 -0.996631011067862 1106 70-71 -0.8912153824698521 1106 72-73 -0.7894349824894107 1106 74-75 -0.812821426576626 1106 76-77 -0.7845070077986307 1106 78-79 -0.7742196065635483 1106 80-81 -0.8371844616109421 1106 82-83 -0.9388504498326746 1106 84-85 -0.8478357792005546 1106 86-87 -0.9028974098259006 1106 88-89 -0.8426970758663863 1106 90-91 -0.8647123425956948 1106 92-93 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1107 50-51 -1.056079356004794 1107 52-53 -0.9013219536184707 1107 54-55 -0.8822042798834602 1107 56-57 -0.8915260005255732 1107 58-59 -0.9060363025548881 1107 60-61 -0.9113433033964498 1107 62-63 -0.8695841288769088 1107 64-65 -0.8514619889651769 1107 66-67 -0.8592550309910578 1107 68-69 -0.8015102215530305 1107 70-71 -0.8952863274209264 1107 72-73 -0.8326223096441225 1107 74-75 -0.775273609927094 1107 76-77 -0.7483562173657177 1107 78-79 -0.6607463043665476 1107 80-81 -0.784406812698748 1107 82-83 -0.7768145784001206 1107 84-85 -0.8555082382503549 1107 86-87 -0.7750260357876222 1107 88-89 -0.8242166168027332 1107 90-91 -0.8870854407020943 1107 92-93 -0.8851278609608357 1107 94-95 -0.9696118460266128 1107 96-97 -1.0115591381764162 1107 98-99 -1.0026773496823829 1107 100 -0.9966220442783893 1108 1 -0.5839409797761093 1108 2 -0.585912803710336 1108 3 -0.6120621862471829 1108 4 -0.4685473416512025 1108 5 -0.4244741376056993 1108 6 -0.4487291950383394 1108 7 -0.4844317205664552 1108 8 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1.1421069814352123 2310 34-35 1.2049368725489984 2310 36-37 1.2195795906446492 2310 38-39 1.1962628779702627 2310 40-41 1.1944820614663954 2310 42-43 1.2872459990893148 2310 44-45 1.374170916003969 2310 46-47 1.3067720331169 2310 48-49 1.355869600649605 2310 50-51 1.346661988344259 2310 52-53 1.2387890305938782 2310 54-55 1.1282239347987542 2310 56-57 1.1620209736588194 2310 58-59 1.0936235371523395 2310 60-61 1.0965100400231975 2310 62-63 1.0997465559430992 2310 64-65 1.078144183619841 2310 66-67 1.0970211897097855 2310 68-69 1.0647736494805713 2310 70-71 1.0261676891798643 2310 72-73 0.9945378171567612 2310 74-75 0.9908778491863117 2310 76-77 1.0492121706622228 2310 78-79 1.058075976143222 2310 80-81 1.0870989030507126 2310 82-83 1.1379027843557346 2310 84-85 1.1569222095212517 2310 86-87 1.0892417155610907 2310 88-89 1.068221074288413 2310 90-91 1.1832407202083743 2310 92-93 1.2473956758155076 2310 94-95 1.3051733363652396 2310 96-97 1.3337542517470595 2310 98-99 1.3257607339781359 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1.1361800385170042 2311 60-61 1.1634464755525542 2311 62-63 1.1283562049436995 2311 64-65 1.088192115505457 2311 66-67 1.0966756836036353 2311 68-69 1.1206615708873855 2311 70-71 1.068438411388307 2311 72-73 1.069571535018234 2311 74-75 1.102463768365375 2311 76-77 1.1373791949328549 2311 78-79 1.1577544304801464 2311 80-81 1.0869052121085296 2311 82-83 1.1362411587927532 2311 84-85 1.1322105643792604 2311 86-87 1.1477118284373269 2311 88-89 1.212624399675267 2311 90-91 1.2956616204977323 2311 92-93 1.284728860167558 2311 94-95 1.2560120206197602 2311 96-97 1.296436118293073 2311 98-99 1.4479326088177267 2311 100 1.489606421695779 2312 1 0.6186143195378726 2312 2 0.5792594963061077 2312 3 0.649931737549327 2312 4 0.3575430603943133 2312 5 0.45537029374075644 2312 6 0.5126558643768675 2312 7 0.3305196807618458 2312 8 0.4606053724881747 