FastQCFastQC Report
Mon 6 Jun 2016
ERR1211197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11279495
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411450.36477696918168767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311810.27643968103181926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196620.17431631469316666No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC152860.13552025157154643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC136300.038.75735594
GGTATCA281700.038.5210081
GTACTAG140700.038.1607321
GCATCGT139100.036.327088
GTATCAA463750.035.9917071
TAGGCAT169700.034.0709275
TACCTGG48550.032.6637342
ACCTGGG47650.032.2586023
TACCCCG6450.032.0666545
CAACGCA503100.031.2164025
CTAGGCA192500.030.8657634
TATTCTC55450.030.6031655
ATCAACG513950.030.5939753
CTGTGCG191600.030.3961899
TAGTACT197550.030.0053544
ACTGTGC200350.029.7729198
TATCAAC568250.029.248692
CATCGTC180050.028.6911499
GTACTGT208900.028.352726
AAGCTAA70850.028.2626639
Produced by FastQC (version 0.11.5)