##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11279495 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.555508912411415 31.0 31.0 34.0 30.0 34.0 2 31.577015637668175 31.0 31.0 34.0 30.0 34.0 3 31.63970089086435 33.0 31.0 34.0 28.0 34.0 4 35.246272727635414 37.0 35.0 37.0 32.0 37.0 5 35.31101862273089 37.0 35.0 37.0 33.0 37.0 6 35.374643900280994 37.0 35.0 37.0 33.0 37.0 7 35.29113360128268 37.0 35.0 37.0 32.0 37.0 8 35.30717137602348 37.0 35.0 37.0 32.0 37.0 9 36.87338848060131 39.0 37.0 39.0 33.0 39.0 10-11 36.83519226702968 39.0 37.0 39.0 32.5 39.0 12-13 36.706981429576416 39.0 37.0 39.0 32.0 39.0 14-15 37.884033815343685 40.0 37.5 41.0 32.0 41.0 16-17 37.87047496363977 40.0 37.5 41.0 32.0 41.0 18-19 37.895180059036335 40.0 38.0 41.0 32.0 41.0 20-21 37.867099502238354 40.0 37.5 41.0 32.0 41.0 22-23 37.74200839665251 40.0 37.0 41.0 32.0 41.0 24-25 37.633414350553814 40.0 37.0 41.0 32.0 41.0 26-27 37.470217682617886 40.0 37.0 41.0 31.5 41.0 28-29 37.36348590960854 40.0 36.0 41.0 31.0 41.0 30-31 37.21475460559183 39.5 36.0 41.0 31.0 41.0 32-33 37.03628602167029 39.0 36.0 41.0 31.0 41.0 34-35 36.891125976827865 39.0 35.0 41.0 30.0 41.0 36-37 36.78307144956401 39.0 35.0 41.0 30.0 41.0 38-39 36.62258345785871 39.0 35.0 41.0 30.0 41.0 40-41 36.444246661752146 39.0 35.0 40.0 30.0 41.0 42-43 36.220073992674315 39.0 35.0 40.0 29.0 41.0 44-45 35.94133904044463 38.0 35.0 40.0 28.0 41.0 46-47 35.65316634299674 38.0 34.0 40.0 27.0 41.0 48-49 35.38983837485632 38.0 34.0 40.0 26.5 41.0 50-51 34.6558336609928 37.5 33.5 39.5 25.5 40.5 52-53 34.72824594540802 37.5 33.5 39.5 26.0 40.5 54-55 35.16362124368157 38.0 34.0 40.0 26.5 41.0 56-57 35.06360843282434 37.0 34.0 40.0 26.0 41.0 58-59 34.79287849323042 37.0 34.0 40.0 26.0 41.0 60-61 34.556490782610396 36.0 33.5 40.0 26.0 41.0 62-63 34.23150708431539 36.0 33.0 39.0 26.0 41.0 64-65 33.97029193239591 35.0 33.0 39.0 26.0 41.0 66-67 33.804952083404444 35.0 33.0 39.0 25.5 41.0 68-69 33.52776631400608 35.0 33.0 38.0 25.0 41.0 70-71 33.296954695223505 35.0 33.0 37.5 25.0 40.0 72-73 33.023333846063146 35.0 32.5 37.0 24.5 40.0 74-75 32.727577254123524 35.0 32.0 36.5 24.0 39.0 76-77 32.4593595723922 35.0 32.0 36.0 23.5 39.0 78-79 32.1627771012798 35.0 32.0 36.0 23.0 39.0 80-81 31.91658327788611 35.0 31.0 35.0 23.0 37.5 82-83 31.653562947631965 35.0 31.0 35.0 21.5 37.0 84-85 31.445172368089175 35.0 31.0 35.0 20.0 37.0 86-87 31.24917033076392 34.0 31.0 35.0 20.0 36.0 88-89 31.078453778294154 34.0 31.0 35.0 20.0 36.0 90-91 30.858531698449266 34.0 30.5 35.0 19.5 36.0 92-93 30.660486129919825 34.0 30.0 35.0 19.0 35.5 94-95 30.521480970557636 34.0 30.0 35.0 19.0 35.0 96-97 30.34376574483166 34.0 29.5 35.0 18.0 35.0 98-99 30.11969990677774 34.0 29.0 35.0 18.0 35.0 100 28.933881614380784 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4804960013082642 1101 2 -0.42555473542137534 1101 3 -0.4542657066863711 1101 4 -0.39212740134394863 1101 5 -0.2674535057532452 1101 6 -0.2945397401315546 1101 7 -0.36252881811783766 1101 8 -0.36595644406489214 1101 9 -0.4906423164470297 1101 10-11 -0.520595651837084 1101 12-13 -0.48727364360625103 1101 14-15 -0.645702434136858 1101 16-17 -0.6535085209469784 1101 18-19 -0.6686549272369433 1101 20-21 -0.6970612100953275 1101 22-23 -0.7547603264856377 1101 24-25 -0.8616925175179588 1101 26-27 -0.8319569451152731 1101 28-29 -0.77591685285784 1101 30-31 -0.8302568277928231 1101 32-33 -0.8711749158430209 1101 34-35 -0.8732743451825655 1101 36-37 -0.8993792895070953 1101 38-39 -0.9301516389858975 1101 40-41 -0.9592923604313697 1101 42-43 -1.0774513838964523 1101 44-45 -1.022134053646667 1101 46-47 -1.0923098056233655 1101 48-49 -1.0864354629344817 1101 50-51 -1.162093140677598 1101 52-53 -1.0279166590165048 1101 54-55 -0.9505979598147647 1101 56-57 -0.847557808474015 1101 58-59 -0.853079266702089 1101 60-61 -0.7930473087219951 1101 62-63 -0.8047167280374765 1101 64-65 -0.7832452935138079 1101 66-67 -0.8623338936590841 1101 68-69 -0.8834670934635156 1101 70-71 -0.9255068014836709 1101 72-73 -0.9011722339354264 1101 74-75 -0.9276669772965924 1101 76-77 -0.964904077576886 1101 78-79 -1.0538369377582306 1101 80-81 -1.1359488790564711 1101 82-83 -1.0376774726557976 1101 84-85 -1.1650665125881723 1101 86-87 -1.1676831952900422 1101 88-89 -1.3073611750441998 1101 90-91 -1.3325611989595565 1101 92-93 -1.4133697144483186 1101 94-95 -1.522368013607057 1101 96-97 -1.5870826032014058 1101 98-99 -1.6034727017018184 1101 100 -1.742920772844652 1102 1 -0.45310827132223963 1102 2 -0.48608426612356936 1102 3 -0.4904095379091764 1102 4 -0.3824233180971177 1102 5 -0.3852943848052348 1102 6 -0.4389952181713923 1102 7 -0.35917488122224484 1102 8 -0.47971003876665463 1102 9 -0.6167581057586915 1102 10-11 -0.617167517535357 1102 12-13 -0.648798881302433 1102 14-15 -0.7367304978721805 1102 16-17 -0.7799098041814503 1102 18-19 -0.7831451425416844 1102 20-21 -0.8617832687953282 1102 22-23 -0.8554000809830953 1102 24-25 -0.8743813542012475 1102 26-27 -0.8540042568427566 1102 28-29 -0.92801906387146 1102 30-31 -0.9393293575441959 1102 32-33 -1.075416132638118 1102 34-35 -1.029462957846036 1102 36-37 -0.9810763611930398 1102 38-39 -1.0683795324258938 1102 40-41 -1.153431410433754 1102 42-43 -1.0550472322007352 1102 44-45 -1.178775796958682 1102 46-47 -1.2075339982013702 1102 48-49 -1.2070093683672027 1102 50-51 -1.1959824406821866 1102 52-53 -1.1138952997863996 1102 54-55 -0.8844282648990074 1102 56-57 -0.8841738843865556 1102 58-59 -0.9254477272439487 1102 60-61 -0.9245749673073931 1102 62-63 -0.9451533190761907 1102 64-65 -0.9581519584482834 1102 66-67 -0.9861840583561801 1102 68-69 -1.029073665250678 1102 70-71 -1.074145778212376 1102 72-73 -1.0983180046847068 1102 74-75 -1.2083424869794683 1102 76-77 -1.2136865260355592 1102 78-79 -1.1196028282755393 1102 80-81 -1.1996242297673945 1102 82-83 -1.2632290704374327 1102 84-85 -1.3001979218122237 1102 86-87 -1.35617483339324 1102 88-89 -1.3366784927768123 1102 90-91 -1.3627299265120705 1102 92-93 -1.425644994087076 1102 94-95 -1.4896096674676507 1102 96-97 -1.523062584244439 1102 98-99 -1.5550874531344014 1102 100 -1.620567023816328 1103 1 -0.541661658509689 1103 2 -0.40706084430924605 1103 3 -0.5301369959897855 1103 4 -0.42249752580359967 1103 5 -0.37684308566903013 1103 6 -0.43873557141278496 1103 7 -0.4528568466134999 1103 8 -0.42905869091524096 1103 9 -0.5870478209247025 1103 10-11 -0.6128506337019886 1103 12-13 -0.6208949736461022 1103 14-15 -0.733127705292226 1103 16-17 -0.7794307523374684 1103 18-19 -0.6912287788225484 1103 20-21 -0.7537343576108739 1103 22-23 -0.8031793357279184 1103 24-25 -0.794518539081885 1103 26-27 -0.855066866331768 1103 28-29 -0.9386014866353918 1103 30-31 -0.9209489582108787 1103 32-33 -0.9493887746506076 1103 34-35 -1.0034373693966359 1103 36-37 -0.9565294557883846 1103 38-39 -0.941613090548131 1103 40-41 -1.0387973340169694 1103 42-43 -1.1168265933778017 1103 44-45 -1.2105576947212668 1103 46-47 -1.1474338042800483 1103 48-49 -1.2199672149986398 1103 50-51 -1.2186585562367043 1103 52-53 -1.156260934004635 1103 54-55 -0.8726396549608708 1103 56-57 -0.8147740389249734 1103 58-59 -0.781294250506356 1103 60-61 -0.7777714215763822 1103 62-63 -0.7258115601419419 1103 64-65 -0.7340781798130109 1103 66-67 -0.800913567281313 1103 68-69 -0.7965047946605068 1103 70-71 -0.8147310138548747 1103 72-73 -0.8437436836943562 1103 74-75 -0.9124573705180516 1103 76-77 -0.8533417366419727 1103 78-79 -0.9356035544360495 1103 80-81 -0.8934885160729422 1103 82-83 -0.9292665298322902 1103 84-85 -1.0199855142938041 1103 86-87 -1.0374375016985056 1103 88-89 -1.0429013826824622 1103 90-91 -1.1973051487970672 1103 92-93 -1.2752301840892315 1103 94-95 -1.245939168794731 1103 96-97 -1.226589453798585 1103 98-99 -1.2969438920862082 1103 100 -1.486570161970537 1104 1 -0.5285993738565118 1104 2 -0.4755755069823344 1104 3 -0.5118190879994167 1104 4 -0.3507413340837502 1104 5 -0.3317168939040016 1104 6 -0.3591670897258652 1104 7 -0.4144090863833654 1104 8 -0.3890886397826492 1104 9 -0.48006045791292706 1104 10-11 -0.5174667719185564 1104 12-13 -0.5246260990571088 1104 14-15 -0.6996565613496344 1104 16-17 -0.8026112131121792 1104 18-19 -0.7726887464859402 1104 20-21 -0.6699633009938282 1104 22-23 -0.6600527278130102 1104 24-25 -0.7229611183608782 1104 26-27 -0.7575076512560486 1104 28-29 -0.8298311002721874 1104 30-31 -0.8848496693242822 1104 32-33 -0.9363806842793494 1104 34-35 -1.0489799887649554 1104 36-37 -0.9687627641902523 1104 38-39 -0.9965360976371898 1104 40-41 -0.9639943942021318 1104 42-43 -0.9788024317264998 1104 44-45 -1.008505544084052 1104 46-47 -1.0730759815252355 1104 48-49 -0.9695211444006873 1104 50-51 -1.0551447704482015 