2312 9 0.6615506239453595 2312 10-11 0.6614159672158806 2312 12-13 0.7725117566600019 2312 14-15 1.0115586150904434 2312 16-17 0.9675028457817163 2312 18-19 0.949330892028108 2312 20-21 1.0302090654146525 2312 22-23 1.0431891336104329 2312 24-25 1.0465506023704307 2312 26-27 1.1014529266711364 2312 28-29 1.0977563651517315 2312 30-31 1.0931107100464672 2312 32-33 1.1377018597283524 2312 34-35 1.1099248933644859 2312 36-37 1.1183898946968398 2312 38-39 1.194069018011291 2312 40-41 1.239718197841519 2312 42-43 1.2975971132685729 2312 44-45 1.286428100682464 2312 46-47 1.314660544530284 2312 48-49 1.2791050265742214 2312 50-51 1.2727988380456736 2312 52-53 1.1640180098138302 2312 54-55 1.0868602456529075 2312 56-57 0.983841406296527 2312 58-59 0.9507682577999788 2312 60-61 1.0162121493560363 2312 62-63 1.034547562991989 2312 64-65 1.0568820479372292 2312 66-67 1.0154413037159244 2312 68-69 1.047242640709129 2312 70-71 0.9968141846102654 2312 72-73 0.9892588478713478 2312 74-75 1.0461023763805244 2312 76-77 1.0448658083605054 2312 78-79 1.0105213532259185 2312 80-81 1.008738874507781 2312 82-83 1.0306714451009888 2312 84-85 1.0786277667503121 2312 86-87 1.1554579551828432 2312 88-89 1.080632925195335 2312 90-91 1.1245048714847314 2312 92-93 1.1495210615052294 2312 94-95 1.265576714007988 2312 96-97 1.249189818251267 2312 98-99 0.9677049475283859 2312 100 1.1650358333888455 2313 1 0.5944269041049353 2313 2 0.6282429631360635 2313 3 0.6152864007131491 2313 4 0.49331085528029206 2313 5 0.31570994470716585 2313 6 0.46328598161027656 2313 7 0.5415250041660613 2313 8 0.5879448555550084 2313 9 0.6833578159730962 2313 10-11 0.7064451521128277 2313 12-13 0.8426055088204762 2313 14-15 0.9533647454002718 2313 16-17 0.9599751423516167 2313 18-19 0.9161559110402564 2313 20-21 0.9565659655934127 2313 22-23 1.0053722231601583 2313 24-25 0.9695118570740888 2313 26-27 0.992491039874956 2313 28-29 1.097103902460006 2313 30-31 1.0939032948110636 2313 32-33 1.0912294792086499 2313 34-35 1.1393283066641047 2313 36-37 1.1733924378816027 2313 38-39 1.1984430488850606 2313 40-41 1.232597280517247 2313 42-43 1.3026927419537273 2313 44-45 1.2637548678255044 2313 46-47 1.3055149558447425 2313 48-49 1.2862333512327453 2313 50-51 1.3126453580800401 2313 52-53 1.21309283376619 2313 54-55 1.1246024654828375 2313 56-57 1.0397809772637245 2313 58-59 1.0656689748299328 2313 60-61 1.0233046718990195 2313 62-63 1.0195878341991786 2313 64-65 1.0124898937856628 2313 66-67 1.0025031598951202 2313 68-69 0.9670310872429866 2313 70-71 1.003140048058519 2313 72-73 1.0398422988989964 2313 74-75 0.9833221322347825 2313 76-77 0.7905047403216727 2313 78-79 0.9311721369218802 2313 80-81 1.0324620969944753 2313 82-83 1.1190267452955531 2313 84-85 1.1012602592689085 2313 86-87 1.1147046539808692 2313 88-89 1.116719605820709 2313 90-91 1.1751475811863656 2313 92-93 1.231394944232317 2313 94-95 1.277342029906098 2313 96-97 1.3041423619298484 2313 98-99 1.3297612482069 2313 100 1.4714253999116487 2314 1 0.5334853158187194 2314 2 0.5246245689277487 2314 3 0.6167915739516694 2314 4 0.44722179802825224 2314 5 0.488611678912811 2314 6 0.4604153246986229 2314 7 0.47948767216274746 2314 8 0.5163242676125961 2314 9 0.6284429998462571 2314 10-11 0.621609230855185 2314 12-13 0.6976568072363065 2314 14-15 0.8732056203842546 2314 16-17 0.8478145685023932 2314 18-19 