##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211197_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11279495 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.104245269845855 33.0 31.0 34.0 30.0 34.0 2 32.25302223193503 33.0 31.0 34.0 30.0 34.0 3 32.329206139104635 34.0 31.0 34.0 30.0 34.0 4 35.95496438448707 37.0 35.0 37.0 35.0 37.0 5 35.841575620185125 37.0 35.0 37.0 35.0 37.0 6 35.86783707958557 37.0 35.0 37.0 35.0 37.0 7 35.80713152494859 37.0 35.0 37.0 35.0 37.0 8 35.79976967053933 37.0 35.0 37.0 35.0 37.0 9 37.3827949744204 39.0 37.0 39.0 34.0 39.0 10-11 37.35921847564984 39.0 37.0 39.0 34.0 39.0 12-13 37.236022623353264 39.0 37.0 39.0 33.5 39.0 14-15 38.412769631973774 40.0 38.0 41.0 33.5 41.0 16-17 38.37700592978675 40.0 38.0 41.0 34.0 41.0 18-19 38.40812594003543 40.0 38.0 41.0 34.0 41.0 20-21 38.36933683644524 40.0 38.0 41.0 34.0 41.0 22-23 38.221094206788514 40.0 38.0 41.0 34.0 41.0 24-25 38.14287949061549 40.0 37.0 41.0 33.0 41.0 26-27 37.983721567321936 40.0 37.0 41.0 33.0 41.0 28-29 37.97150413205556 40.0 37.0 41.0 33.0 41.0 30-31 37.98016675391939 40.0 37.0 41.0 33.0 41.0 32-33 37.84442685598957 40.0 37.0 41.0 33.0 41.0 34-35 37.69578314454681 40.0 36.5 41.0 33.0 41.0 36-37 37.5574007524273 40.0 36.0 41.0 32.5 41.0 38-39 37.44818393908592 40.0 36.0 41.0 32.0 41.0 40-41 37.330448570614195 39.0 36.0 41.0 32.0 41.0 42-43 37.163512018933474 39.0 36.0 41.0 31.0 41.0 44-45 36.95953032471755 39.0 35.0 41.0 31.0 41.0 46-47 36.666668410243545 39.0 35.0 41.0 30.5 41.0 48-49 36.35705743031936 39.0 35.0 40.0 30.0 41.0 50-51 36.10766749752538 38.0 35.0 40.0 29.5 41.0 52-53 35.859034070230976 38.0 35.0 40.0 29.0 41.0 54-55 35.58379825515238 38.0 34.0 40.0 28.5 41.0 56-57 35.27010721667947 37.0 34.0 40.0 28.0 41.0 58-59 34.93688338883966 36.5 34.0 40.0 27.5 41.0 60-61 34.655897360653114 36.0 34.0 39.0 27.0 41.0 62-63 34.32293644351986 35.5 33.0 39.0 26.5 40.5 64-65 33.93832144080919 35.0 33.0 38.5 26.0 40.0 66-67 33.643698720554426 35.0 33.0 38.0 26.0 40.0 68-69 33.34585661858088 35.0 32.5 37.5 25.5 40.0 70-71 33.093407062993506 35.0 32.0 37.0 25.0 40.0 72-73 32.810233392541065 35.0 32.0 36.5 25.0 39.0 74-75 32.51413241461608 35.0 32.0 36.0 24.5 39.0 76-77 31.585235065931585 34.0 30.5 35.0 23.5 37.5 78-79 32.12668528156624 34.5 31.5 35.0 24.0 37.5 80-81 32.10436353755199 35.0 31.5 35.0 24.5 37.0 82-83 31.89648938183846 34.0 31.0 35.0 24.0 37.0 84-85 31.682477805965604 34.0 31.0 35.0 24.0 36.0 86-87 31.481107221555575 34.0 31.0 35.0 24.0 36.0 88-89 31.293931288590493 34.0 31.0 35.0 23.0 36.0 90-91 31.09245241032511 34.0 31.0 35.0 23.0 35.0 92-93 30.903353474601477 34.0 30.5 35.0 20.5 35.0 94-95 30.72827830501277 34.0 30.0 35.0 20.0 35.0 96-97 30.568697002835677 34.0 30.0 35.0 20.0 35.0 98-99 30.405400197437917 34.0 29.0 35.0 20.0 35.0 100 28.525583015906296 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3830596755056703 1101 2 -0.3477610959006263 1101 3 -0.430447863226167 1101 4 -0.24689681170705313 1101 5 -0.3224246718381991 1101 6 -0.3432688560746584 1101 7 -0.33406595089552127 1101 8 -0.3662589190883736 1101 9 -0.5255560184278423 1101 10-11 -0.4819633031038393 1101 12-13 -0.4650081810859348 1101 14-15 -0.6934147160947006 1101 16-17 -0.6463169397695694 1101 18-19 -0.7539049177774757 1101 20-21 -0.6928177788613965 1101 22-23 -0.7440565933152286 1101 24-25 -0.7308610458951534 1101 26-27 -0.8201377827198755 1101 28-29 -0.8414944553091672 1101 30-31 -0.7060939484847992 1101 32-33 -0.7066785052465647 1101 34-35 -0.7105193950559396 1101 36-37 -0.6999753928648431 1101 38-39 -0.7206807650786686 1101 40-41 -0.7597808303675677 1101 42-43 -0.7776890886197947 1101 44-45 -0.8100533334076161 1101 46-47 -0.8167782902444927 1101 48-49 -0.7641439232574783 1101 50-51 -0.8934226654203385 1101 52-53 -0.8809553909743002 1101 54-55 -0.7648040252915251 1101 56-57 -0.7804826961874269 1101 58-59 -0.7692996630696172 1101 60-61 -0.8433908362649731 1101 62-63 -0.9784445080536486 1101 64-65 -0.8818838296572977 1101 66-67 -1.0487718140750104 1101 68-69 -1.0949496809926487 1101 70-71 -1.068448821484651 1101 72-73 -1.1176186332523912 1101 74-75 -1.1507250933985347 1101 76-77 -1.0702496619099584 1101 78-79 -0.9245654842757034 1101 80-81 -0.8701174607018345 1101 82-83 -0.8253140715210314 1101 84-85 -0.7942285855011626 1101 86-87 -0.8824411012639821 1101 88-89 -0.9689017669874183 1101 90-91 -1.0529498091476661 1101 92-93 -1.1566951076808074 1101 94-95 -1.1749741169982926 1101 96-97 -1.118084935016256 1101 98-99 -1.2311202446440923 1101 100 -1.044703811964773 1102 1 -0.45091981794220715 