1104 52-53 -0.8716907126201647 1104 54-55 -0.7528818361905536 1104 56-57 -0.8353172081977007 1104 58-59 -0.7555349973187404 1104 60-61 -0.852350215174269 1104 62-63 -0.8119081661616221 1104 64-65 -0.7699829006737602 1104 66-67 -0.9008805211745923 1104 68-69 -0.9581482296870263 1104 70-71 -0.8662390313128796 1104 72-73 -0.7193028920305053 1104 74-75 -0.8386620518227161 1104 76-77 -0.8348376850870842 1104 78-79 -0.8794797375914669 1104 80-81 -0.9837002122321366 1104 82-83 -0.944137759256332 1104 84-85 -0.8901288401170824 1104 86-87 -0.9390090673677918 1104 88-89 -0.97441540020845 1104 90-91 -1.0293633272752238 1104 92-93 -1.0953643337300285 1104 94-95 -1.1047179703615981 1104 96-97 -1.1077136761282311 1104 98-99 -1.1233703089158134 1104 100 -1.0405579057309353 1105 1 -0.6126297206834046 1105 2 -0.5883654570403749 1105 3 -0.6256422988141495 1105 4 -0.4783253566642216 1105 5 -0.40868494005575684 1105 6 -0.40208982810395355 1105 7 -0.4105133549260458 1105 8 -0.5215722653402253 1105 9 -0.6965472014860197 1105 10-11 -0.6659501923102127 1105 12-13 -0.718884168516503 1105 14-15 -0.9280724948901664 1105 16-17 -0.8647677264058942 1105 18-19 -0.8392945071563105 1105 20-21 -0.8938192094764119 1105 22-23 -0.8914320574501033 1105 24-25 -0.9332205377882374 1105 26-27 -0.8382426134931151 1105 28-29 -0.9497865020120599 1105 30-31 -0.9293801740298591 1105 32-33 -1.0255439134793036 1105 34-35 -1.0461041830240134 1105 36-37 -1.008772867159486 1105 38-39 -1.0482284688356884 1105 40-41 -1.1003521283374127 1105 42-43 -1.1222704056343673 1105 44-45 -1.1822998022168392 1105 46-47 -1.1787286026819928 1105 48-49 -1.1510157830253789 1105 50-51 -1.1532162628772369 1105 52-53 -1.0196464677013637 1105 54-55 -0.8969688278847912 1105 56-57 -0.8735537414924792 1105 58-59 -0.8642651260134286 1105 60-61 -0.8684907449694634 1105 62-63 -0.9195563779643763 1105 64-65 -0.8744270710504267 1105 66-67 -0.8415859850360192 1105 68-69 -0.9109926682842442 1105 70-71 -0.8258941089479563 1105 72-73 -0.7907597112888922 1105 74-75 -0.8689829070472754 1105 76-77 -0.8552961232864185 1105 78-79 -0.9532488174546856 1105 80-81 -0.9464745252792106 1105 82-83 -0.9913359797510743 1105 84-85 -0.9030819321013333 1105 86-87 -1.0156729032562062 1105 88-89 -1.0445946909429864 1105 90-91 -1.0678799522897542 1105 92-93 -1.0839583206027648 1105 94-95 -1.1876094376976596 1105 96-97 -1.1656028461497314 1105 98-99 -1.1553366168562391 1105 100 -1.1784194450274192 1106 1 -0.5118803505904737 1106 2 -0.5016566474743414 1106 3 -0.586375222393972 1106 4 -0.47176990791584217 1106 5 -0.4190305091415567 1106 6 -0.4170543255604855 1106 7 -0.43472930411112287 1106 8 -0.4452897124587878 1106 9 -0.5568368492580902 1106 10-11 -0.5267002239658183 1106 12-13 -0.6024985735559412 1106 14-15 -0.7788224501977723 1106 16-17 -0.7666709813807202 1106 18-19 -0.7792375263339295 1106 20-21 -0.7755926101765382 1106 22-23 -0.779296282459498 1106 24-25 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-0.8826208907699211 1106 90-91 -0.9977957051452044 1106 92-93 -1.0271870140708437 1106 94-95 -1.01346338835409 1106 96-97 -1.059298096165687 1106 98-99 -0.9923019125095749 1106 100 -1.1670138630949864 1107 1 -0.6164879936504519 1107 2 -0.5556907508773392 1107 3 -0.5413029130097549 1107 4 -0.4563354472879766 1107 5 -0.37589837766600454 1107 6 -0.4091260169918627 1107 7 -0.4282448848539673 1107 8 -0.4429381570966555 1107 9 -0.7045411919936839 1107 10-11 -0.6464213464925237 1107 12-13 -0.6634924265037441 1107 14-15 -0.8775337323504786 1107 16-17 -0.7656929732974049 1107 18-19 -0.8708088574666561 1107 20-21 -0.8616896760618857 1107 22-23 -0.8601550922653445 1107 24-25 -0.897764092054878 1107 26-27 -0.9198284590688459 1107 28-29 -1.0321292942551068 1107 30-31 -0.9787874859135002 1107 32-33 -1.0018026210717537 1107 34-35 -1.0372017920300678 1107 36-37 -1.1346470511083595 1107 38-39 -0.9834531344547628 1107 40-41 -0.9990862271242733 1107 42-43 -0.9950115782382838 1107 44-45 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40-41 1.3146497268363575 2310 42-43 1.3399665374581815 2310 44-45 1.3378259456500885 2310 46-47 1.354481244400823 2310 48-49 1.4287053574277238 2310 50-51 1.3605097638642007 2310 52-53 1.2965709451989724 2310 54-55 1.1953305029061525 2310 56-57 1.1524845274508593 2310 58-59 1.1530964428651274 2310 60-61 1.1525629638071564 2310 