0.7913038190965054 2314 20-21 0.8414143401361613 2314 22-23 0.9243104311439154 2314 24-25 0.8930745231412871 2314 26-27 0.9043369297737982 2314 28-29 0.915752415661494 2314 30-31 0.9274479135679101 2314 32-33 0.8753595006570123 2314 34-35 0.9250493288269652 2314 36-37 0.960218169763408 2314 38-39 0.9505668308221118 2314 40-41 1.050627684737485 2314 42-43 1.1129923256867542 2314 44-45 1.1331292860472146 2314 46-47 1.1088714469855887 2314 48-49 1.0801850309336913 2314 50-51 1.10048292660408 2314 52-53 1.0904869338445735 2314 54-55 0.9245640962812018 2314 56-57 0.8921200497963753 2314 58-59 0.8646096377884191 2314 60-61 0.8603435699584736 2314 62-63 0.9043587319134474 2314 64-65 0.8901340755203364 2314 66-67 0.8903591014956405 2314 68-69 0.8709054870288426 2314 70-71 0.9456772189298519 2314 72-73 0.9380019400857904 2314 74-75 0.8878041180296634 2314 76-77 0.8983808455558844 2314 78-79 0.8771875589736808 2314 80-81 0.9666435984088473 2314 82-83 0.9725369651665368 2314 84-85 1.0177176435110873 2314 86-87 1.043519947536776 2314 88-89 1.0841200862563376 2314 90-91 1.1018244110288151 2314 92-93 1.1341829875522045 2314 94-95 1.1820471332734037 2314 96-97 1.167285987053802 2314 98-99 1.2605089939285428 2314 100 1.3381512628399719 2315 1 0.440720727025127 2315 2 0.4757844951940413 2315 3 0.5177746848606759 2315 4 0.18997241627362627 2315 5 0.34937180737034623 2315 6 0.40967558340673094 2315 7 0.45268995839298753 2315 8 0.44611681490129484 2315 9 0.5632479091523095 2315 10-11 0.44466599704432497 2315 12-13 0.5143743829819911 2315 14-15 0.7457428503861863 2315 16-17 0.7285719490100249 2315 18-19 0.5990780557309634 2315 20-21 0.7895011256424525 2315 22-23 0.6428111429742529 2315 24-25 0.7606505879807557 2315 26-27 0.86416662227996 2315 28-29 0.8900400463139064 2315 30-31 0.9098110264519619 2315 32-33 0.9202978744660442 2315 34-35 0.9440545633668194 2315 36-37 0.9762462782900698 2315 38-39 1.0267819702126957 2315 40-41 1.0611549595770384 2315 42-43 1.145028167217582 2315 44-45 1.1757633843796427 2315 46-47 1.209648628454886 2315 48-49 1.2102981341171812 2315 50-51 1.115347677436553 2315 52-53 1.1540455170720278 2315 54-55 1.0184635418101848 2315 56-57 0.9945879041788785 2315 58-59 1.028729087515707 2315 60-61 0.9907644653305283 2315 62-63 0.9974496304677132 2315 64-65 1.0493936679261537 2315 66-67 1.0413509798660456 2315 68-69 1.0525963703446166 2315 70-71 0.9770531742889759 2315 72-73 0.7343063925548776 2315 74-75 0.9416960744496805 2315 76-77 0.9848462765435215 2315 78-79 0.9462665462988937 2315 80-81 0.7559555390207571 2315 82-83 0.7328320065306677 2315 84-85 0.9560112840466921 2315 86-87 1.0197375344673212 2315 88-89 1.0891564117048027 2315 90-91 1.1760539629781341 2315 92-93 1.2166152046297753 2315 94-95 1.2504312967022209 2315 96-97 1.0300452164450178 2315 98-99 0.5269759152401079 2315 100 1.0496247810727297 2316 1 0.349882635729859 2316 2 0.34059041640301757 2316 3 0.37237126600455994 2316 4 0.2553134026225621 2316 5 0.20010114850882843 2316 6 0.2730855718187257 2316 7 0.21428578838010992 2316 8 0.25553368859764447 2316 9 0.3064720876537024 2316 10-11 0.349331329030683 2316 12-13 0.05618155556223314 2316 14-15 0.10004495463529395 2316 16-17 0.2980017902664187 2316 18-19 0.32256273298610694 2316 20-21 0.3485412298557691 2316 22-23 0.3271478791207798 2316 24-25 0.4055955178052528 2316 26-27 0.4691566156158373 2316 28-29 