1102 2 -0.394397648266569 1102 3 -0.440256973501139 1102 4 -0.2850241135689089 1102 5 -0.3558068342466072 1102 6 -0.37644220497863046 1102 7 -0.415046608659587 1102 8 -0.43527345227735026 1102 9 -0.5838727481675079 1102 10-11 -0.6204562404465364 1102 12-13 -0.6011874987715444 1102 14-15 -0.726247719598696 1102 16-17 -0.795814197066882 1102 18-19 -0.7800192976313838 1102 20-21 -0.8325304888103631 1102 22-23 -0.7906594122135573 1102 24-25 -0.8357401191433738 1102 26-27 -0.9611858513080449 1102 28-29 -0.8735557735350241 1102 30-31 -0.7808227784030706 1102 32-33 -0.8562588747464659 1102 34-35 -0.8355309500807238 1102 36-37 -0.9251875231755022 1102 38-39 -0.9762062474307527 1102 40-41 -0.926785814609083 1102 42-43 -0.8759272778767269 1102 44-45 -0.9118897910183037 1102 46-47 -0.9692079878610258 1102 48-49 -0.9671288522797354 1102 50-51 -0.9545894866931661 1102 52-53 -0.9805083838805544 1102 54-55 -0.9121051670486935 1102 56-57 -0.960479369218227 1102 58-59 -1.0126496538652034 1102 60-61 -0.9929828192387262 1102 62-63 -1.0174412697648236 1102 64-65 -0.8772648018420952 1102 66-67 -0.9838929753865173 1102 68-69 -1.123090545235307 1102 70-71 -1.1141189314747493 1102 72-73 -1.0788052871322158 1102 74-75 -1.1245004523419553 1102 76-77 -0.9853055220989226 1102 78-79 -0.8141705310238478 1102 80-81 -0.8416137207290575 1102 82-83 -0.8761867840018702 1102 84-85 -0.9890056465485202 1102 86-87 -0.9650360557506623 1102 88-89 -1.071359120798899 1102 90-91 -1.1280302925731647 1102 92-93 -1.105776074902444 1102 94-95 -1.174351512653022 1102 96-97 -1.29950837150081 1102 98-99 -1.3441911922910599 1102 100 -1.384843499820743 1103 1 -0.3817662502210162 1103 2 -0.36664195103160324 1103 3 -0.402857534977624 1103 4 -0.26157039928242654 1103 5 -0.27509938033936265 1103 6 -0.37964951600449837 1103 7 -0.406197831900613 1103 8 -0.3885406657888524 1103 9 -0.6033921460744622 1103 10-11 -0.5210338878714325 1103 12-13 -0.5790043611216404 1103 14-15 -0.7110250034531518 1103 16-17 -0.6646710635673188 1103 18-19 -0.6478112893369428 1103 20-21 -0.6898524132092305 1103 22-23 -0.7755442098981646 1103 24-25 -0.8627971180639378 1103 26-27 -0.843542348545057 1103 28-29 -0.8676178823171696 1103 30-31 -0.8205225004129062 1103 32-33 -0.75195715546473 1103 34-35 -0.9174206986841327 1103 36-37 -0.9316329728834702 1103 38-39 -0.8595952274455954 1103 40-41 -0.894463984907425 1103 42-43 -0.8503164678594288 1103 44-45 -0.854240727672142 1103 46-47 -0.9252726359396348 1103 48-49 -0.9282162729954209 1103 50-51 -1.004285367524112 1103 52-53 -1.037646267960838 1103 54-55 -0.9822347798659621 1103 56-57 -0.9383250306617228 1103 58-59 -0.9986933133279337 1103 60-61 -1.0227666754510736 1103 62-63 -0.9315057313084836 1103 64-65 -0.9814669730003658 1103 66-67 -1.121923293237522 1103 68-69 -1.1019483156299188 1103 70-71 -1.1203558430666547 1103 72-73 -1.0275700706204063 1103 74-75 -1.016929242173596 1103 76-77 -0.9346965466080128 1103 78-79 -0.8441669314156961 1103 80-81 -0.8045501416520011 1103 82-83 -0.807257393362832 1103 84-85 -0.910482657723275 1103 86-87 -0.8859895306017371 1103 88-89 -1.0021202281041752 1103 90-91 -0.9985380425001829 1103 92-93 -1.0894586172039666 1103 94-95 -1.142417941521991 1103 96-97 -1.1101520763587835 1103 98-99 -1.2199351874387006 1103 100 -1.2008090580361142 1104 1 -0.35404329213084296 1104 2 -0.3881927599391233 1104 3 -0.4304019315184604 1104 4 -0.24411398966712028 1104 5 -0.2483935972632807 1104 6 -0.3634255773070265 1104 7 -0.36860023868219116 1104 8 -0.3757074339070172 1104 9 -0.5143138547225945 1104 10-11 -0.5261902136568679 1104 12-13 -0.5690677923162326 1104 14-15 -0.6840733371983916 1104 16-17 -0.6414666532525786 1104 18-19 -0.6957794407257296 1104 20-21 -0.7669981957165248 1104 22-23 -0.7575197601487957 1104 24-25 -0.7652247478678547 1104 26-27 -0.8031104980675039 1104 28-29 -0.9305439517627221 1104 30-31 -0.8555089087017791 1104 32-33 -0.7697386196797993 1104 34-35 -0.8318762003832063 1104 36-37 -0.7054149031656607 1104 38-39 -0.834524567507728 1104 40-41 -0.8503403781003911 1104 42-43 -0.7447067634872866 1104 44-45 -0.8783914549494085 1104 46-47 -0.9332965465234722 1104 48-49 -0.9390691084321006 1104 50-51 -0.7658808516277276 1104 52-53 -0.7604421085654351 1104 54-55 -0.8091266435545492 1104 56-57 -0.7804091883270559 1104 58-59 -0.8915262685911927 1104 60-61 -0.8198845117057658 1104 62-63 -0.8627948434902422 1104 64-65 -0.8696302215822556 1104 66-67 -0.892968088264638 1104 68-69 -0.8867191595432047 1104 70-71 -0.9352435529588661 1104 72-73 -0.9587121620656944 1104 74-75 -1.038049542592482 1104 76-77 -0.8562735139308053 1104 78-79 -0.6963123399139377 1104 80-81 -0.7757786621455125 1104 82-83 -0.7219920224610199 1104 84-85 -0.7602839830039834 1104 86-87 -0.8019422627452677 1104 88-89 -0.8112014289502198 1104 90-91 -0.8766969007342347 1104 92-93 -0.8719769360562388 1104 94-95 -0.9581423670607521 1104 96-97 -0.9999390148813987 1104 98-99 -1.0121189185337478 1104 100 -1.1833859204141888 1105 1 -0.4113068606554222 1105 2 -0.3938711963184822 1105 3 -0.44925596508693033 1105 4 -0.2640125144801502 1105 5 -0.3289507248181138 1105 6 -0.39190192267756885 1105 7 -0.5047863307342695 1105 8 -0.4953432323516793 1105 9 -0.607433912586238 1105 10-11 -0.6098149126546488 1105 12-13 -0.6871818130942344 1105 14-15 -0.9185716321942863 1105 16-17 -0.8060950267244422 1105 18-19 -0.7723044040888283 1105 20-21 -0.8800406268835985 1105 22-23 -0.8832117528258507 1105 24-25 -0.9223223435638062 1105 26-27 -0.9457946751817232 1105 28-29 -0.957185695437694 1105 30-31 -0.9059386136140972 1105 32-33 -0.8936788145568855 1105 34-35 -0.8888263302750659 1105 36-37 -0.8663137489508728 1105 38-39 -0.9222132317885183 1105 40-41 -0.977309610623756 1105 42-43 -0.9389101518316352 1105 44-45 -0.9442177583015336 1105 46-47 -0.9547787284325722 1105 48-49 -1.0270585374700048 1105 50-51 -1.0466798779313962 1105 52-53 -1.0030764822839657 1105 54-55 -0.9439190842107337 1105 56-57 -0.9918489078174062 1105 58-59 -1.0349544422460468 1105 60-61 -0.9600136410844442 1105 62-63 -1.0392588288428541 1105 64-65 -1.0368402383728608 1105 66-67 -1.0582741741870763 1105 68-69 -1.0556935921363078 1105 70-71 -1.0806567200000288 1105 72-73 -1.0776534861376064 1105 74-75 -1.0319015675239385 1105 76-77 -1.0816294188933888 1105 78-79 -0.9450339732939739 1105 80-81 -0.952162013340228 1105 82-83 -0.9232688209100424 1105 84-85 -1.0086247203266687 1105 86-87 -1.0017623384100744 1105 88-89 -0.9862481751575451 1105 90-91 -0.9659686913168919 1105 92-93 -1.0159386230699532 1105 94-95 -1.0525667259628548 1105 96-97 -1.1076831328156018 1105 98-99 -1.1659335055257571 1105 100 -1.1684159035818666 1106 1 -0.4499600198711242 1106 2 -0.4399534487633119 1106 3 -0.4813536395410445 1106 4 -0.28867641398773003 1106 5 -0.3411290782278513 1106 6 -0.38132918225804957 1106 7 -0.42449543546457846 1106 8 -0.38813917847419077 1106 9 -0.5461694988388146 1106 10-11 -0.5833769483633304 1106 12-13 -0.6385976058523255 1106 14-15 -0.7354216769298958 1106 16-17 -0.7538815341445328 1106 18-19 -0.7580532293018791 1106 20-21 -0.7417553714267697 1106 22-23 -0.7035705556862197 1106 24-25 -0.7850183310582892 1106 26-27 -0.7818816446369041 1106 28-29 -0.7853394407323933 1106 30-31 -0.8130498920585865 1106 32-33 -0.8269734168824456 1106 34-35 -0.8257557099532704 1106 36-37 -0.8031283545262653 1106 38-39 -0.7652119827063117 1106 40-41 -0.8303088790513939 1106 42-43 -0.764351012953604 1106 44-45 -0.8370379593215134 1106 46-47 -0.8283745103204367 1106 48-49 -0.8853103146859453 1106 50-51 -0.9173882286170922 1106 52-53 -0.9312824899501706 1106 54-55 -0.8967561113230573 1106 56-57 -0.8879296369034151 1106 58-59 -0.8870872688350886 1106 60-61 -0.9084306317859756 1106 62-63 -0.9494728614768704 1106 64-65 -0.844654388453165 1106 66-67 -0.9164769284198115 1106 68-69 -0.9317289719467539 1106 70-71 -0.9536034357896739 1106 72-73 -1.000080111741532 1106 74-75 -1.0238986487972568 1106 76-77 -0.9782934719613046 1106 78-79 -0.8517364387727895 1106 80-81 -0.7989136199255746 1106 82-83 -0.9204111546266027 1106 84-85 -0.8739822551618879 1106 86-87 -0.9745501531097176 1106 88-89 -0.9416294905962523 1106 90-91 -0.9512779302756336 1106 92-93 -0.895347162263203 1106 94-95 -0.9163396255031415 1106 96-97 -1.0130835316547113 1106 98-99 -1.0172856647410278 1106 100 -1.094333397748084 1107 1 -0.4390943490223229 1107 2 -0.45610301282351884 1107 3 -0.4656509542765441 1107 4 -0.2517681267852794 1107 5 -0.34058673524496896 1107 6 -0.35671466911463057 1107 7 -0.4352496821692142 1107 8 -0.4537994846633566 1107 9 -0.570476165622317 1107 10-11 -0.5741968958477699 1107 12-13 -0.5579099483303267 1107 14-15 -0.7277493013970471 1107 16-17 -0.7246076958451155 1107 18-19 -0.7452406436344603 1107 20-21 -0.7243248730407714 1107 22-23 -0.7683852884024915 1107 24-25 -0.8001929903601521 1107 26-27 -0.7890751171466306 1107 28-29 -0.8216743864734042 1107 30-31 -0.8505964993055386 1107 32-33 -0.7507405606231785 1107 34-35 -0.8231010371073566 1107 36-37 -0.8003648827578687 1107 38-39 -0.8483502151275104 1107 40-41 -0.8257352821610979 1107 42-43 -0.8097014654444621 1107 44-45 -0.8732342203382331 1107 