62-63 1.076000727725173 2310 64-65 1.106435981219093 2310 66-67 1.0690342863280264 2310 68-69 1.0493065383297449 2310 70-71 1.0502306125889689 2310 72-73 1.0413362909612474 2310 74-75 1.059768979918779 2310 76-77 1.076176675228247 2310 78-79 1.1367501277892629 2310 80-81 1.1024262767842217 2310 82-83 1.157240301553113 2310 84-85 1.1845620495803182 2310 86-87 1.160345235555674 2310 88-89 1.1867643360482987 2310 90-91 1.2808874418344338 2310 92-93 1.3083296771710273 2310 94-95 1.3456786633075737 2310 96-97 1.385051506551715 2310 98-99 1.4589757495226259 2310 100 1.516458195789994 2311 1 0.7113256049127799 2311 2 0.6960722523914882 2311 3 0.7075458760218467 2311 4 0.5785181983534144 2311 5 0.5702167391333504 2311 6 0.6161817460548278 2311 7 0.6341410049359624 2311 8 0.5761777047311298 2311 9 0.7787353084343636 2311 10-11 0.7849045038509033 2311 12-13 0.8939738631781964 2311 14-15 1.070559436611127 2311 16-17 1.0646113832910942 2311 18-19 1.017850036659766 2311 20-21 1.0495386360566386 2311 22-23 1.0854818381170475 2311 24-25 1.1302004544369098 2311 26-27 1.1215765351275948 2311 28-29 1.1516166845921987 2311 30-31 1.132942793925075 2311 32-33 1.1721236567212046 2311 34-35 1.1341769045993715 2311 36-37 1.1893779545327945 2311 38-39 1.2517386371139025 2311 40-41 1.262355985564966 2311 42-43 1.2922813950815026 2311 44-45 1.289989431489083 2311 46-47 1.344704289611137 2311 48-49 1.3342849970962902 2311 50-51 1.3016429681598112 2311 52-53 1.1744186822878149 2311 54-55 1.1131787670054791 2311 56-57 1.046039800070993 2311 58-59 1.0546364872777403 2311 60-61 0.9963775854735175 2311 62-63 0.9688744440354284 2311 64-65 0.9714391792145349 2311 66-67 0.9801800191001746 2311 68-69 1.0583012298968555 2311 70-71 1.0443350181570779 2311 72-73 1.0398326342271318 2311 74-75 1.0452398158771885 2311 76-77 1.069663843720683 2311 78-79 1.0844793953165492 2311 80-81 1.0521068844752222 2311 82-83 1.103567062426901 2311 84-85 1.1606829649496255 2311 86-87 1.15681863709694 2311 88-89 1.1777697762338022 2311 90-91 1.205826416415757 2311 92-93 1.247160384634693 2311 94-95 1.269335325558533 2311 96-97 1.3483743795254206 2311 98-99 1.3874433587395565 2311 100 1.4190921941945902 2312 1 0.5809092360112871 2312 2 0.57022639848757 2312 3 0.6025729921062108 2312 4 0.33840450414249545 2312 5 0.407540423871005 2312 6 0.47882840560023254 2312 7 0.3526568560837475 2312 8 0.48140952197849174 2312 9 0.6669666247208426 2312 10-11 0.5823966566874716 2312 12-13 0.727942983203981 2312 14-15 0.9100972046562603 2312 16-17 0.9407756443351971 2312 18-19 0.9617291220983617 2312 20-21 0.9424010283528759 2312 22-23 1.0026368326085517 2312 24-25 1.016938136371742 2312 26-27 1.0646641503805938 2312 28-29 1.0274491014112357 2312 30-31 1.041925981708033 2312 32-33 1.0666604604025238 2312 34-35 1.0715804211527242 2312 36-37 1.0951701641110319 2312 38-39 1.1083251831420569 2312 40-41 1.1053063495852413 2312 42-43 1.1967858565702016 2312 44-45 1.175163434443256 2312 46-47 1.1982027887993567 2312 48-49 1.1987929046913166 2312 50-51 1.2597695406046654 2312 52-53 1.0983775835399712 2312 54-55 0.9691440773000153 2312 56-57 0.9596458863032851 2312 58-59 0.9213358987004767 2312 60-61 0.8969370623882753 2312 62-63 0.907288648224899 2312 64-65 0.9334839561937542 2312 66-67 0.917307216475372 2312 68-69 0.9597276105796837 2312 70-71 0.9006871447087121 2312 72-73 0.8906715554424167 2312 74-75 0.9623398265703784 2312 76-77 0.9227621248657343 2312 78-79 0.9310158490218186 2312 80-81 0.9744474406378103 2312 82-83 1.0140843487777254 2312 84-85 1.0616409773303808 2312 86-87 1.0755925532254338 2312 88-89 1.0188108858066158 2312 90-91 1.0789914777547587 2312 92-93 1.1538114733821452 2312 94-95 1.2163915638299088 2312 96-97 1.2166116311911495 2312 98-99 0.8106643263527502 2312 100 1.1441326325250074 2313 1 0.5829326196781679 2313 2 0.6133599419136999 2313 3 0.5890745072305208 2313 4 0.456427796975035 2313 5 0.304825437064423 2313 6 0.45182187464307333 2313 7 0.5247163474447234 2313 8 0.54095805930951 2313 9 0.6707591785069908 2313 10-11 0.7188580349920173 2313 12-13 0.7442159125140435 2313 14-15 0.8868021510052273 2313 16-17 0.939299832022563 2313 18-19 0.9094513351636238 2313 20-21 0.9180378758031864 2313 22-23 0.9661996181837793 2313 24-25 0.9301271829155766 2313 26-27 0.9732397387169343 2313 28-29 1.0490333470521094 2313 30-31 