0.41011972846091993 2316 30-31 0.26419246394879536 2316 32-33 0.49460912950361546 2316 34-35 0.5004311874287524 2316 36-37 0.4593052817115577 2316 38-39 0.5812787172057128 2316 40-41 0.6025000247895207 2316 42-43 0.535072666530489 2316 44-45 0.5626394982971092 2316 46-47 0.3705350386179731 2316 48-49 0.29737720394675904 2316 50-51 0.3863968321727569 2316 52-53 0.5229679055137737 2316 54-55 0.2954254179891791 2316 56-57 0.08570479564109235 2316 58-59 0.12857777721222163 2316 60-61 0.16594619298845714 2316 62-63 0.31732158819401945 2316 64-65 0.30329948168692766 2316 66-67 0.29369354552865445 2316 68-69 0.2785273334407421 2316 70-71 0.02550479083958379 2316 72-73 -0.009876878331589012 2316 74-75 0.1794177583211578 2316 76-77 0.29410265820377646 2316 78-79 0.2985639695343849 2316 80-81 0.48043489442383347 2316 82-83 0.4956633223032334 2316 84-85 0.5642396595724151 2316 86-87 0.6035926954515993 2316 88-89 0.49435151067014615 2316 90-91 0.3366233266701464 2316 92-93 0.4543378749480915 2316 94-95 0.6106117768528101 2316 96-97 0.5244882216503299 2316 98-99 0.41021121349423595 2316 100 0.2480883160296301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 23.0 1 553.0 2 209.0 3 188.0 4 159.0 5 152.0 6 132.0 7 594.0 8 2102.0 9 3909.0 10 6475.0 11 9749.0 12 14482.0 13 18346.0 14 21708.0 15 25650.0 16 30223.0 17 37740.0 18 45716.0 19 55270.0 20 66964.0 21 81884.0 22 91234.0 23 93380.0 24 92978.0 25 95651.0 26 105661.0 27 121122.0 28 143614.0 29 171512.0 30 210081.0 31 261748.0 32 334305.0 33 442213.0 34 633347.0 35 877299.0 36 1320332.0 37 1833213.0 38 1498112.0 39 388589.0 40 286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16036088777745 19.53547985518145 12.782697816606605 23.521461440434496 2 15.842990936069187 19.35553125317471 41.11908043628996 23.68239737446614 3 19.588732990716643 25.673176777887374 28.37430171205814 26.363788519337838 4 12.907132259462125 15.689149335411107 37.82606868079 33.57764972433676 5 13.54773763950341 37.6601051036702 33.99205116138494 14.800106095441453 6 31.253573198989994 36.508142650480615 17.729582380982155 14.508701769547239 7 28.268476786918285 31.6798092461603 22.112254482127174 17.939459484794238 8 26.73446817643284 35.27916658454274 19.471384740718353 18.51498049830606 9 26.912143958765316 15.630558653504842 20.814741075552032 36.64255631217782 10-11 26.31845983642334 25.769408708822194 26.867959866176793 21.044171588577672 12-13 26.181691611936962 23.347801825507762 27.58679247669064 22.883714085864636 14-15 23.486224150869745 24.573064245844762 25.362222105823225 26.57848949746227 16-17 22.525790459247315 27.411734899097258 26.37833224314915 23.68414239850628 18-19 22.1240351411171 27.04117641617768 28.64388659489739 22.19090184780783 20-21 23.268373537318116 26.347335083330865 28.25495791735588 22.129333461995135 22-23 23.516596321953763 25.965335653726342 28.05807923108683 22.45998879323307 24-25 22.95729047273428 25.937345712462218 28.597615912019293 22.50774790278421 26-27 22.80530976793451 26.24994642975308 27.929389406462462 23.015354395849943 28-29 22.334801448666955 26.63691758175297 28.31131342775466 22.716967541825415 30-31 23.16716072184946 26.298287095967517 28.194395010952118 22.34015717123091 32-33 23.262550212096485 26.436610986104846 27.6497966502116 22.65104215158707 34-35 23.220863328253902 26.414616283957752 28.129296717636137 22.23522367015221 36-37 