46-47 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2310 14-15 0.935479745646262 2310 16-17 0.7637778564699218 2310 18-19 0.7948144138064208 2310 20-21 0.9108085489853792 2310 22-23 0.9647309641958159 2310 24-25 0.9854566917757595 2310 26-27 1.0317122555380038 2310 28-29 0.998626937996427 2310 30-31 0.8645754116491844 2310 32-33 0.9745677769353449 2310 34-35 1.0336995860536007 2310 36-37 0.9206213842999702 2310 38-39 1.002844884521906 2310 40-41 1.0768540426322986 2310 42-43 1.127113545021004 2310 44-45 1.1324743471860401 2310 46-47 0.7532431700984006 2310 48-49 0.9609730633205444 2310 50-51 1.053570398474534 2310 52-53 1.1033457777066715 2310 54-55 1.1478733502709701 2310 56-57 1.1965461948230782 2310 58-59 1.1238161685100678 2310 60-61 1.1293616141889586 2310 62-63 0.5428562562632422 2310 64-65 0.7342216092306018 2310 66-67 1.0694775646643109 2310 68-69 1.1964061578640681 2310 70-71 1.2360466062966111 2310 72-73 1.259817537993115 2310 74-75 1.2687596672737342 2310 76-77 1.2449259498149168 2310 78-79 1.1358258698241528 2310 80-81 1.0713192127501046 2310 82-83 1.124914103711557 2310 84-85 1.1759925229150348 2310 86-87 1.159811971023931 2310 88-89 1.195425857346894 2310 90-91 1.2425587570262486 2310 92-93 1.3047545910335607 2310 94-95 1.3668918550386664 2310 96-97 1.324571232453149 2310 98-99 1.321312414214038 2310 100 1.5778658643987313 2311 1 0.40158584982530954 2311 2 0.37118950116717997 2311 3 0.4260997464514702 2311 4 0.2909048989382086 2311 5 0.40331422000884487 2311 6 0.46995377403379734 2311 7 0.49910960984336583 2311 8 0.5264074779730947 2311 9 0.6823838137374594 2311 10-11 0.6743033291006952 2311 12-13 0.7079498674457767 2311 14-15 0.8473705874017838 2311 16-17 0.7017115441778827 2311 18-19 0.8148166416234659 2311 20-21 0.7311132568658678 2311 22-23 0.9415548037546628 2311 24-25 0.9542056647041832 2311 26-27 0.3143185978696508 2311 28-29 0.6729640905587715 2311 30-31 0.9018128226862174 2311 32-33 0.9592183330511759 2311 34-35 1.0222673392402157 2311 36-37 1.1202154784288396 2311 38-39 1.096659386413151 2311 40-41 1.0157598490900952 2311 42-43 1.0316783661129207 2311 44-45 1.0111962509395553 2311 46-47 0.7258980213496926 2311 48-49 0.9436531272325368 2311 50-51 1.0641896837045905 2311 52-53 0.9373826780226366 2311 54-55 1.0148380094269385 2311 56-57 1.1158428633981643 2311 58-59 1.1036596583735019 2311 60-61 1.1333169117223107 2311 62-63 1.1228841279313997 2311 64-65 1.1664753336376492 2311 66-67 1.1580066784003549 2311 68-69 1.1938348257971612 2311 70-71 1.1917389663214237 2311 72-73 1.2255111796116154 2311 74-75 1.2032804590351205 2311 76-77 1.1858730714992411 2311 78-79 0.964033746714513 2311 80-81 0.9440258188265247 2311 82-83 1.0262410083154698 2311 84-85 1.1236252876359991 2311 86-87 1.1122500053706368 2311 88-89 1.1644079485724141 2311 90-91 1.2383482546654783 2311 92-93 1.3395334861800272 2311 94-95 1.2683655320626883 2311 96-97 1.332927043582174 2311 98-99 1.3687308653302246 2311 100 1.5117289949847432 2312 1 0.22650592858592944 2312 2 0.349779000718371 2312 3 0.44003449766160685 2312 4 0.16776695886073156 2312 5 0.3467639541098748 2312 6 0.40299663885230075 2312 7 0.30241605520117076 2312 8 0.41921210963035804 2312 9 0.6050028658388911 2312 10-11 0.6230609705896697 2312 12-13 0.7091929389930058 2312 14-15 0.9071441181612698 2312 16-17 0.8876709506267773 2312 18-19 0.8446656431468682 2312 20-21 0.8431535414816764 2312 22-23 0.9730023594947994 2312 24-25 0.9304103052473423 2312 26-27 0.9791027940505188 2312 28-29 1.0021502555102657 2312 30-31 0.982207030987297 2312 32-33 0.8855082979067319 2312 34-35 0.9624383304989124 2312 36-37 1.0030245426768332 2312 38-39 0.8030300895581561 2312 40-41 0.945750742464682 2312 42-43 1.0432876309335342 2312 44-45 0.8209576977356576 2312 46-47 0.8882726611134473 2312 48-49 1.048977516817068 2312 50-51 0.8961625259205519 2312 52-53 1.046953974771455 2312 54-55 0.9505770100316226 2312 56-57 0.9685675535780476 2312 58-59 1.0574751739283244 2312 60-61 1.0532075178492022 2312 62-63 1.1573166108863049 2312 64-65 1.1798096754191079 2312 66-67 1.1511233728228802 2312 68-69 1.2015368757832974 2312 70-71 1.1314123265546598 2312 72-73 1.1417667954188602 2312 74-75 1.1578600999547959 2312 76-77 1.1744664454491307 2312 78-79 1.085154982730522 2312 80-81 1.0525060525216716 2312 82-83 1.0559389073484162 2312 84-85 1.0788895492072292 2312 86-87 1.1152836567259676 2312 88-89 1.1136063311521518 2312 90-91 1.194858788210464 2312 92-93 1.2323494958242343 2312 94-95 1.2959255461784522 2312 96-97 0.9565346933169536 2312 98-99 0.7288771092908313 