##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211198_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9136905 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12508918501396 33.0 31.0 34.0 30.0 34.0 2 32.27070216884164 34.0 31.0 34.0 30.0 34.0 3 32.344076686799305 34.0 31.0 34.0 30.0 34.0 4 35.969453222945845 37.0 35.0 37.0 35.0 37.0 5 35.85021415895208 37.0 35.0 37.0 35.0 37.0 6 35.87746879276954 37.0 35.0 37.0 35.0 37.0 7 35.81469534815126 37.0 35.0 37.0 35.0 37.0 8 35.80246199342118 37.0 35.0 37.0 35.0 37.0 9 37.39773063198096 39.0 37.0 39.0 34.0 39.0 10-11 37.37295539353862 39.0 37.0 39.0 34.0 39.0 12-13 37.26378067846826 39.0 37.0 39.0 33.5 39.0 14-15 38.47367002283596 40.0 38.0 41.0 34.0 41.0 16-17 38.42590625600245 40.0 38.0 41.0 34.0 41.0 18-19 38.44713811733842 40.0 38.0 41.0 34.0 41.0 20-21 38.40277960644222 40.0 38.0 41.0 34.0 41.0 22-23 38.270480649629164 40.0 38.0 41.0 34.0 41.0 24-25 38.20053349575157 40.0 38.0 41.0 33.5 41.0 26-27 38.05494229172789 40.0 37.0 41.0 33.0 41.0 28-29 38.032336606323476 40.0 37.0 41.0 33.0 41.0 30-31 38.04628963527584 40.0 37.0 41.0 33.0 41.0 32-33 37.91400348367418 40.0 37.0 41.0 33.0 41.0 34-35 37.77173408282126 40.0 37.0 41.0 33.0 41.0 36-37 37.625766493139636 40.0 36.5 41.0 33.0 41.0 38-39 37.53336277437491 40.0 36.0 41.0 32.0 41.0 40-41 37.42967345069255 40.0 36.0 41.0 32.0 41.0 42-43 37.28146642654159 39.0 36.0 41.0 32.0 41.0 44-45 37.13144724608607 39.0 36.0 41.0 31.0 41.0 46-47 36.8825551431256 39.0 35.0 41.0 31.0 41.0 48-49 36.63352223756294 39.0 35.0 41.0 30.0 41.0 50-51 36.42083008414775 39.0 35.0 40.0 30.0 41.0 52-53 36.20355787873464 38.5 35.0 40.0 29.5 41.0 54-55 35.95289674129259 38.0 35.0 40.0 29.0 41.0 56-57 35.67955204743838 38.0 34.5 40.0 28.5 41.0 58-59 35.38546887594869 37.5 34.0 40.0 28.0 41.0 60-61 35.106445672796205 37.0 34.0 40.0 28.0 41.0 62-63 34.78625366029307 36.5 34.0 39.5 27.0 41.0 64-65 34.40162571461562 36.0 33.0 39.0 26.0 40.5 66-67 34.08801240682703 35.0 33.0 39.0 26.0 40.0 68-69 33.75762148123462 35.0 33.0 38.0 26.0 40.0 70-71 33.44612853039405 35.0 32.5 37.5 26.0 40.0 72-73 33.117273901829996 35.0 32.0 37.0 25.5 39.0 74-75 32.7935190855109 35.0 32.0 36.5 25.0 39.0 76-77 31.831824343144646 34.0 30.5 35.0 24.5 38.0 78-79 32.34491077667985 34.5 31.5 35.5 25.0 37.5 80-81 32.29441293304461 35.0 32.0 35.0 25.0 37.0 82-83 32.06563234486951 34.5 31.5 35.0 25.0 37.0 84-85 31.83029953797265 34.0 31.0 35.0 24.5 36.0 86-87 31.609151348295732 34.0 31.0 35.0 24.0 36.0 88-89 31.408441261017817 34.0 31.0 35.0 24.0 35.5 90-91 31.20244059667907 34.0 31.0 35.0 23.0 35.0 92-93 31.023701789610378 34.0 31.0 35.0 23.0 35.0 94-95 30.84060204193871 34.0 30.0 35.0 20.5 35.0 96-97 30.67077506004495 34.0 30.0 35.0 20.0 35.0 98-99 30.49687569258956 34.0 30.0 35.0 20.0 35.0 100 28.6830152004426 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2958520344526967 1101 2 -0.3546144173650241 1101 3 -0.3197538142128842 1101 4 -0.21144558010922054 1101 5 -0.27152485857432396 1101 6 -0.31445349938169187 1101 7 -0.33196167886622874 1101 8 -0.301525669476554 1101 9 -0.47643349947344404 1101 10-11 -0.47419868217875916 1101 12-13 -0.46024535595098826 1101 14-15 -0.7324894217682072 1101 16-17 -0.6501836563492063 1101 18-19 -0.7281548160481961 1101 20-21 -0.6721537154378083 1101 22-23 -0.7272273671189424 1101 24-25 -0.7497999687941004 1101 26-27 -0.8462395260371665 1101 28-29 -0.8451768709485421 1101 30-31 -0.6712274926255617 1101 32-33 -0.7101488246092984 1101 34-35 -0.7479076230175679 1101 36-37 -0.7037193621027242 1101 38-39 -0.8149055939361887 1101 40-41 -0.8239951874157398 1101 42-43 -0.8200028603672465 1101 44-45 -0.8431440004445037 1101 46-47 -0.8266415281429289 1101 48-49 -0.7293370127608938 1101 50-51 -0.9048196738519323 1101 52-53 -0.9156710919957334 1101 54-55 -0.938471379296324 1101 56-57 -0.9354701927049476 1101 58-59 -0.8724157799402192 1101 60-61 -0.906842939587456 1101 62-63 -1.020689099109127 1101 64-65 -0.9319834250958934 1101 66-67 -1.0434335784205189 1101 68-69 -1.0974640644855285 1101 70-71 -1.1484774040705048 1101 72-73 -1.2214019147635398 1101 74-75 -1.2076916224342469 1101 76-77 -1.1445828482403613 1101 78-79 -0.9891769307361784 1101 80-81 -0.8860205664576633 1101 82-83 -0.9414682123316709 1101 84-85 -0.8774531108158641 1101 86-87 -1.063887006042851 1101 88-89 -1.130568486267716 1101 90-91 -1.0645546911993193 1101 92-93 -1.1514939828055262 1101 94-95 -1.155969455271542 1101 96-97 -1.0762505332790795 1101 98-99 -1.232040482485953 1101 100 -1.0947847542041131 1102 1 -0.44471471776434157 1102 2 -0.35090123574236287 1102 3 -0.37406631040407134 1102 4 -0.23334538801477578 1102 5 -0.33939456572653626 1102 6 -0.31613354799684146 1102 7 -0.34314496948420015 1102 8 -0.38954578110705995 1102 9 -0.5025815727769043 1102 10-11 -0.5602819338124192 1102 12-13 -0.5531516428021703 1102 14-15 -0.6762459493394175 1102 16-17 -0.7195951584836777 1102 18-19 -0.6820814656045968 1102 20-21 -0.7800495850196896 1102 22-23 -0.6885810639076766 1102 24-25 -0.7249153518870628 1102 26-27 -0.8173547324408403 1102 28-29 -0.8394405145808932 1102 30-31 -0.7105608350493071 1102 32-33 -0.7205230100745368 1102 34-35 -0.7543388292880948 1102 36-37 -0.717212800468225 1102 38-39 -0.8195076571322559 1102 40-41 -0.7384504836205821 1102 42-43 -0.6932669068458779 1102 44-45 -0.7442937267165846 1102 46-47 -0.7936477377300548 1102 48-49 -0.800916283023831 1102 50-51 -0.840405564697285 1102 52-53 -0.8816593376862727 1102 54-55 -0.805528258347131 1102 56-57 -0.891415663024496 1102 58-59 -0.9269818146720183 1102 60-61 -0.9255213231134434 1102 62-63 -0.9232823836481501 1102 64-65 -0.8879843130728418 1102 66-67 -0.9471827642369561 1102 68-69 -1.0335680571060024 1102 70-71 -1.0320180244484902 1102 72-73 -0.9797756551812355 1102 74-75 -1.112265262660344 1102 76-77 -1.0101045221192635 1102 78-79 -0.8053656809679026 1102 80-81 -0.8215939396052185 1102 82-83 -0.7058060988562218 1102 84-85 -0.8341002488443081 1102 86-87 -0.8145279276148827 1102 88-89 -0.8827835120149778 1102 90-91 -0.9388855073574831 1102 92-93 -1.0089208174870556 1102 94-95 -1.1169554097526877 1102 96-97 -1.159715004573961 1102 98-99 -1.1591963069508786 1102 100 -1.2098251034805365 1103 1 -0.4199163827074486 1103 2 -0.3858733740602105 1103 3 -0.38207519128457434 1103 4 -0.23296398156429632 1103 5 -0.2426564112371281 1103 6 -0.34194424730798545 1103 7 -0.4039980605790703 1103 8 -0.3942561642889828 1103 9 -0.5784790230940615 1103 10-11 -0.5500679023667274 1103 12-13 -0.5672760544471771 1103 14-15 -0.7086628081619608 1103 16-17 -0.7100267203875603 1103 18-19 -0.6657237849320552 1103 20-21 -0.7036403459589238 1103 22-23 -0.7229872327909419 1103 24-25 -0.8442372421541151 1103 26-27 -0.8724688539097158 1103 28-29 -0.9036677642368218 1103 30-31 -0.8241756522153381 1103 32-33 -0.7733318872210901 1103 34-35 -0.8142365757029069 1103 36-37 -0.8729572351222785 1103 38-39 -0.8420744876805202 1103 40-41 -0.8489340125980362 1103 42-43 -0.7135883159542402 1103 44-45 -0.7210426826230005 1103 46-47 -0.8552760858530704 1103 48-49 -0.9135354561751114 1103 50-51 -0.9110097927435348 1103 52-53 -0.9611029088915117 1103 54-55 -0.9909653481045027 1103 56-57 -0.9435829027589904 1103 58-59 -0.9346601542051047 1103 60-61 -1.0488097237795628 1103 62-63 -0.9327840403962 1103 64-65 -0.8833918751718457 1103 66-67 -1.0697282526446088 1103 68-69 -1.0243606125381817 1103 70-71 -1.201559168283346 1103 72-73 -1.089547207900793 1103 74-75 -1.0800623659834407 1103 76-77 -1.0314393627311986 1103 78-79 -0.9261410509821815 1103 80-81 -0.8041442351884598 1103 82-83 -0.8330974529285271 1103 84-85 -0.8431301956185919 1103 86-87 -0.8472230823726541 1103 88-89 -0.8903681684018885 1103 90-91 -0.8596130517947884 1103 92-93 -0.9554354252203439 1103 94-95 -0.9998114267329399 1103 96-97 -1.1899153928734236 1103 98-99 -1.2137372443797183 1103 100 -1.2792804518532712 1104 1 -0.3672754481682041 1104 2 -0.34367442422170313 1104 3 -0.42780773959187 1104 4 -0.29360014670564993 1104 5 -0.28690625832562944 1104 6 -0.3572754717871618 1104 7 -0.323645799460067 1104 8 -0.34944485704615147 1104 9 -0.594523498940454 1104 10-11 -0.5538833795569573 1104 12-13 -0.6025527563281585 1104 14-15 -0.7701134968068146 1104 16-17 -0.6950553548128937 1104 18-19 -0.7925876658782869 1104 20-21 -0.8091131736017658 1104 22-23 -0.739207273950413 1104 24-25 -0.7726100616231335 1104 26-27 -0.7700773425099783 1104 28-29 -0.9093017154727008 1104 30-31 -0.8502299389018617 1104 32-33 -0.858756107641895 1104 34-35 -0.8620429161511964 1104 36-37 -0.7966441319386846 