22.969385887399408 26.229738084999987 27.839612859381063 22.961263168219546 38-39 23.132330558087926 26.381678563703222 28.089502102233073 22.396488775975783 40-41 22.921631798240224 25.65199278425357 28.522948835624245 22.903426581881956 42-43 22.870572269282093 25.921838681802463 27.92090613326379 23.286682915651653 44-45 22.754466923726028 26.54797023507059 27.969827769790523 22.727735071412862 46-47 22.807065856664742 26.338526939710448 27.463710078979076 23.390697124645733 48-49 23.350006624061 26.323323154542077 27.364308026500623 22.9623621948963 50-51 23.05028334046935 26.31347463116694 27.44838559483326 23.18785643353046 52-53 22.782302687940614 26.683223606109514 27.19668909386341 23.33778461208647 54-55 22.658791055902007 26.544882611163604 27.337215446901897 23.459110886032487 56-57 23.297587282536465 26.331433231219748 26.9260164539022 23.44496303234159 58-59 22.67216799708006 26.592659735695477 27.48175352114874 23.253418746075727 60-61 22.8600619836622 26.965071531462588 27.07473426014167 23.100132224733553 62-63 23.055688140283888 26.513500287915644 26.86864916110358 23.56216241069689 64-65 22.554242130754314 26.52712317273921 27.135964700536192 23.782669995970284 66-67 22.881820761583285 26.30168258809042 27.0217595224186 23.794737127907702 68-69 22.481685964268415 26.40815632064705 27.288241703666266 23.82191601141827 70-71 22.57806896763908 26.180207723325786 27.31185252748623 23.9298707815489 72-73 23.004103515410932 26.41931079730307 27.22539092452661 23.351194762759388 74-75 23.157466573225857 26.59461842470205 26.927335722339578 23.320579279732517 76-77 23.002501844952608 26.440755688647204 26.720562325900815 23.836180140499373 78-79 22.865613447593258 26.538109200667115 26.79696138092737 23.79931597081226 80-81 22.60683628521423 26.85137600116041 27.079839677493954 23.461948036131407 82-83 23.30674026421423 26.244632371864657 27.69655614664644 22.75207121727467 84-85 23.367046421212795 26.588636020206067 26.845385054865933 23.198932503715206 86-87 22.556012388002863 27.096961090624134 27.10914418112939 23.237882340243615 88-89 23.132593689887777 26.216918351781544 27.076806537746744 23.57368142058393 90-91 23.145500122221726 26.237638665284003 27.13435096103059 23.48251025146368 92-93 23.322033582891155 26.43078129742153 27.01407720666009 23.233107913027226 94-95 22.608523544697782 26.403516953447888 27.40366057942133 23.584298922432996 96-97 22.841949668476882 26.340026924707427 27.05715246724521 23.76087093957048 98-99 22.443047928622967 26.727682005727964 26.889897059263042 23.939373006386024 100 23.02552092616102 26.367854029007997 26.36952903274118 24.237096012089804 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2062.0 1 1479.5 2 1903.5 3 2722.5 4 1909.5 5 1417.5 6 2024.5 7 3663.0 8 5799.0 9 6789.0 10 6044.5 11 5311.5 12 5790.0 13 6571.5 14 7376.5 15 8484.5 16 9565.0 17 10452.5 18 11424.5 19 12174.5 20 12921.0 21 13856.5 22 14933.0 23 16907.0 24 19291.0 25 22379.0 26 27169.0 27 32803.0 28 40219.0 29 52644.5 30 70363.0 31 88656.0 32 112503.5 33 138419.0 34 159208.0 35 180834.5 36 204051.5 37 237426.5 38 254410.0 39 249924.5 40 244089.5 41 236224.0 42 238349.5 43 254090.0 44 275253.5 45 311758.5 46 348695.5 47 374975.0 48 398316.0 49 405958.0 50 402370.0 51 411118.0 52 410823.5 53 393823.0 54 375174.0 55 336249.5 56 300814.5 57 259861.0 58 218369.0 59 197723.0 60 164996.0 61 126561.5 62 99866.0 63 80866.0 64 60448.0 65 38965.0 66 26689.5 67 19613.0 68 14876.5 69 11494.5 70 9069.0 71 6985.0 72 5405.5 73 4319.0 74 3402.5 75 2504.0 76 1783.5 77 1185.5 78 764.5 79 534.5 80 405.0 81 294.0 82 210.5 83 163.0 84 120.0 85 90.0 86 81.0 87 69.5 88 54.5 89 43.5 90 33.0 91 29.0 92 23.0 93 17.5 94 17.0 95 10.0 96 8.5 97 7.0 98 6.0 99 4.