2312 100 0.9871917248883264 2313 1 0.06980725758080553 2313 2 0.24226414400434493 2313 3 0.2838207295993129 2313 4 0.20160816131129167 2313 5 0.3208357571110483 2313 6 0.2705305518071768 2313 7 0.27706016548914647 2313 8 0.4049993107145582 2313 9 0.574656813352469 2313 10-11 0.36944084242023223 2313 12-13 0.4305240683401905 2313 14-15 0.48797640568108847 2313 16-17 0.746830025283451 2313 18-19 0.7069399240747885 2313 20-21 0.6617501983658798 2313 22-23 0.6473044655097269 2313 24-25 0.5294721247995184 2313 26-27 0.7509134430288427 2313 28-29 0.5232954173804956 2313 30-31 0.7838629259790935 2313 32-33 0.8784816214369258 2313 34-35 0.9097469013928361 2313 36-37 0.9672639729923205 2313 38-39 0.9395344123105929 2313 40-41 0.93102160083955 2313 42-43 0.9981316235209334 2313 44-45 0.9070688380246139 2313 46-47 0.8521827156982695 2313 48-49 0.9289074781215376 2313 50-51 0.8306857431194956 2313 52-53 0.8452887071372785 2313 54-55 0.8581577904786712 2313 56-57 0.5907165645479466 2313 58-59 0.6343410273306631 2313 60-61 0.5004872634140085 2313 62-63 0.8069916218576836 2313 64-65 0.9278264164252548 2313 66-67 0.9290717346071489 2313 68-69 1.0252662979630216 2313 70-71 0.9890886565906882 2313 72-73 1.0211044594019256 2313 74-75 1.1180778256737511 2313 76-77 1.0237994203346084 2313 78-79 0.9125205378324992 2313 80-81 0.8308288399163644 2313 82-83 0.8817953652262638 2313 84-85 0.9375226302944064 2313 86-87 0.9311551993291722 2313 88-89 0.9090738441530739 2313 90-91 0.8550027960495221 2313 92-93 1.0342879409317831 2313 94-95 1.1347162968125808 2313 96-97 1.210629255111332 2313 98-99 1.22143325942395 2313 100 1.3175772819865266 2314 1 -0.35744915626573004 2314 2 0.03674290434300076 2314 3 0.2986917686250763 2314 4 0.22748914019592092 2314 5 0.3417163875895568 2314 6 0.4210870177325745 2314 7 0.46753026662137387 2314 8 0.4877577646674922 2314 9 0.48219440858014906 2314 10-11 0.583253511938544 2314 12-13 0.5046618532589306 2314 14-15 0.7934434075127399 2314 16-17 0.8310463902919096 2314 18-19 0.8613220491533582 2314 20-21 0.9327297595088879 2314 22-23 0.9711966167304382 2314 24-25 0.8353009816611845 2314 26-27 0.7887292535717023 2314 28-29 0.8250554779715387 2314 30-31 0.8446730075232978 2314 32-33 0.8992176676995953 2314 34-35 0.9567169968404983 2314 36-37 0.8927563986289115 2314 38-39 0.6413616327313036 2314 40-41 0.6812993123221815 2314 42-43 0.8603865692219586 2314 44-45 0.9428989428141463 2314 46-47 0.8621509388415305 2314 48-49 0.9301009328505998 2314 50-51 0.8916288585352348 2314 52-53 0.9027888162982052 2314 54-55 0.8173008827290431 2314 56-57 0.8163624433251542 2314 58-59 0.9013288725601569 2314 60-61 0.9384158692164135 2314 62-63 0.8539620267642434 2314 64-65 0.8848445436298462 2314 66-67 0.6418700536130331 2314 68-69 0.6610298479010979 2314 70-71 0.7736696528379099 2314 72-73 0.912403474910576 2314 74-75 0.9987808039948547 2314 76-77 0.8013329919832621 2314 78-79 0.7748889964031704 2314 80-81 0.8609485832809298 2314 82-83 0.881928585888307 2314 84-85 0.8931750038611632 2314 86-87 0.9545995322984062 2314 88-89 0.9354082639622128 2314 90-91 1.0494227692338747 2314 92-93 0.8941494144157041 2314 94-95 0.9298034589224216 2314 96-97 0.9004237321933459 2314 98-99 1.0081912544186657 2314 100 1.0076340826377255 2315 1 0.43363194149272033 2315 2 0.45952029375298054 2315 3 0.46605500551367385 2315 4 0.2888943260544323 2315 5 0.3751551201041252 2315 6 0.40846998298150083 2315 7 0.34893927024587157 2315 8 0.24906128637714176 2315 9 0.5009371553004982 2315 10-11 0.5939973571334249 2315 12-13 0.3625541191295554 2315 14-15 0.6013119214955722 2315 16-17 0.7537333838930707 2315 18-19 0.7673647636292316 2315 20-21 0.49177590295639106 2315 22-23 0.8456387050545331 2315 24-25 0.8426694009210607 2315 26-27 0.8370698082604022 2315 28-29 0.7885354796719994 2315 30-31 0.8439043394157295 2315 32-33 0.7644514856723319 2315 34-35 0.8465120320482598 2315 36-37 0.8679096145181262 2315 38-39 0.9405602014479655 2315 40-41 0.9468926364986388 2315 42-43 1.0195951605035418 2315 44-45 1.0056658103727756 2315 46-47 1.0248858376445824 2315 48-49 0.8746770375866006 2315 50-51 1.0225534225629005 2315 52-53 1.0347230567687902 2315 54-55 0.8381417059367635 2315 56-57 0.770387382937713 2315 58-59 0.7908790188674502 2315 60-61 0.9300040134292686 2315 62-63 1.0190794732740684 2315 64-65 1.0907485186105816 2315 66-67 1.1195709561969664 2315 68-69 1.154878164715825 2315 70-71 1.0467423210769269 2315 72-73 1.0705630182401222 2315 74-75 0.45748772169881846 2315 76-77 0.5125361324327962 2315 78-79 0.8129806208005732 