1104 38-39 -0.7972085914733711 1104 40-41 -0.8559899641664259 1104 42-43 -0.7831458522262977 1104 44-45 -0.8375788406115632 1104 46-47 -0.9399596221617728 1104 48-49 -0.8827380131536842 1104 50-51 -0.8580424234299002 1104 52-53 -0.8495029858308243 1104 54-55 -0.9738866244507776 1104 56-57 -0.9282515931950499 1104 58-59 -0.9379858835133774 1104 60-61 -0.961239715329512 1104 62-63 -0.949269489285534 1104 64-65 -0.936984993266833 1104 66-67 -0.9988102323219437 1104 68-69 -1.0575760580995635 1104 70-71 -1.0877477859390368 1104 72-73 -1.09038787369699 1104 74-75 -1.0382876487612265 1104 76-77 -0.9245370898392977 1104 78-79 -0.7878292140299905 1104 80-81 -0.7688231403336445 1104 82-83 -0.7992655399151047 1104 84-85 -0.8304318836038149 1104 86-87 -0.9114475767550445 1104 88-89 -0.9660626061104551 1104 90-91 -0.9476918316611744 1104 92-93 -0.9636170127193324 1104 94-95 -1.0666366148170603 1104 96-97 -1.0358735178979899 1104 98-99 -1.154947290915974 1104 100 -1.1804041606140352 1105 1 -0.3919478266239551 1105 2 -0.35099163224080243 1105 3 -0.4196310442294582 1105 4 -0.24983977278736091 1105 5 -0.31676416973874666 1105 6 -0.38837098764333433 1105 7 -0.3696915645003003 1105 8 -0.46065638311683443 1105 9 -0.5707950154282955 1105 10-11 -0.5517846487191704 1105 12-13 -0.5514659431964262 1105 14-15 -0.7873294472094301 1105 16-17 -0.7193333888503304 1105 18-19 -0.7118824840860469 1105 20-21 -0.702780401150406 1105 22-23 -0.7799655513587425 1105 24-25 -0.6891156542368648 1105 26-27 -0.7206908075259761 1105 28-29 -0.7115793881525221 1105 30-31 -0.7629001157213082 1105 32-33 -0.7889486381425854 1105 34-35 -0.8017129882984051 1105 36-37 -0.7318450501253508 1105 38-39 -0.79343985881858 1105 40-41 -0.8220394194138549 1105 42-43 -0.8353168801320692 1105 44-45 -0.8907971088457529 1105 46-47 -0.7886076630170962 1105 48-49 -0.9211850861541109 1105 50-51 -0.9661270296463087 1105 52-53 -0.9970127884935778 1105 54-55 -0.8616791227551133 1105 56-57 -0.9616378075777519 1105 58-59 -1.0229728068631658 1105 60-61 -0.9192184884925751 1105 62-63 -0.9984321128167934 1105 64-65 -0.951893961060648 1105 66-67 -0.9884305241361062 1105 68-69 -0.9677894458488439 1105 70-71 -0.9957102452188948 1105 72-73 -1.0124123369154532 1105 74-75 -0.9993455467554071 1105 76-77 -0.9954118372479925 1105 78-79 -0.8725825264322857 1105 80-81 -0.8721772383907549 1105 82-83 -0.8622751039717009 1105 84-85 -0.9201004770570194 1105 86-87 -0.8973912349940676 1105 88-89 -0.9307740703370975 1105 90-91 -0.9159774497243305 1105 92-93 -0.9663505602334013 1105 94-95 -0.8975126907798092 1105 96-97 -1.0016945702660678 1105 98-99 -1.0223923494867329 1105 100 -1.185042009152287 1106 1 -0.4557912880181867 1106 2 -0.4063417102837832 1106 3 -0.47196386488364084 1106 4 -0.32553486547682553 1106 5 -0.3877295567361827 1106 6 -0.4421506268601121 1106 7 -0.45177241293581716 1106 8 -0.4753188791005769 1106 9 -0.569983205072738 1106 10-11 -0.5456750737871587 1106 12-13 -0.6614798879537176 1106 14-15 -0.8180527745433608 1106 16-17 -0.7529962773983243 1106 18-19 -0.735080392912252 1106 20-21 -0.7961188393666774 1106 22-23 -0.8397565055774052 1106 24-25 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-0.8532588300841937 1106 90-91 -0.8920903928889921 1106 92-93 -0.7863839846914651 1106 94-95 -0.8884045203641868 1106 96-97 -0.874273333155017 1106 98-99 -0.8958093878159517 1106 100 -0.9846023796953567 1107 1 -0.4396163417393453 1107 2 -0.4387738572766544 1107 3 -0.44172958130001305 1107 4 -0.2618787562485636 1107 5 -0.26952116389310987 1107 6 -0.36401339290002 1107 7 -0.43162988784212075 1107 8 -0.4356312494530741 1107 9 -0.6359535356624946 1107 10-11 -0.5916004870676588 1107 12-13 -0.5752798179386573 1107 14-15 -0.7257366650379353 1107 16-17 -0.7333193871107397 1107 18-19 -0.698525123940513 1107 20-21 -0.7103309076486326 1107 22-23 -0.7264447823256006 1107 24-25 -0.761937777142812 1107 26-27 -0.827983555394276 1107 28-29 -0.8292761544123337 1107 30-31 -0.8867176844631928 1107 32-33 -0.7803435043414382 1107 34-35 -0.7989103888799463 1107 36-37 -0.8021026816454864 1107 38-39 -0.8583703767281676 1107 40-41 -0.8805349186235532 1107 42-43 -0.7382433684008518 1107 44-45 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0.9193364286074299 2311 32-33 0.9769555753990247 