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05693393988445759 2 0.1319484004704 3 0.016701497936117318 4 0.01623087905587286 5 0.018977979961485866 6 0.05124273482103622 7 0.05621159462640796 8 0.04777328865737358 9 0.039652376816876174 10-11 0.038432051115777174 12-13 0.03951556900285162 14-15 0.018173550015021498 16-17 0.01749498325745972 18-19 0.017144755253556868 20-21 0.020187361037462905 22-23 0.024576155711370538 24-25 0.023804559640272064 26-27 0.018644168895265958 28-29 0.017620846446362307 30-31 0.01591895723989688 32-33 0.022212116685026276 34-35 0.022162865871977435 36-37 0.02225042287295315 38-39 0.020324168851487457 40-41 0.023645862576003582 42-43 0.03060117184101181 44-45 0.039466318189802785 46-47 0.04563361444602959 48-49 0.03868924980614333 50-51 0.027230227303446843 52-53 0.03912156249846092 54-55 0.03618840296577452 56-57 0.03774253973309343 58-59 0.04204377740602534 60-61 0.02274840331600252 62-63 0.02500846840368812 64-65 0.024756742025882943 66-67 0.031531464976378765 68-69 0.03801068304858155 70-71 0.03995335400773019 72-73 0.043822278988344524 74-75 0.026524298983080157 76-77 0.020209250287706832 78-79 0.019355569528193627 80-81 0.023996090579906433 82-83 0.019897328471730855 84-85 0.01242762182599031 86-87 0.014140455657577704 88-89 0.018031269888435963 90-91 0.020444559727829062 92-93 0.01912573240063238 94-95 0.02262254012709993 96-97 0.02377719807746715 98-99 0.026551660545885068 100 0.02842319144174094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9136905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.11839173601802 #Duplication Level Percentage of deduplicated Percentage of total 1 74.351680120201 43.955517515771454 2 14.629072928277745 17.296945282173997 3 5.07430222459805 8.999537601022055 4 2.2197425692777597 5.249104430547109 5 1.2094303709278922 3.574978922297636 6 0.6591720462871857 2.338151475230307 7 0.4228966314951922 1.7500678107202658 8 0.295729886018762 1.3986460199764101 9 0.20197079994121642 1.0746169983145581 >10 0.8764361048342066 8.565217229429702 >50 0.037803891745089066 1.5463606975191226 >100 0.01994309198192745 2.1694526148494293 >500 0.0012959949584224462 0.5242589419214984 >1k 4.2365575133058276E-4 0.49148790407390897 >5k 2.4920926056966698E-5 0.07995518069789143 >10k+ 7.47627781709001E-5 0.9857013754546574 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29665 0.32467230424306703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22760 0.24909966777590442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14480 0.15847817176604112 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1889250243928332E-5 2 0.0 0.0 0.0 0.0 2.1889250243928332E-5 3 0.0 0.0 0.0 0.0 2.1889250243928332E-5 4 0.0 0.0 0.0 0.0 2.1889250243928332E-5 5 0.0 0.0 0.0 0.0 2.1889250243928332E-5 6 0.0 0.0 0.0 0.0 3.28338753658925E-5 7 0.0 0.0 0.0 0.0 3.28338753658925E-5 8 0.0 0.0 0.0 0.0 3.28338753658925E-5 9 0.0 0.0 0.0 0.0 3.28338753658925E-5 10-11 0.0 0.0 0.0 5.472312560982083E-6 3.28338753658925E-5 12-13 0.0 