FastQCFastQC Report
Mon 6 Jun 2016
ERR1211197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11279495
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363660.32240805106966225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299060.26513598348152995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189320.167844393742805No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC156270.1385434365634277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC143350.042.10736594
GGTATCA282250.036.6124531
GTACTAG137300.036.0230331
GTATCAA455200.035.0958821
TATTCTC54350.033.6294175
TAGGCAT165350.033.3037155
CTGTGCG200850.032.8211029
ACCTGGG45650.032.3274733
TAGTACT207100.031.8617734
ACTGTGC211450.031.7535218
TACCTGG49350.030.3766732
GTACTGT219250.030.2596826
CAACGCA502450.030.2237115
TACTGTG232500.029.990267
ATCAACG510800.029.6914223
CTAGGCA190650.029.631294
TACCCCG6850.029.4949175
CTAGTAC241950.028.7293283
TATCAAC572400.027.8725572
CTAACTG51750.027.2454384
Produced by FastQC (version 0.11.5)