2311 34-35 1.0104516775330836 2311 36-37 1.0417200851073076 2311 38-39 1.0663132801317943 2311 40-41 1.0527667085927277 2311 42-43 1.0324294028074519 2311 44-45 1.0736785510695412 2311 46-47 0.7638476992987933 2311 48-49 1.0149539649874697 2311 50-51 1.0665161935020535 2311 52-53 1.0254480789078428 2311 54-55 1.0605911332446212 2311 56-57 1.1630180851051648 2311 58-59 1.13468375891415 2311 60-61 1.2231792466352829 2311 62-63 1.1450828845800913 2311 64-65 1.2180289141739706 2311 66-67 1.2316328314309004 2311 68-69 1.2510477298703009 2311 70-71 1.2168136072854097 2311 72-73 1.2380607209545929 2311 74-75 1.2048248529810195 2311 76-77 1.1992690705030071 2311 78-79 0.9477699885031399 2311 80-81 0.9535727752408647 2311 82-83 1.0107185410180008 2311 84-85 1.1157125994918644 2311 86-87 1.1096815847752524 2311 88-89 1.1391527801974313 2311 90-91 1.1572221834654428 2311 92-93 1.2086007310652391 2311 94-95 1.2596029071636572 2311 96-97 1.280995000368275 2311 98-99 1.3291458068232984 2311 100 1.4433550523736827 2312 1 0.2934350423743197 2312 2 0.40053987077996567 2312 3 0.4655608262305222 2312 4 0.2059895145292998 2312 5 0.36661920983684837 2312 6 0.4414247954990316 2312 7 0.3860473766747248 2312 8 0.4544234594230687 2312 9 0.6515580812919808 2312 10-11 0.6555177473243035 2312 12-13 0.6935687548639251 2312 14-15 0.9198534295576266 2312 16-17 0.9105737561251601 2312 18-19 0.8745284561890685 2312 20-21 0.8837335179116295 2312 22-23 0.9623758278598658 2312 24-25 0.9718209352893226 2312 26-27 1.0486363468046491 2312 28-29 1.062699576617895 2312 30-31 1.0783813397720792 2312 32-33 0.9467442125843135 2312 34-35 1.0833178329660456 2312 36-37 1.1247005129815975 2312 38-39 0.9640415296685063 2312 40-41 1.0654923104606837 2312 42-43 1.128422284814235 2312 44-45 0.8621610141864053 2312 46-47 0.9739636562565934 2312 48-49 1.1494764099026753 2312 50-51 1.003660426068798 2312 52-53 1.1932834770257585 2312 54-55 1.0173431730429456 2312 56-57 1.0260252767904277 2312 58-59 1.2244809182415821 2312 60-61 1.2520030745696147 2312 62-63 1.2825281877970625 2312 64-65 1.2973493366738182 2312 66-67 1.2736996744665916 2312 68-69 1.368066204171292 2312 70-71 1.2507826907635504 2312 72-73 1.306944861689857 2312 74-75 1.3497227992843222 2312 76-77 1.2964917562018314 2312 78-79 1.1598731193125573 2312 80-81 1.1130131433134878 2312 82-83 1.1241080246039274 2312 84-85 1.189413897298465 2312 86-87 1.1903375221535555 2312 88-89 1.1727129261912275 2312 90-91 1.2639319859023317 2312 92-93 1.338456625348364 2312 94-95 1.3262708064756161 2312 96-97 0.9854882591467202 2312 98-99 0.7949099697177324 2312 100 0.9999729931051924 2313 1 0.048949802722361824 2313 2 0.22142312131576602 2313 3 0.2613183989952361 2313 4 0.173473786289577 2313 5 0.3245702424192416 2313 6 0.24956743112732482 2313 7 0.2689522368736377 2313 8 0.40032646285019524 2313 9 0.578455099593036 2313 10-11 0.3560213225976341 2313 12-13 0.40489135908511287 2313 14-15 0.4424593791284934 2313 16-17 0.7295360379791589 2313 18-19 0.7305804951812718 2313 20-21 0.6982927568327213 2313 22-23 0.6321735335615415 2313 24-25 0.5326703343076034 2313 26-27 0.7194346599077335 2313 28-29 0.5830327091301655 2313 30-31 0.8049988546608233 2313 32-33 0.8968558164203202 2313 34-35 0.91330099362618 2313 36-37 0.9547503396499764 2313 38-39 0.9540934797727019 2313 40-41 0.904511184348344 2313 42-43 0.9922507902117133 2313 44-45 0.8484950997756826 2313 46-47 0.8500315469440949 2313 48-49 0.9618603616661119 2313 50-51 0.9102292500804126 2313 52-53 0.9918990462547015 2313 54-55 0.9497611244705837 2313 56-57 0.6055041214438077 2313 58-59 0.7028570577365088 2313 60-61 0.6054131643465013 2313 62-63 0.9398266342321548 2313 64-65 1.0236304351321053 2313 66-67 0.9371258381587992 2313 68-69 1.009112341987212 2313 70-71 1.0168053720210182 2313 72-73 1.0268081222996273 2313 74-75 1.0550497837091655 2313 76-77 1.0288869918044412 2313 78-79 1.0042610759747177 2313 80-81 0.8605427973415942 2313 82-83 0.9281463797806637 2313 84-85 0.9140030371588068 2313 86-87 0.9366264476950334 2313 88-89 0.9373325348864547 2313 90-91 0.8076099495767615 2313 92-93 1.0184974397600755 2313 94-95 1.1237765101229904 2313 96-97 1.0969180228033721 2313 98-99 1.1289542013796385 2313 100 1.2404916052670494 2314 1 -0.4036247886311415 