2315 80-81 0.8497803584864627 2315 82-83 0.8215466570637098 2315 84-85 0.8786557515954136 2315 86-87 0.8939908525399396 2315 88-89 0.9343884187189353 2315 90-91 1.0016141182520677 2315 92-93 1.040829103323194 2315 94-95 1.0172734908491847 2315 96-97 1.1261914074143569 2315 98-99 1.150797811754881 2315 100 1.0599717608596144 2316 1 0.2848163281275333 2316 2 0.05687696113527352 2316 3 0.21663502609192875 2316 4 0.02468035611933317 2316 5 0.24807212093649156 2316 6 0.3464407814867627 2316 7 0.42054234423735437 2316 8 0.4323701514279179 2316 9 0.6107105765106198 2316 10-11 0.6474488087940173 2316 12-13 0.4081339501264054 2316 14-15 0.5513190204404097 2316 16-17 0.7776202078662422 2316 18-19 0.7116462127891054 2316 20-21 0.7350799417996896 2316 22-23 0.7678821941256118 2316 24-25 0.42527241563056606 2316 26-27 0.7404596778763164 2316 28-29 0.9649258661632203 2316 30-31 0.5998325378467584 2316 32-33 0.7042340912228227 2316 34-35 0.8427258936095257 2316 36-37 0.9027623486180261 2316 38-39 0.8451835288259204 2316 40-41 0.8561492921499507 2316 42-43 0.824611210311339 2316 44-45 0.6567262445820674 2316 46-47 0.7997667518511591 2316 48-49 0.9295670347920506 2316 50-51 0.8958579398948885 2316 52-53 0.8641227027162657 2316 54-55 0.7986338019483981 2316 56-57 0.9043966264480829 2316 58-59 0.7901745665911051 2316 60-61 0.8748067022916572 2316 62-63 0.915099236727535 2316 64-65 0.9447461959751351 2316 66-67 0.9372840278991461 2316 68-69 0.9647969740406452 2316 70-71 0.9603293526736678 2316 72-73 0.9249770804374293 2316 74-75 0.951846224560633 2316 76-77 0.8437958318231971 2316 78-79 0.6455612535935344 2316 80-81 0.6469291408362992 2316 82-83 0.5788343182678197 2316 84-85 0.6699719940618216 2316 86-87 0.7018624149743111 2316 88-89 0.71026164548325 2316 90-91 0.6253968751317416 2316 92-93 0.4298591552131832 2316 94-95 0.5077642305642733 2316 96-97 0.706031978517327 2316 98-99 0.8756900185411958 2316 100 0.5469454910348084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 4.0 12 46.0 13 254.0 14 1068.0 15 2919.0 16 7021.0 17 14526.0 18 25736.0 19 38141.0 20 56887.0 21 82167.0 22 99785.0 23 97041.0 24 94905.0 25 102003.0 26 121283.0 27 148001.0 28 183653.0 29 226217.0 30 279578.0 31 349386.0 32 445649.0 33 595767.0 34 870921.0 35 1199496.0 36 1872507.0 37 2475962.0 38 1628303.0 39 260259.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46477145802085 22.044213434219074 13.681581780254037 22.809433327506042 2 15.350607451840709 19.92941173341537 41.31384428114911 23.40613653359481 3 18.81570939124491 26.94483219328525 28.7864837920492 25.452974623420644 4 12.39764289555496 16.453456693952432 39.63600174810539 31.51289866238721 5 12.880745104279933 38.959235320375605 34.25127632043811 13.908743254906359 6 29.788505602422806 38.31542103613682 17.552221974476694 14.34385138696369 7 27.282579506230785 32.036751248891896 23.076708253693305 17.603960991184017 8 26.99324748138104 35.10077357186647 19.136220194255152 18.769758752497342 9 26.59632742327873 15.0765709778447 21.94887808386623 36.37822351501034 10-11 26.62815560254862 26.061749062509072 26.124017210957014 21.186078123985293 12-13 26.343061133770718 23.650267467671863 27.415604813478332 22.591066585079083 14-15 23.707262871561966 24.768087672142276 25.240848911625164 26.283800544670594 16-17 21.73107308448822 27.47600373989336 26.66893095825636 24.123992217362066 18-19 21.138123698717173 27.569719547272264 29.416367018929794 21.87578973508077 20-21 22.97620354246387 26.652170744538715 28.619501903188212 21.7521238098092 22-23 23.410946146081894 26.337344889997294 28.301018795610972 21.95069016830984 24-25 22.705274713632406 26.153322054687617 29.37030270374981 21.771100527930162 26-27 22.560203024308613 26.53335328113658 28.13374780435638 22.772695890198424 28-29 21.539179997659467 27.16559185352516 28.857651239241527 22.437576909573846 30-31 22.758906170844355 26.573462377923544 28.756413795940528 21.91121765529157 32-33 23.18794869317776 26.843398618767516 27.743614678247475 22.225038009807246 34-35 22.995448421854864 26.678226779185255 28.720861149309062 21.60546364965082 36-37 22.514731517625673 26.25400591566523 28.30518195388648 22.92608061282262 38-39 22.829253118067662 26.32949290121289 28.98483407077292 21.85641990994653 40-41 22.26385735260858 25.307826160349673 29.73128207801576 22.697034409025992 42-43 22.40369718958394 25.57601358891648 28.764292594348156 23.255996627151422 44-45 22.533413393482483 26.565661661754675 28.521326167051498 22.379598777711347 46-47 22.56332483761617 26.446069753198575 27.721318486356 