FastQCFastQC Report
Mon 6 Jun 2016
ERR1211195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056094
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166830.3299582642253091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133990.26500694013995785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.16487035248949092No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84000.048.852551
TACCTGG45250.042.1189882
GTATCAA147150.039.72021
GTACCTG55150.035.0708581
CAACGCA168050.033.4833155
ACCTGGG55200.033.46743
ATCAACG170200.033.0600173
GTACTAG27950.032.158931
TATCAAC182300.031.6221142
AACGCAG184400.031.228586
TCAACGC186600.030.3811474
GTACATG173250.028.3037871
GCATCGT27350.028.1857248
ACGCAGA201250.027.9365237
CTGTGCG34150.027.8040229
TACATGG174950.027.517132
CGCAGAG205000.027.400398
TAGTACT38400.026.8090464
ACATGGG174600.026.3307423
GTGGTAT33350.025.9640271
Produced by FastQC (version 0.11.5)