1.0319488316594985 2313 32-33 1.0866058037378465 2313 34-35 1.1139956883935724 2313 36-37 1.1630228226792099 2313 38-39 1.2030358760938356 2313 40-41 1.184500157042713 2313 42-43 1.233360068671324 2313 44-45 1.2021159211619192 2313 46-47 1.2252866249684402 2313 48-49 1.1779674503391178 2313 50-51 1.1466738057608126 2313 52-53 1.0589267643927798 2313 54-55 0.9530165355809928 2313 56-57 0.8781498237848169 2313 58-59 0.899811212488494 2313 60-61 0.8508426859644516 2313 62-63 0.839931038004849 2313 64-65 0.8997212851956675 2313 66-67 0.8667669372034865 2313 68-69 0.9209250186264555 2313 70-71 0.9142825835658641 2313 72-73 0.9462093722844642 2313 74-75 0.9340683879986429 2313 76-77 0.7419479643046643 2313 78-79 0.9031191455127114 2313 80-81 0.9854871657183821 2313 82-83 0.9907659358226617 2313 84-85 1.0371023219942934 2313 86-87 1.0714262361144478 2313 88-89 1.1587931477743538 2313 90-91 1.1293213381974745 2313 92-93 1.1924804369284665 2313 94-95 1.2283972801468863 2313 96-97 1.2828332220897245 2313 98-99 1.2747255179772203 2313 100 1.4805958253185239 2314 1 0.5409603690862497 2314 2 0.5660246515102472 2314 3 0.5888365138217964 2314 4 0.44382345238285836 2314 5 0.43057963091693807 2314 6 0.47110550204869384 2314 7 0.5457438008372435 2314 8 0.548023708528099 2314 9 0.671279989470186 2314 10-11 0.702977571704956 2314 12-13 0.7351168590529085 2314 14-15 0.8992907217262598 2314 16-17 0.8999693790806234 2314 18-19 0.872809907202722 2314 20-21 0.8750562650720681 2314 22-23 0.9155735948192358 2314 24-25 0.9182530470203076 2314 26-27 0.9739000180664235 2314 28-29 0.9931872845544518 2314 30-31 0.9905857690316537 2314 32-33 0.9955242631189734 2314 34-35 0.9978651421701272 2314 36-37 1.0624206117587462 2314 38-39 1.0521210985822051 2314 40-41 1.1154248074641444 2314 42-43 1.2154924777927363 2314 44-45 1.2046330208152014 2314 46-47 1.220273184499355 2314 48-49 1.1884483994833417 2314 50-51 1.1947423687830678 2314 52-53 1.1433529704139005 2314 54-55 1.0401851468492822 2314 56-57 0.9753216772344757 2314 58-59 0.9556677073753477 2314 60-61 0.9395392853953268 2314 62-63 0.9189919348491884 2314 64-65 0.9279656016916462 2314 66-67 0.966555536071894 2314 68-69 0.9654244918019472 2314 70-71 0.9924484946073093 2314 72-73 0.9648867669191077 2314 74-75 0.9908264676903045 2314 76-77 1.030577457991484 2314 78-79 1.0536862808003349 2314 80-81 1.030129023442413 2314 82-83 1.0184700095669221 2314 84-85 1.0935298466114283 2314 86-87 1.1655292219060556 2314 88-89 1.1736077927946411 2314 90-91 1.256361902510445 2314 92-93 1.3260499232729188 2314 94-95 1.342859494415194 2314 96-97 1.358172061602012 2314 98-99 1.4318184388885662 2314 100 1.489068369481 2315 1 0.4004185719632041 2315 2 0.4643670266527593 2315 3 0.5211420664012039 2315 4 0.20296430380968644 2315 5 0.295637691743579 2315 6 0.39002678860487094 2315 7 0.3828447449762393 2315 8 0.444155864676425 2315 9 0.5627881873845126 2315 10-11 0.45205818705284884 2315 12-13 0.4857532675710914 2315 14-15 0.6972288640315583 2315 16-17 0.7151617315924241 2315 18-19 0.6016224280371816 2315 20-21 0.7444801558202911 2315 22-23 0.5682786835038485 2315 24-25 0.6882737132069892 2315 26-27 0.7798744667725117 2315 28-29 0.820970928054038 2315 30-31 0.8494299827382008 2315 32-33 0.8895014435431889 2315 34-35 0.9158254798655037 2315 36-37 0.9793996879569065 2315 38-39 0.970012742785741 2315 40-41 0.9468276645952542 2315 42-43 1.0347526777610554 2315 44-45 1.0862224138543795 2315 46-47 1.0656395964169505 2315 48-49 1.0912024473635071 2315 50-51 0.9391254754576508 2315 52-53 1.0115722632955055 2315 54-55 0.8878609055348505 2315 56-57 0.8023853275061654 2315 58-59 0.8303971627801658 2315 60-61 0.8382353189878984 2315 62-63 0.8299545457235737 2315 64-65 0.800385294256472 2315 66-67 0.8128803865490539 2315 68-69 0.8197013580346422 2315 70-71 0.7939090333570107 2315 72-73 0.5696020801466446 2315 74-75 0.7729165651366543 2315 76-77 0.8129250013921094 2315 78-79 0.8577990485025353 2315 80-81 0.5918683733895662 2315 82-83 0.585773110733161 2315 84-85 0.8992483250320298 2315 86-87 1.007168857251429 2315 88-89 0.9675111431791379 2315 90-91 1.048307215156978 2315 92-93 1.119728616952763 2315 94-95 1.1207603706467921 2315 96-97 0.9583686106995017 2315 98-99 0.3842638414870976 2315 100 0.7945341673589468 2316 1 0.31452986111187897 2316 2 0.2873339950088116 2316 3 0.34184768012964994 