23.269286922829256 48-49 23.59685040431201 26.37260859101461 27.720655156970214 22.309885847703164 50-51 22.984875418610695 26.06640519241074 27.75122637821832 23.197493010760244 52-53 22.300730009072357 26.78818618121873 27.39109625139295 23.51998755831596 54-55 22.28229053641431 26.716963566297856 27.37242219338135 23.628323703906485 56-57 23.52889390450143 26.07569883859106 26.91270356218277 23.482703694724737 58-59 22.203413566995707 26.81472010163058 27.827829518573644 23.154036812800076 60-61 22.40650603143493 27.55772600985579 26.98621445197534 23.04955350673394 62-63 22.953628885240036 26.593000112550925 26.503269673128436 23.9501013290806 64-65 21.976325879564726 26.816099749964355 26.961695568469125 24.245878802001794 66-67 22.588717062890964 26.366502223318484 27.053889996754542 23.990890717036013 68-69 22.07870870837305 26.408903870074585 27.32987243814894 24.182514983403422 70-71 21.968521523899003 25.929751149765007 27.59597903350906 24.50574829282693 72-73 22.72271711144017 26.13509410542822 27.704597799073316 23.437590984058296 74-75 22.963550998089524 26.596172318014517 27.14854414227562 23.29173254162034 76-77 22.635097354203708 26.376789692908414 26.656634639917907 24.331478312969967 78-79 22.460519452029917 26.45505950689361 26.817400675612348 24.267020365464127 80-81 21.88996468286636 27.038989191727424 27.437659525206616 23.633386600199593 82-83 23.127854331821666 25.733645733255493 28.67230846085093 22.46619147407191 84-85 23.34697771050772 26.296315991552145 27.038046882945117 23.318659414995018 86-87 21.68681576323951 27.189695130703008 27.592561741980504 23.530927364076984 88-89 22.816245729930813 25.744190512345313 27.426685048515402 24.012878709208472 90-91 22.46858554257509 25.767689549042416 27.61955380252077 24.144171105861727 92-93 22.93199324825163 26.108616975840942 27.317756075595707 23.641633700311722 94-95 21.59731331355704 26.061361246247834 28.101859361978914 24.239466078216214 96-97 21.977021583132466 26.020637796802703 27.602121186933754 24.400219433131078 98-99 21.26498101377006 26.636415625382142 27.448375021515304 24.650228339332493 100 22.054990015021367 25.90105585894495 26.605263349266377 25.43869077676731 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1458.0 1 999.0 2 817.0 3 1051.0 4 826.5 5 767.0 6 1244.5 7 2497.0 8 3923.5 9 4471.0 10 4047.5 11 3629.5 12 4138.0 13 5217.5 14 6531.5 15 8182.0 16 9872.5 17 11558.0 18 13286.0 19 14706.0 20 15782.0 21 16735.5 22 18130.5 23 19938.5 24 21422.5 25 23380.0 26 26987.0 27 30815.5 28 37438.5 29 54654.5 30 81314.5 31 107622.0 32 146680.5 33 193124.0 34 227324.0 35 262386.0 36 294282.0 37 347705.5 38 370315.0 39 342083.5 40 308168.0 41 273393.0 42 263688.0 43 278232.5 44 312512.5 45 386230.0 46 454990.5 47 502130.5 48 540420.5 49 534555.5 50 509329.5 51 527417.5 52 538007.0 53 507422.0 54 482355.5 55 417360.0 56 359425.5 57 296767.5 58 232066.5 59 226328.0 60 186477.5 61 121050.5 62 86167.0 63 73601.0 64 53492.0 65 23344.0 66 11651.0 67 7517.5 68 5731.5 69 4839.5 70 4020.5 71 3133.5 72 2560.0 73 2471.5 74 1987.0 75 1450.5 76 989.0 77 576.0 78 352.5 79 231.5 80 145.5 81 99.5 82 74.0 83 42.5 84 23.5 85 13.0 86 7.5 87 7.5 88 5.5 89 4.5 90 4.5 91 4.0 92 2.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043955868591634646 2 0.0 3 0.0 4 0.0291413755669026 5 0.0 6 0.0 7 8.511019331982504E-4 8 0.0 9 0.010647639810115612 10-11 4.654463697177932E-4 12-13 8.865645137481775E-6 14-15 1.5071596733719016E-4 16-17 2.571037089869715E-4 18-19 4.432822568740888E-6 20-21 8.865645137481775E-6 22-23 0.0 24-25 5.984310467800198E-4 26-27 5.097745954052021E-4 28-29 1.3298467706222665E-4 30-31 0.00259763402528216 32-33 0.0160778474568232 34-35 0.027275157265462684 36-37 0.032545783299695596 38-39 0.023396437517814405 40-41 0.0014007719317221206 42-43 7.535798366859508E-5 44-45 0.011596263839826162 46-47 0.018156841241562676 48-49 0.003515228297011524 50-51 0.011219473921483187 52-53 0.011339160130839191 54-55 0.010040343118198111 56-57 4.299837891678661E-4 58-59 0.008861212314913035 60-61 0.004836209422496309 62-63 0.001387473464015898 64-65 0.036371309176518984 66-67 0.060450401369919485 68-69 0.0593023003246156 70-71 0.05219205292435521 72-73 0.04608805624719901 74-75 0.03571525143634533 76-77 0.031987247656034244 78-79 0.015302103507293545 80-81 0.0011126384647539627 82-83 0.039163987394825746 84-85 0.05199257590876187 86-87 0.05791925968316844 88-89 0.06975932876427535 90-91 0.04951906091540446 92-93 0.03980231384472443 94-95 0.013418153915578667 96-97 0.0038343915219608677 98-99 0.023387571872676923 