2314 2 0.014732305006020852 2314 3 0.2916917771772418 2314 4 0.24143340411108483 2314 5 0.33285629836762354 2314 6 0.4112366110828347 2314 7 0.4447933824813788 2314 8 0.4521054415133037 2314 9 0.4733710304611023 2314 10-11 0.5759617131812931 2314 12-13 0.4763474367937448 2314 14-15 0.7982965345969717 2314 16-17 0.8119701279951386 2314 18-19 0.839732419940276 2314 20-21 0.8653895561839917 2314 22-23 0.9236970787201528 2314 24-25 0.8678750593104567 2314 26-27 0.7627040809698116 2314 28-29 0.8048060007627882 2314 30-31 0.8399009930014643 2314 32-33 0.8895556655040551 2314 34-35 0.9071380753512557 2314 36-37 0.8979681419815719 2314 38-39 0.6526691847441128 2314 40-41 0.6565580098236765 2314 42-43 0.8380680524719963 2314 44-45 0.9175752943723694 2314 46-47 0.8684144360427979 2314 48-49 0.8604603859366762 2314 50-51 0.877306702525992 2314 52-53 0.8675862112087174 2314 54-55 0.813505946656143 2314 56-57 0.7484229549011374 2314 58-59 0.8852638648407449 2314 60-61 0.955532942862547 2314 62-63 0.8735532772811112 2314 64-65 0.9276093006096602 2314 66-67 0.6363562512401444 2314 68-69 0.6200510533886359 2314 70-71 0.7392596709343593 2314 72-73 0.9049511870794404 2314 74-75 0.9773353086852126 2314 76-77 0.8227326818824565 2314 78-79 0.7066348553181925 2314 80-81 0.7311004841431838 2314 82-83 0.7371390334905641 2314 84-85 0.7775560422454504 2314 86-87 0.8548738233780853 2314 88-89 0.8490042588572244 2314 90-91 0.9241118854732591 2314 92-93 0.8415724156233182 2314 94-95 0.8970228295441274 2314 96-97 0.939803860878321 2314 98-99 1.045082466036785 2314 100 1.0850051092634985 2315 1 0.4285886424029641 2315 2 0.45699906095009624 2315 3 0.4593833759773176 2315 4 0.3331879450181958 2315 5 0.3822860690365175 2315 6 0.4393810168326411 2315 7 0.3991330380565188 2315 8 0.3056810327488293 2315 9 0.564152236581215 2315 10-11 0.6078001247760767 2315 12-13 0.4167999207078381 2315 14-15 0.6619823857738751 2315 16-17 0.8010986988137176 2315 18-19 0.7932168320545117 2315 20-21 0.582375287017733 2315 22-23 0.8680976659018427 2315 24-25 0.9423046299400184 2315 26-27 0.9049856650094199 2315 28-29 0.8766107499300375 2315 30-31 0.8849361990554598 2315 32-33 0.8741814853215573 2315 34-35 0.9296729242865496 2315 36-37 0.8804215842842709 2315 38-39 0.9126322434057457 2315 40-41 0.9550805082278799 2315 42-43 1.0658117701658014 2315 44-45 1.0255240982441478 2315 46-47 1.0448773318777853 2315 48-49 0.9252810376975731 2315 50-51 0.9753527960288366 2315 52-53 1.068458235677447 2315 54-55 0.8873781197856729 2315 56-57 0.867549563971842 2315 58-59 0.8734169951952282 2315 60-61 0.9728056634466569 2315 62-63 1.0607958112912357 2315 64-65 1.1002609374156407 2315 66-67 1.0888276427472903 2315 68-69 1.0940319199183577 2315 70-71 0.9763446552940067 2315 72-73 1.1078420810738834 2315 74-75 0.5054771793649522 2315 76-77 0.5199884784378526 2315 78-79 0.8061113517876066 2315 80-81 0.8412418304845488 2315 82-83 0.8095570466613466 2315 84-85 0.8699488221379781 2315 86-87 0.9212763960233445 2315 88-89 0.9141990894381244 2315 90-91 0.9794503842554647 2315 92-93 0.9636886485200904 2315 94-95 1.0221388306760417 2315 96-97 1.0850717244333197 2315 98-99 1.1677394698420933 2315 100 1.0873780009119862 2316 1 0.263346999846668 2316 2 0.06716902172978934 2316 3 0.2488983203359041 2316 4 0.02273569507865858 2316 5 0.30288763395543583 2316 6 0.3882890709328919 2316 7 0.455732050624043 2316 8 0.4485809477539533 2316 9 0.6446393897115144 2316 10-11 0.6537418603629064 2316 12-13 0.42449941255427603 2316 14-15 0.5816493189148986 2316 16-17 0.8143334959037247 2316 18-19 0.7920450519066407 2316 20-21 0.7411366532798453 2316 22-23 0.8142416468316043 2316 24-25 0.5299053500511306 2316 26-27 0.7831800561725473 2316 28-29 0.9520877477333514 2316 30-31 0.5997255888888944 2316 32-33 0.7331607433651968 2316 34-35 0.8659303092737645 2316 36-37 0.8947145113851036 2316 38-39 0.821152163500436 2316 40-41 0.8460823480617563 2316 42-43 0.7869446394472561 2316 44-45 0.6376695845982141 2316 46-47 0.7535113752765739 2316 48-49 0.8892470145647522 2316 50-51 0.8974464866378824 2316 52-53 0.8720177722141074 2316 54-55 0.822073415033465 2316 56-57 0.9257593459978679 2316 58-59 0.7799656916061366 2316 60-61 0.911184523116404 2316 62-63 0.935591954478916 2316 64-65 0.9307576070974193 