2316 4 0.21493197477035153 2316 5 0.1764476229518408 2316 6 0.2319042564386251 2316 7 0.20787937129884426 2316 8 0.21544992208638547 2316 9 0.3117358201834364 2316 10-11 0.2544405865564485 2316 12-13 0.06160720641438644 2316 14-15 0.13331729156561067 2316 16-17 0.3667099208133493 2316 18-19 0.3876109849121221 2316 20-21 0.40438733718657005 2316 22-23 0.3721255742186429 2316 24-25 0.4336032973000883 2316 26-27 0.5062697865236814 2316 28-29 0.47846092962220155 2316 30-31 0.3810077427400955 2316 32-33 0.5927184340599965 2316 34-35 0.526737050226231 2316 36-37 0.5600331472015654 2316 38-39 0.7068234978948951 2316 40-41 0.7510974601532538 2316 42-43 0.6936514914987697 2316 44-45 0.6053956314005333 2316 46-47 0.5020697855983727 2316 48-49 0.40219310553507626 2316 50-51 0.5094406920120349 2316 52-53 0.636270218002096 2316 54-55 0.24690546012289616 2316 56-57 0.16516165725123955 2316 58-59 0.18866151371231155 2316 60-61 0.30108497407513823 2316 62-63 0.40997487763012685 2316 64-65 0.3990792636549614 2316 66-67 0.33698340387888237 2316 68-69 0.31844037289481975 2316 70-71 0.08327348591733852 2316 72-73 0.03297737853301186 2316 74-75 0.24294121038425232 2316 76-77 0.39177277282622214 2316 78-79 0.43245458198865805 2316 80-81 0.6534517002318125 2316 82-83 0.7129126623830864 2316 84-85 0.7121094311501004 2316 86-87 0.7515496020553201 2316 88-89 0.7383312106660895 2316 90-91 0.5464280160070949 2316 92-93 0.5944744552432475 2316 94-95 0.781278422962842 2316 96-97 0.6775811941035634 2316 98-99 0.5706687932729544 2316 100 0.4509783833252534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 25.0 1 720.0 2 249.0 3 223.0 4 199.0 5 159.0 6 185.0 7 716.0 8 2469.0 9 5070.0 10 7717.0 11 11127.0 12 15174.0 13 18698.0 14 22350.0 15 26805.0 16 32817.0 17 41511.0 18 52665.0 19 64842.0 20 83857.0 21 106391.0 22 117789.0 23 114640.0 24 109936.0 25 112089.0 26 123369.0 27 144257.0 28 171391.0 29 208801.0 30 256512.0 31 323216.0 32 416316.0 33 558026.0 34 823566.0 35 1142815.0 36 1731564.0 37 2257352.0 38 1661557.0 39 512037.0 40 293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.048093784880834 21.334673029484165 12.866786839552166 22.750446346082832 2 15.597282010255666 19.496314003378313 41.71409761881174 23.192306367554284 3 19.27693472291152 26.987256387035824 28.00969286737366 25.726116022678998 4 12.428913534118267 16.09592220499344 39.81337760501112 31.661786655877172 5 13.0045744101359 38.735740049857675 34.198324581386984 14.061360958619442 6 29.48404306181474 38.35474283670583 17.800546300250122 14.36066780122931 7 27.40114831135249 31.882516527211962 23.165312037753676 17.55102312368187 8 26.763747087090668 34.99600672417268 19.35652200754447 18.883724181192186 9 26.659654027359935 15.171549497644321 22.002353905318724 36.16644256967702 10-11 26.601214814311575 25.964450821155534 26.19394856247505 21.240385802057844 12-13 26.40116243799071 23.65185344043117 27.30992687844282 22.6370572431353 14-15 23.763510105908367 24.640508980874422 25.34544749195019 26.25053342126702 16-17 21.75143363293579 27.466262524711137 26.695643395414905 24.08666044693817 18-19 21.07513230065476 27.67362773400885 29.408767857296446 21.84247210803994 20-21 22.95066499981467 26.733767568496496 28.576614153971224 21.738953277717606 22-23 23.52952235282099 26.402771116988372 28.222453950691555 21.845252579499082 24-25 22.75305432369114 26.238159344043243 29.31237357249037 21.69641275977525 26-27 22.617774468286036 26.604321812947262 28.048147932211776 22.729755786554925 28-29 21.549860793097167 27.16358154470626 28.82312663013243 22.46343103206414 30-31 22.739048194647058 26.71378272347408 28.679230593799222 21.867938488079645 32-33 23.09409330838498 26.965224873705278 27.619110452951308 22.321571364958434 34-35 23.044142952597284 26.73196986579184 28.638551337536917 21.58533584407396 36-37 22.453638478944036 26.32191261606314 28.20178383409716 23.022665070895656 38-39 22.888125189072014 26.527388179879463 28.72936912031207 21.855117510736452 40-41 22.470339910226613 25.392792368175964 29.430284628231018 22.706583093366405 42-43 22.429957763809906 25.674566275344485 28.577578236722207 23.317897724123405 44-45 22.6348267312803 26.68723966032786 28.38607485613842 22.291858752253418 46-47 22.592422439630695 26.57461376645082 27.6068804890684 23.226083304850082 48-49 23.490064465134154 