0.0 0.0 2.1889250243928332E-5 4.3778500487856664E-5 14-15 0.0 0.0 0.0 4.3778500487856664E-5 8.208468841473125E-5 16-17 0.0 0.0 0.0 5.472312560982083E-5 9.85016260976775E-5 18-19 0.0 0.0 0.0 9.302931353669542E-5 1.0944625121964166E-4 20-21 0.0 0.0 0.0 1.532247517074983E-4 1.1491856378062373E-4 22-23 0.0 0.0 0.0 4.158957546346383E-4 1.3133550146357E-4 24-25 0.0 0.0 0.0 7.93485321342402E-4 1.4228012658553416E-4 26-27 0.0 0.0 0.0 0.0015705537050018578 1.4228012658553416E-4 28-29 0.0 0.0 0.0 0.0043778500487856664 1.4228012658553416E-4 30-31 0.0 0.0 0.0 0.015245862794896084 1.4775243914651626E-4 32-33 0.0 0.0 0.0 0.04232286534663543 1.641693768294625E-4 34-35 0.0 0.0 0.0 0.0799340695782653 1.641693768294625E-4 36-37 0.0 0.0 0.0 0.14218162495943648 1.641693768294625E-4 38-39 0.0 0.0 0.0 0.23430253461100886 1.641693768294625E-4 40-41 0.0 1.0944625121964166E-5 0.0 0.33833666870783924 1.641693768294625E-4 42-43 0.0 1.0944625121964166E-5 0.0 0.4378999234423473 1.7511400195142665E-4 44-45 0.0 1.0944625121964166E-5 0.0 0.5515379660837012 1.97003252195355E-4 46-47 0.0 1.0944625121964166E-5 0.0 0.6842853241880046 2.188925024392833E-4 48-49 0.0 1.0944625121964166E-5 0.0 0.8318462323948865 2.2436481500026542E-4 50-51 0.0 1.0944625121964166E-5 0.0 0.9873255768775093 2.298371275612475E-4 52-53 0.0 1.0944625121964166E-5 0.0 1.1371520224846379 2.4078175268321165E-4 54-55 0.0 1.0944625121964166E-5 0.0 1.298984721850561 2.4078175268321165E-4 56-57 0.0 1.0944625121964166E-5 0.0 1.4857109710563916 2.4078175268321165E-4 58-59 0.0 1.0944625121964166E-5 0.0 1.7043079686173819 2.4078175268321165E-4 60-61 0.0 1.0944625121964166E-5 0.0 1.9235123928726412 2.4078175268321165E-4 62-63 0.0 1.0944625121964166E-5 0.0 2.145704699786197 2.4078175268321165E-4 64-65 0.0 1.0944625121964166E-5 0.0 2.370074987099023 2.4078175268321165E-4 66-67 0.0 1.0944625121964166E-5 0.0 2.621390941462125 2.4078175268321165E-4 68-69 0.0 1.0944625121964166E-5 0.0 2.9048020089953877 2.4078175268321165E-4 70-71 0.0 1.0944625121964166E-5 0.0 3.2075631737442825 2.4625406524419373E-4 72-73 0.0 1.0944625121964166E-5 0.0 3.5026631009078018 2.845602531710683E-4 74-75 0.0 1.0944625121964166E-5 0.0 3.7916230933778996 2.845602531710683E-4 76-77 0.0 1.0944625121964166E-5 0.0 4.119584257470117 2.9003256573205036E-4 78-79 0.0 1.0944625121964166E-5 0.0 4.471678319956265 3.064495034149967E-4 80-81 0.0 2.1889250243928332E-5 0.0 4.840517658879019 3.1739412853696084E-4 82-83 0.0 2.1889250243928332E-5 0.0 5.220925466555688 3.28338753658925E-4 84-85 0.0 2.1889250243928332E-5 0.0 5.595472427479546 3.28338753658925E-4 86-87 0.0 2.1889250243928332E-5 0.0 5.981615218720124 3.447556913418712E-4 88 0.0 2.1889250243928332E-5 0.0 6.291266024983296 3.502280039028533E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15245 0.0 46.76755 1 GTATCAA 27090 0.0 40.841625 1 GTACTAG 6430 0.0 37.033855 1 TACCTGG 6260 0.0 34.053883 2 CAACGCA 31525 0.0 33.6258 5 ATCAACG 31980 0.0 33.220882 3 TATCAAC 34215 0.0 31.936579 2 GCATCGT 6610 0.0 31.431759 8 TAGGCAT 8120 0.0 30.451654 5 AACGCAG 35845 0.0 30.189472 6 TCAACGC 36330 0.0 29.553701 4 GTACATG 38865 0.0 29.254835 1 CTAGGCA 9055 0.0 28.44941 4 TACATGG 39485 0.0 28.341475 2 GTGGTAT 5580 0.0 28.253353 1 ACATGGG 39310 0.0 27.50464 3 ACGCAGA 38705 0.0 27.314999 7 ACCTGGG 7630 0.0 26.923906 3 CGCAGAG 39245 0.0 26.901302 8 GTACCTG 8155 0.0 26.545643 1 >>END_MODULE