2316 66-67 0.9241193077364471 2316 68-69 0.9148753927109396 2316 70-71 0.9333240753277678 2316 72-73 0.9867502217849804 2316 74-75 0.965888423405076 2316 76-77 0.7993701823981283 2316 78-79 0.5662194316517244 2316 80-81 0.5989257643042336 2316 82-83 0.490129865946237 2316 84-85 0.617186026027305 2316 86-87 0.6566070678221365 2316 88-89 0.6588701808005055 2316 90-91 0.616604176819397 2316 92-93 0.3565569726674056 2316 94-95 0.381072360154846 2316 96-97 0.6268527827776467 2316 98-99 0.8146864528840645 2316 100 0.4312272805446469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 12.0 12 41.0 13 219.0 14 861.0 15 2376.0 16 5572.0 17 11233.0 18 19229.0 19 27895.0 20 40164.0 21 56274.0 22 69775.0 23 72673.0 24 74788.0 25 80625.0 26 95316.0 27 116775.0 28 144429.0 29 177907.0 30 220044.0 31 275045.0 32 349909.0 33 462281.0 34 662151.0 35 920504.0 36 1431010.0 37 2060986.0 38 1545139.0 39 213660.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44149811428349 20.291216297005494 13.601981327223825 23.66530426148719 2 15.634878550231177 19.770918051572167 40.70627854837059 23.88792484982606 3 19.09431038190722 25.669545650304997 29.124096179176647 26.112047788611132 4 12.861690008051118 16.055917287834838 37.772404039616674 33.30998866449737 5 13.418263624279774 37.85483158684478 34.08623598472349 14.640668804151952 6 31.437089473952067 36.66903617800557 17.430880588120377 14.462993759921986 7 28.147537919084353 31.820203606855074 22.009923798466513 18.022334675594056 8 26.842218453622973 35.57067737926574 19.23301161607787 18.354092551033418 9 26.794436124429094 15.593348200632798 20.72567511980713 36.88654055513098 10-11 26.334067920702214 25.936705605762057 26.770885736595623 20.958340736940105 12-13 26.118641925247115 23.35243170417116 27.662955891519065 22.865970479062657 14-15 23.37099747780838 24.720274228827186 25.28130727123383 26.627421022130605 16-17 22.445271701689627 27.48784282260702 26.354070393672945 23.712815082030406 18-19 22.104503658514563 27.03278626624661 28.675902835807094 22.186807239431733 20-21 23.228253987537357 26.31704061714552 28.308004734644825 22.146700660672295 22-23 23.39958114919658 25.945809877633618 28.145099462016955 22.50950951115285 24-25 22.882043129096598 25.92290032623942 28.671599877769665 22.52345666689432 26-27 22.78990563608224 26.269350053590617 27.992518437130652 22.948225873196495 28-29 22.318783778592472 26.668962488216724 28.38856310939095 22.623690623799853 30-31 23.165219625963136 26.229577813329175 28.282388387133068 22.322814173574617 32-33 23.255576698930504 26.388811048576148 27.752943677376813 22.60266857511653 34-35 23.166456183157436 26.363004793681643 28.22823608542711 22.24230293773381 36-37 22.97860751802322 26.175732561131156 27.980605536426882 22.86505438441874 38-39 23.06301819982312 26.268477413304996 28.300903931955673 22.36760045491621 40-41 22.78410910786153 25.609323445911304 28.762661649297428 22.843905796929743 42-43 22.849397183234245 25.85635879137311 28.07612211913289 23.218121906259757 44-45 22.689361831614548 26.46669248424019 28.12367281764153 22.720272866503734 46-47 22.766561582914015 26.255260801206052 27.59672884812276 23.38144876775717 48-49 23.3787331946352 26.282785821925454 27.503712118753654 22.8347688646857 50-51 23.016044543786254 26.244119427846783 27.531582925597064 23.2082531027699 52-53 22.701829608881987 26.632022703433172 27.27179637839216 23.39435130929268 54-55 22.65978230899662 26.488562744849293 27.33951716384575 23.51213778230834 56-57 23.308162052904784 26.239204725295362 26.984684467368908 23.46794875443095 58-59 22.55754474583575 26.598709004726786 27.540573937792807 23.303172311644662 60-61 22.845754925893583 26.953202321307323 27.098345595428093 23.102697157371 62-63 23.067585085850478 26.509769052828364 26.826910095797906 23.59573576552325 64-65 22.49506126862566 26.540165146075157 27.063443216673598 23.901330368625583 66-67 22.749492766550397 26.31951606090503 27.07628964111406 23.854701531430514 68-69 22.42905536912081 26.407931853423467 27.27938310240302 23.8836296750527 70-71 22.435984231305145 26.11469299461917 27.372820261440232 24.07650251263545 72-73 22.78404975157772 26.407460283942306 27.22803041870642 23.580459545773554 74-75 22.933303801620465 26.64544777331907 