FastQCFastQC Report
Mon 6 Jun 2016
ERR1211195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056094
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148590.2938829855615817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128160.25347630008461075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.1655230302284728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.046.3851551
TACCTGG42400.041.674062
GTATCAA141800.038.823651
GTACCTG51200.035.1677441
ACCTGGG51500.033.6758733
CAACGCA169850.031.3708955
ATCAACG169600.031.3704173
TATCAAC180700.030.2198162
AACGCAG186400.029.240956
TCAACGC185750.028.8706464
CTGTGCG38100.027.255039
GTACATG168950.026.6855471
GTGGTAT36150.026.660071
CCTGGGG49900.026.1844794
ACGCAGA204650.026.1282257
TACATGG170650.025.8860282
CTAGTAC42850.025.6663843
CGCAGAG209000.025.606898
TAGTACT42900.025.5269134
GTACTAG27100.025.1544271
Produced by FastQC (version 0.11.5)