FastQCFastQC Report
Mon 6 Jun 2016
ERR1211194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187850.31080826860849725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151210.2501853515905822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92210.15256657145802252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99700.046.5025371
GTATCAA171900.040.908191
TACCTGG55650.039.751862
ATCAACG202050.033.851833
CAACGCA203850.033.806585
ACCTGGG65300.032.8988723
TATCAAC212200.032.761212
GTACCTG69850.032.7495571
AACGCAG225350.031.2278586
TCAACGC221900.031.0990224
GTACATG208600.029.6268841
GTGGTAT40950.028.620751
TACATGG212750.028.5836522
ACGCAGA245450.028.2494857
GTACTAG29700.028.051261
CGCAGAG250500.027.7149948
ACATGGG217700.027.0780323
CATGGGG169150.025.7067814
ATGGGGG129750.025.4335925
GCAGAGT268750.025.133149
Produced by FastQC (version 0.11.5)