26.89194195901676 23.52930646604371 76-77 22.762407984464616 26.457311223603053 26.719102692006093 24.061178099926238 78-79 22.584398146422586 26.5332139969866 26.833837163245217 24.048550693345597 80-81 22.29536717653131 26.918061282697973 27.038163454091823 23.74840808667889 82-83 23.000860299465234 26.23045679082296 27.659440753340945 23.109242156370854 84-85 23.091830304710687 26.607289445283882 26.774030547009257 23.526849702996177 86-87 22.159400211506853 27.109250834928616 27.10147017741323 23.629878776151305 88-89 22.637418217490957 26.24064026516935 27.06509426078644 24.05684725655325 90-91 22.669990049779575 26.214735546092122 27.13839675855172 23.976877645576586 92-93 22.797379787205156 26.51046105549536 26.916841922703988 23.775317234595498 94-95 22.018376440869076 26.40288629718143 27.361486271633357 24.217250990316142 96-97 22.223567852635266 26.37346285971588 27.079745530283063 24.3232237573658 98-99 21.754937043979503 26.754171296818818 26.962981078327452 24.52791058087423 100 22.07546004774223 26.43181799271561 26.64087088621786 24.851851073324305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1928.0 1 1407.0 2 1656.0 3 2253.0 4 1597.0 5 1228.5 6 1857.0 7 3475.0 8 5343.0 9 6075.0 10 5375.5 11 4671.0 12 4969.0 13 5716.0 14 6677.5 15 7832.0 16 8978.5 17 9980.0 18 11052.0 19 12092.0 20 13192.0 21 14242.5 22 15594.0 23 17646.5 24 20056.5 25 23390.0 26 28155.5 27 33781.5 28 40813.5 29 53090.0 30 70745.0 31 88920.0 32 112968.5 33 138874.5 34 159297.5 35 181313.0 36 204823.0 37 238501.5 38 255902.5 39 250393.0 40 243196.5 41 235662.0 42 238556.0 43 253618.0 44 276983.5 45 315931.5 46 351752.0 47 378499.5 48 404248.0 49 411385.0 50 405986.5 51 411370.0 52 410508.0 53 392472.0 54 375598.5 55 339683.5 56 301227.0 57 260140.5 58 218900.5 59 199176.5 60 165365.0 61 123198.5 62 95387.5 63 76841.0 64 56355.0 65 35175.5 66 23899.5 67 17491.5 68 13147.0 69 9979.0 70 7730.5 71 5886.0 72 4594.0 73 3831.5 74 2911.5 75 1950.5 76 1329.5 77 804.0 78 461.0 79 282.5 80 171.0 81 109.0 82 74.0 83 43.0 84 28.5 85 24.0 86 14.0 87 8.0 88 6.0 89 3.5 90 1.5 91 1.5 92 0.0 93 0.0 94 1.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04418345161736934 2 0.0 3 0.0 4 0.030327556212962705 5 0.0 6 0.0 7 8.865146348790975E-4 8 0.0 9 0.010627230993427205 10-11 3.228664410979429E-4 12-13 0.0 14-15 1.0944625121964166E-4 16-17 2.0247556475633708E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.238436319519575E-4 26-27 4.815635053664233E-4 28-29 1.7511400195142665E-4 30-31 0.0023202605258564033 32-33 0.015245862794896082 34-35 0.026059152415396677 36-37 0.031246904723207694 38-39 0.02228325674831904 40-41 0.0012203257010990046 42-43 8.208468841473125E-5 44-45 0.011043126748061844 46-47 0.018080520701484804 48-49 0.003026188846223092 50-51 0.010895374308915328 52-53 0.01114162837415952 54-55 0.010156612113182747 56-57 4.0495112951267415E-4 58-59 0.008487556782083211 60-61 0.004547491738176111 62-63 0.0012969380769527537 64-65 0.035389445331871126 66-67 0.06028299517177863 68-69 0.05987257172970497 70-71 0.05210188789311042 72-73 0.04563361444602959 74-75 0.03525263751784658 76-77 0.03193094379333046 78-79 0.015119999605993495 80-81 0.0011054071373183807 82-83 0.0378410413591911 84-85 0.05235361427091559 86-87 0.05785328839470258 88-89 0.07046149653520531 90-91 0.048637914042008755 92-93 0.03941159506419296 94-95 0.012980325394649501 96-97 0.0036828663535409417 98-99 0.02228325674831904 100 0.031225015472963767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9136905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.384199697891034 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7900147121981 41.76996740257203 2 15.308303610609586 17.569095028543337 3 5.480224729413658 9.434349307859828 4 2.459864219533055 5.646293584135268 5 1.2733557476323143 3.653525025429504 6 0.7173471602680705 2.4698635618522804 7 0.45576288843564633 1.8307512023415133 8 0.3158640150953021 1.4500482975685187 9 0.2089515320937802 1.0791464800364794 >10 0.9267659161063041 8.907257159505072 >50 0.03991786614493802 1.58601938606884 >100 0.021327250059225696 2.3062713592960375 >500 0.0015508501131417482 0.5874844149399099 >1k 6.461226706049519E-4 0.679729482781666 >5k 2.584490658635381E-5 0.08122879990528999 >10k+ 7.753471975906144E-5 