26.44807084198022 27.57130271154095 22.490561981344673 50-51 22.97134987882243 26.152632389674498 27.704223155082726 23.17179457642035 52-53 22.432735385739853 26.882808092795763 27.316425160512726 23.36803136095165 54-55 22.264795122456743 26.749754698944756 27.4125586601771 23.572891518421404 56-57 23.4584772647522 26.229822901883566 26.808602479931494 23.503097353432743 58-59 22.33826803996979 26.790009867698515 27.775502519343036 23.096219572988662 60-61 22.398056598995925 27.608467782215403 26.985492463087148 23.00798315570153 62-63 22.897555991422266 26.644244499114013 26.545429586371398 23.912769923092327 64-65 22.01936850633321 26.838487489501034 27.08761160226852 24.054532401897237 66-67 22.6880757678242 26.352814107158075 26.98595972781292 23.973150397204805 68-69 22.084755965120173 26.53971722468777 27.313709920674373 24.061816889517683 70-71 22.118306677234028 26.115324673205283 27.48237955087766 24.28398909868303 72-73 22.971942309745945 26.26470168758752 27.650973572706544 23.11238242995999 74-75 23.20499035388024 26.640498804821156 27.111546921910108 23.0429639193885 76-77 22.87201478375915 26.516785135370004 26.614428707733452 23.9967713731374 78-79 22.736433935303985 26.655029123121203 26.67377475483037 23.934762186744443 80-81 22.23268865664292 27.155038231404426 27.41567092300517 23.196602188947484 82-83 23.351695660717464 26.040049104143776 28.657853470737916 21.950401764400844 84-85 23.492349254875684 26.55283350643454 27.105573645950248 22.84924359273953 86-87 22.035849552063482 27.436824016992905 27.51054353603556 23.016782894908054 88-89 23.29939969149945 25.94237270716704 27.356357950198234 23.401869651135275 90-91 22.911162271537723 26.11300049990412 27.469755800716715 23.506081427841444 92-93 23.304942680000707 26.435252293921696 27.351109569371328 22.908695456706273 94-95 22.013985596026135 26.502582095344035 28.0989932683556 23.384439040274234 96-97 22.466033452702757 26.369575933932072 27.53979269039849 23.624597922966682 98-99 21.830088520845358 26.990657066602957 27.275194214528085 23.904060198023593 100 22.817849954559417 26.344390305157805 26.240401962919634 24.59735777736314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1603.0 1 1120.5 2 1015.0 3 1385.0 4 1130.5 5 988.0 6 1524.5 7 2899.5 8 4515.5 9 5199.0 10 4856.0 11 4631.0 12 5254.0 13 6444.0 14 7925.5 15 9639.5 16 11286.5 17 12643.0 18 13880.5 19 14954.0 20 15892.0 21 16714.0 22 17844.0 23 19440.0 24 20998.5 25 22740.0 26 26167.5 27 30165.0 28 36624.0 29 53340.0 30 80163.0 31 107346.5 32 146942.0 33 192749.5 34 226388.5 35 260606.0 36 292520.0 37 347825.5 38 369545.5 39 341777.5 40 312937.0 41 279831.5 42 269343.0 43 285502.0 44 316947.0 45 384460.0 46 452126.0 47 499162.0 48 537336.5 49 532257.5 50 507364.0 51 531732.0 52 542068.5 53 511814.0 54 481126.0 55 408359.0 56 352743.0 57 289384.5 58 223033.5 59 214640.0 60 179139.5 61 120997.0 62 88784.5 63 76935.5 64 57638.0 65 27054.5 66 14352.5 67 9187.5 68 6804.5 69 5581.5 70 4612.5 71 3699.0 72 3000.0 73 2760.0 74 2276.5 75 1688.5 76 1307.0 77 926.5 78 641.5 79 495.5 80 374.5 81 271.5 82 197.5 83 144.5 84 116.0 85 94.0 86 77.5 87 64.5 88 48.0 89 33.5 90 29.5 91 28.5 92 23.5 93 19.0 94 13.5 95 10.5 96 6.5 97 3.0 98 4.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05578263920503533 2 0.13031611787584463 3 0.017057501244514935 4 0.016454637375166177 5 0.019451225431635015 6 0.05178423324803105 7 0.05691744178263299 8 0.04914227099706148 9 0.04091495230947839 10-11 0.03935016594271286 12-13 0.040524863923429195 14-15 0.018241064870368754 16-17 0.017194918744145905 18-19 0.017394395759739244 20-21 0.02082540042794469 22-23 0.02559511751190989 24-25 0.02461103090164941 26-27 0.01921628583549175 28-29 0.01805931914505038 30-31 0.016055683343979495 32-33 0.023037378889746392 34-35 0.023139333808827434 36-37 0.023099438405708765 38-39 0.02106920566922544 40-41 0.024535672917980814 42-43 0.03209363539768403 44-45 0.04150451771112093 46-47 0.0472139931796592 48-49 0.0397668512641745 50-51 0.027199799281794088 52-53 0.03949201626491257 54-55 0.03603441466129468 56-57 0.038162169494290306 58-59 0.04281663319146824 60-61 0.022727081309934533 62-63 0.02532914815778543 64-65 0.024704120175592967 66-67 0.03168581572135987 68-69 0.038335049574471196 70-71 0.04013477553738 72-73 0.04378742133402249 74-75 0.02606499670419642 76-77 