100 0.031535099754022675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1279495E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.56422135046059 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88181790938344 46.10040812898166 2 14.93542603930906 18.389757492949116 3 5.108938654155465 9.435834905110537 4 2.1636424037167403 5.3281183946264 5 1.052242771422551 3.239025344714002 6 0.5803095630509657 2.1435783834875233 7 0.3534529826226612 1.5232040361413413 8 0.21746334603477474 1.071036925671734 9 0.13908383193663745 0.7706329034055666 >10 0.5226069871124712 5.224475297842157 >50 0.024449376693239593 1.0429409246586696 >100 0.01773879090987275 2.1253739791827804 >500 0.0016240885344342513 0.7000723055895764 >1k 9.93163012305031E-4 1.1709647146317854 >5k 1.3369497619199005E-4 0.5489726514365939 >10k+ 7.639712925256574E-5 1.1856036115704935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36366 0.32240805106966225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29906 0.26513598348152995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18932 0.167844393742805 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 15627 0.1385434365634277 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.865645137481775E-6 0.0 6 0.0 0.0 0.0 8.865645137481775E-6 0.0 7 0.0 0.0 0.0 8.865645137481775E-6 0.0 8 0.0 0.0 0.0 1.773129027496355E-5 0.0 9 0.0 0.0 0.0 2.6596935412445328E-5 0.0 10-11 0.0 0.0 0.0 2.6596935412445328E-5 0.0 12-13 0.0 0.0 0.0 2.6596935412445328E-5 0.0 14-15 0.0 0.0 0.0 3.989540311866799E-5 0.0 16-17 0.0 0.0 0.0 5.762669339363154E-5 0.0 18-19 0.0 0.0 0.0 6.205951596237243E-5 0.0 20-21 0.0 0.0 0.0 9.308927394355865E-5 0.0 22-23 0.0 0.0 0.0 2.0834266073082173E-4 0.0 24-25 0.0 0.0 0.0 5.452371759551292E-4 0.0 26-27 0.0 0.0 0.0 0.0012633544320911532 0.0 28-29 0.0 0.0 0.0 0.003829958699392127 0.0 30-31 0.0 0.0 0.0 0.014552956493176335 0.0 32-33 0.0 0.0 0.0 0.04106123545424684 0.0 34-35 0.0 0.0 0.0 0.07882445091735046 0.0 36-37 0.0 0.0 0.0 0.14450115009581546 4.432822568740888E-6 38-39 0.0 0.0 0.0 0.24440367232752885 8.865645137481775E-6 40-41 0.0 0.0 0.0 0.35202817147398885 8.865645137481775E-6 42-43 0.0 0.0 0.0 0.4561817705491248 8.865645137481775E-6 44-45 0.0 0.0 0.0 0.5758280845020101 8.865645137481775E-6 46-47 4.432822568740888E-6 0.0 0.0 0.7196155501642583 8.865645137481775E-6 48-49 8.865645137481775E-6 0.0 0.0 0.8809215306181704 8.865645137481775E-6 50-51 8.865645137481775E-6 0.0 0.0 1.0495328026653676 8.865645137481775E-6 52-53 8.865645137481775E-6 0.0 0.0 1.2086888641734403 8.865645137481775E-6 54-55 8.865645137481775E-6 0.0 0.0 1.3776370307358619 8.865645137481775E-6 56-57 8.865645137481775E-6 0.0 0.0 1.5763693321376535 8.865645137481775E-6 58-59 8.865645137481775E-6 0.0 0.0 1.809708679333605 8.865645137481775E-6 60-61 8.865645137481775E-6 0.0 0.0 2.0454594820069514 8.865645137481775E-6 62-63 8.865645137481775E-6 0.0 0.0 2.2878949811139595 8.865645137481775E-6 64-65 8.865645137481775E-6 0.0 0.0 2.5308180907035287 8.865645137481775E-6 66-67 8.865645137481775E-6 0.0 0.0 2.80130005820296 8.865645137481775E-6 68-69 1.773129027496355E-5 0.0 0.0 3.0997309719983033 8.865645137481775E-6 70-71 1.773129027496355E-5 0.0 0.0 3.4233713477420755 8.865645137481775E-6 72-73 1.773129027496355E-5 0.0 0.0 3.7525394532290672 8.865645137481775E-6 74-75 1.773129027496355E-5 0.0 0.0 4.064574699487876 8.865645137481775E-6 76-77 1.773129027496355E-5 0.0 0.0 4.419098550068066 8.865645137481775E-6 78-79 1.773129027496355E-5 0.0 0.0 4.794359144624826 8.865645137481775E-6 80-81 1.773129027496355E-5 0.0 0.0 5.197218492494566 8.865645137481775E-6 82-83 1.773129027496355E-5 0.0 0.0 5.607325505264198 8.865645137481775E-6 84-85 1.773129027496355E-5 0.0 0.0 5.996456401638548 8.865645137481775E-6 86-87 1.773129027496355E-5 0.0 0.0 6.40550840263682 8.865645137481775E-6 88 1.773129027496355E-5 0.0 0.0 6.736312219651678 8.865645137481775E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 14335 0.0 42.107365 94 GGTATCA 28225 0.0 36.612453 1 GTACTAG 13730 0.0 36.023033 1 GTATCAA 45520 0.0 35.095882 1 TATTCTC 5435 0.0 33.629417 5 TAGGCAT 16535 0.0 33.303715 5 CTGTGCG 20085 0.0 32.821102 9 ACCTGGG 4565 0.0 32.327473 3 TAGTACT 20710 0.0 31.861773 4 ACTGTGC 21145 0.0 31.753521 8 TACCTGG 4935 0.0 30.376673 2 GTACTGT 21925 0.0 30.259682 6 CAACGCA 50245 0.0 30.223711 5 TACTGTG 23250 0.0 29.99026 7 ATCAACG 51080 0.0 29.691422 3 CTAGGCA 19065 0.0 29.63129 4 TACCCCG 685 0.0 29.494917 5 CTAGTAC 24195 0.0 28.729328 3 TATCAAC 57240 0.0 27.872557 2 CTAACTG 5175 0.0 27.245438 4 >>END_MODULE