0.9489695071644851 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27280 0.2985693733271825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22117 0.24206227382248144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14589 0.1596711359043352 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0944625121964166E-5 2 0.0 0.0 0.0 0.0 1.0944625121964166E-5 3 0.0 0.0 0.0 0.0 1.0944625121964166E-5 4 0.0 0.0 0.0 0.0 1.0944625121964166E-5 5 0.0 0.0 0.0 0.0 1.0944625121964166E-5 6 0.0 0.0 0.0 0.0 2.1889250243928332E-5 7 0.0 0.0 0.0 0.0 2.1889250243928332E-5 8 0.0 0.0 0.0 0.0 3.28338753658925E-5 9 0.0 0.0 0.0 0.0 4.3778500487856664E-5 10-11 0.0 0.0 0.0 1.0944625121964166E-5 4.3778500487856664E-5 12-13 0.0 0.0 0.0 3.830618792687458E-5 5.472312560982083E-5 14-15 0.0 0.0 0.0 6.5667750731785E-5 1.0397393865865958E-4 16-17 0.0 0.0 0.0 9.85016260976775E-5 1.4228012658553416E-4 18-19 0.0 0.0 0.0 1.3133550146357E-4 1.4228012658553416E-4 20-21 5.472312560982083E-6 0.0 0.0 2.0794787731731915E-4 1.4228012658553416E-4 22-23 1.0944625121964166E-5 0.0 0.0 4.7061888024445915E-4 1.6416937682946247E-4 24-25 1.0944625121964166E-5 0.0 0.0 8.700976971961513E-4 1.97003252195355E-4 26-27 1.0944625121964166E-5 0.0 0.0 0.001641693768294625 1.97003252195355E-4 28-29 1.0944625121964166E-5 0.0 0.0 0.004448990112078433 1.97003252195355E-4 30-31 1.0944625121964166E-5 0.0 0.0 0.015333419795871796 1.97003252195355E-4 32-33 1.0944625121964166E-5 0.0 0.0 0.04237211615968427 2.298371275612475E-4 34-35 1.0944625121964166E-5 0.0 0.0 0.07987387414009448 2.298371275612475E-4 36-37 1.0944625121964166E-5 0.0 0.0 0.14189159239370444 2.298371275612475E-4 38-39 1.0944625121964166E-5 0.0 0.0 0.23366227404137396 2.298371275612475E-4 40-41 1.0944625121964166E-5 0.0 0.0 0.33761979576235057 2.4078175268321165E-4 42-43 1.0944625121964166E-5 0.0 0.0 0.4375934739389323 2.4078175268321165E-4 44-45 1.0944625121964166E-5 0.0 0.0 0.5512643504556521 2.6814331548812205E-4 46-47 1.0944625121964166E-5 0.0 0.0 0.6846793306923953 2.7908794061008626E-4 48-49 1.0944625121964166E-5 0.0 0.0 0.8323332682128138 2.9550487829303247E-4 50-51 1.0944625121964166E-5 0.0 0.0 0.9877250556944611 2.9550487829303247E-4 52-53 1.0944625121964166E-5 0.0 0.0 1.1372505241107356 3.2833875365892494E-4 54-55 1.0944625121964166E-5 0.0 0.0 1.2985469368456823 3.6117262902481747E-4 56-57 1.0944625121964166E-5 0.0 0.0 1.4846274531693173 3.6117262902481747E-4 58-59 1.0944625121964166E-5 0.0 0.0 1.7025732455355507 3.6117262902481747E-4 60-61 1.0944625121964166E-5 0.0 0.0 1.9213070509105654 3.6117262902481747E-4 62-63 1.0944625121964166E-5 0.0 0.0 2.142870041879608 3.721172541467816E-4 64-65 1.0944625121964166E-5 0.0 0.0 2.366676680998653 3.721172541467816E-4 66-67 1.0944625121964166E-5 0.0 0.0 2.6174016256051695 3.721172541467816E-4 68-69 1.0944625121964166E-5 0.0 0.0 2.9000684586301375 3.721172541467816E-4 70-71 1.0944625121964166E-5 0.0 0.0 3.2025395908133003 3.721172541467816E-4 72-73 1.0944625121964166E-5 0.0 0.0 3.497365902348771 4.59674255122495E-4 74-75 1.0944625121964166E-5 0.0 0.0 3.786024917628015 4.59674255122495E-4 76-77 1.0944625121964166E-5 0.0 0.0 4.1138711631564515 4.59674255122495E-4 78-79 1.0944625121964166E-5 0.0 0.0 4.466009004143087 4.7061888024445915E-4 80-81 1.0944625121964166E-5 0.0 0.0 4.834498115061939 4.760911928054412E-4 82-83 1.0944625121964166E-5 0.0 0.0 5.214265662168973 4.815635053664233E-4 84-85 1.0944625121964166E-5 0.0 0.0 5.587335098701366 4.815635053664233E-4 86-87 1.0944625121964166E-5 0.0 0.0 5.972367010492064 4.925081304883875E-4 88 1.0944625121964166E-5 0.0 0.0 6.281656644126211 5.034527556103517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16020 0.0 47.871895 1 GTATCAA 28020 0.0 39.25105 1 GTACTAG 6725 0.0 34.959637 1 TACCTGG 6110 0.0 33.381775 2 CAACGCA 33105 0.0 31.822302 5 ATCAACG 33560 0.0 31.497145 3 TATCAAC 36320 0.0 30.110048 2 AACGCAG 37045 0.0 28.907084 6 TCAACGC 37765 0.0 28.276297 4 TAGGCAT 8945 0.0 28.208729 5 TAGTACT 9270 0.0 28.190237 4 GTACATG 37950 0.0 27.90267 1 CTGTGCG 9470 0.0 27.785799 9 TACATGG 38605 0.0 27.207888 2 CTAGGCA 9890 0.0 26.80319 4 GTACCTG 7925 0.0 26.402794 1 ACATGGG 38135 0.0 26.2888 3 ACGCAGA 40335 0.0 26.036644 7 GTGGTAT 5720 0.0 25.729862 1 GGGCACC 8965 0.0 25.536467 94 >>END_MODULE