0.019992029785021403 78-79 0.019513284947597388 80-81 0.023986002919456944 82-83 0.019850179462821693 84-85 0.012504992466418044 86-87 0.014304718429326846 88-89 0.018116945838444008 90-91 0.020679117283176243 92-93 0.019433494141360055 94-95 0.02311273687341499 96-97 0.024274136386425102 98-99 0.027585454845274544 100 0.02864489943920362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1279495E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.532812311474665 #Duplication Level Percentage of deduplicated Percentage of total 1 75.67912288500149 47.32428387264825 2 14.646501979240567 18.31773918574985 3 4.831160064281194 9.063180766391636 4 2.0140038139515424 5.037652899697037 5 1.0339421508296927 3.23276552293778 6 0.5884823196559855 2.207967266620139 7 0.324561641536502 1.4207026549594337 8 0.20774748422399 1.0392827551327846 9 0.13830277064086913 0.7783615078778158 >10 0.49224960047059396 5.032532122259633 >50 0.023858166268988616 1.030592634724881 >100 0.01748314073048186 2.090387382077746 >500 0.001591822836471278 0.7064315360168659 >1k 8.049603120499476E-4 1.0079693608859095 >5k 1.1232001177949828E-4 0.4543856735635508 >10k+ 7.488000785299885E-5 1.2557648584567278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41145 0.36477696918168767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31181 0.27643968103181926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19662 0.17431631469316666 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 15286 0.13552025157154643 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.865645137481775E-6 0.0 5 0.0 0.0 0.0 1.773129027496355E-5 0.0 6 0.0 0.0 0.0 1.773129027496355E-5 0.0 7 0.0 0.0 0.0 1.773129027496355E-5 0.0 8 0.0 0.0 0.0 2.6596935412445328E-5 0.0 9 0.0 0.0 0.0 2.6596935412445328E-5 0.0 10-11 0.0 0.0 0.0 2.6596935412445328E-5 0.0 12-13 0.0 0.0 0.0 2.6596935412445328E-5 0.0 14-15 0.0 0.0 0.0 3.54625805499271E-5 0.0 16-17 0.0 0.0 0.0 4.876104825614977E-5 0.0 18-19 0.0 0.0 0.0 5.3193870824890656E-5 0.0 20-21 0.0 0.0 0.0 8.422362880607687E-5 0.0 22-23 0.0 0.0 0.0 1.9947701559333995E-4 0.0 24-25 0.0 0.0 0.0 5.319387082489066E-4 0.0 26-27 0.0 0.0 0.0 0.0012544887869536713 0.0 28-29 0.0 0.0 0.0 0.0037944961188421996 0.0 30-31 0.0 0.0 0.0 0.014539658025470113 0.0 32-33 0.0 0.0 0.0 0.041114429325071734 0.0 34-35 0.0 0.0 0.0 0.07882445091735046 0.0 36-37 0.0 0.0 0.0 0.14439476235416568 0.0 38-39 0.0 0.0 0.0 0.24413770297340442 0.0 40-41 0.0 0.0 0.0 0.3516469487330771 0.0 42-43 0.0 0.0 0.0 0.4556321005506009 0.0 44-45 0.0 0.0 0.0 0.5750523405524803 0.0 46-47 0.0 0.0 0.0 0.7186137322637228 0.0 48-49 0.0 0.0 0.0 0.8798354890888289 0.0 50-51 0.0 0.0 0.0 1.0483182092815326 0.0 52-53 0.0 0.0 0.0 1.207789001191986 0.0 54-55 0.0 0.0 0.0 1.377189315656419 0.0 56-57 0.0 0.0 0.0 1.5768170472170961 0.0 58-59 0.0 0.0 0.0 1.8109498696528523 0.0 60-61 0.0 8.865645137481775E-6 0.0 2.0474498193403163 0.0 62-63 0.0 8.865645137481775E-6 0.0 2.2907674501385036 0.0 64-65 0.0 8.865645137481775E-6 0.0 2.535162256820895 0.0 66-67 0.0 8.865645137481775E-6 0.0 2.8065174903663683 0.0 68-69 0.0 8.865645137481775E-6 0.0 3.105440447466841 0.0 70-71 0.0 8.865645137481775E-6 0.0 3.429240404823088 0.0 72-73 0.0 8.865645137481775E-6 0.0 3.7585769575676924 0.0 74-75 0.0 8.865645137481775E-6 0.0 4.070714158745583 0.0 76-77 0.0 8.865645137481775E-6 0.0 4.425570471018428 0.0 78-79 0.0 1.773129027496355E-5 0.0 4.801620107992424 0.0 80-81 0.0 1.773129027496355E-5 0.0 5.205312826505088 0.0 82-83 0.0 1.773129027496355E-5 0.0 5.616067031369756 0.0 84-85 0.0 1.773129027496355E-5 0.0 6.006585401208121 0.0 86-87 0.0 2.6596935412445328E-5 0.0 6.41673674220344 0.0 88 0.0 2.6596935412445328E-5 0.0 6.748369497038653 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 13630 0.0 38.757355 94 GGTATCA 28170 0.0 38.521008 1 GTACTAG 14070 0.0 38.160732 1 GCATCGT 13910 0.0 36.32708 8 GTATCAA 46375 0.0 35.991707 1 TAGGCAT 16970 0.0 34.070927 5 TACCTGG 4855 0.0 32.663734 2 ACCTGGG 4765 0.0 32.258602 3 TACCCCG 645 0.0 32.066654 5 CAACGCA 50310 0.0 31.216402 5 CTAGGCA 19250 0.0 30.865763 4 TATTCTC 5545 0.0 30.603165 5 ATCAACG 51395 0.0 30.593975 3 CTGTGCG 19160 0.0 30.396189 9 TAGTACT 19755 0.0 30.005354 4 ACTGTGC 20035 0.0 29.772919 8 TATCAAC 56825 0.0 29.24869 2 CATCGTC 18005 0.0 28.691149 9 GTACTGT 20890 0.0 28.35272 6 AAGCTAA 7085 0.0 28.262663 9 >>END_MODULE