FastQCFastQC Report
Mon 6 Jun 2016
ERR1211196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571885
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250960.33143662377334043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214870.28377345931693365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130170.17191227811832852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156250.045.6750951
GTATCAA263600.038.3817331
TACCTGG46650.036.5661282
GTACTAG67600.035.3996351
CAACGCA309000.031.3709375
GGGCACC78800.031.19998494
ATCAACG313700.030.9086783
TAGGCAT83950.030.6723165
CTGTGCG91850.030.080299
ACCTGGG55000.029.8234883
TAGTACT94100.029.818134
TATCAAC338850.029.7747782
GTGGTAT50150.028.6865431
GTACATG392750.028.3341241
AACGCAG350750.028.2932556
TACATGG395000.028.0048582
CTAGGCA93900.027.9295734
ACTGTGC107100.027.5520558
ACATGGG387250.027.2835333
TCAACGC362150.027.1499444
Produced by FastQC (version 0.11.5)