FastQCFastQC Report
Mon 6 Jun 2016
ERR1211196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571885
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278710.36808535787323765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228560.3018535014728829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134800.1780270038438249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153900.046.7310751
GTATCAA272100.040.615391
TACCTGG47300.034.9276962
GTACTAG66000.034.302221
CAACGCA315600.033.6778835
ATCAACG318050.033.4036753
TATCAAC344650.031.8337632
CTGTGCG92200.030.7426859
AACGCAG356700.030.7069666
GGGCACC81800.030.4501294
GCATCGT68700.030.379778
TAGTACT95250.030.2042224
ACCTGGG54000.030.0335453
GTGGTAT50750.029.770771
TAGGCAT81350.029.644295
TCAACGC366550.029.2788454
GTACATG400150.028.8533231
TACATGG403200.028.2150022
ACTGTGC107600.028.046658
ACATGGG399100.027.66833
Produced by FastQC (version 0.11.5)