FastQCFastQC Report
Mon 6 Jun 2016
ERR1211198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9136905
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272800.2985693733271825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221170.24206227382248144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145890.1596711359043352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160200.047.8718951
GTATCAA280200.039.251051
GTACTAG67250.034.9596371
TACCTGG61100.033.3817752
CAACGCA331050.031.8223025
ATCAACG335600.031.4971453
TATCAAC363200.030.1100482
AACGCAG370450.028.9070846
TCAACGC377650.028.2762974
TAGGCAT89450.028.2087295
TAGTACT92700.028.1902374
GTACATG379500.027.902671
CTGTGCG94700.027.7857999
TACATGG386050.027.2078882
CTAGGCA98900.026.803194
GTACCTG79250.026.4027941
ACATGGG381350.026.28883
ACGCAGA403350.026.0366447
GTGGTAT57200.025.7298621
GGGCACC89650.025.53646794
Produced by FastQC (version 0.11.5)